LOCUS       DAJTNV010000001        75860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNV010000001 DAJTNV010000000
VERSION     DAJTNV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..201)
                     /locus_tag="OO199_000001"
     CDS             complement(<1..201)
                     /locus_tag="OO199_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534210.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLH"
     gene            complement(203..423)
                     /locus_tag="OO199_000002"
                     /pseudo
     CDS             complement(203..423)
                     /locus_tag="OO199_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(416..637)
                     /locus_tag="OO199_000003"
     CDS             complement(416..637)
                     /locus_tag="OO199_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534211.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(627..827)
                     /locus_tag="OO199_000004"
     CDS             complement(627..827)
                     /locus_tag="OO199_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5534212.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            957..1136
                     /locus_tag="OO199_000005"
     CDS             957..1136
                     /locus_tag="OO199_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534213.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1261..1590
                     /locus_tag="OO199_000006"
     CDS             1261..1590
                     /locus_tag="OO199_000006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534214.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1640..1828)
                     /locus_tag="OO199_000007"
     CDS             complement(1640..1828)
                     /locus_tag="OO199_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5534215.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2203..3431)
                     /locus_tag="OO199_000008"
                     /pseudo
     CDS             complement(2203..3431)
                     /locus_tag="OO199_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3680..4801
                     /gene="roxA"
                     /locus_tag="OO199_000009"
                     /gene_synonym="ycfD"
     CDS             3680..4801
                     /gene="roxA"
                     /locus_tag="OO199_000009"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5534216.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4850..6076)
                     /gene="pepT"
                     /locus_tag="OO199_000010"
     CDS             complement(4850..6076)
                     /gene="pepT"
                     /locus_tag="OO199_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5534217.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6326..7462
                     /gene="potA"
                     /locus_tag="OO199_000011"
     CDS             6326..7462
                     /gene="potA"
                     /locus_tag="OO199_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5534218.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7446..8303
                     /gene="potB"
                     /locus_tag="OO199_000012"
     CDS             7446..8303
                     /gene="potB"
                     /locus_tag="OO199_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5534219.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8300..9094
                     /gene="potC"
                     /locus_tag="OO199_000013"
     CDS             8300..9094
                     /gene="potC"
                     /locus_tag="OO199_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5534220.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9091..10137
                     /gene="potD"
                     /locus_tag="OO199_000014"
     CDS             9091..10137
                     /gene="potD"
                     /locus_tag="OO199_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5534221.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10293..11114)
                     /gene="cobB"
                     /locus_tag="OO199_000015"
     CDS             complement(10293..11114)
                     /gene="cobB"
                     /locus_tag="OO199_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5534222.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11130..12041)
                     /gene="nagK"
                     /locus_tag="OO199_000016"
     CDS             complement(11130..12041)
                     /gene="nagK"
                     /locus_tag="OO199_000016"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5534223.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12070..13314)
                     /gene="lolE"
                     /locus_tag="OO199_000017"
     CDS             complement(12070..13314)
                     /gene="lolE"
                     /locus_tag="OO199_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5534224.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13314..14015)
                     /gene="lolD"
                     /locus_tag="OO199_000018"
     CDS             complement(13314..14015)
                     /gene="lolD"
                     /locus_tag="OO199_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5534225.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14008..15207)
                     /gene="lolC"
                     /locus_tag="OO199_000019"
     CDS             complement(14008..15207)
                     /gene="lolC"
                     /locus_tag="OO199_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5534226.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15469..16542
                     /locus_tag="OO199_000020"
     CDS             15469..16542
                     /locus_tag="OO199_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5534227.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16670..20116
                     /gene="mfd"
                     /locus_tag="OO199_000021"
     CDS             16670..20116
                     /gene="mfd"
                     /locus_tag="OO199_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5534228.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20259..21221
                     /gene="ldtC"
                     /locus_tag="OO199_000022"
     CDS             20259..21221
                     /gene="ldtC"
                     /locus_tag="OO199_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5534229.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21304..21516)
                     /locus_tag="OO199_000023"
     CDS             complement(21304..21516)
                     /locus_tag="OO199_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5534230.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21801..22433
                     /gene="comR"
                     /locus_tag="OO199_000024"
     CDS             21801..22433
                     /gene="comR"
                     /locus_tag="OO199_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5534231.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22495..23034)
                     /locus_tag="OO199_000025"
     CDS             complement(22495..23034)
                     /locus_tag="OO199_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5534232.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23261..24565)
                     /gene="ndh"
                     /locus_tag="OO199_000026"
     CDS             complement(23261..24565)
                     /gene="ndh"
                     /locus_tag="OO199_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5534233.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24973..25515)
                     /locus_tag="OO199_000027"
     CDS             complement(24973..25515)
                     /locus_tag="OO199_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5534234.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25538..26563)
                     /gene="nagZ"
                     /locus_tag="OO199_000028"
     CDS             complement(25538..26563)
                     /gene="nagZ"
                     /locus_tag="OO199_000028"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5534235.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26574..27398)
                     /gene="thiK"
                     /locus_tag="OO199_000029"
     CDS             complement(26574..27398)
                     /gene="thiK"
                     /locus_tag="OO199_000029"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5534236.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27379..28020)
                     /gene="lpoB"
                     /locus_tag="OO199_000030"
     CDS             complement(27379..28020)
                     /gene="lpoB"
                     /locus_tag="OO199_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5534237.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28035..28412)
                     /locus_tag="OO199_000031"
     CDS             complement(28035..28412)
                     /locus_tag="OO199_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5534238.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28415..28774)
                     /gene="hinT"
                     /locus_tag="OO199_000032"
     CDS             complement(28415..28774)
                     /gene="hinT"
                     /locus_tag="OO199_000032"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5534239.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29113..31298
                     /gene="fhuE"
                     /locus_tag="OO199_000033"
                     /pseudo
     CDS             29113..31298
                     /gene="fhuE"
                     /locus_tag="OO199_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31358..32791)
                     /gene="ptsG"
                     /locus_tag="OO199_000034"
     CDS             complement(31358..32791)
                     /gene="ptsG"
                     /locus_tag="OO199_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5534240.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33086..33883)
                     /locus_tag="OO199_000035"
     CDS             complement(33086..33883)
                     /locus_tag="OO199_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5534241.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33894..34898)
                     /gene="holB"
                     /locus_tag="OO199_000036"
     CDS             complement(33894..34898)
                     /gene="holB"
                     /locus_tag="OO199_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5534242.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34895..35536)
                     /locus_tag="OO199_000037"
     CDS             complement(34895..35536)
                     /locus_tag="OO199_000037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5534243.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35526..36548)
                     /gene="yceG"
                     /locus_tag="OO199_000038"
     CDS             complement(35526..36548)
                     /gene="yceG"
                     /locus_tag="OO199_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5534244.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36551..37360)
                     /gene="pabC"
                     /locus_tag="OO199_000039"
     CDS             complement(36551..37360)
                     /gene="pabC"
                     /locus_tag="OO199_000039"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5534245.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37480..38721)
                     /gene="fabF"
                     /locus_tag="OO199_000040"
     CDS             complement(37480..38721)
                     /gene="fabF"
                     /locus_tag="OO199_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5534246.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38808..39044)
                     /gene="acpP"
                     /locus_tag="OO199_000041"
     CDS             complement(38808..39044)
                     /gene="acpP"
                     /locus_tag="OO199_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5534247.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39255..39989)
                     /gene="fabG"
                     /locus_tag="OO199_000042"
     CDS             complement(39255..39989)
                     /gene="fabG"
                     /locus_tag="OO199_000042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5534248.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40002..40931)
                     /gene="fabD"
                     /locus_tag="OO199_000043"
     CDS             complement(40002..40931)
                     /gene="fabD"
                     /locus_tag="OO199_000043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5534249.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40947..41900)
                     /gene="fabH"
                     /locus_tag="OO199_000044"
     CDS             complement(40947..41900)
                     /gene="fabH"
                     /locus_tag="OO199_000044"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5534250.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41968..43038)
                     /gene="plsX"
                     /locus_tag="OO199_000045"
     CDS             complement(41968..43038)
                     /gene="plsX"
                     /locus_tag="OO199_000045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5534251.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43119..43292)
                     /gene="rpmF"
                     /locus_tag="OO199_000046"
     CDS             complement(43119..43292)
                     /gene="rpmF"
                     /locus_tag="OO199_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5534252.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43344..43865)
                     /gene="yceD"
                     /locus_tag="OO199_000047"
     CDS             complement(43344..43865)
                     /gene="yceD"
                     /locus_tag="OO199_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5534253.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44064..44648
                     /gene="yceF"
                     /locus_tag="OO199_000048"
     CDS             44064..44648
                     /gene="yceF"
                     /locus_tag="OO199_000048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5534254.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44760..45719)
                     /gene="rluC"
                     /locus_tag="OO199_000049"
     CDS             complement(44760..45719)
                     /gene="rluC"
                     /locus_tag="OO199_000049"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5534255.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46292..49477
                     /gene="rne"
                     /locus_tag="OO199_000050"
     CDS             46292..49477
                     /gene="rne"
                     /locus_tag="OO199_000050"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5534256.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49673..50626)
                     /gene="flgL"
                     /locus_tag="OO199_000051"
     CDS             complement(49673..50626)
                     /gene="flgL"
                     /locus_tag="OO199_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5534257.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50638..52280)
                     /gene="flgK"
                     /locus_tag="OO199_000052"
                     /pseudo
     CDS             complement(50638..52280)
                     /gene="flgK"
                     /locus_tag="OO199_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52346..53287)
                     /gene="flgJ"
                     /locus_tag="OO199_000053"
     CDS             complement(52346..53287)
                     /gene="flgJ"
                     /locus_tag="OO199_000053"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5534258.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53287..54384)
                     /gene="flgI"
                     /locus_tag="OO199_000054"
     CDS             complement(53287..54384)
                     /gene="flgI"
                     /locus_tag="OO199_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5534259.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54396..55094)
                     /gene="flgH"
                     /locus_tag="OO199_000055"
     CDS             complement(54396..55094)
                     /gene="flgH"
                     /locus_tag="OO199_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5534260.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55147..55929)
                     /gene="flgG"
                     /locus_tag="OO199_000056"
     CDS             complement(55147..55929)
                     /gene="flgG"
                     /locus_tag="OO199_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5534261.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56067..56818)
                     /gene="flgF"
                     /locus_tag="OO199_000057"
                     /pseudo
     CDS             complement(56067..56818)
                     /gene="flgF"
                     /locus_tag="OO199_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56838..58046)
                     /gene="flgE"
                     /locus_tag="OO199_000058"
     CDS             complement(56838..58046)
                     /gene="flgE"
                     /locus_tag="OO199_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5534262.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58071..58766)
                     /gene="flgD"
                     /locus_tag="OO199_000059"
     CDS             complement(58071..58766)
                     /gene="flgD"
                     /locus_tag="OO199_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5534263.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58778..59181)
                     /gene="flgC"
                     /locus_tag="OO199_000060"
                     /pseudo
     CDS             complement(58778..59181)
                     /gene="flgC"
                     /locus_tag="OO199_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59185..59601)
                     /gene="flgB"
                     /locus_tag="OO199_000061"
     CDS             complement(59185..59601)
                     /gene="flgB"
                     /locus_tag="OO199_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5534264.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59756..60415
                     /gene="flgA"
                     /locus_tag="OO199_000062"
     CDS             59756..60415
                     /gene="flgA"
                     /locus_tag="OO199_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5534265.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60491..60784
                     /gene="flgM"
                     /locus_tag="OO199_000063"
     CDS             60491..60784
                     /gene="flgM"
                     /locus_tag="OO199_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5534266.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60789..61205
                     /gene="flgN"
                     /locus_tag="OO199_000064"
     CDS             60789..61205
                     /gene="flgN"
                     /locus_tag="OO199_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5534267.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61245..62780)
                     /gene="murJ"
                     /locus_tag="OO199_000065"
     CDS             complement(61245..62780)
                     /gene="murJ"
                     /locus_tag="OO199_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5534268.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62947..63813)
                     /locus_tag="OO199_000066"
     CDS             complement(62947..63813)
                     /locus_tag="OO199_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5534269.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63815..64462)
                     /locus_tag="OO199_000067"
                     /pseudo
     CDS             complement(63815..64462)
                     /locus_tag="OO199_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64473..65057)
                     /gene="rimJ"
                     /locus_tag="OO199_000068"
     CDS             complement(64473..65057)
                     /gene="rimJ"
                     /locus_tag="OO199_000068"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5534270.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65293..66501
                     /gene="mdtH"
                     /locus_tag="OO199_000069"
     CDS             65293..66501
                     /gene="mdtH"
                     /locus_tag="OO199_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5534271.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66565..67212
                     /gene="grxB"
                     /locus_tag="OO199_000070"
     CDS             66565..67212
                     /gene="grxB"
                     /locus_tag="OO199_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5534272.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67346..67906
                     /locus_tag="OO199_000071"
     CDS             67346..67906
                     /locus_tag="OO199_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5534273.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68012..69058
                     /gene="pyrC"
                     /locus_tag="OO199_000072"
     CDS             68012..69058
                     /gene="pyrC"
                     /locus_tag="OO199_000072"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5534274.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69132..69377
                     /gene="dinI"
                     /locus_tag="OO199_000073"
     CDS             69132..69377
                     /gene="dinI"
                     /locus_tag="OO199_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5534275.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69667..69921
                     /gene="bssS"
                     /locus_tag="OO199_000074"
     CDS             69667..69921
                     /gene="bssS"
                     /locus_tag="OO199_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5534276.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70036..71154
                     /gene="solA"
                     /locus_tag="OO199_000075"
     CDS             70036..71154
                     /gene="solA"
                     /locus_tag="OO199_000075"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5534277.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71202..71315
                     /locus_tag="OO199_000076"
     CDS             71202..71315
                     /locus_tag="OO199_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5534278.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71576..72142
                     /locus_tag="OO199_000077"
     CDS             71576..72142
                     /locus_tag="OO199_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5534279.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72146..72721
                     /locus_tag="OO199_000078"
     CDS             72146..72721
                     /locus_tag="OO199_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5534280.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72763..73815)
                     /locus_tag="OO199_000079"
     CDS             complement(72763..73815)
                     /locus_tag="OO199_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5534281.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74040..74960
                     /gene="lpxL"
                     /locus_tag="OO199_000080"
     CDS             74040..74960
                     /gene="lpxL"
                     /locus_tag="OO199_000080"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5534282.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75132..75329
                     /locus_tag="OO199_000081"
     CDS             75132..75329
                     /locus_tag="OO199_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5534283.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75353..>75860
                     /locus_tag="OO199_000082"
     CDS             75353..>75860
                     /locus_tag="OO199_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5534284.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKV"
BASE COUNT        18493 a        20066 c        18763 g        18538 t
ORIGIN      
        1 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
       61 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
      121 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
      181 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
      241 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
      301 tctcgtggaa agtaagagaa aagccgctct aataagttct ggcattgacg aaagcgcatc
      361 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
      421 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
      481 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
      541 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
      601 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
      661 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
      721 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
      781 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
      841 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
      901 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
      961 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
     1021 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
     1081 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
     1141 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
     1201 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
     1261 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
     1321 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
     1381 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
     1441 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
     1501 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
     1561 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
     1621 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
     1681 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
     1741 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
     1801 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
     1861 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
     1921 gttgaggccg catggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
     1981 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
     2041 cgctagtgta taaaaaatca gcaacacatt aaaaatcagc tatttataaa tctgtacact
     2101 ttttcacctc ttgttcgtag ttgttccgct ttgttgctca ttgttgcacg ttgtatctgt
     2161 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
     2221 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
     2281 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
     2341 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
     2401 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
     2461 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
     2521 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
     2581 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
     2641 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
     2701 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
     2761 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gttttttccc
     2821 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
     2881 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
     2941 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
     3001 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
     3061 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
     3121 taacgcttcc tgtacggtgt aattaagctc atgacgtgga ttttcccctc tgccagccat
     3181 gcgcgacact gggcggcttt ttccctggct gatttcaggt tcagatcagg ataatttccc
     3241 agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt gaaataccag
     3301 gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt aagaaaggct
     3361 gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag tttgttaagt
     3421 gctctgctca taattttgat tccggtcatc acaattaact acaccattaa ctacaccgat
     3481 cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac aaatctgttt
     3541 ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa cactaattga
     3601 taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     3661 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     3721 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     3781 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca gtcgactggt
     3841 cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct acgatcatct
     3901 cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg agccgaccgc
     3961 cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc tgatgatttc
     4021 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     4081 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     4141 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     4201 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     4261 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa ttagcggatt
     4321 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     4381 acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc gtgagatgat
     4441 gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt ttatatccca
     4501 gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta
     4561 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat
     4621 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     4681 tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc
     4741 gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct tcgaagggta
     4801 agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct tacttccgtt
     4861 gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata ccttccagaa
     4921 tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc gggcaaggta
     4981 atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc agttccggtt
     5041 caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga tgctcaacca
     5101 ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag caatcaggat
     5161 gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc gcttcaaact
     5221 gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg gtgcctttca
     5281 tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt tcatccgccg
     5341 gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact cctttcgccg
     5401 tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg ttgaagtttt
     5461 caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg gcatcgaagg
     5521 catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta aaggcgacgc
     5581 gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc atgatttctg
     5641 caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta atcaacgtct
     5701 gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca tcgccgatac
     5761 ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca tttttgccgc
     5821 tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata tcgccaggga
     5881 cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca ttgataagcc
     5941 ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct tccgtgctgg
     6001 gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc aaaaatcgct
     6061 caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta agaagcaaga
     6121 aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt tatcagcatt
     6181 accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga ttcaagcggt
     6241 ttgccgtaga attaccgccc tcattaagag tcaccaaggt ggttaaccac aaaccccgca
     6301 tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata aacaaccgag
     6361 ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg gtaaagaggt
     6421 cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc
     6481 tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg ttgattccgg
     6541 acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc gctatgtgaa
     6601 cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa atgtggcctt
     6661 tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga tggaagccct
     6721 gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct ctggtggtca
     6781 acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga
     6841 tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg agctgaaagc
     6901 gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg aagaagcact
     6961 caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag acggcacgcc
     7021 gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg gcgaaattaa
     7081 tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg ccaacgttga
     7141 aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa aactgcatgt
     7201 tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc acgctgaagg
     7261 gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt cggttgttga
     7321 actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg atcctgactt
     7381 tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg aggtcgtact
     7441 ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt gtcggttggc
     7501 ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt ttgacccgcg
     7561 acgacgccag tttcgtcaaa atggtcttta cgctggataa ctacacgcgt ctgctcgatc
     7621 cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc accctcgcct
     7681 gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc
     7741 cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt cgtatctacg
     7801 ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc tggctgggcg
     7861 ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc ggtctggttt
     7921 acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa ctggataagc
     7981 cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt atccgcatca
     8041 ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg ctgccagcga
     8101 tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg
     8161 tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc accagcatta
     8221 cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct cgtttgctga
     8281 ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta tgaccgctat
     8341 ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta acagctcgcg
     8401 ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga tgaacaacga
     8461 cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg cgacgtttgc
     8521 tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc
     8581 gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga tggcaatttc
     8641 gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc tgctgttctc
     8701 gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt
     8761 tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta ccattctgcg
     8821 aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt taagctttac
     8881 cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt atgaaattct
     8941 gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc
     9001 aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta ttgctcgtga
     9061 taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg
     9121 gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa cacgctgtat
     9181 ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac caaagaaacc
     9241 ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa
     9301 acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt cgataaaatg
     9361 cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt cagcaatctc
     9421 gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat tccgtatatc
     9481 tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc tgtcaccagc
     9541 tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga cgatgcccgt
     9601 gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac cgatccgaaa
     9661 gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac
     9721 tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat gatctggaac
     9781 ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa
     9841 ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa caaagaaggc
     9901 gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact
     9961 atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga agtggcaaac
    10021 gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt
    10081 ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg acgttaattc
    10141 agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg gctaatgagt
    10201 caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt gtaggcctga
    10261 taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa cttttcaaca
    10321 aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc attaccaacc
    10381 tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc ttcgtgtaca
    10441 aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac atccgccatc
    10501 gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc
    10561 gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg
    10621 gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata
    10681 tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc
    10741 aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc
    10801 tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca
    10861 ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg
    10921 ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc ccctgtcagt
    10981 acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc
    11041 tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa ccgatgaccc
    11101 cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca
    11161 cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt
    11221 aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa attcgataag
    11281 ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc cagagaaacc
    11341 gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa
    11401 agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca
    11461 aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt
    11521 aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac
    11581 tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag cccgccgcca
    11641 acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc atcatcccag
    11701 gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt
    11761 gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc ggcatacagc
    11821 ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca
    11881 aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg
    11941 tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca
    12001 agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat
    12061 gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc
    12121 cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc
    12181 cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg agaggaactg
    12241 atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg aaacaactac
    12301 gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa
    12361 gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact
    12421 cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac
    12481 cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc
    12541 gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg
    12601 taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga
    12661 acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag
    12721 ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag
    12781 tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct gcttcacttt
    12841 cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg
    12901 ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt
    12961 aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac cggtgaaatt
    13021 gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca gtgcttcctg
    13081 ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca ccgccagaat
    13141 gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc
    13201 cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg
    13261 tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc
    13321 gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag ttggcggctc
    13381 atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc ctgcaagcga
    13441 ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta
    13501 ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat cgctacacgc
    13561 tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc
    13621 gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt gccaatcagc
    13681 agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg aaactgataa
    13741 ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag tttgctcatc
    13801 ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc taacaggtgc
    13861 agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc accttcgccc
    13921 acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg atagcgtttg
    13981 cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgtgt
    14041 ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag
    14101 cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc catcaagcag
    14161 gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc
    14221 tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga ccatcatgat
    14281 ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccatcac
    14341 catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca ggctcagcag
    14401 taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg
    14461 ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt caactctcag
    14521 cggctcatcc agccacaaac gccagccggt aatattggct gccggataac gcatcagacg
    14581 cgatgcatcc tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa
    14641 agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct
    14701 ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact gtgaggcaag
    14761 ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac
    14821 cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc
    14881 cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac
    14941 gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga
    15001 agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc
    15061 gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga
    15121 aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt aacgcaggcc
    15181 aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga
    15241 gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat
    15301 ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc
    15361 ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata
    15421 accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat gaaacaaaaa
    15481 gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg
    15541 gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga agtcctgagc
    15601 aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt
    15661 atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat
    15721 aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg
    15781 ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc
    15841 gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg
    15901 tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg
    15961 ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg
    16021 tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg cgcatggttt
    16081 ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct
    16141 tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg
    16201 ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg attcggtatg
    16261 cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat
    16321 cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg
    16381 tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt
    16441 actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc
    16501 tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac cccagccggg
    16561 gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga
    16621 tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca
    16681 atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg
    16741 cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact
    16801 cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga
    16861 tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca
    16921 tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg
    16981 catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca
    17041 cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca
    17101 actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac
    17161 gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt
    17221 ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga
    17281 ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat
    17341 tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat
    17401 ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc agccattgtt
    17461 cttcagcgaa ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa
    17521 tactggcgat ctggaaacca gtgccgaacg tttccaagct gacacgctgg cgcgttttga
    17581 gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt
    17641 ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa ctgaacattt
    17701 accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca
    17761 ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc
    17821 ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg
    17881 aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta
    17941 tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc tggcgctgat
    18001 ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac
    18061 catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt
    18121 ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag ctggcggcat
    18181 tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc
    18241 gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa
    18301 acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt
    18361 ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa
    18421 acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa ccacgccgga
    18481 tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg
    18541 tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct
    18601 ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc ttgcgcagca
    18661 gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc
    18721 ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat
    18781 cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg
    18841 cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc
    18901 gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa
    18961 tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct
    19021 ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg
    19081 tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa
    19141 agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg gtcacgggca
    19201 gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt
    19261 gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat
    19321 cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg
    19381 acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag cgatgactac
    19441 cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc
    19501 gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag
    19561 cggttcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga
    19621 tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt
    19681 cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga acacgcgtct
    19741 gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt
    19801 cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc tggatattgc
    19861 ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg
    19921 cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct
    19981 gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga
    20041 tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga
    20101 gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc aacatttaca
    20161 aattctttgc actttcctgc actatccgac actgtcacca tccataattc aggcttatct
    20221 gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa acgcgttttt
    20281 ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag
    20341 ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac aaatttcatg
    20401 tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac gtcggctttc
    20461 tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt
    20521 taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca
    20581 ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa
    20641 taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc accgtttcag
    20701 acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt tataaagcac
    20761 agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg ggccatcatg
    20821 cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac gccgatttcg
    20881 gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat atcaaaacac
    20941 tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg ataaaagtct
    21001 ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca gagaagattg
    21061 atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca tttaaagatg
    21121 cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg
    21181 tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat aaaaaaaatc
    21241 cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa tgagggttaa
    21301 tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta cagaagtaat
    21361 acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt ccagcgatcc
    21421 cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt ctggcgttga
    21481 ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg ccgcagcgat
    21541 gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagaaatt
    21601 acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag atatttttta
    21661 taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa gcaattattt
    21721 ttatgggatc tggactggtg aacggaggtg attattttta taataacctt tcattcacag
    21781 tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg
    21841 gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc
    21901 aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga gccaaagcgc
    21961 ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct
    22021 atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg
    22081 agtctgcgct ggctgactat tttgcggcca tcgccaactg ctttaccagc aaagacaccc
    22141 cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatag
    22201 ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc
    22261 aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc
    22321 ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac
    22381 tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa
    22441 aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac
    22501 tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg
    22561 gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat
    22621 tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct
    22681 ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac
    22741 agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc caccagcaac
    22801 agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac
    22861 tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga
    22921 aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca ctgccgctac
    22981 gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac
    23041 acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca
    23101 gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag tctgaaggaa
    23161 tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg
    23221 cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg
    23281 gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat
    23341 gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt
    23401 caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg
    23461 acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc ttaccgttca
    23521 tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg
    23581 gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt
    23641 ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt
    23701 caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca
    23761 gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga
    23821 ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagtag
    23881 agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca
    23941 gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc gcgttgtgca
    24001 attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc
    24061 catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc tggtggaagc
    24121 gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga
    24181 aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt ttacgttccg
    24241 gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac
    24301 gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc gcatgggcca
    24361 gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagtg
    24421 gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttacgtc
    24481 ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca
    24541 caatcttttt caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat
    24601 taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg
    24661 ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta atcaagtttt
    24721 gttgcttaaa agataggtaa ctttaccggg ggagatcaaa aaatagagtt tattgtttat
    24781 tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg cgtttggtcg
    24841 tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg
    24901 gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg ttacctgatg
    24961 cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat
    25021 attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcaactc
    25081 ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa ccagacaacg
    25141 atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg catactcttc
    25201 cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac
    25261 ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca ggccaacgcc
    25321 gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt ctttaagcag
    25381 atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc
    25441 aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg
    25501 taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct
    25561 gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca
    25621 tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga
    25681 tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag accaggatca
    25741 tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg
    25801 caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca
    25861 aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga
    25921 tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg
    25981 acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg
    26041 agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt
    26101 catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat
    26161 aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc gcaaagctga
    26221 tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct tgcgccagtt
    26281 tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt aagcgggtaa
    26341 aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat
    26401 tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga tcatgatagt
    26461 tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca cgctcttccg
    26521 cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt
    26581 tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt
    26641 tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg aaaccatcgc
    26701 ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc
    26761 aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag cgcgatatca
    26821 ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg cactaaattt
    26881 ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc ccgcgctttg
    26941 cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc gctttgctgc
    27001 cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt
    27061 agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt taccgcgccg
    27121 ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt
    27181 gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga
    27241 tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actaccgcca
    27301 ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat
    27361 tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc
    27421 gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc
    27481 gctggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg
    27541 ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagttgctg
    27601 ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc
    27661 ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct
    27721 acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt
    27781 ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg
    27841 gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc
    27901 ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac
    27961 acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat
    28021 caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg
    28081 gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc
    28141 agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg
    28201 ggttcttgcc tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa
    28261 aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat
    28321 tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacacc
    28381 agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg
    28441 gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat
    28501 ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa
    28561 tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt
    28621 tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa
    28681 tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac
    28741 gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat
    28801 gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct
    28861 gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat
    28921 cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac
    28981 acgacaaata tgaatgcgta tatttctcat ttgcatttgc atttgcagca aaattattcg
    29041 cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac aacataaacc
    29101 aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca agtcatcacc
    29161 aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct
    29221 gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc
    29281 gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaacgt
    29341 gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa
    29401 acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat
    29461 cgtgctcttt attattcccg cggattccag atcgataact atatggttga tggtatcccc
    29521 acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actgtttgaa
    29581 cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg
    29641 gcaattaata tggttcgaaa acacgcgacc tgtcgtgaat ttaaaggcga tgtctcggcg
    29701 gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa
    29761 gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac
    29821 cgctacaata gtgaaaagac cttcttctcg ggcattgtcg atgctgattt aggcgatctt
    29881 acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctggggc
    29941 ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc acgcagtacc
    30001 gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag
    30061 cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt cgaatttgac
    30121 agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct ggttgggcca
    30181 tacagtaatt atggacctgg ctttgatcat gtcggcggca ccggttggaa cagtggcaaa
    30241 cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt tggtcgtcag
    30301 cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttcagt tcatgggcca
    30361 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaagccg
    30421 actggtcatc acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg
    30481 ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
    30541 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
    30601 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
    30661 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
    30721 agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc
    30781 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
    30841 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
    30901 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt gcagaggata
    30961 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
    31021 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg caaaatcgcg
    31081 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
    31141 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
    31201 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
    31261 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
    31321 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
    31381 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaaccag
    31441 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
    31501 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
    31561 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
    31621 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
    31681 tggtgtagta aacaatcgca taaccgatac cgacgatcgg taacagccac agtttgctgc
    31741 tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca
    31801 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
    31861 ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg gtcaggaacg
    31921 aggtcagcgc cgcggagatc ataataccgc ccactttcgc acggttttct ggtttagcag
    31981 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
    32041 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
    32101 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
    32161 gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac gcaactaccg
    32221 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
    32281 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
    32341 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
    32401 tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaagtgtt
    32461 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
    32521 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
    32581 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
    32641 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
    32701 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
    32761 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tatgggtgct
    32821 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
    32881 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
    32941 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
    33001 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
    33061 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
    33121 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
    33181 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
    33241 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
    33301 cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg ccggaaaagg
    33361 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
    33421 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
    33481 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
    33541 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
    33601 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
    33661 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
    33721 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
    33781 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
    33841 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
    33901 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
    33961 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
    34021 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
    34081 agtcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc
    34141 atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
    34201 agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca
    34261 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt
    34321 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaagtc
    34381 acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
    34441 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
    34501 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
    34561 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
    34621 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
    34681 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
    34741 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
    34801 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
    34861 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
    34921 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
    34981 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
    35041 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
    35101 caacttccgg ggtaacatcg agatagagcg ttaagtctgg gcgaaaatcc ccgagaacag
    35161 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
    35221 gagtggaaag atcgtggcga tcgccaatca cccaggtgcc gttagccagc gcaggtttga
    35281 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
    35341 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
    35401 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg
    35461 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
    35521 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
    35581 tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
    35641 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
    35701 gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
    35761 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
    35821 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
    35881 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct
    35941 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
    36001 ttgtgaactc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat caaggtgtct
    36061 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
    36121 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
    36181 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
    36241 tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
    36301 ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg
    36361 atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg
    36421 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
    36481 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
    36541 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
    36601 acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
    36661 taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg acaagctgat
    36721 aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
    36781 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
    36841 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
    36901 gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatgag
    36961 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
    37021 cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg
    37081 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
    37141 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca caggaaatca
    37201 tcaaccgctg acaagcatcc tgtagtcgca ggatatgcgc cgataacaaa ctgactttac
    37261 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
    37321 ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa
    37381 ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg
    37441 cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt taaagatcaa
    37501 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
    37561 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt
    37621 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc
    37681 acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
    37741 tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt
    37801 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
    37861 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
    37921 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
    37981 cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc
    38041 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
    38101 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
    38161 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa tgttgtgcac
    38221 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
    38281 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
    38341 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
    38401 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
    38461 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
    38521 ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag caaatttcgt
    38581 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
    38641 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
    38701 aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac
    38761 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
    38821 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
    38881 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
    38941 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
    39001 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
    39061 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
    39121 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
    39181 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
    39241 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    39301 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    39361 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    39421 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    39481 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    39541 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    39601 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    39661 ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    39721 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    39781 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca
    39841 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    39901 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    39961 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    40021 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    40081 aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    40141 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    40201 cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt
    40261 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    40321 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct
    40381 ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc
    40441 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    40501 gcaataggcg catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg
    40561 gcttcttgca tgaacttacc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    40621 acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    40681 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    40741 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    40801 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    40861 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    40921 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcagagcc ccaagtgaat
    40981 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    41041 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc
    41101 accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    41161 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    41221 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    41281 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    41341 tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc ttcagaggca
    41401 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    41461 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    41521 ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcacaggc tgctgcaacg
    41581 tcaaatgccg ggcaaccttt aatgtccaac atgctttgaa tctgacaagc tgcgctcggg
    41641 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    41701 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    41761 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    41821 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    41881 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    41941 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    42001 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    42061 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    42121 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    42181 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    42241 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    42301 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    42361 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    42421 gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta cttcttcttc
    42481 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    42541 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    42601 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    42661 ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    42721 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    42781 tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    42841 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    42901 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    42961 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    43021 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    43081 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    43141 ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg
    43201 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    43261 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    43321 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    43381 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    43441 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    43501 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    43561 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    43621 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    43681 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    43741 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    43801 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    43861 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    43921 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    43981 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    44041 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    44101 gtcgcgctct tcttgaaaaa ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg
    44161 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    44221 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    44281 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    44341 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    44401 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg
    44461 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    44521 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    44581 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    44641 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcagacggcc
    44701 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    44761 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    44821 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    44881 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    44941 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    45001 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    45061 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    45121 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc
    45181 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    45241 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    45301 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    45361 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    45421 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    45481 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    45541 agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata
    45601 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt
    45661 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    45721 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    45781 gttttgcacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    45841 cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta
    45901 taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga
    45961 aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg
    46021 tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat
    46081 atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa
    46141 aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg
    46201 cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag
    46261 tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca
    46321 gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga
    46381 aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga
    46441 accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact
    46501 aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat
    46561 taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg
    46621 caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat
    46681 gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg accgtaccga
    46741 attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta tcgtgcgcac
    46801 cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca
    46861 ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga
    46921 gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg gcgaaatcct
    46981 tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat taggtcgccc
    47041 ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca
    47101 gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc
    47161 cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg cacgcgcgac
    47221 ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg ccgatgagat
    47281 tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat
    47341 gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga
    47401 ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgcca
    47461 gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct gctccggtac
    47521 cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga
    47581 agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta
    47641 tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg
    47701 ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg
    47761 caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg aagaagcgat
    47821 ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac ctgcactggc
    47881 aacctttgcc atgccggatg taccgccagc gccaacccca gctgaacctg ccgcgactgt
    47941 cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac aacctgggct
    48001 gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag aagccaaacc
    48061 gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa
    48121 acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac
    48181 tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac
    48241 tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga
    48301 cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc
    48361 gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga
    48421 agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg
    48481 ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc
    48541 tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct
    48601 gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga
    48661 caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca
    48721 gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt
    48781 agcgtgcacg tctccggaat tggcctctgg caaagtctgg atccgctatc caattgtacg
    48841 tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat
    48901 ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga
    48961 agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt
    49021 tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac
    49081 cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc
    49141 gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga
    49201 acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga
    49261 aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat
    49321 gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg
    49381 ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc
    49441 ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc
    49501 cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa
    49561 atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc
    49621 cagagcgggc agacaaaaca tacccagttt catgatatgt tttaaagcga gcttatttgt
    49681 tgagctggaa gagcgacaat ccctgcatat cggtaaatgc tttatacgat gcctgcaatg
    49741 ccgtttgctg catgatgtaa gatgaaatag ttgcattcca gtccacatca accagatcgc
    49801 tcatctgctg cgtttgccct aaagcgcgat cgctacctaa ggaatccagc gactccagtt
    49861 tgttcagctg cgtgcctaat tccgcgcgaa cggtaaggac attattcagc gagtttttca
    49921 gtccacggtt ggttttatcc aatgccgcag cggcgatttc tttatccgct tcgctatccg
    49981 cgaccggcgt tttcagggct gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag
    50041 aagcgctacc gtctggttcc gctaccgcgt tactggtaat actgtcgaaa attttgtcgc
    50101 ccgtatgtcc tatcaccatc gaacgcgaag catcgacctg ttgcttaata ctttctgttc
    50161 cgccaacata atcaccgtta gcctcactaa acggcgcagt ctctgtttta taaccggcaa
    50221 aaatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    50281 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    50341 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    50401 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    50461 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    50521 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    50581 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    50641 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    50701 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    50761 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    50821 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    50881 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    50941 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    51001 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    51061 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    51121 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    51181 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    51241 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    51301 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    51361 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    51421 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    51481 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    51541 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    51601 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    51661 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    51721 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    51781 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    51841 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    51901 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    51961 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    52021 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    52081 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    52141 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    52201 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    52261 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    52321 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    52381 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    52441 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    52501 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    52561 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    52621 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    52681 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    52741 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    52801 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    52861 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    52921 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    52981 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    53041 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    53101 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    53161 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    53221 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    53281 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    53341 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    53401 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    53461 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    53521 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    53581 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    53641 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    53701 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    53761 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    53821 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    53881 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    53941 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    54001 gcccttaagc ggtgtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    54061 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    54121 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    54181 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    54241 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    54301 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    54361 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    54421 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    54481 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    54541 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    54601 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    54661 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    54721 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    54781 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    54841 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    54901 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    54961 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    55021 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    55081 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    55141 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    55201 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    55261 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct
    55321 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    55381 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    55441 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    55501 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    55561 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    55621 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    55681 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    55741 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    55801 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    55861 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    55921 actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc acgcgacagt
    55981 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    56041 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    56101 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    56161 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    56221 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    56281 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    56341 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    56401 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    56461 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    56521 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    56581 cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    56641 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    56701 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    56761 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    56821 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    56881 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    56941 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagtttccc
    57001 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    57061 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    57121 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    57181 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggta
    57241 ttttgctgca tggagttgag gaagctcaga ctaaacgtgg cgggttctgc accgttaatt
    57301 gcgccggttg cgatattatt cgccatcgca ccatccacta atgtgccatt agcattaaat
    57361 tccagtgttg tcgctgtctt cgcaatgctg tttggatcac tgctatcctg ggtgtagacc
    57421 tgccagttat tatccccggt cttcacaaaa tagacgctca tgtcatgagc attaccctga
    57481 ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat
    57541 ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga cgccgtggtg
    57601 gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc
    57661 tgaatagtcg gcggcgtacc ggttaccggg taacccgtca gctgtaaacc ttgcgtattc
    57721 aacaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg
    57781 ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg
    57841 gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt tacccccagt
    57901 cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag
    57961 gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat
    58021 ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc
    58081 tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg
    58141 ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta
    58201 ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccatca
    58261 gtcaacgaac cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg
    58321 gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt
    58381 tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc
    58441 gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac
    58501 gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag tttttcaatc
    58561 ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg
    58621 gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct
    58681 gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga
    58741 tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt
    58801 tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg
    58861 ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt acgtagccct
    58921 ttgcatctgc cagcggatta cctggttcat aaaccagttt gtccggggcc tgactttctt
    58981 aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac
    59041 cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag catttgccag
    59101 attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc
    59161 aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt
    59221 gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg
    59281 ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg
    59341 gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt
    59401 catcgtcagt gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc
    59461 actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc
    59521 tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc
    59581 ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa
    59641 atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt
    59701 tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct
    59761 gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtacggcgag
    59821 caatctcaca tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg taagtgatga
    59881 ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccatgcgagc agccattgct
    59941 ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa
    60001 cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat
    60061 gcctattgtg cggggaggaa agctggaagc tggcaatgtc aaactgaaac gtggacgcct
    60121 ggataccctg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct
    60181 gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt
    60241 aaaagcggga caacgcgtca atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga
    60301 aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg
    60361 acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt
    60421 taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga
    60481 ggaataaacc atgagtattg atcgcacttc gcctctgaag cctgtaagca ccgttcaacc
    60541 gcgcgaaacc accgacgcgc cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac
    60601 cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga
    60661 tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat
    60721 ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa
    60781 ctgatcgtat gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc
    60841 tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc
    60901 agttgcaatg gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac
    60961 agttacgcaa gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg
    61021 aaattactgt gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg
    61081 aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga
    61141 cgctatatgg ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga
    61201 tctgaatatt atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg
    61261 gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc
    61321 gccagcacga ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc
    61381 ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga
    61441 cgcaacagaa acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc
    61501 cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca
    61561 tgtttcaacg gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg
    61621 gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc
    61681 actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc
    61741 gcatcaaacg cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga
    61801 atgcccaacg caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc
    61861 atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc
    61921 aaaatggtgc caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg
    61981 taatacatcc aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt
    62041 aaggagatct ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc
    62101 attgctccag catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg
    62161 tgcggtagct gatacaccag ctgcaggatg ccgccgaccg taacagccca cgccagcgcc
    62221 agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta
    62281 agcagtgttg gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc
    62341 accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg
    62401 gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac
    62461 ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa
    62521 acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga
    62581 atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga
    62641 agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca
    62701 attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc
    62761 agcgatttta ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag
    62821 gcaggcgctg aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg
    62881 ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag
    62941 cactgcctat tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt caatgaagtg
    63001 ccgcgcacag ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt
    63061 attcactaca ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag
    63121 cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca
    63181 agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc
    63241 gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc
    63301 ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac
    63361 gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa
    63421 ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg
    63481 cgccgccagt tccaccagcc tttcagcatc gcgcagattt tctgccagcg gtttatcgac
    63541 acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct
    63601 ggagtgcaca aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg
    63661 aatgcgccag ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg
    63721 taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc
    63781 taatcccact acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg
    63841 aatcaagacg ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctata
    63901 aatcaccgtc aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac
    63961 tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa
    64021 aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc
    64081 tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca
    64141 ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg
    64201 aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta
    64261 gataatgacg tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca
    64321 tgaccggttt acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg
    64381 ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca
    64441 aggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta
    64501 gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca
    64561 tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc
    64621 atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg
    64681 gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga
    64741 tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct
    64801 ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta
    64861 atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc
    64921 tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca
    64981 cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta
    65041 ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag
    65101 tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta
    65161 ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc
    65221 cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc
    65281 ccgggagggg aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc
    65341 atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc
    65401 ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag
    65461 tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa
    65521 ccgatgattg ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc
    65581 cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt
    65641 gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt
    65701 ttctcgctgt tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc
    65761 tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta
    65821 tgtgcggcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc
    65881 gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg
    65941 ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac
    66001 gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg
    66061 ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg
    66121 ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc
    66181 ctacaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct
    66241 gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg
    66301 tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg
    66361 tttgacctgg gcaaatcggc gcaccagcca gagcttccgt ggatgatgct gggaattatt
    66421 ggcatcttca ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt
    66481 ttgctggaac gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa
    66541 ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc
    66601 ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc
    66661 aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa
    66721 aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc
    66781 gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag
    66841 gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag
    66901 ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat
    66961 tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt
    67021 gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat
    67081 attcagctat ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc
    67141 cgcgttgcta attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca
    67201 atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca
    67261 gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga
    67321 cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt
    67381 cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat
    67441 taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc
    67501 tgacgcccat attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa
    67561 ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc
    67621 gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt
    67681 cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat
    67741 gcaaacgttg cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc
    67801 ttacgtcctg cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg
    67861 cattgaagtg aagccaggcg aaattgtcat tccgtttacg gattaatgac gagggcgcat
    67921 tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa
    67981 aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat
    68041 ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt
    68101 gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt
    68161 gaccacagtt gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg
    68221 gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct
    68281 ggagcgcgga tttaatgaag ccgtgttcac cgctgcaaaa ctttacccgg caaacgcaac
    68341 cactaactcc agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat
    68401 ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat
    68461 ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac
    68521 tgcgctgaaa gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga
    68581 cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca
    68641 tatgctggtt ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat
    68701 tcaccaacag gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac
    68761 ggattctgcg ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt
    68821 caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca
    68881 gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga
    68941 cacattcatc gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga
    69001 tgacaccctg gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa
    69061 atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag tatgttcaac
    69121 agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg
    69181 ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg
    69241 aaggccacgt atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa
    69301 aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact
    69361 ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cggatcgccc cggctttttt
    69421 attttactta cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata
    69481 tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa
    69541 tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt
    69601 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
    69661 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
    69721 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
    69781 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
    69841 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
    69901 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
    69961 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
    70021 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
    70081 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
    70141 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
    70201 gaaggcgaaa agtatgtccc gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc
    70261 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct
    70321 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
    70381 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
    70441 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
    70501 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
    70561 cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa
    70621 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
    70681 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
    70741 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
    70801 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
    70861 gttccgtttg cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta
    70921 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
    70981 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
    71041 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
    71101 gaccaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
    71161 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
    71221 atctcatcaa taatattcgc gagcatttga tgctttatct ttttctctgg ggattgttgg
    71281 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
    71341 aatatttaat ctccctttca tttagcgtga aaaattgaat gatattattc ctgcttctac
    71401 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
    71461 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
    71521 ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc
    71581 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
    71641 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
    71701 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
    71761 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
    71821 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt
    71881 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
    71941 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
    72001 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat
    72061 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
    72121 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
    72181 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
    72241 gcacgccttt gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt
    72301 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa
    72361 tgtgacaatt aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg
    72421 cagtgcggat tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt
    72481 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
    72541 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg
    72601 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
    72661 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
    72721 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
    72781 aatacccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
    72841 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
    72901 ctcactacaa caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg
    72961 gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg
    73021 cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
    73081 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
    73141 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
    73201 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
    73261 gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa
    73321 gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc
    73381 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
    73441 aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa
    73501 ggatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa
    73561 ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
    73621 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata
    73681 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
    73741 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
    73801 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
    73861 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
    73921 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
    73981 gggcgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
    74041 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
    74101 tgggtattgg cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg
    74161 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
    74221 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
    74281 agaatttcga atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg
    74341 accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
    74401 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc
    74461 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
    74521 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
    74581 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc
    74641 atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga
    74701 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
    74761 cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccacca gagtgttctc
    74821 cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
    74881 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
    74941 gcgttccttc acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta
    75001 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
    75061 gggcgcataa ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac
    75121 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
    75181 tcgtcgcctg gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct
    75241 tacccctcta cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg
    75301 gtattgtctt cagtataatg ggttcctaaa cacatcattt taggtattga atatgagacg
    75361 ggcaatcagc tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg
    75421 acaatctaag ctggttactg actggcttga taagaaccct gagttttact tggattcatc
    75481 cttgagcttt aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct
    75541 aggtgacttt ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat
    75601 cgagagttta gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacgccg
    75661 tatcttaagg gctggtgtag atgtagtcac actgagcgat ggagaacatt acacacgaga
    75721 aagtttaaaa gacccgttag ccctgattaa aagcattctg ataatgcagc gggcgcatga
    75781 ggaaagtttg agaaagtctg agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat
    75841 ctccgaaggt atcaaggtat
//
LOCUS       DAJTNV010000002        75617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNV010000002 DAJTNV010000000
VERSION     DAJTNV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(436..642)
                     /locus_tag="OO199_000083"
     CDS             complement(436..642)
                     /locus_tag="OO199_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5534285.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            955..1044
                     /gene="kdpF"
                     /locus_tag="OO199_000084"
     CDS             955..1044
                     /gene="kdpF"
                     /locus_tag="OO199_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5534286.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1044..2717
                     /gene="kdpA"
                     /locus_tag="OO199_000085"
     CDS             1044..2717
                     /gene="kdpA"
                     /locus_tag="OO199_000085"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5534287.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2740..4787
                     /gene="kdpB"
                     /locus_tag="OO199_000086"
                     /pseudo
     CDS             2740..4787
                     /gene="kdpB"
                     /locus_tag="OO199_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4796..5368
                     /gene="kdpC"
                     /locus_tag="OO199_000087"
     CDS             4796..5368
                     /gene="kdpC"
                     /locus_tag="OO199_000087"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5534288.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..8041
                     /gene="kdpD"
                     /locus_tag="OO199_000088"
                     /pseudo
     CDS             5361..8041
                     /gene="kdpD"
                     /locus_tag="OO199_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8038..8715
                     /gene="kdpE"
                     /locus_tag="OO199_000089"
     CDS             8038..8715
                     /gene="kdpE"
                     /locus_tag="OO199_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5534289.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8990..9094
                     /locus_tag="OO199_000090"
     CDS             8990..9094
                     /locus_tag="OO199_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5534290.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9405..11602
                     /gene="speF"
                     /locus_tag="OO199_000091"
                     /pseudo
     CDS             9405..11602
                     /gene="speF"
                     /locus_tag="OO199_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11599..12918
                     /gene="potE"
                     /locus_tag="OO199_000092"
     CDS             11599..12918
                     /gene="potE"
                     /locus_tag="OO199_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5534291.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12974..14614)
                     /gene="pgm"
                     /locus_tag="OO199_000093"
     CDS             complement(12974..14614)
                     /gene="pgm"
                     /locus_tag="OO199_000093"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5534292.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14640..15185)
                     /gene="seqA"
                     /locus_tag="OO199_000094"
     CDS             complement(14640..15185)
                     /gene="seqA"
                     /locus_tag="OO199_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5534293.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15370..16134
                     /gene="ybfF"
                     /locus_tag="OO199_000095"
     CDS             15370..16134
                     /gene="ybfF"
                     /locus_tag="OO199_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5534294.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16274..16567
                     /gene="ybfE"
                     /locus_tag="OO199_000096"
     CDS             16274..16567
                     /gene="ybfE"
                     /locus_tag="OO199_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5534295.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16707..17237
                     /gene="fldA"
                     /locus_tag="OO199_000097"
     CDS             16707..17237
                     /gene="fldA"
                     /locus_tag="OO199_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5534296.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17447..17533
                     /gene="uof"
                     /locus_tag="OO199_000098"
     CDS             17447..17533
                     /gene="uof"
                     /locus_tag="OO199_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5534297.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17526..17972
                     /gene="fur"
                     /locus_tag="OO199_000099"
     CDS             17526..17972
                     /gene="fur"
                     /locus_tag="OO199_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5534298.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18056..18382)
                     /locus_tag="OO199_000100"
     CDS             complement(18056..18382)
                     /locus_tag="OO199_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534299.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18419..19825)
                     /gene="chiP"
                     /locus_tag="OO199_000101"
     CDS             complement(18419..19825)
                     /gene="chiP"
                     /locus_tag="OO199_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5534300.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20404..22068)
                     /gene="glnS"
                     /locus_tag="OO199_000102"
     CDS             complement(20404..22068)
                     /gene="glnS"
                     /locus_tag="OO199_000102"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5534301.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22271..24217)
                     /gene="nagE"
                     /locus_tag="OO199_000103"
     CDS             complement(22271..24217)
                     /gene="nagE"
                     /locus_tag="OO199_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5534302.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24550..25350
                     /gene="nagB"
                     /locus_tag="OO199_000104"
     CDS             24550..25350
                     /gene="nagB"
                     /locus_tag="OO199_000104"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5534303.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25410..26558
                     /gene="nagA"
                     /locus_tag="OO199_000105"
     CDS             25410..26558
                     /gene="nagA"
                     /locus_tag="OO199_000105"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5534304.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26567..27787
                     /gene="nagC"
                     /locus_tag="OO199_000106"
                     /gene_synonym="nagR"
     CDS             26567..27787
                     /gene="nagC"
                     /locus_tag="OO199_000106"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5534305.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27835..28587
                     /gene="nagD"
                     /locus_tag="OO199_000107"
     CDS             27835..28587
                     /gene="nagD"
                     /locus_tag="OO199_000107"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5534306.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28984..30648
                     /gene="asnB"
                     /locus_tag="OO199_000108"
     CDS             28984..30648
                     /gene="asnB"
                     /locus_tag="OO199_000108"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5534307.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31027..31103
                     /locus_tag="OO199_000109"
     tRNA            31027..31103
                     /locus_tag="OO199_000109"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31061..31063,aa:Met,seq:cat)
     gene            31112..31196
                     /locus_tag="OO199_000110"
     tRNA            31112..31196
                     /locus_tag="OO199_000110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31146..31148,aa:Leu,seq:tag)
     gene            31220..31294
                     /locus_tag="OO199_000111"
     tRNA            31220..31294
                     /locus_tag="OO199_000111"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31252..31254,aa:Gln,seq:ttg)
     gene            31329..31403
                     /locus_tag="OO199_000112"
     tRNA            31329..31403
                     /locus_tag="OO199_000112"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31361..31363,aa:Gln,seq:ttg)
     gene            31419..31495
                     /locus_tag="OO199_000113"
     tRNA            31419..31495
                     /locus_tag="OO199_000113"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31453..31455,aa:Met,seq:cat)
     gene            31544..31618
                     /locus_tag="OO199_000114"
     tRNA            31544..31618
                     /locus_tag="OO199_000114"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31576..31578,aa:Gln,seq:ctg)
     gene            31656..31730
                     /locus_tag="OO199_000115"
     tRNA            31656..31730
                     /locus_tag="OO199_000115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31688..31690,aa:Gln,seq:ctg)
     gene            complement(31884..33059)
                     /gene="ubiF"
                     /locus_tag="OO199_000116"
     CDS             complement(31884..33059)
                     /gene="ubiF"
                     /locus_tag="OO199_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5534308.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33205..34629
                     /gene="miaB"
                     /locus_tag="OO199_000117"
     CDS             33205..34629
                     /gene="miaB"
                     /locus_tag="OO199_000117"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5534309.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34782..35822
                     /locus_tag="OO199_000118"
     CDS             34782..35822
                     /locus_tag="OO199_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5534310.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35819..36286
                     /gene="ybeY"
                     /locus_tag="OO199_000119"
     CDS             35819..36286
                     /gene="ybeY"
                     /locus_tag="OO199_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5534311.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36376..37254
                     /gene="corC"
                     /locus_tag="OO199_000120"
     CDS             36376..37254
                     /gene="corC"
                     /locus_tag="OO199_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5534312.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37279..38817
                     /gene="lnt"
                     /locus_tag="OO199_000121"
     CDS             37279..38817
                     /gene="lnt"
                     /locus_tag="OO199_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5534313.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39214..40122
                     /gene="gltI"
                     /locus_tag="OO199_000122"
     CDS             39214..40122
                     /gene="gltI"
                     /locus_tag="OO199_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5534314.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40292..41032
                     /gene="gltJ"
                     /locus_tag="OO199_000123"
     CDS             40292..41032
                     /gene="gltJ"
                     /locus_tag="OO199_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5534315.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41032..41706
                     /gene="gltK"
                     /locus_tag="OO199_000124"
     CDS             41032..41706
                     /gene="gltK"
                     /locus_tag="OO199_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5534316.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41706..42431
                     /gene="gltL"
                     /locus_tag="OO199_000125"
     CDS             41706..42431
                     /gene="gltL"
                     /locus_tag="OO199_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5534317.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42549..43484
                     /gene="rihA"
                     /locus_tag="OO199_000126"
     CDS             42549..43484
                     /gene="rihA"
                     /locus_tag="OO199_000126"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5534318.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43568..45229
                     /gene="hscC"
                     /locus_tag="OO199_000127"
                     /pseudo
     CDS             43568..45229
                     /gene="hscC"
                     /locus_tag="OO199_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45289..46740)
                     /gene="djlC"
                     /locus_tag="OO199_000128"
     CDS             complement(45289..46740)
                     /gene="djlC"
                     /locus_tag="OO199_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5534319.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46737..47444)
                     /locus_tag="OO199_000129"
     CDS             complement(46737..47444)
                     /locus_tag="OO199_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5534320.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47546..48100
                     /locus_tag="OO199_000130"
     CDS             47546..48100
                     /locus_tag="OO199_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5534321.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48110..49537)
                     /locus_tag="OO199_000131"
     CDS             complement(48110..49537)
                     /locus_tag="OO199_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5534322.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49534..50241)
                     /locus_tag="OO199_000132"
     CDS             complement(49534..50241)
                     /locus_tag="OO199_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5534323.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50405..51382
                     /locus_tag="OO199_000133"
     CDS             50405..51382
                     /locus_tag="OO199_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5534324.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51452..51934)
                     /locus_tag="OO199_000134"
     CDS             complement(51452..51934)
                     /locus_tag="OO199_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5534325.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52169..54751
                     /gene="leuS"
                     /locus_tag="OO199_000135"
     CDS             52169..54751
                     /gene="leuS"
                     /locus_tag="OO199_000135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5534326.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54766..55347
                     /gene="lptE"
                     /locus_tag="OO199_000136"
     CDS             54766..55347
                     /gene="lptE"
                     /locus_tag="OO199_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5534327.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55347..56378
                     /gene="holA"
                     /locus_tag="OO199_000137"
     CDS             55347..56378
                     /gene="holA"
                     /locus_tag="OO199_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5534328.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56380..57021
                     /gene="nadD"
                     /locus_tag="OO199_000138"
     CDS             56380..57021
                     /gene="nadD"
                     /locus_tag="OO199_000138"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5534329.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNASSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57045..57656
                     /locus_tag="OO199_000139"
     CDS             57045..57656
                     /locus_tag="OO199_000139"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5534330.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57916..58233
                     /gene="rsfS"
                     /locus_tag="OO199_000140"
                     /gene_synonym="rsfA"
     CDS             57916..58233
                     /gene="rsfS"
                     /locus_tag="OO199_000140"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5534331.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58237..58704
                     /gene="rlmH"
                     /locus_tag="OO199_000141"
     CDS             58237..58704
                     /gene="rlmH"
                     /locus_tag="OO199_000141"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5534332.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58735..60636
                     /gene="mrdA"
                     /locus_tag="OO199_000142"
     CDS             58735..60636
                     /gene="mrdA"
                     /locus_tag="OO199_000142"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5534333.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60639..61751
                     /gene="mrdB"
                     /locus_tag="OO199_000143"
                     /gene_synonym="rodA"
     CDS             60639..61751
                     /gene="mrdB"
                     /locus_tag="OO199_000143"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5534334.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61762..62850
                     /gene="rlpA"
                     /locus_tag="OO199_000144"
     CDS             61762..62850
                     /gene="rlpA"
                     /locus_tag="OO199_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5534335.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62989..64200
                     /gene="dacA"
                     /locus_tag="OO199_000145"
     CDS             62989..64200
                     /gene="dacA"
                     /locus_tag="OO199_000145"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5534336.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64311..64574
                     /locus_tag="OO199_000146"
     CDS             64311..64574
                     /locus_tag="OO199_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5534337.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64675..65316
                     /gene="lipB"
                     /locus_tag="OO199_000147"
     CDS             64675..65316
                     /gene="lipB"
                     /locus_tag="OO199_000147"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5534338.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65575..66528
                     /locus_tag="OO199_000148"
     CDS             65575..66528
                     /locus_tag="OO199_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5534339.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66737..67702
                     /gene="lipA"
                     /locus_tag="OO199_000149"
     CDS             66737..67702
                     /gene="lipA"
                     /locus_tag="OO199_000149"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5534340.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67803..68006)
                     /gene="tatE"
                     /locus_tag="OO199_000150"
     CDS             complement(67803..68006)
                     /gene="tatE"
                     /locus_tag="OO199_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5534341.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68135..68923)
                     /locus_tag="OO199_000151"
     CDS             complement(68135..68923)
                     /locus_tag="OO199_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5534342.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69016..69399
                     /gene="crcB"
                     /locus_tag="OO199_000152"
     CDS             69016..69399
                     /gene="crcB"
                     /locus_tag="OO199_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5534343.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69453..69662)
                     /gene="cspE"
                     /locus_tag="OO199_000153"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69453..69662)
                     /gene="cspE"
                     /locus_tag="OO199_000153"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5534344.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69837..70397)
                     /gene="pagP"
                     /locus_tag="OO199_000154"
     CDS             complement(69837..70397)
                     /gene="pagP"
                     /locus_tag="OO199_000154"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5534345.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70985..72370
                     /gene="dcuC"
                     /locus_tag="OO199_000155"
     CDS             70985..72370
                     /gene="dcuC"
                     /locus_tag="OO199_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5534346.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72411..72752)
                     /locus_tag="OO199_000156"
                     /pseudo
     CDS             complement(72411..72752)
                     /locus_tag="OO199_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73090..73599)
                     /locus_tag="OO199_000157"
     CDS             complement(73090..73599)
                     /locus_tag="OO199_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5534347.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73762..74007
                     /locus_tag="OO199_000158"
     CDS             73762..74007
                     /locus_tag="OO199_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5534348.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73982..75613
                     /locus_tag="OO199_000159"
                     /pseudo
     CDS             73982..75613
                     /locus_tag="OO199_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18611 a        18991 c        19205 g        18810 t
ORIGIN      
        1 acataacatc ttattggatt agatatgtta acaattaatt attcttaata tatcaataaa
       61 gtatatatta ctctctttac tatctgtttt tattgcggta gcgatactaa taacaaaatt
      121 cggaatttat ttagatgctg gaattagaaa aagggatgac ggctttctca ccttaccctg
      181 aagtttcacg aaagtgtgta aggaagtatg caatctaact ggagatttct gatgatctag
      241 tcgcttagag cgacaatgaa ggtgaccgca tcctgggtag ccctatgaaa ggatactatt
      301 gaaacgtgat gctaaaacag tgatgaaatg aataatacga tggaaacgtc ttccatgtta
      361 attatcatgg aagacgttga tgttgaacac ctaaaccagc cccctgatac gaggctggct
      421 taagcgcgca ggcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac
      481 cggtttatcg ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc
      541 tttccagaaa agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg
      601 taaaaagata agccatgcag gatattctct gtagagttcc atacttttct cccccttttg
      661 tatctacccg gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg
      721 ccttttgtgt aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt
      781 acattcacac tgtaattaaa ttacacaaaa gttaaattaa tactaaccat cagttaaatc
      841 atggcctttg ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt
      901 tgcagccaga attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt
      961 gcaggcgtga tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc
     1021 ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag
     1621 gtgcgcagca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaata gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatgct taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3781 gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3841 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3901 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct
     3961 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga
     4021 gccacgctgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat
     4081 atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg
     4261 cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctacg gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggctaaagag gcgggcaata
     4381 tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca
     4561 tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag
     4801 tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcgcggttc ggcattaatc gggcaaaatt ttaccggcaa
     4981 cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt
     5101 tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac
     5161 ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc
     5221 acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa
     5341 tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc
     5401 tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctgt tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctgac
     6181 gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt
     6241 accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6301 ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga
     6361 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     6421 aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6481 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa
     6541 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     6601 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6661 tggcgtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa
     6721 tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt
     6781 gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac
     6841 tgctggcgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt
     6901 atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag
     6961 cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa
     7021 cggtaaattg cagccgttaa cacatccgca aggagttacg ccatgggacg atgccatcgc
     7081 gcagtggagt tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt
     7141 accgtaccag attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga
     7201 gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac
     7261 gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
     7321 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt
     7381 acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
     7441 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
     7501 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     7561 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc cggaaccggg
     7621 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     7681 gctactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc
     7741 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     7801 ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7861 cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat
     7921 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7981 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg
     8041 acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg
     8101 gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg
     8161 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     8221 gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc
     8281 agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag
     8341 ccgtttggta ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
     8401 accgccgcgc ccgatccgct ggtgaaattt tccggtgtta ccgtcgattt agccgcccgc
     8461 gtgattcacc ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg
     8521 gtgctgctca acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg
     8581 gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa
     8641 aaactggaac aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattgggtat
     8701 cggtttatgc tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt
     8761 aaacctgcgc cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac
     8821 gaaattacaa catttaagta acaaaacagc atctaatatg gcgatattca taattaatga
     8881 tttattatct ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca
     8941 tggatagaat aactctacct tcaactgaca cagcaagcgg taaaggtaaa tggaaaataa
     9001 taaccgctta atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa
     9061 tagttttgac tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg
     9121 tacaggtctc cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat
     9181 actgctaacc cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg
     9241 acgagtatag ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact
     9301 gatttattta tcagcggtgg agctttgctt tttttccgac gtgatcgatt tctcctttga
     9361 gaaattgagg acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa
     9421 ttgccgttag ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg
     9481 acgaaagtaa ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg
     9541 gaaaactcga taaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa
     9601 atgaagaacg tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg
     9661 aatcacggaa agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc
     9721 aactgcgccc gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt
     9781 ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt
     9841 tcgtggagta cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa
     9901 tgggcgatct gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag
     9961 tgtttaatgc cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg
    10021 ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat
    10081 ctaaccacca cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac
    10141 gtaacccgta tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc
    10201 gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg
    10261 ctgtgattca gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata
    10321 aaattggtca tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat
    10381 ttatcccgat gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg
    10441 gtattctagt tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa
    10501 tccataaaaa agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga
    10561 acaacgcctt tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg
    10621 atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg
    10681 taaatggcat taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg
    10741 tacctgaact ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg
    10801 atctgcgctt cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag
    10861 agaatcaata ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac
    10921 gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg
    10981 aaaatggcgt agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg
    11041 cagaagatat ggccaaactt cagcaacttg tgccctgctg gtacgcttcg aaaaactgct
    11101 tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc atgaagagcg
    11161 ctacgccggt tataccctgc gtcagttgtg ccaggaaatg catgatttgt atgcccgcca
    11221 caacgtgaaa caactgcaaa aagagatgtt ccgcaaagcg cacttcccac acgtcagcat
    11281 gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc
    11341 ggatgccgaa ggtcgtatcg ctgccgaagg ggcgcttcct tatcctccgg gtgtgctgtg
    11401 tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt tacttcagcg ctctggaaga
    11461 agggatcaac ctgctgccag gttttgcacc ggagctgcag ggtgtctata tcgaagaaca
    11521 tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct
    11581 gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta
    11641 accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt
    11701 gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg ctcaatggca
    11761 ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg tatgggcggt
    11821 tatgccgagt atgctttcgg taaatccggt aactttattg cgaactatac ctacggcgtc
    11881 tcactgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg
    11941 ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt gctgtggatt
    12001 tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattaccgtg
    12061 tgggggggca ttattccggt ggtaggcctg tgcattatcg gctggttctg gtttagcccg
    12121 acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc agtaggttct
    12181 tccatcgcta tgacgctgtg ggcttttctt ggtctggagt ctgcgtgtgc gaatactgat
    12241 gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac gttaggtgcg
    12301 gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa tatggagctg
    12361 gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgtttacacc agaagtgggt
    12421 aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc
    12481 accattgccc aggtgtttaa atcttcatct gatgaaggct acttccctaa aattttctcc
    12541 cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat tatccagagc
    12601 gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt gctggttaac
    12661 ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt
    12721 cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt
    12781 ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat gctgtacggt
    12841 tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg ctttgaactg
    12901 aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac cgctcttttt
    12961 ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct
    13021 caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta tatgcgtctt
    13081 ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccaa
    13141 tagaagcacc gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca cctgccaggg
    13201 tgctggcgct caccatttcc ggagacagct tagacagcgc cgctttttgt gcggaagtcg
    13261 cagctgcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt tcgttgtagt
    13321 gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga
    13381 tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc
    13441 tctcttcgcc gccaaagccg aagctgccgt cgaacagacc atcaacaaac catttgaaac
    13501 ctaccggtac ttctaccagc ttacggccca agtcattgac cacacggtcg atcatcgcag
    13561 aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca
    13621 ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga
    13681 taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg
    13741 ccagcagacc cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat
    13801 cgaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt
    13861 tgtaatactc gccaatgcgc ttccagtatt cgataccgga accgcccaac ggatcaacgc
    13921 ccagcgtcag gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca
    13981 cgaacggctg caccagatcc tgctctttca catagccgga tgccatcgct tcgtcgaggg
    14041 agatacgctt cacgcctttc aggccatcgg ccatcagtgc gttggccctg tcttccacca
    14101 ctttggtgac gttagtatca gccgggccac catttggcgg attgtacttg ataccaccat
    14161 cttccggcgg gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt
    14221 tattgtgaac caggatggca ttggaaacgg caggcgtcgg agtgaagcca ttgttttcct
    14281 gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct
    14341 cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac
    14401 gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg
    14461 ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt
    14521 ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt
    14581 gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt
    14641 tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg
    14701 tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgtg
    14761 cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc
    14821 gcaaagtaca cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg
    14881 tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct
    14941 gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg
    15001 accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca
    15061 gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg
    15121 ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt
    15181 ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaagagt atatgaataa
    15241 atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt
    15301 gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag
    15361 aactttgcca tgaaattaaa tatccgcgcg caaactgcac aaaaccagca caataattct
    15421 cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt actggctcgc
    15481 gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg
    15541 agagagccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca
    15601 ctgcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc ggtaatggca
    15661 cttactgcac tggcccccga tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc
    15721 gactatcacg tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg
    15781 gacgcacaaa ctcgccagca agcagcagca ataatgcgcc agcatcttaa tgaagaaggg
    15841 gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa cgtgccggta
    15901 ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcaccct
    15961 gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta
    16021 ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca ctgggtccat
    16081 gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac
    16141 tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc
    16201 gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg tttcccccga
    16261 agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca
    16321 cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat
    16381 taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa
    16441 gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gaatcgcgta atatgagccg
    16501 tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat
    16561 cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc
    16621 aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca
    16681 ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca
    16741 ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg
    16801 atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttacgac attctgctgc
    16861 tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga
    16921 ctctcgaaga gattgatttc aatggtaaac tggttgcgct gtttggttgt ggtgaccagg
    16981 aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc attgaaccgc
    17041 gcggtgcaac catcgttggt cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag
    17101 gtctggcaga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac
    17161 tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg catctcgacg
    17221 aaattcttaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata
    17281 atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc
    17341 ccagtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat
    17401 atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat
    17461 ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat
    17521 tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc
    17581 gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt
    17641 tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac
    17701 tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg
    17761 tatttgaact gacacagcaa catcaccacg atcacctgat ttgcctcgac tgcggcaagg
    17821 ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg
    17881 gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc
    17941 gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg
    18001 cgggttggct tttttataca agggaaataa gaaaagcgag agttacagct ctcacttatt
    18061 tgttattact gcgtatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat
    18121 ttccggtttg cgttgcacgc agacactgct gataatccag tacgcgtaca ctttcctggt
    18181 cggcaaactg ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt
    18241 caattttttc tggcgaacct tgcgcggtgt tgatacaggc gctgtatgcc tctttcagac
    18301 ggctgtcttc ctgtggtgcg gttgattgcg cacaagctac cagccccgat gccatcatgg
    18361 cgatgagaat cagttttttc attgaatgcg gcaggcacaa aacctgccgc gtcgggcatc
    18421 agaagatggt gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt
    18481 taccgtaacc gccgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca
    18541 gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt
    18601 aggcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg
    18661 tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat
    18721 tccagttttt caggtcatac atcgcactga agaagaccgc tttttcgccg ttagcgttga
    18781 agtcggaacg gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttgggg
    18841 tcatacgttg caggaagtat ccctgctgac tgtcagcctt aatccaggtg ccttcgaggc
    18901 gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc
    18961 cgtcataaag atcgttgacg ctgcgatcgt caactttatc gcgggtacca tagaactggt
    19021 agctggtggt taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt
    19081 gatcgatata cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt
    19141 cgtatttcgc accgatggag tgcagataat caactttggt ggttttatcg ttctgataaa
    19201 actcatccat ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac
    19261 tcaaggcacc agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac
    19321 ccggcataaa gctccagtgc ggcgctaaca gcgtttgacc ggttggctga atgtaacctg
    19381 ccctcgccca aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc
    19441 ttttgtcgcc ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt
    19501 tcgggtggga gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa
    19561 ggccgaacat atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt
    19621 gagaaaggtt ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct
    19681 gccagtaata gataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct
    19741 ccggcattgc cataaagccc gacattgctg taacaccggc aatagccagc gccagcgtac
    19801 tacgtttgcc actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc
    19861 aaaggtgaac agcgaaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg
    19921 actacaggaa cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat
    19981 gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgcg
    20041 gttgaaaggc ttgcagaaga ataaaaacgt gaaattccag caagatgaaa gcggttacat
    20101 tttatttaat tatgttttgt gattttaaat ttattttatg gataaattgc gaagcggaat
    20161 taattcgcga agaaaagcag aaaaaacgcc gcagagcgcg gcgtttggga ttgtttgcta
    20221 tttcttgccg gatgcggcgt gaacgcctta tccggcctac aaaagcacgc aaattcaata
    20281 tattgcagag atcatgtagg cctgataagc gaagcgcatc aggcaattta gcgtttgaaa
    20341 tttattgtct atttattgcc ggatgcgacg taaatgcctc acccggcttg gtagaactta
    20401 aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt taaataccgg
    20461 tttttcggcc gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct caaactggaa
    20521 tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt tgatcaccag
    20581 cgattccggg ttaatcaccg acaggaaatc atccgcagca cctgggttag gcacgctgaa
    20641 cagacgatca tacaaacgga tttcaaccgg cagcgcatgt gccgcgctca cccagtgaat
    20701 aacacctttg actttacgac catctgccgg atctttgctt aaggtgtcgg cgtcataagt
    20761 acagaagatg gtggtgatat taccttcggc atctttctcc acgcgttcag ccttaatgac
    20821 gtaagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact gcttgttagc
    20881 ttcttcgcgg aaatcggcgc gatcgatcca aatttcaccg ctaaacggca cctgacggct
    20941 ccccatttcc ggtttgttcg gatggttcgg catggtgacc atttcgcctt cgccctgata
    21001 gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg cattttcgtt
    21061 gagatcttca cggatgcagg attccagcga cgccatctca atggtgttgt cctgcttggt
    21121 cacgccgatg cgtttgcaga actcacgaat agaagccgca gtgtaaccac gacgacgcag
    21181 accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt cggtcaccag
    21241 caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg agaattcgta
    21301 ctggcgcgga tgaacaggaa tggtgatgtt atccagtacc cagtcgtaca gacgacggtt
    21361 gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagcgcat cgctgatgca
    21421 atgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc
    21481 aaatttaatg cggtacagca ccggatcgcg cattacgata aatggcgacg ccatgtcgat
    21541 tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag
    21601 cgccaagttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gctgcgtcag
    21661 ggtaccgcgg tattcgcgga tctgttccgg tgtcagttca tcaacgtacg ccaggccttt
    21721 attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac
    21781 gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat cgacatactc
    21841 gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc
    21901 ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt
    21961 cggctccggc gggaaacggg tgtgtactgt gatgtgctta ccactggcca gatcttcatc
    22021 gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc
    22081 aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt
    22141 agttttttaa aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg
    22201 gcagagaaga tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt cgcatcaggc
    22261 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct
    22321 gagctttaat gagcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    22381 tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    22441 ttacctgcgc accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    22501 cgatacccat atggacgacg atctccgcgc ctttttcggt ttccaggcag aacgcgtggt
    22561 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    22621 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat
    22681 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    22741 gtacagcctg cggttttgct acaggcgcgg cagttgccgg agttgcttca gctgacgcag
    22801 cggctaccgg accacgggca acgactttct tcatcgcatc gccgatggat tctgctttcg
    22861 cgccaacaat cacctgaata gtctgtttgt tcagtttcac tacaccagaa gcacccagac
    22921 gtttacacat cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc agacgggtaa
    22981 tacaggcatc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    23041 ccagttgagt cagaccttct tcagtattgc tgttggcttc ttcagtaacg atctcgtctt
    23101 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    23161 cgaagtagat agcgaagaag ataacgccca tcaccagcag catccagacg ttctggctgg
    23221 cggcaggcag gttatacatc aacgcgtagt cgatagcacc cgcagagaaa gagaagccgg
    23281 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    23341 ggtacagcag cggcgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    23401 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    23461 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    23521 agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt
    23581 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    23641 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    23701 ccagcgcgcc cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtaccggcg
    23761 gccagacgta accaaaaatg gccgccagca ccagacagaa gaagccggtg gcgatcggca
    23821 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    23881 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    23941 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    24001 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    24061 cgaggttatc aaaaattgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    24121 gctgaccgaa tcgcagcaac agcgccgcca ccggcagcac cgcgataggg agctgtaacg
    24181 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    24241 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttttactgcc gatgttctgt
    24301 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc atcgcaaatt
    24361 aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    24421 gctaaaccag aaaacttatt ttatcattca aaaaatcaag tcggattgac gcctgtctgc
    24481 gcaaatccag gttacgctta aagatgtcta atccgccaac ggcttacatt ttacttattg
    24541 aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct
    24601 cgccatatcg tcaatcgtat caatgcgttc aaaccaactg ccgatcgtcc gtttgtactg
    24661 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    24721 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    24781 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    24841 gatattccag cagaaaacat caaccttctc aacggcaatg ccccggatat cgatgccgag
    24901 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    24961 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    25021 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    25081 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacactgct ggatgccgaa
    25141 gaagtgatga ttatggtgct gggtagccag aaagcactgg cgctgcaggc cgccgttgaa
    25201 ggttgcgtga accatatgtg gaccatcagc tgtctgcaac tgcatccgaa agcgatcatg
    25261 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    25321 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggtt ccttgctcag
    25381 ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta
    25441 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    25501 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    25561 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    25621 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    25681 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    25741 tgcgcgttat gcgtgagtac ctggcaaaac atccgaatca ggcgttaggt ctgcatctgg
    25801 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    25861 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    25921 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    25981 tttctgccgg tcactccaac gcgacgttga aagaagcaaa agccggtttc cgcgcgggga
    26041 ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc
    26101 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc
    26161 atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg
    26221 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    26281 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    26341 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    26401 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattga cgttgaaaaa cgtctcggca
    26461 cgctcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca
    26521 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    26581 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    26641 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    26701 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    26761 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    26821 tttccacgca atcggcgtac ggcttggtcg tcatgacgcc accatcactc tgtttgatct
    26881 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcagacgct
    26941 ggaacatgcc ctgctgaatg ccattgctca gtttattgat agctaccagc gcaagctgcg
    27001 cgagctaatc gccatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    27061 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg
    27121 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    27181 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    27241 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat
    27301 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    27361 ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg
    27421 ctaccagagc cgtgtgccac tggacgactg caccatcaaa actatctgca aagccgcgaa
    27481 caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggtaaaac
    27541 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgctg gtgaaatcac
    27601 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aagcgctgaa
    27661 ggcgtttcgt actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    27721 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    27781 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    27841 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    27901 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    27961 aactatcctt cgcagactgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat
    28021 gtgccggata gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag
    28081 gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    28141 ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac acgttcctac
    28201 aactgggaca tgatgcataa agcagcctat ttcgtcgcta acggtgcacg ctttatcgcc
    28261 accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg
    28321 attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc
    28381 cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cagtgattgt cggcgataac
    28441 ctgcgtaccg atattctggc gggcttccag gccggtctgg agacgattct ggtgctttct
    28501 ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg
    28561 tcagtcgctg aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct
    28621 gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa
    28681 tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    28741 acaaatcgca ctttatcagc aatttggccc ccgtaaaata taaccattca ataaaacccg
    28801 ctaaaaatta tcgattcatc aataaataca tcctcacacc agaccctttt tcaccaatcg
    28861 acaatcacca ttacgttttt tatttttgca ctgccaaata gcttgcgcac agtgctgctt
    28921 tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag
    28981 gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag
    29041 aaagcactcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat
    29101 gccagcgata acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg
    29161 gcgcaacctc tctacaacca acaaaaaaac cacgtactgg cggtaaacgg tgaaatctac
    29221 aaccaccagg cattgcgcgc tgaatatggc gatcgttatc agttccagac agggtctgac
    29281 tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag
    29341 ggcatgtttg cctttgccct gtacgacagc gaaaaagatg cctacctgat tggtcgcgac
    29401 catctgggga tcatcccact gtatatgggg tatgacgaac acggtcagct gtatgtggcc
    29461 tcagaaatga aatcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc
    29521 tatttgtgga gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt
    29581 gatgcagtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc
    29641 gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc tactttctgg tggtctggat
    29701 tcctcaatta tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa
    29761 cgctctgaag cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg
    29821 gatcttaaag cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac
    29881 ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat
    29941 gatgtgacca ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg
    30001 atgggcatta aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt
    30061 tacttccata aagcgcccaa cgctaaagaa ctgcatgaag agacggtgcg taaactgctg
    30121 gccttgcata tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa
    30181 gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccgcag
    30241 gataaaatgt gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca
    30301 tacctgcccg caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac
    30361 agttggatcg acaccctgaa agaagtggcg gcgcagcagg tttctgatca gcaactggaa
    30421 actgcccgct tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgc
    30481 gagatctttg aagaactgtt cccacttccg agtgccgctg agtgcgtgcc gggcggtcct
    30541 tccgtcgcgt gttcttcagc taaagcgatc gaatgggatg aagcgttcaa aaaaatggac
    30601 gatccgtctg gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcggtac
    30661 tgccccggag aaatccgggg cagttttagt tattgcgctg cttgttgatt ctgatatgca
    30721 gtaaggaaat gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt
    30781 atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa
    30841 aagaatggct gtagggtggg gaaaaacaca aagtaaacaa taattgacga atatagcgcc
    30901 acgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt
    30961 gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa
    31021 aaagatggct acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg
    31081 ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc
    31141 agatttaggt tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca
    31201 ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg
    31261 gcattccctg gttcgaatcc aggtacccca gccatcttct tcgagtaagc ggttcaccgc
    31321 ccggttattg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg
    31381 ttcgaatcca ggtaccccag ccatcgaaga aataatctgg ctacgtagct cagttggtta
    31441 gagcacatca ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc
    31501 tgaatgtgtc gaatacgttc ggcaaattca aaaaccaatt tgttggggta tcgccaagcg
    31561 gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaat
    31621 ttattcaaga cgcttacatc gtaagtgcac ccagttgggg tatcgccaag cggtaaggca
    31681 ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa
    31741 gctcgcttcg gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgttgac
    31801 acgtcttatc cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggctggc
    31861 acgcatccgg caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc
    31921 aacacgccag cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc
    31981 agattattac tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc
    32041 gccatgcgcc gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag
    32101 ctgcgggcat tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc
    32161 ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc
    32221 tgcacatact gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta
    32281 acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc
    32341 atattctgca actggcgaat acgcgccgga gagtcatacc acaccagcga tgcccagtta
    32401 tcaaacaacg gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg
    32461 ccgggatcgt tctcacactg tacgctaatc aacatgcacg actgcgcata ctgccaggca
    32521 tgaacgccaa ttcccgccat ctgccgcacc tgagaatttg cgccgtcggc accaatcacc
    32581 agtttcgcgc ggatcgtttc accgcccttc agttccagct cctgaagatc attatctcga
    32641 tgtaatgcaa tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc
    32701 tgccacaacg cctgttgcag gactgtgttt tccaccatgt agccaagcaa cggcaacttt
    32761 aattcggctg cgtcaaatac cacatgtgcc gtttcccact cccaggtttc cagtctgcgg
    32821 taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat
    32881 accgaagccg cgctgatcgc cgagatccgc acgtccggtt ggctgtcagc gacaaacggt
    32941 gcaggttctg cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc
    33001 agtgcgccgc cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg
    33061 gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga
    33121 taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg
    33181 ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt
    33241 cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat
    33301 caactgaccg acgtggcgga agaagcggat gtgctactgc tgaacacctg ctcaatccgc
    33361 gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag
    33421 aatccagacc tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt
    33481 cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg
    33541 gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa
    33601 atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc
    33661 atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa
    33721 gaggtaagcc gtccgtctga cgatattctg tttgagattg cccagcttgc ggctcagggc
    33781 gtgcgtgaag tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac
    33841 ggcaccaccg gatcgtttgc cgatctgttg cgtctggttg ctgcgatcga cgggatcgat
    33901 cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac
    33961 cgcgacacgc ctgagctggt gagcttcctg catctgccgg tacagagcgg ttccgatcgc
    34021 attctgaacc tgatgggacg tacccatacg gcgctggagt acaaagcgat catccgtaaa
    34081 ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcattgttgg cttccctggc
    34141 gaaaccaccg aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg
    34201 agctacagct ttatcttctc tgcacgtcca ggaacgcctg ccgccgatat ggttgatgat
    34261 gttccggaag aagagaagaa gcagcgtctg tatattctgc aagaacgtat caatcagcaa
    34321 gcgatggcat ggagccgtcg gatgctcggc accacccagc gtattctggt agaagggact
    34381 tcgcgtaaga gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc
    34441 gagggcacac cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg
    34501 aactctctgc gcggtaaagt ggtgcgaact gaagatgaaa tgggtctgcg catggcagaa
    34561 acaccggagt cggtgattgc ccgcacccgc aaagaaaacg accttggcgt gggttattat
    34621 cagccgtaat tctcaggcct gccgttccgg tgggccttgt tttttctctc tttatcccca
    34681 tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt
    34741 gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg
    34801 aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg
    34861 acaacatcaa gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gataaccact
    34921 ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt
    34981 atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc
    35041 ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca
    35101 aagcggtcaa tatcaaaact aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc
    35161 agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg
    35221 gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc
    35281 gtattctgct gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg
    35341 atttaagcca gaaagtagac ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc
    35401 tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg
    35461 cctatatgcg tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca
    35521 ctaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta
    35581 tcaccggcga cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcatg
    35641 ccattgaagt gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg
    35701 tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg gaagaagcag
    35761 aacaaaaacg taaagcggcg ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat
    35821 gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga
    35881 gagccagttt caaacatggc tgaatgcggt gatcccgcag tttcaggaag agtcggaagt
    35941 gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa
    36001 ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggagatgtc
    36061 gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg
    36121 caaaccactg gaggcgcatt gggcgcatat ggtggtgcac ggcagtctgc atttgttagg
    36181 ttacgatcac attgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat
    36241 gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt
    36301 gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag
    36361 aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag
    36421 aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac
    36481 gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc
    36541 gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc
    36601 cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc
    36661 atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg
    36721 attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg
    36781 gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg
    36841 aaagagtttc gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt
    36901 tccggtctgg tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag
    36961 tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc
    37021 gcactggctt ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa
    37081 gtcgacacta tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa
    37141 actatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt
    37201 caggttcatg tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga
    37261 aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg tattcgcctg
    37321 ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg
    37381 cctgcggcga ttatttcgct gatggggctt caggcgttga cctttaaccg tcgcccactc
    37441 cagtctgccg ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg
    37501 gtctatgtca gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg
    37561 gtgctgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt
    37621 ctgtggccga aaaccacctg gctgcgcgtg gcgattgcca cccctgctct ctggcaagtg
    37681 accgagtttc tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc
    37741 cagattgatg gcccgttaaa agggctggca ccgctaatgg gcgtggaagc cattaacttc
    37801 ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg
    37861 ctggtggtgg ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat ccaatggttt
    37921 accccgcaac cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatcg
    37981 ctgaaatggg acgaaggcca gcttcttaat acgctaaaga tttactacaa cgcaacggca
    38041 ccgctgatgg gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa
    38101 attaatcagc aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg
    38161 ctggtaaccg ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac
    38221 accatcatca cgctgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac
    38281 aaaaaccatc tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta
    38341 gcaccgttct ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg
    38401 ctgtcggcaa atggtattga gcttactgcg gctatttgct acgagatcat tctcgccgag
    38461 caagtgcgcg ataacttccg cccggatacc gactacctgc tgactatctc caacgatgcg
    38521 tggtttggta aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg
    38581 gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg
    38641 cagggtgaga ttcaggcgat gatcccgcag ttcgcccgcg aggtgttaac cactaacgtg
    38701 acgccgacca ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg
    38761 acggcactgt ttggttttgc tgctgtgttg atgagtctgc gtcagcgacg taaataatcc
    38821 cttcttatcg tgccggaata catcccatat ttcggcacgc tcaccggatg cggcatcagt
    38881 gcattatccg gataacaata ttctcctatc caactttggc acatctattg ctttgttata
    38941 caaggcaaag cttgaaccag catcagtaca acccggttgc accacagcaa tccaccggta
    39001 gcaccaaaag agtgcatttt aagaattttg cttcagaatg gtgcggtgca cctcgcaaaa
    39061 acaaacaata aaacatcaat atctttacat ttagccaaac taaatgttac ctaacaggca
    39121 caacactgca caataaagtt gcagacgata acaacacaaa cactcacaac gggtatccat
    39181 gcgttcttaa cgcagaagat aaaggtgttg gatatgcaat tacgtaaacc tgccacagca
    39241 atcctcgccc tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca
    39301 ggcagtacgc tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct
    39361 tcagtacctt tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac
    39421 tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa
    39481 ctgattccga ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt
    39541 gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact
    39601 attttcgtgg tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc
    39661 gacctgaaag gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac
    39721 aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac
    39781 tctttccgca ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg
    39841 gccggtgaac gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag
    39901 tctcaggaag cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg
    39961 gatgacacta tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg
    40021 ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg
    40081 aaagcactgt tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg
    40141 gaaattccag ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt
    40201 ccccatcggg cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac
    40261 gctgctaccc tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt
    40321 tttacaacaa gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca
    40381 ggtgacgatc gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt
    40441 cggcatttta cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga
    40501 actgttccgc aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga
    40561 gctgctgccg gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt
    40621 tttcctttca tccatgctct gcctggggct gtttaccgcc gcccgtgttt gcgaacaggt
    40681 acgcgccgct attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct
    40741 gacgctaccg cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc
    40801 gccgatgact tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg
    40861 tctggtggat atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga
    40921 gtcgtttacc gccatcacgc ttgcgtatgt gttgattaac gcttttatca tgctggtgat
    40981 gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag
    41041 tttgactgga gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc
    41101 ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg
    41161 atgcgcttgt ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc
    41221 cgctcgattc ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg
    41281 caaaatgtgc tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg
    41341 ttttcgatgt ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt
    41401 tcccgtggtc agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa
    41461 ctgattattc tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc
    41521 gtactgttcc aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc
    41581 gcctcaacca ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt
    41641 gtttatttcg ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca
    41701 gcataatgat taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg
    41761 actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg
    41821 gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca
    41881 ccgtcgatgg tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg
    41941 tcgggatggt attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga
    42001 ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga
    42061 aattgctgga gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg
    42121 gcggtcagca gcagcgcgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc
    42181 tgttcgacga accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga
    42241 tggtggaact ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg
    42301 cccgcaaagt ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc
    42361 cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa
    42421 tcctgcatta atcactctgg cgcacatacc caattgcgcg ccatctcacg ctttatcgtt
    42481 tcgcccctcg tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag
    42541 gagcaacaat ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg
    42601 caatagttct cgcactcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg
    42661 gaaaccagac accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc
    42721 gcaccgatat tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg
    42781 cggacaatgt gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg
    42841 caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac
    42901 ctgtcacgat tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc
    42961 cggaactgca tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta
    43021 actggacgcc tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct
    43081 tccagtcagg gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc
    43141 acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg
    43201 aactgctgga tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac
    43261 cgctgcatga cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg
    43321 agcgctgggt tggcgtggaa acacagggga aatataccca gggtatgacg gttgttgatt
    43381 attattatct gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg
    43441 gctttgttga tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta
    43501 agatcaccac gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat
    43561 aaatgcaatg gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt
    43621 aattgccgtc tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt
    43681 aacaccatcc ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt
    43741 atcacggcgt acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag
    43801 ctggcggtta ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc
    43861 attaaaagaa gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt
    43921 tccggcttat ttcagtgatg aacaacgcaa gcatacccgt ttagcagcgg acttagccgg
    43981 gttaaatgcg gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca
    44041 tacccaacaa aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac
    44101 ggtgcttgag tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct
    44161 cggtggcgaa gattttaccc atatgttggt cgatgacgtt ttaaagcgcg cggatgtcac
    44221 aaggaccacg cttaacgaga gtgaactggc agccttgtac gcctgtgtgg aagcggcaaa
    44281 atgtagcaat caatcgccaa tgcacattcg ctggtagtac caggatgaaa cgcgggaatg
    44341 cgaattttac gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt
    44401 gccgattgaa caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt
    44461 gctggttggc ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg
    44521 caaattaccg tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca
    44581 ggccgcctgc cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc
    44641 ttactcgttg ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat
    44701 tgaacgaaac accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga
    44761 acaggattca attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat
    44821 tctggtggaa tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat
    44881 tcgctttagt tatgatatca acgggttgct tgaagttgac gtgcttctgg aagacggcag
    44941 cgttaagtcc agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga
    45001 gagtcggacg cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaatcgcac
    45061 ctttaaagcc aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat
    45121 tggccgggtg atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga
    45181 tgaggtcagg cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc
    45241 ggatgcggcg taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa
    45301 catcgcgatt ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat
    45361 gctggtaatg ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt
    45421 tggctcatcg acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt
    45481 gataatggcc tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag
    45541 catctcttgt ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag
    45601 taacggtgaa tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt
    45661 gcgtttgaag aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg
    45721 taaaatttcc gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg
    45781 ttgaccttgc gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata
    45841 atccgggcaa tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta
    45901 actttcccag tacgcgccga gatcagcaag caggctttcc ccctcgccgc agtacaaacg
    45961 ttgtgcacac tggtaatacc gggcatcttc gctttctggt tcttccgctt gccactgttt
    46021 ggctacagga atgagttcca ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt
    46081 gcgatgtaac tttgcatcat caggaatgaa ccacggtcct tccatcgcca aaaacgcagt
    46141 gacatagtca ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt caacagtctg
    46201 gttctgtaac gcgacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg
    46261 aatggcctca agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga
    46321 atgccagttc aaacgctctg ccaacaaaga agcacaattt aatgacagat atcgcgcttc
    46381 tatagcgatt gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt
    46441 taattgctga ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag
    46501 taatgtcctg aatgcacgta gtatttcatg ttcagcggtg gcatcttttt cttcatcatc
    46561 tgcttcctcg acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc
    46621 tttaaacccc tgcggatcgg tttcgggatg acacaacggt aagcgagcaa gataagcctg
    46681 gcggataatg tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat
    46741 aggtcgctca catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag
    46801 ggaagcgtgg catagaaacg gtcgcttccc gctgccagct cttcgaacat ccgactcccg
    46861 gcgctatcgt atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga
    46921 gaagattgcc agtagagacg ccctagtgaa taggcagaaa aatagtgcat ccagctgtga
    46981 tagtaatggt gagctcgtgc atagacgcgc gattgcagcc ataaactctc ttcttccgtc
    47041 agccagttgt tgagcacacc ggcccgtgac agaaacccca ttcgaacata atcccaggct
    47101 ttgattcccc cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac
    47161 cctctctccg agccaccagc aattaatgct ttccactctt caggggaaga gcgttgccat
    47221 gtcagatata agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc
    47281 tgaattaaat catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc
    47341 ccggtataca tttttggcga gtcatgcgcg ggtttgcaac tctgattaag tatttccatg
    47401 ggcgcagaaa gcgcaaaaag aagatattgt tcttccttat tcatttttta ataatccctt
    47461 aagtgcatta actgactact tttttattat tgatgagtat aaacttgacg ctttcaaaat
    47521 aaaaagaaaa tcgaagcatt cacacatgaa taaaaaatta atgtatatat tcgcaatttt
    47581 tatagttgca gcaattacct gtattagcca acccaagaaa acgatgttgc gtgataaagc
    47641 catggtgaat tatgcctttg attatttaag atcaccaggc agtctaccgt taaccacggc
    47701 agccacggag ctttccgcga ttcatggtca ctcaacatcg caatatcgcc ttggcgaatt
    47761 ttatcttcat ggtagcgacg gtaaaccact ggattataca caggcgagat actggtatga
    47821 gcaatcagca gaacaggaaa atccgcgcgc acaaagcaaa ctgaggtgga tctacctgaa
    47881 aggcctggga gtcaaacccg atacccgtaa agcaattctc tggtataagg aagcagctga
    47941 acaagggtat gctcatgctc aatatacttt gggtttgatc tacagaaatg gcactggtat
    48001 taatgttaac cattatgaat ctcaaaaatg gttaaaactg gccgccaaac aacattacaa
    48061 aaatgcggaa agattacttg ccggacttcc cgcacattaa cgtcgactgt cacccgaaga
    48121 gatggagtat tttccctatc aaaccggcaa agataaaaat gtagaaaata attttgatga
    48181 cgcccagaca gcccagcccg ccatcatcaa cgggcattgg ctgtggttgc ggctccggct
    48241 cgacggtttc ctgaccggta aaattcgccg cctgaattaa taatcgacga ataattaatg
    48301 cttccagtgg acaacgatct tctgcatcaa ggcggctcac aatcggcatt aatgattctt
    48361 ctgtcggttg gctggcctgc actaattgtt tggcaactaa atataatggc cataagggat
    48421 gttctgtatc acgcgtgagt gagatccgcg gcttacgtcc ttcgtgcaaa accatctcga
    48481 ccagtgaacg ccccagcgca gagagttttt catcgctgag tatttcgtat aaatgaaaac
    48541 gcgtacgggc gctgtcgccg gggacataaa gtagcggttt tccctgatcg ttaaccagat
    48601 cacgcgcttc aatcatcatc accactaatg ggtaataata gacccgatgc tgacggcacc
    48661 attgcaacat taaagcatcc gcctgggtgg agggatactc gcgccagtag taacaaagtt
    48721 ctgccagcag gctttcgcct tctccacaat agacgcgttg tgcgtattca taataacgcg
    48781 catcttcatt gtcaggttca gcttcccgcc acgaccgggc gtaatccagg agttcgggga
    48841 ttcccaggcg gcttgcgcta taccagcgga gtaaatttct gtgcagtttt gcatcatcgg
    48901 ggataatcac tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga tgcgatgacc
    48961 aaatccgagc gaacatttgg taaaaatcga tggtgtcatt ttgcacggca actggcagat
    49021 gcagaatggt ctggaagtta aaaacattcc ccttcgcaat ggcatataaa aaggattcca
    49081 gttcttcttc gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat ctttctgcta
    49141 ataacaccac gcactcgaag gataaatggg cagtgttcat ggcgattgtg cacagcgacc
    49201 agcgtaattc atcaatctct tccatcgagc aataatttaa ttgctgaatg aatcgctgcc
    49261 aggcggagga cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag gcgagcagaa
    49321 tttcatgttc tgctacctca tattcttctg gttgccaaac agatttagca ggcgactgcg
    49381 caatccgtag cgcctcctca tacgcctgac gaagttgttt aaaaccctgc ggatcggttt
    49441 ctggatgaaa ggacggtaac agcgccagat aagcgcggcg gataatatcg acgtcagtcg
    49501 tttcctctat atcgaggatc ttccagcaat ttttcataag tcgctcatat ccttaagcgt
    49561 ttccgggcat gtggggtagt cagccagtgg ttgccaggac aaggtagggt aatacgcatc
    49621 atcactggca aataattgat aaaacatatc attgccggaa tcgtcgtatt ctttacgcgc
    49681 taagaattcg cgtaataaag gcagatgatc atcttcggga gactgccaat attgccgacc
    49741 aaaggtataa ccggcaaagt attgccgcca gttactataa taacgtagag cccgtagatg
    49801 aatacgcgac tggatccata gactttcttc ttcactcaac cagttattaa gtacgcccat
    49861 tcgacttaaa aaacccatcc ggacataatc ccatgcttta attcctccct caccacagca
    49921 ttcggctgtg ctggcgacaa attgcgcata agcctgacct tgctcattta attcagcaag
    49981 atagtcacgc cattcacatg gagaataacg aaaccagcgg tgataaaacc ctgccaaccg
    50041 agcggcatga ccgttctcga tcattcggtg aattatgcgc agcaggtcat cacggtcatc
    50101 aattccccat gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt tgggggatga
    50161 atgcgaaggt aaacagcatt cattgcgtat ttccatcgga gtggaaagtg caaacaatat
    50221 tttttgcgac cccatgtcca tataatttat ccttaaacat tcatttaatg aatgtaaact
    50281 acatttcgag gctcagttat ttatactttt ctgacgcaac ataatacgca taaaataagg
    50341 cttacgcaaa aatggaatac ataaaaatat aatatatttt atctacaacc ataaggaaat
    50401 gataatgatt tttacgtcaa gttgctgcga taatttatca atagatgaga ttatcgaacg
    50461 tgctgaaaaa ggcgattgcg aggctcaata tattgttggg ttttattata atcgcgatag
    50521 cgcaattgac tctccggacg acgaaaaggc cttttactgg ctgaagctgg ccgctgagca
    50581 aggtcattgt gaagcacagt attccttagg gcaaaagtat accgaggata aaagccgtca
    50641 taaagataat gagcaagcca tcttctggct gaaaaaagct gccctacaag gccatacttt
    50701 cgcttccaac gcccttggct ggatactgga tcgtggagaa gaccccaact ataaagaagc
    50761 ggttacctgg tatcagatag ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg
    50821 atggatgtac agaaatggca acggagtcgc acaagactat acgctggcat ttttctggta
    50881 caaacaagct gcattacaag gccatagtta cgcacaagac aatctggccg atctttataa
    50941 agacggagaa ggcgttgctc aaaacaagac actcgccgca ttctggtatt tgaaaagcgc
    51001 acagcagggt aatcggcacg cccagtttca aattgcatgg gattataacg ctggcgaagg
    51061 ggtggaccag gactataagc aagcgatgta ctggtatctg aaggctgccg ctcaggaaag
    51121 cgtcggcgct tacgtcaaca tcggttatat gtataaacac ggacaaggcg ttgagaaaga
    51181 ttatcaggct gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg
    51241 gtataacctg gccattatgt atcactacgg agaaggaaga cctgtcgatc tccgacaggc
    51301 tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg
    51361 tgagactgaa gctttactgt agaaataaaa acaagggccg gggaatttca ttctgcccat
    51421 attctcttta ttgctgtgcg aaatcagact attacggctc aaacgggcgt ctctggaact
    51481 ggtcataacc acatttcggg catagcgtca gcacttccgg tgtgtagatc gggagatgga
    51541 agtgacattt ctcgcagacc agatttccca gcccgaccac ttctccgctg tgataaaccc
    51601 catgatgatt gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta tcggtgatgt
    51661 ctgccagctc ctgccacaag ctttctttaa tcacccgcat aaagacgctg tcagattctt
    51721 ctttcaggct ctcttcatag ctcatggcga actcttccaa gtcacgtctg acagctcgcg
    51781 tcagctcatc gatctcggtt cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct
    51841 gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc agtgacgcga
    51901 ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg tcgttaactg
    51961 actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa actctgtaaa
    52021 ttaatgtacg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac
    52081 gcggacgttt gggctatgct atgcggatct gaaaaaccac ctcaacgcta catttgtagc
    52141 cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga
    52201 atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag
    52261 caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg
    52321 ccacgtacgt aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa
    52381 aaacgttctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt
    52441 gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca
    52501 gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga
    52561 atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa
    52621 aaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt
    52681 tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg
    52741 gtttatcaaa atcactgctt acgctgacga gctgctcaac gatctggata aactggatca
    52801 ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga
    52861 gatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga
    52921 cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc
    52981 ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc
    53041 cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta aagctgttca
    53101 cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg
    53161 cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt ttgcctctaa
    53221 atacggcctg aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc
    53281 tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga
    53341 ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg
    53401 taaagtgaac taccgcctgc gcgactgggg tgtttctcgc cagcgttact ggggcgcgcc
    53461 gattccgatg gtgacgctgg aagacggtac cgtaatgcca accccggacg accagctgcc
    53521 ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc
    53581 ggagtgggca aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga
    53641 cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg
    53701 tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat
    53761 cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc
    53821 aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc
    53881 agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc
    53941 tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga
    54001 actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca
    54061 ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc
    54121 ggctgatatg actctcgaat ggcaggaatc cggtgtggaa ggggctaacc gcttcctgaa
    54181 acgtgtctgg aaactggtgt acgagcacac agcaaaaggt gatgttgcgg cactgaacgt
    54241 tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa
    54301 agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga
    54361 gctgatgaac aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca
    54421 ggaagcactg caggccgttg tccgtatgct caacccgttc accccgcaca tctgcttcac
    54481 gctgtggcag gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga
    54541 cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg
    54601 tgccaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca
    54661 ggaacatctg gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc
    54721 aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca
    54781 acactgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac
    54841 accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg
    54901 ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa
    54961 gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat cgcgaaagat
    55021 accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat gacggtgaat
    55081 gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta gcgccaaagt cttccgttca
    55141 ttcttcgata acccgcaaat ggcgttagcg aaagataacg aacaagacat gatcgtaaaa
    55201 gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg
    55261 gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc aacgcctgca
    55321 cgcgtctcca ccatgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc
    55381 agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc
    55441 aggaaagcca ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa gaacaccaca
    55501 ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc caggctatga
    55561 gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaatgcgg
    55621 cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg ctattgatcg
    55681 tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga
    55741 atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg
    55801 ctgtgcgcgc aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct
    55861 actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct
    55921 ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt
    55981 tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata
    56041 ttcttcagca actgcgtctg gaaggcagcg agccggttat tttgttgcgc acattacaac
    56101 gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt
    56161 tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc
    56221 gcttaagtca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc
    56281 tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct cttctgttgt
    56341 gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg
    56401 tttggcggca cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg
    56461 aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc gcatcgtccc
    56521 cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat tgccgacaag
    56581 ccattattta ctcttgatga acgcgagcta aagcgcaatg cctcctctta cactgcgcaa
    56641 acactgaaag agtggcggca ggaacaagga ccggacgtgc cgctggcgtt tattattggt
    56701 caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca
    56761 catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag
    56821 caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt
    56881 aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt
    56941 ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac cggtattgac ttacattaac
    57001 caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa
    57061 ttcgtcatgg cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac
    57121 ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt
    57181 cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc
    57241 tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg
    57301 actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt
    57361 ggtgcaatga ctggcagcat gctatcccca cgaatggtga aggatttcag gcattttcgc
    57421 aacgtgtgga acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa
    57481 tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tttaattggc atgcctgccg
    57541 agtctatgtg gcattttcgc gttgaccagg ggtgttggag cgccattgat atcaaccaaa
    57601 aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt
    57661 tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct
    57721 ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg
    57781 atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa
    57841 ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc
    57901 acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat
    57961 gacctcaaag gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac
    58021 tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt
    58081 gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc
    58141 gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt
    58201 cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg
    58261 ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg
    58321 aaagatatgc ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac
    58381 atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc
    58441 attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg
    58501 gaacgctgga agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg
    58561 tcgcctgcct gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc
    58621 catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc
    58681 aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa
    58741 ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg
    58801 gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg
    58861 caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg
    58921 cctatcgcgc ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac
    58981 cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg
    59041 gacgctttgc gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag
    59101 cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa
    59161 gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa
    59221 cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc
    59281 aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg
    59341 catgatattg gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc
    59401 ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta
    59461 ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt
    59521 gaaacgctgc tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg
    59581 gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc
    59641 agcaaagatt attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca
    59701 cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc
    59761 gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg
    59821 tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga
    59881 tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat
    59941 cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg
    60001 gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg
    60061 tggtatcagg gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca
    60121 atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat
    60181 ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg
    60241 cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt
    60301 gctaaccgcc ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg
    60361 gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac
    60421 aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat
    60481 ccgcaagtgg ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg
    60541 ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg
    60601 gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa
    60661 aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc
    60721 tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc
    60781 gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc
    60841 cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg
    60901 cggtagatgc tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg
    60961 ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta
    61021 tcaaccgcga cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat
    61081 ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc
    61141 tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag
    61201 tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc
    61261 gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca
    61321 ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg
    61381 gcactcagtc acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac
    61441 tggcggaaga gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga
    61501 tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg
    61561 gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta
    61621 ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg
    61681 tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga
    61741 aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga
    61801 atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct
    61861 gcggtatgta acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg
    61921 aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat
    61981 ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac
    62041 ctgacggcct ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt
    62101 ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc
    62161 attaatgatc gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct
    62221 gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag
    62281 gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc
    62341 ctgcctgcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag
    62401 ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg
    62461 gtaaccagta gcggtttcct cggcgctcca acgaccttag cgcctagtgt actggaaggc
    62521 agcgaaccga cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc
    62581 tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg
    62641 caagtcgggg ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag
    62701 aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc
    62761 ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa
    62821 ttacagtcat ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc
    62881 tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct
    62941 atagtagggc acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt
    63001 ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc
    63061 tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc
    63121 gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac
    63181 gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc
    63241 caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca
    63301 tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg
    63361 caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt
    63421 gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc
    63481 tacgttaacg cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct
    63541 gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac
    63601 gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag
    63661 ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga
    63721 cacactgaca aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg
    63781 atttctgcgg tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg
    63841 ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc
    63901 gcctctgaac cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac
    63961 gtgtacctga ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac
    64021 agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag
    64081 cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt
    64141 aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa
    64201 aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact
    64261 cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca
    64321 aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt
    64381 tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca
    64441 ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg
    64501 ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc
    64561 gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc
    64621 tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat
    64681 caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccgggct
    64741 atgcatgaat tcaccgatac ccgcgataat agtacccttg atgaaatctg gctggtcgag
    64801 cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt
    64861 gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa
    64921 caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg
    64981 accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct
    65041 cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga
    65101 attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca
    65161 tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg
    65221 aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta
    65281 ctaaacaatc cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt
    65341 acactgggtc attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat
    65401 tttctttatc acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat
    65461 taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat tagacataag
    65521 attattgtaa ttttttaaat attattttcc catgaataaa attggagtaa gtgcgtggat
    65581 agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata
    65641 tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta
    65701 cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag
    65761 tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt
    65821 gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc
    65881 attcttggcg cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg
    65941 accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat taaaactcac
    66001 tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag
    66061 tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat
    66121 gttctgttca ccgacaatat ggtgttaatt tgccgcaagg gaaatccact actctcttta
    66181 gaagatgaca gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagggcaa
    66241 aatttcagcg gtctgcggca gagagttcaa gagatgtttc cggaccggca aatcaatttc
    66301 accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt
    66361 attcccagcc gtttttataa cctgtttagt cgttgctggc cgctggaaaa actgcctttt
    66421 ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt catcctgcgt
    66481 gatcccatcc tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt
    66541 acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt
    66601 cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca
    66661 aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa
    66721 ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga
    66781 tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct
    66841 gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat
    66901 caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa
    66961 cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac
    67021 ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga
    67081 accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc
    67141 cgttgaccgt gatgacctgc gcgatggcgg tgcccaacac tttgcggatt gtattactgc
    67201 cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg
    67261 aatggatcgt gctctggata ttctgactgc aacgccccca gatgtgttca accataacct
    67321 ggaaaacgta ccgcgtattt accggcaggt acggcctggc gcagattaca actggtcgct
    67381 gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat
    67441 ggtgggtctg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca
    67501 tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt
    67561 tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg
    67621 ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc
    67681 gaaaggaatg gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc
    67741 cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca
    67801 cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc
    67861 gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg
    67921 cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc
    67981 agtttggtaa tactaatctc acccatagat accttcttga catataaccg gcaacagaac
    68041 gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg
    68101 taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc
    68161 gacggttgtt taagatgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt
    68221 ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta
    68281 taatccggct ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg
    68341 tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac
    68401 gaacccaggc ggcaggaagt accaggattt cagctccctg taaagcctgt gccagcgcca
    68461 gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca
    68521 gtaacggagc tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat
    68581 catagagatg cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca
    68641 tattccatgc gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc gtcatcatgt
    68701 tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt
    68761 taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca
    68821 gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt
    68881 cccacacaga tgtaacagca aactgtcctg cagcaaccag cattgccacc tcctgtcaga
    68941 gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa
    69001 tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga
    69061 agcgtagcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg
    69121 gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc
    69181 agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc
    69241 ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc
    69301 tttggctggg cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca
    69361 ctggcattct ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa aacccgctga
    69421 ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa
    69481 gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta
    69541 aaaccattag tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga
    69601 gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac
    69661 atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttat cagtacacca
    69721 attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt
    69781 tgatgttgct tattttagtt attgatttta aagaagttac taaaacttca tttgtctcaa
    69841 aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag
    69901 gtcatctgaa aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga
    69961 gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga
    70021 aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc
    70081 catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca
    70141 tcccaacgcg acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt
    70201 tctttgtcgt aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt
    70261 tcaggctgtt gccaggtttg tgcaatattt tctctaaacg ttgtcatcct ctcatctgcg
    70321 ttagcaaaaa ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca
    70381 tatttactca cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag
    70441 caaagctaca attaacataa ccttaataga cccaacataa agaataatct gaatagctgc
    70501 tctatttatt tccgcttgtt caagttaaat atttccgcgc atcgattaaa gatgatgcgt
    70561 cgttttctgc gatgggaata gtcaaaaaag aaaaaccaag tcttttttga tgacaaaatg
    70621 caatcaagga aaaattattt tattttttta acgagataca acaatcatct taacgaagta
    70681 tataatatat atcgtcaacc atgctcatat tagattattt tttgagcgca cagccgcagc
    70741 taacaccatt gcaattaaca aatttgcatc aatccaccat caatttacac acattattat
    70801 gtgataattg ccaaccgcta aatatgcgtt ttgttatcta tgtataaaaa cagcaacttc
    70861 aatgtcttaa tggcagtttt tcttgatttt aatcagcatt catcgccaat ttattgggca
    70921 tattttttcc ttaagcttta ggaagttttt attatttact ttgggcctgg agacaggaaa
    70981 aattatgctg acattcattg aactccttat tggggttgtg gttattgtgg gtgtggctcg
    71041 ctacatcatt aaagggtatt ccgccactgg tgtgttattt gtcggtggcc tgttattgct
    71101 gattatcagt gccattatgg ggcacaaagt gttaccgtcc agccaggctt caacaggcta
    71161 cagcgccacg gatatcgttg aatacgttaa aatattacta atgagccgcg gcggcgacct
    71221 cggcatgatg attatgatgc tgtgtggctt tgccgcttac atgacccata tcggcgcgaa
    71281 tgatatggtg gtcaagctgg cgtcaaaacc attgcagtat attaactccc cttacctgct
    71341 gatgattgcc gcctattttg ttgcttgtct gatgtcactg gccgtctctt ccgcaaccgg
    71401 tctgggtgtt ttgctgatgg caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg
    71461 tggcgcagca gctgccattt gtgcctcccc ggcggcgatt attctcgcac cgacttcagg
    71521 ggatgtggtg ctggcggcgc aggcttccga aatgtcgctg attgacttcg ccttcaaaac
    71581 gacgctgcct atctcaattg ctgcaattat cggcatggcg atcgcccact tcttctggca
    71641 acgttatctg gataaaaaag agcacatctc tcatgaaatg ttagatgtca gtgaaatcac
    71701 caccactgcc cccgcgtttt atgccatttt gccgttcacg ccgatcatcg gtgtactgat
    71761 ttttgacggt aaatggggtc cgcaattaca catcatcact attctggtga tttgtatgct
    71821 gattgcctcc attctggagt tcctccgcag ctttaatacc cagaaagttt tctctggtct
    71881 ggaagtggct tatcgcggga tggcagatgc gtttgctaac gtggtgatgc tgctggttgc
    71941 cgctggggta ttcgctcagg ggcttagcac catcggcttt attcaaagtc tgatttctat
    72001 cgctacctcg tttggttcgg cgagtatcat cctgatgctg gtattggtga ttctgaccat
    72061 gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat
    72121 cccgaaactg gcgcactctt ccggcattaa cccggcgtat ttgactatcc cgatgctgca
    72181 ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc gtagtcgttg cggttgccgg
    72241 gatggcgaaa atctcaccat ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg
    72301 gctagtgatt gttatcgttg ctacagagct gatggtgcca ggaacggcag cagcggtcac
    72361 agacaagtaa atagtaatgc cggaggcaag tttcctccgg ctttatcatg tcacccactg
    72421 tggtatatgc gttgtggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg
    72481 cggctggcgc aatattcaag ataacgcctg gcagtggtgc ggctgatggt cagtgcctgc
    72541 gccaccgttt ctgccgtatg ttgcacacca ggctctttaa acagttttcg caccgcgttt
    72601 agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg gttcaccgcg agcataggca
    72661 ttaaacatct catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta
    72721 cgctggcgga aacgggttag cgtttgcccc agtgtattga ttcacttgaa gtacgaaaaa
    72781 accgggagaa cattggatta ttcgggatct gatgggatta gatttggtgg ggcttgcaag
    72841 cctgtagtac aaattttagg cgttaaccaa tgaaacgcga aagatagtaa aaaattgctt
    72901 ttgtttcatt gaaaaaacga aaaacaaaaa cactataaat catttcaatc atagcttcaa
    72961 aaaacgttca aaaccgataa caaccaggct gtcaccaaat gactcatatc acaaatcagc
    73021 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggc
    73081 tcatggttgt tatacggctt ttttcgcatc ttgtggttcc aacaaaggag ctacaggctc
    73141 ttcctgttta tgaacaccat acattgacaa aatctcttgg cgagtttctg gaggtaattt
    73201 atgcagcgcc aaagctgttt gtagtgtttg gcgatcaata cccagcgttg ccaatagggt
    73261 gtcaacggag atggaggggc gttcttgcat taatccggaa agcccatatt tagccatgtt
    73321 tagctctatc tctcggactt cattttctcc tagtttattg attaacaggg agaacatttt
    73381 caatgtatca gcattcattt cttcgatagc aattttttgc atagccggta ttgccgggat
    73441 atattcaacc ccaggcccgc gacgcccttc ttgtgaacgc ttcggccatc cttctttctt
    73501 tgctcgttta tgcaccccct gtattgtccc aggcattcca tccagttcag taatttgttt
    73561 gggtgtcatc cactggttta tctcatttgc gcttttcata aactaagttt accagattta
    73621 tctatataaa aatcaatgga ttacaaaata aaccacatta actgatataa accggttttg
    73681 tttatttggt aatgtataaa acagataatc aagacgaatt atttgttttg gtaacccttt
    73741 atcatacgca atatggttgg tgtgcgaaag atgagtaacg attggcatcg ggccgacgtg
    73801 attgcggccc tcaaaaaaac aggaacccct ctggcaaaat tgtccagacg ttcaggttat
    73861 tcaaccggga cgcttgcaaa tgcattagtc aggccatggc ctaaaggtga acgcattatt
    73921 gctgacgcct taaacatgaa acctgaggaa atttggccta gtagatatct aagggatcac
    73981 aatgagagag tggatatcag caattgaatg catgggttta tctggtttac ctggttcatt
    74041 aatgggggtt cacaaaagag ctcagagaga aggatggctc aagagaagaa gagaaggaat
    74101 tagaggggtt gcttacgaat accatatatc ttctcttcct aacattgctc gagctgaagt
    74161 tttgctccaa caaggaaaat ttaaaaccag tcagggatgt tttgaaatcg cccgccccac
    74221 gctggaagct catgattatg atcgtgaggc actatggagc aaatgggata aagccagcga
    74281 ttcccagcgc agcctggctg aaaaatggtt gccctcaatt caggccacag acgaaatgct
    74341 gaaccagggg atttcaacga aaacggcttt tgcgaccgtt gcagggcatt accaggtcag
    74401 cgcatccact ttgcgggaca agtattacca ggtacagaag tttgcgaagc ctgactgggc
    74461 ggctgcactt gttgatggac gtggagcatc ccgtcgcaat gttcacaaaa gtgaatttga
    74521 cgaggatgcc tggcagtttc tgattgcaga ttatctgcga ccggaaaaac ccgccttccg
    74581 caaatgttat gagcgtctgg aactggcagc ccgcgagcat ggctggagta ttccctcccg
    74641 tgccacggcc tttcgccgga ttcagcaact ggacgaggca atggttgttg cctgtcgtga
    74701 aggtgaacat gcactgatgc atctgatacc ggcacagcag cgaactgtgg aacacctgga
    74761 cgccatgcag tggatcaacg gcgacggtta tctgcataac gtctttgtac gctggtttaa
    74821 cggtgatgtg atccgtccga aaacatggtt ctggcaggat gtgaaaaccc gaaaaattct
    74881 gggctggcgc tgcgatgtga gcgagaacat tgattcaatt cgcctctcgt tcatggatgt
    74941 tgtgactcgc tacagtatcc cggaggattt tcacatcacc attgataaca cccgtggtgc
    75001 tgcgaataaa tggctgacgg gaggcgcgcc caatcgctac cgctttaagg taaaagagga
    75061 cgatccaaaa ggactgtttt tactgatggg tgcgaaaatg cactggacaa gcgttgttgc
    75121 cggtaaaggc tggggccagg caaaacctgt tgaacgtgct ttcggtgttg gtgggcttga
    75181 ggaatacgtt gataagcatc cggcactggc tggcgcatat acggggccaa atccgcaggc
    75241 aaaacctgat aactatggcg accacgctgt tgatgcagag ctgtttctga aaacctttgc
    75301 cgaaggtgtg gcgatgttca atgccagaac aggccgtgaa acagaaatgt gcgggggcaa
    75361 actctcgttt gatgatgttt tcgagcgtga atacgccaga acgattgtgc gtaagccaac
    75421 cgaagaacaa aaacggatgc tgttactgcc tgccgaggcg gtgaacgttt cacgcaaagg
    75481 cgagtttacg cttaaagttg gcggctccct taaaggcgcg aaaaacgttt attacaacat
    75541 ggcattaatg aatgccggag tgaaaaaagt tgtggtcaga tttgatccac agcagttaca
    75601 cagcacggtt tattgag
//
LOCUS       DAJTNV010000003        72935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNV010000003 DAJTNV010000000
VERSION     DAJTNV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO199_000160"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO199_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5534349.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO199_000161"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO199_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5534350.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO199_000162"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO199_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5534351.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO199_000163"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO199_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5534352.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO199_000164"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO199_000164"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5534353.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO199_000165"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO199_000165"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5534354.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO199_000166"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO199_000166"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5534355.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO199_000167"
     CDS             complement(4598..5320)
                     /locus_tag="OO199_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5534356.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO199_000168"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO199_000168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5534357.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO199_000169"
     CDS             6520..6738
                     /locus_tag="OO199_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5534358.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO199_000170"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO199_000170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5534359.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO199_000171"
     CDS             complement(7472..7672)
                     /locus_tag="OO199_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5534360.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO199_000172"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO199_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO199_000173"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO199_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5534361.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO199_000174"
     CDS             10101..12014
                     /locus_tag="OO199_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5534362.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO199_000175"
     CDS             12014..13036
                     /locus_tag="OO199_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5534363.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO199_000176"
     CDS             13030..13248
                     /locus_tag="OO199_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5534364.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO199_000177"
     CDS             13302..14171
                     /locus_tag="OO199_000177"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5534365.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO199_000178"
     CDS             complement(14226..14630)
                     /locus_tag="OO199_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5534366.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO199_000179"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO199_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5534367.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO199_000180"
     CDS             15615..17705
                     /locus_tag="OO199_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5534368.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO199_000181"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO199_000181"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5534369.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO199_000182"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO199_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5534370.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO199_000183"
     CDS             complement(19673..20275)
                     /locus_tag="OO199_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5534371.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO199_000184"
     CDS             complement(20265..20432)
                     /locus_tag="OO199_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5534372.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO199_000185"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO199_000185"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5534373.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO199_000186"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO199_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5534374.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO199_000187"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO199_000187"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5534375.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO199_000188"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO199_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5534376.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO199_000189"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO199_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5534377.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO199_000190"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO199_000190"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5534378.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO199_000191"
     CDS             28269..28436
                     /locus_tag="OO199_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5534379.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO199_000192"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO199_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO199_000193"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO199_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5534380.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO199_000194"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO199_000194"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5534381.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO199_000195"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO199_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5534382.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO199_000196"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO199_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5534383.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO199_000197"
     CDS             complement(33764..34849)
                     /locus_tag="OO199_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5534384.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO199_000198"
     CDS             complement(34861..36165)
                     /locus_tag="OO199_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5534385.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO199_000199"
     CDS             complement(36177..36530)
                     /locus_tag="OO199_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5534386.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO199_000200"
     CDS             complement(36541..37419)
                     /locus_tag="OO199_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5534387.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO199_000201"
     CDS             complement(37416..38642)
                     /locus_tag="OO199_000201"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5534388.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO199_000202"
     CDS             complement(38643..39806)
                     /locus_tag="OO199_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5534389.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO199_000203"
     CDS             complement(39890..40252)
                     /locus_tag="OO199_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5534390.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO199_000204"
     CDS             complement(40269..41174)
                     /locus_tag="OO199_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534391.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO199_000205"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO199_000205"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5534392.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO199_000206"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO199_000206"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5534393.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO199_000207"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO199_000207"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5534394.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO199_000208"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO199_000208"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5534395.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OO199_000209"
     CDS             complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OO199_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5534396.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OO199_000210"
     CDS             complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OO199_000210"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5534397.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OO199_000211"
     CDS             complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OO199_000211"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5534398.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OO199_000212"
     CDS             complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OO199_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5534399.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OO199_000213"
     CDS             complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OO199_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5534400.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OO199_000214"
     CDS             complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OO199_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5534401.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OO199_000215"
     CDS             complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OO199_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5534402.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OO199_000216"
     CDS             complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OO199_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5534403.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51681..54233
                     /gene="mrcA"
                     /locus_tag="OO199_000217"
     CDS             51681..54233
                     /gene="mrcA"
                     /locus_tag="OO199_000217"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5534404.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OO199_000218"
     CDS             complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OO199_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5534405.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55278..57413
                     /gene="igaA"
                     /locus_tag="OO199_000219"
     CDS             55278..57413
                     /gene="igaA"
                     /locus_tag="OO199_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5534406.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57478..58146
                     /gene="yrfG"
                     /locus_tag="OO199_000220"
     CDS             57478..58146
                     /gene="yrfG"
                     /locus_tag="OO199_000220"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5534407.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58157..58558
                     /gene="hslR"
                     /locus_tag="OO199_000221"
     CDS             58157..58558
                     /gene="hslR"
                     /locus_tag="OO199_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5534408.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58583..59452
                     /gene="hslO"
                     /locus_tag="OO199_000222"
     CDS             58583..59452
                     /gene="hslO"
                     /locus_tag="OO199_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5534409.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59596..61320)
                     /locus_tag="OO199_000223"
     CDS             complement(59596..61320)
                     /locus_tag="OO199_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5534410.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61699..63321
                     /gene="pckA"
                     /locus_tag="OO199_000224"
     CDS             61699..63321
                     /gene="pckA"
                     /locus_tag="OO199_000224"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5534411.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OO199_000225"
     CDS             complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OO199_000225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5534412.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OO199_000226"
     CDS             complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OO199_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5534413.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65693..66169
                     /gene="greB"
                     /locus_tag="OO199_000227"
     CDS             65693..66169
                     /gene="greB"
                     /locus_tag="OO199_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5534414.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66266..68587
                     /locus_tag="OO199_000228"
     CDS             66266..68587
                     /locus_tag="OO199_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5534415.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69025..69252
                     /gene="feoA"
                     /locus_tag="OO199_000229"
     CDS             69025..69252
                     /gene="feoA"
                     /locus_tag="OO199_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5534416.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69269..71590
                     /gene="feoB"
                     /locus_tag="OO199_000230"
     CDS             69269..71590
                     /gene="feoB"
                     /locus_tag="OO199_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5534417.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71590..71826
                     /gene="feoC"
                     /locus_tag="OO199_000231"
     CDS             71590..71826
                     /gene="feoC"
                     /locus_tag="OO199_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5534418.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72029..72916
                     /gene="rpnA"
                     /locus_tag="OO199_000232"
     CDS             72029..72916
                     /gene="rpnA"
                     /locus_tag="OO199_000232"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5534419.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17576 a        19764 c        18451 g        17144 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgcccccc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt
    45301 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt
    45361 ggacggacgt tcagcggttt gcgttttcgc cgagtcacgc gatgcattgc cattgcgaac
    45421 aggcgcaacc gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg
    45481 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    45541 ttgaccatcc gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag
    45601 cgaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    45661 cgcctgatcg gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc
    45721 gctggaagtg gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    45781 aaccagaatg ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    45841 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    45901 gtcaatttct tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg
    45961 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    46021 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    46081 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    46141 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    46201 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    46261 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    46321 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    46381 cattgaccgg taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac
    46441 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    46501 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    46561 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct
    46621 tcagttcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    46681 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    46741 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    46801 ccaccaccac tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    46861 tgaccgcagt tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt
    46921 gaatgaaacg aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta
    46981 cgccgccgcc aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg
    47041 taaagacggt atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc
    47101 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    47161 ccagggtttc gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    47221 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    47281 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    47341 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    47401 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    47461 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    47521 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    47581 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    47641 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    47701 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    47761 ctcttgatcg gaatcgtaaa attccatatt gagctgttga gctaactggc gcccaatagt
    47821 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    47881 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    47941 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    48001 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    48061 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    48121 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    48181 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    48241 ttcagcataa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    48301 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    48361 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    48421 taaaaatgcc gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct
    48481 cctgcttatc aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt
    48541 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct
    48601 ccatccccag gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa
    48661 cctgatatgg aatttcgctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca
    48721 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    48781 ccagcaagcg tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta
    48841 cggagaggtc gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg
    48901 ccagcgtcca tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg
    48961 ccgacagctc aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc
    49021 ttaacgccgt tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac
    49081 tccctttggc actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt
    49141 gcagggttat actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct
    49201 cctggcgggc gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt
    49261 cctgccgcgt tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    49321 catctgttag atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact
    49381 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    49441 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    49501 tccattgctt catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt
    49561 gtcccggtac ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc
    49621 agcacatcgt tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg
    49681 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    49741 catagatctt ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata
    49801 cctgccaaca accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag
    49861 atcatcacct tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg
    49921 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc
    49981 ggatggatgc caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    50041 cagcgttttt tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg
    50101 ctggtgatga cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga
    50161 tagaaaaatg agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg
    50221 gggtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    50281 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat
    50341 gaaaagacgc atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg
    50401 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct
    50461 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    50521 ggcgctgccg ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg
    50581 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg
    50641 cttcggcgct gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    50701 caacgaacat cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca
    50761 aaaaattaat tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc
    50821 aaagtctcca ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc
    50881 aaatccgcct tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag
    50941 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    51001 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    51061 gaatcgctgt aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc
    51121 tgccagcgta agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg
    51181 gctgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg
    51241 ggccatcgtc cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg
    51301 cggaaatgac cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg
    51361 cggcagttcg cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg
    51421 cccatctttg ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc
    51481 ttttgcgccc cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat
    51541 ttgccagatc ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat
    51601 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    51661 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    51721 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    51781 cggatgtggt gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    51841 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    51901 tcccaccgga gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc
    51961 acggagttga cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg
    52021 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag
    52081 aacgcacgat gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    52141 tgacgaaaga cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct
    52201 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga
    52261 acgaaatggc ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact
    52321 caatggatcg tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt
    52381 atatcaccca gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg
    52441 cgccggagat tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata
    52501 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    52561 aagtgcagca ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc
    52621 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca
    52681 acaagattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    52741 tcaccagcgc caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat
    52801 tgagtatgga aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga
    52861 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg
    52921 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    52981 cgcaaaatgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    53041 accgcgccac ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    53101 cggcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct
    53161 gggatgcagg tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc
    53221 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc
    53281 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa
    53341 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    53401 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    53461 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    53521 aatgcgatat tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    53581 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    53641 tggagcaggc aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg
    53701 tcatcaacac tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg
    53761 aaccaggctg gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg
    53821 gcgggaaaac cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    53881 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    53941 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    54001 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga
    54061 cgccgcctcc gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg
    54121 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc
    54181 acgaggtggg aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa
    54241 ggcgcttcgg cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt
    54301 gaacgcgtta tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag
    54361 cctgataagc gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca
    54421 accattcgcg cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt
    54481 ccagcaaatc catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat
    54541 cgccttccag cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa
    54601 agtaagacgg tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa
    54661 cctcttcttt tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc
    54721 ctttcgaaaa acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca
    54781 ggtgatcatc cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt
    54841 cataaacacg ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc
    54901 gggaacgggc tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg
    54961 tgggatctat gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg
    55021 tgaattgcat gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta
    55081 aaagtcaata ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg
    55141 ctatgatcag cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc
    55201 gatagccaac cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta
    55261 agtaagatgg ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct
    55321 cactacttgc gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca
    55381 acgccttcgc ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa
    55441 attatcttga gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg
    55501 caccgatctc tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta
    55561 tcacacgtta cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg
    55621 tagaagtcca cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac
    55681 tgattcatac cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt
    55741 acatgcagga aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc
    55801 gcggggaaga gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat
    55861 atgcgctgag tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga
    55921 tgttcttttt ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat
    55981 tactgctgct gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc
    56041 gagaaattca ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc
    56101 aggaacagat caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc
    56161 agccatacat tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc
    56221 acgtagtgcg tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac
    56281 agcactggct gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt
    56341 tattctggat cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc
    56401 agaccattga agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata
    56461 cattgcgtat tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga
    56521 aaaccaattc acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct
    56581 cattaccatt accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta
    56641 atcgccagct gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag
    56701 cgattcaaaa atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag
    56761 cggatttatg ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg
    56821 gcaacagtaa agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg
    56881 tgaatgtgtt attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca
    56941 ccgcgccgtt tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc
    57001 ccggcggatt cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt
    57061 cggcatcact ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac
    57121 aaatgctgat gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg
    57181 ccaatggtac gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct
    57241 atctcagcac cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac
    57301 aggcctggcg tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg
    57361 aaagctgcct gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt
    57421 gcgcggcagg ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg
    57481 catatcaaca ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg
    57541 ctcgacctcg ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg
    57601 aaaaacgggg ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta
    57661 cagcatacgc taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc
    57721 tgtgcgatga ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt
    57781 cttgaggcac tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac
    57841 aacctggcgg taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc
    57901 acccacacat ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct
    57961 acgggtctga aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct
    58021 gccgcgcaat ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc
    58081 gagaaacagt atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta
    58141 atgtgaagga gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct
    58201 atgggctgcc cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa
    58261 ggtgcattac aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct
    58321 cactctgcgc cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg
    58381 tcgccccgcc agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg
    58441 cgaaaaaatg gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg
    58501 cccggacaaa aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact
    58561 gtcacctgca agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga
    58621 aaattttgcc gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga
    58681 gaaccacgat tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag
    58741 cctgttaacc gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg
    58801 tccgatgaat ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg
    58861 cgtgcagggc gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt
    58921 ggtgatcacc attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg
    58981 tgataccctg gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg
    59041 cctgtttatt cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca
    59101 ggtaatgcct gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga
    59161 aaccatcaaa accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta
    59221 tcacgaagaa gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc
    59281 gcgtgaacgt tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct
    59341 ggcggaagat ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa
    59401 tgcgatggat attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta
    59461 atgagtatgt ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct
    59521 gcgcttacca gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat
    59581 ccgataaaag cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag
    59641 atcgcgccac gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt
    59701 ttctttcgtt atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc
    59761 tgagtcaaag ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc
    59821 ctgaccatca ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
    59881 catcacgtta tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga
    59941 accaggggca attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg
    60001 ttgctctcca tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc
    60061 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    60121 gtagatagtt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    60181 cacgctaata cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac
    60241 caataacgaa accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg
    60301 ccagacgata ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc
    60361 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt
    60421 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    60481 ccccgtccag ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    60541 caaaaaggcc agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag
    60601 gcccgtaaac ggcagggtga tgataaacat cggggttagc aatgatacta acggcagcaa
    60661 ccccgtggcg attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    60721 cgcgaggatc accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt
    60781 tgcaaaaaag agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag
    60841 taaaagcacc agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt
    60901 atgccatact gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct
    60961 ttgttgtatc cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca
    61021 aatcgccttt tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct
    61081 catccctgtc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    61141 agagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    61201 aatccaactg tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg
    61261 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    61321 gttggttatc cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct
    61381 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt
    61441 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    61501 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    61561 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    61621 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    61681 tggctaagga gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct
    61741 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    61801 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    61861 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    61921 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    61981 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc
    62041 ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc
    62101 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    62161 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    62221 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    62281 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    62341 atgttctcga tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    62401 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    62461 accacccttt ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac
    62521 gatgacggcg tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    62581 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    62641 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    62701 tcttatccaa tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg
    62761 actaaggtta tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg
    62821 actgccgatc aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact
    62881 gagcgtggca tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg
    62941 tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg
    63001 caggcttatc tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat
    63061 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact
    63121 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc
    63181 gatccgcgta acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg
    63241 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    63301 gcggctggtc cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag
    63361 aaagcaaaac gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg
    63421 cgcccgcgtt accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt
    63481 gccaagctcc agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa
    63541 tcccgtgccg ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt
    63601 acgttgttcc ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg
    63661 ctccgttccg ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat
    63721 attcgccacc gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta
    63781 aagcgcggtt tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac
    63841 tgcattaaga tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa
    63901 ctgctcaatg atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc
    63961 atcctgctcg ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc
    64021 gtgacttacc cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc
    64081 catatggtta aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg
    64141 cggcggaata atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg
    64201 gttctggata cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt
    64261 atagcggaac agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca
    64321 gatattgggc gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac
    64381 gcgcacttcc gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg
    64441 ttgcgcccaa cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag
    64501 ctcacggtag atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag
    64561 ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg
    64621 gaggctcggc aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa
    64681 cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa
    64741 tcgcctcatg ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga
    64801 atgtaacgcg gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg
    64861 tcgatggagc gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg
    64921 cgggagagcg gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg
    64981 gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac
    65041 ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga
    65101 cgacgcagca ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg
    65161 tagtcgtcag cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc
    65221 accataatga tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca
    65281 tcttcaccag gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg
    65341 cgatccatct gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa
    65401 cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct
    65461 tgcattgttt gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc
    65521 tcacgttcgt caccagctaa atctggtata aatttcagcc taaattgtta caaagcatat
    65581 taaacagcag cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga
    65641 tcacgttata cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac
    65701 gcccctggtt acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg
    65761 tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag
    65821 cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg
    65881 ctatctcact aaatgcctgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg
    65941 caaagtattt tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt
    66001 ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc
    66061 gatggcccgc gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc
    66121 cggggaagcg aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt
    66181 aacctcctgc cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt
    66241 tgatccgaaa agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg
    66301 tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa
    66361 taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca
    66421 gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc
    66481 gatcctcaaa tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa
    66541 cgccacccta agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg
    66601 cacccgcggg caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag
    66661 cgatccgtca cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt
    66721 ggcagatacc aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga
    66781 agatgccgcg ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc
    66841 tacggtggtg agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca
    66901 cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga
    66961 aggcgtactc cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag
    67021 ctattgcgag caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga
    67081 tagctggcgc aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga
    67141 aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc
    67201 ccgtaacctg cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct
    67261 cgatccgggt ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt
    67321 ggcgaccgat accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc
    67381 tgccttgtgt gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg
    67441 cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa
    67501 agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga
    67561 gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga
    67621 tccgctggcg gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca
    67681 tgacgtcagc cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa
    67741 cgccgttggc gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct
    67801 gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa
    67861 ccgtcagcaa ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg
    67921 cttcctgcgc attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc
    67981 ctatccggtg gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg
    68041 taacagcagc gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt
    68101 gccgacagta actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga
    68161 atttaaaacc gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat
    68221 gatcctcgaa ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt
    68281 gcatcaggac ggcctggttc acatctcttc attatcgaac aagtttgtgg aagatccgca
    68341 taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg
    68401 taaacgtatc gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg
    68461 cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga
    68521 agcgcagcct gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa
    68581 acgttaaacg cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg
    68641 caacgaacgc tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac
    68701 agccaaactc acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa
    68761 ttaaacatta gccagtccgg gtaactcact attcgaatta tattttcgct gcgatataac
    68821 cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta
    68881 agtattgctg atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct
    68941 ttgttggcaa atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag
    69001 ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact
    69061 ggcttttccc gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta
    69121 cctggctcct cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa
    69181 acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg
    69241 gtttcctgtt aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa
    69301 tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg
    69361 taactgggct ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca
    69421 ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc
    69481 gctcgatgag caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa
    69541 cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact
    69601 cggcattccc tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg
    69661 tattgaaatt gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac
    69721 ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa
    69781 tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt
    69841 gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg
    69901 cgatatctac agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc
    69961 ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg
    70021 cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac
    70081 cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt
    70141 gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttaa
    70201 cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt
    70261 cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct
    70321 gccactggtg ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg
    70381 ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg
    70441 gaaatccttt gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc
    70501 acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc
    70561 ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg
    70621 tgccctggcg gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct
    70681 gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt
    70741 ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt
    70801 cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag
    70861 cttctcgttg agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag
    70921 ccgggtgata acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac
    70981 ggttggcctg tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct
    71041 ctacaccgca gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga
    71101 agagctgttc agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag
    71161 cgtactgatg aaccccattg aagctagcaa aggcaacggt gaaatgggta ccggggcgat
    71221 gggcgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt
    71281 cctgctgtat gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg
    71341 ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt
    71401 ctatcaggtc gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt
    71461 aatcctgttt aacatcgtag ctatcggtct gctgcgccgc gcgcgtagcc gggtggatat
    71521 cgaactgctg gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga
    71581 ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg
    71641 gaagcgaccc agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg
    71701 caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc
    71761 tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg
    71821 cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt
    71881 tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa
    71941 gcgtctcgca gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg
    72001 gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga
    72061 tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca
    72121 tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgaggag
    72181 ctttattgat gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga
    72241 acaaggacca ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat
    72301 cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata
    72361 caaaacgttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc
    72421 ctattcgctg tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc
    72481 ctccgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg
    72541 acgtatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct
    72601 ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg
    72661 gctgtttaat tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg
    72721 cgttgccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg
    72781 gcaggagggg gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt
    72841 ccctcttgca gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcggcgag
    72901 tagactggcc ccctgaatct ccagacaacc agtat
//
LOCUS       DAJTNV010000004        72002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNV010000004 DAJTNV010000000
VERSION     DAJTNV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(1325..2920)
                     /locus_tag="OO199_000233"
     CDS             complement(1325..2920)
                     /locus_tag="OO199_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5534420.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGSTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3275..5446)
                     /gene="glcB"
                     /locus_tag="OO199_000234"
     CDS             complement(3275..5446)
                     /gene="glcB"
                     /locus_tag="OO199_000234"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5534421.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5468..5872)
                     /locus_tag="OO199_000235"
     CDS             complement(5468..5872)
                     /locus_tag="OO199_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5534422.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5877..7096)
                     /gene="glcF"
                     /locus_tag="OO199_000236"
                     /pseudo
     CDS             complement(5877..7096)
                     /gene="glcF"
                     /locus_tag="OO199_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7107..8159)
                     /gene="glcE"
                     /locus_tag="OO199_000237"
     CDS             complement(7107..8159)
                     /gene="glcE"
                     /locus_tag="OO199_000237"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5534423.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8159..9658)
                     /gene="glcD"
                     /locus_tag="OO199_000238"
                     /pseudo
     CDS             complement(8159..9658)
                     /gene="glcD"
                     /locus_tag="OO199_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9909..10679
                     /gene="glcC"
                     /locus_tag="OO199_000239"
     CDS             9909..10679
                     /gene="glcC"
                     /locus_tag="OO199_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5534424.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10803..11944)
                     /locus_tag="OO199_000240"
                     /pseudo
     CDS             complement(10803..11944)
                     /locus_tag="OO199_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12306..14036
                     /locus_tag="OO199_000241"
     CDS             12306..14036
                     /locus_tag="OO199_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5534425.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14033..14947
                     /locus_tag="OO199_000242"
     CDS             14033..14947
                     /locus_tag="OO199_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5534426.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14980..15228
                     /locus_tag="OO199_000243"
     CDS             14980..15228
                     /locus_tag="OO199_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5534427.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15228..16399
                     /locus_tag="OO199_000244"
                     /pseudo
     CDS             15228..16399
                     /locus_tag="OO199_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16435..17514)
                     /gene="lptG"
                     /locus_tag="OO199_000245"
     CDS             complement(16435..17514)
                     /gene="lptG"
                     /locus_tag="OO199_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5534428.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17511..18581)
                     /gene="lptF"
                     /locus_tag="OO199_000246"
     CDS             complement(17511..18581)
                     /gene="lptF"
                     /locus_tag="OO199_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5534429.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18612..19172)
                     /locus_tag="OO199_000247"
     CDS             complement(18612..19172)
                     /locus_tag="OO199_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5534430.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19184..20008)
                     /locus_tag="OO199_000248"
     CDS             complement(19184..20008)
                     /locus_tag="OO199_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534431.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFFAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20008..21357)
                     /locus_tag="OO199_000249"
     CDS             complement(20008..21357)
                     /locus_tag="OO199_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5534432.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21403..22161)
                     /locus_tag="OO199_000250"
     CDS             complement(21403..22161)
                     /locus_tag="OO199_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534433.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22193..22906)
                     /locus_tag="OO199_000251"
     CDS             complement(22193..22906)
                     /locus_tag="OO199_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5534434.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23080..23772
                     /locus_tag="OO199_000252"
     CDS             23080..23772
                     /locus_tag="OO199_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5534435.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23821..25320)
                     /gene="pitB"
                     /locus_tag="OO199_000253"
     CDS             complement(23821..25320)
                     /gene="pitB"
                     /locus_tag="OO199_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5534436.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25612..27471)
                     /gene="gss"
                     /locus_tag="OO199_000254"
     CDS             complement(25612..27471)
                     /gene="gss"
                     /locus_tag="OO199_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5534437.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27676..28542
                     /gene="yghU"
                     /locus_tag="OO199_000255"
     CDS             27676..28542
                     /gene="yghU"
                     /locus_tag="OO199_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5534438.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28665..28913)
                     /gene="hybG"
                     /locus_tag="OO199_000256"
     CDS             complement(28665..28913)
                     /gene="hybG"
                     /locus_tag="OO199_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5534439.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29047..29388)
                     /gene="hypA"
                     /locus_tag="OO199_000257"
     CDS             complement(29047..29388)
                     /gene="hypA"
                     /locus_tag="OO199_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5534440.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29381..29869)
                     /gene="hybE"
                     /locus_tag="OO199_000258"
     CDS             complement(29381..29869)
                     /gene="hybE"
                     /locus_tag="OO199_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5534441.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29862..30356)
                     /locus_tag="OO199_000259"
     CDS             complement(29862..30356)
                     /locus_tag="OO199_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5534442.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30356..32059)
                     /gene="hybC"
                     /locus_tag="OO199_000260"
     CDS             complement(30356..32059)
                     /gene="hybC"
                     /locus_tag="OO199_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5534443.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32056..33234)
                     /gene="hybB"
                     /locus_tag="OO199_000261"
     CDS             complement(32056..33234)
                     /gene="hybB"
                     /locus_tag="OO199_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5534444.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33224..34210)
                     /gene="hybA"
                     /locus_tag="OO199_000262"
     CDS             complement(33224..34210)
                     /gene="hybA"
                     /locus_tag="OO199_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5534445.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34213..35331)
                     /gene="hybO"
                     /locus_tag="OO199_000263"
     CDS             complement(34213..35331)
                     /gene="hybO"
                     /locus_tag="OO199_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5534446.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35520..35807)
                     /locus_tag="OO199_000264"
     CDS             complement(35520..35807)
                     /locus_tag="OO199_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5534447.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35926..36813)
                     /locus_tag="OO199_000265"
     CDS             complement(35926..36813)
                     /locus_tag="OO199_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5534448.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36970..38010
                     /gene="gpr"
                     /locus_tag="OO199_000266"
     CDS             36970..38010
                     /gene="gpr"
                     /locus_tag="OO199_000266"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5534449.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38050..38544)
                     /locus_tag="OO199_000267"
     CDS             complement(38050..38544)
                     /locus_tag="OO199_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5534450.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38735..39619
                     /gene="yghA"
                     /locus_tag="OO199_000268"
     CDS             38735..39619
                     /gene="yghA"
                     /locus_tag="OO199_000268"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5534451.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39891..40316)
                     /gene="exbD"
                     /locus_tag="OO199_000269"
     CDS             complement(39891..40316)
                     /gene="exbD"
                     /locus_tag="OO199_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5534452.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40323..41057)
                     /gene="exbB"
                     /locus_tag="OO199_000270"
     CDS             complement(40323..41057)
                     /gene="exbB"
                     /locus_tag="OO199_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5534453.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41309..42496
                     /gene="metC"
                     /locus_tag="OO199_000271"
     CDS             41309..42496
                     /gene="metC"
                     /locus_tag="OO199_000271"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5534454.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42636..43295
                     /locus_tag="OO199_000272"
     CDS             42636..43295
                     /locus_tag="OO199_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5534455.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43335..44234)
                     /gene="yqhC"
                     /locus_tag="OO199_000273"
     CDS             complement(43335..44234)
                     /gene="yqhC"
                     /locus_tag="OO199_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5534456.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44428..45591
                     /gene="yqhD"
                     /locus_tag="OO199_000274"
     CDS             44428..45591
                     /gene="yqhD"
                     /locus_tag="OO199_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5534457.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45696..46523
                     /gene="dkgA"
                     /locus_tag="OO199_000275"
     CDS             45696..46523
                     /gene="dkgA"
                     /locus_tag="OO199_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5534458.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46723..47649
                     /locus_tag="OO199_000276"
     CDS             46723..47649
                     /locus_tag="OO199_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5534459.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47700..47957
                     /gene="yqhH"
                     /locus_tag="OO199_000277"
     CDS             47700..47957
                     /gene="yqhH"
                     /locus_tag="OO199_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5534460.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48000..50219)
                     /locus_tag="OO199_000278"
     CDS             complement(48000..50219)
                     /locus_tag="OO199_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5534461.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50330..51742)
                     /gene="ftsP"
                     /locus_tag="OO199_000279"
     CDS             complement(50330..51742)
                     /gene="ftsP"
                     /locus_tag="OO199_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5534462.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51817..52554)
                     /gene="plsC"
                     /locus_tag="OO199_000280"
                     /gene_synonym="parF"
     CDS             complement(51817..52554)
                     /gene="plsC"
                     /locus_tag="OO199_000280"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5534463.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52788..55046)
                     /gene="parC"
                     /locus_tag="OO199_000281"
     CDS             complement(52788..55046)
                     /gene="parC"
                     /locus_tag="OO199_000281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5534464.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55592..56074)
                     /locus_tag="OO199_000282"
     CDS             complement(55592..56074)
                     /locus_tag="OO199_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5534465.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56127..56519)
                     /gene="ygiW"
                     /locus_tag="OO199_000283"
     CDS             complement(56127..56519)
                     /gene="ygiW"
                     /locus_tag="OO199_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5534466.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56671..57330
                     /gene="qseB"
                     /locus_tag="OO199_000284"
     CDS             56671..57330
                     /gene="qseB"
                     /locus_tag="OO199_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5534467.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57327..58601
                     /gene="qseC"
                     /locus_tag="OO199_000285"
     CDS             57327..58601
                     /gene="qseC"
                     /locus_tag="OO199_000285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5534468.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58647..58979)
                     /locus_tag="OO199_000286"
     CDS             complement(58647..58979)
                     /locus_tag="OO199_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5534469.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59298..59879
                     /gene="mdaB"
                     /locus_tag="OO199_000287"
     CDS             59298..59879
                     /gene="mdaB"
                     /locus_tag="OO199_000287"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5534470.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59910..60224
                     /locus_tag="OO199_000288"
     CDS             59910..60224
                     /locus_tag="OO199_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5534471.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60272..62164)
                     /gene="parE"
                     /locus_tag="OO199_000289"
     CDS             complement(60272..62164)
                     /gene="parE"
                     /locus_tag="OO199_000289"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5534472.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62193..62774)
                     /gene="yqiA"
                     /locus_tag="OO199_000290"
     CDS             complement(62193..62774)
                     /gene="yqiA"
                     /locus_tag="OO199_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5534473.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62774..63601)
                     /gene="cpdA"
                     /locus_tag="OO199_000291"
     CDS             complement(62774..63601)
                     /gene="cpdA"
                     /locus_tag="OO199_000291"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5534474.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63626..64048)
                     /locus_tag="OO199_000292"
     CDS             complement(63626..64048)
                     /locus_tag="OO199_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5534475.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64049..64678)
                     /gene="nudF"
                     /locus_tag="OO199_000293"
     CDS             complement(64049..64678)
                     /gene="nudF"
                     /locus_tag="OO199_000293"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5534476.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64883..66364
                     /gene="tolC"
                     /locus_tag="OO199_000294"
     CDS             64883..66364
                     /gene="tolC"
                     /locus_tag="OO199_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5534477.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66512..67183
                     /locus_tag="OO199_000295"
     CDS             66512..67183
                     /locus_tag="OO199_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5534478.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67189..68349
                     /locus_tag="OO199_000296"
     CDS             67189..68349
                     /locus_tag="OO199_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5534479.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68387..69175)
                     /gene="ygiD"
                     /locus_tag="OO199_000297"
     CDS             complement(68387..69175)
                     /gene="ygiD"
                     /locus_tag="OO199_000297"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5534480.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69318..70091
                     /gene="zupT"
                     /locus_tag="OO199_000298"
     CDS             69318..70091
                     /gene="zupT"
                     /locus_tag="OO199_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5534481.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70149..70319)
                     /locus_tag="OO199_000299"
     CDS             complement(70149..70319)
                     /locus_tag="OO199_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534482.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70580..71233)
                     /gene="ribB"
                     /locus_tag="OO199_000300"
     CDS             complement(70580..71233)
                     /gene="ribB"
                     /locus_tag="OO199_000300"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5534483.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71337..71485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71607..71897
                     /gene="ubiK"
                     /locus_tag="OO199_000301"
     CDS             71607..71897
                     /gene="ubiK"
                     /locus_tag="OO199_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5534484.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18004 a        19155 c        17733 g        17110 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta
      481 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa
      541 tgattttaat gattttaatg attttaatga ttttttcatt tattcacacc taataaaagt
      601 attcaaattc agatgatttt tctgctcaac agacacaaac aggcaataat actctccaaa
      661 gcgaacaata tttggcatta cgatttaatg cttcataatc accaaattat ttaaccataa
      721 attttcttat ttctaacaaa atgattggtg tgttgttgga gttaaaatac atacataata
      781 ttaacatctg ccaatatttc actttccgca agacggatga tttcgaatcc tcatccacgt
      841 taccctatcc caaatatctt tattaatagg cagtaacgaa cactgttata gcaagattaa
      901 cttattaacc taacggcatc ttttatgtaa caagtggaga taaattgctt aaaattgatc
      961 ctcgacaaat cttaaaagtt ctcattattc tgcaaaatca gctttgcgaa taatcttatg
     1021 tttttgggag ttttgggaat tttgatctac tcattgttct agtgaaaaat ataaataaga
     1081 gaaaaaacgc cattcgtcat gaaagcaaca gatgaagcaa tgtaataaca tactaaaatt
     1141 gatctcatga aagtgggtac tttataacca gcacagaagc caagttgtat aaatattaaa
     1201 caattcgcat cgtaaaacgg agactacgta taaagaagcc aatcgtaaaa acggttccct
     1261 ggcgagataa acgacagaca aatggaaagc cgggtgatat ttaccttcat ccggctttat
     1321 tgatttacga gactaacatc ccggtaaaca catacgcctg cagcagggtg ataacgccga
     1381 taacgctggc aaaaatcaga ctgtgcttca cggtgtagcg gaacagttca gattctcggc
     1441 ccaccatgcc cgtcgcggcg caggccacgg cgatagattg cggggagatc atcttgccag
     1501 ttacgccgcc gctggtattt gctgccacca gcagagtgtc agagacgttg atttgctgcg
     1561 ccgtggtcga ttgcagtgaa ccaaacaggg cgtttgaaga agtgtccgac ccggtaagga
     1621 atacgcccag ccagccgaga aacggtgaga aaaacgggaa catcacgcct gtacctgcca
     1681 gtaccagcgc cagcgtggtg gacatgccgg aatagttggt gacgaaggcg aatgccagca
     1741 ccatgccaat cgacaatatc ggccacttca gactaattag catttcggca aagacgccaa
     1801 tccctttctt gatgcccacg ccgaggatga agatagagat aatcgccgca ataaaaatag
     1861 cggtgctgcc agccgagaga gggtcgaatt taaacaccgc atccattggc gttggttggg
     1921 cgacaatggg tgccgctttc aacacttgtt gatgcaaatg agggatctgg aaattaatca
     1981 ccagtgaata aaacgcgccg cccggagcaa ataacgcttt aaacggcttc atggtccaga
     2041 tggtgaccag caccgttaag attaaaaacg gtgaccacgc acgaatgatt tgccccagac
     2101 tatattctga aggcacggga ccgccagaag atggcttatt taccaccatc gcacctgcgg
     2161 attgccccat gctgattgcc gtttcggtat ttttcggccg ccagacttta agaaataaag
     2221 cgagtgagac gatactcacc agcgccgaag taatatccgg cagttccgga ccaatatagt
     2281 tagaggtaaa gaactgagtg acagcgaagc ttcccccagc aaccagcgcc gctggccacg
     2341 tctctttcac ccctttccag ccgtccatca ttgctaccag ccagaacggc acaagaaccg
     2401 acaggaacgg taactgacgt cccgccattg cgccaatgtg gaacggatcg attcccgtta
     2461 cctgaccggc gaccagaatc ggcacgccca acgcaccaaa cgccaccgtc gcaccaaagc
     2521 cagcagctcc ttccagcaac gcaccaaagg agaaaccaat cagtaacacc tgcaaacgct
     2581 gatcgtcggt gatggagata accaagctgc ggataatatc gaactgcccg ctggcaacgg
     2641 ttaatttata caggaacacc gccgcgacaa taatccacgc aattggccat aatccataaa
     2701 taaagccata gcccgcagca gcaaatgcca tatcaatcgg cattttaaag gcgaatatcg
     2761 caatcaggat agataatata agggttattg ctccagcgac atgtcctttc agacgtaata
     2821 ccgcgagtgc aacgaagaag aatattatcg ggatcagggc gaccagagcg gatagcccca
     2881 gtcctcccat cggcatatac atttgggtcc aggtaaccat attgttcagt ctcttattat
     2941 ctttatatgc ttgatatact taaggttgta ataagcaaaa gaggactgaa ctgtaaaata
     3001 taggcgttat actttacagc aacagtacgc cgctaacgca attgctacct ctggcataac
     3061 aagtatatcg ggtaagggtt tctgttccgc acacgcaggc gcagagtatc gttaagatgt
     3121 ccatattgtt gttttaggcc cgctagtaat gcgctacggg tatttaatat tgttaaaccc
     3181 tgataatcgc tccggttatt tccgggataa atgtactacc gcagttacta tcatagcccc
     3241 gacaataaaa cttgccgggg cttttttgac gctattaatg actttctttt tcgcgtaaac
     3301 gccaggcgtg taataacggt tcggtataac cgtttggctg tttcacgccg aggaagatta
     3361 aatcgctggc agctttaaaa gcacacgagt tagcgaaatt ccccgccatc ggacgataag
     3421 ccggatcgcc agcattttgc tgatcaacca ctttcgccat attctccagc gacgcctgca
     3481 cctgttcttt ggtcagaata ccgtgacgta accagttggc gatatgctgg ctggagatac
     3541 gcagcgttgc gcggtcttcc atcagcgcca cgttgtgaat atccggcact tttgaacaac
     3601 caatcccctg ctccacccaa cgcaccacgt accccagaat cccctgcacg ttgttatcca
     3661 gctcttgttg gatctcttgc gccgaccagt tagcgttttc agccaccgga atagtcagca
     3721 gatcgtccag cagcggttca aattcagcac tgaactcggt ctgggcaatg ttggcttgca
     3781 cgctctgtac gttggtatgg tggtagtgca gcgcatggag cgtagcagcg gttggtgacg
     3841 gaacccaggc tgtgtttgcc ccggcacgca gttggtcgcc cttctggctg tacatgtctg
     3901 ccatcaggtc cggcattgcc cacatgcctt taccgatttg cgctttaccg cgcagcccac
     3961 agaacagacc ggaaagcacg ttattacgtt cgtaggcttt gatccacggc gtcgatttca
     4021 tctggttttt acgcagcatc gggccagctt ccatcaccga atgcatttca tcgccggtac
     4081 ggtcgaggaa accggtattg atgaacgcca cgcggttgcg cgcctgagcg atacagctac
     4141 gcaagttcag cgaggtccgg cgttcttcat ccataatgcc cattttcagg gtattcggtg
     4201 ccataccgag cattgtctca atgcgggtaa acagtttgtt ggcgaacgcc acttcctgcg
     4261 gaccgtgcat tttcggtttc acaatataga cgctgccagt gcgcgagttt ttctgcactt
     4321 ttaaatcata gagggcaatc gcgccagtca tgacgccatc aagaatacct tccgggattt
     4381 cattgccttc gctgtcccaa atcacaggaa tggtcatcaa atgacccacg ttgcggataa
     4441 acagcaacga gcgtccgtgc agagaaattt cagagccatc ggcggcggtg tactgacgat
     4501 cgtcattcag tttacgtacg atttgccgac cgtttttctc cattttctct tgcagagtcc
     4561 cctgcatcag gcccagcaga ttgcggtaca gcaggatttt atcttccgca tcaaccgccg
     4621 cgaccgaatc ttcgcagtcg agaatggtac tgatagcagc ttcgacgata acatcgttga
     4681 tgtgcgccgg atcgtctttg ccaatccgcc cgttggcatc aatttgcagt tcaatatgca
     4741 ggccgttatt tttcagcaaa atgcaggtcg gcgcagcggc atcgccacgg taaccgacaa
     4801 actgtgctgg agtacgtaac gtggtttctt taccattttt caactggatg cgtaattgtt
     4861 tatcgaccac cttaaacgcc accacatcct gatagctgcc gttttccagc ggtagtgatt
     4921 catcgaggaa acgtcgaacc caggcgataa cctgctcacc gcgctgcgga tcgtagccac
     4981 tgaccatcgc cccttcctgc gggatgatgt cgctgccgta taacgcatcg tacagtgagc
     5041 cccagcgagc gttcgccgcg ttcagcgcgt agcgggcgtt cattgccgga accaccagct
     5101 gtggccccgc ctgactggtg atttcgctgt caatgcccgt ggtttccacc gtcacgcgct
     5161 ccggttgcgg caccaggtag cccagttcac gaaggaaaga tttataggcc gctttatctt
     5221 ttaccggccc cggattgctg cgatgccact catcaagcgc cgcctgaatg cgatcgcgtt
     5281 ctgccagcaa ctgacgattt tctggtgcca gatcgtgaac gatctcatca acattgtgcc
     5341 agaatgcagc agcgtccagt tccacgcccg gcaaaacttc tccatccaca aaacgtttaa
     5401 aattggcgtc aatgcgtaaa cggccctggg ttatggtttg actcattgtt tctctcctcg
     5461 ttttcgctta tttcgccaac accgctgctg ccactttcgc gacctgcgca tcctgtgctc
     5521 cggttaaacc agaaacgccc acggcaccaa taatttgccc atcaacaaca accggtacgc
     5581 cgccttccag cgacgtcagt aacggcgcag tcacgaacgc ggtacgtccg ttgttcacca
     5641 tctcttcata gcccttagtt tcacgacgcc ccagcgcggc ggtacgcgct ttctcctggg
     5701 agatgtaagc cgcaatcggc gcgcaatcgt ccatgcgact taacgccagc agatgaccgc
     5761 cgtcatcggc aacagcaatg gaaacagacc agttattttt ctgcgcctct tcctgacctg
     5821 cggcaataat tgcactcgcc atttgctggc taagaatgac tttagttttc atttttttat
     5881 tccttttcaa gggcttgttc tacaatttca atccagtgac gcacagaggt acgaccggcg
     5941 ctcgccagat gcgtctggca accaatgttg gcggtgacga tcatttccgg tttgccgctt
     6001 tccagcgcat tcattttgtt atcccgcagc tggcgtgcca gatcgggatg cgttaacgca
     6061 tatgttcccg ctgaaccgca gcacagatgg ctgtcgggaa cgtccgttaa ggtaaatcca
     6121 agacgaagta acactttttc cacttcgccg ttcagctttt gcgcatgttg tagggtacac
     6181 gggcagtgga aggccagttt tttatcgccg cgaattgcca gtttttccag cggttcctcg
     6241 cgcagaagtt cgactaaatc gaccgccagt tcactgacct gacgcgcttt atcggcatat
     6301 aacgcatcgt ttttcagcat ctgcccatac tctttgacga acgcgccgca accgctggcg
     6361 gtttgcaaaa ttgcctcggc acctgcttca atcgcgggcc accaggcatc aatattattg
     6421 cgcgcccgtg ccaacccttt ctcctgcgca ttaagatgat agtccaccgc gccacaacag
     6481 cctgcttcgt tagctgacat gacgctgatc cccagacgat ccagcactcg cgcagttgcc
     6541 gcgttggtgt tgggcgaaag cgtaggctgg gcgcagcctt ccagcattaa aacccgacgc
     6601 ttatggcgca gcggcggacg cggtttagct ttcaccgttt cagcaggcag ttttgctctg
     6661 acctgttccg gtaaaaacgg tcgcagcacc agccctacct gcgtcagcgc acggaagacc
     6721 gccggacgcg gcactacctg gcgcaatcct tcgcgcagca ttcgctccgg cagtgggcgt
     6781 ttcactttct gctcgacaat atcacgcccg atatccagca aattgtgata gcgcacacca
     6841 gaaggacagg tggtttcaca attgcggcaa gtgaggcagc gatcgagatg ctcctgtgtt
     6901 ttaagcgtga cttcgttgcc ttccagcacc tgtttaatca gatagatgcg cccgcgcggc
     6961 ccgtccagtt catcgcccag aagctgatag gttgggcagg ttgcggtaca aaatccgcag
     7021 tgaacacagg cgcacaggat gctgtcggct tccagcgcgc gttctgccgc atctcttcgg
     7081 ttaatcgggt ttgcatagcc tgctcctcaa agttccgcgt acatgcgacc ggggttaaac
     7141 acgccgcaag ggtcgagctg ctgtttaagc tgctggtgat agcggaataa aggagccgat
     7201 agcggggcaa agccaccatc tccggcacta aagcgggtcg catgaccgcc agcgttgcgg
     7261 gcgatgcgat ggatttgatt gtcatcggct gtcgatttca gccagcgtaa cgccccgccc
     7321 cagtcgatca gttgctcgcc gggtaaatcc atcatcggcg catcgctggg taatgaaatg
     7381 cgccataagg tacctggtaa cgagaagaac ggcagttgtt gttcacgcaa ttgctgccag
     7441 aactgacccg caacctcttc gccacccagc agttcacgcg ctgcttttac cgatccttcg
     7501 ccgccctcaa ggcggatcca caacgcattg tcgaagtaac ataagccact aatgggtaat
     7561 ggctggagtt gccactcggc gatttcactc atggcttctt gcaggctgat ttcccgacgc
     7621 aggctcagag aggcacgcgg tcgcggtaac actttcattg agatttcagt gagcacgcca
     7681 agacaaccat agcttccggc cattaaccgt gagagatcgt atccggcaac gtttttcatc
     7741 acttcgccac caaaacgcag atgttttcca gtgccggtaa tgatgcgcgt gccgaggaca
     7801 aaatcgcgga ccgaaccgct ccacgggcga cgcggccccg ccagcccgca ggcgaccatc
     7861 ccgccccagg tggcttcttc accataatgc ggcggctcgc agggaagcat ttgcccggcg
     7921 ctctccagcg ccgcttcaat cgccaccagc ggcgttccgg cacgagcggt tatcaccagc
     7981 tcggtcgggt cgtaattaac aatgccgcga tgacaacgca catccagcgt ttgcccggtg
     8041 actgggcgac ctaaaaaggc tttgctattg ctgccctgaa tcaccagcgg cgttttatcg
     8101 ctaattgcct gattcacctg ctccagcagc gcctgactgt aatcacactc gcgtagcatc
     8161 aaaagcgctc cagttcagga aaaggtaaat gaccgtgatg cacatgcatg gcaccaaatt
     8221 cagcacagcg gtgtagcgtg ggaatgtttt tcccagggtt cagcaaacca tcggggtcaa
     8281 acgccgcctt gaccgcatgg aaggtcgtga tttcatcgct gttgaactgg gcgcacattt
     8341 gattgatttt ttctcgcccg atgccatgtt ccccactgat gctgccgcca acttcaacgc
     8401 agagttcgag gatcttcccg cccagctctt ccgcgcgggc aaattcacca ggttcgttgg
     8461 catcgaaaag gattaacggg tgcatgttgc catctccggc atgaaagacg ttggcaacac
     8521 gtaaatcata ttgctgcgat aaacgggcaa tgccttccag tacgccaggc agggcgcgac
     8581 gcgggatggt gccatccatg cagtagtaat ccggggagat acgtcctacc gccgggaacg
     8641 catttttgcg accggcccag aaacgtacgc gctctgcttc gtcctgtgcc aaacggacgt
     8701 cagtcgcgcc cgctttcaac aagatgtagt taacccgctc gcagtcttcc tgtacgtcag
     8761 actccacgcc gtccagttcg cataacaaaa tcgcttcggc gtcgacggga taaccggcat
     8821 gaataaaatc ttccgccgcg cggatcgaca ggttatccat catctccagc ccgccgggga
     8881 taatgccatt ggcgatgatg tcaccaaccg caagtccggc tttttctacc gagtcaaagc
     8941 tggctaacag aacccgcgcc acgggcggct tcggcagcag ttttaccgtc acttcggtgg
     9001 tcacgccgag cataccttcc gatccggtga acagcgccag caggtcaaaa ccaggtgaat
     9061 ccagcgcgtc cgatcccagc gtcagcgcct cgccgtccag cgtttgcact tcaattttca
     9121 gcaggttatg tacggtcaga ccatatttca ggcagtggac gccaccggca ttttccgcca
     9181 cattgccgcc aattgaacag gcgatttgtg aggaaggatc cggcgcgtaa tagagattat
     9241 gcggcgcaac ggcctaggag atcgccaggt tacatacgcc cggctgcacg cgcgcgcggc
     9301 gaccaacggg gttaatgtcg aggctctctt taaagcgcgc catcaccaac aacacacctt
     9361 tttccagcgg cagcgcgcca ccagaaagcc cggtgcctgc accacgggtc accaccggta
     9421 cacgcaggcg atggcagaca gccagaatcg ctgtcacctg ttccatttgc ttaggcagaa
     9481 caaccagtaa tggacgcgtg cgatacgcgc tcaacccgtc acactcgtaa ggaatgatct
     9541 cctcatcggt atgcaggatc tcaagtccag ggacatgctc acgcagtgcc atcagtaccg
     9601 atgtgcggtc gacatcgggt aaagcgccat caagacgctc ttcgtacaag atgctcatga
     9661 gtaggcttcg ctttgttgtg ttgtgtggca gctgattttt gcgcgctgct tctgtgaaca
     9721 gttattaagc gggcttttcg ttttcgtcta tctctttagc taccggtcag accatttttt
     9781 ttccagctct gtgaccttgt cttggttaac ttaatgttaa attgatgtaa cataatcact
     9841 tgcgtgatgt gcgtgttttg cgagttaaga acagaaaaat tggtcctacc tgtgcacgag
     9901 gtccgggaat gaaagatgaa cgtcgcccta tttgcgaagt ggttgcagag agtatcgaac
     9961 ggttaattat cgacggcgta ctgaaggtcg gtcagccgct tccctcggaa cgtcgactgt
    10021 gtgaaaagct cggcttctca cgctccgcac tgcgtgaagg gctgaccgtg ctgcgcgggc
    10081 gcgggattat tgaaacggcg cagggtcgcg attctcatgt cgcacggctt aatcgggagc
    10141 aggacaccag cccgctgatc catctgttca gtacgcagcc gcgaacgctg tacgatctgc
    10201 tcgacgttcg cgcattactg gagggcgaat cggcaaggct ggcggcaacg ctgggaacgc
    10261 aggctgattt tgttgtgata acccgctgtt atgaaaaaat gctcgccgcc agtgagaacc
    10321 acaaagagat ttcgctgatc gaacatgcgc agttggatca cgctttccat ctcgccattt
    10381 gtcaggcttc tcacaatcag gtgctggtgt ttacgctgca atcattgacc gatctgatgt
    10441 ttaattcagt gtttgccagc gtaaataatc tctaccatcg accacagcaa aaaaagcaga
    10501 tcgatcgcca gcatgcgcgg atctacaacg cggtgttgca gcggctgccg cacgtcgctc
    10561 agcgcgcagc gcgcgatcat gtacggaccg tgaaaaagaa tctccacgat atcgagctgg
    10621 aaggccacca tttgattcgc tcggcggtgc cgctggagat gaacaaggtg ggaatgtgag
    10681 cagagtttta ccctatcccc aaggcgaggg gaccgcccct gcacttaaca ggatctggtg
    10741 ttaaatctgt tcatctatac cctctcccga atgaccgccc ctgcgctaaa caggatctgg
    10801 tattaaattt gcttttcata caatcggtaa cgcttgtacg gctccgcccc aatgcgttcc
    10861 agcatgttat tcatgcctgt attggtttcg aggatccatg acatctccag cgcatcgatc
    10921 ttccggcggg caaacggatc gcgtaaggct tcaatcaata acagcgcaat caccgggccg
    10981 atgcggctga actgatactc gtcgcgcacg cccatcagcg gtactcgcgc agttcgcaca
    11041 ccgctgactt tcaaacgcca cagcaatttt gcccagccga agggaaagag cgatccgttc
    11101 agatcggcaa tcgcctcgtt gatgttaggc aagccacaat aaacgcgcag ggtgcagaat
    11161 caatctcagc gatgtagatc atatcgtccg gcaccagata tttaagttgg tcgcccatgg
    11221 tcgcgaattc atgttcggta aacggcacaa atccccagtt gtgctgccag ccagagttga
    11281 aaatctcacg caggatctgc atctcttcgg caaaccgctg acgattgatg cagcgaatgg
    11341 tcaccttttt gcgcacctga tccatcagtt tttttagcgc cggagagaaa gtgagatcgg
    11401 ttcgctgcat ccaccacgcc agtaaatcaa tgcctttgtg ataacccagt tgttcaatat
    11461 gcgcggcata ccacggtttg ccgtgtggca tcatcgcaca gggtggtgtg tcaaaacctt
    11521 caatcagtaa tccgctttcc tgattgatat tcaggctgaa aggcccgctg atcttacttg
    11581 caccttgtga cttcaaccac gcttccgctg cgccaaacaa cgcggcaaaa tcctgcggat
    11641 catcaatggc gtcaatcatg ccgaaatgac cggtatcttc accgtaacgc tcacggtgca
    11701 aggtatctat ttgcgcggta atacgcccaa ctatctgccc cgcttttttt gccacccacg
    11761 cctgccagat gatatggtcc gtccccggat ttttcgcaga caaatgctcg ttgcgttcaa
    11821 tgaataaagg gggtatccag tttggatcgt cgggataaag tgatgacgga aaagcgataa
    11881 atgccttaag gtcattttta ttaattactt tttcaatttt aatcatcagc agatcgcatt
    11941 ccatatctct gtggataaca aataattaat ggtgaaaaac aggttgcaac agcaacgagc
    12001 ggcatcttaa taatcaactc aatgaataca aacgacgaat aaatagtatt aattaatgat
    12061 tatgataagg tcagaggcta cacagaaacg cctcaatgcc ttttgtcaat ttaaggcgac
    12121 cggacaaggc ttcttcttaa tatattcata aattaaatat cattcataat aaaaacaaaa
    12181 aacttaaact cattatttaa atcaatgttc attttcacac cgatgaaaat actatctcat
    12241 ttatctttta tgcctatata atccaccgca ttattgtcat tttatttttg atatccaatt
    12301 ttttcgtggt gtatatgtct aataaaatct ttacgcattc cctacctatg cgctatgccg
    12361 attttccaac gctggttgat gctttggact acgccgctct gagtagagcc ggaatgaatt
    12421 tttatgacag acgttgccaa cttgaagatc aactggaata tcagacatta aaaacgcgtg
    12481 ccgaagctgg tgcgaagcgg ttgttatcgc tgaacctgaa aaaaggcgaa cgcgtggcgc
    12541 tgattgccga aacgagtagc gggtttgtag aggctttttt tgcctgtcag tatgctggct
    12601 tagtcgccgt cccgttggcg attccaatgg gcgtcggtca gcgggattcc tggagcgcca
    12661 aactccaggg tttactggca agttgccagc ccgcagccat tatcactggt gatgagtggt
    12721 tgccactggt caatgccgcg acgcataaca accccgaatt acatgtttta agccacgctt
    12781 ggtttaaggc attaccggaa gccgatgttg cgctccagcg tccagttccg aacgatatcg
    12841 cctacctcca gtacacctcc ggcagcaccc gttttccccg tggcgtcatt atcacccatc
    12901 gcgaagtgat ggctaatcta cgtgctataa gccacgatgg cattaaatta cgccctagcg
    12961 accgctgcgt ctcctggcag cctttctacc atgatatggg actggtcggc tttctcctgc
    13021 cccccgtcgc cacgcaactt tcagtagatt atttgcgcac tcaggatttt gccatgcgtc
    13081 ctttgcaatg gcttaaattg atcagtaaaa atcgcggcac cgtttccgtt gcgccgccgt
    13141 ttggctatga attgtgccaa cgccgcgtga atgaaaaaga tctcgctgaa ctggatcttt
    13201 cctgctggcg cgtcgctggt attggtgctg agccgatctc cgcagaacaa ctccatcaat
    13261 tcgctgaatg tttccgtcag gttaactttg acaataaaac tttcatgccg tgctacggac
    13321 tggcagaaaa tgcgctggct gtcagcttct ctgatgaagc ctccggggtt gtggttaacg
    13381 aagtggatcg cgacatcctc gaatatcagg gtaaagccgt cgcgccgggt gcagagacac
    13441 gcgccgtatc gactttcgtc aactgcggca aagcgttgcc ggaacatggt attgaaatcc
    13501 gcaatgaagc aggtatgccg gtcgcggaac gtgtggtagg ccatatttgc atctccggtc
    13561 ccagtctgat gagcggttac tttggcgacc aggtttcgca agacgagatt gccgcgacgg
    13621 gctggttaga caccggcgac ctcggttatc tgctggacgg ttatctgtat gtcaccggac
    13681 gcattaaaga tctgattatt attcgtggcc gtaatatctg gccgcaggat attgaatata
    13741 tagcggaaca ggaaccggaa attcattctg gcgatgcgat tgcttttgtt accgcccagg
    13801 aaaaaatcat tttgcagatc cagtgtcgga tcagcgacga agaacgtcgc gggcagctta
    13861 tccacgcgct ggcggcacgg atccaaagcg aatttggcgt gaccgcggct atcgatctgt
    13921 tgccgcccca cagtattccc cgaacgtcct ccggcaagcc tgcccgtgcg gaagcgaaaa
    13981 aacgttatca gaaggcttat gctgccagtc ttaatgtgca ggaatccctg gcataaatca
    14041 aactgtcgcg gtgacgggcg ctaccgggtt tatcggtaaa tatattatcg ataacctgct
    14101 cgcccgcggc tttcacgttc gcgcattgac gcgtactgcc cgcgctcacg tcaatgataa
    14161 tctcacctgg gtgcgcggtt cgctggaaga tactcattca cttagcgagc tggttgccgg
    14221 agccagcgta gtggtccatt gcgccgggca agtgcgcggg cacaaagaag agattttcac
    14281 ccgctgtaac gttgacggca gcctgcgcct gatgcaagca gcaaaagaga gcggcttttg
    14341 ccaacgtttt ctgtttatct cttcgctggc ggcgcgccat cccgagctct cctggtatgc
    14401 aaaatccaaa tacgtcgccg agcaacgact ggcagcaatg gcggacgaaa ttacgctggg
    14461 cgtttttcgc ccgacagccg tgtatggtcc cggcgataaa gagttaaaac cgctgtttga
    14521 ctggatgcta cgcggcctgc tgccacgact tggtgcgcca gatacacaac tctcttttct
    14581 tcacgtcacc gattttgcgc aagcagtggg tcagtggtta agcgccgaaa ctgtacagac
    14641 gcaaacctat gaattatgcg atggcgtcgc cggtggctat gactggcaac gcgtacgaca
    14701 gcttgccgcc aaagcccgtt gtggttccgt gcgaatggtt gacattcctc tgccggtact
    14761 cacaggcctt gcggatatca gtaccgcgtt gagtcgcctg gcaggtaaag aacctatgct
    14821 gacccgctcg aaaattcgtg aattaaccca cgccgactgg tcggcaagta ataaccgtat
    14881 ttctgaagat atcaattggt ttcccgggat tagcctggaa cacgcattac gcaacgggct
    14941 attttgattg tcttgtttta aaggtgtgtt aacgtttcta tggtaaatcg tgaaatagta
    15001 atggattaca tcctttcctg tttacaggat ttggtcgaaa acggtgtgga aattaagccc
    15061 gacagcgatc tcgtcaacga tcttggcctg gagtcgatca aagtgatgga tctcttaatg
    15121 atgctggaag acagattcga tatttccatt cctattaata ttctgctgga tgtcaaaacc
    15181 cctgcgcaat taatggaaac tctacttcca tggctggaga ataaataatg gggctatacg
    15241 ataaatatgc gcgccttgct ggcgagcgtc tgcaatttag tgacaacggg ttaacaccgt
    15301 ttggcacctg tattgatgaa gtgtattccg ccaccgaagg ccggatcggc aataaaaaag
    15361 tgattctggc cgggaccaat aattatctcg gtttaacctt taatcatgat gccatcagcg
    15421 aaggtcaggc cgcgttagcg gcccaaggca ccggcaccac cggttctcgt atggcaaacg
    15481 gcagttatgc gccgcacctg gcgttagaga aagagattgc cgagtttttc aatctcccta
    15541 ccgctatcgt ttttcaaccg gttataccgc taatttaggc gtcatcagcg cacttgccga
    15601 ccataacgcc gtggtgttac tggatgccga cagccacgcc agtatttatg acgcctgttc
    15661 tctcggtggc gcggaaatta ttcgtttccg ccataacgat gctaaagatc tggaacggcg
    15721 catggttcgc ctcggtgaac gggcgaaaga ggcgattatc atcgttgagg gcatctacag
    15781 catgctcggt gacgttgcgc cgctggcgga aattgttgat atcaaacgtc gtctcggtgg
    15841 ttatttgatt gtcgatgaag cgcattcgtt tggcgtgtta ggcgcaacgg ggcgcggact
    15901 ggctgaagct gtcggcgttg aagatgatgt ggatattatc gttggtactt tcagtaaaag
    15961 tctggcctcg attggcggtt ttgctgtcgg ttcggaagcg atggaagtgc tgcgctacgg
    16021 cagccgcccg tatattttta ccgcctctcc ttctccgtcc tgcattgcga ccgtgcgctc
    16081 gtcgttaaga actattgcca cacaaccaga gctgcggcaa aagctgatgg ataacgccaa
    16141 ccatctgtat gacggtctgc aaaaactggg ttatgagtta agttcacata ttagcccggt
    16201 agtgccagta ataatcggtt cgaaagagga aggtttacgc atctggcgcg agcttatctc
    16261 gccgggcgtg tatgtgaatc ttatcctgcc gcctgccgcg cctgcaggca tcacgctgct
    16321 gcgctgtagc gtaaatgctg cccacagtcg tgagcaaatc gatgccatca ttcaggcatt
    16381 tgctactctg aagcagtaat gtgagcccgc cgtggtgttt gcgccacggc gggtttaatg
    16441 ttgtctgtca taccaataca ccaggctcaa cgccaccaac acaaacgcta ccggtaaacc
    16501 gagcgcggta acaggtgcgc ttaacgcaaa tagcaatccc agattaacca tgatttgata
    16561 gctgatccag gtgagtaagc ccacgatgac gcctacagcg agacggctac ccatcccagg
    16621 cgaacgcggg gcactaaagg taaacggcac agccagcaaa atcatcgcca gggtcaggat
    16681 gggttgcccc agtttttccc acagtgcaag gcggtattcg ctgctgggtt gcccggtttc
    16741 ctgcaaataa tggatgtaat gattaagctg acggacagaa aaagtattcc ccggcattga
    16801 taactcttca agattcatac tggtgaaggc cgattgccag gcaagattat ctaatgtttc
    16861 cagcgtttct ttgccattca gccatttttt atgattcaca ccatgcagga cccacgtttt
    16921 gtcgtcttca atggtggcac tgcgtgcata aatgtaggat tcaagcgaga gatcgtcgcg
    16981 ataatgaaat atctccacgc ccaccggctg gccctgctcg ttcagcgatt tcaccgtcac
    17041 aaattcatta ccacgcctgg cccacagcat attgccggta atgtcatcgt cctcccccaa
    17101 cgcggtggcg gtcgatttga tttgcaacgc ctgttgctgc aatggcgacg ccacccactc
    17161 atctatcgcg cctaacgaaa cagtccacaa tatcccggca accagcgcca ccagtgcaat
    17221 acggaagata gaaaaccccg tgctgcgaat ggcggtaagc tcactgtttt tcgataactg
    17281 ccccagtcca acaataccac ccaataacgc gataaacggc gaaagttcga ccagtgtgcg
    17341 cggtaaggtc attagcacca ccagcaccgc ctgagtccag cgataaccgc ccgggctgac
    17401 accatccagt tcgttaatca ggttgaaggt ggtaaaaagc ggcagcaata gccctgcagc
    17461 ggcggcaaaa ccgagaaaga gatgacggat taaatagcga ctgaaaacat tcatcgggaa
    17521 aatttctgca gcaaagagaa gtcacgtgcg accagtatca gcaggcccat tagcatgagt
    17581 ccgggcacta accacaaacc aggaatgagg ggaatcgcgc cgttagccac cagtgtacgg
    17641 cagacgtcgc cgccataaaa aatggcaata aacagcaacg tcagtggcaa taacgttgaa
    17701 aatcgccctt gccgcggttt tacccgactt aatgaaatgg ccagcagcgc catcaacaat
    17761 gcggtcaggc cacggctttg tcgccattgc agttcggcat gatcggcagg aaaggcggag
    17821 cgcgccagct ccgtgaccga tttcgctttg cgtttgacgt tcgggctttg atccagcgga
    17881 ttcagatgta attgcagatt acggtagatc tgttcgttgt cgtcacgccc ctgatgatcg
    17941 agaagatagg cagtgccgtt gtgcaacata acggtcggtt tctcgggtga tgggtcaacc
    18001 acatcaaccg aaccggcgcg gaaaatacgg gttcgattgg cagtagaagt ataaatcagc
    18061 gcgtcagtca ggcgattatt atcctgatca accgtctgcg aaaggatcat tcgtccgtta
    18121 tcgttagtgt taaatttctt tgcccgcaac tggcgaacat ccagctccga ctgcgactgc
    18181 tgctccagtt gataaatctg cgcataagcc cacggacgac catacatcga cagaagggtg
    18241 acaaaaatac ttagcggtat cgccagataa agaacggctt tgtacaagcg tcccggactg
    18301 ccccccgccg cagagatagc ggtaatttcc gagtcggtat acatctgccc tagcgtcacg
    18361 ccaactgaca catacagacc aacaggtaac aacatctcca gtgcaatcag tactttgtag
    18421 aaaacgatat ccagcacaac atcaagagct aatgtcccat ttgccgcttc ggtcagataa
    18481 cgctgtgcgg agtagctggc gaaaataaag atcagaaaac cgacgataat cagcaccaga
    18541 cggcgcgtac cacgcatgat gtagtgttca accagcttca tctcactaac gccctcaatc
    18601 ctttgagttt tttattcatt gatcagtttc tgcgccaacg caatgacatg atctacctct
    18661 tccggcgtca ggctgccgtc ctttgcccac tgaatttgcc cggctttatt cagcaccaga
    18721 atggttgaac tctgctccgg caagcgccag gcaacgcgtc cctgtccatt gccgtcaata
    18781 acgaattgcg cccacggata gcggcgttta tttttttcga ttttgccaac aacaaaatag
    18841 ccagtaccaa agatggcatc atcggtgttc acaatggtgg tgggctgaaa acgatcctgc
    18901 ggaaagtttg ccgcttcaac cgctttaatt aacagtgagt tctttttctt cgccgactta
    18961 cgcccggcga tatactgaat aatgcggact tttcccgcta attgtgtgct gttccacttt
    19021 tggtagtgaa tttcgtcgtc gctattcaga agcagctcgc cgccttcctg gatataaaca
    19081 ggggtaaccg ttttgccagt gacgaaattg tgtgcctgca cacccggggc taagagcatg
    19141 ataatcaacg caataatcaa acgagatgac atcattgctc cggctatagc ggaaaacgcg
    19201 aaatcagcag ctctaagcgc tgacaaatat cgctatccag cgccaacggt gcataatacg
    19261 actggcggtt ttccatatca tccgccacca tgtcgtcgtg tttatctccc atcaggccat
    19321 taatggcggc gaagaaacgg gaaatctgta acgcctcttt attttcagga tcaagcgatc
    19381 ttaaggtgtc gtaataagaa acctgctggc tgatgatttt tcgcacatca tgaaattccc
    19441 tgccggttag tcgcggtttg gcaataatct ctttcagggt atgcatttgc ttttgccgat
    19501 aggcaataaa actgcttacg ctttctaact ggctatggcg taatgaatag ttcaccaacg
    19561 accagaccgg agagcttaaa taaatccgta acaggttgcc gtaatacgac acaatatttt
    19621 tcttaccgtt acgaaagccg tcctgaaaac gccccagaaa aacggtcgtt ttgccgaata
    19681 aaaatccggc ctgatgcttc tttaaataca gccgttgcgc aaaacgcagg tgcttgtagc
    19741 gcgctcgcat gtatttaaat tgtttgcgct catcagcagt cagctcatcg cctttggctt
    19801 gtttgcgaac taaacgcagt aattcctgac gatcaacgcc attgagccag aattgcaggc
    19861 ataacgcgta attatcatga atgatttcgg tggtacaact tagtgcaata gtcggcggca
    19921 aactaacctg tgcatcaacg acatcatctt cttcaatcgc aacaaataga cgctcgctcg
    19981 cctcaagcgg gaaatatttt ctcatcatca ggatttctct ccggctgcgt tataaagcaa
    20041 ggaatggcgc tgaaaaaatg cctttaaggt tggaattaac gagagcgcca cgtccagaca
    20101 ttgcccaccg aagtaagcaa acgctgctcc cagcacacca aacagatgac agaacatgaa
    20161 cagcaatacg atgtagccga ttgaggcgat ggtttgcgcc acgagaaaag cgcgctgttt
    20221 tcccgccatc aatagcaatg actcctgcgg gaagcccagc atcgaaatca caattgcgcc
    20281 cagcatcacc tgaatcaggt catacgcttc gagatactta acgccaaaca ccagcgaaat
    20341 gagcggcttg ccgacgatca acaccgcgag agccaccaga ataccgattc cgcccgccag
    20401 taaaccggac ttcacaccta acaaccacgg tctggtggtg cgcggatcta aacgcatcac
    20461 ctctgggtaa aaacttttac ccagcaaacc cgctggcgtt ccggcggcgt cgaagaatgt
    20521 catggcgatt ttaaataacc cggcggcagc gggtcctaac acgatcccca ccaacactgt
    20581 gctgcacgag ttacgcgccg accagatgga gtgggcaatg ttggttgacc agacaaaact
    20641 ccacgcgcct ttaatgtgtc gggcagactc aaacagattc aatttgaagg cgttatggat
    20701 atttctacgg cgtaattcgc gcgcggcaaa ccaccagtac atggtgccgc caaccagatt
    20761 cgacacgtac caggcaataa caaaacccgc aaaaccaaag tcaaaatacc aggctgcgac
    20821 gctccccgct gcgcgcagaa aaggtttcgt cgcctgctgt acagcaatca aatcaaagcg
    20881 atctaccgca cgcagaatgc cggtcggcgt ggaggaagcc attgaaggaa tgagcgtgca
    20941 atacagcgcc gccagccaaa agctttggtc atccagacct aatgaatggg aaaggaatgg
    21001 cagtaaggca atgccgccga caatcgccac cgcgccgctg acgatatcca gcgagaagga
    21061 aaatgagacg acattgcgaa attgctgcgg attattgttg gtcaaggctg gcgttccgta
    21121 ctgaaccacc agctgccatg tctgaaactt aataaaatcg ctgatcgact tggcgtacga
    21181 ttgcacaatc accagtacac caaacatggc gggcgtcatc cctttaccgg cacacgagag
    21241 cgccaacagc cccagcaagg cgctctcgac attactggag cctaaccagg cgctattgcg
    21301 aataatggtg cgaaacgcgc catctgcaaa ccaatgtttg atgttaaaac ccgccaattc
    21361 agcctgacct tatcgttgat agtaaaaaat atcccgccag ccttaagtta aacttcggcg
    21421 gtcagaaacg atggcaacca gagaaaccgc cttctgtacc tgttccagca cttcgctgta
    21481 gggcgctctg gaatcaatct caagaatttt tgtgccgtta tagccaatcg tcgacatgac
    21541 gccgattttg tcctgcagct cggcatactc atggtcaggc ttgcgggaga tggcagtatc
    21601 aatatcaatc cccaaacgaa tgattaattc aggacggtat tgcgccattt tttggtataa
    21661 actccgttcg cgctgcgcca gaaacatgct gattttcccg gtcgcacgtt cgacgccgat
    21721 ccccggtccg tcataataaa agcccgaaat ttcagcctgc gggaagcgat cgctgaccac
    21781 cagaacgcca ctttgcgcca gtcgctgaac cttgcgtaga ttcgccattc ttcgcagcga
    21841 gaagcagtac ataatcaccg ccgcccatag cgccggagat ttggttttca tgctttgggt
    21901 tttcgaggat ttggccgcca atcgccgttc cagccagacg ccaaccaacg gcaatcgttt
    21961 gattttgtcg ccgtcttcgc cggagagcag ccccagatag cggcgctcgg tttgccagtg
    22021 ttgttgcagc gatttcacca ggtcggtggt cagcgtggat ttaccggtgc catcacaccc
    22081 gaccaccgca atcagccccg gaatgtagtt gggctgcggc gcggttgtac tattaacagt
    22141 ttgtgtttgt agtgcatcca ttcgttaaat catcccctat ccattttggt gatcaggaaa
    22201 gcgcggcgtg aattgcgcgt agtgacgctt gcataatttc atcggctggt tgccgaccat
    22261 ccagttcaag gatcttggcg ccattgaatg tcagttgcgg cgtaacggcg attttttcct
    22321 gtaacgctgc cagttggtgg tcaggtttac gcgcaaacgc ggtttgttca tcaatgccaa
    22381 gacgaatcaa caatacgggt aaataagacg ccatccattg gtacagcttc aactcgcgct
    22441 gccttaacat ttttatccaa ccgttaccgc ccgtggtttt tgccaattgc gggccatcaa
    22501 agcgaaaacc cggcacttca acctgcgggt agcggtcggt gatgagcaga tagccttgct
    22561 ggcttttaca caacattttg cgaaacttgt acgcccgcca gcaggaaagc agatagataa
    22621 ccagcgcagt aatattgcca ggcggagtta agggcttttc gtgtacatgt gccgctttac
    22681 ttcgcagata acacccacaa ggtgcgccaa taacagggag ctgtgaaatc cattcgccaa
    22741 ttcgcccgga cgattgcccg agataaatgt gttctgttgg cattcttgct gccagttcat
    22801 ttaccaggct tgccgtgagg gtcgatttac ctgaaccatc acatcctaca atggcaataa
    22861 cacgcactgg cgtggaatta attattgaca tattacgttg attcacgaaa aacccggcag
    22921 taaataatgt attgaatatt acgtggtccg tgctcagaat atccgttcaa ccttattgtg
    22981 acaatggcta attgtgacat tgacctgaat tacgctttca taaaaacata ttaaccaaat
    23041 aaatattttt aatggatatt taaattaaag gatatattca tgcagtcaat aacacctcca
    23101 ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga acatcttatt
    23161 accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca ggccggaaat
    23221 gtcggtcgtg cagtgacaaa attacctttg atgggaaaat ccttacataa aacaattgaa
    23281 cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct ggtaattaca
    23341 gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg tcgtcgcggg
    23401 ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta cgatggtacg
    23461 gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag ccaggaacgt
    23521 aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact caatgttgac
    23581 cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc gaggaagatt
    23641 gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga tgccaatcag
    23701 ccactggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat gaccgcgcgt
    23761 ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt ttcaggaagg
    23821 ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg ccgccggtaa
    23881 agtaaacacc cacgccatca ggatgctggt taccgtttta cgctgtaacc caccgccgtc
    23941 caccaccatc gtccctgcta ccgcagacga gaggacgtgt gttgtggaga caggcatccc
    24001 aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca tgccttgcgc
    24061 atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac gacgccagcc
    24121 aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca ccggagcgta
    24181 ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt cttctttact
    24241 gacgcctggc agtttcgcca gcttcgcgga ggtatcggag atgcacagca taatgcggcg
    24301 cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc ctggcagcat
    24361 cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa actctgttac
    24421 ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atcgcaatca acttctgcgg
    24481 cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc gggtacgggt
    24541 aatttcatag ccggacgcat tcatattgac gacgaagcca gcaggggcaa tccccaccag
    24601 taccagcatt accaggccga tccctttctg cccgtcgttc gcgccgtgcg aaaacgccac
    24661 gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac gtttgccctt
    24721 tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg tcccactcca
    24781 gaaacggcgc agcaggaaaa tcagaccacc cgcaatgacc aggccgacga taggggaaac
    24841 aatcagcgag gagaaaattt tggttacttc acgcaggttt aacgcatcca tcaccgatga
    24901 gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca aggtgtgcga
    24961 actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg ccgccagcag
    25021 catggaaaag accatcgcca aaccgtgagt tgaccccata ttcagcaaca aatcgattgg
    25081 taacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc caaaaaagtt
    25141 aaaaaatgcc gccatcacca cagcaagttg tggctgcatg gcacgagtat aaataacggt
    25201 tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca acacaaatgc
    25261 cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata aatttagcat
    25321 atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac tttaacggca
    25381 tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt gtgtatcaga
    25441 caataagcat tcattcaaaa cctttagaaa taatcaaaac acaggtattc cactggttaa
    25501 tatattcaca tatgaaatga ataaatattg gaatatataa atattgaata ttttgattaa
    25561 tccccgtact aattattctt cataatcagt acggggttgc tacaacatgt attacttttt
    25621 caccacaatt aacggttcaa tatcactctc ttttttgatg accagtgatt catcaccgcg
    25681 caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa tgtatttacc
    25741 gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg caaatttacc
    25801 gctggttttg tccagcacct cttcatgatg gctgaccagg tcgatattgc taccacagcg
    25861 accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc
    25921 ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt
    25981 gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc gcagcaatac
    26041 gtcgataaga cgcacttcgt tttgcggatg accggtacgg attggcaccg cagcaaactc
    26101 acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg ttttccacac
    26161 gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc agcccagttc
    26221 atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa
    26281 ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa acggacgtgc
    26341 acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg gattgaagcc
    26401 gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca
    26461 ggaggcggag tcggcgttgt attcgtaaac cttcaggcca cgctcatcca tgcagaaatc
    26521 catacgacca gtgatcatat ggtgacggcg acgctgccag gagagacgca aacgtggcca
    26581 gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca ccttgtcggt
    26641 tgcgtgaaga tacatcagat gcagctcgtt ggtggcttta attagctcct gctcggcact
    26701 ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt tggcctgcac
    26761 ataggcgttt tgtagcggat ctttttcatc cagccattta ccgtcaaact ggcctttgtt
    26821 ttccagacgc gctccactga ttttcagcag ctcgcctgca atttccggct gcggtaagct
    26881 gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc
    26941 tttaagggta tagcagccgt tttcgaccac catctccagc tcgcgcgtcc actgttgccc
    27001 ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt tgccatgcaa
    27061 ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat cccagataag
    27121 aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca atggcaggat
    27181 gttgtcatta accacttctc gcaggaagcg cagcgagaaa atctcccacg ccatacccac
    27241 gtcagtaaag accacaccgt aattcagaaa gagaaaacgg cgagcaaatt caacgcattg
    27301 ccacttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt catcttcgta
    27361 ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac ctggggcgta
    27421 gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca tcactttacc
    27481 tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc tgctcagcga
    27541 cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg gcgcaaaggg
    27601 ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac actaccactc
    27661 agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg gacgtgggat
    27721 aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgt tttctggtcc gacgcatgaa
    27781 aaaacgctgc ccgttggcaa acatccattg caactttatt cgctgggaac gccgaacggt
    27841 cagaaagtaa cgattatgct tgaggagcta ctggcgctgg gcgttactgg tgcagagtac
    27901 gacgcctggc tgattcgtat tggcgatggc gatcaattct ccagcggctt tgtcgaagtg
    27961 aacccaaact cgaagatccc ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg
    28021 tttgaatctg gttcgatcct gctttatctg gcggagaaat ttggctactt cctgccgcag
    28081 gatctggcaa aacggactga aacgttgaac tggctgttct ggttacaggg tgccgcgccg
    28141 ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat tgagtacgcc
    28201 atcaaccgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa gcaactggcg
    28261 cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat ttggccgtgg
    28321 tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga tgcgggaagt
    28381 tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt gaaacgtggg
    28441 cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg ccatgacgcc
    28501 agtgattttg agacaaatac agaagataag cgtcaggggt aagggttggt gttcgtcgca
    28561 gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct
    28621 gatgcgacgc tgccgcgtct tatcaggcct acaaaagcta atcatcacgc gctggtaatg
    28681 tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg
    28741 ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc agggttacct
    28801 tcgcaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc
    28861 tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca cattgttaac
    28921 tccggttatt tcgattgccg gacaaacgcg aaaaagaacc tttagcgggt gttccctctc
    28981 accctaaccc tctccccgga ggggcgaggg gaccgtccgt gcgcttttta ggcgttgtcc
    29041 tcagtcttat tcaacttcaa tacttttgac gatcagcgaa tcgccggtat cgacacgcaa
    29101 ccgctcgccg tgacagagcg gacactgcgc atcgtgctga tgaatctcca ccacctggct
    29161 gcaatcccag caccaggcct gggcgggttt atagacgata tgtaaatcgc acccttgcgc
    29221 caccgttccg tggcagacaa tttcaaaact aaaacggacg gcgctctcct caacgcagga
    29281 gagcgcgcca atttccagcc acacggcggt gacgcgctta acatcgtgct gctccgcctg
    29341 ccgttggata atttcaacgg cgctctggca aagcgacaac tcatgcattt tcgctcctcc
    29401 tgcggccaaa cagcagggcg cgacgccctg cgtgtggtac atccggattg gtgactggca
    29461 acgaaaggat cattcgtgcg cagtcatccg tcaggcgttg gccttcttca atcgacatgc
    29521 tgtgcgaaag cggcgacatc agcgaacagg agaggtattg cgaaacaccg tccagttcgc
    29581 caacggtaaa ggtcatggtg ccatacggca gttgcagacc aattttttca ctcactttgc
    29641 gcagcggcca gagttgatcc gggccgggga agataactgc actcagcatc cacggggtga
    29701 tcacacaccc cgtccactga ccttcgaaca gcgtaaaatc agaaacatac accggcattg
    29761 aaggatgcag aaaagagaga tcgtgcatcg aacgccgggc aatttcttca aacgctgcct
    29821 gtacttgcgc cttcggggag gtctggaaac ctgctatctc ttcagtcatg aatcgcctcc
    29881 cgtgggatgg cttccacgcc tgattcgcgc agcgcagcca gaacctgctc aagcgcaggt
    29941 tcaatcattg cttcaaccgt cggcgttaag ccgatgtgtg gctccagcga ttccgggatc
    30001 acgccgacca gggtcagctt tttcggaaac tcaccggtga agcgcagggc cgacaatacg
    30061 tcggccaggc caagctgatg cggagagatt ttgttggtaa acaacgccgg aacttcttca
    30121 tcccgcagga tcatcatcgt tcccggagtg ttctttttcg acacaatggc atccgcaata
    30181 atcagatgat cgcgatttgc catgtcgcca agcagctcca ttcccgccgt gccgccatcg
    30241 aggatctcaa cataatccgg cagaatgtat cgttgctcta acgcttcgac aatccgcacg
    30301 ccgatggctt catcggtcag caaaatattg ccgaccccta agactaaaat acgcattaca
    30361 gaaccttcac tgaaaccact tcgttgccgt cggcatccac tacgtgtacc gcacaggcca
    30421 tgcacgggtc gaaggagtga atggtacgca ccacttccag cggtttattc ggatcggcaa
    30481 ccggtgtacc caccagcgac tgctcgtaag gaccgacgtc atcattgaag ttacgcggac
    30541 cagagttcca ggttgatgga acaaccgcct ggtagttgct gatgataccg tctttaataa
    30601 ccatccagtg agagagcata ccgcgcggcg cttcgaggaa gccaacacct ttgaactcac
    30661 ccgttgccgg aatgttcggc ttcacaaagg tggtgtgatc gcctttgccg atattggtga
    30721 tcagtgcact gtattggttt tgcaggatat cctgcaattc acagcagtga acggtacgac
    30781 caataatacg gcccagcgtg gagtgcagct gtgccacttc cagcgtgttg ccagtcagtt
    30841 tctgataaat cgcaacgatt tcattcagtt tgttctgggt agattcgcga cctgccgcca
    30901 gtttcaccag catattagcc agcggaccca cttctaccgt tttgccgtag aaagtcggtg
    30961 atttcaccca ggaatatttc ccgtcgtcag accaaccatc ataagccgga atggtggtac
    31021 cttcccacgg cgcctgtggc gcttcgtctt tataccagga gtgcttcgcg ctttcctgaa
    31081 tgcctttgat caggtattca tcggaatgag aagtgatcgg acgatacgag gacagatccg
    31141 cattctcaat gtagcctccc gggaacagga agctgccgtt tttgctgtcg gtcgggaatt
    31201 ccggcacgct caggtagttc accgcacctt taccgcgcgt cagccattcc gggtagaacg
    31261 cggcgataac cgcggtatca accttataaa cctgctcaac aaagtcgctc agtttgtcga
    31321 tgaaagactt gatgtacatc aggcgctcaa ggttcaacac gcccaacccg tcgaggttga
    31381 ttgggttcgc gacaccaccc actgccaggt tctgaatgtg cggcgtttta ccgcccagca
    31441 gcgccacgac gcggttagcg tcacgctggc actccagcgc ctgcaggtag tgcgctaccg
    31501 caatcaggtt cacttccggc ggcagtttca tcgccgggtg accccagtag ccattggcga
    31561 aaatacccaa ctgaccgctg gcaaccagat ctttgatctt gttctgaact ttagtgaact
    31621 cttccggact gttcagatgc caggtcgaaa cgcctttcag catttcggag gctttggttg
    31681 ggtcagcttg cagtgcagaa gtgatgtcca cccagtccag cgccgaaagc tgatagaaat
    31741 gaacgatatg gtcatgcgtg gtgtgcgcag ccagaatgat gttacggatg tattgcgcgt
    31801 taaccggaac gtcgatattc agcgcacttt ctgccgcacg aacggaagac agcgcgtgag
    31861 tggtagtaca tacgccacag atacgttgca caatcatcca tgcatcgcgc ggatcgcggt
    31921 ttttcacgat ctcttccatg ccgcgccaca tggtaccgga agcccatgct ttcgaaacga
    31981 cgccattttc gatttcgcaa tcgatgcgta aatgcccctc aatacgggtt accggatcaa
    32041 tagtaattct ctggctcatg ctttgctcgc ctcatgacga ttatgatcgt tttgttttaa
    32101 aggaggaagt atcggcagta gacgaatgag tacgatgtaa gcgcaaatct caatagccac
    32161 aaaaccaata gaaatcaaca gttcttccca ggtcgggaag taggcgtaac cgccgcccgg
    32221 gttgaatgcc accaacgaat aggtcagacg ccaggttgca caacctaaca gtgcgctcag
    32281 tgctgacagg aacagcatgc gggaatcatt acgcagcttc gccacacgca gaacgaccag
    32341 cgggaagagc atcagcagga cttcaatcca gaacatcacg gagtagaagt caccggcaaa
    32401 cgctaacgac agcttgtcgc gatagataag ctcgccaaag cgcagcccga tgaaaatcgc
    32461 cagcaacaca ctgatggtgt tggtcagctt gacgaacagg ctcttttcat ccggaccgtt
    32521 gccacgcaga cccgcctgca ccagtgaacc ttcaaagatg acaatcgaga agcccatgat
    32581 gaacgccgtc agcagcgaga acagcggcaa catttcatag ctctgccaga gcggatgcac
    32641 cttgtagccc gccgagatca tcagcgaccc cattgaagac tggtgcatgg tcggcagcag
    32701 cgcaccgagc gcgatgatga agaacatcac cttgttcaga cgttgtagcg acaccttcca
    32761 gcccagacgt tcaaacagtg ccggagcaaa ctccagagcc atcacgccga tgtagatggt
    32821 catacagacc gccgtctcga acagtaccga gttcacgttg aagtgacccg gaatgtagaa
    32881 gtacggcagg ttccagtagc gacccacgtc gatagtgatc gacaagccac ccagtgagta
    32941 accaaacaga ctcgccaaca gcgccggacg caccagcgga tggtattgcc cacggttaaa
    33001 gacgtatacc gcccacgcca gcgcccagcc gccacaggca aagccggtgc caatcagcag
    33061 gtcaaacgcg atccacacgc cccacgggaa gccgccgttc aggtcagaga cagagcccag
    33121 accgaacacc agacgcttca caatcaggag catacagatg acgattaacg gtccaaaaat
    33181 catgaccggt ttactgatga ttttgccgcc cagcggttgt ggatcatgac tcatgatcgt
    33241 ctcctccgtc gtgatggtcg tttttggtgt tgcgacgaac cagcacggtt aagcccgcca
    33301 gcacagccag tggtagcatc atgcctttat acagggtgtg ttgaatattt tcggaacgcg
    33361 caccggtaga aagatcgtcc agtttcggca ggtcgagatt ttcataaggc acacccgtca
    33421 gtaccagaac ctgagtaccg ccgccctctt tctcgccgta cagatgcgga taatatttcg
    33481 gcaccgtatg caggtaagtg tcgccagatt tcagcgtctg acgcggatag tggtattcgc
    33541 tgccaggctt cagcgccaga cgttttttcg cctccgccat cagctcttca cgcgtaccga
    33601 aaatcaccgc gcccgccggg cacacttcta cgcagccagg cagaccgcct ttatcgagac
    33661 gttccacacc tttctggttg cacagctcgc acttatgcag cgcaccaaac gggttgttgt
    33721 agtcgtactt cggcacgttg tacggacagg cgaccatgca gtaacggcaa ccggtgcaca
    33781 cgtctttgtc gtaatggaca atgccggttt tcggatcttt tttcagtgca gagaccgggc
    33841 acacagagac acagttcgga tcgacgcagt gcatacactg tttcttaatg tacgcgtagc
    33901 cgttctcctc ctggtctttg ttgacccctg tgccgctggt ccacacctga atgatgttat
    33961 tggtatacgg cgacagtttg tcgttgttcg accaggtctg ttccccttgc gggttacgtt
    34021 cagggaagtt gatatcctga cacttggtga cgcaagcctg gcagcctacg cacaaggtcg
    34081 agtcgtacaa catccccagc gatcccggaa ttggcgggcg gttttcagca gccgcatgac
    34141 tgacagacgg cagcgcgccc gtcagcaatg ccccgcagga ggctgcttta ataaaattac
    34201 gtctgttcac ggttattctc cccgtgagtc agcgttatct ttcttttgct gacgacccag
    34261 ttcacgcacc gccatcacgc tgacaccggc aaccagccca accacaccgc cgagcaaacc
    34321 aatagcgcct gcagagacgt tgccgccctc tttagcgtta acatccggtt tctgtgaacg
    34381 cggagtctga ttttcgacgt tggcaagctg atggatgcct ttatggaagc cgataccttt
    34441 ttcgttacag ccatagcacg ggtgaccaat cgccaccggc cacacaccgc caacatcgca
    34501 gaattgcagc gttgagcagt tgccgtaagt ttctggccct ttacagccga ggtggtacag
    34561 gcaccagcct tcgcggtggc cttcatcacc gaactctttg gcaaaacgac cagcatcgaa
    34621 gtgcgggcga cgttcgcagt gttcgtgaat cagacggcca taggcgaagg tcggacggtt
    34681 tttgtcatcc agtttcggcg gtttgccgta agtgatgatg tgcgcaacgg tcgcgaggaa
    34741 gttgtgcggg ttcggcgggc agcccggaat attgataacg gttttgcctg gcagaacttc
    34801 ttgcaagctg actgcgccag ttgggttaac tccagctgcg gcaacaccgc cccacgcgga
    34861 gcaggaacca atagcgataa tggctgctga gccttccgcc gctttgcgga tgtgatccac
    34921 aatcggctca ccggcaacca tgcaataaat accgttatct tttaatggga tggaaccatc
    34981 caccactaac acatactgcc ctttgtactt ctcgagagcg ttatgtttgt tctcttcgac
    35041 ctgatgaccg aaggcggcgg aaagcacttc gtgatattcc agagagatag tctccagcac
    35101 gaggttttct accgttggat gcgttgcacg aagcagagat tccgtacaac cggtgcactc
    35161 ctgcgcgcca atccagataa ctggcggacg ctgcgggtta gtaaccgatt cggccatctc
    35221 tgcagcggct ttgctactta accccatggt ggcggctaat gctgcacaaa gcttcatgaa
    35281 atcacgacgg ttaatgccgt gagaatggat gagggtgtta tctccagtca tttatagtta
    35341 ttccgttgcg aggacctggc atatattttg cctcaatcgc aaaatcaata atgcgatcga
    35401 tgcgccattt accacacatt tattatggtt atcgatatca tgatactgcg gcggggcgct
    35461 aaaacgaagg gaagaagcat tagtgtaatt aattaaagca agataatacg tatgtttgat
    35521 caattttcga ggcaataacc ggccataaaa aagcgcaacg tagaacagga attattctca
    35581 cgaaagaaat ccattaccat ctctttatcc agtccatagc ggcgcgtcag aatacccgct
    35641 tcccaggcgc taagctggcg atctccggtc ttttcgtaca tccggtgtga gtagcctaat
    35701 acaaaaccgc gtttataatc ggcacagaat tttgtcacat taaccgcact gtcggcatgc
    35761 gttgctttta atcccgccat taagccttta ccaaaatggt tattcatcgt tcacctcatt
    35821 cgttatatat cgccatatat agcgatattt taggcaaaga acgacttttg cgcacgctat
    35881 tttttcatac ttgtttaaga cgtaatcccg taagagataa acctatcagg agagatattt
    35941 atcgaaccat ttcagtgtcc tttgccaggc aagatcggcg gcagatttgt cataacgggg
    36001 cgtggaatca ttatggaatc cgtgattaac ccccggatag atataagcct cataaacctt
    36061 attattgtct ttcaacgccg cctcgtaagg aggccagccc tcgttgattc gggtgtccag
    36121 ttccgcgaag tggagtagta aaggcgcttc aatcttcgcc acatcggcag tgggtgcctg
    36181 acgaccataa aacggcaccg cgcaggccag ttccggatac gcgacagccg ccgcgttcga
    36241 tacgccaccg ccatagcaaa atccggtaat acccacttta cctgtcgctt gcggatagcg
    36301 ttgcataaac tcaattgcgg caaagaaatc attcatcagt ttggttggat caacctgctg
    36361 ttgcagctca cgacctttat catcatttcc cggataacct ccaacggaac ttaatccgtc
    36421 aggtgccagg gcgatatacc ccgccttcgc cactcgccgt gccacatctt cgatatacgg
    36481 attcagtcca cgattctcat gcaccacccc cacggctggc gttttgccgc tcatttttgc
    36541 gggtttcacc agataacccc gtacctcgcc gtgaccattt ggcgaaggat acgtgatgta
    36601 ctcagcaaca atctccgggt cggtaaactc tacctgagtc gccagcgcat aatttggctt
    36661 gagcaaatca aacaacgcta atgccgtcat cccgcccacc gcatacttcg ccgcaaggtt
    36721 gaggaactca cgtttcgaaa ttttcccgtg aacgtaatag tcgtagtaat ccaacaactc
    36781 ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca ttcctcaact ggtgtttttt
    36841 taagcaaagc ataagcgcgt gtttttgccc agtttttcgt cactctgtga gccagactac
    36901 gggatacgcg ctggcgaatc gctaaactag aaacattgtt tcgaaattga acggaggaaa
    36961 ggagaggtca tggtctggtt agcgaatccc gaacgttacg ggcagatgca gtaccgctat
    37021 tgcggaaaaa gtggtttacg cctgcccgca ttatcgctcg gtttatggca caatttcggt
    37081 cacgttaacg cgctggaatc acagcgtgca atcctgcgta aagcgtttga tttaggcatt
    37141 acgcactttg atttagccaa caattacggg ccgcctccag gaagcgcaga agagaacttt
    37201 ggtcgcctgc tgcgcgagga ttttgctgct tatcgcgatg aactgattat ctctaccaag
    37261 gctggctacg atatgtggcc cggcccgtac ggctctggcg gttcacgtaa atacctgctc
    37321 gccagcctcg accaaagcct gaagcgtatg gggcttgagt atgtcgatat cttttactct
    37381 catcgcgtcg atgaaaatac gccgatggaa gaaaccgcct ctgcgctggc tcatgcggta
    37441 caaagcggta aggcgctgta tgtcgggatc tcctcttact cgccagagcg gacgcaaaaa
    37501 atggtcgagt tgctgcacga gtggaaaatt ccgcttttaa ttcatcaacc ttcgtacaat
    37561 ttactgaacc gctgggtgga taaaagcggc ctgctggata ccctgcaaaa taacggcgtg
    37621 ggctgtattg cctttactcc tctggctcag ggattgctga ccggaaaata tctcaacggc
    37681 attccgcaag attcacggat gcatcgtgaa gggaataaag ttcgtggtct gacgccgaaa
    37741 atgcttaccg aagccaacct caacagcctg cgcttattga atgaaatggc acagcagcgt
    37801 ggacaatcaa tggcgcaaat ggcgttaagc tggttgctga aagatgatcg cgtgacgtcg
    37861 gtattgattg gtgccagccg cgcggagcaa ctagaggaga acgtgcaggc gctgaataat
    37921 ctgacattta gcaccgagga gctggcgcag attgatcagc atatcgccga tggcgagctg
    37981 aatctgtggc aggcgtcgtc cgataaatga cctgttaata acgggcgcgc gaaccgcgcc
    38041 cgaatcagat cagtgattat gacgagtcag tcggtcaaga tagcccatca caaatgcaga
    38101 aagcacaaac gtcagatgga taatgacgta ccacatcagt ttgttatccg ggacattttt
    38161 cgcatccata aagacgcgca gtaagtgaat ggaagaaatt gccacaatcg acgctgctac
    38221 tttgtttttc agcgacgttg cgtccatttt ccccagccac ttcagcttct ctttgttctc
    38281 ggagatatcc agctgtgaga cgaaattctc ataaccggaa aacatcacca tcaccagcaa
    38341 accgccaacc agcgtcatat ccaccagcga cagcaacacg aggatcaaat ctgattccgc
    38401 catcgagaag atattcggca gtacgtgaat aatctcctgg aagaacttca gcgccagggc
    38461 aactaacgcc agcgaaaggc caaagtacac gggggcaagc agccagcgag aagcatacat
    38521 tgcattttca agaaaacgtt ccatacattc ctgtcgtgta gttaaaacgg gcaacagtat
    38581 atcgcaattg agtaaacagc acgcaacagc aaatcaacat atacgttaaa cctttgtcgc
    38641 ataattctga ggtttaggga tcttcctggt tcccttatca acgcatcgag agcgtctacc
    38701 cttaaaggac gttgaacctg aagggagaaa aacgatgtct catttaaaag acccgaccac
    38761 gcagtattac actggtgaat atcccaaaca gaaacaaccg acgccaggca tccaggcgaa
    38821 gatgacaccg ataccggatt gcggcgagaa aacctatgtt ggtagcggtc gcctgaaaga
    38881 tcgtaaagca ctggtgacag ggggcgattc cggaataggt cgcgctgccg ccatcgctta
    38941 cgcgcgtgaa ggggctgacg tggcgatcag ttatcttccc gtggaagaag aagacgctca
    39001 ggatgtgaaa aagatcattg aagaatgcgg acgcaaagcc gttctgctgc caggcgattt
    39061 aagcgatgag aaattcgccc gttcgctggt tcacgaagcg cacaaggcgt taggcgggct
    39121 ggatattatg gcgctggtcg ccgggaaaca ggttgccatt ccggatattg cagacctcac
    39181 cagcgaacag tttcaaaaga cctttgccat taacgttttc gcgctgttct ggctaaccca
    39241 ggaagcgatc cccctgctac cgaaaggtgc aagtgtcatc accacttcgt caatccaggc
    39301 ataccagcca agtccgcatt tactggacta tgcggctacg aaggcggcga ttctgaacta
    39361 cagccgtggc ctggcaaaac aggtcgcgga gaaaggtatt cgggtgaata ttgtcgcgcc
    39421 aggccctatc tggacggcac tgcaaatttc cggcggacaa acgcaggata agatcccgca
    39481 gtttggtcag aaaacgccga tgaagcgtgc ggggcaaccg gcggaactgg cccctgtata
    39541 tgtttatctg gcaagtcagg agtcgagcta cgtcaccgca gaagtgcacg gcgtgtgcgg
    39601 cggcgagcat ttaggttaaa aaaatgcccg gttgtgaaaa gcaaccgggc atcattgtga
    39661 attcactctt acctgatgtg gcgaaaacgc cttatccgcc tacaaacgca tgaagattca
    39721 acatgttgca atagattttg taggcctgat aagcgtagcg catcaggcat tgagcaccta
    39781 atgcctgatg cgtcgtgaac gccttatccg gcctacaaac gcacgaagat tcaacatgtt
    39841 gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg caattctact ttacttcgct
    39901 ttggcggttt cttcgccgac cagacctatc ttcaggtaac ccgcctgatg cagcgtatcc
    39961 attaccttca tcaacgtctc gtaatcgacg gttttgtccg ctcggaagaa gatggtggtg
    40021 tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc ggtgaccgga
    40081 tcgttaccga taaacatcga gttgtctgcc ttcaccgaca gataaaccgg tttttccggg
    40141 cgcggctgcg gcgtgctggt agaagcaggc aagttcacct tcacatctac cgtcgctaac
    40201 ggtgccgcca ccataaagat aatcagcaga accaacatca cgtcgataaa cggcgtcacg
    40261 ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat tgccatcgga
    40321 cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat gcgcagcggc gctggcttcc
    40381 agatccaggt cacggctttg cagcaacaat acctgcgctg caacatcacc cagcatcgct
    40441 ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgcagg aatcgctgcc
    40501 acgaggccga ttgccgttgc taacagagct tctgcgatac ccggcgcaac gactgccagg
    40561 ttagtggtct gcgtttgcgc gataccaata aagctgttca tgatgcccca gaccgtacca
    40621 aacagaccaa cgaacggaga aatcgcgccg atggttgcca gatagccgtt accgcggccc
    40681 atttgacgac ccactgcggc gacccgacgc tccagacgga agctggtacg ttctttaata
    40741 ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc attgagcaaa
    40801 tgcaggctta agcttttgct accaaaatca gcggcgatat cgttggcctg gtttaaggaa
    40861 cgtgcttcag ccagcagttg ctgctcgcgc ttaaggcgac gcttctgatt gaagaactct
    40921 acgctcttac tgaagaagat tgcccaggtg actacagagg ccaaaataag cccaatcatc
    40981 acgcacttaa cgacaatatc ggcgtactga tacatacccc agacggaaag gtccgtctgc
    41041 attaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg cgcataataa
    41101 tatcaaaacg acgtcgaatt gatagtcgtt ctcattacta tttgcatact gccgtacctt
    41161 tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc agcacgccat ttgcgaaaat
    41221 tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta gtttagacat
    41281 ccagacgtat aaaaacagga atcccgacat ggcggacaaa aagcttgata ctcaactggt
    41341 gaatgcagga cgcagcaaaa aatacactct tggcgcggta aatagcgtga ttcagcgcgc
    41401 ttcttcgctg gtatttgaga gtatggaagc caaaaagcac gcgacgcgca atcgcgccaa
    41461 tggcgagttg ttctatggac ggcgcggaac gttaacccat ttttccttac aacaagcgat
    41521 gtgtgaactg gaaggtggcg caggctgcgc gctatttccc tgcggggcgg cagcggttgc
    41581 taattcgatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca acaccgccta
    41641 tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa cgacatcgtg
    41701 gtttgatccg ctgattggtg ccgatatcgt taagcatctg cagccaaaca ctaaaatcgt
    41761 gtttctggaa tcgccgggtt ccatcactat ggaagtccac gacgttccgg cgattgttgc
    41821 cgccgtgcgc agtgtggtgc cggatgccat cattatgatc gacaacacct gggcagccgg
    41881 tgtgctgttt aaggcgctgg attttggcat cgatgtttct attcaggccg ccaccaaata
    41941 tctggttggg cattcagatg cgatgattgg cactgccgtg tgcaatgccc gttgctggga
    42001 gcagctacgg gaaaatgcct atctgatggg ccagatggtc gatgccgata ccgcctatat
    42061 aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt caacatcatg aaagcagtct
    42121 gaaagtggcc gaatggctgg cagaacatcc gcaagttgcg cgagttaacc accctgccct
    42181 gcctggcagt aaaggacacg aattctggaa acgagacttt acaggcagca gcgggctatt
    42241 ttcctttgtg cttaagaaaa aactcaatga tgaagagctg gcgaactatc tggataactt
    42301 cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc tggcaaatca
    42361 accagaacat atcgccgcca ttcgcccaca aggcgagatc gattttagcg ggaccttgat
    42421 tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg acgccggttt
    42481 tgcacggatt gtataacatt gccacttttg gacaattttg cagacatttt attgtgaaaa
    42541 gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg attcaggagt acaataggca
    42601 gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa gatatcatcg
    42661 ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc tcatattgtt agcgttgttt
    42721 actttgtcat gtttgccacg ctgtttttag aaaacggcct gctgcccgcc tcatttttgc
    42781 caggcgacag cttgttgata ctggcaggcg cattgattgc ccaaggggtt atggattttc
    42841 tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta agttatattc
    42901 aggggagctg gttagggaat accaaaacgg tgaaaggctg gctggcacag cttcctgcta
    42961 aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg ctgctggctg
    43021 gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt tccggtctgc
    43081 caaaccgccg cttccagttt ttcaactggt taagtggatt gctgtgggtc agcgtggtaa
    43141 ccagttttgg ctatgcctta agtatgattc cgttcgttaa acgccatgaa gatcaggtaa
    43201 tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgtta ggcacgctgt
    43261 ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccgttc ccggctgctg
    43321 taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc ttcccccggc
    43381 gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc atagccgacg
    43441 cgcatcgctg ctgcgctggc cttcatgccg tcatggatga tcatcatccg cgccttatgc
    43501 agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt aaaattatgg
    43561 tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct caggttttcg
    43621 gtgtatttat tctcaattcg tttcagcacg cggctaatca gactgaagtg agtctggcga
    43681 ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta cagaatttcg
    43741 cggatgatct gtttgccgag aatacgcgca tccagtggtc gctccatcac gtcgagtaac
    43801 cgctccgccg cgcataaaat ctcttctgat aacgtggcgg agttaatccc gctggctgcc
    43861 atcgacggct ggaaatgctc atcttcgcca atgtccatca acagttcctg taactgcaaa
    43921 atatcgacat tgagacgcaa ccctgccagc ggcacctctg acgtggcata ggtttcgcac
    43981 tcaaacggca acggcaccgt cagcagcagg tattcattgg catcataacg aaacacgcgt
    44041 tcattgatat aaccgatttt atgcccggaa aagagaatta tgatgccagg ctcgtacatc
    44101 accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg caacagttct
    44161 gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa gcgacaaatc
    44221 tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga ttgtgcgcaa
    44281 ttttgtagca tttctccagc actctggaga aataggcaag acattggcag aaatgagcat
    44341 tgagagccag ggcgctggcg atcacaatga aaaacatcag gcagatcgtt ctctgccctc
    44401 atattggccc agcaaaggga gcaagtaatg aacaacttta atctgcacac cccaacccgc
    44461 attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca cgatgctcgc
    44521 gtattgatta cctacggtgg cggcagcgtg aaaaaaaccg gcgttctcga tcaagttctg
    44581 gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgagccaaa cccggcttat
    44641 gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga aagtgacttt cctgctggcg
    44701 gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc taactatccg
    44761 gaaaatatcg atccgtggca cattctgcaa acgggcggta aagagattaa aagcgccatc
    44821 ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc aggcgcggtg
    44881 atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcacatgt tcagccggta
    44941 tttgccgtgc tcgatccggt ttatacctac accctgccgc cgcgtcaggt ggctaacggc
    45001 gtagtggacg cctttgtaca caccgtggaa cagtatgtta ccaaaccggt tgatgccaaa
    45061 attcaggacc gtttcgcaga aggcattttg ctgacgctaa tcgaagatgg tccgaaagcc
    45121 ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca tgtgggcggc gactcaggcg
    45181 ctgaacggtt tgattggcac tggcgtaccg caggactggg caacgcatat gctgggccac
    45241 gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt cctgcctgca
    45301 ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc tgcaatatgc tgaacgcgtc
    45361 tggaacatca ctgaaggttc cgacgatgag cgtattgacg ccgcgattgc cgcaacccgc
    45421 aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct ggacggcagc
    45481 tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact gggcgaaaat
    45541 catgacatta cgttggatgt cagccgccgt atatacgaag ccgcccgcta agctttttac
    45601 gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca cctcaccgga
    45661 gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc gttattaagc
    45721 tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca agtaatgagg
    45781 aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt gataccgccg
    45841 cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca gtcaacagag
    45901 aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc cgcgaagccc
    45961 tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctactta atgcactggc
    46021 ccgttcccgc tatcgaccat tatgtcgaag catggaaagg catgatcgaa ttgcaaaaag
    46081 agggattaat caaaagcatc ggcgtgtgca acttccagat ccatcacctg caacgcctga
    46141 ttgatgaaac tggcgtgacg cctgtgataa accagatcga acttcatccg ctgatgcaac
    46201 aacgccagct acacgcctgg aacgcgacac acaaaatcca gaccgaatcc tggagcccat
    46261 tagcgcaagg agggaaaggc gttttcgatc agaaagtcat tcgcgatctg gcagataaat
    46321 acggcaaaac cccggcgcag attgttatcc gctggcatct ggatagcggc ctggtggtga
    46381 tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt tgatgtctgg gatttccgtc
    46441 tcgacaaaga cgaactcggc aaaattgcaa aactcgatca gggcaagcgt ctcggtcccg
    46501 atcctgacca gttcggcggc taacgtgcaa attctcccgg tggcggtaat gttccgctac
    46561 cggacttttc agaaatcatt tattcccctc gcgtcccgcc cgttgttact cttccttgtt
    46621 caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa aagaacaact
    46681 catctatatc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat aatacttctg
    46741 tttttagcag ccctggcaag ttttaccgta cacgcgcagc ctccttcaca gaccgtagaa
    46801 caaacagtcc ggcagattta tcagaactat aaatcagatg ccagtacgcc ttatttcggt
    46861 gaaaccggag agcgggcgat aacttcagcg cgtattcaac aggcgcttac cctgaacgac
    46921 aatcttacac taccgggcaa tattggctgg ctggattatg atccggtttg tgattgtcag
    46981 gattttggcg atctggtgtt agaaagcgta gcgataaccc aaactgacgt cgatcatgcc
    47041 gatgccgttg tgcgctttcg tatctttaaa gatgataaag aaaagaccac acagacactg
    47101 aaaatggtgg cggaaaatgg tcgttgggtc attgatgata ttgtcagcaa tcatggcagc
    47161 gtcttacaag cagttaatag cgagaatgaa aaaacgctgg ccgctttagc ttcattgcag
    47221 aaagaacagc cagaatcctt tgttgctgaa ctctttgaac atattgctga ttatagctgg
    47281 ccgtggacgt gggtggtttc cgactcttac cgccaggcgg ttaatgcctt ctataaaacc
    47341 accttcaaga cggccaataa tcccgatgaa gatatgcaaa tagaacggca atttatttac
    47401 gacaatccga tctgttttgg cgaagagtcg ctattttcac gcgttgatga aattcgagtc
    47461 ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt ttacgctgac caatggcaac
    47521 aatgaagagc aagagctggt tttacagcgg cgcgaaggta agtgggaaat cgctgatttt
    47581 atccgcccga acagcggcag cctacttaag cagattgagg caaaaaccgc cgccagatta
    47641 aagcaatgag ctgaattaaa taacaattag ccggaacaat aaataaaagg gaacactata
    47701 tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc agtggctgcg
    47761 tcaatgagca aaaggtcaat caactggcga gcaatgtaca aacattaaat gccaaaatcg
    47821 cccggcttga gcaggatatg aaagcactac gcccacaaat ctatgctgcc aaatccgaag
    47881 ctaacagagc caatacgcgt cttgatgcac aggactattt tgattgcctg cgctgcttgc
    47941 gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt cagttgccgg ggattttttt
    48001 taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattcaccgg acgagattgc
    48061 gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gagtcgccat cggcgtatgt
    48121 ttagtcaacg ccggacgggt attgcggttc tggcgacgag cttcacgcat ctcttcaatg
    48181 gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt gcccatcgct
    48241 tccagcgcct gacggattaa cggccagttt gccggatcgt ggtaacgcaa caacgcttta
    48301 tgcaaacgac gctgtttgtc gcccttcggt acgaagacgc cttcgctctt ataaccaatc
    48361 ttcgccagcg gttttttccc ggtgtaatac atggtggttg agttcgccag cggcgatggg
    48421 tagaagttct gtacctggtc gagacggaaa cgatgctttt tcagccacag cgccaaattc
    48481 accatatctt catcacgcgt accggggtgc gcagagatga aatacgggat cagatactgc
    48541 tctttacctg cctgtttcga gtaggtatcg aacagctctt taaagcggtc atagctgccc
    48601 atgcccggct tcatcatctt cgacaacggc ccttcttcgg tatgttccgg agcaatcttc
    48661 agataaccgc cgacgtgatg ggtcgccaac tctttgatat agcgcggatc ttccacggcg
    48721 atgtcataac gtacgcctga ggcgataagg atttttttaa tgcctttcag atcacgcgca
    48781 cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca tgtgcggaca aatatccgga
    48841 taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg gcgatttgca gcgcaacata
    48901 tacatgttgg cagttggccc accgagatcg gaaatcacgc ccgtaaaacc tggaacggtg
    48961 tcgcggatcg cttcgatctc attaatgatc gaatcttccg aacggctctg aataatgcgc
    49021 ccttcgtgct cggtaataga acagaaagaa cagccgccaa agcagccgcg cataatgttg
    49081 accgaaaaac ggatcatttc gtaagccgga atacgggcat tgccataggc cggatgtggc
    49141 acgcgcttgt acggcagcgc aaaaacgctg tccatctctt cggtagaaag cgggatagca
    49201 ggcgggttga tccacacata gcggtcaccg tgtttttgca tcaatgcgcg ggcgcagcct
    49261 gggttggttt cgtggtgcag aatgcgcgaa gcatgggcgt acagcacttt atcgcccttc
    49321 actttctcga aagaaggcag caatacgtag gttttttccc acggtttctg gcgcggtggc
    49381 tgcacgatta cagctttcgc ttcctgcttt ttcggcgcaa caggtttgtt atccgcacac
    49441 ggcaaatctt cgccatacgg atgcgggatt ggggcgattt tccctggggt atcaagacgg
    49501 gtggaatcca caccgctcca gccaggcagc gcctctttta cgataattgc ggtattacgc
    49561 acatcgcgga tttcactaat cggctcgccc atcgccagac gatgcactac ttccaccagc
    49621 ggacgttcac cgttaccaaa catcagcatg tcggctttcg aatctaccag aacggaacgg
    49681 cgcacggtat cggaccagta atcataatgc gcggtacggc gcaggctagc ctcaataccg
    49741 ccgaggatca ccggcacatc tttccacgcc tctttacaac gctgggtata aaccagcgtg
    49801 gcgcgatccg gtcgcttacc cgcgacatta tctggcgtgt aggcatcatc atgacgtaaa
    49861 cggcgatcgg cggtatagcg gttgatcatc gaatccatgt tgccagcagt aacaccaaaa
    49921 aacagattcg gtttacccag acgcataaag tcgtctttgc tgctccagtc cggctgggcg
    49981 atgatcccga cgcgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc catcccgaag
    50041 cttgggtgat cgacatacgc gtcgccagta accaaaatga tgtcgcagct atcccagcca
    50101 agttgatcca tctcttcacg agacatcggc aaaaacggtg ccggtccaaa acaggcggcc
    50161 cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat agagctcatt
    50221 ttgcttccag aaatgataaa aaaataatca aaggccgggg attataagcc ggaacgaaag
    50281 agaaatcgaa aggtattcca tactcgccct cctcgggcga gtatgaagat tacggtaccg
    50341 gattgaccaa cagttgccca atcgagccac ggtccgccat ttccagcgtc tgactgttga
    50401 agtagaacgg gaagtgcgcc caggaaggct gaccgaaata aacaagcagc tccacttgtc
    50461 cgtcaaccca aacggtatct ttccagcctc tgtcttccgg gaacggcatc gcgccattca
    50521 cgttacggat ctggaacatt acgccttcaa tatggaacgc ttgcggctcg tccgcgcgta
    50581 ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc aatacggttg acgtcccaca
    50641 gctgtccatt aatacccggg tcatcaccca gactgatatc gcgactgcga attggcgaac
    50701 cagccatgat ttcagttggc agcaagcgca tcggaagact gtctgtgacc agcggcagaa
    50761 ggccggttgg gcgcagcgtt agcaccaagg tagaaaccag aatactggat ggctcaaaga
    50821 agccgcgaat acgatcaaca atgctcgccg cttcaccaca ggtgatcgac acttcatcgc
    50881 cgttgctcat atccaccaga atctcgcggc gctcgcccgg tgccagcgaa agttgcttca
    50941 ccgatacagg agcaggcagg aatccctgat cgccagaaat cacatgtaac gggcgaccat
    51001 cgctcatctg tagttgatag cgacgagagt tcgacgcgtt cagcagtcgc aagcgcaccc
    51061 agccacgcga gacttcaacg tacgggcttt gtacaccgtt aaccagcagc gtatcaccaa
    51121 caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc aaagttatcc agccgtttat
    51181 cctggataat gaccggaaaa tcatccacac cataatggtt ggggataggc agcgacttgc
    51241 tgacttcatc ttccaccagc cacattccgg caaggccgtt atagacctgc tgagccgtgc
    51301 ggttgggagt attggcgtga taccacagag tagctgcgtt ctggcgaata ggcagtacgg
    51361 gtgcccagtc agcgtttggc gacatcatcc gtgccggacc gcccatcagc gggcctggta
    51421 cctgtagccc ggccaccgtc attgagacat tttctgtcag gcggttgctg taaataagct
    51481 taacatcgtc gcccttccag acgcggatag tcggccccag gtaacgacca ttgattcccc
    51541 agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca gtcataaaca
    51601 gcggttgccc acggcgagat tcaagtagcg gcggaacggg tagcggttgc tgttgcccgg
    51661 ctgcgctggc cttcagggga acagcgcctg cacaaagtgc aatccccgat gcctgaatga
    51721 actgacgccg actgagtgac atatttgctc catgtaaaac tggctaaagt aataccgtgc
    51781 aggaaagtgt tccccgcaaa aatacagatt gccgacttaa acttttccgg cggcttcgcg
    51841 ttctgcgact tctttatcga gctcggcgat tttttgttcc attatcgaac gacaatgggc
    51901 agccagctca cgaacctgat ctttgccata ctgactgacg tcaattggcg gcagcatttc
    51961 gacaatcacc agaccgttgt gcagtcgatt aagattaatc ttattcgaag ttgtagagac
    52021 gcacacggga ataatcggga cgcccgccgc aattgccgcg tgaaatgctc cagtcttgaa
    52081 cggtagcagg ccgcgaccac ggctgcgggt tccttccggg aacatccaga tggaaatacg
    52141 gcgttttttg aaatgattca cgacttccgc aatggtgccg tgagctttag tgcgattgtt
    52201 tctgtcgatc aataagttgc cggttaacca gtacaactgc ccgaagaagg ggatccacag
    52261 caagctcttt ttacctaccg tcaccgtcgg cggttgcaca atgttcgatg ctgtcaccat
    52321 gtcatagttg ttctggtggt tagcgatata gatagcattg ccgtagtttt cagcatctgc
    52381 aggtttgcgg cactcaactt tcaggccgaa cagtggcgca agacggccaa acatatgccc
    52441 aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg agccgaatac
    52501 acagactaag atgctgtaaa tcacggtaat aataagacga aagatatata gcataacacc
    52561 ctctgaaggt ctgagagact ggcattgtac gtcacctgaa atcaggttag ggcattgtta
    52621 aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac aacggtttca
    52681 agggaatttt tatcgtttcc gggggttgaa tacgctgccg gatgacgact taacgtttca
    52741 tccggcgttc cttgctagcg ggaggaaaca gcgccctccc cggcatatta ctcttcgcta
    52801 tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg ctgcaaaccg
    52861 cgcatcaacg taccgcggcg tccacgttcg ccagtgactt tctgtagctc ttccggacgc
    52921 agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg cagaacgtac
    52981 agttgcgcca gaccatcctc gccacgcgcg gcttctgccg atggaatgtt gataatcttg
    53041 ttgcctttgc ccttcgacag ctgcggcaga tcacttaccg ggaacatcaa catacggcct
    53101 gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg cggcataaca
    53161 tgggcatttt ccggtaaggt gatcaaagcc ttacccgcac ggttacgcgc taccagatcg
    53221 ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt ctgatcgtcg
    53281 ctttccatca gcatatggtc aacggtcgcc ccaggcggca acgttaattt gccggtgagt
    53341 ggttcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata gctacgaccg
    53401 gtggaatcaa caaacactac cggttggtta ctcttacctt tcaccgccgc tttgaagcta
    53461 tcacccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc gctgcgtacc
    53521 cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc gtgctcgctc
    53581 atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gatcgtcacc gtaggcttgc
    53641 gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga agccaaaatg
    53701 ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat cttcatctct
    53761 tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg ggtttccgta
    53821 aggccaaacc gcgacatcag cgccggtttc ggttcatctt cgttacgaat gatctcaatc
    53881 acttcgtcga tgttgagaaa cgccaccagc aaaccttcca ggatatgcag gcgcttgagg
    53941 actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcgacggaa caccagccat
    54001 tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc gatcatattg
    54061 aggttaatgc gatagctctt ttccagatcg gtggtagcga agaggtggtt catcacctga
    54121 tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt ctcgtggtca
    54181 gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg cgcagcaatt
    54241 tgctccagta cgcgcgcacc tgaaacctga tgcggtaatg cgctgataac caccgcgcca
    54301 tcttctttct tccacaccgc gcgcatacgc actgaaccac gtccgttctc gtagatttta
    54361 cgaatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg cccctgcacg
    54421 atatccagca gctgatcgag cgtagttttc ggctggtcga ttaatgcgat tgccgcctga
    54481 gctacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc aataccggtg
    54541 gtgccgttaa gcaaaatgtt tggcagacgg gcaggtagca ttttcggctc ctgcaaagtg
    54601 ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa tagcagctcg
    54661 gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt cggatcgtcc
    54721 ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa cggctgcgcc
    54781 atcaggacca tcgcttcata acaggcgcta tcgccgtgcg gatggtattt acccagtacg
    54841 tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc cagttcagac
    54901 atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa cggcaacgca
    54961 cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa ttcatgtagc
    55021 gcaaggcgct ctgccatatc gctcattaat tctgattcct caacttattc gccagccttc
    55081 aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac aggagatcag
    55141 cgtgaatagc gccgccggac gtgctccgac ggcgataaag gcatcaatgt gccttgatat
    55201 acaactcttt gctgtagact tgatcggcat agagggcagc acaaggttgc gcccatgcca
    55261 caccactcgg catgggggtc cgctccgggc gattcgagaa gttgaggtta tgcgagtcca
    55321 gggaagccta aagcatcaaa gtagtcgact gtaagtatac ggttcagccc agtttagctg
    55381 tgtctccacc agtgccccgc gcctcctgcc ataagtgcgg cttgctcatg gatatccccg
    55441 agacggctta cggcgcaccg gataataaat ttcgcgtata catggtattc cttaccggag
    55501 aaattataag cccaacaaca atacgcctgt gacattgttc gctcaaactt atcgcgagtg
    55561 atttggctca cactccggta aagattcccc atcacgccaa cggcacataa acatccgttt
    55621 ccagtcgctg ctctgtcacc ccttcggcaa gattggtgta gtgaaacaga atcggtgctt
    55681 tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat aatgccccat atcgtgtgtg
    55741 aaatatcgtc cagctcgcca acgtggcggg ccacggcata acgtccaccg gtaagttcac
    55801 cattgctcac accataacga ttatcgggaa tcggttcgct aacgctgccg cagatatcaa
    55861 agcgaaacgc ttccggtgcg gtggtggcag gggcgtccca ggcgacgcca aaagtctggc
    55921 tttggttaac aggagaaagc cccgtctctt tacgccacat gataaatttc gccacactgt
    55981 agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc cacagggatt gatgggaaat
    56041 cgataatgtt tacatccagt gtcaggtttg tcattttgcc tccctgcgaa attattcccg
    56101 ggaacgcgtc ccgggagcgg taacaattac ggatttactt tgcggatctg tttgacgtca
    56161 atttcaacag aattccagtc tttatcgact tcaccctgaa tctcaaccgt atctttcggc
    56221 gtcaccgtca caccgttcca gcgtttgtga tcgatatcaa cattgatagt accgctggca
    56281 tctttaaaca cgtagagatc gtcagagatg cgttcaacga tattgccgcg cagggttacc
    56341 caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg ttacgctgcc gttcggcccc
    56401 tgaaatcctc cggcctgact ttgcgttacc gatgggccag aaaaaccgcc ctgctctgct
    56461 gccatcaccg gtgcactgca cagggccatt actgcgatta ctgctgcgaa ttttttcatg
    56521 tttattactc cctttaatgt ctgtttccga gcatttaaca agatagtcct taacaacttc
    56581 ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg ttacaacacg gtttactggc
    56641 agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt tactgataga agatgacatg
    56701 ctgattggtg acggcatcaa aactggcctt agtaaaatgg gttttagtgt cgactggttt
    56761 acacaaggtc gtcagggaaa agaggcgctt tatagcgcac cttatgatgc ggtgatcctg
    56821 gatttaacct taccaggcat ggatggtcgc gatattttgc gcgaatggcg agagaaaggt
    56881 cagcgtgagc cggtactgaa cctgaccgcg cgtgatgcgt tagcggaacg tgtagaaggg
    56941 ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg cgttgataga agtcgccgcc
    57001 aagctggagg ctctgatgcg ccgaaccaac ggccaggcca gcaacgagct gcgccacggt
    57061 aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg ctggcgaacc cttaacactg
    57121 aaaccaaaag aatttgccct gctggaatta ctgatgcgta acgctggtcg ggtactgccg
    57181 cgcaaactga ttgaagagaa actgtatacc tgggacgaag aggtcaccag taatgccgtt
    57241 gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg attttattcg taccgtgcat
    57301 ggtattggct acacattagg tgagaaatga aatttaccca acgtcttagt ctgcgcgtca
    57361 ggctgacgct aatcttttta attctggcct cggtgacctg gctgctttcc agctttgtcg
    57421 cctggaaaca aacaacggat aacgtcgatg aattgttcga cacccaactg atgctgtttg
    57481 ccaaacggtt aagtacgctc gatctcaacg aaatcaacgc ggcggatcgc atggcacaga
    57541 cgccaaataa attaaaacac ggtcatgttg atgacgatgc gctgaccttt gccatcttta
    57601 cccacgacgg cagaatggtc cttaatgatg gcgataacgg agaagatatt ccctatagct
    57661 atcaacggga aggttttgct gacgggcaac tggtcggtga agacgatcct tggcgttttg
    57721 tctggatgac ctcacctgat ggcaaatatc gcatcgttgt tggccaggaa tgggaatacc
    57781 gtgaagacat ggcgctggcg attgttgccg ggcaattgat cccgtggctg gtcgcactgc
    57841 cgattatgtt aatcatcatg atggtactac tgggtcgtga actcgcgccg ctgaacaaac
    57901 tggcgctggc actacgtatg cgtgaccctg actcggaaaa accactaaac gcgactggcg
    57961 tacccagcga agtgcggcca ctggttgagt cgctaaatca actgttcgcc cgcacacatg
    58021 cgatgatggt tcgagaacga cgctttacct ccgatgcagc tcacgaacta cgtagcccgt
    58081 taacagcgct gaaagtgcaa accgaagttg cgcagctctc tgacgatgat ccgcaggcgc
    58141 ggaaaaaagc actgctccaa ttacattccg ggatcgatcg cgctactcgt ctggttgatc
    58201 aactgctcac gctatcgcgg ctggactcac tggataacct tcaggacgtc gcggagatcc
    58261 cgcttgaaga tctcctacaa tcgtcggtga tggatattta ccacacggcg cagcaggcga
    58321 aaattgacgt gcgactgaca ctcaatgccc acggcatcaa acgcaccgga caaccgctat
    58381 tgctaagttt gttggtgcga aatttgctgg ataacggccc cggtgtgaca ccagaggcac
    58441 tggcgcgaat tggcgaacgc ttctatcgcc cacccggaca aaccgctacc ggcagtggac
    58501 ttgggctatc gattgtccag cgaatcgcca agctgcatga catgaatgtt gagtttggga
    58561 atgcggaaca aggtggattt gaggcgaagg taagctggta acttctctgt ctatgagagc
    58621 cgttaaaacg actctcatag attttactaa tagcaaaata taaaccgtcc ccaaaaaagc
    58681 caccaaccac aaaccagaca aataacgagc gagaatacat atagccaaaa ctcgaacatt
    58741 tcccgcataa agagttttct taagataaga ataataagtg gcgtaagaag aaaaaatgct
    58801 gcaatcattg cttgcaatat acgatcttcg cctgaaacgg aaggtatact gcaaatattg
    58861 cctcccgact caagcatcca gtcatactta ttgaaagcaa aaaaagaatc tattaaccaa
    58921 taagtaagca ttatatatag caatgcttca aatattgttt taattttctg ctttaacata
    58981 tatcagtacg ctcataaaaa tccatttata attatcaaca tgacacgttg atattttcat
    59041 cttttactta ttcaaagcca tattttcaat ccccaaaaaa accgaaccaa acgtgtaaat
    59101 tattccggtg cgagtaagaa ttatgaggaa tggctatcag tattgtcatt ttcagaaaat
    59161 atttatcctg catcggtgag tcagagctag atcagacttt tgctaaattc gcaaaagact
    59221 ttgcacattt tgctaatttc acagtaccgc tctgtgacgt actatagtcg acaaacgtct
    59281 caccttgaga ttaaaaaatg agcaacatcc tgattatcaa cggcgcgaaa aaattcgccc
    59341 actccaatgg tcaactgaac gacaccctga ccgaagtcgc ggatggcaca ctgcgcgacc
    59401 tcgggcatga tgtccgcatc gttcgcgccg acagcgacta caatgtcaaa gcggaagtac
    59461 aaaactttct ctgggctgat gtggtgatct ggcagatgcc aggctggtgg atgggcgcgc
    59521 cgtggacagt gaaaaaatat attgatgatg tattcaccga aggtcacggg actctgtatg
    59581 ccagcgatgg tcgtacccgc aaagatccgt cgaaaaaata cggttccggc agcctggtac
    59641 agggcaaaaa atatatgctt tctctaacct ggaacgcacc aatggaagcc ttcaccgaaa
    59701 aagatcagtt cttccacggc gttggcgttg acggtgtgta tctgccgttc cataaagcaa
    59761 accaattcct cggtatggaa ccgctgccga catttatcgc taatgacgtg ataaaaatgc
    59821 ctgatgttcc ccgctatact gaagaatatc gcaagcatct tgtggaaatt tttggttaac
    59881 tagagctcag gctttagaag gagttaacca tgcttaccgt aatcgcagaa atccgtactc
    59941 gtcctggtca acatcaccgt caggcggtat tggatcagtt tgctaaaatc gttccaaccg
    60001 tactgaaaga agaaggttgc cacggctatg cgccaatggt ggattgcgct gctggcgtga
    60061 gtttccagtc tatggcaccg gattctatcg tgatgattga gcagtgggaa agcatcgcgc
    60121 accttgaagc gcatctgcaa accccgcaca tgaaggcgta cagcgaagcc gtaaaaggcg
    60181 acgtgctgga gatgaatatc cgtattctgc agccagggat ttaatcctgc cttgtttgcc
    60241 cggccatcct gaccgggcaa tgttctttcc tttaaacctc aatctccgcc atgtcgcctt
    60301 tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt cgccagcagc atatccatca
    60361 tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa ctgcaccaga cggcgagtgt
    60421 tcggatcaag cgtggtttca cgcaattgca tcgggttcat ttcccccaga cctttaaaac
    60481 gctggacgtt cggcttgcct ttcttgcgtt ttaattgctc aagtacgccc tctttctctt
    60541 cttccgtcag cgcgtaataa acctctttcc cgagatcaat acggtagagc ggtggcagtg
    60601 cgacgtaaac gtgaccgtgt ttcaccaacg cgcggaagtg ttttacgaac aaagcgcaga
    60661 gcagcgtggc aatgtgcaga ccatcagagt ccgcatccgc gaggatacag attttgccat
    60721 aacgaagctg gctcagatcg tcgctgtcag gatcgatacc gatcgctacc gaaatatcat
    60781 gcacttcctg cgaggccagc acttcgtcgg aagaaacttc ccaggtgtta aggatcttac
    60841 ctttcagcgg catgatcgcc tgatattcac gatcgcgcgc ctgcttggca gatccgcctg
    60901 cggagtcacc ttccactagg aacagttcgg tacggttaag gccctgcgcg gtacaatcag
    60961 ccagtttgcc aggcagcgcc gggccgctgg tcagcttttt acgcaccact tttttagccg
    61021 cacgcatacg acgctgggcg ctggaaatcg ccatctctgc cagcaactcc gccgcctgaa
    61081 cgttctggtt cagccacagg atgaaggcat ctttcaccac gcccgaaacg aatgccgcac
    61141 actgacgcga agagagacgc tctttggtct gcccggcaaa ctgcggatcc tgcattttaa
    61201 ccgacagaac ataggcgcag cgatcccaga tatcttccgc cgacagcttc acgccgcgcg
    61261 gcagaatgtt gcggtattca cagaactcac gcatcgcgtc caacaggccc tgacgcagac
    61321 cattaacatg ggtaccgccc tgcatcgttg ggataaggtt gacgtagctt tcggtcagca
    61381 gttcaccgcc ttccggcagc cacagtagcg cccagtccac agcttcagta tcgccagcga
    61441 aattaccgat aaacggtttt tctggcagcg tcggtaaacc gtttaccgct tccgccaggt
    61501 aatcattcag accgtcctga tagcaccagc gctgttcggt gttgttgatc tcatctttaa
    61561 aggtaatctc aacgccgggg cacagtactg ctttggcctt cagcacatgc gtcaggcgtg
    61621 aaacagaaaa acgcgggctg tcaaagaagg tttcatccgg ccagaagtgc acgctggtac
    61681 cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc ctgcaccttt tcgccatttt
    61741 caaaggcgat gttataaacc tgaccatcgc ggcgcacgtt aacttctacg cgcttcgaca
    61801 aggcgttaac taccgaaatc cccacgccat gcaggccgcc agagaactgg taatttttgt
    61861 tagagaattt accgcctgca tgcagacggc aaagaatcag ttcaaccgcc ggtactcctt
    61921 cttccgggtg aatatccacc ggcatcccgc gcccatcgtc aatcacttct aacgactggt
    61981 cagcatgtaa gattacatcc acgcgttttg cgtgacccgc cagtgcttca tcgacactgt
    62041 tatcaataac ttcttgcccc aaatggttag ggcgagtggt atcggtatac atccccggac
    62101 ggcggcgaac cggctcaagc ccggtgagta cctcaatggc atcagcgtta taagtttgcg
    62161 tcatggttta agttagcaat tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg
    62221 acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca
    62281 gtctggcggc aggaagcgta gtacgccacc gcctggcggt aatccagcac ttcatctccc
    62341 gtctgttgca gcagccagat caaatccggc gcttccagcg ggtcaatctg catgactttg
    62401 agatcgtaaa tatggcgtga ctctagcaca tattgctgcc cggtgtaggg gttctcgttc
    62461 tgaccgagat agtccgtcag caattcaaac gggcgtaccg ccgggtttac caccactgcg
    62521 ggcagcataa aacattgcga caaccaggtg gcgtaatatc cccccagtga cgaaccgaca
    62581 atacccagcg aatcgccgcc atgttccagg acaatggatt ccagcagttc tgccgcgtcg
    62641 gaaggatacg gcggcaactg cggaatgatc atctcaacgt cagggtgatg ttccgccagc
    62701 cagtttttta acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa
    62761 agaagcgtag acatcagtag ccttctgaag cggtatcagg ttggaaacgt gtgtccgcca
    62821 ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta cgccagccgg
    62881 gcgcgatagt atccagcgta aagttggaac agtgcggctt aaactgcaca caggtcgacg
    62941 gcgttgccag caggcggcga ccgttccagt cgagatccag ctcctgatga atatgaccgc
    63001 acagcaagta tttgacgtgc ggaaacttcg ccagcacggt atccagttcg cccgcgttac
    63061 gcagactgtg ttgatcgagc caactacaac ccgcaggtag cggatgatga tgcagcagca
    63121 gcaacgtatg gcgttctggc gcatcggcca gtttacgttc cagccactca agctgaaact
    63181 cgctcagctc accgtgcggc acgccaaaca cctggctatc cagcaacagg atttgccatt
    63241 gctcaccaat aaacacccgc ttcgccgggg agatacccgc atcctgtaac gcgctgtaca
    63301 tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca gggcgcacga aaacttgcga
    63361 tgccttcagc gaaatgctga taggccgcag aggattgatc ctgcgctaaa tcacctgtcg
    63421 cgacaatcag gtcgaattcg tgctggtgtg gacgaatcgc ctccagcacc gcctggtagc
    63481 tttcccaggt gtttacccct aacagggctt cgtgcttttg tgcaaacagg tgagtatcgg
    63541 taatttgtaa aatccttcct ctggcctcac cagccagagg aagggttaac aggctttcca
    63601 aatggtgtcc ttaggtttca cgacgctagt aaaccggaat cgccatcgct ccatgtgcta
    63661 aacagtatcg caaccagtcc gctaaaaact ggttaatttg atgcttttcg tcgcgttgat
    63721 gcaacttttt attaggataa tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac
    63781 acacttcagc caccatcgcg tcatgataca gacgcaccgt cattgacgga aggctccagt
    63841 aactgatcgc gggcgcagtc tgttctattg tcactagggt agtgtatcgg gtcgattcca
    63901 caatcgtcag ccgatattgt gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt
    63961 cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt
    64021 caggaaagtc aggtgtgtaa cgcttcattt atgcccactc attttttaac gcttgatgat
    64081 gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta
    64141 cccactggta tgcctgttcc cggcttacca catgaacgcg aatatcttcg ttttcatcag
    64201 ccagaccatg aataccgctt gcggtcgtgg cgtccacttc gcccaccata attgacgaac
    64261 gctcactggt gccccccggg cttgctagga aacttaacac cggtttggtc cgtttgacta
    64321 tcagtcccgc ctcttcaatc gcttcgcgac gggcaacatc ttccacactt tcaccctctt
    64381 caatcatccc ggcaaccatc tccagtagcc agggggtttc gctggtgtcg tacgcggcaa
    64441 tccgaatctg ctcaatcagc acaacttcat cacgcactgg gtcaaagggt agcaagactg
    64501 cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg actcatttgc ccgttgaata
    64561 gacgatgacg aaatctataa agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg
    64621 caataatttc tacatcgttt ttgccaaatg taacgggcag gttgtctggc ttaagcattg
    64681 ttaatgtcct agcactaata gtgaattaaa tgtgaatttc agcgacgttt gactgccgtt
    64741 tgagcagtca tatgttaaat tgaggcacat taacgcccta tggcacgtaa cgccaacctt
    64801 ttgcggtagc ggcttctgct agaatccgca ataattttac agtttgatcg cgctaaatac
    64861 tgcttcacca caaggaatgc aaatgaagaa attgctcccc attcttatcg gcctgagcct
    64921 ttctgggttc agttcgttga gccaggccga gaacctgatg caagtttatc agcaagcacg
    64981 ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat
    65041 taatgaagcg cgcagtccat tactgccaca gctaggttta ggtgcagatt acacctatag
    65101 caacggctac cgcgacgcga acggcatcaa ctcgaacgcg accagtgcgt ccctgcagtt
    65161 aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc
    65221 agggattcag gacgtcacat atcagaccga tcagcaaacc ttgatcctca acaccgcgac
    65281 cgcttatttc aacgtgttga atgctattga cgttctttcc tatacacagg cacaaaaaga
    65341 agcgatctac cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat
    65401 caccgacgtg cagaacgccc gcgcgcagta cgataccgtg ctggcgaacg aagtgaccgc
    65461 acgtaataac cttgataacg cggtagagca gctgcgccag atcaccggta actactatcc
    65521 ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc cggttaacgc
    65581 gctgctgaaa gaagccgaaa aacgcaacct gtcgctgtta caggcacgct tgagccagga
    65641 cctggagcgc gagcaaattc gccaggcgca ggatggtcac ttaccgactc tggatttaac
    65701 ggcttctacc gggatttctg acacctctta tagcggttcg aaaacccgtg gtgccgctgg
    65761 tacccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct tctcgctgcc
    65821 gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcacagtaca actttgttgg
    65881 tgccagtgag caactggaaa gcgcacatcg tagcgtcgtg caaaccgtac gttcctcctt
    65941 caacaacatt aatgcttcta tcagtagtat taacgcctac aaacaagccg tagtttccgc
    66001 gcaaagctca ttagacgcga tggaagcggg ctactcggtc ggtacgcgta ccattgttga
    66061 tgtgttggat gcgaccacca cgctgtacaa cgccaagcaa gagctggcga atgcgcgtta
    66121 taactacctg attaatcagc tgaatattaa gtcagccctg ggtacgttga acgagcagga
    66181 tttgctggca ctgaacaatg cgctgagcaa accggtttcc actaatccgg aaaacgttgc
    66241 cccgcaaacg ccggaacaga atgctattgc tgatggttat gcgcctgata gcccggcacc
    66301 cgtcgttcag caaacatccg cacgcactac caccagtaac ggtcataacc ctttccgtaa
    66361 ctgatgacga cgacggggct tcggccccgt ctgaacgtaa ggcaacgtaa agatacgggt
    66421 tatctgccgc attcttcccc cttctcgctt caatttcgac cagccatcct ctattctgat
    66481 gggtatttac cactggtccc ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc
    66541 atcgttccgc aaaaactgga gcgcacgcca tctgacacca gtcgctctcg cggttgccac
    66601 tgtttttatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc
    66661 tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa
    66721 tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc
    66781 tgaatttggt gaaggtcagt gtcagcaagc accagcccag gctggcatgg caccagaaaa
    66841 ccaggcgcag gctcagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat
    66901 gatggggcgt ctgatgggcg gcggcgcggg atttgcacag cagccgctgt tctcctcgaa
    66961 aaacccggcc agtccggctt acggtaaata taccgacgcg acgggtaaaa actatggcgc
    67021 agcccagcca ggccgcacca tgaccgtacc gaagacggca atggcaccaa aaccggcgac
    67081 caccactacc gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca
    67141 gcgtagcgca accggcacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc
    67201 agtattaccg agcgcccgga ctggcgtgag aaagcccacg aatacggttt caattttcac
    67261 accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac cctcgcccag
    67321 gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa
    67381 aaagtgatcg ccagcgatga gctgatgacc aaattccgca taccaaaaca cacctggagt
    67441 tttgtgcgtc aatcatggct gactcaccag ccatcgcttt attcgcgtct tgatctggcg
    67501 tgggatggca ctggcgaacc taaacttctg gaaaataacg ccgatacgcc aacgtcacta
    67561 tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg
    67621 tcggagggca gcgaccagtt taacagtctg caagaaaaac tgatcgatcg cttcgttgag
    67681 ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa
    67741 gatcgcggaa ccattcagta tttgcaggac tgcgcaaccg aagctgaaat cgccaccgag
    67801 ttcctctaca tcgacgagat aggtttaggc gaaaaaggcc agttcacgga tttacaggat
    67861 caggtgattt ctaacctgtt caagctttat ccgtgggaat ttatgctgcg cgaaatgttc
    67921 tccaccaagc tggaagatgc tggcgtacgc tggctggaac cggcgtggaa gagcattatc
    67981 tccaacaagg cacttctacc gctactgtgg gagatgttcc cgaatcaccc gaacctgctg
    68041 cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taaaccgatc
    68101 ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg
    68161 gaaggtccgt atggcgaaga agggatgatt gttcagcaat tccacccgtt accgaaattc
    68221 ggcgacagct atatgctgat tggtagctgg ctggtgaacg atcaacctgc cggaattggc
    68281 attcgtgaag accgtgcgtt gatcacccag gatatgtctc ggttttatcc acatattttt
    68341 gttgaataag ccacgatacc ggatggcact cgccatccgg taattgttag cctatctgca
    68401 ccgacagcat acttaaactg cccatttcta taccatcgac cggaatggta attggctcct
    68461 gcccatccca cacacctaac acatacaaca acggcagata gtgctctggc gttgggttcg
    68521 ataacgcgcc accttcatgg tcgagataat tcaccagagg atgctgctct accggccctt
    68581 gccacgtcag attcgctttc acatactcat taaacgacgt cgcccacgga tacggtgaac
    68641 tatcaccgtg ccacttcact gtgcgcaggt tatgcaccac gttaccgctg gcgaccaaca
    68701 ttattccttc atcacgcagc gccgcgagtt tgcgccccat ttcgaaatgc caggcgacag
    68761 gtttgctact gtcgatactc aactgcacca tagggatatc ggcgtcagga tacatcttaa
    68821 tcagcacgcc ccacgagccg tggtcaaagc cccaggcttc tttatccagc gtcaccggga
    68881 tcggcgctaa cagctcaacc agacgctgtg ccagcgcagg cgaacccgga gcaggataat
    68941 gcgtatcgta cagcgcctgc gggaagccac caaagtcatg aatcgtgggc ggcgtctcca
    69001 tcgcggtcac tcctgttcca cgggtaaacc agtgagccga aaccaccaca atcgcttgcg
    69061 ggcgtggcaa tgtcatcccc aacttctgcc agctgcgggt atacaaatta tcttccagca
    69121 cgttcatcgg actaccgtga cctaaaaaca atgctggcat acgtgttgaa gacatgatga
    69181 tatccttaac taaaggtgtc attttgatat cctcacaata cgcttgttcg gcggagtaag
    69241 aacccggata acaatgatga tgatcatcag ttattttgac gatctgcctg aaggtgaaga
    69301 tttataagga gttgtcgatg tcagtacctc tcattctgac catactggcg ggggcagcca
    69361 cgtttattgg cgcgtttctc ggcgttctcg gacaaaaacc ctcgaaccgc ttactggcgt
    69421 tttcgctagg ttttgcggcg gggatcatgt tgctcatctc attaatggaa atgcttcctg
    69481 ccgcactagc agctgaagga atgtcgccgg tgttgggtta tggaatgttt atcttcggtc
    69541 tgcttggcta ctttggcctg gaccgcatgt tgccgcatgc gcatccgcag gatttaatgc
    69601 aaaaatcggt gcagctgttg ccaaaatcga tcaagcgcac agccattctg ctcactctcg
    69661 gcatcagtct gcataacttc ccggaaggga ttgccacctt tgtcacggcg agcagcaacc
    69721 tggagctggg atttggcatc gcactggccg tcgcgttgca caatatccct gaaggtctgg
    69781 cagtggcagg cccggtttat gcggcaacgg ggtctaaacg caccgcgatt ctgtgggcgg
    69841 ggatttctgg actagcagaa attcttggtg gtgtgctggc gtggttaatc ctcggtagca
    69901 tgatttcccc ggtggtcatg gcggcaatca tggcggcggt tgcaggaatt atggtggcgc
    69961 tctcggttga tgaattaatg ccgctcgcca aagagattga ccctaataat aaccccagct
    70021 atggcgtact atgtggaatg tcagtgatgg gattcagttt agtgctgcta caaacggcgg
    70081 gaattggtta aaaacaacat cagaagttgt ccagcacatg gacaacttct gccagcttca
    70141 cgaaagcctt attctacctt ttcggctgtc tcttctctcg tactgtttaa gtatttgttc
    70201 cgcgcttcaa gcagtgcgtc tctgtcctgg cgaaacgccc gacaataacg tttcaaatgc
    70261 aggaaataac ccaccacaac cagagcaatc aatacaatcc agtaccaggc gataaacatc
    70321 cttttaccct taatatttaa caatactaaa catttagcgc ataaatttca catatccttt
    70381 ttcggatata ttcatatggt cgcataataa cggaacaatt atatgatttt gatcataatg
    70441 aaaaagaatg cctgatatta atgaaatttt ctcatagaaa gagaagcgga attaacatct
    70501 tgcatagcag ggcaaaataa aaaccgaagc cagctacaac ttcggtttct tgattaaggc
    70561 agtaattaag cagcggtttt cagctggctt tacgctcatg tgcctgacgg tatgccacca
    70621 ggtcttcaat agtcacgagc gccatattgt gtttattggc aaactcaatg cactctggtg
    70681 cacgcgccat cgtgccatcg tcattagtca gctcacacag cacaccagcc ggtttaaagc
    70741 ctgccagcgt catcaaatca atagtcgctt cagtatgacc gccacgcgtc agtacaccac
    70801 ctgcctgcgc gcgaagtggg aaaacgtggc ccgggcgatt cagatcagaa ggttttgcgc
    70861 catcggcaat cgctgcgcga acggtcgtaa tacgatcagc ggcagaaaca ccggtagtca
    70921 caccttcagc cgcttcaatg gtcacggtaa aaccggtgcc ataggcgctg gtgttatttt
    70981 ctaccatcat tggcagatca agttgtttac ggcgatcttc agtgatgcac aggcaaacaa
    71041 taccgctacc gtggcgaatg gtcagcgcca tctgctcaac ggtcatggtt tctgccggga
    71101 agatcatatc gccttcgttt tcacggtctt catcatcaag caccattaca ccgcgtcctt
    71161 cacgcagcgc agccagtgca ttttcaacac gttcgaaagg cgtaccaaaa gaggaaagta
    71221 gcgtctgatt catggtaaaa aaacctcact aaaattatgg ttaccagaat cagggcagtc
    71281 ttaggagtgg cggcatatag ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt
    71341 actctctccc atccggactc taaccgtcgg ccccggaatt acaccggatc tgctgtcctt
    71401 tgagttcgca cccaaagcgc tcgcgggctt tcaactgagt tgatttaccg ccggtgggga
    71461 atttcgcccc gccctgagaa taagcggatt cactataacg ctaatgatta gcggcagcaa
    71521 cgcatagctt cacataattc tggtttatga cttaccctta tcgcactaca atggtactca
    71581 acacctatca gtacagggaa actacaatga ttgacccgaa aaaaattgag caaatcgctc
    71641 gccaggttca cgaatcaatg cctaaaggaa tcagggagtt cggggaagat gtggagaaaa
    71701 aaatccgcca aaccctacaa gctcagctga cgcgcctcga ccttgtaagc cgtgaagagt
    71761 tcgacgtcca aacgcaagtc ctgttacgga ctcgtgagaa actggcgctg cttgaacaaa
    71821 gaatcagcga actggaaaat cgcagtactg agatcaaaaa gcagccagat ccagagactc
    71881 tccccccaac gctgtaaaca tttgtcttta atgcactgta aattatgaat taatcctcta
    71941 atttacagtg ttttctatta cattatcgct taatcccatt cagagtatca tcacaaaaat
    72001 ga
//
LOCUS       DAJTNV010000005        70706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNV010000005 DAJTNV010000000
VERSION     DAJTNV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..794
                     /locus_tag="OO199_000302"
     CDS             <1..794
                     /locus_tag="OO199_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5534485.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OO199_000303"
     CDS             805..1896
                     /locus_tag="OO199_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534486.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO199_000304"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO199_000304"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5534487.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OO199_000305"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OO199_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5534488.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OO199_000306"
     CDS             4858..5253
                     /locus_tag="OO199_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5534489.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OO199_000307"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OO199_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5534490.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OO199_000308"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OO199_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5534491.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OO199_000309"
     CDS             complement(6562..7602)
                     /locus_tag="OO199_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5534492.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OO199_000310"
     CDS             complement(7675..8310)
                     /locus_tag="OO199_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5534493.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OO199_000311"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OO199_000311"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5534494.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OO199_000312"
     CDS             complement(8936..9379)
                     /locus_tag="OO199_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5534495.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO199_000313"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO199_000313"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5534496.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OO199_000314"
     CDS             complement(9719..10222)
                     /locus_tag="OO199_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5534497.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OO199_000315"
     CDS             complement(10216..10740)
                     /locus_tag="OO199_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5534498.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OO199_000316"
     CDS             10949..11944
                     /locus_tag="OO199_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5534499.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OO199_000317"
     CDS             11953..12831
                     /locus_tag="OO199_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5534500.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OO199_000318"
     CDS             12912..13919
                     /locus_tag="OO199_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5534501.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO199_000319"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO199_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5534502.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO199_000320"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO199_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5534503.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO199_000321"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO199_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5534504.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO199_000322"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO199_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5534505.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO199_000323"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO199_000323"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5534506.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO199_000324"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO199_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5534507.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO199_000325"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO199_000325"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5534508.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO199_000326"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO199_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5534509.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO199_000327"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO199_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5534510.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO199_000328"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO199_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5534511.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO199_000329"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO199_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5534512.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="OO199_000330"
     tRNA            complement(27653..27729)
                     /locus_tag="OO199_000330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="OO199_000331"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="OO199_000331"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5534513.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="OO199_000332"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="OO199_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="OO199_000333"
     tRNA            complement(31513..31599)
                     /locus_tag="OO199_000333"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO199_000334"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO199_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5534514.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO199_000335"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO199_000335"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5534515.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO199_000336"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO199_000336"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5534516.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGMHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO199_000337"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO199_000337"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5534517.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO199_000338"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO199_000338"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5534518.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="OO199_000339"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="OO199_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5534519.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO199_000340"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO199_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5534520.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="OO199_000341"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="OO199_000341"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5534521.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO199_000342"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO199_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5534522.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="OO199_000343"
     CDS             complement(41211..42176)
                     /locus_tag="OO199_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5534523.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO199_000344"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO199_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5534524.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO199_000345"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO199_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5534525.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="OO199_000346"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="OO199_000346"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5534526.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="OO199_000347"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="OO199_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5534527.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO199_000348"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO199_000348"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5534528.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO199_000349"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO199_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5534529.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO199_000350"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO199_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5534530.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO199_000351"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO199_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5534531.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO199_000352"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO199_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5534532.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO199_000353"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO199_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5534533.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO199_000354"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO199_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5534534.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="OO199_000355"
     CDS             49714..50679
                     /locus_tag="OO199_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5534535.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="OO199_000356"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="OO199_000356"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5534536.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="OO199_000357"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="OO199_000357"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5534537.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="OO199_000358"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="OO199_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5534538.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="OO199_000359"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="OO199_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5534539.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="OO199_000360"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="OO199_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5534540.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="OO199_000361"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="OO199_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5534541.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="OO199_000362"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="OO199_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5534542.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="OO199_000363"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="OO199_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5534543.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="OO199_000364"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="OO199_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5534544.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="OO199_000365"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="OO199_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5534545.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="OO199_000366"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="OO199_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5534546.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OO199_000367"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OO199_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OO199_000368"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OO199_000368"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5534547.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OO199_000369"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OO199_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5534548.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="OO199_000370"
     CDS             complement(62540..63469)
                     /locus_tag="OO199_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5534549.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="OO199_000371"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="OO199_000371"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5534550.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="OO199_000372"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="OO199_000372"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5534551.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="OO199_000373"
     CDS             70219..70698
                     /locus_tag="OO199_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5534552.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17316 a        19178 c        17550 g        16662 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcatgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaagga
//
LOCUS       DAJTNV010000006        67237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNV010000006 DAJTNV010000000
VERSION     DAJTNV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..228
                     /locus_tag="OO199_000374"
     CDS             <1..228
                     /locus_tag="OO199_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5534553.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OO199_000375"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OO199_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="OO199_000376"
     CDS             1366..1689
                     /locus_tag="OO199_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5534554.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO199_000377"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO199_000377"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5534555.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO199_000378"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO199_000378"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5534556.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO199_000379"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO199_000379"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5534557.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO199_000380"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO199_000380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5534558.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO199_000381"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO199_000381"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5534559.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO199_000382"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO199_000382"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5534560.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO199_000383"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO199_000383"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5534561.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO199_000384"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO199_000384"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5534562.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO199_000385"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO199_000385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5534563.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO199_000386"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO199_000386"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5534564.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO199_000387"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO199_000387"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5534565.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO199_000388"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO199_000388"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5534566.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO199_000389"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO199_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5534567.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO199_000390"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO199_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5534568.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OO199_000391"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OO199_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5534569.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OO199_000392"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OO199_000392"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5534570.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OO199_000393"
     CDS             complement(21228..23060)
                     /locus_tag="OO199_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5534571.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO199_000394"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO199_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5534572.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OO199_000395"
     CDS             complement(23808..24302)
                     /locus_tag="OO199_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5534573.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OO199_000396"
     CDS             complement(24385..24840)
                     /locus_tag="OO199_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5534574.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OO199_000397"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OO199_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5534575.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OO199_000398"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OO199_000398"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5534576.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OO199_000399"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OO199_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5534577.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO199_000400"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO199_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5534578.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO199_000401"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO199_000401"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5534579.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO199_000402"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO199_000402"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5534580.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OO199_000403"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OO199_000403"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5534581.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OO199_000404"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OO199_000404"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5534582.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OO199_000405"
     CDS             33416..34309
                     /locus_tag="OO199_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5534583.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO199_000406"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO199_000406"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5534584.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO199_000407"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO199_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5534585.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OO199_000408"
     CDS             complement(36225..36941)
                     /locus_tag="OO199_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5534586.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO199_000409"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO199_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5534587.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO199_000410"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO199_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5534588.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO199_000411"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO199_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5534589.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO199_000412"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO199_000412"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5534590.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO199_000413"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO199_000413"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5534591.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO199_000414"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO199_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5534592.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO199_000415"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO199_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5534593.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO199_000416"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO199_000416"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5534594.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO199_000417"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO199_000417"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5534595.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OO199_000418"
     CDS             complement(45790..46449)
                     /locus_tag="OO199_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5534596.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO199_000419"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO199_000419"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5534597.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OO199_000420"
     CDS             complement(47344..48357)
                     /locus_tag="OO199_000420"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5534598.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO199_000421"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO199_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5534599.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OO199_000422"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OO199_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5534600.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OO199_000423"
     CDS             complement(50650..51828)
                     /locus_tag="OO199_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5534601.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO199_000424"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO199_000424"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5534602.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OO199_000425"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OO199_000425"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5534603.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OO199_000426"
     CDS             complement(55610..55888)
                     /locus_tag="OO199_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5534604.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO199_000427"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO199_000427"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5534605.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OO199_000428"
     CDS             complement(56470..57279)
                     /locus_tag="OO199_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5534606.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO199_000429"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO199_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5534607.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO199_000430"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO199_000430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5534608.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO199_000431"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO199_000431"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5534609.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OO199_000432"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OO199_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5534610.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OO199_000433"
     CDS             complement(60980..61870)
                     /locus_tag="OO199_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5534611.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OO199_000434"
     CDS             complement(61857..62381)
                     /locus_tag="OO199_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534612.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OO199_000435"
     CDS             complement(62378..62848)
                     /locus_tag="OO199_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534613.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OO199_000436"
     CDS             complement(62845..63393)
                     /locus_tag="OO199_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534614.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OO199_000437"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OO199_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OO199_000438"
     CDS             complement(64140..64862)
                     /locus_tag="OO199_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5534615.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="OO199_000439"
     CDS             complement(64922..66154)
                     /locus_tag="OO199_000439"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5534616.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="OO199_000440"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="OO199_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67198)
                     /locus_tag="OO199_000441"
     CDS             complement(66863..67198)
                     /locus_tag="OO199_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534617.1"
                     /translation="MRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNN
                     VSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNFEA
                     NTTFQITYL"
BASE COUNT        16776 a        18201 c        16708 g        15552 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gagacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcag
//
LOCUS       DAJTNV010000007        67101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNV010000007 DAJTNV010000000
VERSION     DAJTNV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO199_000442"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO199_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5534618.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO199_000443"
     CDS             complement(1151..1237)
                     /locus_tag="OO199_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5534619.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO199_000444"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO199_000444"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5534620.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO199_000445"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO199_000445"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5534621.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO199_000446"
     CDS             3586..3630
                     /locus_tag="OO199_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534622.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO199_000447"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO199_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5534623.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO199_000448"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO199_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5534624.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO199_000449"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO199_000449"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5534625.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO199_000450"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO199_000450"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5534626.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO199_000451"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO199_000451"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5534627.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO199_000452"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO199_000452"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5534628.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO199_000453"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO199_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5534629.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO199_000454"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO199_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5534630.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="OO199_000455"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="OO199_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5534631.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="OO199_000456"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="OO199_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5534632.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="OO199_000457"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="OO199_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5534633.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="OO199_000458"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="OO199_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5534634.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="OO199_000459"
     CDS             complement(16982..17746)
                     /locus_tag="OO199_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5534635.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO199_000460"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO199_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5534636.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="OO199_000461"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="OO199_000461"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5534637.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="OO199_000462"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="OO199_000462"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5534638.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSLAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="OO199_000463"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="OO199_000463"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5534639.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="OO199_000464"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="OO199_000464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5534640.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="OO199_000465"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="OO199_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5534641.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="OO199_000466"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="OO199_000466"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5534642.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO199_000467"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO199_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5534643.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="OO199_000468"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="OO199_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5534644.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="OO199_000469"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="OO199_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5534645.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="OO199_000470"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="OO199_000470"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5534646.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="OO199_000471"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="OO199_000471"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5534647.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="OO199_000472"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="OO199_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5534648.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="OO199_000473"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="OO199_000473"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5534649.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO199_000474"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO199_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5534650.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO199_000475"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO199_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5534651.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO199_000476"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO199_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5534652.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="OO199_000477"
     CDS             complement(40422..41816)
                     /locus_tag="OO199_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5534653.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO199_000478"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO199_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5534654.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="OO199_000479"
     CDS             complement(42159..43445)
                     /locus_tag="OO199_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5534655.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="OO199_000480"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="OO199_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="OO199_000481"
     CDS             complement(44452..45222)
                     /locus_tag="OO199_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5534656.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45694..47208
                     /gene="caiT"
                     /locus_tag="OO199_000482"
     CDS             45694..47208
                     /gene="caiT"
                     /locus_tag="OO199_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5534657.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47239..48381
                     /gene="caiA"
                     /locus_tag="OO199_000483"
     CDS             47239..48381
                     /gene="caiA"
                     /locus_tag="OO199_000483"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5534658.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRRTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48510..49727
                     /gene="caiB"
                     /locus_tag="OO199_000484"
     CDS             48510..49727
                     /gene="caiB"
                     /locus_tag="OO199_000484"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5534659.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49801..51354
                     /gene="caiC"
                     /locus_tag="OO199_000485"
     CDS             49801..51354
                     /gene="caiC"
                     /locus_tag="OO199_000485"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5534660.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51463..52248
                     /gene="caiD"
                     /locus_tag="OO199_000486"
     CDS             51463..52248
                     /gene="caiD"
                     /locus_tag="OO199_000486"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5534661.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52254..52844
                     /gene="caiE"
                     /locus_tag="OO199_000487"
     CDS             52254..52844
                     /gene="caiE"
                     /locus_tag="OO199_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5534662.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52930..53325)
                     /gene="caiF"
                     /locus_tag="OO199_000488"
     CDS             complement(52930..53325)
                     /gene="caiF"
                     /locus_tag="OO199_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5534663.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53587..56808)
                     /gene="carB"
                     /locus_tag="OO199_000489"
     CDS             complement(53587..56808)
                     /gene="carB"
                     /locus_tag="OO199_000489"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5534664.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56826..57974)
                     /gene="carA"
                     /locus_tag="OO199_000490"
     CDS             complement(56826..57974)
                     /gene="carA"
                     /locus_tag="OO199_000490"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5534665.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58430..59251)
                     /gene="dapB"
                     /locus_tag="OO199_000491"
     CDS             complement(58430..59251)
                     /gene="dapB"
                     /locus_tag="OO199_000491"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5534666.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59418..60332)
                     /gene="rihC"
                     /locus_tag="OO199_000492"
     CDS             complement(59418..60332)
                     /gene="rihC"
                     /locus_tag="OO199_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5534667.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60398..61348)
                     /gene="ispH"
                     /locus_tag="OO199_000493"
                     /gene_synonym="lytB"
     CDS             complement(60398..61348)
                     /gene="ispH"
                     /locus_tag="OO199_000493"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5534668.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61350..61799)
                     /gene="fkpB"
                     /locus_tag="OO199_000494"
     CDS             complement(61350..61799)
                     /gene="fkpB"
                     /locus_tag="OO199_000494"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5534669.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61886..62380)
                     /gene="lspA"
                     /locus_tag="OO199_000495"
     CDS             complement(61886..62380)
                     /gene="lspA"
                     /locus_tag="OO199_000495"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5534670.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62380..65196)
                     /gene="ileS"
                     /locus_tag="OO199_000496"
     CDS             complement(62380..65196)
                     /gene="ileS"
                     /locus_tag="OO199_000496"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5534671.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65239..66180)
                     /gene="ribF"
                     /locus_tag="OO199_000497"
     CDS             complement(65239..66180)
                     /gene="ribF"
                     /locus_tag="OO199_000497"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5534672.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66188..66406)
                     /locus_tag="OO199_000498"
     CDS             complement(66188..66406)
                     /locus_tag="OO199_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5534673.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66509..66772
                     /gene="rpsT"
                     /locus_tag="OO199_000499"
     CDS             66509..66772
                     /gene="rpsT"
                     /locus_tag="OO199_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5534674.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18260 c        17267 g        15284 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatatttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaatgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg cctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaaccgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaagaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cctggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctagtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagttcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggaa ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggttgaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acactatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggta caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccag cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcccgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata
    45361 ttgttaagtt cctcaccaat attgaaaaca tggcgtagca aaaagaaatt tttaatattg
    45421 ttttatagat caccaatatt gaaagtcaga tgctattttt ttacccacaa aagaatataa
    45481 caaactgatt aataaccaaa attaacgaaa aacgccctct gttcactcga aacaaaaatg
    45541 tgacaccaat cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt
    45601 ccacgaggtt aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    45661 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    45721 atagaaccga aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca
    45781 gtcagagatc tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    45841 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    45901 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc
    45961 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    46021 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga
    46081 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    46141 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    46201 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggt
    46261 accatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    46321 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    46381 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    46441 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    46501 ctgggttggg tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    46561 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    46621 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc
    46681 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    46741 ggcatggtga tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac
    46801 actctgctgt tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt
    46861 gtggcgcgcg ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    46921 ggcttcttca tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc
    46981 ctggcgatgt ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt
    47041 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    47101 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    47161 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    47221 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    47281 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    47341 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    47401 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    47461 atggagctgg gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc
    47521 aataccttcc tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgccgc
    47581 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    47641 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    47701 tttattacca gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    47761 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    47821 accaaacttg agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat
    47881 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    47941 gaagagttcg accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc
    48001 gcctttgaag atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt
    48061 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    48121 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    48181 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag
    48241 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg
    48301 cgtgtagacc gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg
    48361 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc
    48421 aggcctacgg gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat
    48481 gcgctacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac
    48541 cgctggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac
    48601 aaatgttcgc agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca
    48661 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    48721 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    48781 tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    48841 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    48901 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    48961 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    49021 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    49081 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    49141 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    49201 cctactacgc cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg
    49261 tgggcattac ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta
    49321 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    49381 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    49441 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    49501 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    49561 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    49621 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    49681 acattcagga gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca
    49741 gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca
    49801 atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac
    49861 ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat
    49921 cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc
    49981 aaaggcaaca aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc
    50041 gggctggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa
    50101 agcgagtgga tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat
    50161 cctatgtatc aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca
    50221 gatgtggcac ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa
    50281 cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc
    50341 acctccggca ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc
    50401 gctggatatt actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta
    50461 atgcctgcgt ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg
    50521 gccacctttg tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag
    50581 taccgcgcca ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg
    50641 ccttcagcga acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg
    50701 gagcaggaaa aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg
    50761 atgacggaaa ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg
    50821 tcgattggtc gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg
    50881 ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc
    50941 aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta
    51001 cataccggcg ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc
    51061 tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc
    51121 gccacccatc cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat
    51181 gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt
    51241 tttcgcttct gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga
    51301 aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa
    51361 cgtcagggaa ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca
    51421 gggatgttgt cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat
    51481 ctgacccgca atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt
    51541 gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa
    51601 ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta
    51661 aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga
    51721 ttaaccgaaa ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt
    51781 ggcggcggtt tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc
    51841 ttcgccctgc cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg
    51901 ccgaagatcc tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc
    51961 gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg
    52021 gataacgccc gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg
    52081 ctgaaagaga tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt
    52141 cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg
    52201 ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct
    52261 attacgcctt tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg
    52321 ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac
    52381 tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca
    52441 ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg
    52501 gagcgatcct gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa
    52561 ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag
    52621 caggctttag tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca
    52681 gtgttagtga cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg
    52741 tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc
    52801 gcccccgttt gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac
    52861 ctggttgctg gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac
    52921 gagataaaat taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt
    52981 aagttccgtg agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc
    53041 ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag
    53101 tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct
    53161 tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat
    53221 atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc
    53281 ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc
    53341 acaaattaaa agtctggcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat
    53401 tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta
    53461 tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct
    53521 catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccacgac
    53581 acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc
    53641 ggcattcagc gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt
    53701 atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt
    53761 ggtgttgatg atgtaggtat attcgccatt cttgatacgg tcctgaatgt gcggacggcc
    53821 ttcatgcacc ttgtttacca gacgcggatt gatacccgct tcgcccagca caatcgccgt
    53881 gccgtgggtc gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac
    53941 gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga
    54001 gttgctgccc agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac
    54061 ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcctggga atttattgaa
    54121 cggcagcacc acttctttca ccgagtagta cggcggaata acttctttgg ttacgccctg
    54181 ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt
    54241 ggctttggag acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac
    54301 ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg cgcacctgca attcgaaggc
    54361 cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg
    54421 cagcgaacat gcggagtcac cggagtgcac acccgcctgc tcgatgtgct ccatgatgcc
    54481 gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc
    54541 aaggaaatga tccagcaaca ctggcgcatc gttagacacg ctaaccgccg tctggaagta
    54601 gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga
    54661 cggacgcacc accagcgggt agccaatctc ttttgccttc tcaactgcca tttcaatagc
    54721 agtaacggtg gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg
    54781 ttcacggtct tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc
    54841 ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc
    54901 tttcggcttc tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta
    54961 gaggcgatcg gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat
    55021 ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc
    55081 gataccctga ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt
    55141 agacggattc gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc
    55201 aaactctgcc gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg
    55261 cagcttacgg atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa
    55321 gcctttgcgt ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc
    55381 cactttctct tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt
    55441 agtcaggttg aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca
    55501 gatacgctct gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc
    55561 atccaggctc actttcgggt cgaagccagt tgcgcccact tccaggccgc gcagcgcttt
    55621 ttgcagggat tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg
    55681 agtggtcaga cggtcgttag caccggcgaa tttttcgaag ttgaagcgag gaattttagt
    55741 aaccacatag tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt
    55801 catcagttcg tctagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa
    55861 accggtcgct ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac
    55921 aatcaggcga ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac
    55981 gccgatttca cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcggt
    56041 cagcgtttgg gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa
    56101 gttttcgata gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc
    56161 gtactctttc cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc
    56221 cagaccgcgg gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct
    56281 accgcccatg gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaacagc
    56341 cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt
    56401 catcgctacg tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc
    56461 aatcatggtg acaccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt
    56521 cagcgccgtc tgaccgccca tggttggcag caccgcgtcc gggcgctctt tttcaataat
    56581 cttgcgtacc acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc
    56641 ggtcatgatg gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag
    56701 ggctttacat gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg
    56761 gcccgcaccc agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact
    56821 cctgattact tagcggtttt acggtactgc tcaattaact tgataaagtg gtcgaacaac
    56881 ggcgcggcgt cgtgtggacc agggctggct tcagggtgac cctggaagct gaacgccggc
    56941 ttatcgatgc gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc
    57001 aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc
    57061 accacgtttt tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt
    57121 ttgacagtct tcgcaccgct cgccagcgcc agcagctgat gaccgagaca gatgccgaat
    57181 accggaatat cggtttcgag gaacttctgg atggcggtaa tggcgtaatc gcacggggcc
    57241 gggtcgccag gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc
    57301 gcagaagttt gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg
    57361 atgttgcgct tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt
    57421 ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct
    57481 gcggtggtca cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt
    57541 tctaacgcca gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct
    57601 ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta
    57661 tggcgcttca ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc
    57721 aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta
    57781 gtgccgacat tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga
    57841 tcagtgagga tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc
    57901 gaacctgttg cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc
    57961 gctgacttaa tcaaaacacc ctccagagaa tactcactca ctttatttgc atattaattc
    58021 acagtgatga cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc
    58081 attctggaga tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt
    58141 ggctcatgca tgttttacag gcattaagcg acaaaaacat catctaacct gttctggaaa
    58201 acgcttgcgc agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat
    58261 ggtcaaaatt tacattaaaa caacaaaaat agaagacgaa acatgtaatt tttggcagtt
    58321 atagaaaatt taaaagcata acaccaaaaa taaaagggct aaaaattaaa gccctttata
    58381 attaataaat taaatgtgtt acttttgcat cataataaac attttgtggt tacaaattat
    58441 tgagatcaag cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag
    58501 ccgatcttac cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca
    58561 gacgctcgcc aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca
    58621 cggtagcaaa accaatggta cctggcacac gttcaccggt atggccttca cgactgtaga
    58681 ccgcgcaatc tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg
    58741 cggtgcctga cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg
    58801 tgtagtcacc catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa
    58861 cgctaaaatt ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt
    58921 taccggcttc gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tgccgacaaa
    58981 aagcgagatg gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat
    59041 cttttatcgc atcaagactg ctttgcacgg taacgcctgt tttccctgca ccagccagct
    59101 caccggcgtc gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg cccagctgca
    59161 cgccttctaa tgccagtgcc gcctgaatca actggcggcc catacgcccc ccggctcccg
    59221 cgatggcaac gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga
    59281 ccgttttcag agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa
    59341 attaatgata aacagggagg gatatcagtg aaaggcatga gtcgatgaat gactgcatgc
    59401 cgataacatg tgacaggtta cggcaccaga gccagcacct cagccaccca ctgttggaag
    59461 ccttttacat tcagatccaa tgccacctgt acattggctg gtttgcccag gcaaccgtcg
    59521 atatcaacca ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc gacaaaacac
    59581 ggcttgaggg tgaataagtc cgggcgcacc agccaggcga tggcgcagag atcgtgcatt
    59641 cgcagaccgc tttgcatact gccgctacgg tagtggctaa acagggcgtg gagcattttt
    59701 ccggtgcgat tcaactccgg taaagtagcg agatagtcag gggttaatat tgcctgattg
    59761 gtgacatcca gaccgcacat gacaatttcg atgccgctac ggaagacaca ggcagccgct
    59821 tctggatcgg cagcgatatt aaactcggca tttggtgtac agttgccgcg accagcagaa
    59881 ccgcccatga tcaccagacg acgaatatac ggtttacatt ccgggcattg taaaagtaac
    59941 agcgcaatat tggttaacgg gccgatggcc accagggtaa cgggctctgg tgcacgcatc
    60001 agggcatccc gaatcgccag aaacgccggt atcccgagtg gctttcggtt gtgctcaaca
    60061 aagtcgtagc cagccattcc cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt
    60121 accagcggca cagcggctcc ctgggcgagc ggaatctccg cattccagaa atgcagcagt
    60181 tgcagggcat tgcgggtagt tttctcaacc gagacattac ccgcgacggt ggtcatcagt
    60241 tgcaggtcga gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg
    60301 gggtcagtat cgaggaagat aggtaaacgc atgttttctt cataaaaaat gccggtaaca
    60361 agaccggcat tttcgcataa cttaggctgc taatgactta atcgacttca cgaatatcga
    60421 cacgcagctc tttcggcact tcgaaaacaa tattttcttc acggccttcc agcggaatgg
    60481 cttcaccacc acccaactgc tgcaaacgtg ccaccacatt ctgcaccaga atatccggag
    60541 ccgatgcgcc cgcagtcacg ccgacgcatt taacctcttt cacccactct tcctggatat
    60601 ctttcgcatc gtcaatcaaa aacgcgtgtt tgcccatacg ctgggccagc tccgccagac
    60661 ggttggagtt ggaggagttt ttcgaaccga ccaccaacac aacttccgcc tgttctgcca
    60721 gggcgcgtac cgcttcctga cggttagtcg tggcgtagca gatgtcatct ttgcgcggac
    60781 cgacaatttt cgggaagcgt ttacgcagcg cgtcgatcac atcagacgtg tcatccaccg
    60841 acagcgtggt ttgggtcata aaggagagct tctcttcgtt tttgaccgtc agtttccaca
    60901 catcgtctgg cgattcgacc agatacattc ccccttcggg gttgctgtac tgccccatcg
    60961 tcccttccac ttccgggtgc ccggcgtggc cgatgagaat agattcttcg ccacggcgac
    61021 tggcgcgggc aacttccata tgcactttgg tcaccagcgg acaggtggca tcaaacaccg
    61081 tcaaatcgcg gcttttcgct tcgttacgaa ccgcctgaga aacaccgtgt gcggagaaaa
    61141 tcaggatcgc gccgtccggc acttcgctaa tctgctcaat aaagatagcc ccacgctcgc
    61201 gcaagctatc gaccacgtag cggttatgca ccacttcgtg acggacatat atcggtgcgc
    61261 cgtaaatggc cagcgcgttt tcaacaatgc tgatagcgcg gtctaccccg gcacaaaaac
    61321 cacgcgggtt ggccaacagg atctgcatgt tacgcctcca gtgccggatc gatttccagc
    61381 acttcaatat caaaatgaac ggtctgcccg gccagcggat ggttgaaatc aacggtaatg
    61441 gagtcgccgt taatttcgcg gatcacacca ggcatctcac tgccatccat tgcggtaaaa
    61501 agcatgattg cgccaatttc tggctcgcct gcatccataa attcacggcg ggagaagtac
    61561 tgaatcaggt ccggtgacgg cacgccaaac gccgcatctg gctccagcga gaaggtggtt
    61621 ttgtcgccca ctttcagccc cagcaggtgt tgctccagcc cttcagaaag agaagcatcg
    61681 cccaggcgga acagcgccgg tttaccgttg ttgcgagtag actcggcggt ggtgccatcg
    61741 tcgagtttta gcgtgaagtg caccaggacg gcgctattgc tctgtacaga ttcagacatg
    61801 caggtttgct cttatttttg aggatttttg ccggatgcgg cctacgattt cgtagcaatc
    61861 tgtaggccgc atccggcagg gtttattatt gttttttcgc tttggaaggt aaaaaccctt
    61921 ccagcacgat cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga
    61981 agtgccagtc gccgacgtag aagtcgatca tatcgacaac gaagccgtgc cacaggcggt
    62041 cgaacaggtt gcccagcgcg ccgccaataa tcagcgcgta agcgatattg tttagcttct
    62101 gcgtggcctt cgagcgatac atcatcactg ccaggagcac gctaatacca atcgcaatac
    62161 cggcaaagaa ccaacgctgc cagccgccgc tatcggcaag gaaactaaac gccgcgccat
    62221 agttacgcgc ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa
    62281 agttctggag gatcaggtat ttgctgccca gatcgataat cagcacgact accaccagcc
    62341 acagccagcg tagccctgtt gaacagatcg attgactcat caggcaaact tacgtttttc
    62401 accgtcaccg gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt
    62461 gccgacatcc tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg ctttactcaa
    62521 cgcgactttc agccctttga gtacttcgct ctgctgagca tcagcaggtg cgtcgttata
    62581 gtctgcaacg gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc ccagcgcggt
    62641 cagtttcgcc gccagttccg gttctgcata caaggttact gccgcttcca gcgagccacc
    62701 cactttcttg tcggcacgcg cttgctcaat gactttgttc acttcgccac gcactttcaa
    62761 cagctcgtcc cagaacgcat cgttcattgc ttcactgtct gccagaccaa acaggccttc
    62821 gtaccactca ccggtgaaga cgtatttttc acgttcgccc ggcagatagc cccacacttc
    62881 atcagcggtg aaggagagga ttggtgccat ccagcgcacc agcgcttcag cgatgtgata
    62941 cagcgcagtc tggcagctac gacgcgccac gctatccgct ttggcggtgt actgacggtc
    63001 tttgatgatg tcgaggtaga aggaacccat ctcaacggag cagaagcgca tcagacgctg
    63061 taccacttcg tggaaatcgt atgcttcgta cgccttgagg atgtcttcct gtgccgcttt
    63121 cgcacaacct acggcccagc gatccagtac caccatctct tccggtttca ccatatcttt
    63181 tgctggatca aaaccattca ggtttgccag caggaagcgc gcggtgttac ggatacgacg
    63241 atagctatcg gcagcacgtt tcaggatctc gtcagaaacg gccatttcac cggtgtagtc
    63301 ggttgatgcc acccacagac gcagaatatc cgcgcccagt ttgttcatca catcctgcgg
    63361 cgaaacggtg ttgccgatgg atttagacat cttgcgaccc tgaccatcca cggtaaagcc
    63421 gtgggtcagt acctgacgat acggcgcttt gcctttcatc gcggtggaga tcattaggga
    63481 agacatgaac caaccacggt gctggtcaga accttccaga tacatgtccg ctgcgtgacc
    63541 ggcaaattcc ggacgcacgt caacaacaga agagtgggta gatccggagt caaaccatac
    63601 gtcgagagta tccggcactt tcacatactg gtcagcttcg tcgccgagga tctctttcgc
    63661 atcgagatcc caccacgcct ggatgccgtc aacttcaacg cgtttagcca cttcttccat
    63721 cagttcgagg gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg
    63781 tacgccccag gtgcgctgac gggagataca ccagtcagga cggttagcaa ccatcgactc
    63841 gatacgcgcc tggccccagt ccgggagcca ctgcacgcct ttgatctctt tcagtgactg
    63901 cgcacgcaga cctttctgat ccatgctgac gaaccactgc ggtgtcgcgc ggaagatgat
    63961 cggcgttttg tgacgccagc agcacggata gctgtgctgc attttctcaa cgtgcagcag
    64021 cgcgcctttt tcctgcagca gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc
    64081 atccagcgtc ggataagtgc ccggcagata agtgccgtcc gggccaaccg ggttagcggt
    64141 ttccaggccg tatttctgac cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg
    64201 aacggcaccg gtaccggcat ccagggtaac gtgatcgccg aggattgccg gaacgtcgaa
    64261 gcccataaac ggatgggtaa agcgcagcag ctcaagctct gcacctttta ccgtgccgag
    64321 aatggtgtaa tcggtcacgc cgatacgctg cattacgctt tcaaccagat ctttcgccag
    64381 aatcacggcc tgaccgtcga tctgcaccag cgcatagtcg aaatcaggtg caatagagat
    64441 tgcgcggttc gcaggcagag tccacggcgt agtggtccag atcaccagcg agattgggcc
    64501 gttaacgttg cttacgccaa atttcgcttt cagcgcatcc tgatcgaccg cctggaaagc
    64561 gacgtcaatg gacggagaag ttttgtcgta atactcaact tccgcttctg ccagtgcaga
    64621 acggcagtct acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac cattgccgat
    64681 gattttgccc agcgcgcgga tgatgttggc ttcagttttg aagtccatag tcagatacgg
    64741 gtgcgaccag tcgcccagca cgcccagacg gataaagtct ttgcgttgac cgtcaacctg
    64801 ggtcgccgcg tattcgcggc acttggcgcg gaactcggcg gcggtgaatt tctcacccgg
    64861 cttaccgtat tcttgctcta ctttcagctc gatcggcaga ccgtggcagt cccagccagg
    64921 cacatacggc gagtcataac cggaaagccc tttggacttc acgataatgt ctttcagaat
    64981 cttgttaacc gagtgaccaa tatgaatgct gccattcgca taaggagggc catcatgcag
    65041 aatgaaggtt tttttgcctt ttttagccgc acggatgatg ccgtacagat catcatcagt
    65101 ccaacgcgcc agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt
    65161 ttccggcaaa ttcagggttg atttatagtc actcatcaga ttctcggttc cgtgtttcgg
    65221 tttgattaca taacaggctt aagccggttt tgttagccca aaaaattcgc gggcggttaa
    65281 ttcatcacgc gcaatctgcg ctttcagttc gtccagcgac gcaaatcgct gctcattgcg
    65341 tattttttta cgcagcacta cttgtatatg gcgaccgtaa aggtccattg caacatctaa
    65401 caaatgcact tccagctgct ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc
    65461 cacgccgggt aacggctttt caccgaggcc cagcacttct accgcataaa cccctttcac
    65521 cggggaaacc tgacggcgta gcggtacatt cgccgtcggg aaacctatag tgcgccctaa
    65581 ttcatcaccg tggactacac gcccggagat ggcaaacggg tgccccagta aactttctgc
    65641 cagagccaga ttgtcatcag ccagcgcctg acgcacggcg gtgctgctga tacgcacacc
    65701 accttcgcaa aaagtttgcg tactggtgat atcgaagccg tattccatgc cagctttctg
    65761 taataacaag aaatcgcctt cacgaccagc gccaaagcgg aaatcatcac ctacggcaag
    65821 aaattttacg cgcaaatgct tcaccaaaag atcgctgatg aaattttgcg cggttaacgc
    65881 cgcaaaacgc ctgtcgaaac gcacgcacag cacgtaatca acgccacact ctgcaaggta
    65941 acgcagtttt tcccgcagcc gggtcagtct tgccggggct ttatcggtag caaacagttc
    66001 cagtggttga ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc gcccttcttc
    66061 ctgcaagccc tgtaacagcg cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt
    66121 cagcacacac ccttcttgcg gggcctggct gagattatgt atgccgcgta tcagcttcat
    66181 gtctggctca aaacagtgaa aatcgtccga gtataccttg tacagcggtc aaggttaacc
    66241 ggcgattgag taccgaaatc agaaagaagt ggcttttcat tgccatggag caaatcacgg
    66301 gaagaaactg accgcctgct gcaattttta tcgcggaaaa gctgtattca caccccgcaa
    66361 gctggtagaa tcctgcgcca tcactacgta acgagtgccg gcacattaac ggcgcttatt
    66421 tgcacaaatc cattgacaaa agaaggctaa aagggcatat tcctcggcct ttgaattgtc
    66481 catatagaac acatttggga gttggacctt ggctaatatc aaatcagcta agaagcgcgc
    66541 cattcagtct gaaaaggctc gtaagcacaa cgcaagccgt cgctctatga tgcgtacttt
    66601 catcaagaaa gtatacgcag ctatcgaagc tggcgacaaa gctgctgcac agaaagcatt
    66661 taacgaaatg caaccgatcg tggaccgtca ggctgctaaa ggtctgatcc acaaaaacaa
    66721 agctgcacgt cataaggcta acctgactgc acagatcaac aaactggctt aatcgccaat
    66781 ttgctgaagc tttgtgaaaa agcccgcgca agcgggtttt tttatgcctg ctgcttttgt
    66841 gatgcgtgag agaacacact gccggttgcc agatagtcaa aagagacatg ccgttgtact
    66901 atctgaactc ttcgtggctt aagaatcgta gaaccatcgg ctgatacatt ctgaacaaga
    66961 atatctactc aatctccaga ctcccccaca aagaatatgg atattgtgat acacattgag
    67021 gtattaaact taagaccact ctatttttga attaaattcc tatatacttt aaccattcat
    67081 aaatatctcc tcattagacc t
//
LOCUS       DAJTNV010000008        65614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNV010000008 DAJTNV010000000
VERSION     DAJTNV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO199_000500"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO199_000500"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5534675.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO199_000501"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO199_000501"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5534676.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO199_000502"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO199_000502"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5534677.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO199_000503"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO199_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5534678.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO199_000504"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO199_000504"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5534679.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO199_000505"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO199_000505"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5534680.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO199_000506"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO199_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5534681.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO199_000507"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO199_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5534682.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO199_000508"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO199_000508"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5534683.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO199_000509"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO199_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5534684.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO199_000510"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO199_000510"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5534685.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO199_000511"
     CDS             complement(15665..16702)
                     /locus_tag="OO199_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5534686.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO199_000512"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO199_000512"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5534687.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO199_000513"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO199_000513"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5534688.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO199_000514"
     CDS             19444..19884
                     /locus_tag="OO199_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5534689.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO199_000515"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO199_000515"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5534690.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO199_000516"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO199_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO199_000517"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO199_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5534691.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO199_000518"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO199_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5534692.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO199_000519"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO199_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5534693.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO199_000520"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO199_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5534694.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO199_000521"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO199_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5534695.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO199_000522"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO199_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5534696.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO199_000523"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO199_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5534697.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO199_000524"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO199_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5534698.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO199_000525"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO199_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5534699.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO199_000526"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO199_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5534700.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO199_000527"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO199_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5534701.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO199_000528"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO199_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5534702.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO199_000529"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO199_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5534703.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO199_000530"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO199_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5534704.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OO199_000531"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OO199_000531"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5534705.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OO199_000532"
     CDS             37412..37681
                     /locus_tag="OO199_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5534706.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OO199_000533"
     CDS             complement(37684..38043)
                     /locus_tag="OO199_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5534707.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OO199_000534"
     CDS             38184..38810
                     /locus_tag="OO199_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5534708.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OO199_000535"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OO199_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5534709.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO199_000536"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO199_000536"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5534710.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OO199_000537"
     CDS             41650..42315
                     /locus_tag="OO199_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5534711.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OO199_000538"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OO199_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5534712.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OO199_000539"
     CDS             complement(42949..44166)
                     /locus_tag="OO199_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5534713.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OO199_000540"
     CDS             44300..45349
                     /locus_tag="OO199_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5534714.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OO199_000541"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OO199_000541"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5534715.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OO199_000542"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OO199_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5534716.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OO199_000543"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OO199_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5534717.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OO199_000544"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OO199_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5534718.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OO199_000545"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OO199_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5534719.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OO199_000546"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OO199_000546"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5534720.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OO199_000547"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OO199_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5534721.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OO199_000548"
     CDS             complement(51544..51903)
                     /locus_tag="OO199_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5534722.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OO199_000549"
     CDS             complement(51900..52175)
                     /locus_tag="OO199_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5534723.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OO199_000550"
     CDS             complement(52234..53358)
                     /locus_tag="OO199_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5534724.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO199_000551"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO199_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5534725.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OO199_000552"
     CDS             complement(56090..57157)
                     /locus_tag="OO199_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5534726.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OO199_000553"
     CDS             complement(57523..59145)
                     /locus_tag="OO199_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5534727.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="OO199_000554"
     CDS             complement(59407..60996)
                     /locus_tag="OO199_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5534728.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="OO199_000555"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="OO199_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="OO199_000556"
     CDS             complement(62499..62975)
                     /locus_tag="OO199_000556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534729.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="OO199_000557"
     CDS             complement(62968..63732)
                     /locus_tag="OO199_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5534730.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="OO199_000558"
     CDS             63978..64739
                     /locus_tag="OO199_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5534731.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="OO199_000559"
     CDS             complement(64846..65346)
                     /locus_tag="OO199_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534732.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15939 a        17620 c        16160 g        15895 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatagcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctaaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DAJTNV010000009        65461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNV010000009 DAJTNV010000000
VERSION     DAJTNV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            121..564
                     /locus_tag="OO199_000560"
     CDS             121..564
                     /locus_tag="OO199_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5534733.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO199_000561"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO199_000561"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5534734.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO199_000562"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO199_000562"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5534735.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO199_000563"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO199_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5534736.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO199_000564"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO199_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5534737.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO199_000565"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO199_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5534738.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO199_000566"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO199_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5534739.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO199_000567"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO199_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5534740.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO199_000568"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO199_000568"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5534741.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO199_000569"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO199_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5534742.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO199_000570"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO199_000570"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5534743.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO199_000571"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO199_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5534744.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO199_000572"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO199_000572"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5534745.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO199_000573"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO199_000573"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5534746.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO199_000574"
     CDS             13924..14304
                     /locus_tag="OO199_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5534747.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO199_000575"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO199_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5534748.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO199_000576"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO199_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5534749.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO199_000577"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO199_000577"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5534750.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO199_000578"
     tRNA            complement(17405..17480)
                     /locus_tag="OO199_000578"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO199_000579"
     tRNA            complement(17484..17559)
                     /locus_tag="OO199_000579"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO199_000580"
     tRNA            complement(17611..17686)
                     /locus_tag="OO199_000580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO199_000581"
     tRNA            complement(17689..17764)
                     /locus_tag="OO199_000581"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17901..17976)
                     /locus_tag="OO199_000582"
     tRNA            complement(17901..17976)
                     /locus_tag="OO199_000582"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17941..17943),aa:Lys,seq:ttt)
     gene            complement(18141..18932)
                     /gene="cpoB"
                     /locus_tag="OO199_000583"
     CDS             complement(18141..18932)
                     /gene="cpoB"
                     /locus_tag="OO199_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5534751.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18942..19463)
                     /gene="pal"
                     /locus_tag="OO199_000584"
     CDS             complement(18942..19463)
                     /gene="pal"
                     /locus_tag="OO199_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5534752.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19498..20790)
                     /gene="tolB"
                     /locus_tag="OO199_000585"
     CDS             complement(19498..20790)
                     /gene="tolB"
                     /locus_tag="OO199_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5534753.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20923..22164)
                     /gene="tolA"
                     /locus_tag="OO199_000586"
     CDS             complement(20923..22164)
                     /gene="tolA"
                     /locus_tag="OO199_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5534754.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22229..22657)
                     /gene="tolR"
                     /locus_tag="OO199_000587"
     CDS             complement(22229..22657)
                     /gene="tolR"
                     /locus_tag="OO199_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5534755.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22661..23353)
                     /gene="tolQ"
                     /locus_tag="OO199_000588"
     CDS             complement(22661..23353)
                     /gene="tolQ"
                     /locus_tag="OO199_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5534756.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23350..23754)
                     /gene="ybgC"
                     /locus_tag="OO199_000589"
     CDS             complement(23350..23754)
                     /gene="ybgC"
                     /locus_tag="OO199_000589"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5534757.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23904..24197)
                     /gene="ybgE"
                     /locus_tag="OO199_000590"
     CDS             complement(23904..24197)
                     /gene="ybgE"
                     /locus_tag="OO199_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5534758.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24197..24310)
                     /gene="cydX"
                     /locus_tag="OO199_000591"
     CDS             complement(24197..24310)
                     /gene="cydX"
                     /locus_tag="OO199_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5534759.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24325..25464)
                     /gene="cydB"
                     /locus_tag="OO199_000592"
     CDS             complement(24325..25464)
                     /gene="cydB"
                     /locus_tag="OO199_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5534760.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25480..27048)
                     /gene="cydA"
                     /locus_tag="OO199_000593"
     CDS             complement(25480..27048)
                     /gene="cydA"
                     /locus_tag="OO199_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5534761.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27897..30530)
                     /gene="mngB"
                     /locus_tag="OO199_000594"
                     /pseudo
     CDS             complement(27897..30530)
                     /gene="mngB"
                     /locus_tag="OO199_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30548..32464)
                     /gene="mngA"
                     /locus_tag="OO199_000595"
     CDS             complement(30548..32464)
                     /gene="mngA"
                     /locus_tag="OO199_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5534762.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32634..33356
                     /gene="mngR"
                     /locus_tag="OO199_000596"
     CDS             32634..33356
                     /gene="mngR"
                     /locus_tag="OO199_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5534763.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33459..34328)
                     /gene="sucD"
                     /locus_tag="OO199_000597"
     CDS             complement(33459..34328)
                     /gene="sucD"
                     /locus_tag="OO199_000597"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5534764.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     QDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34328..35494)
                     /gene="sucC"
                     /locus_tag="OO199_000598"
     CDS             complement(34328..35494)
                     /gene="sucC"
                     /locus_tag="OO199_000598"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5534765.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35588..36805)
                     /gene="odhB"
                     /locus_tag="OO199_000599"
     CDS             complement(35588..36805)
                     /gene="odhB"
                     /locus_tag="OO199_000599"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5534766.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGSRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36820..39621)
                     /gene="sucA"
                     /locus_tag="OO199_000600"
     CDS             complement(36820..39621)
                     /gene="sucA"
                     /locus_tag="OO199_000600"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5534767.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39922..40638)
                     /gene="sdhB"
                     /locus_tag="OO199_000601"
     CDS             complement(39922..40638)
                     /gene="sdhB"
                     /locus_tag="OO199_000601"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5534768.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40654..42420)
                     /gene="sdhA"
                     /locus_tag="OO199_000602"
     CDS             complement(40654..42420)
                     /gene="sdhA"
                     /locus_tag="OO199_000602"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5534769.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42420..42767)
                     /gene="sdhD"
                     /locus_tag="OO199_000603"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42420..42767)
                     /gene="sdhD"
                     /locus_tag="OO199_000603"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5534770.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42761..43165)
                     /gene="sdhC"
                     /locus_tag="OO199_000604"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42761..43165)
                     /gene="sdhC"
                     /locus_tag="OO199_000604"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5534771.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43546..43653
                     /locus_tag="OO199_000605"
     CDS             43546..43653
                     /locus_tag="OO199_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534772.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43859..45142
                     /gene="gltA"
                     /locus_tag="OO199_000606"
     CDS             43859..45142
                     /gene="gltA"
                     /locus_tag="OO199_000606"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5534773.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45533..46099
                     /locus_tag="OO199_000607"
     CDS             45533..46099
                     /locus_tag="OO199_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5534774.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46150..48609
                     /locus_tag="OO199_000608"
     CDS             46150..48609
                     /locus_tag="OO199_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5534775.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48624..49355
                     /locus_tag="OO199_000609"
     CDS             48624..49355
                     /locus_tag="OO199_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5534776.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49352..50428
                     /locus_tag="OO199_000610"
                     /pseudo
     CDS             49352..50428
                     /locus_tag="OO199_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50580..51626
                     /locus_tag="OO199_000611"
     CDS             50580..51626
                     /locus_tag="OO199_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5534777.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51623..52414)
                     /gene="nei"
                     /locus_tag="OO199_000612"
     CDS             complement(51623..52414)
                     /gene="nei"
                     /locus_tag="OO199_000612"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5534778.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52450..53184)
                     /gene="pxpA"
                     /locus_tag="OO199_000613"
     CDS             complement(52450..53184)
                     /gene="pxpA"
                     /locus_tag="OO199_000613"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5534779.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53174..54106)
                     /gene="pxpC"
                     /locus_tag="OO199_000614"
                     /pseudo
     CDS             complement(53174..54106)
                     /gene="pxpC"
                     /locus_tag="OO199_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54100..54756)
                     /gene="pxpB"
                     /locus_tag="OO199_000615"
     CDS             complement(54100..54756)
                     /gene="pxpB"
                     /locus_tag="OO199_000615"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5534780.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54779..55522)
                     /gene="ybgI"
                     /locus_tag="OO199_000616"
     CDS             complement(54779..55522)
                     /gene="ybgI"
                     /locus_tag="OO199_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5534781.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55793..57274
                     /gene="dtpD"
                     /locus_tag="OO199_000617"
     CDS             55793..57274
                     /gene="dtpD"
                     /locus_tag="OO199_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5534782.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57424..58842)
                     /gene="phrB"
                     /locus_tag="OO199_000618"
     CDS             complement(57424..58842)
                     /gene="phrB"
                     /locus_tag="OO199_000618"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5534783.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58839..59348)
                     /locus_tag="OO199_000619"
     CDS             complement(58839..59348)
                     /locus_tag="OO199_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5534784.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59477..59764
                     /locus_tag="OO199_000620"
     CDS             59477..59764
                     /locus_tag="OO199_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5534785.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59819..60199)
                     /locus_tag="OO199_000621"
     CDS             complement(59819..60199)
                     /locus_tag="OO199_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534786.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60202..64866)
                     /locus_tag="OO199_000622"
                     /pseudo
     CDS             complement(60202..64866)
                     /locus_tag="OO199_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(64885..65088)
                     /locus_tag="OO199_000623"
                     /pseudo
     CDS             complement(64885..65088)
                     /locus_tag="OO199_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15865 a        17730 c        15978 g        15888 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatctcg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag ctggcagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac
    17821 tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acgttttact
    17881 accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    17941 aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt
    18001 ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa
    18061 tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac
    18121 ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca
    18181 tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca
    18241 cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc
    18301 ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg
    18361 tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt
    18421 ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc
    18481 gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc
    18541 gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg atcgctgctg
    18601 gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt
    18661 tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa
    18721 tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga
    18781 gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta
    18841 ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga
    18901 ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca
    18961 cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt ttctttaccg
    19021 taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg
    19081 gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg
    19141 tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc
    19201 agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg
    19261 ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg
    19321 ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc gtcattgctg
    19381 gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct
    19441 ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta attattatca
    19501 cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt
    19561 gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta
    19621 gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag
    19681 aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga
    19741 gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc
    19801 ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg
    19861 accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt
    19921 attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta
    19981 caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa
    20041 tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag
    20101 cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc
    20161 gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt
    20221 gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac
    20281 aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc
    20341 atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg
    20401 agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga
    20461 accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca
    20521 tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa
    20581 cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg
    20641 tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac
    20701 accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa
    20761 accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca
    20821 cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca
    20881 accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa
    20941 tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag
    21001 tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc
    21061 cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga
    21121 cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat
    21181 atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt
    21241 ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat
    21301 atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc
    21361 tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt
    21421 atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt
    21481 cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc
    21541 agcagcttca gttgctgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc
    21601 tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt
    21661 ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg ctgcttcttc
    21721 cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa
    21781 ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc
    21841 tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc
    21901 agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct
    21961 ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga
    22021 cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag
    22081 cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc
    22141 gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct
    22201 ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt
    22261 tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt
    22321 ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg
    22381 ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact
    22441 gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg
    22501 attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca
    22561 taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg atttcggact
    22621 tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc
    22681 taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat
    22741 tcagttccag tttgtttacg cgctggttga gacggttata agccattacc gccggaattg
    22801 cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt
    22861 gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg
    22921 taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg
    22981 tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca
    23041 ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc
    23101 tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga
    23161 ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg
    23221 ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa
    23281 tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat
    23341 tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat
    23401 tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc
    23461 gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg
    23521 tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac
    23581 cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat
    23641 ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc
    23701 atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca
    23761 tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga
    23821 ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg agcggcccat
    23881 aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca
    23941 atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc
    24001 acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc
    24061 agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga
    24121 agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc
    24181 gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa
    24241 gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga
    24301 aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc
    24361 tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg
    24421 taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc
    24481 ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc
    24541 gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc
    24601 ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac ccagtgccgg
    24661 aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac
    24721 cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata
    24781 accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag
    24841 cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg
    24901 cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc
    24961 gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac
    25021 gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac
    25081 gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt
    25141 ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc
    25201 cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc
    25261 gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat
    25321 aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc
    25381 gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa
    25441 acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt
    25501 cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc
    25561 aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag
    25621 caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag
    25681 cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac
    25741 agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc
    25801 aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc
    25861 acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt
    25921 ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag
    25981 cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc
    26041 ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg
    26101 ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt
    26161 tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg
    26221 atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc
    26281 aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag
    26341 aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt
    26401 agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat
    26461 cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt
    26521 aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga
    26581 cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga
    26641 accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc
    26701 gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc
    26761 cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa
    26821 ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt
    26881 gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc
    26941 cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat
    27001 cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg
    27061 ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa
    27121 tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag
    27181 aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa
    27241 aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat
    27301 ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat
    27361 gcccatttcc aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg
    27421 tttcaacaag gtaactttag tgatatttat gtttaaaatg tgtgaattat aagactgaaa
    27481 ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct
    27541 taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc
    27601 ataaatctcc cccccgccga tattaacatt taattaactt ataattatat tataattaac
    27661 tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag
    27721 ataaacacaa agttacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat
    27781 attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca
    27841 aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag
    27901 gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa
    27961 ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc
    28021 tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt
    28081 aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg
    28141 ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc
    28201 gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga
    28261 gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc
    28321 agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaagcctt
    28381 aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt
    28441 ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg
    28501 ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct
    28561 ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa
    28621 ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga
    28681 aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta
    28741 tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct tgcgctgcgt
    28801 tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc
    28861 tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt
    28921 gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg
    28981 tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca
    29041 atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg
    29101 ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg
    29161 atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg
    29221 attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga
    29281 tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc
    29341 cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg
    29401 tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg
    29461 aagcgggtga cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg
    29521 gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg gtatccaaaa
    29581 cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt attttcaata
    29641 cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg cacgcgcata
    29701 tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg cgcttcgatc
    29761 ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata aatttcgcgc
    29821 agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg cccattcggc
    29881 agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact gtcgaggcgt
    29941 ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc caacggcatc
    30001 acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt tttatccgta
    30061 ccgtggcgct cagaacatcc acgccagaac atggtgcagg taatgccaaa tccattgtag
    30121 atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat tttcatcggc
    30181 tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat ggattccgca
    30241 gaaacaatcg tggtatcggt ctgggtatac taggggccga taatcaactt gccggcttct
    30301 accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc
    30361 gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag tcggcacagg
    30421 atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa ataccactcc
    30481 cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc
    30541 tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc
    30601 gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca ccaaaccagc
    30661 caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc gagaaaatgc
    30721 cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc gttaccatag
    30781 agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc gggatcgccc
    30841 cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca atttcaaact
    30901 ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc gaaaccattt
    30961 tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg cccaggcaga
    31021 atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata aaaccgagaa
    31081 tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg gtaagcgagt
    31141 tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc gctcccaatg
    31201 taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg ctaagacgca
    31261 agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg accgcgccga
    31321 gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc gctaacgccg
    31381 gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc aaaattccga
    31441 gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca ttaaacagat
    31501 cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc ccccccgcga
    31561 caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc tccgttttga
    31621 cgcttttcac cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca cgctcgaggg
    31681 taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca ggcacagaaa
    31741 gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc gcaaaaatac
    31801 aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg ttagcacctt
    31861 gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga tactccgcag
    31921 ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata gttggcgcgt
    31981 tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag agcaattcat
    32041 caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg cgcgtggtca
    32101 gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc gccagcaaca
    32161 ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc tcgctgagtg
    32221 tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc acagccaacc
    32281 cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc agaaattgct
    32341 cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga atggcctctt
    32401 cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga gtcgtcaggt
    32461 tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga caaaacaata
    32521 ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt attgtttaca
    32581 ggatgaattt atcgggcata atggctaaat tcgtattaat gagatgctaa ataatgggac
    32641 acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg cgtggcgagc
    32701 ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt ggcgtcagcc
    32761 gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc gaaagcattc
    32821 agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt cagttaacca
    32881 gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt ctgatatttg
    32941 aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg caggatcgtg
    33001 tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg gaagaaacct
    33061 ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat tcgaaatatc
    33121 actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa atcattcctc
    33181 tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag ccgattcttg
    33241 aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt
    33301 ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg cgataagcac
    33361 aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt
    33421 agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt
    33481 gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg
    33541 aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg gcccatacgt
    33601 ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt aacatgctct
    33661 ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc
    33721 gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt agagccaggg
    33781 atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc cgtggtctgt
    33841 ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg
    33901 tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac gcctgggcag
    33961 ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt cagcatatcc
    34021 tgcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc gatagcttcc
    34081 agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga agcgccagta
    34141 gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtgccgcct
    34201 ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg ttctgagtgg
    34261 aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt atcaattaaa
    34321 atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt
    34381 ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgctccgagt tcggcgttgt
    34441 taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc gcgccgatga
    34501 taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc agaacggctt
    34561 tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc
    34621 cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact atgtccatcg
    34681 tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt
    34741 agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca
    34801 tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt ttgccgtcga
    34861 ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc agcgccaggt
    34921 cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt
    34981 taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca
    35041 gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact ttttcgattt
    35101 ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg tctacaacgg
    35161 caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc tggttaaccg
    35221 gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag ttttctgcaa
    35281 aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac
    35341 cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa gcggcttctt
    35401 ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa
    35461 acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct tcagtaatcg
    35521 ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct
    35581 taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct ttgatcgtta
    35641 ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt
    35701 acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt
    35761 gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga
    35821 acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc
    35881 catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg
    35941 tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc gccatgctga
    36001 cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc acttccgggt
    36061 aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc
    36121 cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt
    36181 tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg
    36241 ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg
    36301 gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat
    36361 gtttttccac atcttcacga gtcagacgac tacccacacc ggtgccttta atggcgctgg
    36421 cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt
    36481 gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg
    36541 cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg
    36601 ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta
    36661 cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt
    36721 cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag
    36781 ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc
    36841 attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc
    36901 ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg
    36961 atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc
    37021 cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag
    37081 ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag
    37141 gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagctc
    37201 gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac
    37261 cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac gacgcatccc
    37321 gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gtacgcaaac
    37381 ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc
    37441 cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca
    37501 tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc
    37561 accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat aaccatattc
    37621 aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg cgccctgacc
    37681 gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc
    37741 gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc
    37801 ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc
    37861 accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac
    37921 tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca tctcaacttt
    37981 gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat
    38041 gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag
    38101 gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc
    38161 gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg gctgggttgc
    38221 gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt cgatgaagac
    38281 atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc
    38341 cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca
    38401 gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct ggttgttgat
    38461 aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc
    38521 ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt
    38581 gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga taacgaccgg
    38641 gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc
    38701 ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc cgaggtgttc
    38761 tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac
    38821 cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg tgccgctgtt
    38881 gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga
    38941 gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt cagcggcggt
    39001 cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat
    39061 acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact cggcaccaat
    39121 cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca tggtttcttt
    39181 gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg tcagatcgtg
    39241 gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg gatctagatt
    39301 cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt
    39361 cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg
    39421 gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa
    39481 ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta agaagtcttc
    39541 atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc
    39601 tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg
    39661 tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctacg
    39721 tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat
    39781 ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg ggtaatgact
    39841 tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg caacgcttgc
    39901 gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact tgatatggcc
    39961 gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg
    40021 acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc
    40081 acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc
    40141 gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata
    40201 cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt
    40261 ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat
    40321 gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt
    40381 gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat
    40441 gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg
    40501 atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac cttcttccgc
    40561 ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat
    40621 tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa
    40681 tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactcagg
    40741 cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct
    40801 atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc
    40861 catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc
    40921 atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc
    40981 catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt
    41041 acggatcgcc accggatctt caccgttacg attattgttc caccggttca ggcgatccag
    41101 agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg cgatagactc
    41161 ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc
    41221 cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagtcccgg
    41281 aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa ctttggtcgg
    41341 aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc
    41401 gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc
    41461 tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg
    41521 accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt
    41581 ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc
    41641 ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa
    41701 ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc
    41761 gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc cgccagtcgc
    41821 cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt
    41881 acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa
    41941 aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt
    42001 acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc
    42061 gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca ggcccatgtg
    42121 ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc
    42181 accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg
    42241 ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg
    42301 gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg
    42361 catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat
    42421 cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc
    42481 accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa cacctgccac
    42541 atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg
    42601 aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa
    42661 aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg
    42721 aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg
    42781 cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt
    42841 cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt
    42901 gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa
    42961 tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccactgca
    43021 ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg
    43081 ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat
    43141 ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca
    43201 gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcggg
    43261 tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa
    43321 cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggcag
    43381 tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct
    43441 gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca
    43501 atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg taaaagttat
    43561 gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca
    43621 ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc
    43681 gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc tgtacccagg
    43741 ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt
    43801 gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat
    43861 ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac tggatgtgct
    43921 gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt
    43981 cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga
    44041 tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc
    44101 taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta
    44161 tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct
    44221 gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta ttaccggcgc
    44281 gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc gtgaaattgc
    44341 cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat
    44401 tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat
    44461 gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga
    44521 ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac
    44581 cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg
    44641 gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc
    44701 cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct
    44761 gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga
    44821 aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat
    44881 ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa
    44941 cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac
    45001 cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag
    45061 ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgactt
    45121 taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgccgt
    45181 tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccac
    45241 tatcggacat tcatatattc taaaaaaaaa tacatcggta taaattataa acaaatagat
    45301 gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat atagcgcacc
    45361 cctataattc ccaatacttt attcatcaaa catttaattc ataccatcaa ttaattactt
    45421 ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg acaatcaaat
    45481 aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag ttatgtttaa
    45541 aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac ctgtttatgc
    45601 ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga
    45661 aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat
    45721 taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca actgtgatct
    45781 ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca cctttgatag
    45841 ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagccac
    45901 cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac
    45961 tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct ttgcctggat
    46021 ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga atgcaaccta
    46081 cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt cataaaatat
    46141 ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg cctggtcgtg
    46201 gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa atcaatgcgc
    46261 gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg tgagtatttt
    46321 gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa ctggcacaaa
    46381 aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt tggcttaaaa
    46441 cctgaagtcc gtcagtcgtt accattgata aatcagtgcg tcgattttag ctcccgacct
    46501 gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat tccgcaagcc
    46561 tggctggcgt ggcactcaga aaactggacc ccaccctcca catggaaaga aggtgtcgcc
    46621 ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga tggcagcagc
    46681 agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc
    46741 agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc aggcgaaata
    46801 tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac cctcggcgaa
    46861 acggatttta gtcccaatat tttcgacggt ttttcttata ccggcgcggc actggcaagt
    46921 gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag cggtattgca
    46981 cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc
    47041 ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac actggatgtc
    47101 aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc atcgacgccc
    47161 ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc acggccctcc
    47221 atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg
    47281 tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca ttctgccgca
    47341 atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc
    47401 agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt taattacagc
    47461 aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt ctccgatcgt
    47521 cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga tgcgcaggac
    47581 gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa cctcaatctt
    47641 tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc
    47701 acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc tacgtcattc
    47761 aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc gatttcgctc
    47821 cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca
    47881 caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga
    47941 ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt
    48001 tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc
    48061 agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc
    48121 tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag
    48181 ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac
    48241 cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa
    48301 aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt
    48361 tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga
    48421 gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat
    48481 gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag
    48541 cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct
    48601 tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg
    48661 ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg
    48721 ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg
    48781 cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc
    48841 cgccgataca acgtctggaa ccgaaagcaa caacacaggt acggatagta aagcaagcct
    48901 cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc
    48961 ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta
    49021 tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc
    49081 agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc
    49141 tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga
    49201 tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt
    49261 atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac
    49321 aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt
    49381 catctgtctg ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct actttggttc
    49441 atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc
    49501 caaactcggc gataagatct gggaatctga cgatattaaa ataagatctg ggaatctgac
    49561 gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaactttga aagcgaacat
    49621 gtctacgcat gggtaaaccc ctacccgggg gtgcaggatc gttattatca actcggagta
    49681 acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag
    49741 tgtatcgaca gtaagaacat tgacatttat acccctgatg ggatggcaga acaaaatttg
    49801 ctcgggtgat cccgctaata ttcacatgtc gcgaaccttt ctcgctcgca tgcggttata
    49861 cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt
    49921 cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata
    49981 tcatattact ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc
    50041 accggaaaca cagatgattg attttggtaa atttaatttg ctggatattc gtcgccacac
    50101 aatgtcgaaa acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg
    50161 atttaaagtc agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt
    50221 gttgattggt aatggtctga agctacgttt gctggatgaa aacgcctcgc cttatacctt
    50281 caataaatat gctgaatacg ccgatttcac cagcgacatg ttggtctacg aaaaaaccta
    50341 tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag gctgggccct tcgatacagt
    50401 ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag ccggaaaggg
    50461 catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta ttttttccta
    50521 gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg gagcgaagga
    50581 tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct attggtttcc
    50641 tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg atcatcttca
    50701 gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg caggccattc
    50761 ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg gccgccaact
    50821 ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc gtgggttggt
    50881 tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct tctccaggcg
    50941 gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc ctggtggcgt
    51001 ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga
    51061 tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg ccagtgagca
    51121 ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga tgcatgctgc
    51181 gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc
    51241 agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg gcaattggct
    51301 ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc ccgctaccgc
    51361 aaatcctgct gtcgattttt gctctgctgg ctatttgtgc aggtatggcg tggggactga
    51421 cccggtttat gcatattgat tttatgactg cctacctcgc caccagtccc ggcgggcttg
    51481 ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt atggcgatgc
    51541 aaaccctgcg cctgttcagt attttgctga cggggccagc cattgcgcgg tttatttcaa
    51601 cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg acaccagtaa aacgggcgcg
    51661 aggacaacgt ggttttctca atgatgccgc cacagcgttc gcacagttcg ccatcacgat
    51721 gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc tgcccccgcg
    51781 tagcgtaaga aaaccgagga atctccagta acgcgtgtgc gagtgcatcc agttgcgccg
    51841 cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg atctccaccc
    51901 gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct gcaaactgac
    51961 ggttacgaaa gcgcggcgac aataaccgtt cctttaccac ctccggcgtc agattcggat
    52021 ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggcctca
    52081 acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta
    52141 cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt
    52201 aaagctgatt atggctgtag agcgttaaac cgttgggaaa atgagttaac aacgccttac
    52261 cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa gtttttaact
    52321 gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat
    52381 ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat
    52441 taagcggggt tatgctgcga caacaatccc ctttttggca aatgtagctc gtaatcggcg
    52501 ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag accgtttgcg ccgcgaccgt
    52561 tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcca gcgtttgcgc
    52621 caacgcctgt tcttcgtttt caatcaacgc gcctggctga cttcgcggca ccagcgagcc
    52681 gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc catattgctt
    52741 gcctgcacga atcagctcgc ttcctgccag cccaacgaga accaatgccg gatcgcaagc
    52801 gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa
    52861 catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa tcgttgccag
    52921 cgcgccaatt tgatacagcg tctgggcgta aacggtttcc ggcggcagct gcatggcgct
    52981 gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt
    53041 tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg cctgcatgaa atccacaagc
    53101 aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag
    53161 atcggcgttc aggtcaattt tcattgtgca gccgccacgc taattgttca aaataacgtt
    53221 gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct
    53281 gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag gcaatacgtg
    53341 gataaccacc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg ttatgcggca
    53401 cctgcaccac gctcggtaac aaaccgtgag ataacagttc gcgatcggtg atgcgtttta
    53461 aaatttgccc ctgtaaacga tagcccattc ggttactttg cgagctaagc tgccagggcg
    53521 aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg
    53581 cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct
    53641 tcgatttgcc aatcgggagt cagtcaccat ccttcagtaa acggccttcc agcccgccaa
    53701 tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca ataccacccg
    53761 cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg
    53821 ctttcatcgg caatcgccag ccggtccaga cggcgttatc atccaaccgc gcttcgcaac
    53881 ctgcacccgt cagggcaaac cacccatcag tttcgaactc aactgttaac tgaccgagcg
    53941 tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca atgcgcagcg
    54001 caggcatatc cagtgcgcca cagtggctga tacccgactg gcaaaaaccg tgacgaccgc
    54061 catcctgcac agtggtgtac atgcccgcac gaataatctt cagcatattc cctccttctg
    54121 cggtacaaat cgcacgctgt ctcccggacg taataagatg ggttcgtcac gcgccggatc
    54181 aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata
    54241 aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca agcgcggttc
    54301 agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg
    54361 aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc
    54421 gctcaacccg caatacgccg cgaccaccgc caaatccggt ccgcctgcac caccgtaaac
    54481 caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct cccaccagcg
    54541 ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat
    54601 attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac gctgcgccag
    54661 tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca gcactaccgc
    54721 cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt
    54781 atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact
    54841 cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg acctgcagca taaaaatgca
    54901 atccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat
    54961 ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga
    55021 cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt cccagacgcg
    55081 cttcaatcca ggaggctagt tccattcccg gcacaggcat ggtcagctct ccccacggca
    55141 ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc tgtgcgttat
    55201 tgcccagctc aggatgtgcg tcaagcggca aatgccagcc atacaggttg atatcattcg
    55261 ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac tctcctttcc
    55321 agaagtagcc atgatgcaca atgaccgcat ctgcgcccag acgcactgcc tcatcgagca
    55381 aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg ccttccacct
    55441 gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt
    55501 gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac
    55561 atagcgttac atcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc
    55621 gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa
    55681 tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca
    55741 caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat tgtggaatta ttatgaataa
    55801 acacgcatca caaccgcgcg ctatttacta tgtcgttgcg ctgcaaatct gggaatattt
    55861 tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac taaaatacaa
    55921 cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg tcacgccaat
    55981 cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc
    56041 gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct
    56101 ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg tttaaatcta acgtaagctg
    56161 tctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt tctcgctgat
    56221 gtatgcggcg ggtaacgtgg gatctattat cgcacctatc gcctgtggtt acgcccagga
    56281 agagtacagc tgggcgatgg gctttggcct ggcggcggtt ggcatgatcg cgggtctggt
    56341 cattttctta tgtggcaatc gtcatttcac tcatacccgc ggcgttaaca aaaaagtact
    56401 gcgtgcgaca aactttctcc tgccgaactg gggatggctg ctggttctgc tggtggcaac
    56461 gcctgcactg attaccgtgc tgttctggaa agagtggtcg gtatacgcct taattgtcgc
    56521 gactattatt ggtctgggtg tactggcaaa aatttatcgc aaagcagaaa accagaaaca
    56581 gcggaaggag ctggggctga ttgtgacgct caccttcttc agtatgttgt tctgggcctt
    56641 cgcacaacag ggcggtagct cgattagcct ttatatcgac cgcttcgtta accgcgatat
    56701 gtttggttat accgttccga ccgcgatgtt ccagtcgatt aatgccttcg cagttatgct
    56761 gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg
    56821 catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac
    56881 cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg
    56941 cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt cgcaaattac
    57001 gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc tgctttctgg
    57061 cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg cttcgtttga
    57121 tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac
    57181 ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgctgaa
    57241 attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg cctgatgcga
    57301 cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat ttattgaatt tgcacaaatt
    57361 tgtaggctgg attaggcgtt cacgccgcat ccggcatctg ggctcgattg cctgacgcgt
    57421 ctgttatttc cccttccgcg ccgcctcata cgctgccaac gtttgtactc tcgcctcttt
    57481 gtgctcgact atcggttgcg gataatccag cttcacacct gctttctgcg cccacttcca
    57541 cggctcatgc accgctttcc ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa
    57601 ctcgccctca cggtcaaatt tctcgccctg agttatcgga ttgaaaatac gaaaatacgg
    57661 cgcagcatca gtacccgtag aagccgccca ctgccagcca ccgttattag ctgccaaatc
    57721 accatcaatc agctgcgaca tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc
    57781 tttcaccaga aaactggctg taatcatccg tagccggtta tgcatccagc cagtgctgtt
    57841 aagctgacgc atagcggcat caataatcgg gtatcccgtt ttgcctttct gccaggcctg
    57901 taaatgtgcg ggattgcttt gccactgcac acgatccgtc caggcaataa atggacgatg
    57961 tttacacaac gaggggtaat acgtcatcag atgacggtag aactcgcgcc agatcagctc
    58021 actaagccag acactaccgg ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg
    58081 atgcaaacac tggcgaggcg ataacccgcc cgttgccaag ctggcggaca aacggctggt
    58141 gccttccact gccggaaaat ctcgttgttg ctcatactct ccagcaccgt tctcgcaaaa
    58201 ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga
    58261 ctgacgagga taattcagcg taatggatgg cgcgggcttt atcgatccgc tactacgaac
    58321 ttttggtgca gcgacgcact ccggcatccc ttcccgcagc cgtttcagcc aggcattctt
    58381 aaaaggcgta aagactttgt acatctcgtg attgccggtc atcaccgccc ccggcggcag
    58441 gatcacgctg tcatcaaatc cttcacacac cacgttacgc agcgctcttt caacgtccac
    58501 atcccgcgct cgctcattca cctcatactg atagttataa aaaagatgag taacgctgtt
    58561 ttccgcgcac acctgtttaa ctatttcgac actggcgaca aagtcatcca cttcacggaa
    58621 caataaagga ataccttttt ccgccagcgc gatttgtagc ccattcagtt gagcattgat
    58681 aagttcagcc tgacgcggcg acatgttatg cgtctcccac tggcgtggtg tagcgatata
    58741 cagcgccagc acgcgtgcag acgaattgcg gcaggcggca gccagtgcga gattatcgtg
    58801 cagacgtaaa tcctggcgaa accaggccag atgggtagtc ataaaactcc tgaatggcgc
    58861 aagttaatca cacgcggcca aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga
    58921 taaagcgcca tatagtgttt gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg
    58981 cgataactgt cgataagcgc agccagctgc tggcgttctg tggaatcaat atgcgggcga
    59041 aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc gcgggttggc gggatgagaa
    59101 agcaaaacaa ttacgcgttg gcggtactgg ttgtaaaaat catccagatt ttgccactga
    59161 tgaatatcag ccacaaacgg gccaagtttg cggtactcgg gctgagaatg ggccagaaag
    59221 acgagtttat agcggctgtg ataatcgaga agcgccccgc gcgttaagcc atgttctttc
    59281 agtcgatgta actcatgaag ggcgaagata cggattaggg tgctgtcatc aaatcgttgt
    59341 agattcatta ctgctcctga acggtataca ggagtaagtg taggcgttgg taaatgatgt
    59401 ttgtgaagtt aatccgctag cagggcaaga ttacgaaagc gcgctccccg caagcaacta
    59461 atcaaggtag ttaatcatgg ctgaacacga tattacgcca gaagtcacca tttccaaaac
    59521 acagcgccgc tataaagtcg gctatgtcag agcccgccac caggaccgca aaaccggtat
    59581 agcccggtat tattcacaac atcccagcct gcaccttaag ggcgactggc tgaaagaggc
    59641 cggatttgat accggacggg gtgtcacggt gaagatttcg gaggggggcc tgaccatcat
    59701 cgctgacagt gacgaggtgc aggaactgcg ggatgggatg tttagtgtgc tgaacgaaag
    59761 ttaaatatta aaaaagctgg gaagatttaa aaccttccta gctttttatt ctgtcttatt
    59821 ataatggata ctcatcatct tccgatgaat ttataaattg ctccaagttt acatgaagag
    59881 tacttaacct agtttttaac tcattaagtg aagcgataat ttcatatttt ccaacataat
    59941 catatgatgg atcgtattca ttatctatgt cagaagctaa aaatgatgat attcgtagtt
    60001 taaatttagc ttcatcttta ttacattcat tggcataagg tgaatgccct atcatagcat
    60061 attcatctaa tctaaaagga taagatggtt ttaattcatc gatcaatgaa atttcaatcc
    60121 atgttctata tccaggcatt aatggactaa tttctttgag caaaaccttt ccacttaata
    60181 atgtattgag attgttcatt attatgtctc ccacgggaaa aatatatgtc taacacttcc
    60241 accatgttga tgaacttgta aatgtggtaa atcaccatca ggatttccta gtccatgacc
    60301 tgacactcta tatgaaggtt ttgttacaga aggatctttt atatcatccc aatgatatga
    60361 acctgctttt gttttatttc cagtaagata ttccattaag ttcttagtgc tggggcctga
    60421 ctcacctgtc atatttttat aattatgacc taaatatcta tccataaaca attgttcagc
    60481 gtcacttcta gacctaacag taactttggt ttcactacta tctaataaac gattcgcttt
    60541 ttggttacta acagcacatg gagttaatcc aagtggatca gcatatttta gaggattagg
    60601 tgcataactg tagagattta aaccgcctct caaccctatc gggtcagtta ccatgaaccg
    60661 cccgacatcc ggatcatagt acctgtgcag attgtagtgc agccccgttt ccctgtcgag
    60721 gtactgtccc tggtagcgca ggttctgctc caccccgccg tggtccttct cctgtatcgt
    60781 gttaccccag acctgatacc ccgtctccca cacgattttc ccgtcgctgt ccgtcatctc
    60841 ttccggcgcg ccgttcacat ccgtgtgaaa atacagtatc cggttcgggc cctctttgcc
    60901 cgccttatcc acccgcgcca gcggttcata actcccctgg tccctgtaga tgtacaggct
    60961 gcctgttccc tccggcctgc tctcctgcgc cagccgcatt ccgtcccata aaaattttgt
    61021 ccggttgtac ggcttaccgt cagggctgat gtgctgtttt tccgtccgcc tccccagtgc
    61081 atcgtacaca taccggtatg tcccccgcgc tgtcgtcact tccaccatcc ggtgttccgc
    61141 atcataacgg taactctgcg ttccgctccg gccctccttt tccaccgcac ggccaaattc
    61201 gtcataacgg tagtgcacac cacggtaaga catcagccgg ttgaacggca ccacgctgtt
    61261 ttctgccgtg gccctttccc ccgcgcgcct ccccagcagg tttcccgccg cgtcgtactg
    61321 ccagctcgcc agatacccct catcctccga acgacacgcc gtgatgcgcc ccgtggggtc
    61381 gtagaagtaa tccgtttccc cccgccggct gtgccgcgtc ttcagcagtt catcctcgcc
    61441 actgtagctg tactgccggt ccgtgaacgt ctccggtacc accccgcgca tcccgctgta
    61501 tgtctccctg cgcttcagcc gcccgccggg gtcgtaaagc tgccgcgtgc tcagtcgccc
    61561 ctggcttctg agcacttcac ggtgcaggtg gtcgcgcgtg aactcgctga tgacctcccg
    61621 gtccagattt atctgctgga gatggccgct gccgtagtac agatggcgct gcgttctgcc
    61681 gtccggaaag gtggtggcag tccggttgcc cagtttatcg tactggtggg tcaggatgcc
    61741 ctgcgccgtc tcctcgctca gcatccgccc cagtctgtcg tagctgaagc ggataacttc
    61801 cggcgcgtca gtttcgtgcc gctggcgacg gatttccagt agctggtctg ccgcatcatg
    61861 gacgtattcc gtacggccat cggctgtctc ttttgccgtg agccggcccg ccgcatcacg
    61921 cgccagccgg tggcggattt caccgccccg gtccccggtg aacaccactt ccgtgaccgc
    61981 gttcagcgcg ttgtagcggt acgactggcg gcagccgtcc gggtttatct gctccgccag
    62041 acggtccagc acatcatggc ggaaccggta gcgttctccg ttctcgttcg tgagcgtgac
    62101 cagacgcccg taagcgtcgt acccgaatgc cgtcctgcgc cccatcgcgt ccgtccggct
    62161 gcgcacccgg ccctgcccgt cccgctgcca gcgcgttatc ctccccatcg ggctggtgta
    62221 ctttaccagc ctgcccgccg cgtctgactg gtaattcacc gtgcggccgt ccgccagtat
    62281 cacctccgtc agatgaccgc tgtcgtcata cccgtagcgc gtgctgtggc tctgcgcatc
    62341 cgtcattctg ataagcctcg tgcgttcgtc gtagaaccac gccgtctgcg agccggaaca
    62401 gtccgtgtgc cgcatgagct gcccgtcttc attccactgg aggtatttat tccccccgcg
    62461 cgcatccgtt atctgcacca cctagccgtg gtggtcatac tgataccggg tgttctgctg
    62521 cagcgggtcg gtcacggcaa gaaggttgcc gcgttcatcg tacacacagc gccaggtgtt
    62581 tcccgccgcg tccacctccg tttccggctg gtgccacacc gggtgccact gcgcctgctc
    62641 cacccgcccc agcgggtcgt gcgtctccgt gatatgcccc tgcaggtcat acacatagcg
    62701 ccatttgccg ccctgagggt cggtcatccc cagcaacagt cgttcttcgt cgctccagcg
    62761 gaatgtcgtc acctgtccgg cttcgtcctg gtacgccgtc acctgattct gcgcatccca
    62821 ctggtgataa cgtgtgcgcc ccagtccgtc cgtcacgcac agcctgcgct gcgccaggtc
    62881 ccacgccagc cggtactgtt caccgctgtc cgtgtggtgc cccgtcaccc gccatgccgc
    62941 gtcatccgga ccttcatacc agccccactg gtaataatac ccttcgccgc ccggctggcg
    63001 gtgcagcgtc atgcagtggt tttcgtcgta agcaaactca cgcagcaggt gccccgtgct
    63061 gtcgtacacg ccgttcaggt ggccggcctc gtcatagctg taacgacgca gcagtaaagg
    63121 tgcagtctca gggaaatgct gataaatctg tgtcacccgc cggggatgcg ccgccagttc
    63181 gtaatacaga cggatgcacg gacgctgctg ttcgccgctg atttccacaa tgcggccccg
    63241 gtcgtcgtag tacagattca gacagttgct gttacggtcg cccagcattt tcagccgctg
    63301 gcgctgcggg tggtgcgggt cagcctcaaa cagaaaacac tgcccgtcat cgctgctgat
    63361 aagccagtgc ccctgctcac cacgccttac cgccagccct tcaccggggc tgtaaaaccc
    63421 gccccccggc agtacggctt ccatatccaa gcgtcgcccc atgcccgaca caaaggtcat
    63481 gcagttttcg tctgcgctac ccggtgtgca cacgaggctg actttataca ttacactcca
    63541 gccctgacca aacattcctt ccgtacgttc gtcacgactg ctgtacacgc gctgccagtc
    63601 cagcggaaag tggcccggca gactgaaatc gaagtcatcg tcatcggcca ggtatttggc
    63661 cccggtggcg gcattgaccg gatgcggaag ggcactggtg gccttctgcg tcagcatgct
    63721 catcccgaca ctggcagcaa aacaggcgat tttagaaagc attttgcccg ggcgaagaaa
    63781 catcagcgcc acggttgcca ccagggttat ctgcgacctg ccgcttctga tatccctgac
    63841 caccagtaac ggaccgccaa tgcgcacatc cggtgacaca aacgccccgt tccccggctc
    63901 attcaccacc ctggcctcgc aggtacagcg tgccccgctg cgtgtcgccg gctggctgtt
    63961 gatacgcaca tcccgcgcgc cttccgcgat atattccgct gcgggcggct cgtcatcgcc
    64021 tttgaacaga ttactgatgc tgccaataac cgcattcact ccctgaaagg cggtttccag
    64081 cataccgccc ggattcagca ggttaagggc cgttcccagg gggtcatcca gaaaggcggc
    64141 ttttttttgt gcgagaaact gcgtcagtga ggaaggatgt ttatggcagt cgattttatc
    64201 gtcaggggtc ggggtggatc ccggggccgc actggccaca gtgggctgcc atagttccga
    64261 cacaaaatgt gtcacgccat ccaccatttc agaacgccct tcttccgtac tgagctggtc
    64321 gtgcagagcc cgggacatca gcacaggccc tgtcaccggg ttaaccgcca gggctatggc
    64381 ccccgccatt tccagatatt ccccggccac atcccggaac gttctggcat cctcctgacg
    64441 ctgcgcttct tcctgttctg ccttcgcctg cgcttcctgc gcctctatgt cctgccgcga
    64501 tgccgcagtc gccgctgccc gcgcggcagg tttgctgttg atccatgtgt caccagagcc
    64561 ggtgaggatg taaccttcaa tctgaggcgg gaaaagcata tttgcgaggg ctgaacaggc
    64621 ttcgctgacc ttttctgtca cgccggtgta caccatcgcc acacccgcca caaaaccggt
    64681 cagcacgacg gccgtgcacc ccatcgtgat ccccactgac gtcgccagta acgaggttgt
    64741 gccgacaatg agggcatcaa taacgacatt tgcggccacc tccagcaccg cccccagcac
    64801 atctgccata aacgacgtat gcagcagggc atcaccctgt cttgccgccc agaatgcctc
    64861 ttccatgtcc cgttccctgt atgattaatt cgtcctgact tcctgccgca tcctgaagcg
    64921 cagattcttt ttaatttcag cccagcgcgt catatctgcc ggggtcagcg gtgtggttct
    64981 ggcgtaggtg aaggccatca tgacccgctc acccggcaat acaaatgcca cctgacactg
    65041 gtacacctgc tcgttacccc ggagaaactg gctctgtatt tccaaaccgc ctcgttgaga
    65101 taatattccg tcataatgta aatagtccgt tttaataaag gaattattta ttgacaggtt
    65161 ctgttttttt aatcatgaat gtaataccca tcaccgcttt acagcacact ttatctttcc
    65221 agccataatc actaataata ttaagcaatt agcaatattt acaacgacca gagcggatac
    65281 atagttatgt atttaacaat cccaatatat tcactatgca aataaaacca ctagtaacag
    65341 acacgctacc gcctccatat aaaaatggac accttataaa attgtatctt gcatagaaaa
    65401 agctgaaata tcggatgcgt tttaattatc aactataaat ctataagctt tttattaaca
    65461 a
//
LOCUS       DAJTNV010000010        60258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNV010000010 DAJTNV010000000
VERSION     DAJTNV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..322
                     /locus_tag="OO199_000624"
     CDS             <1..322
                     /locus_tag="OO199_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5534787.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO199_000625"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO199_000625"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5534788.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO199_000626"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO199_000626"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5534789.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO199_000627"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO199_000627"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5534790.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO199_000628"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO199_000628"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5534791.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO199_000629"
     CDS             complement(3726..4955)
                     /locus_tag="OO199_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5534792.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO199_000630"
     CDS             complement(5019..5459)
                     /locus_tag="OO199_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5534793.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO199_000631"
     CDS             complement(5564..6334)
                     /locus_tag="OO199_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5534794.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO199_000632"
     CDS             complement(6331..7257)
                     /locus_tag="OO199_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5534795.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO199_000633"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO199_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5534796.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYSIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO199_000634"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO199_000634"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5534797.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO199_000635"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO199_000635"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5534798.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO199_000636"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO199_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5534799.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO199_000637"
     CDS             12964..13311
                     /locus_tag="OO199_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5534800.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO199_000638"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO199_000638"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5534801.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO199_000639"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO199_000639"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5534802.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO199_000640"
     CDS             complement(15131..15493)
                     /locus_tag="OO199_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5534803.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO199_000641"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO199_000641"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5534804.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO199_000642"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO199_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO199_000643"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO199_000643"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5534805.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO199_000644"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO199_000644"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5534806.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO199_000645"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO199_000645"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5534807.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO199_000646"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO199_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5534808.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHSESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO199_000647"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO199_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5534809.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO199_000648"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO199_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5534810.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO199_000649"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO199_000649"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5534811.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO199_000650"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO199_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5534812.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO199_000651"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO199_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5534813.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO199_000652"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO199_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO199_000653"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO199_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5534814.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO199_000654"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO199_000654"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5534815.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO199_000655"
     CDS             complement(36348..36386)
                     /locus_tag="OO199_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534816.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO199_000656"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO199_000656"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5534817.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO199_000657"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO199_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5534818.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO199_000658"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO199_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5534819.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO199_000659"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO199_000659"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5534820.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO199_000660"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO199_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5534821.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO199_000661"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO199_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5534822.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO199_000662"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO199_000662"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5534823.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO199_000663"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO199_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5534824.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO199_000664"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO199_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5534825.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO199_000665"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO199_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5534826.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO199_000666"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO199_000666"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5534827.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO199_000667"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO199_000667"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5534828.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO199_000668"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO199_000668"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5534829.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO199_000669"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO199_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5534830.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO199_000670"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO199_000670"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5534831.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO199_000671"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO199_000671"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5534832.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO199_000672"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO199_000672"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5534833.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDLADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO199_000673"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO199_000673"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5534834.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO199_000674"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO199_000674"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5534835.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO199_000675"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO199_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5534836.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO199_000676"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO199_000676"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5534837.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO199_000677"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO199_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5534838.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14422 a        16708 c        14817 g        14311 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacag cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcagaatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactggac gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaagat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac agctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgactctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaa
//
LOCUS       DAJTNV010000011        59728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNV010000011 DAJTNV010000000
VERSION     DAJTNV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            530..1819
                     /gene="sdaC"
                     /locus_tag="OO199_000678"
     CDS             530..1819
                     /gene="sdaC"
                     /locus_tag="OO199_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5534839.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1877..3244
                     /gene="sdaB"
                     /locus_tag="OO199_000679"
     CDS             1877..3244
                     /gene="sdaB"
                     /locus_tag="OO199_000679"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5534840.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3355..4110
                     /gene="xni"
                     /locus_tag="OO199_000680"
     CDS             3355..4110
                     /gene="xni"
                     /locus_tag="OO199_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5534841.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4163..5311)
                     /gene="fucO"
                     /locus_tag="OO199_000681"
     CDS             complement(4163..5311)
                     /gene="fucO"
                     /locus_tag="OO199_000681"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5534842.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5339..5986)
                     /gene="fucA"
                     /locus_tag="OO199_000682"
     CDS             complement(5339..5986)
                     /gene="fucA"
                     /locus_tag="OO199_000682"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5534843.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6533..7849
                     /gene="fucP"
                     /locus_tag="OO199_000683"
     CDS             6533..7849
                     /gene="fucP"
                     /locus_tag="OO199_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5534844.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7882..9657
                     /gene="fucI"
                     /locus_tag="OO199_000684"
     CDS             7882..9657
                     /gene="fucI"
                     /locus_tag="OO199_000684"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5534845.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9766..11185
                     /gene="fucK"
                     /locus_tag="OO199_000685"
                     /pseudo
     CDS             9766..11185
                     /gene="fucK"
                     /locus_tag="OO199_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11187..11609
                     /gene="fucU"
                     /locus_tag="OO199_000686"
     CDS             11187..11609
                     /gene="fucU"
                     /locus_tag="OO199_000686"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5534846.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11667..12398
                     /gene="fucR"
                     /locus_tag="OO199_000687"
     CDS             11667..12398
                     /gene="fucR"
                     /locus_tag="OO199_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5534847.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12442..13542)
                     /gene="rlmM"
                     /locus_tag="OO199_000688"
     CDS             complement(12442..13542)
                     /gene="rlmM"
                     /locus_tag="OO199_000688"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5534848.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13535..13930)
                     /locus_tag="OO199_000689"
     CDS             complement(13535..13930)
                     /locus_tag="OO199_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5534849.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13949..14866)
                     /gene="gcvA"
                     /locus_tag="OO199_000690"
     CDS             complement(13949..14866)
                     /gene="gcvA"
                     /locus_tag="OO199_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5534850.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15217..15444)
                     /locus_tag="OO199_000691"
     CDS             complement(15217..15444)
                     /locus_tag="OO199_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5534851.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15636..16841
                     /gene="csdA"
                     /locus_tag="OO199_000692"
     CDS             15636..16841
                     /gene="csdA"
                     /locus_tag="OO199_000692"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5534852.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16841..17284
                     /gene="csdE"
                     /locus_tag="OO199_000693"
     CDS             16841..17284
                     /gene="csdE"
                     /locus_tag="OO199_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5534853.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17335..18141)
                     /gene="tcdA"
                     /locus_tag="OO199_000694"
     CDS             complement(17335..18141)
                     /gene="tcdA"
                     /locus_tag="OO199_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5534854.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18380..19477)
                     /gene="mltA"
                     /locus_tag="OO199_000695"
     CDS             complement(18380..19477)
                     /gene="mltA"
                     /locus_tag="OO199_000695"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5534855.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19686..19762
                     /locus_tag="OO199_000696"
     tRNA            19686..19762
                     /locus_tag="OO199_000696"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19720..19722,aa:Met,seq:cat)
     gene            complement(19837..21090)
                     /gene="amiC"
                     /locus_tag="OO199_000697"
     CDS             complement(19837..21090)
                     /gene="amiC"
                     /locus_tag="OO199_000697"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5534856.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21322..22653
                     /gene="argA"
                     /locus_tag="OO199_000698"
     CDS             21322..22653
                     /gene="argA"
                     /locus_tag="OO199_000698"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5534857.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22715..24541)
                     /gene="recD"
                     /locus_tag="OO199_000699"
     CDS             complement(22715..24541)
                     /gene="recD"
                     /locus_tag="OO199_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5534858.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24541..28083)
                     /gene="recB"
                     /locus_tag="OO199_000700"
     CDS             complement(24541..28083)
                     /gene="recB"
                     /locus_tag="OO199_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5534859.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28076..30964)
                     /gene="ptrA"
                     /locus_tag="OO199_000701"
     CDS             complement(28076..30964)
                     /gene="ptrA"
                     /locus_tag="OO199_000701"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5534860.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31140..34508)
                     /gene="recC"
                     /locus_tag="OO199_000702"
     CDS             complement(31140..34508)
                     /gene="recC"
                     /locus_tag="OO199_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5534861.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34521..34853)
                     /locus_tag="OO199_000703"
     CDS             complement(34521..34853)
                     /locus_tag="OO199_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5534862.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34838..35245)
                     /locus_tag="OO199_000704"
     CDS             complement(34838..35245)
                     /locus_tag="OO199_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5534863.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35242..35805)
                     /locus_tag="OO199_000705"
     CDS             complement(35242..35805)
                     /locus_tag="OO199_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5534864.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35796..36266)
                     /locus_tag="OO199_000706"
                     /pseudo
     CDS             complement(35796..36266)
                     /locus_tag="OO199_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36450..37244)
                     /gene="thyA"
                     /locus_tag="OO199_000707"
     CDS             complement(36450..37244)
                     /gene="thyA"
                     /locus_tag="OO199_000707"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5534865.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37251..38126)
                     /gene="lgt"
                     /locus_tag="OO199_000708"
     CDS             complement(37251..38126)
                     /gene="lgt"
                     /locus_tag="OO199_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5534866.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38277..40523)
                     /gene="ptsP"
                     /locus_tag="OO199_000709"
     CDS             complement(38277..40523)
                     /gene="ptsP"
                     /locus_tag="OO199_000709"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5534867.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40536..41066)
                     /gene="rppH"
                     /locus_tag="OO199_000710"
     CDS             complement(40536..41066)
                     /gene="rppH"
                     /locus_tag="OO199_000710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5534868.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41751..42440
                     /gene="mutH"
                     /locus_tag="OO199_000711"
     CDS             41751..42440
                     /gene="mutH"
                     /locus_tag="OO199_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5534869.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42509..43222
                     /locus_tag="OO199_000712"
     CDS             42509..43222
                     /locus_tag="OO199_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5534870.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43360..43578
                     /gene="ygdR"
                     /locus_tag="OO199_000713"
     CDS             43360..43578
                     /gene="ygdR"
                     /locus_tag="OO199_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5534871.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43686..44726
                     /locus_tag="OO199_000714"
     CDS             43686..44726
                     /locus_tag="OO199_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5534872.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44759..45949)
                     /gene="lplT"
                     /locus_tag="OO199_000715"
     CDS             complement(44759..45949)
                     /gene="lplT"
                     /locus_tag="OO199_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5534873.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45942..48101)
                     /gene="aas"
                     /locus_tag="OO199_000716"
     CDS             complement(45942..48101)
                     /gene="aas"
                     /locus_tag="OO199_000716"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5534874.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48686..49717
                     /gene="galR"
                     /locus_tag="OO199_000717"
     CDS             48686..49717
                     /gene="galR"
                     /locus_tag="OO199_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5534875.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49724..50986)
                     /gene="lysA"
                     /locus_tag="OO199_000718"
     CDS             complement(49724..50986)
                     /gene="lysA"
                     /locus_tag="OO199_000718"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5534876.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51108..52043
                     /gene="lysR"
                     /locus_tag="OO199_000719"
     CDS             51108..52043
                     /gene="lysR"
                     /locus_tag="OO199_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5534877.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52030..52722)
                     /gene="ygeA"
                     /locus_tag="OO199_000720"
     CDS             complement(52030..52722)
                     /gene="ygeA"
                     /locus_tag="OO199_000720"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5534878.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52851..54269)
                     /gene="araE"
                     /locus_tag="OO199_000721"
     CDS             complement(52851..54269)
                     /gene="araE"
                     /locus_tag="OO199_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5534879.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54584..55345)
                     /gene="kduD"
                     /locus_tag="OO199_000722"
     CDS             complement(54584..55345)
                     /gene="kduD"
                     /locus_tag="OO199_000722"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5534880.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55375..56211)
                     /gene="kduI"
                     /locus_tag="OO199_000723"
     CDS             complement(55375..56211)
                     /gene="kduI"
                     /locus_tag="OO199_000723"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5534881.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56498..57679)
                     /locus_tag="OO199_000724"
     CDS             complement(56498..57679)
                     /locus_tag="OO199_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5534882.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57934..59163
                     /locus_tag="OO199_000725"
                     /pseudo
     CDS             57934..59163
                     /locus_tag="OO199_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14746 a        16025 c        14540 g        14417 t
ORIGIN      
        1 ccgggttttc tcgtttttgg tcacttactc atcaactcat ttcatttgtt atatgaatgt
       61 ttcttaccac cctcacgcga caaatatcat cacagttaat atgtcataca atttatgttg
      121 caacacaaac gtttccctat tttcataaac cgtattttat cgctatgaaa aagaaattat
      181 cgccatgatt aactaaaagt attgattttt tcagttcaac ctacatatat tgcgcgcccc
      241 ggaagaagtc agatgtcgtt taatgggcaa atattgccct taaattctct tttacttttg
      301 atttacagag taaagcgttg ggataatcta tcttccaagc agattattgt atttgggatc
      361 aagatcactg atagatacat aacttgtgtg tatctttccg ccctcaaatt attacggcgg
      421 taaatgatta agccatcgcc gatagacaga tttcattttt acggtcaggc accttccctg
      481 gctgaactgg ctaaaagctg aattatttgc attcctccag gagaaataga tggaaacgac
      541 tcaaaccagc acgattgcgt cgaaagactc tcgtagtgcc tggcgcaaga cagacaccat
      601 gtggatgctg ggcctttacg gcacggcaat cggcgcgggc gtgctgttcc tgccaatcaa
      661 cgccggtgtt ggcggtatga tcccgctgat catcatggct atccttgcgt tcccgatgac
      721 gttttttgct caccgcggcc tgactcgctt cgtactgtct ggtaaaaacc cgggcgaaga
      781 catcaccgag gttgtagaag aacactttgg tattggcgca ggtaaactga ttaccctgct
      841 ctacttcttc gctatctacc cgatcctgct ggtttatagc gtggcaatca ccaataccgt
      901 tgaaagcttc atgtctcacc agctgggtat gacgccaccg ccgcgtgcga ttctgtcgct
      961 gatcctgatc gtgggtatga tgaccatcgt tcgctttggt gagcagatga tcgttaaagc
     1021 gatgagtatt ctggtattcc cgtttgttgg cgtactgatg ctgctggctc tgtacctgat
     1081 cccgcagtgg aacggcgcag cactggaaac gctgtctctg gacactgcat ctgcaaccgg
     1141 aaacggtctg tggatgaccc tgtggctggc aattccggta atggtgttct cgttcaacca
     1201 ctctccgatc atctcttctt tcgccgttgc gaagcgtgaa gagtacggcg atatggcaga
     1261 acagaaatgc tcgaagatcc tggcattcgc acacatcatg atggtgctga ccgtaatgtt
     1321 cttcgtcttc agctgcgtac tgagcctgac tccggcagac ctggctgcgg ctaaagagca
     1381 gaacatctcg attctgtctt acctggctaa ccactttaac gcaccggtta tcgcgtggat
     1441 ggctccgatt atcgcgatta tcgctatcac caaatccttc ctcggccact acctgggcgc
     1501 acgtgaaggc ttcaacggta tggtgattaa atctctgcgt ggtaaaggta agtctatcga
     1561 aatcaacaag ctgaaccgta tcactgcgct gttcatgctg gtaacgacct ggattgttgc
     1621 caccctgaac ccgagcatcc tgggtatgat tgaaaccctg ggcggcccaa tcatcgcgat
     1681 gatcctgttc ctgatgccga tgtacgcaat tcagaaagta ccggcaatgc gtaagtacag
     1741 cggtcacatc agcaacgtat tcgttgtcgt gatgggtctg attgcaatct ccgcaatctt
     1801 ctactctctg ttcagctaag tcctttcgcg ccgctttcgg gcggcgcttc ctccgtttta
     1861 acgcgatgta tttcctatga ttagcgtatt cgatattttc aaaatcggca ttggcccttc
     1921 cagttctcat accgttggac caatgaaagc gggtaaacaa tttaccgacg atctgattgc
     1981 ccgtaacctg cttaaagacg tgacccgcgt ggtggttgac gtgtacggct cgctctctct
     2041 gactggtaaa ggccaccaca cagatatcgc cattattatg ggcctggcgg gtaacctgcc
     2101 ggataccgtg gatatcgatt ccatccccgg ttttattcag gatgtgaata ctcatggtcg
     2161 cctgatgctg gcaaacggtc agcatgaagt ggagttcccg gttgatcagt gcatgaactt
     2221 ccatgcagat aacctttctc tgcatgaaaa cggtatgcgc attaccgcgc tggcgggcga
     2281 taaagtcgtt tacagccaga cttactactc tattggcggt ggctttatcg ttgatgaaga
     2341 gcattttggc cagcaggata gcgcaccggt tgaagttcct tatccgtaca gttcagcagc
     2401 cgatctgcaa aaacattgtc aggaaaccgg gctgtcactc tctggcctga tgatgaaaaa
     2461 cgagctggcg ctgcacagta aagaagagct ggaacagcac ctggcgaacg tctgggaagt
     2521 catgcgtggc ggtattgagc gcggtatttc caccgaaggc gtgttgcctg gcaaactgcg
     2581 cgttccacgc cgtgctgcgg cactacgccg gatgctggtc agcctggata aaaccaccac
     2641 tgacccgatg gcggttgttg actggatcaa catgtttgca ctggcagtga acgaagagaa
     2701 cgctgctggc ggtcgcgtgg tgactgcgcc gactaacggt gcgtgcggga ttatcccggc
     2761 tgtgctggcg tactacgata agtttatccg cgaagtgaac gctaactcac tggctcgtta
     2821 cctgctggta gccagcgcca ttggttctct ttataagatg aacgcgtcga tttctggtgc
     2881 cgaagtgggt tgccagggtg aagttggcgt ggcgtgctct atggcggcag ctggtctggc
     2941 agaactgtta ggcgcaagcc cggcgcaggt ttgcatcgca gcggaaatcg cgatggagca
     3001 caacctcggt ctgacttgtg acccggtcgc ggggcaggtt caggtaccgt gcattgagcg
     3061 taacgccatt gcagcagtga aagcggtgaa cgccgcgcgt atggcactgc gccgtaccag
     3121 cgaaccgcgc gtctgcctcg ataaagttat cgaaaccatg tacgaaacag gtaaagacat
     3181 gaacgccaag taccgcgaaa cctctcgcgg cggcctggcg atgaagatcg ttgcctgcga
     3241 ttaatctctc ccagaggcct cgttttgcga agcctcttcc tgttttctca accagccata
     3301 gcctgttccg gcatcgaatg ttacccttat cgcctgatct ttaagggggt tatcgtggct
     3361 gttcatttgc ttattgtcga tgcactgaat cttatccgtc gcattcatgc cgttcagggt
     3421 tcgccctgtg tcgaaacctg ccagcatgcg ctcgatcagc tcattatgca cagccagcca
     3481 acccacgcgg tcgccgtttt tgatgatgaa aaccgcagta gcggctggcg tcatcagcgt
     3541 ttaccagatt acaaagcgga tcgaccgcca atgccggaag agttgcacga cgagatgcct
     3601 gcattacgcg ccgcctttga gcaacgcggc gtcccgtgct ggtcagccag cggcaacgaa
     3661 gccgatgact tagccgccac actggcggtc aaagtgacac aggccgggca tcaggcaacg
     3721 attgtttcga cagataaagg ctactgtcag ttactttcac cgacattacg tattcgtgat
     3781 tacttccaga aacgttggct ggatgcgcca tttatcgata aagaatttgg cgttcaaccg
     3841 cagcagttgc ccgattactg gggacttgcg gggatcagca gttcaaaggt accgggtgtt
     3901 gcgggaatcg gaccaaaaag cgccacgcag ctgctggtcg agtttcagag tctggaaggg
     3961 atatatgaga atctggatgc ggttgccgaa aagtggcgca aaaaattaga aacccataaa
     4021 gagatggcgt ttctgtgccg cgatattgcc cgcttacaaa ccgatttgca tatcgacggc
     4081 aatttacagc aattgcggtt ggtacggtaa cggcgagccg gatacgccgc aaatcgtatc
     4141 cggcattatc acatcagcgc atttaccagg cggtatggta aagctctaca atatcctcaa
     4201 gcgttgcttc acgcgggttg ccaccggtac aaacatcatc cagtgccgcc tgagccagtg
     4261 ccgaaatgtc ttccttgcgt acaccaacat cacgcaaatg tggcggaata ccgacatcac
     4321 ggttgagggc aaacaccgct tcaacagcgg cattacgcgc cccttccagg ctcatacctt
     4381 ccactttcac gcccataacg cgcgcgatat cgcggtactt ctcaccggta aagtcagcgt
     4441 tatagcgcat gacatgcggt aacaggatgg cgttcgcaac accgtgtgga gtgttataaa
     4501 acgcgcccag tggatgcgcc ataccatgca ccaaccctaa cccaacattc gagaagccca
     4561 tacccgcaac atactgcccg agcgccattt cttctccggc atccttatca ccagcaaccg
     4621 atcctcgcag cgccccagca atgatttcaa tcgctttaat gtgcagtgca tcggttagtg
     4681 cccacgcgcc acgggtaata tacccctcaa tagcatgagt gagcgcatca acacccgtcg
     4741 cggctttcag cgccggaggc ataccatcca tcatgtcagc atcaataaac gccacctgcg
     4801 ggatatcatg cggatcaacg caaacaaact tgcgccgttt ttcttcgtca gtgatcacgt
     4861 agttaatggt cacttctgcc gcagtacctg ctgtggtggg gattgccaga atcggtacac
     4921 tgggtttatt ggtcggggaa agcccttcca ggctacgcac atcggcaaac tccgggttgt
     4981 tgctgataat gccaattgct ttacaagtat cctgtggaga accaccacca atagcgatca
     5041 ggtaatccgc gccgctattc tggaatacac cgagcccttc tttgacgaca gtaattgttg
     5101 ggttgggcac tacgccgtcg taaatcgccc atgccagccc tgcagcatcc atcttatcgg
     5161 tcactttcgc caccacgccg cattgcacca gcgttttatc ggtgacgatc agcgccttct
     5221 gataaccacg gcgtttcacc tcatcggtta aagccccaac agcaccccga ccaaaccatg
     5281 ccgtttcgtt cagaatcatt ctgttagcca tcatccttct ccttgttgct ttacgaaatt
     5341 actcttcaat ccgtaaccca taggttttga atttctccag cactacggca atctcttcat
     5401 cgctcagcac tggcaccggg tccgtaatcg ccagggtcgt caggtaaagt tgcgccagca
     5461 cttcaacttc atgcgccagc cataacgctt tttccagatt cacctcacaa gcgataagcc
     5521 catgatgttg taacaaagtt gccttacgat ttttgagagc cagcgcaaca tgttcagaaa
     5581 gttcgcgtgt tccaaaggtc gcataaggcg cgcaaggaat agaattaccg ccagccgccg
     5641 caatcatgta gtgaatagcg gggatcgatc ggttaagaat ggaaactgcc gtgcaatgaa
     5701 cggcatgatt gtgaacaacc gcgttggcat ccggtctgct ttgataggct gccatatgga
     5761 aacgccattc gcttgagggg agctttcctt cctcatgttt accgttgcca tcaataaaga
     5821 caatatgcga ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc agcatcccat
     5881 cctgataacg tacactgacg ttccccgctg tcccctggtt cagtcccagg cgggtcattt
     5941 ccaggcaagt gtcaataatc tgacgagcaa gtttatttcg ttccattagc tacctctctc
     6001 tgattcaaaa cagggcaata atgttgttcc tttcacacta ttgaattagc cgtttaatta
     6061 cccaccatct tcttcctgat taacaagaaa gaaattcaca agcttatatt ttgtgaccta
     6121 gctcaactaa tcacagtaaa taactgcaag ttctcttttt ataaccccgt taaaaatgac
     6181 cgctcttaaa aatatttatc aaaacggtca tttttccatt tcccctcgcc agaaatgccc
     6241 gttttcggca caaacaatta atacggtcat ctggtttgtg ttttttatga tttattttct
     6301 gaaacgggca tgaaatttcg attattaaag tgatggtagt cacataaagt caccttccag
     6361 ctaataagtg tgaccgccgt catattacag agcgtttttt atttgaaaat gaatccatga
     6421 gttcatttca gacaggcaaa tattcaccga tatgaagccc gaactcgctg gttttgcact
     6481 tttgaaaaca taaccgatta cgtgcttaag cttctgaacc taagaggatg ctatgggaaa
     6541 cacatcaata caaacgcaga gttaccgtgc ggtagataaa gatgcagggc aaagcagaag
     6601 ttacattatt ccattcgcgc tgctgtgctc actgtttttt ctttgggcgg tagccaataa
     6661 ccttaacgac attttattac ctcaattcca gcaggctttt acgctgacaa atttccaggc
     6721 tggcctgatc caatcggcct tttactttgg ttatttcatt atcccaatcc ctgctgggat
     6781 attgatgaaa aaactcagtt ataaagcagg gattattacc ggattatttt tatatgcctt
     6841 gggcgctgca ttattctggc ccgctgcaga aataatgaac tacaccttat ttttagttgg
     6901 cctatttatt attgcagccg gattaggttg tctggaaact gccgcaaacc cttttgttac
     6961 ggtattaggg ccggaaagca gtagtcactt ccgcttaaat cttgcgcaaa catttaactc
     7021 gtttggcgca attatcgcgg ttgtctttgg gcaaagtctt attttgtcta acgtgccaca
     7081 tcaatcgcaa gacgttctcg ataaaatgtc tccagagcaa ttgagtgcgt ataaacacag
     7141 cctggtatta tcggtacaga caccttatat gatcatcgtg gctatcgtgt tactggtcgc
     7201 cctgctgatc atgctgacga aattcccggc attgcagagt gataatcaca gtgacgccaa
     7261 acaaggatcg ttctccgcat cgctttctcg tctggcgcgt attcgtcact ggcgctgggc
     7321 ggtattagcg caattctgct atgtcggcgc acaaacggcc tgctggagct atttgattcg
     7381 ctacgctgta gaagaaattc caggtatgac tgcaggcttt gccgctaact atttaaccgg
     7441 aaccatggtg tgcttcttta ttggtcgttt caccggtacc tggctcatca gtcgcttcgc
     7501 accacacaaa gtcctggccg cctacgcatt aatcgctatg gcactgtgcc tgatctcagc
     7561 cttcgctggc ggtcatgtgg gcttaatagc cctgacttta tgcagcgcct ttatgtcgat
     7621 tcagtaccca acaatcttct cgctgggcat taagaatctc ggccaggaca ccaaatacgg
     7681 ttcgtccttc atcgttatga ccattattgg cggcggtatt gtcactccgg tcatgggttt
     7741 tgtcagtgac gcggcgggca acatccccac tgctgaactg atccccgcac tctgcttcgc
     7801 ggtcatcttt atctttgccc gtttccgttc tcaaacggca actaactgaa catattttcc
     7861 gaataaagtg aggaatctgt aatgaaaaaa atcagcttac cgaaaattgg tatccgcccg
     7921 gttattgacg gtcgtcgcat gggtgttcgt gagtcgcttg aagaacaaac aatgaatatg
     7981 gcgaaagcta cggccgcact gctgaccgag aaactgcgcc atgcctgcgg agctgccgtc
     8041 gagtgtgtca tttccgatac ctgtatcgcg ggtatggctg aagccgctgc ttgcgaagaa
     8101 aaattcagca gccagaatgt aggcctcacc attacggtaa cgccttgctg gtgctatggc
     8161 agtgaaacca tcgacatgga tccaacccgc ccgaaagcca tttggggctt taacggcact
     8221 gaacgccccg gcgctgttta cctggcagcg gctctggcag ctcacagcca gaaaggcatc
     8281 ccggcattct ccatttacgg tcatgacgtt caggatgccg atgacacatc gattcctgcc
     8341 gatgttgaag aaaaactgct gcgctttgcc cgcgccggtt tggccgtcgc cagcatgaaa
     8401 ggtaaaagct atctgtcgct gggcggcgtt tcgatgggta tcgccggttc tattgttgat
     8461 cacaacttct ttgaatcctg gctgggaatg aaagtccagg cggtggatat gaccgaactg
     8521 cgtcgccgta tcgatcagaa gatttacgac gaagccgaac tggaaatggc actggcctgg
     8581 gctgataaaa acttccgcta tgacgaagat gaaaataaca aacagtatca acgcaatgcc
     8641 gagcaaagcc gcgcagttct gcgcgaaagt ttactgatgg cgatgtgtat ccgcgacatg
     8701 atgcaaggca acagcaaact ggccgatatt ggtcgcgtgg aagaatcact tggctacaac
     8761 gccatcgctg cgggcttcca ggggcaacgt cactggaccg atcaatatcc caatggtgac
     8821 accgccgaag cgatcctcaa cagttcattt gactggaatg gcgtgcgcga accctttgtc
     8881 gtggcgaccg aaaacgacag tcttaacggc gtggcaatgc taatgggtca ccagctcacc
     8941 ggcaccgctc aggtatttgc cgatgtgcgt acctactggt caccagaagc aattgagcgt
     9001 gtaacggggc ataaactgga cggactggca gaacacggca tcatccattt gatcaactca
     9061 ggttctgctg cgctggacgg ttcctgtaaa caacgcgaca gcgaaggtaa cccgacgatg
     9121 aagccgcact gggaaatctc tcagcaagag gctgacgctt gcctcgccgc taccaaatgg
     9181 tgcccggcga tccacgaata cttccgtggc ggcggttact cttcccgctt ccttaccgaa
     9241 ggcggcgtcc cgttcaccat gactcgtgtc aacatcatca aaggcctggg accggtactg
     9301 caaatcgcgg aaggctggag cgtggaattg ccgaaggatg tgcatgacat cctcaacaaa
     9361 cgcaccaact caacctggcc aaccacctgg tttgcaccgc gcctcactgg taaagggccg
     9421 tttacggatg tgtattcggt aatggcgaac tggggtgcca accacggagt tctgaccatc
     9481 ggccacgttg gcgcagactt tatcaccctt gcctccatgc tgcgtatccc ggtatgtatg
     9541 cacaacgttg aagagaccaa agtgtatcgt ccttctgcct gggctgcgca cggcatggat
     9601 attgaaggcc aggattaccg cgcttgccag aactacggtc cgttgtacaa gcgttaatac
     9661 attacaccct gatgtgatgc ccggtcgctc cggctaccgg gcctgaacaa gcaagagtgg
     9721 ttagccggat aaacaatgtt atccggctat attgcaggag cgattatgaa acaagaagtt
     9781 atcctggtac tcgactgtgg cgcgaccaat gtcagggcca tcgcggttaa tcggcaggga
     9841 aaaattgttg cccgcgcctc aacgcctaat gccagcgata tcgcgatgga aaacaacacc
     9901 tggcaccagt ggtctttaga ctgccatttt gcaacgcttt gctgattgct gtcggcaaat
     9961 caatagtgaa ctgactgaat gccacatccg cggtatcgcc gtcaccacct ttagtgtgga
    10021 tggcgctctg gtagataagc aaggcaatct gctctatccg atcattagct ggaaatgtcc
    10081 gcgaacagca gcggttatgg acaatattga acggttaatc tccgcacagc ggttgcaggc
    10141 tatttctggc gtcggagcct ttagtttcaa tacgttatat aagttggtgt ggttgaaaga
    10201 aaatcatcca caactgctgg aacgcgcgca cgcctggctc ttcatttcgt cgctgattaa
    10261 ccaccgttta accagcgaat tcactactga tatcacgatg gccggaacca gccagatgct
    10321 ggatatccag caacgcgatt tcagtccgca aattttacaa gccaccggta ttccacgccg
    10381 actcttccct cgtctggtgg aagcgggtga acagattggt acgctacaga acaacgccgc
    10441 agcaatgctc ggcttacccg tcggcatacc ggtgatttcc gcaggtcacg atacccagtt
    10501 cgcccttttt ggcgctggtg ccgaacaaaa tgaacccgtg ctctcctccg gtacatggga
    10561 aattttaatg gttcgcagcg cctaggttga tacttcgctg ttaagtcagt acgccggttc
    10621 cacctgcgaa ctagatagcc aggcatggtt gtataaccca ggtatgcaat ggctggcatc
    10681 cggcgtgctg gaatgggtga gaaaactgtt ctggacggct gaaacaccct ggcaaatgtt
    10741 gattgaagaa gctcgtctga tcgcacctgg cgcggatggt gtgaaaatgc agtgtgattt
    10801 attgtcgtgt cagaacgctg gctggcaagg agtgacgctt aataccacgc gggggcattt
    10861 ctatcgcgcg gcgctggaag ggttaactgc gcaattacag cgcaatctac agatgctgga
    10921 aaaaatcggg cactttaaag cctatgaatt attgttagtc ggtggaggaa gtcgcaacac
    10981 attgtggaat cagattaaag ccaatatgct tgatattccg gtaaaagttc tcgacgatgc
    11041 cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat ggcgtagggg aatttaacag
    11101 cccggaagaa gcccgtgcac agattcatta tcagtaccgt tatttctacc cgcaaactga
    11161 acctgaattt atagaggaag tgtgaaatgc tgaaaacaat ttcgccgtta atttctcccg
    11221 aactattgaa agtgctggca gagatgggac atggagatga aattattttt tccgatgctc
    11281 actttcccgc ccattcgatg gaaccgcagg tgatccgcgc tgatggcctg ttggtgagcg
    11341 acttgctcca ggcgattatc ccgttatttg agctggacag ttatgccccg ctgctggtga
    11401 tgatggcggc ggtagagggt gacactctcg atcctgaagt agaacgacgt taccgtaatg
    11461 cgctttcact acaagccccg tgtcctgaca tcatccgcat caatcgtttt gcgttttatg
    11521 aacgggcgca aaaagccttt gcgatcgtta tcacaggcga acgagcgaag tacgggaata
    11581 ttcttttaaa aaaaggggta acaccgtaat ctcataccgg tacgcccgca tgacgcgggc
    11641 ggttatcgaa tgatggggtg aaaaatatga aagcggcacg ccagcaagcg atagtcgacc
    11701 tgctgctgaa ccataccagc ctgaccacgg aagctctctc tgaacagcta aaggtcagta
    11761 aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca gggtaaaatt ctgcgcaatc
    11821 atggacgcgc taaatatatc caccgtcaaa atcaagacag tggcgatccc tttcacatca
    11881 ggctgaaaag ccattatgcg cataaagcag atatcgcgcg cgaggcgctc gcgtggattg
    11941 aagaagggat ggtgatagcc ttagacgcca gttcaacttg ctggtatctg gcacgccagt
    12001 tgcctgacat caacattcag gtcttcacca atagccatcc gatttgccat gaactcggta
    12061 aacgcgaacg cattcaactg atcagttccg gcggcacact tgagcgcaaa tatggctgtt
    12121 acgtcaatcc ctcgctgatt tcccaactta aatcgctgga aatcgatctg tttatttttt
    12181 cttgtgaagg gatcgatagc agcggcgcac tgtgggactc caatgcgatc aacgctgatt
    12241 acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt attgattgat aaaagtaaat
    12301 ttaatcgttc aggggaagcc cgcatcgggc atctggatga ggtaacgcac attatttctg
    12361 atgagcgcca ggttgcaact tctttggtaa cagcctgacg gaaagggtag caggccggag
    12421 acgacgcccc ggccttgcct gttatcgctc gtcgcgacga ccacccaccg cagcccagat
    12481 gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac aactgccgcg cctgagtctg
    12541 agcatttatg ccatgttcat caagctgtgc ctgaatatac gccagattgt gtgatacttc
    12601 ttcgtagcgt tttttcatcg gcagtttgag attgaaaatg gtttcacggc accagccatt
    12661 aaccagccac tgcgccatca atgccgcaac tttagccggt ttttcaacca tatcgcatac
    12721 catccaggag atattgctgc gtgtcggacg gaatttgaaa ccgtcttccc gcagccacgt
    12781 cacctgcccg gtatccatca gactttgcgc catcgggccg ttgtcgacgg aataaaccca
    12841 catgttgcgc ttcaccagtt ggtaggtcca gccgccaggg caagcgccta aatccaccgc
    12901 ccacatcccg ttcgccaggc gttcatccca ctcatccgca ggaataaaca catgaaatgc
    12961 ctcttccagt ttgagcgtgg aacgactcgg cgcctctgcc ggaaatttca ggcgcggaat
    13021 gcccatatag aacggcgaat tattgttgct gtatgagtaa ccggtatagc agcatcctgg
    13081 tgcaataaag aatacatgca caaccggacg cttcggcgtt tcatagttcg ccagcacccc
    13141 agcatcgcgc aaggcagcgc gtagcggaac agtgaactta cggcagaatt tcagcaactc
    13201 tttgctttcg ttggtatcgg caacttcaac acgcagttca ccgcccttct ctactacacc
    13261 ctgtaacatg ccgacaatgg gggtaatacg atcttctggc ggcaaatgct gcaggagttc
    13321 ccccaccaca aaccactggc gggcaaaaat taatgaactg aacggcagct cacggattaa
    13381 cttatcgcca tcatcaggtt gataacattc ataaatgaca taacccgcat tctctttcac
    13441 gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg gtaatttctg cggcgcactc
    13501 tttttcaaag cccggacggc acagcaatac aaccttattc atgacttacg cccttgcgct
    13561 ttaaacggat agcaccaact aacattaacg cccagcccgc aaggaagctc acgccgccaa
    13621 ccggtgtgac aaacgcccac aaacgcagat gggacagcgc caggcaataa aggctgccgc
    13681 tgaacagcac cgtgcctaac gcgaggaaaa cgctactcca gtaaaaccag atactgatgc
    13741 gacgctgcat tgccaccgcc agacctaaga tcgccagcgt atgaaacgcc tggtattcga
    13801 gaccggtttg gatccagccc atctcaacgg cccccatggt tttacttaac acatgcgcac
    13861 caaaagcgcc cagtgccaca aaaatgaagc cgctaatagc ggcgaaaatc agcataaaac
    13921 ggctggtcat ggtcgtaccc tacgtaaatt attgttcata acgaaagcgg aatttttctt
    13981 gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc ggctatttta cccagttctg
    14041 cctggctgtc atgacaaacc agataaaaag catttttact gaccagaaca tcattaaacg
    14101 ggcaaacaag acgtccggcc tcgatttcag attgcgccat cacgttattt gccagcgcca
    14161 ctccctgccc gtgaatagcc gcttgcagca ccatggcgct atgactaaaa attggccctt
    14221 gctgaacgtt gatatgatta aaccccaact gtcgggtata tgtctgccag tcacggcgtg
    14281 acgcatcatg taataacgta tgtttagcca gatcttccgg ggtcttcaag ggtttttcgc
    14341 ctgtcagcag tagcggcgaa cacaccggca ataaatattc ggcgtacagt ttttccaccc
    14401 gtagccccgg ccagttgccc cgaccataaa atatcgccac atcaacatca tccgccagct
    14461 tatcttcctg acgatcaacc gcctggattc gaacgtcaat tcccggataa gctgaattaa
    14521 agctggaaag tcgcggaacc aaccaatgaa tggcgaaact ggggagtaaa ctgaccgtca
    14581 acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc ttcggttaat tgcgaaaata
    14641 tctctttgat atcgaggaaa tagctttgcc cttcctcggt cagcaggagt gaacgattac
    14701 ggcggcggaa cagttttagc cccaaaaaat cctcaagaga cttgatttga tgacttactg
    14761 cggcctgggt cacaaaaagc tcttctgctg cgcgagtaaa acttaaatgg cgtgctgcgg
    14821 catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt agacatggct tttaaacttt
    14881 gatgttaaat gaatttaaca attagatcac actgtgtaac ctattagttt ttttaatctg
    14941 agccattata aattgtccgt tgagcttcta ccagcaaata cctatagtgg cggcacttcc
    15001 tgagccggaa cgaaaagttt tatcggaatg cgtattctgg tgaacttttg gcttacggtt
    15061 gtgatgttgt gttgttgtgt ttgcaattgg tctgcgattc agaccatggt agcaaagcta
    15121 ccttttttca cttcctgtac atttaccctg tctgtccata gtgattaatg tagcaccgcc
    15181 taattgcggt gctttttttt accttgcgat cgcgaattac tgttccagtt cgaccatctc
    15241 tttcacgtca gtacggttga tctgctgttt gttgccatta gcgtctttat acgaaatcat
    15301 accgatatcg ttatcagtct gtggtttgcc gtcagagacg atggtacgtc cgtcattggt
    15361 gtgcatcaca tagttcgaac cggaacaggc gctcagggca aaagtcagca tacaggcaga
    15421 aataattgcg gcagtctttt tcatcatctt ctccttaaaa cgattaataa atcttaatag
    15481 tattgattaa caggctaaaa ttaacgccta acactattca gcatatgtta cttacgcggc
    15541 ttcgccagga tatccagata attctgatgg ttagcactct ccttgtatca aagtgaattt
    15601 tgcgtcacga tcggtgcatc aagccgagga gtaccatgaa cgtttttaat cccgcgcagt
    15661 ttcgcgccca gtttcccgca ctacaggatg cgggcgtcta tctcgacagc gccgcgaccg
    15721 cgcttaaacc tgaagccgtg gttgaagcca ctcgacagtt ttacagcctg agcgccggaa
    15781 acgtccatcg cagccagttt gccgaagccc aacgcctgac cgcgcgttat gaagctgcac
    15841 gagagaaagt ggcgcaatta ctgaatgcac cggatgataa aactatcgtc tggacgcgcg
    15901 gcaccactga atccatcaac atggtggcac aatgctatgc gcgtccgcgt ctgcaaccgg
    15961 gcgatgagat tattgtcagc gtggcagaac accacgctaa cctcgtcccc tggctgatgg
    16021 tcgcccaaca aactagagcc aaagtggtga aattgccgct taatgcgcag cgactgccgg
    16081 atgtcgattt gttgccagag ctgattactc cccgtagtcg gattctggcg ttgggtcaga
    16141 tgtcgaacgt gactggcggt tgcccggatc tggcgcgagc gattaccttt gctcattcag
    16201 ccgggatggt ggtgatggtt gatggtgctc agggggcggt gcatttcccc gcggatgttc
    16261 agcaactgga tatcgatttc tatgctttct ctggtcacaa actgtatggc ccgacgggta
    16321 tcggcgtgct gtatggcaaa tcagaactgc tggaagcgat gtcgccctgg ctgggtggcg
    16381 gcaaaatgat tcacgaagtg agttttgacg gtttcaccac tcaatctgcg ccgtggaaac
    16441 tggaagcagg aacgccaaat gtcgctggcg tcataggatt aagcgcggcg ctggaatggc
    16501 tggcagatta cgatatcaac caggccgaaa gctggagccg tagcttagca acgcttgcag
    16561 aagaagcgct ggcgaaacgt ccaggctttc gttcattccg ctgccaggat tccagcctgc
    16621 tggcctttga ttttgctggc gttcatcaca gcgatatggt gacgctactg gcggagtacg
    16681 gtattgcctt gcgggccggg caacattgcg ctcagccgct actggcagaa ttaggcgtaa
    16741 ccggcacact gcgcgcctca tttgcgccat ataatacaaa gagtgatgtg gatgcgctgg
    16801 tgaatgccgt tgaccgcgcg ctggaattat tggtggatta atgacaaacc cgcaattcgc
    16861 cggacatccg ttcggcacaa ccgtaaccgc agaaacgtta cgcaatacct tcgcaccgtt
    16921 gtcgcaatgg gaagataaat atcgccagtt gatcatgctg gggaaacagc ttccggcttt
    16981 gccagacgag ttaaaagcgc aggctaaaga gattgccgga tgcgaaaacc gcgtctggct
    17041 gggatatacc gtggctgaaa acggcaaaat gcacttcttt ggcaacagcg aagggcgcat
    17101 tgtgcgcggc ctgttggcgg tgttgttgac tgccgttgag gggaaaaccg ccgccgaatt
    17161 acaggcacag tcgccgctgg cattgtttga tgagctggga ttacgtgcgc agcttagcgc
    17221 ctcacgcagc caggggttaa atgcgttaag cgaggcgatt atcgctgcga cgaagcaggt
    17281 ttaagcacag aaccgggtcg gataagacgt tcgcgtctca tccgacctga ttgtttaacc
    17341 ctgacgcgcc gctttcgcca tcatcttctt cagcgcatga gaaaccgcaa caaacccaaa
    17401 ggtggcggtc accatcgttg ccgcgccaaa tccagatgca caatccatcc gcttcggccc
    17461 ttctgccgtg gctttcatcg cacacaccgt accgtctgac tgcgggtaca ccagcgcttc
    17521 agtagaaaac acgcaatcca cgccgagctt acctttactg tttttcacta cgccaaaatc
    17581 gcttttcagg cgctcgcgca acttcgccgc cagcggatcc tgaatcgttt tcgccagatc
    17641 ggtaacctga atctgcgtcg ggtcaatctg cccacccgcg ccaccggtcg tgaccagcgg
    17701 gattttattg cgccgacaat aagcaatcag cgccgctttg ggccgtacac tatcaatggc
    17761 atcaatcacg tacgaataac cggcactcat atattgcgct acgttatccg gcgtcacgaa
    17821 atcatccacc accgttacac ggcactccgg gttaatctgg cgaatgcgct ccgccatgac
    17881 ttccgctttt gccagcccga cgttatcgcg cagagcgtga atttgccgat tggtattggt
    17941 gacgcacaca tcatccatat cgataagcgt gattgcgcca atccccgtgc gcgccagcgc
    18001 ttccgccgcc caggaaccga caccaccgat accaaccaca caaatatgcg cgtcagcaaa
    18061 cagttgcaac gctttttcac catacagacg cgctgtgcca ccaaaacgct gacgccatgc
    18121 atcactaatt accacagaca tagcacctca gaattaaaaa gggcgaggtt atccctcacc
    18181 ctaaacgcat aaatgcctga tgcgctacgc ttatcaggcc tacataactc ctgcaatatt
    18241 ttggatttgc atgatttgta ggccggataa ggcgttcacg ccgcatccgg tatgaacaac
    18301 gcgcacttag tcaataatca ggatgtgagg ttacccctca ccctgtcata tccgtaaaaa
    18361 cggcatacag aatatcacat cagccgctaa agacgttacc tgcgcccgga gccgttttca
    18421 gcacccaaac ccgtccatag tggttgtacc aacctgcgcg gtgtccggct tccggcccga
    18481 tcccttgata gatatcgaag tgttggcctt tgattgcgcc cccgacatcc aacgccacca
    18541 tcagacgcaa ttcgtactga ccgttaaatt tgccgttatt atccagcagc ggcacttctg
    18601 ccagcaaggt agtacctggc ggaataattg aacgatcgga ggcaacagaa gcacggccaa
    18661 ccagcggcac cgcacttgcc cctttcaccg gtgcaaaaga ttgcggttta aagaagacga
    18721 aagacgggtt ctgttccagc agctcgcgaa cctcggcttc actgtgtgtt tcgccccaat
    18781 gacgaatcgc ctgcatcgac atatcttctt ttttcacttc gccacggtcg atcagcacct
    18841 taccaatgct gcgataggca tgaccgtttt tccctgcata gctgaaaaag ttaagcggac
    18901 tgccatcacc aaagtcgata tacccactac cctgcacatc cataatgaag ttatccatca
    18961 gagagttgct gtaagcgagg atgtatttat cgcccaatgc cccggcgtag atctccgcac
    19021 gagacggcag acgaccacgt tttggcggca tacggtaaat aggatactgg aactcgccct
    19081 ggcgggtatg gcgcgcctga attaccggcg tgtaatagcc tgtaaactgc acgttaccat
    19141 agttgtcggc accttccatc tgccaggcat caatgccgaa ctggcgcata ttgcgggtat
    19201 caccgcctgc gcgcagccac tcttgcaccg cgttataaac attactctgg ttgccataca
    19261 gacgcggtga cgaattacgg atatggttaa tttgctcggc aaaatcaccg gcgttaatcg
    19321 gcgcgccaac ggcatctggc tggttcacca gagagaaagg ctgggtaaat ttcccgtctt
    19381 tatattgctg tccgcgatcg gttggtttgg aagagcaggc ggcaagcatt gccacaaccg
    19441 tgcccataag aaggtacttt acccaacgtc ctttcattgt tctcttcttc aggttaaaaa
    19501 ataaggcgca acgaagataa caaaccgccg cgtttaatga aatgagcagc ctctccctga
    19561 cgcaaatttt gcacaaaaaa taggctttag tggtttgttt ttgttcaaaa tcatgctaaa
    19621 tccgtgatcg gggtaaaaaa aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc
    19681 acggacgcgg ggtggagcag cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt
    19741 tcaaatccgg cccccgcaac caattattga acaccctaac gggtgttttt ttgtttctga
    19801 tctcccataa aaaaagcgcc attcagcgcc tttttatcat ccccttctcg ccagcgttgc
    19861 cccatcggca aaatacgctt taatccccgc aagaatagac tccgcaactt cctgctggaa
    19921 agtcgccgtt ttcagtttac gctcttcctc aacgttactg ataaacgccg tttcgaccag
    19981 aatggaggga atatctggtg cctttagtac ggcaaacccg gcctgttcaa cttgattttt
    20041 atgcagcttg ttgatttttc cgagcttatt cagtaccgct ttaccaaact taaggctgtc
    20101 agcaatagtc agcgactgca ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct
    20161 tttgctcacg ccaccaatca agtccgaggc gttctgggtt tgtgccagat attttgccgc
    20221 agtactggtt gcgcctttgg ttgagagcgc aaacaccgag gaaccgctcg gctgacgact
    20281 ggtaaaggcg tcggcatgga tagagacaaa caggtcagca cgctgtttct gggcttttgc
    20341 tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt
    20401 gccctcttcc tcgatcagag agcgcaaacg gcgagctatt tgcaatacca catctttttc
    20461 gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat
    20521 aatgacaatc ggacgatcgc gccctgcttt acccggttgt ggaccacttt gcgctggcgg
    20581 cacctgcttt tcgaggtcgc ctttgttgta atcctccagc agcgccagca gcgggtcctg
    20641 catatcctgt gcattggccg gatagaggtc catcaccaga cgctctttaa acccggcgac
    20701 cggcgcaagg gcaaacagct gcggttttac gttttgcttt aattcaaaaa ccatgcgtac
    20761 ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat
    20821 ttgtgccgcc atccccttga gtaccgagtt caggtttaca tcttcgatat ccaccaccac
    20881 gcgttcaggg ttactcaacg cgaactgctt atatttcagc tgacgatttg attctaccgt
    20941 cacgcgggtg tagctggacg caggccagac gcgcaccgcc acgacctggc taaccgcagc
    21001 caggctgacc tgacttacgc tcaatagcca catagcgccc gcgccttgca gtaaacgacg
    21061 acggctgatt gcagtgttgg atcctgacat gcctctcccg agcaaaagaa atctaagctg
    21121 tgtaacaagt aaacgactaa tttgaccgga aactttagcg aaagacgcat aatctgtcat
    21181 ctaataaacg gtaaacattc tttttatatt cacggcatta ctgataaaaa agtcgctctc
    21241 gcataaaatt tacacttgca ccctgcgaaa aaacagaata aaaatacaat aatttcaaat
    21301 aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc
    21361 cgccattcgg ttccctatat caatacccac cggggaaaaa cgtttgtcat catgctcggc
    21421 ggtgaagcca ttgagcatga gaatttctcc agtatcgtta atgatatcgg gttgttgcac
    21481 agcctcggca tccgtctggt ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg
    21541 gctgcgcatc accacgaacc gctgtatcac aagaatatac gtgtgaccga cgccaaaaca
    21601 ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg atattactgc tcgcctgtca
    21661 atgagtctca ataacacgcc gctgcagggc gcgcatatca acgtcgttag tggcaatttt
    21721 attattgccc agccgctggg cgtcgatgac ggcgtggatt actgccatag cgggcgtatc
    21781 cggcggattg atgaagacgc gctccatcgt caactggaaa gcggtgcaat agtgctaatg
    21841 gggccggtcg ctgtttcagt cactggcgag agctttaatc tgacctcgga agagattgcc
    21901 actcaactag ccatcaaact gaaagctgaa aagatgattg gtttttgctc ttcccagggc
    21961 gttactaatg acgacggtga tattgtctcc gaacttttcc ctaacgaagc gcaagcacgg
    22021 gtagaagccc aggaagagaa aggcgattac aactccggta cggtgcgctt tttgcgtggc
    22081 gcagtgaaag cctgccgcag cggcgtgcgc cgctgtcatt taatcagtta tcaggaagat
    22141 ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg gtacgcagat tgtgatggaa
    22201 agcgccgagc agattcgtcg cgcaacaatc aacgatattg gcggtattct ggagttgatt
    22261 cgcccgctgg agcagcaagg tattctggtg cgccgttctc gcgagcagct ggagatggaa
    22321 atcgacaaat tcaccattat tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat
    22381 ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc
    22441 agttcatcac ggggcgaggt tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc
    22501 ttaagcaaat tgtttgtgct gaccacgcgc agtcttcact ggttccagga acgtggattt
    22561 accccggtgg atattgattt actgcccgag agcaaaaagc agttgtacaa ctaccagcgt
    22621 aaatccaaag tgttgatggc ggatttaggg taagcgatga aaatcaccgg atgcggcagg
    22681 cgtaacactc gtgcgtcgca tccggcaatt acgtttattc ccgcgaacta aacaacgccg
    22741 ccagaccact gcgccgctca gtacgagtgg cgattgccgc acttaatata cgctcatcgg
    22801 catacagcga cagacgacga cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta
    22861 ctaccggcgt gcgttggctc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg
    22921 atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg caagcgactc ggttgcacag
    22981 acttaatatt gccgtccggc atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg
    23041 caataccgat atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg
    23101 gtcggccttc gtaccaacga gagtgcggat gacgatgaat tttgcgcttc tgttgcataa
    23161 actgttcaat tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca
    23221 aaagctggta ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca
    23281 gatccagata acgtccgtaa cccgcaagag cttcctcaag catcgcaata taatcttcgc
    23341 cgctctgtaa aagtcgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg
    23401 ccgttttatc accacggttg atcgccgcag ctaactgacc aatgccagaa tcgctgccga
    23461 aacgatagct tttttgcagc aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg
    23521 ttcctgccgg aacgtgagtc cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa
    23581 agcccgcgtt ggcataagcg cagatatcgc ccagcacagc cccagcctca accgaggcca
    23641 gttgatcacg atcgccgaga aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc
    23701 tcgacatcat aggcagatcg atcattgacg cttcatctac caccagcaca tcaagatgca
    23761 gcgggttacc ggcatgatga cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt
    23821 gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc
    23881 gcaaagcctt gccgagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca
    23941 gacggatacg gcagcgttcg ccgtcggcca tttgaattaa cgctgccagc aacttcgcca
    24001 cggtggtcgt tttaccggtg cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg
    24061 ccactgccgc tgcaactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca
    24121 gggtttgcgc cagtagagct tcatcaacct caatggtatg attcacctca ttgaagaagc
    24181 gcgctaccgt ccgctcgtta caccacatgc gattcaaata aagacgatcg ccacagagga
    24241 tcatcggcgt gggttcatct cccctgctga ccgcttgaga agccagcaag cattcttccc
    24301 aattttgtag ctcaccgatt tcactgacac aggtcgccaa cagcggattc gacgcctcgt
    24361 tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc tccggcatca tgacttaaca
    24421 gtgccgccgc gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaattgca
    24481 catccagcgg gcgtagctgt ttgtgctcca cagcttccag taattgcttt tgcaatttca
    24541 ttacgcctcc tccagggtca taccggcaaa catctcatcc atcagggcaa tcaacccggc
    24601 gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct ttatcaacgc cacgcaggaa
    24661 cagatagata acgccgccaa agtgaagatc atagtcgtaa tcagcaatgc gatggcgcag
    24721 ataacgatgc agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat
    24781 cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct tcacccagcc agttggattt
    24841 atagtcgagc aggtaataac gcccttcgtg gcggaatacc aggtcgataa agccttttaa
    24901 catgccgcgt acctgcatga actccagcgg cgggcagcca gcggatagcg ggtcaaactg
    24961 gcgaattagt gcatcaagct ggctggcgat gagcggttcg ctaatcggca ggtaaaactc
    25021 catctccacc tgtttatcgc ggtcggaaag ctgactcagg ctaacacccg tttcattgag
    25081 aggtgcctgg aggacagccg tgatccactc ggtcaacacc ggttcccact gcgattcaaa
    25141 gccgccgagt tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag
    25201 gtcttcaaac aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg
    25261 tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg
    25321 catcagatcc tgagcgatac cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg
    25381 ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc agttctgcag ttaaagcatc
    25441 attaacctgc cagggctggt tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc
    25501 gcataacgct tcaatacagg tgcgaagccc tgccgcatct tgcggttccc ctttttgcag
    25561 caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg
    25621 caccagcggt gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag
    25681 caaacgcaga tcttccgcca gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc
    25741 attaagatcc aaaactgcct caaacgagtg gcgatcgtga taaaacgcct gatcctggac
    25801 gcggaaattg gtaataaatg gcaaccagac cagcggatat tccagccctt tcgatttatg
    25861 gatcgtgaca atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc
    25921 attactgtct ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc
    25981 cagctgcgtt ccggcttctt gaagcagttc gctgatatgc aagatatcgg taagacgccg
    26041 ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg
    26101 cagcatcggc ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac
    26161 taccgcatcc cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc
    26221 catcattgac gttgccagcg cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc
    26281 ctgcaacaac caaagcattt cctgcgcttc cagagtttca aaaacactgt cgcggttcga
    26341 aaggtaaacg gaagggattt ccagcaacgt taaggcatcg cgcacctggg cggcctcctg
    26401 gcggctgcgc accagcacgc tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt
    26461 catcagcaac gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca
    26521 tacctgcgcc atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca
    26581 cattttcatc gcaggctgcg tttcaccttt aaatacaaaa cgtaacgcct gatttttccc
    26641 ggctgatttc actggaataa acggtatttc gcgaaacatg aacgtgtcat cagtctggct
    26701 gaaaagcttg ttcacactgt tcaccattcc aggtgcggag cgccagttgg tgtctaaagt
    26761 gtagtgggcg tgaacttcac tacgcgcctt catataagtg aagatatccg caccccggaa
    26821 tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg
    26881 ccagatacgg cgaaaaattc ggtactgctg ggggtcggta tcctgaaatt catcgatcat
    26941 tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag
    27001 cgcggaatcg agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc
    27061 acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg atcaacagat cgcggatcga
    27121 caatggttct gcaagcagtt gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc
    27181 cccggccttc gtgcgatctt ctaagaaacg ctgggagaat ttttccagcg actccggcaa
    27241 ctgataactg tttgtctctt cttctgccca ggcgctgatc ttttcgatcc atttagcctg
    27301 attgctacgg ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc
    27361 acccactgcg tcgcgccact gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg
    27421 gcgggaagcc agcgtttcat catcgggcgg cggtgctttg ataaccggcg gttcgccttg
    27481 cagataacga ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagacgac
    27541 ctgggctatt tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg
    27601 gtagcgtagc agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa
    27661 ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc
    27721 catctgccgt tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcttc
    27781 cagcaggcgt tcgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc
    27841 gtggatattg ctacggatac gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac
    27901 caccagcagt tcttcaacga tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag
    27961 taacaggcgc aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc
    28021 aatcaggcgc tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact
    28081 cattcttttc actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt
    28141 tccagccttc aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca
    28201 gaatagccat gccttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg
    28261 gcgtcagcag ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc
    28321 gatcgaaatc tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca
    28381 tctgggtaat taccgcctgc tggatttgcg caaactcatc tggcttcatc gctcgcaatt
    28441 ttgcctctgc ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat
    28501 cattgctttg caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa
    28561 acacggcata gcccaattgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga
    28621 tctgccccaa cagagagcta taggctgagc tggtgtattc atcgtagcca gtcggtacaa
    28681 ataccgctgc cagtgcggag tcggtgctgt taccggcttt ttcaaagatg acggattgtt
    28741 ttttatcgac cactacatct ttgtttcgac accactctga accatcagcg cccaactgtt
    28801 tttgcacatc gcgtgccagc gttgttgcct gggcctcggt catgttgccg ataaccataa
    28861 actctggtcg agcccctgat tttaaggcgt cgcgataggc cagcacctct ttcaacgtaa
    28921 tggagggcaa aattttacgc cgttcatctc gcgagaagta cggcacttgc gagagcatct
    28981 gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc tgcggaatcc atcatctggt
    29041 tataccagga cttcgcctgc tcaagctgct cttccgtagc ggtatagcta aagtacccct
    29101 cgagcaatgc ctggaacagc tgcggcagac gctgggtgta accattagca ttaatcataa
    29161 ggccgttgtt agcgttggtg gaaaaactta tgccaccaac cgacgcctgg ttgcttaact
    29221 gatcaagcgc cagccctgcg agataatcat tgagcgcaaa catcacctga ttgcgggcgc
    29281 tgtccatggc tttcggatta cgcaaaatca ggctgacatc agctttgggc tcgctggcaa
    29341 aataacggct tggcgcatac accacgcgca gattcgactc atcaacaatc agctctggat
    29401 ggtcgtattt cttctctgac ttaatcagcg agaaatcatc aggaatataa gggttaagct
    29461 ctggcaaaga gagcgcaatg tcggcggctt ttttctgcca gtcggcgaaa gtttgtgcgc
    29521 tgattttatc gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt
    29581 tcgggctgat ataccagata cgcgcattct gcggcgtcat catcgccaga cgttccttta
    29641 ctgctttagc atcgtaccga tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa
    29701 cgcgaatcat ggtatctgcc agccattcga cgtaatccat atcacgggtg atcgacggat
    29761 aacggaagtc gatatccagc acattcgcca gttcatcgaa gtattgttta tcaatgcctt
    29821 tttcacgtaa cagattgaga tagctaaaaa ttgccgccac aacctgatcg cgattagcca
    29881 agcctttatc agttaaagac gcagagatcg ctaatacgcc gctgttgccg ttgacgatag
    29941 gatcggagtt ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg
    30001 tacctgggct gcgattgcca atcagatagg taatcaattc atcggtttta ctacggaact
    30061 tcgctgagtt gttatcgatg cgaaactcaa cgcgcaacac tttacgcggc agcgcaggga
    30121 cgtaatgaat gataatgccc ttttgcgcgt cggtgactac cggcacggtg atttccggtt
    30181 ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc cgccattttt gccaactccg
    30241 gcagcggttt attactgtaa ataaccgcct tcatcaaatt ggcggaatag tacttctcgt
    30301 ggaaatcttt cagcgcctgc tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt
    30361 taccaccaga aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg
    30421 ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc agcgttcact gcattacgct
    30481 cacgttcggc gtatttcttg tcgagcaagg gttctgcaat agcatcggcc aggcggtcta
    30541 ccgcaccggg caaggcgtcg ttctcaactt ccagatagaa agccgtgcga tacggcgcag
    30601 tgctggcatt gtgactaccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg
    30661 ggtacttttt cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg
    30721 cctcgggatc ttccagcgac ccaacgggca ctaccagcgc cgagagcgat ttaactgcct
    30781 gcggatcaga aaccagcaag accaccatac cgttatccag acgtatagcc tgatactggc
    30841 ggttatcttt atcactttta cggatggttt cctgaatcgg ctgccatccc gtttctgcct
    30901 gacttaatgg tgcccagagg gcaacaaaca acaataatgc tttgaaccag gtgctgcggg
    30961 gcattcacgg acctcataat caactttatt ttctggacag attcaacagg ttaacgcaaa
    31021 tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc tatgtttata taaccatcag
    31081 tccgtgactg gtgcgcatca taaagtaagc ggatagattg cgcaattttt atacaacact
    31141 catgactgat taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatggcc
    31201 tccattgtct ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg
    31261 ccacgcacca tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac
    31321 gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg
    31381 ccgccacttt caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc
    31441 agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcaccg
    31501 tctttgcgta gaaaaaggcg actttcgcca ttaccaccgc tggcacagta gacaaggtgt
    31561 tccagccaaa gttgcattcc ctgcgccaca cttaataaag agggacgcca gcgcaacagg
    31621 ccatccggct gcacctgcgg caaccagcca gttatctgca caccgttgca ggcgagatca
    31681 atttccatac tctgccccgg ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc
    31741 tcctggcact gtgtttccca gaaaatttca ccaaaagcgc catacggtaa atcccctgcc
    31801 gcccggaagc ggcggaacaa gcgttcggca tcatcctgct caaccagtgc attcaataac
    31861 tgctgattga tttgataacg gctaagtcct tccagaataa atggctcggt gtctgggatt
    31921 tcgctgtctt cagtatgaaa gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga
    31981 tgtgcccaga atcgttgtag cgtttccagc ggcacggttt ccggtaaggt aaacggcagc
    32041 ggctgaacaa attcagaatg tgctttacca gcctggctgg ccgcaggtag ccattcacga
    32101 gcatagcttt gtcgttcgcc tggctggtag ttttgcggat caaacggcat ccgggtatgg
    32161 aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg
    32221 cccggtagat aatgactttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg
    32281 aaacgttcac tgttatcctg aatggaacgc ccgatatagc tgatatagag tttttgctgc
    32341 gcggaaatta acgcttccag gaacaggtag cggtcgtcat cgcgacggct acggtcgcca
    32401 cgcttcggtt tctggctcat caggtcaaag cccaatggcg caagctgacg tggataaacg
    32461 ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga
    32521 gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga tacgttcttg atccagacgc
    32581 tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa
    32641 ccttcggcga taatcgcctg ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc
    32701 gcatccggca ggaagaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttctagc
    32761 ggacgctcct gtgccagccc gcgacgccag atgtttagct gcattagcag tgaagccaga
    32821 tgccccacca gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac
    32881 tcgccctgcg cgctctccat cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag
    32941 gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa
    33001 cgaatgccgg attcgttgac ccactggcgt aaataacgca gcccttcttc ggtgatgtca
    33061 aaccgcgccg ccagcaccgg cacatccagc agcgccagca catcctctga cacaaagcga
    33121 ctgtcaggca gtgataacag gctgataaac gcttccagta ccggatgtga ctgccgcgca
    33181 cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc
    33241 tgaataaacg gactgtagct gtcgatatca gccaccatca cgatgatgtc gcgcggagta
    33301 agtgtcgggg cttcctccag catcgccagc aagcgatcgt gtaaaacttc aacttcacgc
    33361 tgcgggctat ggcaaacgtg gaatgtgata ctgctatcca gtggatcaag cgggcgtttg
    33421 ttatcgctac gggaaaactc ttcgatgttc acacccgcaa cggcgcggtt ttccagttcc
    33481 agaatgtcag actgaatgtt atgcagcagg ttatctggcg tcacatcgac aaaagcgtcc
    33541 agctcctggc tgctctccag gtcagaaagg agataaatgt agtcgcgccc aagcttcccc
    33601 catgaggcca gcagcgagtt gccgacatcc tgttcaccat cgctgttaaa gagctgtccg
    33661 gcattttcgc tatcgcgaaa tagcggcaat tcgcgatctt caaaactgtg tcgacgctga
    33721 cgagtcagca gtttcgccag ataagcagga tctttaatat cgccccagta ataacggcag
    33781 gggttggtaa acaggagatg gatttcaata tgtttaccca gcgcctggag cgcctggaga
    33841 taaacaggcg gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg
    33901 caggtcgtcg cggactccag cgtttcgata aagcgctgat agagattcgc gcggtgccag
    33961 cgcggttgtc cgagttcatg ggtatattcc accagcgcct tccacaacgg ggcttgccag
    34021 gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc
    34081 ggacgataga ccagatactg gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt
    34141 tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc
    34201 agcaattgcg gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaggcgctc
    34261 tctttgggga tttccggtaa cacccggacg aacatatccc agataaagct cgctggcagc
    34321 ggaaaatcaa tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgc
    34381 gccataccgg tactttgcac cagaatcatc tctggttcga aaggatcgtc cagccgttcg
    34441 cgttcgacaa taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc
    34501 cttaacatag cggctcctga ctactgacga ttcgggcaat gcaggcgggt catctcgcct
    34561 tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc
    34621 tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc
    34681 caaccatgcc gccagagctg ttggtactgg tttctgctgg cgagactgtt cattgatgtt
    34741 cgctggatac ccgataacgc agtgacaatc atcaccatca gcaccatcag caccatcgca
    34801 accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataatgccc
    34861 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    34921 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcttcat
    34981 tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc gagcactgaa
    35041 ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac tgaacgatgg
    35101 cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    35161 gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    35221 acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    35281 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa
    35341 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    35401 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg
    35461 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    35521 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    35581 ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    35641 tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta aactttcacg
    35701 ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc
    35761 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    35821 tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    35881 tttttgaagc gaatatgccc ggcccatgcg gtattacgca ggccaaagaa agcaagcgaa
    35941 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    36001 ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg
    36061 ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    36121 tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    36181 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    36241 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    36301 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    36361 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    36421 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aacgcccgga
    36481 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    36541 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    36601 tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    36661 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    36721 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    36781 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    36841 acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt
    36901 accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    36961 ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat ggtgacattg
    37021 ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag ttcgtggatg
    37081 atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg caggttaaaa
    37141 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    37201 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    37261 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    37321 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    37381 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    37441 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    37501 accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    37561 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    37621 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    37681 ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    37741 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    37801 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    37861 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    37921 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    37981 cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaacc caccagatac
    38041 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    38101 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    38161 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    38221 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    38281 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatga
    38341 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    38401 aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    38461 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    38521 cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    38581 aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt gcgatcaacg
    38641 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc
    38701 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    38761 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    38821 gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag gttgcccgta
    38881 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    38941 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc
    39001 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    39061 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc
    39121 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc cagtttttct
    39181 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    39241 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    39301 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    39361 ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc gcccatcacg
    39421 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctga gccatctcgc
    39481 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    39541 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    39601 tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta
    39661 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    39721 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    39781 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc ggcaaagcgt
    39841 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    39901 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    39961 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    40021 tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    40081 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    40141 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    40201 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    40261 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    40321 agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa
    40381 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    40441 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    40501 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    40561 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    40621 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    40681 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    40741 gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    40801 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    40861 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    40921 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    40981 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    41041 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    41101 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    41161 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    41221 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    41281 cccggagcat tctgaataag cccgatatag aaattttaaa atattcatta ttagtcaatg
    41341 cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    41401 ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt
    41461 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atgaaaacag
    41521 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    41581 ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc gattaccggc
    41641 agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa gttacactgc
    41701 gaatattcgg cacataatta ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    41761 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    41821 tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg ccagagaatt
    41881 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    41941 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    42001 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggtaata
    42061 gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta ctgtggatac
    42121 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca ccgttgctgt
    42181 ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa ttaatggata
    42241 tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat ttacagataa
    42301 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga
    42361 ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    42421 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    42481 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    42541 ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    42601 tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    42661 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct cttgggttac
    42721 gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgatttgat
    42781 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    42841 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    42901 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    42961 agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc
    43021 cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc
    43081 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    43141 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acttgattcg
    43201 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    43261 cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    43321 cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    43381 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    43441 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    43501 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    43561 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    43621 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    43681 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    43741 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    43801 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    43861 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    43921 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    43981 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    44041 agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccgacca
    44101 actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt tcgctgctgg
    44161 atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    44221 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    44281 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    44341 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    44401 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    44461 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    44521 atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt gtacgctgtc
    44581 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    44641 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtacatc
    44701 aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc gttaaatatt
    44761 aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc gcaccgaagc
    44821 caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa atgcccagca
    44881 tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga
    44941 cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca
    45001 gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggagaaaa
    45061 tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg
    45121 taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcactacc atcgcgttga
    45181 gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca
    45241 ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg
    45301 tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga
    45361 gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg gcaacgaccg
    45421 caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag tcagccagca
    45481 caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc attaaaccgt
    45541 tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg
    45601 aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc
    45661 agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg
    45721 cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca cctacaaaca
    45781 ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt aacgtcagag
    45841 tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt
    45901 tcatcccctt cgaccacaac gaagtgttaa tgtgcactga ctcactcatc gtgttgttcc
    45961 gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc
    46021 atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg
    46081 gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc
    46141 tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca
    46201 agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt
    46261 tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg
    46321 tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca
    46381 cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt
    46441 ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg tagaatacgc
    46501 cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg
    46561 gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta
    46621 ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg
    46681 ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg
    46741 cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata aaggaacact
    46801 tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc
    46861 ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg
    46921 acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc ttcagaaccg
    46981 gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc caggcgcggc
    47041 atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct
    47101 tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta
    47161 tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc
    47221 agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg cgcgatggcc
    47281 ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct
    47341 tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga
    47401 taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac
    47461 attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat acgcgcttcc
    47521 atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca
    47581 tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg gaacaagcga
    47641 cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca
    47701 ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc
    47761 agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc
    47821 accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg aacaaagtcg
    47881 ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac
    47941 actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta
    48001 ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga
    48061 tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg
    48121 attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat
    48181 taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga
    48241 gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacgacg
    48301 actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt
    48361 gtcggttact gttacagatt gatgaccggc aaaaaaaacc tgcgcatctg cgcaggctgg
    48421 tgtaattcat gtgctcaaca cgaagttgac ttcacctatc aatacctctg ggatcaccac
    48481 tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt
    48541 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca
    48601 cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaacaatgta
    48661 agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
    48721 cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcttc
    48781 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
    48841 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
    48901 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
    48961 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
    49021 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc
    49081 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
    49141 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
    49201 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
    49261 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattccgg ccaatgatcg
    49321 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
    49381 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggcgc
    49441 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
    49501 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
    49561 cgtaacgatg gcgacccagg ctgccgaact ggctttggca ctggcggata atcgccctct
    49621 cccggaaatt actaatgtat ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
    49681 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
    49741 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
    49801 gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc
    49861 ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa
    49921 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggc ccggcgacga
    49981 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
    50041 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca
    50101 caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact
    50161 gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag
    50221 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
    50281 caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
    50341 cgaattcgat aacctgacgc accatagcac cacacacctg ctccagatgg gcataatcaa
    50401 cgccagaacc aatgtgcatg tgaatgccga ctagctgcag atgatgacgt tgtatcacgt
    50461 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
    50521 tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc
    50581 ctggcgaaac ctggcccagt tggtcgagcg tatcaacaga acccgcattc accggaattt
    50641 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
    50701 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
    50761 agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac
    50821 aggctttctg tgcaaagcgc accacttcaa actgtttcag cgctgcaatc tgccgacgaa
    50881 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
    50941 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
    51001 gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
    51061 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    51121 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag
    51181 cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg
    51241 tgatcgggct gaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac
    51301 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg
    51361 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    51421 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    51481 taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    51541 atttaggact cactgagaca ctccatacgc ctacgggaac agaacgtacc gaattactct
    51601 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    51661 cgccggatga ttttcagggt gagaattaca tcagcctttc ccgtactgac agctatcgcc
    51721 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    51781 acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtatttcg gtggttaacc
    51841 cgctcaccgc actagattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg
    51901 ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggttcagg
    51961 cgtttagtgg gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt
    52021 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc
    52081 aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa
    52141 aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    52201 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    52261 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    52321 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    52381 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    52441 ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc
    52501 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    52561 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca tttgcgcaga
    52621 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt
    52681 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    52741 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccaat
    52801 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg
    52861 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    52921 ttggtttccg gaatgagcca gaaagtaata cccacaaacg caatgttcag cgcagtgtag
    52981 agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggc cgcgccgata
    53041 atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc
    53101 agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg
    53161 gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg
    53221 ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa
    53281 ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc
    53341 gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    53401 aagcccgcca ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac
    53461 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    53521 ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct
    53581 tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga
    53641 cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc
    53701 agaactgctg gtaaagcaag accccccaac attgcgcgcc agttaccgct ataactgaac
    53761 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    53821 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    53881 gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg
    53941 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    54001 tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg
    54061 agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta
    54121 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    54181 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga acgtggcgtt
    54241 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacattgt
    54301 gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa atatggatta
    54361 aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa gttgaattat
    54421 tgagattatt attaacctcc taattttaaa gcagataaaa ttcataaagt tcattaattg
    54481 ataattgata tggattattt cataaccatg atatggatta tgatgatcta gagatataaa
    54541 aaaccctgcc atgcggcagg gtcataaaag taagaggaat gaattaacgc gccagccaac
    54601 cgccatccac agcaatggca taaccattta cataatctga agcgctggag gcaagaaaca
    54661 ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg cgatcgagaa
    54721 tttccgcgct acgttgttca tctgcccgca gttgttgggt attgttggtc gccatgtaac
    54781 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgtttgcc atcaatcgcg
    54841 tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
    54901 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
    54961 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc
    55021 agtctttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca
    55081 ggatatcaat atgaccaaat tcagctaccg cgcgatccag cagtccagga atgccatcaa
    55141 tctttcgcag atcggcggtc aggcttaaaa aacgacgccc ctgcgccgtg acctgctcga
    55201 tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc
    55261 ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca actttacctt
    55321 ggagagaaaa tgcacttaaa atcataacaa caccttattc gtttatgccc acaactagcg
    55381 caaatcttta acgcccacat ggtccatatc attaaagacc tggttttcac cgaccatgcc
    55441 ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat
    55501 caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc ccatcatgtg
    55561 gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg
    55621 ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact
    55681 caattgacag gtttccagta catccgggac aaaatatttg ttaatcgtgc gacggttact
    55741 ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt ttttggtggg
    55801 atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc gccggagtgg cggtatcaat
    55861 actggcaaag acaacttctt ttgcaccttt accaacatac agggcgtcgc ggtgaccgat
    55921 ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa
    55981 ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga
    56041 aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac
    56101 catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgc gcagcccttg
    56161 ggtatccagc gtttttgcgt gcgcaccgtg gatgctctgt cttacgtcca catcaacctc
    56221 cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta
    56281 taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca atgttttatt
    56341 tttaatttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa
    56401 aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat
    56461 gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca
    56521 atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg gcattacgtt
    56581 tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac
    56641 cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca agcatcttgc
    56701 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
    56761 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
    56821 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
    56881 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
    56941 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
    57001 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
    57061 tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
    57121 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
    57181 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
    57241 catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat
    57301 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
    57361 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagaccg gaaccgcaaa
    57421 cgtcattaat cgtgattgca gaaacgctgt taggaagacc acctttaata gccgattgcc
    57481 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
    57541 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
    57601 cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga
    57661 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
    57721 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
    57781 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
    57841 atgggtagat agggcgttaa tctcccatac ttaacttggt ttatggtaaa ttgccctcca
    57901 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
    57961 actctgtgaa gtttcgcgct ctatggcaca gccgttgatg caggcacgct attccttcct
    58021 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
    58081 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcaaa aacatcagca
    58141 ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg taatctgatt
    58201 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
    58261 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
    58321 gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg tcatgccact
    58381 attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt tctttccatt
    58441 taccttgtcg gtagttggca acctgatcta ttaacaaccc aggtagagtt caatcagaat
    58501 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
    58561 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
    58621 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
    58681 tttagctgcc tgctttctat tccatcttcg tatattgaag cggctaaaga agaaggggtc
    58741 accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc aatttccggg
    58801 attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt
    58861 gaaggtgcca cagaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
    58921 gcatttaacc acgcactatc aattatgttg gtatcgctga ttaccttcat tgtttgttgc
    58981 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
    59041 cttttcatca tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc ctggcgttcc
    59101 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
    59161 taatatccgt tgtggatggt tcaaaattgc tacaattttg aaccattcca tttttcataa
    59221 tattcatttg ttattattcc gccctatcac ttctctcgtc atatcctaga attatcagtt
    59281 agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat gcataactta
    59341 gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata ttctcatacc
    59401 atgagatagt tatctggacc acgttcgacc atgcaagatt aaatcagtga aacataatca
    59461 tatttaataa cgagaaatgc atttttaaat atcttaatca gaattatagc aaatacagat
    59521 taatcgcaat ttttcccagc aaaattacaa aatcaaggtg tattgacatc atcaatcacc
    59581 aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt
    59641 tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca
    59701 ccgcgtttgc agtactgcgc aaactcgt
//
LOCUS       DAJTNV010000012        58049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNV010000012 DAJTNV010000000
VERSION     DAJTNV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(24..278)
                     /locus_tag="OO199_000726"
     CDS             complement(24..278)
                     /locus_tag="OO199_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5534883.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO199_000727"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO199_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5534884.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO199_000728"
     CDS             1228..2694
                     /locus_tag="OO199_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5534885.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO199_000729"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO199_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5534886.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO199_000730"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO199_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5534887.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO199_000731"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO199_000731"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5534888.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO199_000732"
     CDS             5709..7265
                     /locus_tag="OO199_000732"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5534889.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO199_000733"
     CDS             7347..9161
                     /locus_tag="OO199_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5534890.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO199_000734"
     CDS             9162..10256
                     /locus_tag="OO199_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5534891.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO199_000735"
     CDS             10256..11281
                     /locus_tag="OO199_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5534892.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO199_000736"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO199_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5534893.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO199_000737"
     CDS             complement(12876..13220)
                     /locus_tag="OO199_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534894.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO199_000738"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO199_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5534895.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO199_000739"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO199_000739"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5534896.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO199_000740"
     CDS             15616..17376
                     /locus_tag="OO199_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5534897.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO199_000741"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO199_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5534898.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO199_000742"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO199_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5534899.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO199_000743"
     CDS             19113..19340
                     /locus_tag="OO199_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5534900.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO199_000744"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO199_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5534901.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO199_000745"
     tRNA            21195..21271
                     /locus_tag="OO199_000745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO199_000746"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO199_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5534902.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO199_000747"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO199_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5534903.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO199_000748"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO199_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5534904.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO199_000749"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO199_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5534905.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO199_000750"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO199_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5534906.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO199_000751"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO199_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5534907.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO199_000752"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO199_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5534908.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO199_000753"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO199_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5534909.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO199_000754"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO199_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5534910.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO199_000755"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO199_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5534911.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO199_000756"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO199_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5534912.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO199_000757"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO199_000757"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5534913.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO199_000758"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO199_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5534914.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO199_000759"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO199_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5534915.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO199_000760"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO199_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5534916.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO199_000761"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO199_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5534917.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO199_000762"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO199_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5534918.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO199_000763"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO199_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5534919.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="OO199_000764"
     CDS             complement(38328..39347)
                     /locus_tag="OO199_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5534920.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="OO199_000765"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="OO199_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OO199_000766"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OO199_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5534921.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OO199_000767"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OO199_000767"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5534922.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OO199_000768"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OO199_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5534923.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OO199_000769"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OO199_000769"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5534924.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OO199_000770"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OO199_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5534925.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="OO199_000771"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="OO199_000771"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5534926.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="OO199_000772"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="OO199_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5534927.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OO199_000773"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OO199_000773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5534928.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="OO199_000774"
     CDS             complement(53354..54130)
                     /locus_tag="OO199_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5534929.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="OO199_000775"
     CDS             complement(54135..55784)
                     /locus_tag="OO199_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5534930.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     AKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGKTGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="OO199_000776"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="OO199_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58049)
                     /locus_tag="OO199_000777"
     CDS             complement(56503..>58049)
                     /locus_tag="OO199_000777"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5534931.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14203 a        15655 c        14263 g        13928 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ctgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 agcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatca
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accacaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaagaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtcttg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gccacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaatt
//
LOCUS       DAJTNV010000013        58018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNV010000013 DAJTNV010000000
VERSION     DAJTNV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..1102)
                     /locus_tag="OO199_000778"
     CDS             complement(<1..1102)
                     /locus_tag="OO199_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5534932.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1314..1661)
                     /gene="pspE"
                     /locus_tag="OO199_000779"
     CDS             complement(1314..1661)
                     /gene="pspE"
                     /locus_tag="OO199_000779"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5534933.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1704..1925)
                     /gene="pspD"
                     /locus_tag="OO199_000780"
     CDS             complement(1704..1925)
                     /gene="pspD"
                     /locus_tag="OO199_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5534934.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1934..2293)
                     /gene="pspC"
                     /locus_tag="OO199_000781"
     CDS             complement(1934..2293)
                     /gene="pspC"
                     /locus_tag="OO199_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5534935.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2293..2517)
                     /gene="pspB"
                     /locus_tag="OO199_000782"
     CDS             complement(2293..2517)
                     /gene="pspB"
                     /locus_tag="OO199_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5534936.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2571..3239)
                     /gene="pspA"
                     /locus_tag="OO199_000783"
     CDS             complement(2571..3239)
                     /gene="pspA"
                     /locus_tag="OO199_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5534937.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3406..4383
                     /gene="pspF"
                     /locus_tag="OO199_000784"
     CDS             3406..4383
                     /gene="pspF"
                     /locus_tag="OO199_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5534938.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4503..5768)
                     /gene="puuE"
                     /locus_tag="OO199_000785"
     CDS             complement(4503..5768)
                     /gene="puuE"
                     /locus_tag="OO199_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5534939.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5806..7086)
                     /gene="puuB"
                     /locus_tag="OO199_000786"
     CDS             complement(5806..7086)
                     /gene="puuB"
                     /locus_tag="OO199_000786"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5534940.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7088..8575)
                     /gene="puuC"
                     /locus_tag="OO199_000787"
     CDS             complement(7088..8575)
                     /gene="puuC"
                     /locus_tag="OO199_000787"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5534941.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8725..9282)
                     /gene="puuR"
                     /locus_tag="OO199_000788"
     CDS             complement(8725..9282)
                     /gene="puuR"
                     /locus_tag="OO199_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5534942.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9309..10073)
                     /gene="puuD"
                     /locus_tag="OO199_000789"
     CDS             complement(9309..10073)
                     /gene="puuD"
                     /locus_tag="OO199_000789"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5534943.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10285..11703
                     /gene="puuA"
                     /locus_tag="OO199_000790"
     CDS             10285..11703
                     /gene="puuA"
                     /locus_tag="OO199_000790"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5534944.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11852..12016
                     /locus_tag="OO199_000791"
     CDS             11852..12016
                     /locus_tag="OO199_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534945.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12006..13391
                     /gene="puuP"
                     /locus_tag="OO199_000792"
     CDS             12006..13391
                     /gene="puuP"
                     /locus_tag="OO199_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5534946.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13525..13770
                     /locus_tag="OO199_000793"
     CDS             13525..13770
                     /locus_tag="OO199_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5534947.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14083..15726
                     /gene="sapA"
                     /locus_tag="OO199_000794"
     CDS             14083..15726
                     /gene="sapA"
                     /locus_tag="OO199_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5534948.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15723..16688
                     /gene="sapB"
                     /locus_tag="OO199_000795"
     CDS             15723..16688
                     /gene="sapB"
                     /locus_tag="OO199_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5534949.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16675..17565
                     /gene="sapC"
                     /locus_tag="OO199_000796"
     CDS             16675..17565
                     /gene="sapC"
                     /locus_tag="OO199_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5534950.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17565..18557
                     /gene="sapD"
                     /locus_tag="OO199_000797"
     CDS             17565..18557
                     /gene="sapD"
                     /locus_tag="OO199_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5534951.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18559..19365
                     /gene="sapF"
                     /locus_tag="OO199_000798"
     CDS             18559..19365
                     /gene="sapF"
                     /locus_tag="OO199_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5534952.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19433..19786
                     /locus_tag="OO199_000799"
     CDS             19433..19786
                     /locus_tag="OO199_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5534953.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20154..20942
                     /gene="fabI"
                     /locus_tag="OO199_000800"
     CDS             20154..20942
                     /gene="fabI"
                     /locus_tag="OO199_000800"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5534954.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21086..22213
                     /locus_tag="OO199_000801"
     CDS             21086..22213
                     /locus_tag="OO199_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5534955.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22281..24215
                     /gene="rnb"
                     /locus_tag="OO199_000802"
     CDS             22281..24215
                     /gene="rnb"
                     /locus_tag="OO199_000802"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5534956.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24293..24395)
                     /locus_tag="OO199_000803"
                     /pseudo
     CDS             complement(24293..24395)
                     /locus_tag="OO199_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="OO199_000804"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="OO199_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26571..26744
                     /locus_tag="OO199_000805"
     CDS             26571..26744
                     /locus_tag="OO199_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534957.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="OO199_000806"
     CDS             26834..27583
                     /locus_tag="OO199_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5534958.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="OO199_000807"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="OO199_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5534959.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO199_000808"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO199_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5534960.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO199_000809"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO199_000809"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5534961.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO199_000810"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO199_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5534962.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO199_000811"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO199_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5534963.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO199_000812"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO199_000812"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5534964.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="OO199_000813"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="OO199_000813"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5534965.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO199_000814"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO199_000814"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5534966.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /locus_tag="OO199_000815"
     CDS             complement(35512..35607)
                     /locus_tag="OO199_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534967.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /locus_tag="OO199_000816"
     CDS             complement(35721..35888)
                     /locus_tag="OO199_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5534968.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36055)
                     /locus_tag="OO199_000817"
     CDS             complement(35891..36055)
                     /locus_tag="OO199_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5534969.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO199_000818"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO199_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5534970.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO199_000819"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO199_000819"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5534971.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDTYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="OO199_000820"
     CDS             40511..40762
                     /locus_tag="OO199_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5534972.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO199_000821"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO199_000821"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5534973.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="OO199_000822"
     CDS             42067..42825
                     /locus_tag="OO199_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5534974.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="OO199_000823"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="OO199_000823"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5534975.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO199_000824"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO199_000824"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5534976.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="OO199_000825"
     CDS             complement(44425..45045)
                     /locus_tag="OO199_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5534977.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO199_000826"
     CDS             complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO199_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5534978.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="OO199_000827"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="OO199_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5534979.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="OO199_000828"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="OO199_000828"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5534980.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="OO199_000829"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="OO199_000829"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5534981.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="OO199_000830"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="OO199_000830"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5534982.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="OO199_000831"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="OO199_000831"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5534983.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="OO199_000832"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="OO199_000832"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5534984.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53108..53287
                     /locus_tag="OO199_000833"
     CDS             53108..53287
                     /locus_tag="OO199_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5534985.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53373..53873
                     /locus_tag="OO199_000834"
     CDS             53373..53873
                     /locus_tag="OO199_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5534986.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53919..54425
                     /locus_tag="OO199_000835"
     CDS             53919..54425
                     /locus_tag="OO199_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5534987.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54485..55123)
                     /gene="ompW"
                     /locus_tag="OO199_000836"
     CDS             complement(54485..55123)
                     /gene="ompW"
                     /locus_tag="OO199_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5534988.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55363..55452
                     /locus_tag="OO199_000837"
                     /pseudo
     CDS             55363..55452
                     /locus_tag="OO199_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55480..56223
                     /locus_tag="OO199_000838"
     CDS             55480..56223
                     /locus_tag="OO199_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5534989.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56253..56792
                     /locus_tag="OO199_000839"
     CDS             56253..56792
                     /locus_tag="OO199_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5534990.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56897..57295
                     /gene="yciA"
                     /locus_tag="OO199_000840"
     CDS             56897..57295
                     /gene="yciA"
                     /locus_tag="OO199_000840"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5534991.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57335..>58018)
                     /gene="tonB"
                     /locus_tag="OO199_000841"
     CDS             complement(57335..>58018)
                     /gene="tonB"
                     /locus_tag="OO199_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5534992.1"
                     /translation="TLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLE
                     PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDV
                     KPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRI
                     EGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILF
                     KINGTTEIQ"
BASE COUNT        13906 a        15028 c        14514 g        14570 t
ORIGIN      
        1 ctaatgcacc ttcctgctgt aatgcctcga ccagcgctaa tatttcgctt tctggaagca
       61 agccacgcag tgggttcaac ccgatgccat cgtgagaggc gaggaagtta aaccaggtcg
      121 ttttactgga gggcaagctc agactttgcg cccactggca cagcgcctcg acgttctgtt
      181 tttgcaccgc atgtaacacc agcggcggca gcgagaactg gtacaccata tgcgcttcgt
      241 catcaccgtt gccaaagtaa gcaatgttgt ctttgtgcgg aacattggtt tcggtaatga
      301 tcactgtacc tggtgctacg tcatcaataa tcgaccgtaa cagcttgata atcagatgtg
      361 ttttttccag atggatgcag cttgttcccg gctctttcca cataaagcca acggcatcca
      421 ggcggacata ctcagcacct ttctcaaggt aacagagtaa aacatccacc atcgccagca
      481 acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat
      541 ggcgcgtaga attatcatcc atttgaaacg gcgttaatag cggtaacgca cgcggaaggg
      601 tgacggcact gagatcggtt ttcggatcaa ccgcaataaa gaaatcttca aaacccggaa
      661 gctgttgtaa atagttttta aaccattcac tttttgcaga catatggttg cagacaaaat
      721 caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc cccgcttcac
      781 tggcgacctg atgataatca attaccgaaa atccatcatc agatgaccat gggtaaaaag
      841 gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtagaac tgattaaata
      901 tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtaatt aacacgacat
      961 cactttcatc ccagtgtttt ttacgtctct gtgtaattaa tcgtttcgca ctctcaagac
     1021 gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt tcgtccatgt
     1081 aatccgtaat tttctgtttc atgttgcctt tccgtggtag cggtcccatg aggcgagaga
     1141 ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg caggaaatca
     1201 ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctata gggatgtaaa
     1261 taaggtgtga cagaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct
     1321 ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc
     1381 atctcgctaa ggagctcttt tgcctgcccg gactggcgtc cggcattgca atacactttc
     1441 acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga
     1501 atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc
     1561 cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt
     1621 tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac gcctgagatt
     1681 taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt attagcagcc
     1741 cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg gcgagcaact
     1801 gattttatcg cccagcccgc cacacccgcc gggccatagc gcagtgcggt aagaagtacc
     1861 agcttgcctg ctaatttgaa accaagcttc accttttgcc cggcctgttg ccagcgagta
     1921 ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga
     1981 cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg tcgacttcat
     2041 ccagcaattc gctgctggaa ggtagctgct caccaaaggc catgttgtcc ggcattggat
     2101 caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa
     2161 tcgacagcac caccaggata cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc
     2221 cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataacttt ttattgagat
     2281 taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa tagattccag
     2341 cgcctgaata cgttcgcgca tccgttttgc ttcatcaacc agttgcgcta atcgctgctg
     2401 ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc ataaccagat
     2461 cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg cgctcataag
     2521 tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctggatgtta ttattgattg
     2581 tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc atcggctttc
     2641 agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct ttctgcttcc
     2701 gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc ttcatccagt
     2761 ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac
     2821 atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc gccaatctct
     2881 ttcttcatgc gtgccagcgt atcgtccacc agcgtcactt catgttccag ggacttaatc
     2941 agatcggtca gtttctgttt ttcaattaac gctgcacgtg ccagatcctc tctctctttc
     3001 agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc ttgttcaata
     3061 cggcgagtca gctgtttctt ttctgccaac gcacgcgccg aagtagaacg tacttcaacc
     3121 agtgtatctt ccatctcctg gatcatcaga cgcaccagtt tctgtggatc ttccgctttc
     3181 tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa aatacccata
     3241 atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa tacaatttgc
     3301 gtgccaattt tttatctttt tgatttataa agatctgatt gaagaatcaa cagcaacatg
     3361 ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt tcatcatggc cgaatacaaa
     3421 gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc
     3481 gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt
     3541 gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc ccttaactgc
     3601 gcggcgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc gggggcgttt
     3661 accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg ccgacggcgg tacgctattt
     3721 cttgatgaac tcgctacggc accgatgatg gtgcaggaaa aattattgcg tgtgattgag
     3781 tacggtgaac tggagcgcgt tggcggtagc cagccgttgc aggtgaatgt gcggttggta
     3841 tgcgcgacga atgccgatct cccggcgatg gtcaatgaag gtacttttcg cgctgacctg
     3901 ctcgaccggc tggctttcga tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac
     3961 attatgctga tggcagaaca ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg
     4021 ttcccggggt ttacggagcg cgccagagaa acattgctga attatcgttg gccgggaaat
     4081 attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac cagcgattat
     4141 ccgcttgatg acatcattat tgatcccttt aaacggcgtc cacctgaaga agccatcgcc
     4201 gtttcagaaa atacctcgct tccgacgctg ccgctggatt tacgcgagtt tcagatgcag
     4261 caggaaaaag agttgctgca actcagtttg caacagggga aatataacca gaagcgcgcg
     4321 gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt
     4381 tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt cggcatacat
     4441 aatcaatacg tcgtttgcag gcctgataag cgcagcgcat cagacattat tgcgttgggc
     4501 tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggg
     4561 atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc
     4621 agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
     4681 gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg
     4741 gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc cagttgattc
     4801 gcgcgttcgc agagtgattc tttgtcgata atattgagca ccgcgtgcgc ggcagccacc
     4861 gccagcgggt tcccggcgta ggtgccaccc aacccgcccg gcgcgggcgc gtccataata
     4921 ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc
     4981 attaagtccg gcttatcggc gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag
     5041 ccgctttgca cttcatcggc aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg
     5101 gcggcaacca gctcttttgg cgcaacgttg aaaccgcact cgccctgcac cggttcgaaa
     5161 ataatggccg cgacctgctt cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg
     5221 agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa
     5281 ccagggaacg ggccgaagcc gattttgtac ggcgcaactt ttccggtcag cgccatggtc
     5341 atatatgtac gaccgtggaa gccaccgcta aacgcaatca cgccaggtcg tccggtatgg
     5401 gcgcgagcaa ttttcaccgc gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt
     5461 ttagcctgcc cgctcactgg ggcaagggtg ttaattttct ccgccagcgt gacgtagctt
     5521 tcgtacggca caatctgata cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc
     5581 gcgaccagat cagggtggcg atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg
     5641 atgtattcgt tgccctctac gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag
     5701 ttacacatca cgccaacccc gcgtggggta gcagaaagac gacgctgatg gaattcattg
     5761 ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa cgcccagacg
     5821 atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc
     5881 gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg
     5941 cagcagttca gcaatcaaac gcccggcaag atgggtacag gtgacgccgt ggccgctgta
     6001 accctgcatg taatagatgt tggtatcaag gcggccgaac tgcggcatgt gcgacaaggt
     6061 cagcaggaag ttgcccgtcc agcggtaatc aattttcacg cccttcaact gagggaaggt
     6121 ttttagtagt ttcggtacca caagacgctc aacgtcatcc gggttgcgtg caccatacac
     6181 tacacccccg ccgtacagca ggcggttgtc ggcggtaaga cggtagtaat caagcagata
     6241 gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat cttccgacag
     6301 ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcta gttccggctc
     6361 tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg tcacctgacc
     6421 attggcagtc cgaaccacgg ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc
     6481 atacacgcgc ccgccgttca ggcggatggc atcggcttca ccaatggcga ggtttagcgg
     6541 gtgaatatgc ccaccgctgt gatccagcag cgcaccagtg tagcgatcgc tggcgacctc
     6601 gcggcgaata gcgttgacgt ccagcagctc cagctgttta ttgccgtagc gttcccagtt
     6661 ctctttctgc tcttcgagtg tggcgagttg tttatcattc atcgccacaa acagaccgcc
     6721 ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga tctcgccacc
     6781 ctcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac
     6841 gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa
     6901 gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca gatgcagcgc
     6961 ggaggagagc ccggtatagc cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt
     7021 cagtgtgtcg aatggtgcat atttattcgc actggcggcg tagtaactgc tggtatgttc
     7081 ggtcatttca ggcctccagg cttatccaga tggttttcag ttcagtgaat ttttcaaggg
     7141 catgcaggga tttgtcgcga ccgttgccgc tctgcttata gccgccaaac ggcacggtca
     7201 tatcgccgtc gttgtagtta ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc
     7261 ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt
     7321 tggcaagctg cagcgcctga tcttctgatg tgaaacgggt gaccaccagc accggaccga
     7381 aaatctcttc gcggcttaag gacgcattcg ggtccacatc cacgaagatg gtcgggccga
     7441 tggccgctgc cagcccggcg ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt
     7501 cacgaataaa ggtgtgtacc gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg
     7561 ttgcgggatc aagtggatgg cccggctgcc agttttgcgc ctgctgtttt aacagggcta
     7621 agaattcatc ggcgatgctc tcttccagta acaaacgcgt tccggcgatg cacacctggc
     7681 cctggttgta gaagatgcct gcggcggtgg cgcttgccgc tttttgcaga tccggacaat
     7741 cggcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacacgt ttcatgttgc
     7801 tatcacccgc gtctttcagc agttgtttac ccgtacgcgt ggagccggta aaggcgatgg
     7861 catcgatatc gttatgacgc gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca
     7921 cgttcaacac accatccggc aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac
     7981 tgagcggtga tttttcagac ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc
     8041 cgagtttcca gcaagtcagc aacagcggga agttccacgg cacgatggcg gcaatcacgc
     8101 cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc acttcgccat
     8161 acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat
     8221 cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt gccagctctt
     8281 cggcgtgggc ttccattaaa tcggcgagtt tattcagtac cgctttacgt ttagccggag
     8341 aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga
     8401 tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg
     8461 tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat aagcggtttt
     8521 caatggcgag acttaacgct ttatcctgcc agtaagccag atgatgaaaa ttcattatga
     8581 ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc agagatcccc
     8641 gggctgcatg cccgtttatt tctggcgaca ttctttatgg attgccgggc gcattgcgct
     8701 tacccggctt acagaacaaa aagattaaaa cgtggtgggc gtatgggcgc tgataattcg
     8761 gcaaatacct gccgacgtat tactgaaact gtgcgggatg ccggtattaa tggcataact
     8821 ttgccccgcg acgaggtggt aatcctgacc attaatcgtc agaacaattt caccttccag
     8881 tactgtgcct atttcctcac cctgatgctt aattctttcc ccagtggttg tgcccggctg
     8941 gtacgtttca aagatcatcg ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat
     9001 tgacacaccc tgactgccca tctcaattaa gtcgtcctga ttaatgacga cctgcggctc
     9061 atcaggtttt tccggctcgg aaaagaattc cgagagtgac aaaccataca ccttcagcag
     9121 cttttgcagc gtactgatgg cagggctgac tttatcttgt tctatcgtac tgatagcact
     9181 gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa agcccctgct gctggcggat
     9241 ttccgacaag cgttttcctg gcgccagtcc ctcatcactc atatttgcat ttccttaaac
     9301 tgtagtggtc agagtcgctg tttttcagcg atatggtgct gccaagcggt gataaagccc
     9361 tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc attccgggtg ccactgtacg
     9421 cccagcgcaa aaggatgatt gatgacgcta accgcctcaa ctaagccatc cggcgagcga
     9481 gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt gtccatgtag agagtttacc
     9541 caaaagttgc tacattcagg taacaacgca gacagtaatc ccccctcttc aacctgaact
     9601 tcatgcgacg gtgcatattg ctgttccacc ggcagttcgg gatcttcccg atgttccagc
     9661 aattcaggct gttcgcacag cttgcgatgc aacgaaccac cggttgccac caccagttct
     9721 tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt cgagtgcggc attaattatc
     9781 gccatgctca gaagatcacg cccgggatcg gcgtcaggct catcgccgtt ttcaccatat
     9841 aggtgcggct gcacattgct gggactacta ggaagataaa tgccatcgag tttcggcaaa
     9901 agttgttcaa gtaacgacgg ttccgccagc gcatgtggta gcgcaattgg caagccgcct
     9961 gcgtggatga tggcattcag gtacttttct tgcagagtct gggtcgcatg acccttaagc
    10021 ctgttcctgc acattacgac accgataacc ggattgttca ttatattttc catgctcaat
    10081 ctcacaaagt ggactaaatt atcgccatta ctgcgcttta aatgaccttt atgttcaata
    10141 ttttttcaat ctagcagtgg attttcattt ttgcaaactc aatttaacat ttgacaaaca
    10201 tttggtttgc atacagattc gaatgatggt cattatattt tacgctttga taacgagcgg
    10261 aaaacaaacc aaaggcgaag aatcatggaa accaatatcg ttgaagtaga gaactttgtt
    10321 cagcagtcag aagagaggcg gggtagcacc tttacgcagg aagtgaaacg ctacctggag
    10381 cgctacccga atacgcaata tgttgatgtt ttacttactg atttgaatgg ttgcttccgt
    10441 ggaaaacgta tccctgtttc aagcctgaag aagctcgaga aagggtgtta tttcccggcc
    10501 tcggtatttg caatggatat tctgggcaac gtagtagaag aagcaggtct gggtcaggaa
    10561 atgggcgagc cggatcgtac ctgtgttcct gttctcggtt ccttaactcc ttctgccgcc
    10621 gatccagagt ttatcggtca gatgctcctg accatggtcg atgaagatgg cgctcccttt
    10681 gacgttgagc cgcggaacgt tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg
    10741 ttcccggttg tagcggtaga gctggagttc tatttactgg atcgccagcg cgacgctgaa
    10801 ggggatctgc aaccgccctg cgcgcctggc accgatgacc gcaatacaca aagccaggtt
    10861 tactccgttg ataacctcaa ccacttcgcc gacgtgctca atgatattga tgaactggcg
    10921 cagttgcagc tgattccggc agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa
    10981 atcaaccttt accatactga taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa
    11041 cgcctagtgc gtctgatggc agaaaagcat aagatgcacg ccacttttat ggcgaagccg
    11101 tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa taatcgtggc
    11161 gagaatgtgc tttctgacac agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc
    11221 gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat
    11281 cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc
    11341 accgtcgccc tgcgtattcc gtgcggtgac cgtcataatc accgcgtgga atatcacgtg
    11401 gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg tattttgcat
    11461 ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa
    11521 ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga gaatgatcac
    11581 ctgcgccgct atttaggcga acggttttgc catgtgtatc acgcctgtaa aaatgatgag
    11641 ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt gaaaaacgcg
    11701 taattcccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga catttgctgg
    11761 taatccagac atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta
    11821 acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc cgcatggcga
    11881 gggaggctgc gtatgcgccc ctcctgctac tgactggact ccgccccctt tgcttccctt
    11941 gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca ggggaggcag
    12001 caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa acccgtctgc
    12061 gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc acgccgatga
    12121 ccgtatttga tacttttggt attgtctccg gcattagcga cggtcacgtt ccggcgtcct
    12181 atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc
    12241 agtttccgga ggccggttcg gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg
    12301 gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg atcaacgttt
    12361 tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg
    12421 taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg gtcgctaact
    12481 tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg
    12541 ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca
    12601 gcgagaacgc gcacctgatc ccgattatta ccggtgcgac gattgtctgt ttctcgttcc
    12661 tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc
    12721 cgaaagccat cttcctgacg gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct
    12781 ttatgcagct gttctttccc gatatcagcc gctttaaaga cccggacgcc gcactgcctg
    12841 aaattgcgct gtacgtcggc ggcaagctct ttcagtcgat tttcctctgc accacgtttg
    12901 tgaacacatt agcgtctggc ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga
    12961 tggggcgtga caatgtgttt ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga
    13021 ctccggcact caacgtcatt atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt
    13081 tagtcaccgc gacagcactg attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc
    13141 tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa taaaagctgg aaggatcact
    13201 tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg tgggttaacc
    13261 tagagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc gcatatttgt
    13321 ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga acgccggtga
    13381 gcgaaacgta atagccggat gcgcggcgca ttatcctgcc tgagggtaac gcgccgcgcc
    13441 attaactata aaaagatgta tgacccggat cacgagtgct atcatccgcc cttatgctta
    13501 gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata ttttgatatt
    13561 gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg cacaccgctg
    13621 gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat cagcgaagaa
    13681 gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga tgagatcgca
    13741 gagtttttga atcaatgggg taatgagtaa ttatttaatt gcagacgatc ggtcagtatc
    13801 gttgtccgga taaagcgttc acgccgcatc cggctaacaa tgacacgcac tgcagcgtgc
    13861 tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc taagttgttc tgtgagttat
    13921 agttatagag aaatcacggg tacgaatgtt gaacagatgg cgacgccacc tgttcaacga
    13981 ccaaccacac ggccctcatt acgacccctg aacacaaacg gtaatggcaa atgtcattaa
    14041 atattagtaa gatagcctct ttacataaaa tcccttaata ttatgcgcca ggtattatcg
    14101 tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa tcgccgcgcc tgaatccccc
    14161 ccgcatgctg atatccgcga cagcggtttt gtctattgcg tcagcgggca agtcaacacc
    14221 tttaacccat ccaaagcgag cagtgggtta attgtcgata cccttgccgc ccagttttat
    14281 gatcgactgc tggatgtcga tccctatacc tatcgcctga tgcctgaact tgccgaaagc
    14341 tgggaagtac tcgacaacgg cgcgacctat cgcttccacc tgcgtcgcga tgttccgttt
    14401 caaaaaaccg actggtttac tcccactcgt aaaatgaatg ccgacgatgt ggtgtttacc
    14461 ttccagcgaa tttttgaccg caacaacccg tggcataacg tcaacggcag caacttcccc
    14521 tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg tccgcaaact ggataatcat
    14581 accgttgagt tccgactcgc ccagccggat gcttcttttt tgtggcacct cgcaacccat
    14641 tatgcttcgg tcatgtcggc agaatatgcc cggaagttag agaaagaaga tcgccaggag
    14701 caactcgacc gtcaaccggt cggcaccggt ccgtatcagt tgtcggaata ccgcgccggg
    14761 caatttattc gcctacaacg tcatgatgac ttctggcgcg gtaaaccgtt aatgccgcag
    14821 gtagtggtgg atttaggctc cggcggcacc ggacgtctgt cgaaactcct gaccggggaa
    14881 tgcgacgttc tggcctggcc tgctgccagc cagctatcca ttttgcgtga cgacccgcgc
    14941 ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc tggcatttaa caccgccaaa
    15001 ccgccgctaa ataatcccgc tgtccgccat gcactggcac tggcgattaa taaccagcgc
    15061 ctgatgcaat ccatctatta tggtacggct gaaacggcgg cctctatttt accgcgcgcc
    15121 tcgtgggcct atgacaacga ggctaaaatt actgaataca atccggcgaa atcgcgcgaa
    15181 cagttgaagg cgttggggct ggaaaactta acgctgaaac tgtgggtgcc cacacgttcg
    15241 caggcgtgga accccagtcc actgaaaact gccgaactga ttcaggcgga tatggcgcag
    15301 gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct ttcaggaggc gcggttgatg
    15361 gatatgagcc atgatctgac gttatccggt tgggcgacgg acagtaacga cccggacagt
    15421 ttcttccgtc cgttactgag ctgcgcggca attcattcac agaccaacct cgcccactgg
    15481 tgcgatccga aattcgacag cgtgttgcgt aaggcgctct cctcgcagca gctggcagcg
    15541 cgtattgaag cctatgacga agcgcagagt attctggcgc aggaattacc tattctgccg
    15601 ctggcgtcgt cattgcgttt gcaggcctat cggtacgata tcaaaggtct ggtacttagc
    15661 ccgtttggta acgcctcctt tgctggggtg tatcgcgaga aacaggatga ggtgaaaaaa
    15721 ccatgattat cttcacctta cgccgcattt tgttattgat tgtcaccttg ttcctgctga
    15781 ccttcgttgg cttcagcttg agctatttca ctccacacgc gccgttacaa ggcgcgtcac
    15841 tgtggaatgc ctgggtgttc tggtttaatg gcctgatcca ctgggatttt ggtgtttcca
    15901 gcattaacgg tcaaccaatt gccgaacagt taaaagagct cttcccggcg acgatagaac
    15961 tgtgcatcct cgccttcggt tttgcgctga ttgttggcat ccccgtgggg atgattgccg
    16021 ggattacgcg ccataagtgg caggataact tgatcaacgc catcgcctta ctgggctttt
    16081 caatcccggt gttctggctg gcgcttctgt tgacactgtt ttgttcactc acgctaggtt
    16141 ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt gaaaccgatt accggttttg
    16201 cgttgattga tgcctggctt tcggactcac catggcggga tgaaatgatc atgagcgcaa
    16261 tccgccatat gatattgccc gtgatcaccc tgtcggttgc gccaacaact gaagtgatcc
    16321 gcctgatgcg tatcagcact attgaagtgt atgaccagaa ctatgtaaaa gcggcggcga
    16381 cccgcggttt gtcgcgcttt actattttgc gtcgccacgt tctgcataac gcgttaccgc
    16441 cggttattcc tcgcctgggc ttacagtttt ccaccatgct gacgctggcg atgatcaccg
    16501 aaatggtttt tagctggccg ggtttagggc gctggttaat taacgccatc cgccagcagg
    16561 attacgcagc catttccgcc ggagtgatgg tgtgtggctc actggtcatt attgttaacg
    16621 tgatttctga tattttgggt gccatggcta accctctgaa acataaggaa tggtatgcct
    16681 tacgatagcg tatacagcga aaagcgcccg ccgggcacgc tgcgtaccgc ctggcgcaaa
    16741 ttttatagtg acgcctctgc gatggtcggc ctgtacggct gcgcgggact ggctgtactg
    16801 tgtatttttg gcggctggtt tgcgccttac ggcatcgacc agcaatttct cggttatcaa
    16861 ttattgccgc catcatggtc gcgctatggc gaagtttctt tcttcctggg gactgacgac
    16921 ctcggacgtg atgttttaag ccgtttactg agcggagctg cgccaaccgt gggtggcgca
    16981 tttgtggtga cgcttgccgc gacgatctgt ggcctggtac tgggcacctt cgccggggcg
    17041 acacacggcc tccgctcagc ggtgcttaac catattctgg ataccttact ggcgatccct
    17101 tcgctgctgt tggcaattat cgttgtagcg tttgccggac cgagtttgtc ccacgctatg
    17161 tttgctgtct ggctggcatt gctgccgcgt atggtacgtt cgatttacag catggtgcat
    17221 gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg atggcgcatc aacgctgaat
    17281 attctctggt ttgctgtgat gccaaacatc accgccgggc tggtcaccga gatcacccgc
    17341 gcactgtcga tggcaattct cgatatcgcc gcgctgggct ttctcgatct cggcgcacaa
    17401 ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc tggaactgat ttatgtcgcc
    17461 ccgtggactg tcatgctgcc aggtgcggca attatgatta gcgtgttgtt ggttaacctc
    17521 cttggcgatg gcgttcgtcg tgcgattatt gcgggggtgg aataatgcca ttactcgata
    17581 ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg ggttaaagcc gtcgaccggg
    17641 taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt tggtgaatcc ggttccggca
    17701 agagtttgat tgcgaaagcg atttgtgggg tgaataaaga taactggcgt gttactgctg
    17761 accgtatgcg ttttgatgat atcgatttgc tgcgtctctc cgcacgcgaa cggcgcaaac
    17821 tggttggcca taacgtgtcg atgattttcc aggaaccgca gtcgtgtctt gacccttcag
    17881 aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg gacctataaa ggccgttggt
    17941 ggcagcgttt tggctggcgc aaacgccgtg ccattgaact gctgcaccgc gtggggatta
    18001 aagatcacaa agatgcgatg cgcagttttc cctatgagtt gaccgaaggt gaatgtcaga
    18061 aagtgatgat agccattgca ctggcgaatc aaccgcgtct gctgattgct gacgaaccga
    18121 caaactcaat ggagccaaca acccaggcgc aaatctttcg cctgctgacg cgtctcaacc
    18181 aaaacagcaa taccactatt ttgcttatca gccatgactt acaaatgctt agccaatggg
    18241 cggataaaat taacgtgctt tactgcggtc aaacagtgga aaccgcgccg agtaaggagt
    18301 tggtgacgat gccacatcat ccttataccc aggcgctgat ccgcgcgata ccagacttcg
    18361 gcagcgcgat gccgcataaa agtcgcctca atactctacc cggcgctatc ccattgctgg
    18421 aacagttacc gattggttgt cgtctggggc cacgttgccc gtatgcacaa cgagaatgca
    18481 ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta tgcctgtcat ttcccgctga
    18541 acatggagaa aaagtgagat gatcgaaacg ctgcttgaag tgcgtaattt aagtaaaacg
    18601 ttccgctacc ggaccggttg gtttcgtcgt cagaccgtag aagcggtaaa acccttgagc
    18661 tttacgctac gtgaaggcca gacactggcg attattggcg agaatggttc gggtaaatcc
    18721 acactggcaa aaatgctggc gggaatgata gaacctacca gcggcgagtt attgattgac
    18781 gatcatccgc tgcattttgg cgattattcc ttccgtagtc agcgtattcg catgattttt
    18841 caggatcctt cgacctcgtt gaatccccgt caacgtatct cacaaattct ggattttcca
    18901 ctgcgcctga acaccgacct ggaaccggaa cagcgtcgca aacagattat tgaaacgatg
    18961 cgtatggttg ggctgctacc ggatcacgtc agttactatc cgcatatgtt ggcacccgga
    19021 caaaaacagc gtctgggtct ggcgcgcgcg ctgatattgc gcccaaaagt cattattgcc
    19081 gatgaagccc tcgcttcact ggatatgtca atgcgttcgc agttgattaa tctgatgctg
    19141 gagttacagg aaaaacaggg catttcgtat atttatgtta cccagcatat cggaatgatg
    19201 aagcacatta gcgaccaggt gctggtgatg catcagggcg aggttgtcga gcgaggcagc
    19261 accgcggatg tgctggcatc gccgctgcat gaactcacca aacggctgat tgccgggcac
    19321 tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc gctaattttc tgaatcctcc
    19381 ttccagatta ttcacctcat tctgacaaga gattaaatct tgtcagaatg aggtgatgga
    19441 taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc actttgcagg aacgaaagct
    19501 ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa tttcgccgtc aacatccggt
    19561 ggggagctac attctcgatt ttgcttgctg ctcggcgcgc gtagtcgttg agctggatgg
    19621 tgggcagcat gatttggcag ttgcctatga taccaggcgc actagctggc ttgagtcgca
    19681 gggctggacc gtgctgcgtt tctggaataa cgagattgat tgtaatgagg aggcggtgct
    19741 ggagattatt ctgcaggaac tgaaccgccg gtcaccctca ccctgaaagg gcgagggggc
    19801 cgaccgagcc gaatagctgt tgtggtgaaa acatggagac agtgctggag aatattcggc
    19861 aaggtctgaa ccatcccagc catcgccata aaagggttag gggctgtatg agcctgtttg
    19921 ttgctggggt aacaatattt gcacaatatg gtcccctcgc ccctctgggg agagggttag
    19981 ggtaagggga aaagcgcccc ccctgccgca gcctgctccg gtcggacctg gcaactatag
    20041 ctactcacag ccaggttgat tataataacc gtttatctgt tcgtactgtt tactaaaacg
    20101 acgaatcgcc tgattttcag gcacaacaag catcaacaat aaggattaaa gctatgggtt
    20161 ttctttccgg taagcgcatt ctggtaaccg gtgttgccag caaactgtcc atcgcctacg
    20221 gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc attcacctac cagaacgaca
    20281 aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc gttctgcagt
    20341 gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc tgaactgggg aaagtttggc
    20401 cgaaatttga cggtttcgta cactctattg gttttgcacc tggcgatcag ctggatggtg
    20461 actatgttaa cgccgttacc cgtgaaggct tcaaaattgc ccacgacatc agctcctaca
    20521 gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa tccgggttct gccctgctga
    20581 ccctttccta ccttggcgct gagcgcgcta tcccgaacta caacgttatg ggtctggcaa
    20641 aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg gaaggtgtgc
    20701 gtgttaacgc catctctgct ggtccgatcc gtactctggc ggcctccggt atcaaagact
    20761 tccgcaaaat gctggctcat tgcgaagccg ttaccccgat tcgccgtaca gttactattg
    20821 aagatgtggg taactctgcg gcattcctgt gctccgatct ctctgccggt atctccggtg
    20881 aagtagtcca cgttgacggc ggtttcagca tcgctgcaat gaacgaactc gaactgaaat
    20941 aatcgttctg ttggtaaaga tgggcggcgt tctgccgccc gttatctctg ttataccttt
    21001 ctgatatttg ttatcgccga tccgtctttc tccccttccc gccttgcgtc aggataacga
    21061 tttcctttac gaccaaggag cgcccatgga acaacgccac atcaccggca aaagccactg
    21121 gtatcatgaa acgcaatcca gtactacgga gtatgacgtt ctgcctctgg tcccggaagc
    21181 cgcaaaggtc agcgatccct ttctgctcga cgtgatcctt gatgaagaga cgctggcccc
    21241 cttcctttca tggctggtcc ctgcgcgcgt tcttgcagtg gagttgttcc ctgaccagct
    21301 taccgtgacc cgttcacaaa ccttcaccgc ttatgaacgc ttgtcgacgg ccctgacggt
    21361 tgctcaggtt tgcggcgtcc agcggttatg taactactat tcggcgcgac ttacgccgct
    21421 ccccggacct gattcctcca gggaaagtaa tcatcggttg gcacaaatca cgcaatatgc
    21481 ccgccaactg gctagctcgc cttctattat cgacaaccga tcgcgccagc atctgaatga
    21541 cgtcggtctt actgtctggg actgtgtgat cattaaccaa atcattggtt ttattggctt
    21601 tcaggcgcgg acaattgcga catttcaggc ttatctcggg catccggtac gctggttacc
    21661 cgggctggcg atacaaaact acgccgacgc gtcactgttt gctgatgaat caatacgctg
    21721 gcgaagcagc tatgaggtgg aaaaactacc tgaagagcac acaaaaagtt caactgcaga
    21781 actttgccaa ctggccgaaa cacactctct ccaccctatt tcactttccc ttctcgaaaa
    21841 gttgttaaac agcacacggg tcaatgcaca gccggataat cagcttgcgg cgttgttatg
    21901 cgcgcgtata aatggcagtc ctgcttgttt ttccacctgt atggattcat caaatgaata
    21961 taaaaaaatc agcacccttc tgcgcaaggg cgaaaatgaa attaaccgat gggctgaccg
    22021 tcattctgtt gagcgcgcta ccgttcaggc gatacaatgg ctgacccgag cacccgatcg
    22081 ctttagcgcc gcccagttca gccctttact cgaacacgaa aaatcatcaa cgcagattat
    22141 taatctgctg gtatggagcg ggctgtgtgg ctggataaat cgcttaaaaa tcgcgttggg
    22201 tgagacatat taaccttgcc gcgtcagaca gattcgcgta aaactgtcag ccgctctaat
    22261 ggccaccaaa atagacaatt atgtttcagg acaacccgct gctagcgcag cttaaacagc
    22321 aactgcattc ccagacgcca cgcgctgaag gggtggtaaa agccacagaa aaaggctttg
    22381 gcttcctgga agtcgacgca caaaaaagtt atttcattcc gccgccgcag atgaaaaaag
    22441 tcatgcatgg cgaccgaatt atcgcggtga tccacagtga aaaagaacgt gaatccgcag
    22501 agccagaaga actggttgaa ccgttcctga ctcgtttcgt gggtaaagtt cagggcaaaa
    22561 atgaccgtct gaccatcgtt cctgatcatc cacttttaaa agacgccatt ccttgccgcg
    22621 cagcccgtgg cctgaaccac gagtttaaag aaggcgactg ggcggttgcc gaaatgcgcc
    22681 gtcatccgct gaaaggcgat cgttctttct atgctgaact gacacaatac atcacttttg
    22741 gtgacgacca ctttgtaccg tggtgggtta cccttgcgcg ccataatctg gaaaaagaag
    22801 caccagacgg cgtcgctacc gaaatgctcg atgaaggtct ggttcgtgaa gatctgacct
    22861 cgctggattt tgtcaccatc gacagtgcca gcactgaaga tatggatgac gcccttttcg
    22921 ctaaggcgct gccggatgac aaacttcagc tgattgtggc gattgccgat ccaaccgcgt
    22981 ggattgctga aggcagcaag ctggacaaag ccgcgaaaat tcgcgcattc accaactatc
    23041 tgcctggctt caacatccct atgctgcctc gcgagctttc tgacgatctc tgctcactgc
    23101 gcgccaatga agtccgcccg gtactggcat gccgcatgac gctctccgct gatggcacca
    23161 ctgaagataa tatcgaattc tttgccgcca ccatcgaatc caaagcgaag ctggtgtatg
    23221 accaggtttc tgactggctg gaaaataccg gtgactggaa gcctgaaagt gaagcaattg
    23281 ccgaacaagt ccgtttgcta gcgcaaattt gccaacgccg cggcgagtgg cgtcataacc
    23341 acgcactggt gtttaaagat cgcccggatt accgctttat tctcggtgaa aaaggtgaag
    23401 tgctggatat cgtcgccgag cctcgtcgca ttgccaaccg tatcgtcgaa gaagcgatga
    23461 ttgccgctaa catttgtgcg gcccgcgtac tgcgcgataa gctcggtttt ggcatctata
    23521 acgtgcatat gggctttgat ccggcgaatg ccgacgcgct ggcagcgttg ctgaaaacgc
    23581 acggtctgca tgtcgatgcc gaagaagtgc tcacgctgga cggtttctgc aaactgcgtc
    23641 gtgaactgga tgcgcaacca actggtttcc tcgacagccg cattcgtcgc ttccagtcat
    23701 ttgctgaaat tagctctgaa cccggtcctc actttggcct cggtctggaa gcatacgcca
    23761 cctggacttc gccgatccgt aaatatggcg acatgatcaa ccaccgtctg ctgaaagcgg
    23821 ttatcaaagg cgaaactgcg acgcgtccgc aggatgaaat cactgtccaa atggccgaac
    23881 gtcgccgtct caaccggatg gcagaacgtg atgttggcga ctggttatac gcacgcttcc
    23941 tgaaagataa agccgggacc ggcacccgtt tcgcggcgga aattgtcgat atcagccgtg
    24001 gcggcatgcg tgtgcgtttg gttgataacg gcgctatcgc ctttattccg gcacctttct
    24061 tacacgctgt gcgcgatgaa ctggtttgca gccaggaaaa cggcactgta caaattaaag
    24121 gtgaaacggt ttacaaagta actgacgtta ttgacgtcac cattgccgaa gtccgcatgg
    24181 aaacccgcag cattattgcg cgcccggtcg cgtaatctcc tttcacggcc cattcctcat
    24241 ggatgggccg tttatttccc cgctctacct tcgttatatc caggcagatt ttttaactga
    24301 ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat ataaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtggcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttagactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcagtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgtcgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt ccagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagt atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcacaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaacgcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt gcgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gtaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttt ccctctatct tctcgttgcg cttaatttga ctaattctca ttagcgacta
    53041 attttaatga gtgtcgacac acaacactca tattaatgaa acaatgcaac gcaacgggag
    53101 aaataacatg gccgaacatc gtggtagttc aggaaatttc gccgaagacc gtgagaaggc
    53161 atccgacgca ggccgtaaag gcggtcagca tagcggcggt aattttaaaa atgatccgca
    53221 acgcgcatct gaagcgggta aaaaaggcgg tcaacaaagc ggtggtaata aatcaggcaa
    53281 atcctgattc accgttaatt attaatatca gctgataaag ataattgcat ctgcgggccg
    53341 tatggctcgc agtgatttca ctggagaaaa atatgaatat gaagaccatt gaagatgtat
    53401 ttattcaact gctttcagat acctacagcg cagaaaaaca attaacccgg gcactggcaa
    53461 aactcgcaag agcaacatca aatgaaaaat taagtcaggc ttttcatgcg cacctcgagg
    53521 aaactcatgg acagattgaa cgaattgatc aagttgtgga atcagaatca aatctgaaaa
    53581 ttaagcgcat gaaatgtgtg gcaatggaag gtcttattga agaagctaat gaggtcatcg
    53641 aaagtaccga gaaaaacgaa gtgcgtgatg ccgcactgat tgccgcagca cagaaagtcg
    53701 agcattatga gattgccagt tacgggacat tagcgacgct ggctgaacaa ttaggttacc
    53761 gtaaagcagc gaagcttctg aaagaaaccc tggaagaaga aaaggccacc gacatcaaac
    53821 tgactgatct ggcccttaat aacgtaaata agaaagccga aaataaagcc tgaaatatga
    53881 attttaactt ttagtcattt tataaagagg acattttcat gaatcgtatt gaacattatc
    53941 atgactggtt acgtgacgcc cacgcaatgg aaaagcaagc cgaatctatg cttgaatcca
    54001 tggccagccg tatagataat tatcctgaac tacgcgctcg tattgaacaa catcttagtg
    54061 aaaccaaaaa ccagattgtt caactggaaa ctattcttga tcgtaatgac atttcacgtt
    54121 cagtcattaa agattccatg agtaaaatgg ctgcgcttgg gcagtcaatc ggtggtatat
    54181 tcccttctga tgaaatagtt aaaggctcta ttggcggata tgtcttcgag caatttgaaa
    54241 tcacctgtta cacctcacta ttagcagcag caaaaaatgc cggtgataca gtctcaattc
    54301 cgatcatcga agcgatttta aatgaggaaa agcaaatggc cgactggctg attcagcata
    54361 ttccgcaaac aactgagaaa tttttaattc gttctgaaac tgatggcgta gaagcgaaga
    54421 aataataccc tttataccat gtccttattg accccgtata ttacggggtc gtttttgtgc
    54481 ggaattaaaa acgatatcct gctgagaaca taaacaccca cggatcgagg cgtacgctat
    54541 cgtgttgctg tgcaccgcct agcttataat tggcggtggt atcgatatcc atgtaccaca
    54601 ctgacatgtt aaccaaccag tcacggttaa tcagataatc aacccccacc tgcccggcag
    54661 ctccccagga atctttcaga ctgagatcgg aaagccccgc ctctttgcca tgatcgttaa
    54721 atccattatc aaagaaggtg gtgtagttaa taccggcccc aacgtaagga cggaatttgc
    54781 tgctggcatc accaaaatac cactgcgcca tcagtgttgg cggcagatgg tgaacggttg
    54841 caatatcgcc ggtcgcccgg gtgccgattt tatggcggaa cggcgtcgct gccagtaatt
    54901 ccacaccaat gttgtcggtc gccatataag taaacgtcaa gcccagttga gtgttattgg
    54961 tcacgctgaa tccacccaga cttcctaacg taccaccagc accttctgtt ggacgtacgg
    55021 ttgcagaacc tgcacgcata aaaaattcgc ctgcttcatg cgcaaaggca ctgccagaaa
    55081 gaagagttgt tactgccaaa gccgccactg ttaacttttt catatccgct ccgtcgttat
    55141 ggttataaaa acgtgacgaa tatacctaca ttggagtaac aaatgatcct acatagatca
    55201 cattacatat gataattcac catttattga tctggattaa tttagaagtt gcattgagaa
    55261 tcctggttaa tgtgatttaa atcaattttt aaatattttc tctacgcaaa tattaactcg
    55321 atcgccactt gccgcggttt gcccacgcct gaaggccagc aattacctcc gctttacaaa
    55381 gatatgcttt tccatacaag acctgatgct gccacccgag gcttaaccag ggtacaattg
    55441 cccgctaatt aacacctgca taaaactcaa ggagagtgca tgtctatcac ggcgcagtcc
    55501 gtataccgtg acaccggaaa tttcttccgt aatcaattta tgaccattct gttggtatcg
    55561 ttgctatgtg cgtttatcac agtggtgtta gggcatgttt tctcacccag tgatgcacag
    55621 cttgcgcagc tcaatgacgg cgtgcccgtt agcggcagta gtgggttgtt cgacctggtt
    55681 cagaatatgt caccggaaca gcaacaaatt ttgctgcagg cttcagcggc gtccactttt
    55741 tcagaattaa tcggtaacgc cattctcgcc ggaggcgtaa tattaattat ccagctggtg
    55801 tctgcgggtc agagagtcag tgcgctgcga gctattggtg ccagtgcgcc gatattgccc
    55861 aagttattta ttctgatttt tctgactacc cttttagtac agattggcat catgctggtg
    55921 gtcgttccgg gaattatcat ggccatttta ctggccctgg caccggtgat gctggtacag
    55981 gacaaaatgg gcatttttgc ctcgatgcgt agcagtatgc ggctgacttg ggcgaatatg
    56041 cgtctggtgg cacccgcagt actgagctgg ttgctggcaa aaacactgtt gctgcttttt
    56101 gcctcttctt ttgccgcatt aaccccggaa attggtgccg tactggcgaa caccttgagc
    56161 aacctgattt ctgccatatt gctcatctat ctgttccgcc tgtatatgtt gattcgccaa
    56221 taaccttaag catttgatta cggaatcgta aaatgaagca gtttcttgat tttttaccgc
    56281 tggttgtctt tttcgcgttt tacaagattt atgacatcta tgcggctact gcagcgctga
    56341 tcgtcgccac ggcgattgtg cttatctata gctgggttcg ctttcgtaag gttgagaaga
    56401 tggccctgat cacttttgtt ctggtggtcg tcttcggtgg cttgacgctg ttcttccaca
    56461 atgatgagtt tattaaatgg aaggtgacag tcatttatgc tctgtttgcg ggagccctgt
    56521 tagtcagcca atgggtgatg aaaaagccgc taattcagcg gatgctgagt aaagaactca
    56581 cgctgccgca accggtatgg tcgaagctaa atctggcctg ggctgttttc tttatccttt
    56641 gcggtctggc aaacatctac atcgcattct ggctgccgca aaatatttgg gtcaacttta
    56701 aagtctttgg cctgaccgcc cttaccttaa tcttcacatt gttaagcggt atctatatct
    56761 accgccacat gccgcaggaa gataaatcct aaccagactg ccagtccttc ggggctggcg
    56821 ctgtatctcc tctgttctcc tcaggaaaat catagtagca tcgcgcctgt gattttcctt
    56881 ttaagtcggt tttaccatgt ctacaacaca taacgtccct cagggcgatc ttgttttacg
    56941 tactttagcc atgcccgccg ataccaatgc caatggtgac atctttggtg gttggttaat
    57001 gtcacaaatg gatattggcg gcgctattct ggcaaaagaa attgcccacg gtcgcgtagt
    57061 gactgtgcgg gttgaaggaa tgactttctt acggccggtt gcggtcggcg atgtggtgtg
    57121 ctgctatgca cgctgtgtcc agaaagggac gacatcggtc agcattaata ttgaagtatg
    57181 ggtgaaaaaa gtagcgtctg aaccaatcgg gcaacgctat aaagcgacag aagcattatt
    57241 taagtatgta gcggttgatc ctgaaggaaa acctcgcgcc ttacctgttg agtaatagtc
    57301 aaaagcctcc ggtcggaggc ttttgacttt ctgcttactg aatttcggtg gtgccgttaa
    57361 ttttaaacag gatattcacc acaatcccac tgcctggctt acccggctca taacgccatc
    57421 tgcgcatcgc atttttcacc tcacgctcaa acatgttcgc aggcttggct gagaggattt
    57481 gtacgttatc cacgcgacca tccggcgtga cgtcaaattt aactttaacc tgcccttcaa
    57541 tgcgcaatgc ctgtgctcgt gccggatact gcggctgatt acggcttaat gcgcgtggtc
    57601 ctgaagccac actggtaacc ggcttgctgg ttgcagccgt tgccgtactt gatgtggggc
    57661 gcgcgggtgc cgtattttca aacggtgatg ccggacgcga ctctacgggt ttgacatcgc
    57721 gttttggctg ctcctgtacc tttttcaccg gcttcggttt tggcttcggt ttcggcttcg
    57781 gcttttcaat gaccaccggt gcttcttttg gcggttcggg gatcggctca ggttccggtt
    57841 ctggctctac caccggctcc ggtggcggct gaacggcttg tggcggttcg agatcagcag
    57901 gcgcaaccat cgtgacagaa attggttgcg caggcgcagg tagttcaata acctgatgta
    57961 ccgaggtata gagcagcccc gccacaacag caccatgaat gcagaccgaa agtaacgt
//
LOCUS       DAJTNV010000014        57725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNV010000014 DAJTNV010000000
VERSION     DAJTNV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            700..1128
                     /gene="uspC"
                     /locus_tag="OO199_000842"
     CDS             700..1128
                     /gene="uspC"
                     /locus_tag="OO199_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5534993.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1135..2549)
                     /gene="otsA"
                     /locus_tag="OO199_000843"
                     /pseudo
     CDS             complement(1135..2549)
                     /gene="otsA"
                     /locus_tag="OO199_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2524..3324)
                     /gene="otsB"
                     /locus_tag="OO199_000844"
     CDS             complement(2524..3324)
                     /gene="otsB"
                     /locus_tag="OO199_000844"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5534994.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3491..4477)
                     /gene="araH"
                     /locus_tag="OO199_000845"
     CDS             complement(3491..4477)
                     /gene="araH"
                     /locus_tag="OO199_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5534995.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4492..6006)
                     /gene="araG"
                     /locus_tag="OO199_000846"
     CDS             complement(4492..6006)
                     /gene="araG"
                     /locus_tag="OO199_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5534996.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6076..7065)
                     /gene="araF"
                     /locus_tag="OO199_000847"
                     /pseudo
     CDS             complement(6076..7065)
                     /gene="araF"
                     /locus_tag="OO199_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7862..8365
                     /locus_tag="OO199_000848"
     CDS             7862..8365
                     /locus_tag="OO199_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5534997.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8445..8696)
                     /locus_tag="OO199_000849"
     CDS             complement(8445..8696)
                     /locus_tag="OO199_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5534998.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8811..8897)
                     /gene="azuC"
                     /locus_tag="OO199_000850"
     CDS             complement(8811..8897)
                     /gene="azuC"
                     /locus_tag="OO199_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5534999.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9160..9483
                     /locus_tag="OO199_000851"
     CDS             9160..9483
                     /locus_tag="OO199_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5535000.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9654..10151
                     /gene="ftnA"
                     /locus_tag="OO199_000852"
     CDS             9654..10151
                     /gene="ftnA"
                     /locus_tag="OO199_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5535001.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10188..10427)
                     /locus_tag="OO199_000853"
     CDS             complement(10188..10427)
                     /locus_tag="OO199_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5535002.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10617..11828
                     /gene="tyrP"
                     /locus_tag="OO199_000854"
     CDS             10617..11828
                     /gene="tyrP"
                     /locus_tag="OO199_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5535003.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11890..12555)
                     /gene="yecA"
                     /locus_tag="OO199_000855"
     CDS             complement(11890..12555)
                     /gene="yecA"
                     /locus_tag="OO199_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5535004.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12751..12837)
                     /locus_tag="OO199_000856"
     tRNA            complement(12751..12837)
                     /locus_tag="OO199_000856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12801..12803),aa:Leu,seq:taa)
     gene            complement(12850..12923)
                     /locus_tag="OO199_000857"
     tRNA            complement(12850..12923)
                     /locus_tag="OO199_000857"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12889..12891),aa:Cys,seq:gca)
     gene            complement(12978..13053)
                     /locus_tag="OO199_000858"
     tRNA            complement(12978..13053)
                     /locus_tag="OO199_000858"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13018..13020),aa:Gly,seq:gcc)
     gene            complement(13205..13753)
                     /gene="pgsA"
                     /locus_tag="OO199_000859"
     CDS             complement(13205..13753)
                     /gene="pgsA"
                     /locus_tag="OO199_000859"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5535005.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13810..15642)
                     /gene="uvrC"
                     /locus_tag="OO199_000860"
     CDS             complement(13810..15642)
                     /gene="uvrC"
                     /locus_tag="OO199_000860"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5535006.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15639..16295)
                     /gene="uvrY"
                     /locus_tag="OO199_000861"
     CDS             complement(15639..16295)
                     /gene="uvrY"
                     /locus_tag="OO199_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5535007.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16591..16767
                     /locus_tag="OO199_000862"
     CDS             16591..16767
                     /locus_tag="OO199_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535008.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16754..16978
                     /locus_tag="OO199_000863"
     CDS             16754..16978
                     /locus_tag="OO199_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5535009.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17011..17631)
                     /gene="sdiA"
                     /locus_tag="OO199_000864"
                     /pseudo
     CDS             complement(17011..17631)
                     /gene="sdiA"
                     /locus_tag="OO199_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17861..18613)
                     /gene="yecC"
                     /locus_tag="OO199_000865"
     CDS             complement(17861..18613)
                     /gene="yecC"
                     /locus_tag="OO199_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5535010.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18610..19278)
                     /gene="tcyL"
                     /locus_tag="OO199_000866"
     CDS             complement(18610..19278)
                     /gene="tcyL"
                     /locus_tag="OO199_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5535011.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19293..20279)
                     /gene="dcyD"
                     /locus_tag="OO199_000867"
     CDS             complement(19293..20279)
                     /gene="dcyD"
                     /locus_tag="OO199_000867"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5535012.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20384..21184)
                     /gene="tcyJ"
                     /locus_tag="OO199_000868"
     CDS             complement(20384..21184)
                     /gene="tcyJ"
                     /locus_tag="OO199_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5535013.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21272..21823)
                     /gene="fliZ"
                     /locus_tag="OO199_000869"
     CDS             complement(21272..21823)
                     /gene="fliZ"
                     /locus_tag="OO199_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5535014.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21869..22588)
                     /gene="fliA"
                     /locus_tag="OO199_000870"
     CDS             complement(21869..22588)
                     /gene="fliA"
                     /locus_tag="OO199_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5535015.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22753..24405)
                     /gene="fliC"
                     /locus_tag="OO199_000871"
     CDS             complement(22753..24405)
                     /gene="fliC"
                     /locus_tag="OO199_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5535016.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24671..26077
                     /gene="fliD"
                     /locus_tag="OO199_000872"
     CDS             24671..26077
                     /gene="fliD"
                     /locus_tag="OO199_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5535017.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26102..26512
                     /gene="fliS"
                     /locus_tag="OO199_000873"
     CDS             26102..26512
                     /gene="fliS"
                     /locus_tag="OO199_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5535018.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26512..26877
                     /gene="fliT"
                     /locus_tag="OO199_000874"
     CDS             26512..26877
                     /gene="fliT"
                     /locus_tag="OO199_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5535019.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26955..28442
                     /gene="amyA"
                     /locus_tag="OO199_000875"
     CDS             26955..28442
                     /gene="amyA"
                     /locus_tag="OO199_000875"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5535020.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28476..28889)
                     /locus_tag="OO199_000876"
     CDS             complement(28476..28889)
                     /locus_tag="OO199_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5535021.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29076..30281
                     /gene="yedE"
                     /locus_tag="OO199_000877"
     CDS             29076..30281
                     /gene="yedE"
                     /locus_tag="OO199_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5535022.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30278..30511
                     /gene="yedF"
                     /locus_tag="OO199_000878"
     CDS             30278..30511
                     /gene="yedF"
                     /locus_tag="OO199_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5535023.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30618..31288
                     /locus_tag="OO199_000879"
                     /pseudo
     CDS             30618..31288
                     /locus_tag="OO199_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31328..32536)
                     /locus_tag="OO199_000880"
                     /pseudo
     CDS             complement(31328..32536)
                     /locus_tag="OO199_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32604..33219
                     /gene="tnpA"
                     /locus_tag="OO199_000881"
                     /pseudo
     CDS             32604..33219
                     /gene="tnpA"
                     /locus_tag="OO199_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33547..34629
                     /locus_tag="OO199_000882"
     CDS             33547..34629
                     /locus_tag="OO199_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5535024.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35118..35916)
                     /locus_tag="OO199_000883"
                     /pseudo
     CDS             complement(35118..35916)
                     /locus_tag="OO199_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35926..36477)
                     /gene="ybcL"
                     /locus_tag="OO199_000884"
     CDS             complement(35926..36477)
                     /gene="ybcL"
                     /locus_tag="OO199_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5535025.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36646..36978)
                     /gene="emrE"
                     /locus_tag="OO199_000885"
     CDS             complement(36646..36978)
                     /gene="emrE"
                     /locus_tag="OO199_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5535026.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37323..37637)
                     /gene="fliE"
                     /locus_tag="OO199_000886"
     CDS             complement(37323..37637)
                     /gene="fliE"
                     /locus_tag="OO199_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5535027.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37851..39516
                     /gene="fliF"
                     /locus_tag="OO199_000887"
                     /pseudo
     CDS             37851..39516
                     /gene="fliF"
                     /locus_tag="OO199_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39509..40504
                     /gene="fliG"
                     /locus_tag="OO199_000888"
     CDS             39509..40504
                     /gene="fliG"
                     /locus_tag="OO199_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5535028.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40497..41183
                     /gene="fliH"
                     /locus_tag="OO199_000889"
     CDS             40497..41183
                     /gene="fliH"
                     /locus_tag="OO199_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5535029.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41183..42556
                     /gene="fliI"
                     /locus_tag="OO199_000890"
     CDS             41183..42556
                     /gene="fliI"
                     /locus_tag="OO199_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5535030.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            42578..42919
                     /gene="fliJ"
                     /locus_tag="OO199_000891"
                     /pseudo
     CDS             42578..42919
                     /gene="fliJ"
                     /locus_tag="OO199_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42916..44043
                     /gene="fliK"
                     /locus_tag="OO199_000892"
     CDS             42916..44043
                     /gene="fliK"
                     /locus_tag="OO199_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5535031.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44148..44612
                     /gene="fliL"
                     /locus_tag="OO199_000893"
     CDS             44148..44612
                     /gene="fliL"
                     /locus_tag="OO199_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5535032.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44617..45621
                     /gene="fliM"
                     /locus_tag="OO199_000894"
     CDS             44617..45621
                     /gene="fliM"
                     /locus_tag="OO199_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5535033.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45618..46031
                     /gene="fliN"
                     /locus_tag="OO199_000895"
     CDS             45618..46031
                     /gene="fliN"
                     /locus_tag="OO199_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5535034.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46034..46399
                     /gene="fliO"
                     /locus_tag="OO199_000896"
     CDS             46034..46399
                     /gene="fliO"
                     /locus_tag="OO199_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5535035.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46399..47136
                     /gene="fliP"
                     /locus_tag="OO199_000897"
     CDS             46399..47136
                     /gene="fliP"
                     /locus_tag="OO199_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5535036.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47146..47415
                     /gene="fliQ"
                     /locus_tag="OO199_000898"
     CDS             47146..47415
                     /gene="fliQ"
                     /locus_tag="OO199_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5535037.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47424..48209
                     /gene="fliR"
                     /locus_tag="OO199_000899"
     CDS             47424..48209
                     /gene="fliR"
                     /locus_tag="OO199_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5535038.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48494..49117
                     /gene="rcsA"
                     /locus_tag="OO199_000900"
     CDS             48494..49117
                     /gene="rcsA"
                     /locus_tag="OO199_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5535039.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49161..49349)
                     /gene="dsrB"
                     /locus_tag="OO199_000901"
     CDS             complement(49161..49349)
                     /gene="dsrB"
                     /locus_tag="OO199_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5535040.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49512..49739
                     /gene="yodD"
                     /locus_tag="OO199_000902"
     CDS             49512..49739
                     /gene="yodD"
                     /locus_tag="OO199_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5535041.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50037..50852
                     /locus_tag="OO199_000903"
                     /pseudo
     CDS             50037..50852
                     /locus_tag="OO199_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50849..52543)
                     /gene="yedQ"
                     /locus_tag="OO199_000904"
                     /pseudo
     CDS             complement(50849..52543)
                     /gene="yedQ"
                     /locus_tag="OO199_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52714..52896)
                     /locus_tag="OO199_000905"
     CDS             complement(52714..52896)
                     /locus_tag="OO199_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5535042.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52975..53892)
                     /locus_tag="OO199_000906"
     CDS             complement(52975..53892)
                     /locus_tag="OO199_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5535043.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54065..54985
                     /gene="yedA"
                     /locus_tag="OO199_000907"
     CDS             54065..54985
                     /gene="yedA"
                     /locus_tag="OO199_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5535044.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54974..55444)
                     /gene="vsr"
                     /locus_tag="OO199_000908"
     CDS             complement(54974..55444)
                     /gene="vsr"
                     /locus_tag="OO199_000908"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5535045.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55425..56843)
                     /gene="dcm"
                     /locus_tag="OO199_000909"
     CDS             complement(55425..56843)
                     /gene="dcm"
                     /locus_tag="OO199_000909"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5535046.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56910..57450)
                     /locus_tag="OO199_000910"
                     /pseudo
     CDS             complement(56910..57450)
                     /locus_tag="OO199_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57490..>57725)
                     /locus_tag="OO199_000911"
     CDS             complement(57490..>57725)
                     /locus_tag="OO199_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535047.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        14517 a        14771 c        13898 g        14539 t
ORIGIN      
        1 aattagcatt agaatagttg cgataagctg caataagcag agccaccttt ttggtttaat
       61 aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc
      121 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg
      181 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
      241 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
      301 aggaataatc gaaatgggat gttgcgcacg gttaaaataa ctcaccgtaa ataatcatct
      361 gctataaata atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg
      421 cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta ataaaatgtt
      481 tttcatatat taaccacttg tttgatgcgg ggaatcagta cattattttc ttataattac
      541 agttgaaatc atatgatcac cgagagtgat tgattaattg tctttactaa taatcagact
      601 aatatttacc tgtttgactg agttgggatt gcgtcgtttc tccattagga gtaaagcttt
      661 aatgtcacct gaagttcaca gcagaataag gaacaggtta tgagctatag caatattctt
      721 gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt atctatcgcc
      781 aggccagtaa agggacacat cagtttaatt actctcgctt ccaacccgga aatgtacaat
      841 caattagccg cgccgatgct tgaagattta cgcagcgtga tgcaagaaga aacgcaaagc
      901 tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt tattgcctac
      961 ggtgagttaa gcgaacatat tctggaagta tgtcacaaac atcatttcaa tttggtgatt
     1021 tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa aagagtgatt
     1081 gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gagattaacc gctcctacgc
     1141 aagctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc gcggaactat
     1201 ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt tcacgataac
     1261 gtccagcatt tctgcatgac gggaaatacg ttccaccagc gacatagtca atgcacgatc
     1321 cagcgcagct gcaacttcgt cacgatcgta ggggttaaca attaacgccg acgttaactc
     1381 gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc gggttggctg ggtcctgagc
     1441 agcaacatac tcttttgcta ccaggttcat cccgtcacgc agtggcgtca ctaagcccac
     1501 gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat tcaaataata
     1561 aagcggcgtc cagcctaatt gcccgtattt accattaatt cgtccagctt cattttcgag
     1621 ctgatgacga atatcctggt aggcttgcac atcaccacgc gacgttggtg caatctgggt
     1681 ataacgaatt ttaccatgat gctgcggata tttttccagc aatgcctcat aggcgagaaa
     1741 acgctccggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
     1801 tttcaattcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
     1861 tatttctttc ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt ttccccaggc
     1921 tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg ttagaaaggc aatccaggaa
     1981 cgccagacga tcgttttctg tctgtaaacc cagcaaatca taatcacaaa gctgttcaag
     2041 caaggtgtca tatgtcggca gcgcgttgaa aatttccggt gtcgggaaag gaatatgcaa
     2101 gaaaaaacca atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag
     2161 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
     2221 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
     2281 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
     2341 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga
     2401 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc tttcagtgcc
     2461 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
     2521 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
     2581 ttgtaatgcg gcggttatca tttcaagcca gctccagaca tccggcacac ccgccagtcg
     2641 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     2701 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     2761 aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac ctctcggttt
     2821 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     2881 ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     2941 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg tatgcagttg
     3001 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt taccattgat
     3061 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     3121 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     3181 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     3241 gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg atagttcagg
     3301 ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta ttgtgttgcg
     3361 cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc catttgcaac
     3421 gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta aattgttgtg
     3481 gaaaaaagca tcagacagtg cgcttcgctt tttgcttgta acggtcgaag atcaccgctg
     3541 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     3601 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     3661 tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata agctcataac
     3721 caatcgacgt cattggctgc ccactagtca tacgtgaagc cagaataatt ccggctatcg
     3781 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtatacccg
     3841 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt attacgacca aaggtggttt
     3901 tattcagcag caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca
     3961 gaccgaacca gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt
     4021 taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgtcgtga
     4081 tcagagcatt tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaacgc
     4141 ccagcaacaa acccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg
     4201 ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag
     4261 caaggcagaa caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc
     4321 ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca
     4381 ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact
     4441 taggtgcgcc agaccccgat gtagaaacag aagacatcat actctcctta ctcaggcaac
     4501 tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg
     4561 taacaattca ccggcgattt cgccttcccg catcaccaca atccggtcgg caacgccgag
     4621 gacttcaggt aagtcgctgg aggcaaacag caccgccacg ccctgcgccg ccagcgcata
     4681 aattacgtta tatatttcgt gcttagcgcc aacatcaatg ccgcgcgtag gttcatccag
     4741 caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc
     4801 gcctgagaga ttcatgatca gttgctccgc gcccggcgtt ttgatgttga gcgaacgaat
     4861 gtgctgatcg gcattgtttt cttcccaact gttgttgatt acacaaccgc cgagcacatg
     4921 tttgcgtctg gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc
     4981 cgctttgcga gcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg
     5041 aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa
     5101 caagcctttc attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc
     5161 accactgcga accgccagac ttattggcgt acgcacgcct agtgctttca cagcatcaag
     5221 acgcaggcgc tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg
     5281 cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt
     5341 gacataacgt ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat
     5401 acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa
     5461 aagattgccg atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt
     5521 agcgttacgc gccagcgctt tggcgatttc aaccatctgc cactgaccaa tggcgagata
     5581 tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc
     5641 ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat
     5701 gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc
     5761 cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga
     5821 acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc
     5881 attttcaccc atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag
     5941 cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg
     6001 ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg
     6061 aatttttccg gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt
     6121 gtcacgcgtg atcagtacca cgtctgtaac ttcggtaaat tttggcgttt caacgtcttt
     6181 tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag
     6241 cagggaaccg tagaagccgg ttgcctgtgc tttagacagt tcgcttactg catccacacc
     6301 gttaatacca ataccgatga tatcggctgc tttaaagccc tgaccttcgg ttgcacgtac
     6361 gccgccaagt acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttccggatg
     6421 ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg
     6481 tacctgataa atttgttttt ccgggaatcc tgcagctttc agtgcgtcca tagaacccgt
     6541 tgtacggcgg cgggcggtat ccagttcgtt ggcggtaatc gccatcactg cgctttcttt
     6601 gacatcccag ccacgtttct gcatctcttt atacagttcc tagccctgac gttcgccaat
     6661 tttagtcgcc gccatcatca ccagaggaac ggtatccatt ggcttacctt tggcgttaac
     6721 aaactggtca tccactgcaa tgactttcat atcgtagcca cgcgctttcg cgacgatggc
     6781 agagccgagt ttcgggtccg gagtacaaat aacgaaacct tttgcgccac tggctgccag
     6841 gctgtcgatc gcgttcaatg ttttttcgcc atccggcacg gcaatcttaa taacctcaaa
     6901 ccctaaatcc ttcccggctt tatcggcaaa cttccattcg gtctggaacc acggctcttc
     6961 cggttgcttc accagaaaac cgagcttcag gttctccgcc atagcggatt gtgacataac
     7021 ggctgccaga ccaatggctg ccagggcttt agtaaatttg tgcatggttc tctccagctt
     7081 tagtgtcgtt ttgtgtagtg caaaaacgaa tgacattcgt taaattaatc ggaaaacaaa
     7141 gcattacctt ttatctaaaa gataagtgac cgtgttgaca tagttttagc gagaaattaa
     7201 ttctccatag gagagcaata tcacatcgca gaattacagt gagaacgtgc ataaatttag
     7261 cgggaaaaga cataaggaaa agccaatttg tcagacaaat tgttgaatgc acagcagatt
     7321 aatccataag attagcctgg aaatccttgt tgtcttcggt acccattcgg gatgtcttct
     7381 ttttaaccag tcaataggcc gcattacctg gcgttgagtt tttgaaatgg tgtaataacc
     7441 acaactcaaa gatgtggaaa atgcgcgtca ttcatttcgc cattaattat cactgtgctc
     7501 attaattaac agaacacgta taatgagagc catctcgcaa aaatgaaaaa acgttttata
     7561 aaatcatcac ttcatcatga attcaaattc atcgattaat atcaacaaga tacaaaaagc
     7621 actatcatta aaattcattg catttacatt gatttcacca atgaaatgta aaaatatata
     7681 aacttgatga tttaagcatt ttcttataac cgttcagacg ttattcttat ttcagatcat
     7741 cgtcagaatt gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca
     7801 gtaattatgg tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg
     7861 tatggcaacc gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc
     7921 atccaatctc taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc
     7981 cactttcctt cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt
     8041 tatgaagagt gtcggcgcta cccccatcgt taaagccatt gatgttccag gtgaaaaact
     8101 gaactctctg gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttcaagcac
     8161 tctggcgcag ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct
     8221 gcgcgatctg gaaaaagaac agcagcatga tggcctgttg ctgcaaacca ttcttgatga
     8281 agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa
     8341 tgtcgtgtca caccagctgc attgatcata catcggcgct aatacattgc gccgatgaag
     8401 tttttgagaa accgctgcct catctgtttg aagcagcggt ttttttaatg ggattcaccc
     8461 tgtggggtaa acttgagttc aatcagcgcg atggcttttt ggattgcccg catggtgacc
     8521 gggtctgcgg cagcgggatg atgagtaaag tcgatattct tcagctgact ggacattttt
     8581 tcacgaactt caacgggcgc gattacatcg agaacatcca gaatttgttt gataaccaac
     8641 tggcaagcta ccacatcaga aaccagttcc tgatcggcat tcagcggctg ggacatcgta
     8701 aactcctgat agcattttga aagccgttat agtagcggct tcacatcttc agcgatagtc
     8761 acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg ttatcggaac
     8821 atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat acttttcagg
     8881 attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt attgtctttg
     8941 cgcttataat atgacaacca tcacaaaaat caatctttat gtgatacaaa tcacataaat
     9001 acccctttaa tgttataaaa atgataatca aaaaacagcc cccctatttc tgacacctac
     9061 agatggcaag aaatagcgcc tgccaggcgt cttttccggc cattgtcgca gcgctgtaac
     9121 gcgtaaaata gtgctttctc ttactcttct ggctggacca tgagacttct gattctgact
     9181 ctttcattaa taacgctggc ggggtgtacg gttactcgcc aggcccatgt gagcgaagtt
     9241 gacgcggcaa caggtattgt acggttggtt tatgaccagg cttttttgca gcatgcccat
     9301 accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat gccagcagga aggctatacc
     9361 cacgcgatcc cctttggtca gccagtaggc aactgtagcc tttttgcggg ttccttatgt
     9421 ttgaatacgg aattcacttt gtcatatcag tgccatcatt ccgccttccc tgtctttctc
     9481 taaatatttt aatcagcgag gggatcttcg ctgattaaag aaatagcggt aatgcgtttt
     9541 attcccattc gtatttttaa taattgaagt ttatatttta ccttttgcaa ataataaaat
     9601 aacaaattat agtgacgcca cagcaacaaa tataaccttt gtggagcact atcatgctga
     9661 aaccagaaat gattgaaaaa cttaatgagc agatgaacct ggaactgtac tcttcactgc
     9721 tttatcagca aatgagcgcc tggtgcagct atcatacctt cgaaggtgct gccgcgttcc
     9781 tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg tctgtttgat tacctgactg
     9841 ataccggcaa tttaccgcgt attaataccg ttgaatctcc gtttgctgaa tattcctcac
     9901 ttgatgaatt attccaggaa acctataaac acgaacaatt aatcacccag aaaattaacg
     9961 aactggctca tgctgcaatg accaatcagg actacccaac atttaatttc ctgcagtggt
    10021 atgtttctga gcagcatgaa gaagagaaac tgttcaaatc gattattgat aaattaagcc
    10081 tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa agaactctct accctcgaca
    10141 cacaaaacta atgctctccg gcggcaggtt tctgccgccg tttcaaatca gtgacggcaa
    10201 atcttactct gatcggtcga aaaaccttct tctgaaggta taaatttgcc ttttgctgcc
    10261 agaaacgcta ccagctctcc cgctgtcacc ccttctgccg agcacgtgtg aaaacgtgcc
    10321 tgttcaccaa aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct
    10381 gattcaatca tcatatttaa cacttcatga ccgtgaatag agtccatcgt tcctcctcaa
    10441 aaaaagccta gcgtagcgat taccgcttat gaagactttg cgccagcgca ggactgaatg
    10501 cttttattgt acatttatat ttacacaata tgtaacgtcg gtttgacgaa gcagccgtta
    10561 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
    10621 aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
    10681 tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg
    10741 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
    10801 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
    10861 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcagcatac atcagcggtg
    10921 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
    10981 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg
    11041 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
    11101 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
    11161 tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga
    11221 gtattgtcag ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta
    11281 gtgccatccc cctggtggca tatattttct ggcaggtggc gacgcttggc agcattgatt
    11341 caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt
    11401 tacgcgaaat ggtggcctct ccgcatgttg agctggcggt gcatttattt gctgatttag
    11461 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttgt
    11521 ttcagcgttc aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc
    11581 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
    11641 tggcgctggc ggtactggca ttgattatcc cttcactatt gacctggcaa agcagaaagc
    11701 acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc
    11761 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcaggcttg ttaccagaag
    11821 tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
    11881 tgtgacctct taatgcaggc agcactgctt aaatttctta ccactgccgc acggacaagg
    11941 atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
    12001 atgcgccatc cagtatgcat gtaaatcaag cgccgccagt cgaatggcat ctacgctctc
    12061 ttcaaacgct tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag
    12121 cgcaatcgcc tccagcgctg gttttaaaga atcgggcaac gttgaccagt cagaaagtgc
    12181 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
    12241 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
    12301 atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca
    12361 acgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttctg gttcaatctc
    12421 ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc
    12481 gtggtcagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
    12541 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
    12601 gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg
    12661 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
    12721 attactttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
    12781 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
    12841 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
    12901 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac
    12961 gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgagaccc cgaccttggc
    13021 aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt
    13081 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
    13141 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa
    13201 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
    13261 gagtcagtac cgcagccaca aagaaaagtg caataccgac gtactcaacc caaatgttcg
    13321 gacgccacag cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc
    13381 aggagacggc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
    13441 taataatttc acgggcgatc atcgttgccg ccggtaatgt cacccaccag ctgtgataat
    13501 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgccaca gggtcaagga
    13561 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
    13621 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
    13681 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
    13741 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
    13801 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
    13861 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
    13921 tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt
    13981 atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc
    14041 gcgaatatgc tggataacat gcagcgcggg tgaatctggc ggcaaactaa atccctcacc
    14101 ttccggctca aagaacagcg tttccagtcc agccttacga tctgctcctt tggcaacgcc
    14161 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
    14221 ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
    14281 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
    14341 cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
    14401 caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat
    14461 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt
    14521 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
    14581 ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
    14641 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
    14701 cggtaaggtg cgcatctggc tgccttgtaa atagaactga cctacgaagg tttctaccac
    14761 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
    14821 gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat
    14881 aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc
    14941 ctgaatttgg tcgcgaatac atgccgcttc ttcaaactcc agattctggc tggcagtttc
    15001 catacggcta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
    15061 atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag
    15121 acagcgccct atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc
    15181 gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc
    15241 attcgggaac gggccgaaat attcaccttt ggcatgcttt gcaccacgat gcatcgccag
    15301 acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa
    15361 aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt
    15421 ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt
    15481 gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
    15541 gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt
    15601 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
    15661 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
    15721 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
    15781 tctgagatct cattgacctt ctggccctta gtgatcatca gcataatctg caattcacgt
    15841 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
    15901 tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
    15961 acgacttcct gcggagccgc gcctttgctg aggtagccag cagcacctgc ctgcatgact
    16021 ttcgctggta aagggttttc tgtatggacc gtaagcatga tgattttaac atcagctgtg
    16081 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
    16141 agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc
    16201 tctccggcga cttttatacc cttaatatct tccagaatgc gtcgtatccc tgcgcgcacc
    16261 agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata
    16321 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
    16381 aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg
    16441 tcacgcccgt caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg
    16501 acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat
    16561 taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt
    16621 aacgtatttt atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca
    16681 aagcttatgg tatactccgc cgccttaaca tttttcacca caaattttca ttgcaacatg
    16741 acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc
    16801 aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc
    16861 tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac
    16921 ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata
    16981 taaaaccggc tgagagcgtg cctttcagcc ggttattgac aaaatcatcg ctatctctgc
    17041 tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat
    17101 ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac
    17161 cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca
    17221 acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc
    17281 gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc
    17341 atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcacag gatcaattgc
    17401 gagaaaattt tttgcctgat aataactaac ccacgactca gggtaattgg tgtagaaagc
    17461 cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc
    17521 cagctgctga gcctgaagct ctatttcatg gtaggcctct tctgcggcct ccatcctctg
    17581 aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg
    17641 caacgcccct gagagtgagt atcatttata atgataattc aaactatcac ataaataaga
    17701 tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct
    17761 tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc
    17821 ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc agaagcttct
    17881 cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct
    17941 gctcgactat ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc
    18001 tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt
    18061 tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca
    18121 cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca
    18181 aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag
    18241 cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt
    18301 tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct
    18361 gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga
    18421 tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctacgtagca
    18481 atgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct
    18541 cgaggtcgat accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt
    18601 caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg
    18661 caacgtcgat aacacagtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt
    18721 gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg
    18781 gattgtcgcg gccagcgacg tatcttttac cagactgata aagctgttcg acagcggcgg
    18841 caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat
    18901 accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag
    18961 cgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg
    19021 atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag
    19081 cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg
    19141 cgacaggcgc atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat
    19201 actgagttgc agggtatacc cagccccttt caacaggaac ggcaaggaat caataaccag
    19261 ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc
    19321 gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga
    19381 ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg
    19441 ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag
    19501 ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct
    19561 ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac
    19621 acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg
    19681 gcgtgagttc cggcactgcc cgatgcgact accaccgacg aaatattaac cgccccttca
    19741 cactgttgcg cgatttccag cgcactctcc acataaccta gcgcgccaag agcattagaa
    19801 ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagttct
    19861 tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga
    19921 tccaacaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt
    19981 tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca
    20041 tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct
    20101 gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg ggtgacctca
    20161 tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc
    20221 ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata
    20281 acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata
    20341 aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag
    20401 caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg
    20461 catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct
    20521 gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca
    20581 gcgccgccag acgatcaacg aggatcgcat cgatacgccc tacgcgcaga tcctgatatt
    20641 tggtcgggtc atcatcatag gtacgcacat cgacaccctg aacattctgc cgcagccact
    20701 cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt
    20761 taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg
    20821 tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat
    20881 caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg
    20941 cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt
    21001 tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg
    21061 tgccgctctc tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc
    21121 ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt
    21181 tcatattcac cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca
    21241 caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg
    21301 aggatttctg cacaagtccg gtacaggttt ggcgctgata gtgttgatac ttccgtaatg
    21361 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
    21421 aaccgtcctg cagctgatcg agggaaatat tttgctcatg tagatgatta cccaaacggc
    21481 gcagacgaac gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa
    21541 atttcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
    21601 aaaatcggca caatgccgcc catgattgtt gttccgtttg ccagcctttt tcatcaagca
    21661 gcgtgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga actaaggtaa
    21721 tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa
    21781 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
    21841 caaagttaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
    21901 taatggcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
    21961 tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg
    22021 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta
    22081 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcac
    22141 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcagcatt tgacgataat
    22201 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
    22261 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
    22321 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt
    22381 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacagcatta agtaacccaa
    22441 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
    22501 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
    22561 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
    22621 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
    22681 atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag
    22741 gcattcagac gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt
    22801 tgccagcacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc
    22861 ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt
    22921 gttcaggttg gtgactgcgg aatccaaacg gttttgcacc gcacccaggg aggagcggaa
    22981 tttgtcgatg gagctgatgg cttcatccag agctttcagg ggatcggctg tcgtagctgc
    23041 ttttgtttca gcatctgtcg tcaatttacc atcagcatct ttataaactg ccttaccgct
    23101 gccgttagtc actgaaccat catcctgtag gtaaaaatct acagtatcag atgtggtcaa
    23161 cgcctcgttg ttatcaacat aaaccgaatc aacacctttt gttacagcgc cagttgtgcc
    23221 gttgacgcta taacctgcag tagtgaaatc aacattgttt cccgaggttg atacagcttt
    23281 atcgaaagct gcctgatcaa cttttgcacc tgttactgaa taagtaatag tgtccggcgt
    23341 agtactatta ccggtaaagg agatagaggt accgccaatg tcaatggttg tcgctttggt
    23401 attattggct gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag agccgttttt
    23461 cgttaagcgc ccagttgtat caatgtagag tttatctcca ttgcttgccg taattttacc
    23521 atcactgctg atctgaacat cctgtgcaga accatcgata gtaatagtgc ccttagcggt
    23581 atcaccaact cccggggtca aatatttctg aacatcggca gctgaagccg ttgtggtatc
    23641 aaaagagtat gacttacttg cactgtcata cttgtaattc gttgcactag ccgccgttcc
    23701 gaagccgtta ttaatggttg cctgaacagt atcaccatca ctaactccag ccagcaaatc
    23761 agacgcttta gcagcatcgt aacccgcact cacagtatat ttgttgccga ccactgttgc
    23821 attagcagct accaagtcag ctttagatgc agcagtatta gccacagccc cgccaccatt
    23881 cacattaaac ccattcagcc ccagcgtatc agagtcaatt ttcttcagat caatagtgat
    23941 agtctggccg tcattcgcac caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt
    24001 cacgccgttg aactgggtct gaccggatac gcggtcaatt tcgtcgagac gggatttgat
    24061 ttcgtcctga atggagtcca gatccgaatc agagttagtc ccggtagaag cctgaaccgt
    24121 cagctcacgg atacgctgta agttgttgtt gatttcggac agcgcgcctt cagtggtctg
    24181 tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt taatgttaga
    24241 agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa
    24301 gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt tgatattatt
    24361 ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc gttatcctat
    24421 attgcaagtc gttgattacg ttttgggttt ctacccgtcg gctcaatcgc cgtcaaccct
    24481 gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca ttttttgtta
    24541 gtcgacgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa caattaaacg
    24601 taaactttgc gcaattcagg ccgataaccc cggtattcgt tttacgtgtc gaaagataaa
    24661 aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg atttaagttc
    24721 aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt caaatcagca
    24781 atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt
    24841 ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca gcaccaccag
    24901 cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga aatacaccat
    24961 cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca gagatgatac
    25021 gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg acgacaaaga
    25081 tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat
    25141 caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg gtgaatatcg
    25201 tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct cggtcagcgg
    25261 tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca acggtatgga
    25321 ggtctcggtc gccgcccaga atgcgcagct gacggtcaac aacgtcgcca tcgagaacag
    25381 cagcaacact atcagcgacg cgctggaaaa catcaccctg aacctgaacg atgtcaccac
    25441 gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg cgattaaaga
    25501 ctgggtgaat gcctataact cgctaataga taccttcagc agcctgacca aatacaccgc
    25561 cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg gcgactccac
    25621 gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca gttcttccag
    25681 ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca aactggaact
    25741 ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat
    25801 tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc tgaccagttg
    25861 gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaaa ccctgaataa
    25921 gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa
    25981 agaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca gcagctactt
    26041 aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg ggagacaggt
    26101 catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat
    26161 gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt
    26221 tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa
    26281 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga
    26341 attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa
    26401 tttacgcaac gatgtctccg cagtcgaaga ggtggaagca ttaatgcgta atattgccga
    26461 tgtctggaaa gagtctttac tctccccttc ttcgattcag gacccagtct gatgaacaat
    26521 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
    26581 ctggcaacgg aagaacaatg ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg
    26641 gtgcaggaga tcgcacacct gactgaagag gttgccccgt ccactacgat gcaggagcag
    26701 ctccgcccga tgctgcacct gattctcgac aacgaaagca aggtaaagca gttattacag
    26761 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
    26821 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
    26881 ttaatgagcc gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg
    26941 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
    27001 aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta
    27061 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct
    27121 acgactccta tgatttattt gatctaggtg agtttgatca gaaaggcagc atccctacta
    27181 aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg
    27241 cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
    27301 gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg
    27361 aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg
    27421 atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta
    27481 aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta
    27541 atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga
    27601 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg
    27661 cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta caggaagttg
    27721 cgccaaagcc gctgtttatt gtggctgaat actggtcgca tgaagtcgat aaactgcaaa
    27781 cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat
    27841 tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc
    27901 tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt
    27961 tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
    28021 tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattacg
    28081 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
    28141 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc
    28201 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
    28261 tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
    28321 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgacagac gaaaacggcg
    28381 aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    28441 aaatccatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    28501 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    28561 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    28621 acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat cccccgctgc
    28681 cactgacggc aatccaaggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc
    28741 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
    28801 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    28861 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    28921 gtggcattaa agctctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
    28981 tattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcggctc
    29041 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    29101 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    29161 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
    29221 gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    29281 tccatctgga aggatcgcca ttaacccgca tcgacggcat gatgatcctc ggtatgtttg
    29341 gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc
    29401 gtatccgcat tatgcaggcc atcattggcg gcattatcgc cggttttggc gcgcgtctgg
    29461 caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct
    29521 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
    29581 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
    29641 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
    29701 gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg
    29761 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    29821 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
    29881 ccatcgggat cttcagttac gtacagttag gcgttgaacc taaaatcatg tgggcgggac
    29941 caaatgcggt aattggtggt ttactttttg gttttggcat cgtgctggca ggcggctgcg
    30001 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    30061 gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
    30121 ccaccgactg ggacaaaatc aacctgctga aaacttttgg tccgatggga ggcctgctgg
    30181 tgacatattt gctgctgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct
    30241 tcccccgtga ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
    30301 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
    30361 gccgcaatta aaaaaaggtg agatcctgga agtggtgagc gactgtccgc agtcgatcaa
    30421 taatattcca ctggatgcgc gtaatcacgg ctacacggtg ctggatattc agcaagacgg
    30481 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    30541 gaacatcgtt acctctgaca catagatttt acccgccccc tctcctattc ttttcagtat
    30601 gatctcagga ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc
    30661 ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca
    30721 gatacaatgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc
    30781 atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta
    30841 acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa accgctctgg caacatggtc
    30901 gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc
    30961 ttattttatc tatcgcgctg acggacaacc tgtttttatg gccgcgatag gtagcatacc
    31021 gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg
    31081 tctggtagat attcatgacc gacgcccgct ggttctgtcg ccagaagctg cacgggaatg
    31141 gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc
    31201 agcaaaccag ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg
    31261 cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaatgac gaggacccct
    31321 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg
    31381 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc
    31441 catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca
    31501 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    31561 gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg cggcgcattt
    31621 cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg acattgcgcc
    31681 ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc aaatcctcaa
    31741 tgactatcat tacgtggttt ttgctgacgg tcgttgtgac tttgtgaagg tagtccctgc
    31801 ggatatttgc gatacaggaa tgcagtcgct gtattttgcg tttctgcttc tgccagttgt
    31861 tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcttc ttctggtttt
    31921 tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgaggca agtttagcca
    31981 cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat acttcacttt
    32041 ctgcctgaat actgatgtac cacttaccgc aggactggct gacagtgaca tttttcacaa
    32101 cacccgtgac ctgacggctg ttccggtagc gcatccagcc aagttttggc agaaaaatac
    32161 ggctgttttc ctgagcgagc ttaacgccct gcggatagcg gaatgcatca ttctgtcacc
    32221 gctttttgaa tcaggggaac gccgagcgct tctggaaaaa gtttttgcag gcctgctcag
    32281 ggtctttcag tgactgttgc aatggctgtg acggagcatc tttaatccat tgcgtttcag
    32341 tggcattttt ccactcaacc aatcaggaag ccattttcgc gtaagggatg tctttgttac
    32401 cggccttatg attctcattc tgacgtgcca gagtacgact gaaaacgaaa cgacatgcgc
    32461 ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg tcttaaccgg aatttgaata
    32521 cctgtagtcg tttcacaggg caaattatac tttggtctgt gaaaaaaact gatatccgac
    32581 gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt ctttgttacc aagtaccgac
    32641 gccagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
    32701 cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catctgttaa
    32761 ttaactatcc ccccaaactg gcgatatcca gtctggtaaa caaacagcct caaaggcgta
    32821 tcgggtaggt tactgcgacg ggatcgacca gatattgctg ccaggtatta ccgcaaagat
    32881 gttttgtggc gtcccggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
    32941 cgccaataca ttgaacaaca gcaaacacct ggtcagatga aaaaacgagc cttatatccc
    33001 cgccctgaag gacgggcttt tacggtgcac tggataagcc tggagattat ctacacctgc
    33061 atggacatca ttgtactatg ccccgtacat gccccatcac atcctcaaca tcaaagtatt
    33121 agagccttgc aaaccattat gcgtctcagt ttgtctcatc tatcttacgc aaaaaaaata
    33181 gaactaatat caagcggcgc tatattgcgt attaaataaa accacctcaa ttacattaat
    33241 aaatctaaat aaatatcatg caaataattt ccatggttat ttttaggcta tttaaattaa
    33301 aaaaataaat tatgagtcaa tcataaataa caacctttaa tataatttga ctaccaaatt
    33361 tatattcgtt cacaaaatag tcatgcattg tgtgcaatag aaacagttca gataaaaata
    33421 gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat aatctcattg
    33481 attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca aataaaaggg
    33541 ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt actgatggcg
    33601 atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga tctgtacgga
    33661 aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga cacaacttat
    33721 gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg tttcggtcag
    33781 tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa agataaaacc
    33841 cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta tggccgtaac
    33901 tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt cggtggcgat
    33961 acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc aacctatcgt
    34021 aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta tcagggtaaa
    34081 aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc cactacttat
    34141 gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac taatgatcag
    34201 gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt atgggcagct
    34261 ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga aacccagaac
    34321 atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga agtagttgca
    34381 caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc taaaggaaaa
    34441 gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg tgcaacctat
    34501 tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat tgataagagc
    34561 gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg attggtttac
    34621 cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc caacatataa
    34681 gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta tataaattac
    34741 cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg aacaattaac
    34801 gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta atgtcataag
    34861 catcccttta attatcccct caacctcttg taaatttccc actcttgagc tccacatagc
    34921 ccaaacgccg gatgcaaaaa aaatcacacg tgcatctctg aaattgtgat ggaggaagtg
    34981 agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac cggattgttc
    35041 aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg atgctgttgc
    35101 tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg tgttatatta
    35161 aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt ttccctggca
    35221 atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat ttaacctcac
    35281 gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat gcaatgtgga
    35341 aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc taggttcgga
    35401 tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata aaagtaaaca
    35461 attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt cctgaatttg
    35521 cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata ttaactcggg
    35581 gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagttatcg cataaaattt
    35641 ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat aaagacctgc
    35701 ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta tctgattatt
    35761 atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac ggatacgaca
    35821 taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca tccgtatttc
    35881 ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt ttatctcata
    35941 aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac caactaacgc
    36001 tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata ctttaaactg
    36061 gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg cataaccaaa
    36121 atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc ttcctgcatc
    36181 agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc accatccact
    36241 gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc cttcaggagc
    36301 accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc cagaaaatac
    36361 atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct gaaatgctgc
    36421 ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt ttttcataaa
    36481 aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc attatgaata
    36541 aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa cattagccat
    36601 aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat gcggtgcact
    36661 tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc ctatgatagc
    36721 tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca gaacgatacc
    36781 gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg tctgagctaa
    36841 taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata accgtgtaaa
    36901 cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg cgccaccaag
    36961 ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa ttgtactaca
    37021 gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg ctctggcgtg
    37081 gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa cgaatgagtt
    37141 cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg atcgtggatg
    37201 gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga gtttgcccca
    37261 tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac aggttacaca
    37321 acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt acgcacctga
    37381 atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc gtttagcgcc
    37441 acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt ttgtgtatcg
    37501 cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg ttgcggcagt
    37561 gattcctgcg cacacgcact catcgccgta gcctgtaact ggctgataac cccttcaatc
    37621 ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc ctaccagtaa
    37681 gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc ggatagaaaa
    37741 aacgaaagca caaataatgg gagcgtcaat ttttcgagtt tgctgacccg agagtgagtc
    37801 ttgttccact ttgccaataa cgccgtccat aatcagccac gaggtgcgcg atgaatgcga
    37861 ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat ccgaaaattc
    37921 cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc ctgtgggcga
    37981 aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt ggcgcaattg
    38041 tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc ggcgctattg
    38101 aggttccggc agataaagtt cacgaactgc gcctgcgcct ggcgcaacaa ggtttgccca
    38161 aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa gtttggtatc agccagttca
    38221 gcgaacaggt gaattatcag cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta
    38281 tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc
    38341 gtgaacaaaa atccccttct gcatcggtga cggtaaatct gttacccggc cgcgcactcg
    38401 atgaagggca aattagcgct attgtacatc tggtttccag cgcggttgct ggtctgccgc
    38461 ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt aacccagtcc aataccagcg
    38521 ggcgcgatct taatgacgct cagttgaaat atgccagcga tgtcgaaggc cgtattcagc
    38581 agcgtattga agcgatcctg tcacctattg ttggtaacgg taatattcac gcctaggtca
    38641 cggcgcaact ggacttcgcc agtaaagaac aaacggaaga acagtatcgc cctaacggtg
    38701 atgaatctca tgcggcgctt cgttcacgcc agcttaatga gagcgagcaa agcggttccg
    38761 gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc
    38821 caatcagcac gcctccgaca aatcaaaata accgccagca gcaggcgagc accaccagca
    38881 atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca
    38941 ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga
    39001 attacaaaac cttgccagac ggtaaaccat tgcctctcag caacgaacag atgaagcaaa
    39061 ttgaagctct gacccgcgag gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg
    39121 ttgttaactc gccgttcaat agcagtgacg aaagcggcgg agcactgccg ttttggcaac
    39181 agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg gttgctggtt gctggtactg
    39241 ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag
    39301 gcggtgaaga ctgtgcagca acaggcgcag gcccacgagg aagtggaaga tgcggtggaa
    39361 gtccgtctga gcaaagacga acaactccaa caacggcgcg ctaaccaacg actgggggca
    39421 gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg
    39481 gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt
    39541 catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca
    39601 gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca
    39661 gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgctg cactgaatat
    39721 caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc
    39781 cagcctgctg gaagatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa
    39841 ctttatggag ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc
    39901 caccattctg gtgcatctga agcgcgccca ggccgccgat attctggcgt tgttcgatga
    39961 acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc
    40021 gctggccgaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag
    40081 caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga
    40141 agaagccgtt attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga
    40201 cgagatgttc ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt
    40261 gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg
    40321 cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc
    40381 caaccgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat
    40441 tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc agcggcgagg atacctatgt
    40501 ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca ccggcagagt
    40561 ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc
    40621 agcaactggc gcaactgcaa atgcaggccc acgagcaggg ttatcaggcg gggattgccg
    40681 aaggtcgcca gcaaggtcac gaacagggct atcaggaagg actggcccag gggctggaac
    40741 aaggtctggc agaggcgaag gctcaacaag cgccaattca tgcccggatg cagcaactgg
    40801 tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc
    40861 agatggcgct ggaggcagca cgtcaggtga tcggtcagac accaacggtg gataactcgg
    40921 cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc gggaaaccac
    40981 agctgcgcgt acacccggat gatctgcaac gtgtggatga tatgctcggc gcaactttaa
    41041 gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca ccctggcgga tgtaaagtct
    41101 ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc
    41161 tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga
    41221 taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg
    41281 cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt
    41341 cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa
    41401 cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg
    41461 tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg
    41521 tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt
    41581 ccccgatacg acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac
    41641 accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg
    41701 acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg
    41761 gatgatggca cgctacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg
    41821 gcgcgaagta aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt
    41881 ggtgattgcc gctccggcgg atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc
    41941 cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc
    42001 cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc
    42061 cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg
    42121 taatggcatt agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga
    42181 tgaccagcag gaccccattg ctgactccgc gcgggcaatc ctcgacggtc acattgtgct
    42241 gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag
    42301 tcgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca
    42361 gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg
    42421 cagcgatccg atgctcgata aagccatcgc cctgtggccg cagctggagg gctatttgca
    42481 acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt
    42541 cccgacagtg tcataaccca ggagataacg gcagatggcg gaagaacagc tcaaaatgtt
    42601 gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac
    42661 cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca
    42721 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
    42781 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
    42841 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
    42901 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
    42961 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa
    43021 gcattagcag gcgagacaac aactgacaaa gcggcccccc agttgctggt agcaacagat
    43081 aagcccacga caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg
    43141 gatttactga tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca
    43201 acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca
    43261 aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt
    43321 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta
    43381 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag
    43441 cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag
    43501 agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc
    43561 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg
    43621 ggttctcacg aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg
    43681 caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc
    43741 aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc
    43801 gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
    43861 gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa
    43921 cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt
    43981 ccggttcccg tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc
    44041 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
    44101 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
    44161 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
    44221 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
    44281 aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca
    44341 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag
    44401 caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt
    44461 tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
    44521 agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg
    44581 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
    44641 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
    44701 gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
    44761 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
    44821 ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagccg
    44881 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa
    44941 ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat
    45001 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
    45061 actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc
    45121 tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt
    45181 accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt
    45241 ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg
    45301 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
    45361 aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc
    45421 tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa
    45481 cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggtact
    45541 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
    45601 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
    45661 tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg
    45721 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
    45781 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca
    45841 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
    45901 aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg gtggttgttg
    45961 ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc
    46021 tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga
    46081 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
    46141 ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat
    46201 tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc
    46261 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
    46321 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa
    46381 gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg
    46441 attacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggt
    46501 ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt
    46561 ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg
    46621 cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcactg
    46681 tttttgacct tttttattat gtcgccagtg atcaacaaaa tttatgtcga tgcgtaccag
    46741 ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg
    46801 cgtgagttta tgctgcgtca gacccgcgag gcagatttag ggttgtttgc cagactggcg
    46861 aataccggcc cgttgcaggg gcctgaagct gtgccgatgc gcattttgct cccggcctac
    46921 gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg
    46981 attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttcccccc
    47041 gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg
    47101 ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg
    47161 gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg
    47221 ctggtagcgc tggtcacggg ccttatcatc agtattttgc aggcagccac gcagattaac
    47281 gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat cattattgcc
    47341 ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg
    47401 tatatcatcg ggtagcccgt acaatgatgc aggagacaag cgatcaatgg ctttcctggt
    47461 taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc
    47521 tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg
    47581 ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc
    47641 tggccgtaca gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg
    47701 ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacat
    47761 ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc gcgcatcatg gatatgctgg
    47821 cattactgct gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata
    47881 cctttcacac cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca
    47941 ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc
    48001 tgttgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca
    48061 tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa
    48121 tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat ttgctggctg
    48181 atattattag tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga
    48241 ttatccgaaa aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt
    48301 tttaagctca ctcacatatc gcaacattta ctttaagaca attccaggca aattatacaa
    48361 cactttacgg gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt aggtgaccca
    48421 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    48481 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    48541 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    48601 tgatgacctt gccatagctt gtgattcaca gcgtccttca gtggtgttta ttaatgagga
    48661 ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc
    48721 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    48781 ggtcagaaaa aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat
    48841 ccttggcaat attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt
    48901 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    48961 tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    49021 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    49081 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtgatttccc
    49141 accagtcagg ctgaataaga ttactctgct ttctcaacaa agataccgtc ctgatgccct
    49201 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgcaaccag gtacattgtg
    49261 ccttcactaa actcctcaac tgccagtacc actccaggac gacgcggacc gccatccgtt
    49321 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    49381 gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag tgctgatttt ctcgacggtc
    49441 tatacttaag agatgctagg cggacttaac gactggcggc aacaacagag taacggttgc
    49501 gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac cgcaaaacgt gaggtcagcg
    49561 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    49621 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    49681 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    49741 ttcacaataa aaaaatcccg accctgaggg tgtcgggatg aaacttgctt aagcaagaag
    49801 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ctgcaatttt
    49861 tttcgctgac aagaaaatat tcgcgctatg aatgattaac gctacttatg aatagaaatg
    49921 tgacgcgcgt caccccttgg tggctctggt gtcaacggga tcgctcccga aaaaatcatc
    49981 tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgaccatgt
    50041 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    50101 atagttatga ctggcaaccg gcagccccct ggctcagccg tttacacgaa gccaatattc
    50161 ccgtcattct ctgtagcagt aaaactttag cggaaatgct gtacttgcaa aaaatgttgg
    50221 ggctacaagg cttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat
    50281 ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatctgcc
    50341 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg
    50401 acgatgcaac cattgccgaa tggacgggat taagccgtag ctaggcggcg ctgacgcagc
    50461 tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    50521 tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac
    50581 tggatgcctc tgccggaaaa gatcaggctg ccaattggat tatcgcgacc tatcaacaat
    50641 tgtcaggcag acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcctttac
    50701 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtacatctgc
    50761 atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg
    50821 aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattgc
    50881 gcccggcctg ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat
    50941 cataatcacc ggtttcctcg ctgctactta cccccagcga ggcactgatg cgtatcgttg
    51001 tactcttggc gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct
    51061 ccgcagcctg cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    51121 gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa
    51181 cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga
    51241 cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt
    51301 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    51361 gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga
    51421 ggagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    51481 aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc cagcacaccg tcgaaatggt
    51541 ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt
    51601 ccattgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt
    51661 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    51721 ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgcacggga atactcatcc
    51781 ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt
    51841 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    51901 ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta
    51961 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccagcacgc gagacataca
    52021 tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca
    52081 gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaaa ttcccctcgc
    52141 tgactaatgc atccgatacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt
    52201 gccaggcgtg ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga
    52261 aatccagtgg cgccacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    52321 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctagc
    52381 tggagatata ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa
    52441 gcaataccac gataaagcag agattaacga catgaccggg ccccaggcgg cgtgcgagtt
    52501 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    52561 tgctttttta tgattctggc ataacccggc aatagcgtac cgcaaaacag cgataatttt
    52621 ataatggtgg catgataaga cgcgactatc attcattgca ggcattggga caccaggccg
    52681 gatgtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc
    52741 ctcccccgga acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata
    52801 ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc
    52861 tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa
    52921 acttgttcac cttagttgaa gatggcgaat tttgcataat agccagtgcg agtattaatg
    52981 tgcctgaccg cgcattttcg ctactgcttt cactcccagc accacgatgc cgccgatgat
    53041 aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg
    53101 cccggcgata tgttcaatag cgtgatgcag cggcgcaata ccatgtacca caatcccgcc
    53161 gccgacgagg aacatcgcca gcgtgccgac aatcgataat gctttcatca gccaaggcgc
    53221 gataatcaat aaccctttac ctaatgcctg catcagcgcg ctagattttt ccgccagcca
    53281 ataccctagg tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac
    53341 taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgcct ctgccacaat
    53401 ccccagcgtg atggcgacga tttccgcaga caaaataaaa tcggtacgaa tcgccccttt
    53461 aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg
    53521 gctctgcgct ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac
    53581 cccttcaaag cagagaaacg cgccaccaat catcaacagt ggcgtaatcg cccacgggat
    53641 aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt
    53701 cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc
    53761 attgagcgat aaatcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat
    53821 cacagagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc
    53881 ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg
    53941 cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct
    54001 ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc cgccgtgagg
    54061 gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat
    54121 ttggggctca acctattttg tcattcggat tggcgtggga agctggcctc cgttaatgat
    54181 ggcgggcgtt cgattccagg cagccggtat tttattgctg gcatttttgc tactgcgcgg
    54241 acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct
    54301 ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc
    54361 cgccgtagtg attgcaaccg tgcccctctt taccctgtgc ttcagtcgcc tgtttggcat
    54421 taaaacgcgc aaactggaat gggtgggtat tgccattggg cttgccggaa tcatcatgct
    54481 caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc
    54541 gattagctgg gcgtttggct cagtttatgg ctcgcgcatt accttacctg tagggatgat
    54601 ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta atgatcgcat cgatgattgc
    54661 gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc cttgcggtcg gctatctggc
    54721 gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgta atgtcagtcc
    54781 ggctctcgcc accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg
    54841 actgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc
    54901 ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt
    54961 tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac
    55021 cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc
    55081 gcctacgtaa cgcacactcc cagacaatca gtacgcgcca gccgagctcc tgcaagcgac
    55141 tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcagttc
    55201 gagtcgcagg cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa
    55261 atatcacgca gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct
    55321 gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg
    55381 tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga
    55441 ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt
    55501 tttgccaccg cggcaaagac cggcacgacc accgagttgc cgaactggcg ataggcctga
    55561 gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc
    55621 gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaag
    55681 tctttctcac ccgtggccat atcccaaccg cgatcgatta aaatttccgc gccatctttg
    55741 taataacgag cagacagcgt acgggtgacg ctttgcggat tgttcggata aaccattcca
    55801 taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac
    55861 agcaccggcg tcaggatata tttcgcctca accatcgggt ccagcagctg cgccagcgtc
    55921 actcgctgtg cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagattc
    55981 agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgttta
    56041 ccatcgatga ttttcggatc gtccggcccg ttatcttctg catcggcaac gtcatagccc
    56101 agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg actcttcagg
    56161 tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca
    56221 tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc
    56281 tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg
    56341 tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga
    56401 tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa
    56461 tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt
    56521 ccaccaatcg attcaaaacc acgacggatg ccgccaattc cggcaaatag atcgataaag
    56581 cgaaacgcat aatgcggatg atgttccggt ggtttgggta acaacgtttg cagatgggca
    56641 aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt
    56701 gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcat tttaacatca
    56761 taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg
    56821 gttactgata tattttcctg catagattta accggccatc tgagatggcc gacaggttaa
    56881 cataattact gccgtttagg gccacgcgct cagccagcgg gtgaaaacgc atctatcacc
    56941 ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc catcaaacag
    57001 cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc aggccaatcg cccccagggc
    57061 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    57121 gctgaaactg tgagcggcaa tggcatgaca aacggcctca attttctccg ccggaaattg
    57181 cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg
    57241 ccgttgcgga tgatttttcg ccaggctcac gatatcgtga aaataacagg cggttaaaat
    57301 caccagcata tcgacatcgt catctgccgc cagtttctgt gccgtagccc agacacggcg
    57361 aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga tggtttttca accagttctc
    57421 gaactgtgcc tgccagtgtt gtaactccat acgcgctccg ttgccaaaga gtcgcaacct
    57481 tagcagtttt tattcatagc gtctgcaacg tgcgggacga ggtcgggtgt accatgcaat
    57541 gccgcctaac aaggcaccaa ccagggccat aaacagaaat ccgaccaatg ctcctaacca
    57601 cttccccgcg gtagatccta aatcactttc aaatcccgac acgggggcat cggggatccg
    57661 gctcatcacc cagatgtatc gcgccattgc ccagacgaaa tagccacacc atgtatagaa
    57721 agccc
//
LOCUS       DAJTNV010000015        55420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNV010000015 DAJTNV010000000
VERSION     DAJTNV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO199_000912"
     CDS             complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO199_000912"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5535048.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO199_000913"
     CDS             complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO199_000913"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5535049.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO199_000914"
     CDS             complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO199_000914"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5535050.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3479..4630
                     /gene="argE"
                     /locus_tag="OO199_000915"
     CDS             3479..4630
                     /gene="argE"
                     /locus_tag="OO199_000915"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5535051.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4982..7633
                     /gene="ppc"
                     /locus_tag="OO199_000916"
     CDS             4982..7633
                     /gene="ppc"
                     /locus_tag="OO199_000916"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5535052.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7816..9549
                     /locus_tag="OO199_000917"
     CDS             7816..9549
                     /locus_tag="OO199_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5535053.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9698..10549
                     /locus_tag="OO199_000918"
     CDS             9698..10549
                     /locus_tag="OO199_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5535054.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10536..10877)
                     /locus_tag="OO199_000919"
     CDS             complement(10536..10877)
                     /locus_tag="OO199_000919"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5535055.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10879..11757)
                     /locus_tag="OO199_000920"
     CDS             complement(10879..11757)
                     /locus_tag="OO199_000920"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5535056.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11723..14020)
                     /locus_tag="OO199_000921"
     CDS             complement(11723..14020)
                     /locus_tag="OO199_000921"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5535057.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14071..14391)
                     /locus_tag="OO199_000922"
     CDS             complement(14071..14391)
                     /locus_tag="OO199_000922"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5535058.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14406..15485)
                     /locus_tag="OO199_000923"
     CDS             complement(14406..15485)
                     /locus_tag="OO199_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5535059.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15794..16897
                     /locus_tag="OO199_000924"
     CDS             15794..16897
                     /locus_tag="OO199_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5535060.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16898..18295
                     /gene="ptsP"
                     /locus_tag="OO199_000925"
     CDS             16898..18295
                     /gene="ptsP"
                     /locus_tag="OO199_000925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5535061.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18307..18969
                     /gene="fsa"
                     /locus_tag="OO199_000926"
                     /pseudo
     CDS             18307..18969
                     /gene="fsa"
                     /locus_tag="OO199_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18980..20081
                     /gene="gldA"
                     /locus_tag="OO199_000927"
                     /pseudo
     CDS             18980..20081
                     /gene="gldA"
                     /locus_tag="OO199_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20356..20973
                     /locus_tag="OO199_000928"
     CDS             20356..20973
                     /locus_tag="OO199_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5535062.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO199_000929"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO199_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5535063.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO199_000930"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO199_000930"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5535064.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO199_000931"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO199_000931"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5535065.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO199_000932"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO199_000932"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5535066.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO199_000933"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO199_000933"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5535067.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO199_000934"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO199_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5535068.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO199_000935"
     CDS             30120..30728
                     /locus_tag="OO199_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5535069.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO199_000936"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO199_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5535070.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO199_000937"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO199_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5535071.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO199_000938"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO199_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5535072.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO199_000939"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO199_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5535073.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO199_000940"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO199_000940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5535074.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO199_000941"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO199_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5535075.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO199_000942"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO199_000942"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5535076.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO199_000943"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO199_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5535077.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO199_000944"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO199_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5535078.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO199_000945"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO199_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO199_000946"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO199_000946"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5535079.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO199_000947"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO199_000947"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5535080.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO199_000948"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO199_000948"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5535081.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO199_000949"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO199_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5535082.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO199_000950"
     CDS             complement(44843..45142)
                     /locus_tag="OO199_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5535083.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO199_000951"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO199_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO199_000952"
     CDS             45895..46494
                     /locus_tag="OO199_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5535084.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO199_000953"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO199_000953"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5535085.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO199_000954"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO199_000954"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5535086.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO199_000955"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO199_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5535087.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO199_000956"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO199_000956"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5535088.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO199_000957"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO199_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5535089.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO199_000958"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO199_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5535090.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO199_000959"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO199_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5535091.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO199_000960"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO199_000960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5535092.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO199_000961"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO199_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5535093.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13502 a        15018 c        14158 g        12742 t
ORIGIN      
        1 aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg cactttgtag
       61 gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta accgagcctg
      121 cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg ccgcacgctt
      181 ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca cctgactgaa
      241 tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa tggcttccac
      301 caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg ccaccaggta
      361 atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac aacgcggacg
      421 tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca gccaggtatc
      481 gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag ccagcggcaa
      541 acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc atttaccgcg
      601 aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac cggaagtcac
      661 gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca gatcttcagc
      721 aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca acacatggtc
      781 acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca gccagcctgc
      841 taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc ctaatgggct
      901 gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca gcatctcaac
      961 ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt gagtgtaacc
     1021 tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt gcagctgccg
     1081 gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt cagtcgctac
     1141 ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca ctttgtcgat
     1201 cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga tttgttgtgg
     1261 cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg cctgctcttc
     1321 tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc caacaatatc
     1381 ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga accgttgatc
     1441 tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt attttgaaat
     1501 tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc gcgtacccat
     1561 cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag cgatatctac
     1621 cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta tcatgccgtc
     1681 agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt cggcaatgcg
     1741 ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat ccgcgcccag
     1801 cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt gcccttcgtc
     1861 tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg agttgataag
     1921 cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa gctgggtcac
     1981 tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat gtttcttcgc
     2041 ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa tgtctatctg
     2101 atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca gccctttcat
     2161 cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct gatgtgactc
     2221 acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
     2281 gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
     2341 ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg cctttcagta
     2401 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac ccgatatcgc
     2461 aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
     2521 gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg gtcacacccg
     2581 atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgtgggg
     2641 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgagtaaaga
     2701 cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc ttacgtcctg
     2761 caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca agaagatcgg
     2821 catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct ggcaccgcaa
     2881 tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
     2941 ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gtggcgtcgt
     3001 taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga aactgcggcg
     3061 ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc cctgggctaa
     3121 actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt agctgcggat
     3181 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
     3241 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
     3301 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
     3361 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
     3421 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
     3481 gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca caccttcaat
     3541 aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
     3601 ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa ctcgcaacaa
     3661 atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg cggggcatac
     3721 cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac tgacggagca
     3781 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
     3841 tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca ttctggcgac
     3901 tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
     3961 cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg cacataaagg
     4021 tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg atccagcacg
     4081 cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc aattgcgcga
     4141 taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta cgctcaacct
     4201 cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg agttgcatat
     4261 ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc tcaacgatgc
     4321 attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc tgcatccgcc
     4381 gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg agaaattgct
     4441 cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc aaacgttatg
     4501 cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg atgaatatct
     4561 ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc accatttttg
     4621 ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc gttgccaaac
     4681 tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc tacacctttg
     4741 gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa agcgtcgtga
     4801 atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga
     4861 cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg
     4921 cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa
     4981 tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg
     5041 agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa
     5101 gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc tcaccacctt
     5161 acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa
     5221 cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa
     5281 cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga
     5341 caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg ctcacccaac
     5401 cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gtttaaaaca
     5461 gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc gcctgcgcca
     5521 gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga
     5581 tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg taccgaatta
     5641 cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc ccgtcgaatt
     5701 tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc cgaacgtcac
     5761 tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg atctgttcct
     5821 gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct
     5881 ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg
     5941 ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc
     6001 aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta
     6061 ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct
     6121 gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac
     6181 ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag
     6241 ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct
     6301 tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt
     6361 gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc
     6421 gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcgatgcc
     6481 ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg tcatgaccca
     6541 gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg tgatgattgg
     6601 ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc aatatcaggc
     6661 acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg
     6721 tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc tgtcacaacc
     6781 gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa
     6841 atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg cgattctgga
     6901 agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact
     6961 gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc
     7021 ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg gttcacgtcc
     7081 ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt ggatttttgc
     7141 ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa
     7201 agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt ggccattctt
     7261 ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt ggttggcgga
     7321 atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag agttacgcaa
     7381 cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc atctgatggc
     7441 cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa
     7501 cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc
     7561 ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg caggtatgcg
     7621 taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag ggttttctga
     7681 aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt
     7741 ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat atttttttca
     7801 agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc tagctggaaa
     7861 gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca ggccattatt
     7921 tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt cagctcgctg
     7981 tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc agtgatcggc
     8041 gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta cgggcaggag
     8101 ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc cagcgagtat
     8161 ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac ggcggtggca
     8221 gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg ttatgttgtc
     8281 tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac gtttatcaaa
     8341 aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc tgccgcaccg
     8401 tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct aacaaagtta
     8461 ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc gggtaacgaa
     8521 ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat gagtctgtac
     8581 ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga tccgaatctg
     8641 accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct gcaacaggcg
     8701 ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc gctgatgaac
     8761 atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac gatgaccgcc
     8821 cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta catgaaccag
     8881 caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt ccaggatgtg
     8941 ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac gcatatcaaa
     9001 tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga tcatgttccg
     9061 ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc taacctgtat
     9121 aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc gaacggattc
     9181 ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca caaaactcag
     9241 gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct gctgtggacg
     9301 tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga tcgtaaatac
     9361 agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta cgacggttac
     9421 gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg gattggtaac
     9481 ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga tcaggtgggt
     9541 aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga tactgccgca
     9601 atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt aataatgcca
     9661 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
     9721 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagtaa
     9781 cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa ttgtgctgga
     9841 aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg tgctgtacgt
     9901 tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct ttagcgtgct
     9961 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    10021 tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc tgctacaaac
    10081 gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta tcgtagccag
    10141 cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct cacgcagcca
    10201 ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg cgcttacccg
    10261 cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc tgtttcaaaa
    10321 aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    10381 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    10441 cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt cggaatatcg
    10501 ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag atgggtttgc
    10561 tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat
    10621 gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc ggcaagctcg
    10681 atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc taaacgattc
    10741 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa ctggcacaac
    10801 ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca ggcggttact
    10861 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    10921 ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca tcgaccatgt
    10981 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    11041 cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc ggctgagcgt
    11101 gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca ccagcaaacg
    11161 cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc
    11221 cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta acagcttgct
    11281 tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc gcagtcgctg
    11341 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    11401 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca gagcgtcaag
    11461 gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg catcacgcaa
    11521 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    11581 cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa agaccaccgt
    11641 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    11701 atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    11761 ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    11821 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    11881 actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc agtttgcgta
    11941 atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    12001 acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc ccttgtgcgt
    12061 cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    12121 ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta tacgatcccg
    12181 gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag tagtggcgca
    12241 acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc tcaaagcggt
    12301 taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct tttaataccg
    12361 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    12421 gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta taacgcgcgc
    12481 cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac gagagcagcg
    12541 gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca accatcaggg
    12601 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagcgcag
    12661 cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta aacatcgcga
    12721 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    12781 agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    12841 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt ttcatcagga
    12901 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    12961 tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    13021 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    13081 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agcagttctt
    13141 tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc aacatatact
    13201 ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga atgatgttca
    13261 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc gcgttatggc
    13321 gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg cagtttgccg
    13381 ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct tccagcagta
    13441 acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgtg
    13501 ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata atgtgcccct
    13561 ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct tttacttcat
    13621 ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    13681 cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg
    13741 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccgg
    13801 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    13861 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    13921 tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca cgggtattgg
    13981 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    14041 cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt gagcttcaat
    14101 tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc gcacgatggt
    14161 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa tcaccatatc
    14221 agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc cctgggtttc
    14281 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ctgccatata
    14341 ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca tggctcgttc
    14401 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    14461 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    14521 acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac aatcagacca
    14581 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    14641 ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    14701 tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg ctcttctgcg
    14761 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg gatacaaata
    14821 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    14881 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag ccccatgatc
    14941 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    15001 gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt aatcagtgtg
    15061 ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg caccggaatt
    15121 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc accaaagaag
    15181 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    15241 tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt caaacctgca
    15301 acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg cactgcgcct
    15361 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    15421 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    15481 ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg gaggaaacaa
    15541 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    15601 atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca tgccgcaaaa
    15661 cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    15721 agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag aaaaccgtac
    15781 cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    15841 tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc aaattgagtg
    15901 gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc tgattggcac
    15961 cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg aacaggaagg
    16021 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg cgccgctggc
    16081 ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    16141 aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga cgccgatctc
    16201 ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg acgccgagca
    16261 atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc gtctgctgga
    16321 tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg gcgatacttc
    16381 cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg aagcgattgt
    16441 cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat acctgcaaga
    16501 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    16561 acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg atgaactgac
    16621 ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac tgaaaagcgg
    16681 cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga cgctggttgg
    16741 tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    16801 cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc aagaagcccg
    16861 cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag cccgtaccgc
    16921 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    16981 attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct atatggatcg
    17041 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtctgc
    17101 caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac ccgttgatta
    17161 tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc gtatttatga
    17221 agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct ccgctcacgg
    17281 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    17341 aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg atgagaagat
    17401 ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc aatgctgcga
    17461 agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc tggcagtgga
    17521 tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat tcttgcgggc
    17581 gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc tgtgcggtga
    17641 gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg atgaactcag
    17701 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    17761 gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag tggaacacct
    17821 gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg agtgcatcac
    17881 gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    17941 gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg cgcgcgaggc
    18001 cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat cagaacacat
    18061 tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg gcgatgatga
    18121 agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc agcacatgcg
    18181 cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    18241 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    18301 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    18361 atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    18421 atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat tctgtttgct
    18481 cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct gcgcgacgct
    18541 attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc aattaaaata
    18601 ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc agaagggtta
    18661 ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag
    18721 ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat gcacgcgcca
    18781 gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga ctgtttactg
    18841 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    18901 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    18961 catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat acatccaggg
    19021 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    19081 ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa gctttaaaga
    19141 tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    19201 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta tcggtggcgg
    19261 aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    19321 accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct acaccgatga
    19381 gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca ttgtcgacac
    19441 caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg atgcgctggc
    19501 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    19561 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    19621 cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg agcgcgtgat
    19681 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaag
    19801 tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag gaaatcgaaa
    19861 ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa ctggatatta
    19921 aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca gaaggtgaaa
    19981 ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca ctgctggtag
    20041 ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca aactcccggc
    20101 ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg cctacgcaat
    20161 ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc actccgcatc
    20221 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt tatccgcttc
    20281 tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    20341 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    20401 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    20461 aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt ttacgatccg
    20521 gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg ggtcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccaccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccatacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAJTNV010000016        53895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNV010000016 DAJTNV010000000
VERSION     DAJTNV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO199_000962"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO199_000962"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5535094.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO199_000963"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO199_000963"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5535095.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO199_000964"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO199_000964"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5535096.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO199_000965"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO199_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5535097.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO199_000966"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO199_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5535098.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO199_000967"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO199_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5535099.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO199_000968"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO199_000968"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5535100.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7607..10141
                     /gene="mrcB"
                     /locus_tag="OO199_000969"
     CDS             7607..10141
                     /gene="mrcB"
                     /locus_tag="OO199_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5535101.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10361..12604
                     /gene="fhuA"
                     /locus_tag="OO199_000970"
     CDS             10361..12604
                     /gene="fhuA"
                     /locus_tag="OO199_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5535102.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPVFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12655..13452
                     /gene="fhuC"
                     /locus_tag="OO199_000971"
     CDS             12655..13452
                     /gene="fhuC"
                     /locus_tag="OO199_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5535103.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13452..14342
                     /gene="fhuD"
                     /locus_tag="OO199_000972"
     CDS             13452..14342
                     /gene="fhuD"
                     /locus_tag="OO199_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5535104.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14339..16321
                     /gene="fhuB"
                     /locus_tag="OO199_000973"
     CDS             14339..16321
                     /gene="fhuB"
                     /locus_tag="OO199_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5535105.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO199_000974"
     CDS             complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO199_000974"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5535106.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17861..19282
                     /gene="clcA"
                     /locus_tag="OO199_000975"
     CDS             17861..19282
                     /gene="clcA"
                     /locus_tag="OO199_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5535107.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19306..19371
                     /locus_tag="OO199_000976"
     CDS             19306..19371
                     /locus_tag="OO199_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535108.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19364..19708
                     /gene="erpA"
                     /locus_tag="OO199_000977"
     CDS             19364..19708
                     /gene="erpA"
                     /locus_tag="OO199_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5535109.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19755..20378)
                     /locus_tag="OO199_000978"
     CDS             complement(19755..20378)
                     /locus_tag="OO199_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5535110.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO199_000979"
     CDS             complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO199_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5535111.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO199_000980"
     CDS             complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO199_000980"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5535112.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21991..23508
                     /gene="dgt"
                     /locus_tag="OO199_000981"
     CDS             21991..23508
                     /gene="dgt"
                     /locus_tag="OO199_000981"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5535113.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23638..25062
                     /gene="degP"
                     /locus_tag="OO199_000982"
     CDS             23638..25062
                     /gene="degP"
                     /locus_tag="OO199_000982"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5535114.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25217..26373
                     /gene="cdaR"
                     /locus_tag="OO199_000983"
                     /pseudo
     CDS             25217..26373
                     /gene="cdaR"
                     /locus_tag="OO199_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26462..26848)
                     /locus_tag="OO199_000984"
     CDS             complement(26462..26848)
                     /locus_tag="OO199_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5535115.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO199_000985"
     CDS             complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO199_000985"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5535116.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO199_000986"
     CDS             complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO199_000986"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5535117.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO199_000987"
     CDS             complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO199_000987"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5535118.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31913..32638
                     /gene="rpsB"
                     /locus_tag="OO199_000988"
     CDS             31913..32638
                     /gene="rpsB"
                     /locus_tag="OO199_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5535119.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32896..33747
                     /gene="tsf"
                     /locus_tag="OO199_000989"
     CDS             32896..33747
                     /gene="tsf"
                     /locus_tag="OO199_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5535120.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33894..34619
                     /gene="pyrH"
                     /locus_tag="OO199_000990"
     CDS             33894..34619
                     /gene="pyrH"
                     /locus_tag="OO199_000990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5535121.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34911..35468
                     /gene="frr"
                     /locus_tag="OO199_000991"
     CDS             34911..35468
                     /gene="frr"
                     /locus_tag="OO199_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5535122.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35560..36756
                     /gene="ispC"
                     /locus_tag="OO199_000992"
     CDS             35560..36756
                     /gene="ispC"
                     /locus_tag="OO199_000992"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5535123.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36945..37703
                     /gene="ispU"
                     /locus_tag="OO199_000993"
                     /gene_synonym="uppS"
     CDS             36945..37703
                     /gene="ispU"
                     /locus_tag="OO199_000993"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5535124.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37716..38573
                     /gene="cdsA"
                     /locus_tag="OO199_000994"
     CDS             37716..38573
                     /gene="cdsA"
                     /locus_tag="OO199_000994"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5535125.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38585..39937
                     /gene="rseP"
                     /locus_tag="OO199_000995"
     CDS             38585..39937
                     /gene="rseP"
                     /locus_tag="OO199_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5535126.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39967..42399
                     /gene="bamA"
                     /locus_tag="OO199_000996"
     CDS             39967..42399
                     /gene="bamA"
                     /locus_tag="OO199_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5535127.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42521..43006
                     /gene="skp"
                     /locus_tag="OO199_000997"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42521..43006
                     /gene="skp"
                     /locus_tag="OO199_000997"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5535128.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43010..44035
                     /gene="lpxD"
                     /locus_tag="OO199_000998"
     CDS             43010..44035
                     /gene="lpxD"
                     /locus_tag="OO199_000998"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5535129.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44140..44595
                     /gene="fabZ"
                     /locus_tag="OO199_000999"
     CDS             44140..44595
                     /gene="fabZ"
                     /locus_tag="OO199_000999"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5535130.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44599..45387
                     /gene="lpxA"
                     /locus_tag="OO199_001000"
     CDS             44599..45387
                     /gene="lpxA"
                     /locus_tag="OO199_001000"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5535131.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45387..46535
                     /gene="lpxB"
                     /locus_tag="OO199_001001"
     CDS             45387..46535
                     /gene="lpxB"
                     /locus_tag="OO199_001001"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5535132.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46532..47128
                     /gene="rnhB"
                     /locus_tag="OO199_001002"
     CDS             46532..47128
                     /gene="rnhB"
                     /locus_tag="OO199_001002"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5535133.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47165..50647
                     /gene="dnaE"
                     /locus_tag="OO199_001003"
     CDS             47165..50647
                     /gene="dnaE"
                     /locus_tag="OO199_001003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5535134.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50660..51619
                     /gene="accA"
                     /locus_tag="OO199_001004"
     CDS             50660..51619
                     /gene="accA"
                     /locus_tag="OO199_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5535135.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51718..53859
                     /gene="ldcC"
                     /locus_tag="OO199_001005"
     CDS             51718..53859
                     /gene="ldcC"
                     /locus_tag="OO199_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5535136.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
BASE COUNT        12657 a        13268 c        15001 g        12969 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      661 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcataccacc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1681 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc aggcactttc cagccaaaaa
     2341 gctggtacag cgggatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2521 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaaaataa
     3841 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     4021 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4381 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcagg tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggctgaat acccgcctgg aagtggttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5341 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5461 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5581 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 ggtgctctgc ccgctgtatg gcgtgttgtc actgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5881 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     6001 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacagcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6661 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6781 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6961 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7261 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7321 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg gacgacccgg caaacacggc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag
     7501 ggagagggga ccgatcgagc gcaaattttg agagatatct tcttctgtct tgtaacagaa
     7561 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7621 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7681 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7741 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
     7921 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
     7981 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct
     8341 ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
     8461 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg
     8821 tggggtgatc tctcctcagc cagcctttat gcagctggtg cgtcaggagt tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtc ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgcg gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc
     9421 ggttccggca atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggt aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatacc tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    10081 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    10141 acatctaagc gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta
    10201 agatattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
    10261 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    10321 tcgtttacgt tattattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
    10381 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    10441 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    10501 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc
    10561 taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac
    10621 cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc
    10681 gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccacctga tcattcgcgg
    10741 ttttgcggca gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa
    10801 cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg
    10861 cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa
    10921 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt
    10981 ccagactggt tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct
    11041 gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc
    11101 tattgcaccg gtgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta
    11161 cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga
    11221 gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac
    11281 ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
    11341 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg
    11401 ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc
    11461 agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa
    11521 cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct
    11581 gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga
    11641 cgactctgtg ccactgctca atctgtacaa tccggtgaat cccgatttcg acttcaatgc
    11701 caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
    11761 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga
    11821 ctgggcagat caagaatctc ttaaccgcgt tgctgggacg accgataaac gtgatgacaa
    11881 acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt
    11941 cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc
    12001 accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat
    12061 tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga
    12121 gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
    12181 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga
    12241 atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc
    12301 ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtctga cgctgggcac
    12361 cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag
    12421 ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc
    12481 caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa
    12541 cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt
    12601 ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag
    12661 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc
    12721 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg
    12781 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct
    12841 ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12901 tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12961 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg
    13021 gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat
    13081 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg
    13141 gcgcaggata gccgttgtct gttgctcgac gaaccgactt cagcgctgga tatcgcccac
    13201 caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt
    13261 gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc
    13321 agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13381 atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt
    13441 tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg
    13501 cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcggctatt gaccccaatc
    13561 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13621 acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact
    13681 cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac
    13741 cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg
    13801 cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat
    13861 cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc
    13921 aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13981 tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt
    14041 tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    14101 ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct
    14161 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    14221 ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg
    14281 caacgctttc ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt
    14341 gagtaaacga attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac
    14401 ggcgctcacc tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc
    14461 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14521 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14581 agtactgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14641 ggggattacc gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc
    14701 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14761 gtcgccggta acgctgattt tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa
    14821 tcagttactg gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg
    14881 aacgctgacg caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg
    14941 tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt
    15001 ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat
    15061 tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc
    15121 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc
    15181 gttgattggt gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt
    15241 gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15301 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc
    15361 gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc
    15421 tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15481 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15541 cgtcatgctg gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag
    15601 cccggaagtg ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct
    15661 ggtaccgggt aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgac
    15721 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15781 ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg
    15841 tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga
    15901 tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg
    15961 ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc
    16021 gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac
    16081 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    16141 gggctttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct
    16201 ggtgttcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct
    16261 gctgtcgacc tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta
    16321 atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt
    16381 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcccgct
    16441 tcaaacgctg acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta
    16501 aaacgctcga cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16561 aagaagatac cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct
    16621 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg
    16681 cccggctgcg cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg
    16741 gaaagcgtac ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga
    16801 cgaccaccga atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa
    16861 tctggcacta cggcgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16921 acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg
    16981 aactctggca gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag
    17041 gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta
    17101 caggttaagg tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac
    17161 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    17221 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc
    17281 gtcgcctcgg tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17341 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17401 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17461 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17521 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17581 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17641 ggttcctgat tcgtagaaaa agtgaatggc tgctattcca tgttattcat aacaagttaa
    17701 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17761 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17821 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17881 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17941 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg
    18001 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    18061 gctggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    18121 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc
    18181 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt
    18241 attgccggtg aaattctttg gcgggctggg gacactcggc ggaggcatgg ggctagggcg
    18301 cgaaggacca accgtgcaga tcggcggcaa tatcagtcgc atggtgcttg atattttccg
    18361 cctgaaaggt gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc
    18421 tgcggccttt aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18481 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18541 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc
    18601 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18661 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18721 tattaccaaa tgggtactga tggggggtgc gattggcggt ctgtgtggat tgctggggtt
    18781 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18841 tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg
    18901 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18961 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg
    19021 gacgtttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    19081 ggggatcatt ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat
    19141 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc
    19201 gattcttgcg cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    19261 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19321 gggttagaat ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19381 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19441 agataacccg aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca
    19501 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19561 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19621 caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19681 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19741 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19801 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19861 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19921 gcagttgcgt agatttctgt acgtaaaatc atggggattt cgcggaccag tacatcacga
    19981 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    20041 tccgcattaa aggctttatt cacgccaatg ccgacaaaca ccgccagacc aacggcgtcc
    20101 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    20161 atgctggtga ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    20221 tgatccagcg ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc
    20281 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccgta gatggcaaat
    20341 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20401 tgaaaaggtc aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag
    20461 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20521 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20581 aatagcctgt ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20641 gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggttct gaatcgactc
    20701 ttttccactg gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20761 atcagcatat tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc
    20821 ggccttgtct ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc
    20881 aatgcttgtc gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg
    20941 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg
    21001 ttccagattc atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata
    21061 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    21121 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga
    21181 cagggcgacc agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact
    21241 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21301 actgttgatc ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21361 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21421 ggcggatttt cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc
    21481 cacgtacagc gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21541 cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc gtattcataa ccaaatgccg
    21601 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg
    21661 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21721 acagcagagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21781 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21841 ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga
    21901 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21961 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    22021 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg
    22081 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    22141 aagacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    22201 atggaagtcc agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag
    22261 cttaaattgc tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22321 gagatgtcat gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca
    22381 gcgataaatg actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg
    22441 actgacgatc gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac
    22501 gagctgcggc gcaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22561 cgtctggtgc atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta
    22621 aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg
    22681 aaaaagccgg gttattatct ttctgaagaa gcctatattg cccgcttgcg taaagaactt
    22741 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22801 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22861 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg
    22921 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22981 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    23041 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    23101 gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg
    23161 tttagccatc cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    23221 ttagagattt atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa
    23281 aaagaacggg tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc
    23341 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23401 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23461 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23521 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata
    23581 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23641 aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23701 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23761 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23821 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23881 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23941 aatggcggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    24001 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    24061 cagctgagcg atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    24121 atcgcgctga tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct
    24181 gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag
    24241 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24301 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt
    24361 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24421 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24481 gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa
    24541 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24601 aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24661 aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24721 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24781 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24841 atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg
    24901 gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg
    24961 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    25021 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    25081 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    25141 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    25201 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    25261 ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25321 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact
    25381 ttcacaggga cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg
    25441 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25501 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25561 gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact
    25621 ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg
    25681 ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag
    25741 cttggcgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc
    25801 gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg
    25861 ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt
    25921 acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc
    25981 ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa
    26041 cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc
    26101 gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa
    26161 gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg
    26221 caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt
    26281 aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg
    26341 tatgtggcgt tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcagcgct
    26401 ggctcattcc cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg
    26461 attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga
    26521 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26581 cacgctgaca gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct
    26641 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat
    26701 atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26761 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26821 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26881 cttattttag catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26941 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt
    27001 ccatgggatg gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa
    27061 gatgaaaaag tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca
    27121 gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc
    27181 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca
    27241 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27301 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27361 ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact
    27421 tcagagcgtg cgccaatgaa gcagttatct tcaataatgg tcgggttagc ctgcagcggc
    27481 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    27541 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    27601 ttaacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    27661 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    27721 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    27781 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    27841 attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct
    27901 tcgcgggtaa cggtgtctgc attggctggc gtgatctcgg cacggcgttc aaaagcggtt
    27961 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    28021 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28081 ttattaagcg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28141 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28201 cgcgccagca gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta
    28261 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28321 cgacggggct gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga
    28381 ataacctcat ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28441 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28501 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    28561 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    28621 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    28681 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    28741 agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt
    28801 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    28861 ttccagctat tccacagtgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc
    28921 agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg
    28981 tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag
    29041 gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc
    29101 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29161 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29221 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29281 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29341 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagtcc gcgccgcacc
    29401 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc
    29461 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg
    29521 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    29581 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    29641 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    29701 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    29761 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    29821 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc
    29881 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    29941 ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    30001 tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg
    30061 ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg
    30121 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30181 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30241 aacatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30301 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30361 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca
    30421 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30481 tggtctatga actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag
    30541 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat
    30601 tcatcacggg gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg
    30661 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    30721 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    30781 ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg
    30841 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    30901 gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt
    30961 cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    31021 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31081 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc
    31141 caggtacagg ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc
    31201 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31261 gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac
    31321 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31381 cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31441 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31501 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    31561 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    31621 ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt
    31681 cgacgtctga tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta
    31741 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    31801 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    31861 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    31921 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    31981 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    32041 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg
    32101 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32161 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32221 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32281 cggtactttc gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32341 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32401 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt
    32461 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    32521 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    32581 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    32641 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    32701 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    32761 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    32821 gcatatggcc ccctttttca cttttatatc tgtgtggttt aatgccgggc agatcacatc
    32881 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32941 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    33001 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33061 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33121 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33181 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33241 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33301 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33361 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33421 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33481 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33541 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33601 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33661 ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33721 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    33781 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33841 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33901 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33961 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    34021 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34081 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34141 cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34201 gtctgatgtc cgctattcca ttgaatggcg tgtgcgatag ctacagctgg gcagaagcta
    34261 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34321 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34381 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagattca accgcaacca
    34441 tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34501 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    34561 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34621 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg
    34681 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    34741 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    34801 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34861 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34921 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34981 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    35041 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35101 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35161 cctccgatct cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35221 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35281 cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35341 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35401 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35461 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35521 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35581 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35641 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35701 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35761 atgctacagc aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35821 atggcagcgc ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg
    35881 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35941 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    36001 ccggtcgata gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat
    36061 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36121 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc
    36181 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36241 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36301 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36361 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36421 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36481 gccgcaccgg attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36541 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36601 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36661 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa
    36721 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36781 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    36841 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36901 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36961 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    37021 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37081 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37141 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37201 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37261 tgcgtattat tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat
    37321 ctgaagcgct aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37381 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37441 tgcaaccaga tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg
    37501 cccctgtaga tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt
    37561 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37621 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37681 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37741 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37801 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37861 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37921 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37981 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    38041 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38101 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38161 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38221 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38281 cctggcaagg ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38341 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38401 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38461 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38521 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38581 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38641 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38701 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38761 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38821 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    38881 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38941 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    39001 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39061 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39121 cattacagta gcgccatttg gcagcgacca acggcgggat gtaaagctcg atttacgtca
    39181 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39241 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39301 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39361 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39421 ggggagtccc ttgtctttga cattaatccc ggggagtaaa ccgggtaatg gtaaagcgat
    39481 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39541 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39601 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39661 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39721 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39781 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    39841 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39901 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39961 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    40021 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40081 gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat
    40141 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40201 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40261 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40321 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40381 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40441 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40501 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40561 ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa
    40621 ctggcgggcg atcttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40681 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40741 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40801 gggcactccg ctgaaattga gcagctgact aagatcgagc cgggcgagct gtataacggc
    40861 actaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40921 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40981 gtgaacgttg atgtgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    41041 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg
    41101 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41161 gataccgata cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg
    41221 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41281 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41341 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41401 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41461 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41521 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41581 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    41641 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41701 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41761 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41821 gtgccgatcg atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt
    41881 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41941 accgtgcgtg gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag
    42001 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42061 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42121 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc
    42181 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42241 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42301 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42361 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42421 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42481 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    42541 tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    42601 caatatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    42661 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    42721 aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt
    42781 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    42841 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    42901 tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag
    42961 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    43021 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43081 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43141 cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga
    43201 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43261 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43321 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43381 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43441 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43501 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    43561 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    43621 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt
    43681 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    43741 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    43801 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    43861 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    43921 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    43981 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    44041 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44101 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44161 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44221 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44281 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44341 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44401 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44461 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    44521 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    44581 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    44641 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    44701 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    44761 aaaattggcc gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat
    44821 ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    44881 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    44941 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    45001 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45061 ggcatgaccg cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc
    45121 tccggtgtgg cgcaggacgt ccctccttat gtcattgcgc agggtaacca cgcaacgccg
    45181 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45241 atccgcaatg cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45301 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45361 cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg
    45421 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45481 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    45541 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    45601 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    45661 atgtttttgt tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa
    45721 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    45781 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    45841 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    45901 tgccgttaga tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc
    45961 actgtctggc attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt
    46021 tcttgaaaac tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc
    46081 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46141 tgtcggttca tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac
    46201 tactggcgtc ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46261 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46321 tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt
    46381 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46441 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46501 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    46561 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    46621 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    46681 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    46741 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    46801 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    46861 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc
    46921 agaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct
    46981 ggatattgtt ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca
    47041 tctggaaaaa ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt
    47101 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47161 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47221 tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact
    47281 ggcgatcacc gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg
    47341 cgcagggatt aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga
    47401 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt
    47461 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    47521 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt
    47581 cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga
    47641 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    47701 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    47761 taacgatgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    47821 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    47881 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa
    47941 caccgttgag atcgccaaac gctgtaacgt aactatgcgt cttggtgaat acttcctgcc
    48001 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg
    48061 cctggaagag cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc
    48121 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg
    48181 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48241 gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48301 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48361 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc
    48421 ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa
    48481 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat
    48541 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    48601 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    48661 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    48721 gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac
    48781 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    48841 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    48901 tggcgagcca ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat
    48961 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    49021 gatcaagcgt ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg
    49081 ccctggtccg ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49141 agagatctcc tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc
    49201 aacctacggc attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg
    49261 ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga
    49321 gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga
    49381 actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc
    49441 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc
    49501 ggcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    49561 cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    49621 tctttaccat ttccacgtca acgacgacgg cgaaatcgtg tatggtattg gcgccatcaa
    49681 aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    49741 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct
    49801 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    49861 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    49921 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    49981 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    50041 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50101 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct
    50161 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50221 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50281 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50341 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50401 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50461 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    50521 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    50581 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    50641 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    50701 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    50761 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    50821 cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta
    50881 taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc
    50941 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat
    51001 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51061 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51121 gatgcctatc atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga
    51181 gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc
    51241 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg
    51301 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51361 gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat
    51421 cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg
    51481 cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    51541 ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    51601 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    51661 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    51721 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    51781 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    51841 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    51901 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    51961 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    52021 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52081 aacgaatatc ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52141 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52201 ccggttggct gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct
    52261 atttcggtca ccgagcttgg ttcgttgctt gaccacaccg ggccacatct ggaagcggaa
    52321 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaacatcg
    52381 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52441 cgcaactgcc ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52501 ctgaaaccga cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact
    52561 cgcgacagca tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg
    52621 gtgatcacca actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg
    52681 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca
    52741 atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa
    52801 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa
    52861 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    52921 agttatccca ttgttgcttc ggttgagacg gcggcggcga tgctgcgtgg taatccgggc
    52981 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    53041 ctgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53101 gccgaatgca ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc
    53161 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53221 ggcaatatga gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt
    53281 gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag tattggcatc
    53341 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat
    53401 ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac
    53461 cacaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    53521 ctttccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    53581 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc
    53641 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    53701 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    53761 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    53821 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    53881 cgagtaacgt gctgt
//
LOCUS       DAJTNV010000017        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNV010000017 DAJTNV010000000
VERSION     DAJTNV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(11..322)
                     /locus_tag="OO199_001006"
     CDS             complement(11..322)
                     /locus_tag="OO199_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5535137.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO199_001007"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO199_001007"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO199_001008"
     CDS             701..1054
                     /locus_tag="OO199_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5535138.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO199_001009"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO199_001009"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5535139.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO199_001010"
     CDS             complement(2810..3280)
                     /locus_tag="OO199_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5535140.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO199_001011"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO199_001011"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5535141.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO199_001012"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO199_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5535142.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO199_001013"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO199_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5535143.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO199_001014"
     CDS             complement(5283..8432)
                     /locus_tag="OO199_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5535144.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO199_001015"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO199_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5535145.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO199_001016"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO199_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5535146.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO199_001017"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO199_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5535147.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO199_001018"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO199_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5535148.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO199_001019"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO199_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5535149.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO199_001020"
     CDS             14911..15288
                     /locus_tag="OO199_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5535150.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO199_001021"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO199_001021"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5535151.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO199_001022"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO199_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5535152.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO199_001022"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO199_001023"
     CDS             18099..18428
                     /locus_tag="OO199_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5535153.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO199_001024"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO199_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5535154.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO199_001025"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO199_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5535155.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO199_001026"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO199_001026"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5535156.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO199_001027"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO199_001027"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5535157.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO199_001028"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO199_001028"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5535158.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO199_001029"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO199_001029"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5535159.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO199_001030"
     CDS             complement(25481..27157)
                     /locus_tag="OO199_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5535160.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO199_001031"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO199_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5535161.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO199_001032"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO199_001032"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5535162.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO199_001033"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO199_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5535163.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO199_001034"
     CDS             complement(31205..31999)
                     /locus_tag="OO199_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5535164.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO199_001035"
     CDS             32137..32478
                     /locus_tag="OO199_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5535165.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO199_001036"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO199_001036"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5535166.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO199_001037"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO199_001037"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5535167.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO199_001038"
     CDS             36394..37686
                     /locus_tag="OO199_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5535168.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="OO199_001039"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="OO199_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5535169.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="OO199_001040"
     CDS             complement(38219..38677)
                     /locus_tag="OO199_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5535170.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO199_001041"
     CDS             complement(38674..39591)
                     /locus_tag="OO199_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5535171.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO199_001042"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO199_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5535172.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO199_001043"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO199_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5535173.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO199_001044"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO199_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5535174.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO199_001045"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO199_001045"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5535175.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO199_001046"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO199_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO199_001047"
     CDS             43550..44236
                     /locus_tag="OO199_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5535176.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO199_001048"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO199_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO199_001049"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO199_001049"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5535177.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO199_001050"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO199_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5535178.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OO199_001051"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OO199_001051"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5535179.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OO199_001052"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OO199_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5535180.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OO199_001053"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OO199_001053"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5535181.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53211
                     /locus_tag="OO199_001054"
     CDS             52938..>53211
                     /locus_tag="OO199_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5535182.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13691 c        14020 g        12670 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cagtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt c
//
LOCUS       DAJTNV010000018        52226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNV010000018 DAJTNV010000000
VERSION     DAJTNV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OO199_001055"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OO199_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5535183.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="OO199_001056"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="OO199_001056"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5535184.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="OO199_001057"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="OO199_001057"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5535185.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="OO199_001058"
     CDS             complement(5906..6949)
                     /locus_tag="OO199_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5535186.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OO199_001059"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OO199_001059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5535187.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OO199_001060"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OO199_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5535188.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="OO199_001061"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="OO199_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="OO199_001062"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="OO199_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5535189.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OO199_001063"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OO199_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5535190.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="OO199_001064"
     CDS             15474..15830
                     /locus_tag="OO199_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5535191.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="OO199_001065"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="OO199_001065"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5535192.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="OO199_001066"
     CDS             16989..17189
                     /locus_tag="OO199_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5535193.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OO199_001067"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OO199_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5535194.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OO199_001068"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OO199_001068"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5535195.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="OO199_001069"
     CDS             complement(20101..20964)
                     /locus_tag="OO199_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5535196.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OO199_001070"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OO199_001070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5535197.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OO199_001071"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OO199_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5535198.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OO199_001072"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OO199_001072"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5535199.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="OO199_001073"
     CDS             24133..24705
                     /locus_tag="OO199_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5535200.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="OO199_001074"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="OO199_001074"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5535201.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="OO199_001075"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="OO199_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5535202.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="OO199_001076"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="OO199_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="OO199_001077"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="OO199_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5535203.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="OO199_001078"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="OO199_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5535204.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="OO199_001079"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="OO199_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5535205.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="OO199_001080"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="OO199_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5535206.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="OO199_001081"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="OO199_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="OO199_001082"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="OO199_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5535207.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="OO199_001083"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="OO199_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5535208.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="OO199_001084"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="OO199_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5535209.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="OO199_001085"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="OO199_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5535210.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="OO199_001086"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="OO199_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="OO199_001087"
     CDS             complement(39099..40160)
                     /locus_tag="OO199_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5535211.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="OO199_001088"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="OO199_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5535212.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="OO199_001089"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="OO199_001089"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5535213.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OO199_001090"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OO199_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5535214.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OO199_001091"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OO199_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5535215.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OO199_001092"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OO199_001092"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5535216.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="OO199_001093"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="OO199_001093"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5535217.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="OO199_001094"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="OO199_001094"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5535218.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="OO199_001095"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="OO199_001095"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5535219.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="OO199_001096"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="OO199_001096"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5535220.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..52105)
                     /locus_tag="OO199_001097"
     CDS             complement(50969..52105)
                     /locus_tag="OO199_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5535221.1"
                     /translation="MSVKLRLPQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTES
                     HQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIV
                     TNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILL
                     RMKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSP
                     TSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAI
                     DDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAE
                     YVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            52144..>52226
                     /locus_tag="OO199_001098"
     CDS             52144..>52226
                     /locus_tag="OO199_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5535222.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSI"
BASE COUNT        12209 a        13768 c        13554 g        12695 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgtatt
    52081 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
    52141 ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg
    52201 gttcttgaaa atcaactttc gatcag
//
LOCUS       DAJTNV010000019        49530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNV010000019 DAJTNV010000000
VERSION     DAJTNV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO199_001099"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO199_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5535223.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO199_001100"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO199_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5535224.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO199_001101"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO199_001101"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5535225.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO199_001102"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO199_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5535226.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO199_001103"
     CDS             5718..6659
                     /locus_tag="OO199_001103"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5535227.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO199_001104"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO199_001104"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5535228.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO199_001105"
     CDS             7679..8929
                     /locus_tag="OO199_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5535229.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO199_001106"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO199_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO199_001107"
                     /pseudo
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO199_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10866..12116
                     /locus_tag="OO199_001108"
     CDS             10866..12116
                     /locus_tag="OO199_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5535230.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO199_001109"
     CDS             complement(12172..13260)
                     /locus_tag="OO199_001109"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5535231.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO199_001110"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO199_001110"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5535232.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO199_001111"
     CDS             complement(14194..15243)
                     /locus_tag="OO199_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5535233.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO199_001112"
     CDS             15342..16223
                     /locus_tag="OO199_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535234.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO199_001113"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO199_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5535235.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO199_001114"
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO199_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR5535236.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO199_001115"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO199_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO199_001116"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO199_001116"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5535237.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO199_001117"
     CDS             21991..23067
                     /locus_tag="OO199_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5535238.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO199_001118"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO199_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5535239.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO199_001119"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO199_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5535240.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO199_001120"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO199_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5535241.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO199_001121"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO199_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5535242.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO199_001122"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO199_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO199_001123"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO199_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5535243.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO199_001124"
     CDS             complement(30809..31048)
                     /locus_tag="OO199_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5535244.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO199_001125"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO199_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5535245.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO199_001126"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO199_001126"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5535246.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OO199_001127"
     CDS             complement(32934..33629)
                     /locus_tag="OO199_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5535247.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OO199_001128"
     CDS             complement(33626..34024)
                     /locus_tag="OO199_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5535248.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OO199_001129"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OO199_001129"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5535249.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO199_001130"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO199_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5535250.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO199_001131"
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO199_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5535251.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37377..38138
                     /locus_tag="OO199_001132"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OO199_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OO199_001133"
     CDS             complement(38255..38833)
                     /locus_tag="OO199_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5535252.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OO199_001134"
     CDS             39003..39590
                     /locus_tag="OO199_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5535253.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OO199_001135"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OO199_001135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5535254.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO199_001136"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO199_001136"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5535255.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="OO199_001137"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="OO199_001137"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5535256.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="OO199_001138"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="OO199_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5535257.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="OO199_001139"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="OO199_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5535258.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="OO199_001140"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="OO199_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5535259.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="OO199_001141"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="OO199_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5535260.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="OO199_001142"
     CDS             complement(48911..49018)
                     /locus_tag="OO199_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5535261.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49530)
                     /locus_tag="OO199_001143"
                     /pseudo
     CDS             complement(49078..49530)
                     /locus_tag="OO199_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11954 a        12201 c        13310 g        12065 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ctagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgta gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gcggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagatc cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cacagaatgt ctgaattaaa
     6661 aatctcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgaccg gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgctaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcagata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtatg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagcag gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagc ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaatatt tggattgata
    18001 attgttatcg tttgcattat cgttacgaca caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agactctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcgggtct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gattgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atctgcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggatg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctcaggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc gactgcgacg
    34921 cagtgatgat tggtcgcggg gcgttcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagtcctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat tcagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagagc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattata cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatttgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatacacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatctttca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccggtgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttactggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc
//
LOCUS       DAJTNV010000020        48595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNV010000020 DAJTNV010000000
VERSION     DAJTNV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            17..1267
                     /locus_tag="OO199_001144"
     CDS             17..1267
                     /locus_tag="OO199_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5535262.1"
                     /translation="MSKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIAFSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1264..2229
                     /gene="yggF"
                     /locus_tag="OO199_001145"
     CDS             1264..2229
                     /gene="yggF"
                     /locus_tag="OO199_001145"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5535263.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2251..2760
                     /gene="fumE"
                     /locus_tag="OO199_001146"
     CDS             2251..2760
                     /gene="fumE"
                     /locus_tag="OO199_001146"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5535264.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2757..3470
                     /locus_tag="OO199_001147"
     CDS             2757..3470
                     /locus_tag="OO199_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5535265.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3442..4124)
                     /locus_tag="OO199_001148"
                     /pseudo
     CDS             complement(3442..4124)
                     /locus_tag="OO199_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4118..4795)
                     /locus_tag="OO199_001149"
     CDS             complement(4118..4795)
                     /locus_tag="OO199_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5535266.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4783..5166)
                     /locus_tag="OO199_001150"
     CDS             complement(4783..5166)
                     /locus_tag="OO199_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5535267.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5182..5490)
                     /locus_tag="OO199_001151"
     CDS             complement(5182..5490)
                     /locus_tag="OO199_001151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535268.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5491..6069)
                     /locus_tag="OO199_001152"
     CDS             complement(5491..6069)
                     /locus_tag="OO199_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5535269.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6092..6523)
                     /locus_tag="OO199_001153"
                     /pseudo
     CDS             complement(6092..6523)
                     /locus_tag="OO199_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6895..7914
                     /gene="epd"
                     /locus_tag="OO199_001154"
     CDS             6895..7914
                     /gene="epd"
                     /locus_tag="OO199_001154"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5535270.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7964..9127
                     /gene="pgk"
                     /locus_tag="OO199_001155"
     CDS             7964..9127
                     /gene="pgk"
                     /locus_tag="OO199_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5535271.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9342..10421
                     /gene="fbaA"
                     /locus_tag="OO199_001156"
     CDS             9342..10421
                     /gene="fbaA"
                     /locus_tag="OO199_001156"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5535272.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10612..11472
                     /gene="mscS"
                     /locus_tag="OO199_001157"
     CDS             10612..11472
                     /gene="mscS"
                     /locus_tag="OO199_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5535273.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11611..12246
                     /gene="argO"
                     /locus_tag="OO199_001158"
     CDS             11611..12246
                     /gene="argO"
                     /locus_tag="OO199_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5535274.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12339..13079
                     /locus_tag="OO199_001159"
     CDS             12339..13079
                     /locus_tag="OO199_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5535275.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13246..14196
                     /locus_tag="OO199_001160"
     CDS             13246..14196
                     /locus_tag="OO199_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535276.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14138..15616)
                     /gene="scpC"
                     /locus_tag="OO199_001161"
     CDS             complement(14138..15616)
                     /gene="scpC"
                     /locus_tag="OO199_001161"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5535277.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15640..16416)
                     /gene="scpB"
                     /locus_tag="OO199_001162"
     CDS             complement(15640..16416)
                     /gene="scpB"
                     /locus_tag="OO199_001162"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5535278.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16427..17422)
                     /gene="meaB"
                     /locus_tag="OO199_001163"
     CDS             complement(16427..17422)
                     /gene="meaB"
                     /locus_tag="OO199_001163"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5535279.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17415..19559)
                     /gene="scpA"
                     /locus_tag="OO199_001164"
     CDS             complement(17415..19559)
                     /gene="scpA"
                     /locus_tag="OO199_001164"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5535280.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19763..20656)
                     /gene="argP"
                     /locus_tag="OO199_001165"
     CDS             complement(19763..20656)
                     /gene="argP"
                     /locus_tag="OO199_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5535281.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21039..21698
                     /gene="rpiA"
                     /locus_tag="OO199_001166"
     CDS             21039..21698
                     /gene="rpiA"
                     /locus_tag="OO199_001166"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5535282.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21954..23186
                     /gene="serA"
                     /locus_tag="OO199_001167"
     CDS             21954..23186
                     /gene="serA"
                     /locus_tag="OO199_001167"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5535283.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23415..23474
                     /gene="ibsC"
                     /locus_tag="OO199_001168"
     CDS             23415..23474
                     /gene="ibsC"
                     /locus_tag="OO199_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5535284.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23807..23866
                     /locus_tag="OO199_001169"
     CDS             23807..23866
                     /locus_tag="OO199_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5535285.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23967..24569)
                     /locus_tag="OO199_001170"
     CDS             complement(23967..24569)
                     /locus_tag="OO199_001170"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5535286.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24590..24773)
                     /gene="ssrS"
                     /locus_tag="OO199_001171"
     ncRNA           complement(24590..24773)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO199_001171"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24815..25144)
                     /gene="zapA"
                     /locus_tag="OO199_001172"
     CDS             complement(24815..25144)
                     /gene="zapA"
                     /locus_tag="OO199_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5535287.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25312..25890
                     /gene="ygfB"
                     /locus_tag="OO199_001173"
     CDS             25312..25890
                     /gene="ygfB"
                     /locus_tag="OO199_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5535288.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25916..27241
                     /gene="pepP"
                     /locus_tag="OO199_001174"
     CDS             25916..27241
                     /gene="pepP"
                     /locus_tag="OO199_001174"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5535289.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27238..28416
                     /gene="ubiH"
                     /locus_tag="OO199_001175"
                     /gene_synonym="visB"
     CDS             27238..28416
                     /gene="ubiH"
                     /locus_tag="OO199_001175"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5535290.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28439..29641
                     /gene="ubiI"
                     /locus_tag="OO199_001176"
                     /gene_synonym="visC"
     CDS             28439..29641
                     /gene="ubiI"
                     /locus_tag="OO199_001176"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5535291.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29715..29798)
                     /locus_tag="OO199_001177"
     CDS             complement(29715..29798)
                     /locus_tag="OO199_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535292.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30089..31183
                     /gene="gcvT"
                     /locus_tag="OO199_001178"
     CDS             30089..31183
                     /gene="gcvT"
                     /locus_tag="OO199_001178"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5535293.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31207..31596
                     /gene="gcvH"
                     /locus_tag="OO199_001179"
     CDS             31207..31596
                     /gene="gcvH"
                     /locus_tag="OO199_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5535294.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31715..34588
                     /gene="gcvP"
                     /locus_tag="OO199_001180"
     CDS             31715..34588
                     /gene="gcvP"
                     /locus_tag="OO199_001180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5535295.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34855..35597
                     /locus_tag="OO199_001181"
                     /pseudo
     CDS             34855..35597
                     /locus_tag="OO199_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35654..37087)
                     /gene="bglA"
                     /locus_tag="OO199_001182"
     CDS             complement(35654..37087)
                     /gene="bglA"
                     /locus_tag="OO199_001182"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5535296.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37132..37442
                     /locus_tag="OO199_001183"
                     /pseudo
     CDS             37132..37442
                     /locus_tag="OO199_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37606..38265
                     /locus_tag="OO199_001184"
     CDS             37606..38265
                     /locus_tag="OO199_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5535297.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38461..39441)
                     /gene="ygfZ"
                     /locus_tag="OO199_001185"
     CDS             complement(38461..39441)
                     /gene="ygfZ"
                     /locus_tag="OO199_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5535298.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39684..39950
                     /gene="sdhE"
                     /locus_tag="OO199_001186"
     CDS             39684..39950
                     /gene="sdhE"
                     /locus_tag="OO199_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5535299.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39931..40338
                     /locus_tag="OO199_001187"
     CDS             39931..40338
                     /locus_tag="OO199_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5535300.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40378..40899)
                     /gene="fldB"
                     /locus_tag="OO199_001188"
     CDS             complement(40378..40899)
                     /gene="fldB"
                     /locus_tag="OO199_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5535301.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41011..41907
                     /gene="xerD"
                     /locus_tag="OO199_001189"
     CDS             41011..41907
                     /gene="xerD"
                     /locus_tag="OO199_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5535302.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41932..42642
                     /gene="dsbC"
                     /locus_tag="OO199_001190"
     CDS             41932..42642
                     /gene="dsbC"
                     /locus_tag="OO199_001190"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5535303.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42648..44381
                     /gene="recJ"
                     /locus_tag="OO199_001191"
     CDS             42648..44381
                     /gene="recJ"
                     /locus_tag="OO199_001191"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5535304.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44472..45570
                     /gene="prfB"
                     /locus_tag="OO199_001192"
     CDS             join(44472..44546,44548..45570)
                     /gene="prfB"
                     /locus_tag="OO199_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5535305.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45580..47097
                     /gene="lysS"
                     /locus_tag="OO199_001193"
     CDS             45580..47097
                     /gene="lysS"
                     /locus_tag="OO199_001193"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5535306.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47140..47688)
                     /gene="idi"
                     /locus_tag="OO199_001194"
     CDS             complement(47140..47688)
                     /gene="idi"
                     /locus_tag="OO199_001194"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5535307.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47743..47814
                     /locus_tag="OO199_001195"
     CDS             47743..47814
                     /locus_tag="OO199_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535308.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47811..47936)
                     /locus_tag="OO199_001196"
     CDS             complement(47811..47936)
                     /locus_tag="OO199_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535309.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47938..>48595)
                     /gene="uacT"
                     /locus_tag="OO199_001197"
     CDS             complement(47938..>48595)
                     /gene="uacT"
                     /locus_tag="OO199_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5535310.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11662 a        12184 c        13117 g        11632 t
ORIGIN      
        1 ctgaacataa aacactatca gtaagttgga gtcattaccc gaatttgaac tgccagaaat
       61 taccgataat gaactgttag tgagtgtaat ttctgacagc gtctgtttat cgacctggaa
      121 agcggcgtta ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt
      181 cattaccggg catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa
      241 atataaaaaa ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc
      301 agcgggctac agctacgaat attttggcgg caatgccact tatatgatta ttcccgaaat
      361 cgccattaat ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct
      421 ggcagagcct atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata
      481 tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc
      541 gggtccgatg ggcattggcg ctatcgatta cgccattaac ggtggcatac aaccgtcacg
      601 ggtggtggtg gtcgatatcg acgacaaacg cctggcgcag gtgcagaagc tgctgccggt
      661 ggatctggcg gccagtaaag gcattgagct agtgtatgtg aataccaaag ggatgagcga
      721 tcctatccag atgctgcggg cgctgacagg agatgtcggg ttcgatgaca tttttgttta
      781 tgcggcggtg cctgctgtcg ttgagatggc tgacgaatta ctggcggaag atggctgtct
      841 gaacttcttt gccgggctga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt
      901 ccattacaac agcacgcacg tagtcggtac atccggcggt tcaacggacg acatgaaaga
      961 ggcgattgcc tttagcgcca caggacagtt acagccgtcg tttatggtga cccatattgg
     1021 tggcctggat gcggtgccag aaaccgtgct caatctgccg gatatccctg gcggtaaaaa
     1081 actcatttat aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg
     1141 caaaaccgat ccgctgttta aagagttggc gcggctggtt gaggaaacgc acggcatctg
     1201 gaatgaacag gccgagaaat atctgctggc acaatttggc gttgatatcg gggaggccgc
     1261 gcaatgatgt ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc
     1321 tggccgcaca ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg
     1381 cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt
     1441 gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc
     1501 gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg
     1561 ctggcggtca tggctttcgc tcctcgagat agcctgttac atgcgcccga tatgtatatg
     1621 aaaaagctgg ttgttaatcg actggctgcc ggggcgattg atctgtcttt gcctctggcg
     1681 gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt
     1741 acgctggata aaccgcgtct gagtgccgcg attgaagaag caacacagct gggcgtgaag
     1801 gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat
     1861 ccttacgatg tgatgtacac catcggcggt gcgccagaag gcgtgatttc tgcctgtgcc
     1921 gttaaagcgt taggcgggga tatgcaggcc gagctgattg acttttgtca ggctaaaggg
     1981 gattacacgg aaaatcggca gattgcagag caggagcgca agcgttgtaa ggcaatgggc
     2041 gttgacgtta accgtgtgta ctcgctcgat gaactggtga gggggaacga tatcctcttt
     2101 agcgctacgg gcgtgacggg cggcgagttg gtgaacggta tccaacagac ggcgaatggg
     2161 gtgcggacgc agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc
     2221 ctgcattgat ggtgatctat cggaagacca atggcgacgc tgacagaaga tgatgtgctt
     2281 gagcaactgg atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg
     2341 ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtc
     2401 gaatatgcag tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgcg
     2461 ctgcgtttga tttatgcgct ggggaaaatg gataaatggc tgtatgccga tatcacgcac
     2521 ttttcccagt actggcatta cctgaacgaa caggatgaaa cgcccggatt tgccgatgat
     2581 ataacctggg attttatcag caatgtcaac agtataactc gcaatgcgac gctctacgat
     2641 gcgttaaaag cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcgga
     2701 atggtcaaaa cggcgctgac gctggcagta acgaccacct taaaggaatt aacgccgtga
     2761 aaattgaatt aacggtgaat gggctgaacg tgcaagcgca gtaccatgat gaagagatag
     2821 aacgcgtgca taaactgttg ctgcgtatgc tggcggcctt acagacggta aatccgcagc
     2881 ggcgtacggt ggtttttctt tgcgctccgc cggggacggg taaatctacc ctaaccacct
     2941 tctgggaata tctcgcgcaa caagacccgg agctgcccgc tattcaggcg ctcccgatgg
     3001 atggttttca ccattacaat agctggctgg acgcgcatca attgcgcccc ttcaaaggcg
     3061 caccagagac atttgacgtt gcgaaactgg cggaaaatct gcgccaggtg gtggaagggg
     3121 attgtacgtg gccgcagtac gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg
     3181 ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt gctggacgac gagaagtggt
     3241 gccagcttgc gcaattttgt gatttctcga tctttatcaa cgcgccagct gctgcgttgc
     3301 gggagcgtct ggtggggcgc aagctggcgg gaggggtatc gctggcagat gcagaagcct
     3361 tttatgaccg taccgacggg ccgaatgttc gccgggtgct ggaagagagt ctgcctgcaa
     3421 atttgacctt aatgatgacc gctacgggtg aatatcgtct ggtggattaa cgttcctgat
     3481 tacaccatct tccagccgca ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg
     3541 actgattgct accacgctcg tgcctcgctg gcggcacttc tccagccagc tttcaaacac
     3601 gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc agcagtaata gtggcggaga
     3661 gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaacgg
     3721 gtgcgagttg gcagtgtctg ctaactgaca acattgcagg gcagcatggg tacgttgagt
     3781 gatttcatcc gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt
     3841 gctgtgaaaa agttggtttt ccgcttcctg aaacagaaca gtacgccaac ctttgccgcg
     3901 cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt accggctgtg
     3961 ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt tccctgcgcc attgtcaccg
     4021 gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt
     4081 gccgcgccag gccagcgata agatatctta tttaacgtta acatagtggc tggagcgctc
     4141 cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa
     4201 aaagaataat cagcgaacca cgttcaaacg cccagtgttg taagcgctgc aacagcattt
     4261 cgctggcctg cggcgtcagg cggctaaagg cttcatcaag aattaagagc tttggctgca
     4321 tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc accggagagt gttgccgggt
     4381 gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc cgcgccataa
     4441 tctctgcttc agcaagacac agattttctg gcccaaaggc aacttcttct tccacgctaa
     4501 aggtacagcc ggagagctgc aaatagggcg attgctggac gagctggatg gtggcggatt
     4561 gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg cctgttatct
     4621 ctcttggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta cttttgccac
     4681 tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag tcataacaga
     4741 acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta accatatcca
     4801 cgctccgaat tctgtcagca tcagcaagat catggtgtaa cgcgctacgc gttgtagggt
     4861 gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg cccgcatatc
     4921 cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagcg gaataattaa
     4981 cgcgggcata gcacgtaaac gctgatacca gccctcatcc agcggcacgc cgcgggcacg
     5041 ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca ataacggccc
     5101 ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc gaataaaaca
     5161 ttgcacgggg acgtactgca ttcacaactg tgaggttgaa acaatcgcca gaatcagcaa
     5221 ccagagcgta atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc ttagccattc
     5281 tggtagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca tcagccagac
     5341 gacatatttt gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa aggtcgccgc
     5401 gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg ccgccaatgc
     5461 ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg gcagccattg ccggaaaatg
     5521 gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg ctgtaagaat
     5581 tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca ggttttgccc
     5641 catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac cgccgaacag
     5701 cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca cgccgctgac
     5761 aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg agaccaggcc
     5821 gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca atccccagag
     5881 taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta ccgcgctaat
     5941 aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca gttgcccgcc
     6001 gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg aaaagtggct
     6061 acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata tgcaactcgt
     6121 cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg ctaccgatca
     6181 ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca agcatcgtgc
     6241 cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc gtgccgttta
     6301 gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca
     6361 aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta ggcggcatga agttgatgtt
     6421 gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg cgcagtgcgt
     6481 aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat cattggagta ctccgaaact
     6541 cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt
     6601 tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa
     6661 attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgcttt ctttgactgg
     6721 acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa
     6781 atgttcgaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt
     6841 tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc
     6901 gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat
     6961 gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg
     7021 ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg ggaagtacga
     7081 caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca
     7141 ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta
     7201 tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa agtgctcttt
     7261 tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa
     7321 cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc
     7381 gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac cacaattcac
     7441 tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg
     7501 gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgctggtat cacacgattt
     7561 tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg
     7621 acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg
     7681 ttgctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg
     7741 ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc
     7801 cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt
     7861 gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca
     7921 agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat
     7981 gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt
     8041 aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact
     8101 ggccctgaaa caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg
     8161 cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc
     8221 taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact
     8281 ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc
     8341 caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg
     8401 cgcgcaggct tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct
     8461 gctggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc gcccgatggt
     8521 ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact ccctgtctaa
     8581 aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg
     8641 ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta aacgtctgct
     8701 gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac
     8761 tgcaccggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga tcctggatat
     8821 cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg
     8881 gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc
     8941 taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc
     9001 aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt
     9061 cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa
     9121 gaagtaaaaa atcacagggc agggaaacct gcacttgttt cagcgcgctt ttagagcacg
     9181 cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca
     9241 ggagcttaca taagtaagtg actgggggga gcgaacgcag acgcagcaca tgcaacttga
     9301 agtattatga gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt
     9361 tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag
     9421 aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac
     9481 tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt
     9541 cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct atcctgggcg
     9601 cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc
     9661 tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg
     9721 gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg atcgacctgt
     9781 ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag cgtatgtcca
     9841 aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg
     9901 acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat
     9961 acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc ggtaacgtac
    10021 acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg
    10081 aatatgtttc taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg
    10141 gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta gtgaaaatga
    10201 acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga
    10261 acgaagctta tctgcagggt cagctgggta acccgaaagg cgaagatcag ccgaacaaga
    10321 aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg
    10381 aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agtcattcct ttctgcttat
    10441 ctcaaggccc gctctgcggg tctttttttc gccaaaagca aggtgattca gatgagaaat
    10501 ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact gcccgtcata
    10561 aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg aatggaagat
    10621 ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg
    10681 ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt tggtttgatt
    10741 atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc
    10801 actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt tacgctaatc
    10861 gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca
    10921 ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc tggcgtgtta
    10981 cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg cgtagccggt
    11041 actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg taaaattatc
    11101 gttattccga acggtaaaat tattgccgga aatattatta acttctcccg cgagccagtt
    11161 cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca ggttaagcag
    11221 atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga aatgactgtg
    11281 cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc
    11341 ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc
    11401 gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac
    11461 aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc ttattagtta
    11521 ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga tatagtctct
    11581 gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca aggtcttgct
    11641 cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat gaatcagggc
    11701 attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga tttggtcctg
    11761 atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg gttgctagcg
    11821 ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc ttttaaaaca
    11881 gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg cagatggaaa
    11941 attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct ggatactttt
    12001 gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc
    12061 gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc agcctggctg
    12121 gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt gggatgtgtc
    12181 atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca agccttgttc
    12241 agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct ccagaggtcc
    12301 tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa gttatcgccc
    12361 tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg ccggatggac
    12421 cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt gccactcttg
    12481 cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag gcagatgagc
    12541 gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct
    12601 cagcgaattt acgcacccag ccagattatg actatcagga tggtaaaagt atcctgaaag
    12661 gctaccgagc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa ctgaattcct
    12721 tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg ctgggcgtgg
    12781 cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac gcgattcatc
    12841 aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat agcgtgcgct
    12901 accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc
    12961 cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat caggtcgatg
    13021 tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca gcacaataat
    13081 acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc tcctttttct
    13141 gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt catcaaggat
    13201 aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga catttttatt
    13261 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    13321 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    13381 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggtcag
    13441 gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga taatgaaatc
    13501 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    13561 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac tgcccagcca
    13621 gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt agattttatt
    13681 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    13741 ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa
    13801 gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc taacttcgaa
    13861 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc
    13921 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    13981 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg
    14041 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatc
    14101 ttcagcatat tgctgaagat cgtgacggga caaaccatta acccagcatc gagccggttg
    14161 caattaaatt acggtgtaag tcgaagacgt ggctaagatc gtggtgaata tgtccgccgg
    14221 gcgcattttc cagatagcga tgcagataat cccgatacat aggatgtgca caattatcaa
    14281 tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc ccttgttcgg
    14341 tgatgatcac tttgacgctg tgttcgctgt gatcaacatg gctgcacatt ggcacgacgg
    14401 ttgagatctt gccttcttta gcaatcgacg gagccataaa gatcgacaga tacgcgttgc
    14461 gttcaaaatc accgctgccg ccgatgccgt tcatcagatc gaccccggct acgtgcgttg
    14521 aatttgcatg cccgtaaata tcaaactcca ggccgacgtt cagagcgatg acgcccagac
    14581 gacggatgat ttccgggtta ttggaaatct cctgcggacg caacacaatg cggctggcaa
    14641 agtaatccat attgtcgtaa atcttgcgca gggaatcggc cgagattgtc aggctggagg
    14701 cgctggcccc gctgattttg ccggtttcca gtaaatgcac caccgattcc tgtagcactt
    14761 ccgaatacat catgaacgga ggaatttccg ggttttcccc cagacgtgcc attaccgcat
    14821 tattgatatt gcccacgcca ctttgcagcg gcagaaattc cggcggaata cgcccatgcg
    14881 ccatttcctg caataagaac gtgaccacgt tatcggcaat ctgctggcac atgggatttt
    14941 gcttatccag catattaccg gcgtcgggca agttggtttc cacgacggcg acaatctttt
    15001 tcggatcgat ttgcacatag cgggtaccga cgcgatccat tgcatggaag atcgacacgc
    15061 tattgcgccg cggtggcgcg ccaggaatca caatatctgc cagttctgca acgcgcggat
    15121 cgtgatagtg attaagttca atgatcactt tcttcgctcg cagcagccag gtcggcgcat
    15181 taccgatccc gctggttaac cagactcgac catccggtgc cagtgccgat gcttcaatga
    15241 cggcaacatc aatgtcgccg aagaaaccgt aattgaccat ttgcgccact tcgctcaaat
    15301 gcaggtcaac gaaactcacc gcgccctgat tgatcttttt acgtaaactg gacgatgttt
    15361 gatatggcgc acgccaggaa acagcatcgg cgtcagaaag tacatcgtca gcggcggcgc
    15421 tgattgacgc ccccgtcagc aggcgaattt gatacggctt tttggcctca tgctgttcgt
    15481 tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga acccgctggg gtaaagccgc
    15541 taaatgccac catgtcgtta tgctggataa tttctgccgc ttcatcggcg gtcatccttg
    15601 tccactgagt ttccattttt actccttcgt tcgcagggat taatgaccaa cgaaattagg
    15661 tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt cataaaccgc
    15721 gcggcgcatc ccctgaatac gttcaaattc atcggagttc atggtgtgtg cttcgcccag
    15781 tacacgcagc tcttctttga taacggcaat ggctaacggc gctttctcag agatgtggtg
    15841 cgccatttgt aaggtgaaat cttccacttc cacaacatgg ttgaggatgc cgacagccag
    15901 cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc tctttgacaa tgtggaagcc
    15961 cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga ggtttacagg
    16021 cgtcattgag aaggttgagg tactggcggc gatgatcaga tcggaactca tgatcatttc
    16081 aaatgcgcca ccccaaacac taccttccac catcgaaatg atcggtttcg ggaatttttg
    16141 gatcatgcgg gtgatttgac gcaatggatc atcataggag agcggatcgc gaccgccaga
    16201 cggcagttcg tgaatatcgt gacctgcgga gaagactttg gatccacttg gcgcccgcaa
    16261 aatgatacag cgaatttccg gccggttgag atcgcttaac gcctgcataa gatcatcaat
    16321 aaagacttta cttaaggcat taagttttcg tccatagtta aactcaatga ccgccacttt
    16381 gttgatagtg gcaacgttca catactgata agacataaaa attcctttaa tcaaaatatt
    16441 gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc gtattgtttt
    16501 tgaccgctaa aagcgtctgg tggtaatagc gatcgaaatc ttcattcgcg aacaggtgat
    16561 tcagtacttc ttcttcggtc tgcttacgca gccattccac cgattgttgt tgccgcactt
    16621 gttgtaaacg accactggca gttagcgcgg ttttgaagtc gatgatggcg tgccagatct
    16681 catcgattcc acgtttttcc agtgcgctac aagtcagacc ccgtggctgc cattcgtcgt
    16741 atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg gcgacattgg
    16801 tatggttatc gccatcgtct ttgttgataa cgagcagatc agccacttcc atcagccctt
    16861 ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata aaacagtcca
    16921 ccatgcgggc gacttccgtt tccgactgcg cgacgccaac cgtttcgaca atcactacgt
    16981 cataacccgc cgcttcgcat aacagcatta attcccgtgc tcgctgactg gcaccgccca
    17041 gatgaccgga ggagggtacc gggcgaataa acgccgcctc ggcacgcgcc aggtcattca
    17101 tgcgggtttt atccccgaga atgctaccgc cagtgaccgg gctgctggga tcgaccgcaa
    17161 taacagcaac ctttaatccc tcacgaatca acaacatgcc aaaggcttca agaaaggtac
    17221 ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag ggcgttacca cagaacggca
    17281 taatagcgtc aagcagctgt gtacttagcg cctgatgacg tgggtgacgg ctttccacca
    17341 gcgtcatggc ttgggcgagc gtggcacgct caccctgacg taagcggcga atactttctg
    17401 ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc gcgcacactg
    17461 tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg caggaaggcg
    17521 taatcctgcg gcggaatgac gccacccgcg accacgcaga tatcttcgcg tccccatttt
    17581 ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag tgaggatgcg
    17641 cccactacgt gaacgtcgtt ttctacggcc aggcgggcga tctcttcagg tgtagagaac
    17701 atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat cactttcgcg
    17761 ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa tgcgcgggcg acgaccattg
    17821 tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc cgatttctca
    17881 gactgatgat agctttgcgc aatcacgccg gtaacacact ggcttggcac cagataacgg
    17941 tcgaaagcga cttccagcgc atcggaaatt tcacccaggg tggcgcgaac gcgagcggca
    18001 ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag ggcgttcaac
    18061 gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga agcaatttgc
    18121 tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc gtgatccagt
    18181 ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga ctgttcgcgc
    18241 gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt cgccatgcca
    18301 ccggcttcgt cgatctgttg gataatagct ctggcttgtt tgacgatttg atcggtcagc
    18361 gactcaatgt aataggatcc ggccagtgga tcgacggtgt ggcagagttc tgattcttcc
    18421 tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg caaaccaagc
    18481 gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc agccagtgct
    18541 tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct ccagcctgag
    18601 gtctggcagt gggtacgcag cgccagtgat ttcggatcct gtgcgccaaa tccactgacc
    18661 gcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa cagatccatg
    18721 ccgataccga agaataacga caggcgagga gcgaagtcat caattttcag tccggcagag
    18781 attgctgctt tgatgtactc aatcccatcg gcgatcgtaa atgctacctg ctgcacgcag
    18841 ttggcacccg cttcccccat gtggtaaccg ctgatactga tggtattaaa tcgcggcatg
    18901 ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt tggtgggtaa
    18961 atataggtgt tgcggcagag gtactctttg agaatatcgt tttgaatggt gccggtcagt
    19021 ttatcaggtg taacaccttg ctcttctgcg gcgacgatat aaaacgccag tactggtagc
    19081 actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg gtcgaacagg
    19141 actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc acccgccacg
    19201 cgtgggttat cggagtcgta gccacggtgg gtggcaaggt caaacgcaac ggaaagacct
    19261 ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc tgttgaaaaa
    19321 ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg cgggccacga
    19381 acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag atcggcttcg
    19441 gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga gtcgacagtt
    19501 ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgctc gttagacatt
    19561 atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cttacggtga gctttttacg
    19621 tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc ctgagatttg
    19681 tctattttgt gatgtgattc aaggaataca aaaagaagtg aggacaacgc ctgatatgtg
    19741 ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca taatcgagta
    19801 acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc cagtagagca
    19861 tccgtcgttg aaatagccca ggcgttaagt caatcagttc accgctggcc agctctttct
    19921 cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt tgtacgaacg
    19981 cttctgaaga attaacgata tggcagggta cgctgccagg aggaagatcg aagttttgct
    20041 gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc gctttcaata
    20101 atgccgaacg cgttacgccg ttaggaaaat atttttcggc aaacggtttt gagctgacga
    20161 acagatagtc gagcgcaccg agtttatcga caagacaact cggcagcgcc tgatgttgaa
    20221 tactcaccgc gccgaccact tcgccgcggc gcagacgttc ctgagtacgg gtttcatctt
    20281 ctacctgcaa gttgaggcgg ataggcgaat cagccaacac aggagccagt gccggaagca
    20341 accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag cagcagcgga gtcgaaccgg
    20401 tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc agtgccagca
    20461 gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc ggctgcccga
    20521 acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg cacagctttt
    20581 gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt aatgttctgt
    20641 agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct gcactatgac
    20701 acaattttgt gtcaggttgc atataccgaa gccgtcggcg ggtaacattt gtatcaatgg
    20761 ttaaacgcgc agcttaaaag atcagggatt tgcgctattg catttcgctt agcgacttcg
    20821 cgccctggtt atgttgaatg gcgtggcgtt attgcctctg ttcgcaccgt ggagcggcat
    20881 acccagaccg ttgtatgctt tatttgcggg gcgaaagggg atgcccgcca ttgcgcgata
    20941 ttttatggat gagttaacca cccagcttgc caacggggtc tgaatcgctt tttttgtata
    21001 taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat gaattgaaaa
    21061 aagcagtagg atgggcggca cttcagtatg ttcagcccgg taccattgtt ggtgtaggta
    21121 caggttccac cgccgcacac tttattgacg cgctcggcac gatgaaaggc cagattgaag
    21181 gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc attcacgttt
    21241 ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat gaaatcaacg
    21301 gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt
    21361 cggttgcgga aaaatttatc tgtattgccg acgcctccaa gcaggttgat attctgggta
    21421 aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg cgccagctgg
    21481 tgaaactggg cggtcgtccg gaataccgtc agggcgtggt gaccgataat ggcaacgtga
    21541 tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac gccataaatg
    21601 cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac gttgcgctga
    21661 ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga acctcccccg
    21721 ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgcttttacc aggcaattgt
    21781 cgattgctct aaataaatcc tctaaaccag catattcatc caagaattac ctttgcgtga
    21841 tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt tttgatatgt
    21901 tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga caggattggg taaatggcaa
    21961 aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg
    22021 cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa ggcgcgctgg
    22081 atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta
    22141 cccatctgac tgaagacgtg atcaacgccg cagaaaaact ggtcgctatt ggctgtttct
    22201 gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta
    22261 acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc
    22321 tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg aacaaactgg
    22381 cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg
    22441 gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg
    22501 aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata
    22561 tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat atgatgggcg
    22621 cgaaagagat ttcgctaatg aagcccggct cgctgctgat taatgcctcg cgcggtactg
    22681 tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatctggcg ggggcggcaa
    22741 tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct ccactgtgtg
    22801 aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga
    22861 atatcggctt ggaagttgcg ggtaaattga tcaagtattc tgacaatggc tcaacgctct
    22921 ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg tggtcgtcgt ctgatgcaca
    22981 tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatcttcgcc gagcagggcg
    23041 tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat gggttatgta gttattgata
    23101 ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta
    23161 ccattcgcgc ccgtctgctg tactaattcc ccttctctga aaattaacgg gcaggtcact
    23221 gacttgcccg tttttttatc ccttctccac accgttgccg cgttgcgcat tctactttgc
    23281 gagtctgctc cagaaaataa atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga
    23341 tttacgatgg cgggacagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
    23401 aaaggagaag ggttatgatg cgacttgtca tcatactgat tgtactgtta ctgataagtt
    23461 tcggagctta ttaacagtca gtctcaaggg aggagtcatc ctcccttacc cttactcact
    23521 aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg cggggtgcat cgccctgaac
    23581 cgaacttttt gatagccact tcatcgccca cgtttactgc cgtaatagat cctttttccc
    23641 aaaaattatc tctgttttgc atggggtttt tcgagcacgc tcgaagaaaa gatttaaagg
    23701 tagatcaatg cgttgtggtt atttaaagag agcttacgat agctgggcag catggggctg
    23761 taacggtaaa gccctcaccg aagcgagggc ttgaaggaga agggttatga tgcgacttgc
    23821 catcatactg attgtactgt tactcataag tttcccagct tattaacagt cagtcacagg
    23881 ggaggagcaa tcctccctta cccttactca ctaaattagg ttaaagaatc aacgatgtca
    23941 atcagggcga tgcaggtgta tcgcccttac cactcccaga ctttcgacgg tgtaaccacc
    24001 gcaggaagag ggatatccca ctcttcaacg gggagttttt ccaccaactg acaatcatgc
    24061 gcataaccca ccggttgcgt tttatagtgc tgccagtttt gtaaggtccg atcataaaaa
    24121 ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg cgaccagcgg tgtgatcagc
    24181 acgtctaatc gggaaagggg tagcacgtca cgcacatcca gttttggctc atggatcttc
    24241 aacctgttca tcaccagttc gctttgcgga tggtaattca ggaacagcaa attaccggca
    24301 ctaaagggat gcaaaactgg aagatatacg cgcttaccgg cgcgccagag ttgttctatt
    24361 agcggctggg tgtcgagttc accatcaaaa gagaggaata cagcgaccgt atgcgccatc
    24421 accaccgggg gataagtcat catccgggta gcggcttgtt gacccatttc cagctgttgt
    24481 tccggcgtta acgcacgacg acgttgccga atcattttgc ggatttcttg tcgggataat
    24541 gtcagtggga gctctggtag ttgcgtcatg gctggtgcca gataagaagg gaatctccga
    24601 gatgccgccg caggctgtaa cccttgaacc cttggttcaa ggtgaatgtg tcgtcgcagt
    24661 tttaaggctt ctcggacgaa ccgagcatgc tcaccaaccg cggagcgcca cattcttgtg
    24721 gtatgaaata tcggctcagg ggactggccc gcttgcgaac atctcagaga aattttgtct
    24781 tcacggttac tctaccacag taaaccgaaa agtgtcattc aaagttttgg ttagtttttt
    24841 cggtgatgcg accttgttca agtaacgctt gttctatggt ctgctgcagc atccgaatac
    24901 gctgttccat acttgccgcg tagtcacgag tctttgcttt ttcttgcgct aactcatagc
    24961 tgatattcaa tgcggcaatg aagaccaact gttcagtatt tgtgactcta gtgcgttctt
    25021 tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg attcaacgca tccctttggt
    25081 caggcgggca gttcacacgc agtgaacggc caaaaatttg gatatcgacg ggttgtgcag
    25141 acatgccacc ttcctgctga ttgactgcgc tgccttcgtc tttagaccca tggtctgcga
    25201 aggggcgaca ctatagctac cctgatgaga agagacaagc ccttttctgg tccaccaggg
    25261 cccaaagtgg tagcatatca tgaatattcc tccctttgac gacgaatgct tatgtctata
    25321 cagaacgaaa tgcctggtta caacgaaatg aaccagtatc tgaaccaaca agggacgggt
    25381 ctgacccctg ccgagatgca tggtttaatc agtgggatga tatgtggcgg taacgatgac
    25441 agctcatggc tgccgctact tcacgacctg acgaacgaag gcatggcttt cggtcatgag
    25501 ctggcacagg cactgcgtaa aatgcactct gccaccagcg atgccctgca ggatgacggc
    25561 ttcctttttc agctttatct gcctgatggc gatgatgtca gcgttttcga tcgggctgat
    25621 gcactggcag gttgggtcaa tcacttcctg cttggtcttg gcgttacgca accgaagctg
    25681 gacaaagtga ccggcgaaac cggtgaagcc atcgacgatc tgcgtaacat cgcgcagctg
    25741 ggctacgacg aagacgaaga tcaggaagag cttgaaatgt cgcttgaaga gatcatcgaa
    25801 tacgttcgtg ttgccgcgct gttatgccac gacaccttta ctcatccgca accgaccgcg
    25861 ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa cgtaaggaga gtgttatgag
    25921 tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc ctggtggagc aaatgcaacc
    25981 cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca cgtagcgccg acagcgaata
    26041 cccctatcgt cagaacagtg acttctggta cttcaccggc tttaacgaac cggaagcggt
    26101 gctggtgctg attaaaagcg atgacactca taaccacagc gttctgttta accgcgttcg
    26161 cgacctgacg gcggagatct ggtttggccg tcgcttaggc caggatgccg cgccagagaa
    26221 actgggcgtt gaccgcgcac tggcattcag cgaaatcaat cagcaacttt atcaactact
    26281 taacggcctg gatgtggttt accatgccca gggcgaatat gcatatgctg atgaaatcgt
    26341 gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa aatctcaccg caccggcaac
    26401 gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg ttcaaatcgc cagaagagat
    26461 tgccgtactc cgccgcgcgg gagaaatcac cgccatggca catacacggg cgatggaaaa
    26521 atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa attcaccacg aatttaaccg
    26581 ccacggtgcg cgctatcctt cctataacac cattgtaggc agcggtgaaa acggctgcat
    26641 tctgcactac accgaaaacg agagtgaact gcgcgacggt gacctggtgt tgattgacgc
    26701 gggttgtgaa tacaaaggtt acgcgggcga tattacccgc accttcccgg tcaacggcaa
    26761 attcacccag gcccagcgtg aaatctacga cattgtgctg gagtctctcg aaaccagcct
    26821 gcgcctgtat cgtccgggaa cttccattct ggaagtcact ggtgaagtgg tgcgcatcat
    26881 ggttagcggc ctggtaaaac tcggcatcct gaaaggtaat gttgatgaac tgatcgctca
    26941 gaacgcccat cgtcctttct ttatgcatgg ccttagccac tggttaggac tggatgtcca
    27001 tgacgttggc gtttatggtc aggatcgctc gcgcattctg gaaccgggga tggtactgac
    27061 cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg ccagaacaat atcgcggtat
    27121 cggcattcgc attgaagacg acattgtgat taccgaaacc ggcaacgaaa acctcaccgc
    27181 cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg gctgctgcga gaaagcaatg
    27241 agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc tggcgctggc tatttcccgg
    27301 ttaagtcacg gggcgctgcc ggtacatttg attgaagcga ctgcgccaga gtcacatgct
    27361 catccgggct ttgatggacg agcgatagcg ctggcggcgg gtacctgtca gcaactggcg
    27421 cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg ccatcactac cgtgcatgtc
    27481 agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag aagattacca actggcggcg
    27541 ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc tgtttgcatt gctgcgaaaa
    27601 gcacctggcg taatgctgca ttgccccgat cgcgtagcta acgttgcccg tactcagagt
    27661 catgttgaag tgacgctgga gagcggcgag atgctgacgg gccgcgtgct gatagccgct
    27721 gatggcaccc attcagcgtt agccaccgcg tgcggcgttg attggcagca ggagccttac
    27781 gaacaactgg cagtgattgc caacgttgct acttctgttg cgcacgaagg ccgcgctttt
    27841 gaacgcttca cgcaacatgg cccgctggcg atgttgccga tgtctgacgg acgctgttcg
    27901 ctggtctggt gtcatccact ggaacggcgc gaagaggtgc tgtcgtggag cgacgagaag
    27961 ttttgccgtg aactccagtc tgcctttggc tggcgactgg ggcaaattac ccacgccggt
    28021 aaacgcagtg cttatccgct ggcattaacc cgcgccgcca gaccgattac ccatcgcact
    28081 gtgctggtgg gcaatgcggc gcaaactctg cacccgattg ccgggcaagg gtttaacctc
    28141 ggtatgcgag atgtcatgag ccttgcggaa accctgactc aggcgcatga gcgcggagaa
    28201 gacatcggtg attacggcat attgtgccgc tatcagcagc gtcgacagag cgatcgcgaa
    28261 gcaaccatcg gcgtcacgga cagcctggta catctttttg ccaaccgttg ggcaccgctg
    28321 gttgtcgggc gcaacatcgg actgatgacg atggaattat tcaccccggc acgcgatgtg
    28381 ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc ggttttaagg aataaacaat
    28441 gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg gggctggcgg ttgcctgcgg
    28501 cttacagggg agcggcttac gcgttgccgt actggagcag cgcgtaccgg aacctctggc
    28561 ggtagatgca ctaccacaac tgcgcgtttc ggctatcaat gccgccagcg aaaaattact
    28621 cacccgtctt ggcgtctggc aggacattct cgctcgtagg gccagttgtt atcacggcat
    28681 ggaagtgtgg gacaaagaca gttttggtca catctcgttt gacgatcaaa gcatgggcta
    28741 tagccatctt gggcacatcg ttgaaaactc agtgattcac tacgcgctgt ggaacaaagc
    28801 gcagcagtcg tcagatatta ctctgttggc ccccacagaa ttacagcagg tcgcctgggg
    28861 agaaaatgaa accttcctga cgctgaaaga tggcagcatg ttaacggcgc gtctggtgat
    28921 tggcgcggac ggtgctaatt cctggttgcg caacaaagca gatattccgc tgactttctg
    28981 ggattatcag catcacgcgc tggtagcgac cattcgcacg gaagaaccgc atgatgcggt
    29041 ggcgcggcag gttttccatg gcgaaggcat tctggccttt ttaccgctta gcgatccgca
    29101 tctttgctcg attgtctggt cactgtcgcc agaggaagcg cagcggatgc agcaggcaag
    29161 tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat aatcgccttg gcttatgcaa
    29221 ggttgagagc gcgcgtcagg tgttcccgct gacggggcgt tatgcgcgcc agtttgccgc
    29281 acaccgtctg gcgctggtgg gcgacgccgc gcataccatt cacccactgg cggggcaggg
    29341 cgttaatctc ggatttatgg acgccgcaga gctgattgct gaactgaaac ggttgcatcg
    29401 tcagggtaaa gacatcgggc agtacattta cctgcgccgc tatgaacgta gccgcaagca
    29461 cagcgcggcg ctgatgctgg ctggtatgca gggttttcgc gatctctttt ccggcgctaa
    29521 tccggcgaaa aaactgctgc gtgatattgg tttgaaactg gccgacacgc ttcctggcgt
    29581 taagccgcaa cttatccgcc aggcaatggg attaaacgat ttgcctgaat ggctgcgtta
    29641 aaaatttctc ctctgttgtt tatttgatac ccatcacact ttcatctccc ggttttttcg
    29701 ccgggagatt ttcctcattt gaaataaact aatttcacct ccgttttcgc attatatttt
    29761 ctaataccat gattttttga tttagtgttt tttgacattt ttttagctct taatattgtc
    29821 ttattcaaat tgactttctc atcacatcat ctttgtatag aaattggtgt attttttggt
    29881 tttttattct gtcgcgattt ttgcattttt taaccataag ctaatgtgat gatcaatttt
    29941 accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac gattgcgttg
    30001 gggaccggga gtggctccga tgctgggttt tgtggtgata atttcaccat gaaaaagttg
    30061 tcagccccgc ttattcaatg aggacaagat ggcacaacag actcctttgt acgaacaaca
    30121 cacgctttgc ggcgctcgca tggtggattt ccacggctgg atgatgccgc tgcattacgg
    30181 ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc ggaatgtttg atgtgtcgca
    30241 tatgaccatc gtcgatctcc gcggcagccg cacccgggag tttctgcgtt atctgctggc
    30301 gaacgatgtg gcgaagctca ccaaaagagg caaagccctt tactcgggga tgttgaatgc
    30361 ctctggcggt gtgatagatg atctcatcgt ctactacttt actgaagatt tcttccgcct
    30421 cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg attacccaac acgctgaacc
    30481 tttcggcatc gaaattaccg ttcgtgatga cctttccatg attgccgtgc aagggccgaa
    30541 tgctcaggca aaagctgcca cactgtttaa tgatgctcag cgtcaggcgg tggaagggat
    30601 gaaaccgttc tttggcgtgc aggtgggcga tctgtttatt gccaccactg gttataccgg
    30661 tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg gccgatttct ggcgtgcgct
    30721 ggtggaagcg ggcgttaagc cctgcggtct gggggcgcgt gacacgctgc gtctggaagc
    30781 gggcatgaat ctttatggtc aggagatgga cgaaactatt tctcctttag ccgccaacat
    30841 gggctggact atcgcctggg aaccgacaga tcgtgacttt atcggtcgtg aagccctgga
    30901 agtgcagcgt gagcatggta cagaaaaact ggttggtctg gtgatgaccg aaaaaggcgt
    30961 gctgcgtaat gaactgccgg tacgctttac cgatgcgcag ggcaaccagc atgaaggcat
    31021 tatcaccagc ggtactttct ccccgacgct gggttacagc attgcgctgg cgcgcgtgcc
    31081 ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac cgtgaaatgc cggttaaagt
    31141 gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg tgatttactt ttttggagat
    31201 tgattgatga gcaacgtacc agcagaactg aaatacagca aagaacacga atggctgcgt
    31261 aaagaagccg acggcactta caccgttggt attaccgaac atgctcagga gctgttaggc
    31321 gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg ttagcgcggg cgatgactgc
    31381 gcggttgccg aatcggtaaa agcggcgtca gacatttatg cgccagtaag cggtgaaatc
    31441 gtggcggtaa acgacgcact gagcgattcc ccggaactgg tgaacagcga accgtatgca
    31501 ggcggctgga tctttaaaat caaagccagc gatgaaagcg aactggaatc actgctggat
    31561 gcgaccgcat acgaagcatt gttagaagat gagtaacggc tttattcctc ttctgcggga
    31621 gaggatcagg gtgaggaaaa tttatgcctc accctcactc tcttcgtaag gagagaggtt
    31681 cacaattcac tgcacgtttc aggaaccatc gctcatgaca cagacgttaa gccagcttga
    31741 aaacagcggc gcttttattg aacgccatat cggaccggac gccgcgcaac agcaagaaat
    31801 gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc ggccagattg tgccgaaaga
    31861 tattcagctt gcgacaccac cgcaggttgg cgcaccggcg accgaatacg ccgcactggc
    31921 agaactcaag gctattgcca gtcgcaataa acgcttcacg tcttacatcg gcatgggtta
    31981 caccgccgtg cagctaccgc cggttatcct gcgtaacatg ctggaaaatc cgggctggta
    32041 taccgcgtac actccgtatc agcctgaagt ctcccagggc cgccttgaag cactgctcaa
    32101 cttccagcag gtaacgctgg atttgactgg actggatatg gcctctgctt ctcttctgga
    32161 cgaggccacc gctgccgccg aagcaatggc gatggcgaaa cgcgtcagca aactgaaaaa
    32221 tgccaaccgc ttcttcgtgg cttccaatgt gcatccgcaa acgctggatg tggtccgtac
    32281 tcgtgctgaa acctttggtt ttgaagtgat tgtcgatgac gcgcaaaaag tgctcgacca
    32341 tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact accggtgaaa ttcacgacta
    32401 cactgcgctt attagcgaac tgaaatcacg caaaattgtg gtcagcgttg ccgccgatat
    32461 tatggcgctg gtgctgttaa ctgcgccggg taaacagggc gcggatattg tttttggttc
    32521 ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca cacgcggcat tctttgcggc
    32581 gaaagatgaa tacaaacgct caatgccggg ccgtattatc ggtgtatcga aagatgcagc
    32641 tggcaatacc gcgctgcgca tggcgatgca gactcgcgag caacatattc gtcgtgagaa
    32701 agcgaactcc aacatttgta cttcccaggt actgctggca aacatcgcca gcctgtatgc
    32761 cgtttatcac ggcccggttg gcctgaaacg tatcgctaac cgcattcacc gtctgaccga
    32821 tattctggcg gcgggcctgc aacaaaaagg tctgaaactg cgccatgcgc actatttcga
    32881 caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg acgcgtgccg aagcggctga
    32941 aatcaacctg cgtagcgata tcctgaacgc cgtggggatc acccttgatg aaaccaccac
    33001 gcgtgaaaac gtaatgcagc ttttcagtgt gctgctgggc gataaccacg ggctggagat
    33061 cgacacgctg gacaaagacg tggctcacga cagccgctct atccagcctg cgatgctgcg
    33121 cgacgacgaa atcctcaccc atccggtgtt taatcgctat cacagcgaaa ccgaaatgat
    33181 gcgctatatg cactcgctgg agcgtaaaga tctggcgctg aatcaggcga tgatcccgct
    33241 gggttcctgc accatgaaac tgaacgccgc cgccgagatg atcccaatca cttggacgga
    33301 atttgccgaa ctgcacccgt tttgcccgcc ggaacaggct gaaggttatc agcagatgat
    33361 tgcgcagctg gctgactggc tggtgaaact gaccggttac gacgccgttt gtatgcagcc
    33421 gaactccggc gcacagggcg aatacgcggg cctgctggcg attcgtcatt atcatgaaag
    33481 ccgcaacgaa gggcatcgcg atatctgcct gatcccggct tctgcgcacg gaactaaccc
    33541 cgcttctgca catatggcag gaatgcaggt ggtggttgtg gcgtgtgata aaaacggcaa
    33601 catcgatctg actgatctgc gcgcgaaagc ggaacaggcg ggcgataacc tctcctgtat
    33661 catggtgact tatccttcta cccacggcgt gtatgaagaa acgatccgtg aagtgtgtga
    33721 agtcgtgcat cagttcggcg gtcaggttta ccttgatggc gcgaacatga acgcccaggt
    33781 tggcatcacc tcgccgggct ttattggcgc ggacgtttcg cacctcaacc tgcataaaac
    33841 tttctgcatt ccgcacggcg gtggtggtcc gggtatggga ccgatcggtg tgaaagcgca
    33901 tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc gaaggcatgt taacccgtca
    33961 gggcgcagtt tctgcggcac cgttcggtag cgcctctatc ctgccaatca gctggatgta
    34021 catccgcatg atgggcgcag aagggctgaa aaaagcaagc caggtggcaa tcctcaacgc
    34081 caactatatt gccagccgcc tgcaggatgc cttcccggtg ctgtataccg gtcgcgacgg
    34141 tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg aaagaagaaa ccggcatcag
    34201 cgagctggat attgccaagc gcctgatcga ctacggtttc cacgcgccga cgatgtcgtt
    34261 cccggtggcg ggtacgctga tggttgaacc gactgaatct gaaagcaaag tggaactgga
    34321 tcgctttatc gacgcgatgc tggctatccg cgcagaaatt gaccaggtga aagccggtgt
    34381 ctggccgctg gaagataacc cgctggtgaa cgcgccgcac attcagagcg aactggtcgc
    34441 cgagtgggcg catccgtaca gccgtgaagt tgcggtattc ccggcaggcg tggcagacaa
    34501 atactggccg acggtgaaac gtctggatga tgtttacggc gaccgtaacc tgttctgctc
    34561 ctgcgtaccg attagcgaat accagtaatt cactgattcg agtattttct aaaggcgctt
    34621 cggcgccttt ttagtcagat gacaaagtac aaaagtgctc agacagtccc ctcgcccctt
    34681 tggggagagg gttagggtga ggggaacagg ccggcactgg cgcgaatatt taccctcacc
    34741 ccgaccctct ccctgaaagg gcgaggggga aaagcgtgcc aacattgaag attgagccag
    34801 tttgttagca atctcaaaga tacgtcaacg aattaatttt tctcggaaaa acaaatggct
    34861 atagcacttg tgaccggtgg cagtcgcggc atcgggcggg caactgcatt actgttggcg
    34921 caagaagggt atacggtggc ggttaattat cagcaaaacc tccacgcggc gcaggaagtg
    34981 atgaacttaa taatgcaagc cggtggcaaa gcattcgtgc tccaggcgga tatcagcgac
    35041 gaaaaccagg tcgttgcgat gtttacagca atcgatcagc acgatgaacc gctagcagcg
    35101 ctggtcaata acgccgggat cttgtttacc cagtgcaccg ttgaaaacct taccgcagag
    35161 cgaatcaacc gagtactttc caccaacgtg acgggatatt ttctctgctg ccgcgaggcg
    35221 gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg ctatcgtcaa tgtctcttcg
    35281 gtggcctcac ggttgggttc gccaggggaa tatgttgatt acgcggcatc gaaaggggcg
    35341 attgattcgt taaccaccgg actatcgctg gaagtcgccg cgcaggggat ccgcgttaac
    35401 tgcgtgcggc cagggtttat ttataccgaa atgcacgcca gcgcggcgag cctggacgcg
    35461 tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg acaggcagaa gaggtcgcgc
    35521 aggccattgt ctggctacta agtgataaag cctcttacgt cacgggaagt tttatcgatt
    35581 tggcgggcgg gaaataaaac agggaagttg tctgaccgga tgcaacaagt attgcatccg
    35641 gtacttcatc gacttaaagc ttctcgccgt tgctggcaat cacctctttg taccagttaa
    35701 agctcttctt acgtgaacgc gacatatcgc cagtaccgtc gtcatgttta ttcacataga
    35761 taaagccgta gcgtttgctg tactgcccgg tagtgaatga cacgcagtcg atgcagcccc
    35821 acggtgtgta gcccatcaga tccacaccat cgtaagtcac tgcttttttc atctcttcaa
    35881 tatgggcgcg caggtagtca atgcggtagt cgtcgttgat gctgccatct tcttccactt
    35941 tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg cctctgataa cgctcataca
    36001 gttcgcaaag ggcatagcgc agacctactg gatcaatctg ccagccccag tcagacgctt
    36061 taacatacgg gttcggtacg ctgccttcaa aaccagagat cgcatcgccg gtgccgcctt
    36121 cggccttcac tgcgttggtc atgtaatagc tgaaaccaag ataatcgcag gtgccttcgc
    36181 gcagtacatc gagatcgccg tcttccattt tgatattaaa tccgcgacgt tcccactcgt
    36241 tcaatacata ggacgggtaa tagccgcata gctgcacatc ggtaaagacg tagcgttcgc
    36301 gcatcgactc ctgagcgaac atcacatcgt ccgggttaca tgagtaggga tagagcggca
    36361 ccatcgccag catacagccg actttcatct ccgggttaat gcgacgcgcg gctttcactg
    36421 ccagggcgct ggcgacaaac tggtgatgca gcacctggta catcgtctct tccgggttgt
    36481 catgctcggt atacaccacg ccggagcagc agtaaccaaa cagcggcgca cgccagttac
    36541 gctggttgtt tatttcgttg aaggtcatcc agtatttgac tttatgcttg tagcgttcga
    36601 ataccacttc cgcgaaacgg acaaagaaat caacgaattt acggttggtc cagctaccgt
    36661 attgctgcac cagatgcagc ggcatttcaa agtgggagag ggttatcacc ggttcgatgt
    36721 tgtatttcag cagttcatcg aacagagagt cgtaaaactt cagcccttct tcgtttggct
    36781 gagcttcatc accttttgga aaaatacgcg tccaggcgat ggaagtccga aaacatttga
    36841 agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg accataaaaa tcaacggctt
    36901 catggtttgg atagtatttt cctggcaaga cttctttggt gatttcgcgc ggcacgccgt
    36961 gtgcgccacc ggtcagaacg tcacaaatgc tcggtccttt gccgcctttg ttccagccgc
    37021 cttcgacctg atgagcggca acggcgccac cccataagaa atctttcggt aaggtgagtt
    37081 ttttcactat cataggctcc agatcagtca attcttacag gaaatagtac catgcagcca
    37141 aacgacatca cttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca
    37201 ccatccgcga cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcggacgtt
    37261 ttgaagatga cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaacgctgg
    37321 atacactgac ggaaaaacat gcagagcagg aaaatatgac cctgcctgaa ctgaaaaagg
    37381 tcattgctga catctatccc ggtcagacac aattttatat gattgaattt aaatgtcttt
    37441 aaattgaact cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt tttaggagat
    37501 tagtctcaaa tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac
    37561 gctggagagg tatacatgtg cgtgtacgtt tctctggagt aagttatggt tcagaagccc
    37621 ctcattaagc agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt
    37681 gggctggtgt ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat
    37741 gccagcgcca cagcgataac cagttacagc ctctatggcg gcagtatgat cctgctgttc
    37801 ctcgcttcga cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa
    37861 tttgaccatt gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg
    37921 gggctggatt ctccgttagc gcgcgggttg atgattgtta tctggagcct agcattgctg
    37981 gggattctgt tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacttat
    38041 ctggcgatgg gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg
    38101 ggcagcgtta ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac
    38161 gtctgcaaac gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt
    38221 gtgtgccact ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt
    38281 aggcctgata agacgcgtca gcgtcgcatc aggcatcgga gcgcaaccgc cggatgcggc
    38341 gtgaacgcct tatccgatct acgggcctcg tccctgttgt gataaaggca tcaaatccca
    38401 gacatgataa gacgcgtcag cgtcgcatca ggcgataaat gcctgatgcg atacagagat
    38461 ttactcttcg agcgaatacg gcagcggctc gatatgcagt gtattcgcat cgtcgcgtac
    38521 gcggaagatg ctgtccggtt ccatatcgtt attcatgacg acctgtacca cgacctgacc
    38581 atcttccagt tttaccgcag ccagcaccgt accggtacga cgccagttct cgcccatttt
    38641 cagctctaag tcttcaccag cttccggcag tcggctgaca ctacctgcca gcaaccagag
    38701 cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc atctcttgtc cggtataaca
    38761 gcctttctta aagctgatac cgcccagcgc ctggagattg gtcgcctgtg ggataaactg
    38821 cccgctgttg gcggcatcaa tcaccgggaa accagcttca atgtttaatg ccagccactg
    38881 ttggctattg ttcagttccg cttcaccgcg cagtttatcg gtcagcatat tagcagtagc
    38941 ttcatcggtt acgatcagga aacgttctgc cgggtgttca aaccatagca aagtggtcgc
    39001 gccttctttg actacctgtt tttctttcga aggcagttcg ctaaagagat ttgccagcgc
    39061 ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg gcgcgatggt
    39121 cactttagag aataccgcat attttttcag ttcagtcagc tgcggttcac gcacgctgcg
    39181 ccgttcaatc catgcaaagc catcgccgtc gcggaacaga cgtaaattgc tccacatttt
    39241 acctttggcg tcgcaatggg cggcgagcag gtgctgatct tctgccatct ggctgacatc
    39301 tgctgtcacc tgaccctgca tatatttttc gctgtccgcg ccagtaatgg tggcaagcgc
    39361 ccagtcatca agcgtcatca gcgtcagcgg taaacgggca gaagccgtag gctgacgtgg
    39421 aggaaaaggt gtaaaagcca taatagagtc ctgagttgct taacggcgta ttaatgacta
    39481 atggtaaaag agctatgagg caatgcaagt gctttcacta cgcgatctgt gccttctgcg
    39541 aggcgactgc caaaaaaatg aagcccttga attgttgtga attaatttcg cgatcgcgat
    39601 ggtgtttact gatattcccg taaaagaagt cgaaaaacac gttacaatag ccgggtactg
    39661 gattttcgtg agacaggaag aagatggaca ttaacaacaa agcccgaatt cattgggcat
    39721 gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc gtttttcgaa catgagtacg
    39781 acagcttaag cgatgacgaa aaacgcatct ttattcgtct gctggaatgt gacgatccgg
    39841 acctgtttaa ctggctgatg aatcacggta aaccggccga tgcagaactg gaaataatgg
    39901 tccgactcat ccagacacgg aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc
    39961 tcctggcgcg cacagtggct ttccttgctg attcatgggc tggttgccgc tgttatttta
    40021 ctcatgccct ggccgctcag ttacaccccg ttatggatgg tgttactttc gctggtggta
    40081 tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc agggggaaat tcgcttgttg
    40141 atggacgggc gtttgcgttg gcaagggcag gagtggagca tcgtcaaagc accgtggatg
    40201 attaagagcg gcatgatgct gcgtttacgt tctgatggcg gtaaacggca acatttatgg
    40261 ctggcagccg acagcatgga cgaagccgaa tggcgggatt tacggcggat tttgttgcaa
    40321 caagagacgc aaagataaag atgacggcaa agcatatgcg ctaagccgtc agtgagatca
    40381 ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc
    40441 gctaaggtca tactggttag tttcatccag cgccagaccc acgaacagtt gcccgtcagc
    40501 aatcaccggt ttcgggctgg taaattcata tccttccgtt ggccagtagc cgacgaactt
    40561 cacgcctttg gtcgagagtt tgtcatgcag cataccgagc gcatcgagga accactcgcc
    40621 gtatcccagt tgatcgccaa gcccatacag cgcaataatt ttaccttcaa ggttcaggtc
    40681 gtcgagctga tcccagacgg cttcccagtc ttcctggatt tcaccaaaat cccaggtcgg
    40741 gatacccaga atgagcacat cgtactgctc cattaatttc ggggagtcgt ccttgaggtt
    40801 atgtaaggtc accagttctg ggccgataat atcgcggatt ttttctgccg ccatttcggt
    40861 gtaacaggtg ctggaaccgt aaaaaagacc catattcata acgtaagctt ctcgattctg
    40921 ctgtggcttt tgcagctagt gtatcagaat cgctttagtc acggcacaat aaggcataat
    40981 ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg agctggcacg catcgagcag
    41041 tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt
    41101 cgcgatctgt caatgatggt ggagtggttg catcaccgcg ggttgacgct ggcgacggcg
    41161 caaagtgacg atttgcaggc attactggca gaacggctgg agggcgggta taaagccacc
    41221 agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag
    41281 tttcgtgaag acgatcccag tgcgcatctc gcttcaccga aattgcccca gcgtttgcca
    41341 aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg cgccattaat tgatcagcca
    41401 ctggagctac gcgataaagc catgcttgaa gtgttgtatg ctaccgggct gcgtgtctct
    41461 gaactggtcg ggctgacaat gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt
    41521 ggtaaaggca acaaagagcg cctggtgccg ttaggggaag aggcggttta ctggctggaa
    41581 acctatctgg aacatgggcg tccgtggctg ctgaatggtg tgtcaattga cgtgttgttt
    41641 cccagccagc gtgcgcagca gatgacgcga cagaccttct ggcaccgcat taaacattat
    41701 gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc atgtgttgcg tcacgctttt
    41761 gccactcatt tattaaatca tggtgcggat ttacgcgtgg tgcagatgct actgggccac
    41821 agcgatcttt ccaccacgca aatttatacg catgtcgcta ccgagcgtct gcggcaactt
    41881 catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt tatgaagaaa
    41941 ggttttatgt tgtttacttt gttagcggcg ttttcaggct ttgctcaggc tgatgacgcg
    42001 gcaattcaac aaacgttagc caaaatgggc atcaaaagca gcgatattca gcccgcgccc
    42061 gtagctggca tgaagacagt tctgactaac agcggtgtgt tgtacatcac cgatgatggt
    42121 aaacatatca ttcaggggcc aatgtatgac gttagtggca cggctccggt caatgtcacc
    42181 aataagatgc tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg
    42241 ccgcaggaaa aacacgtcat caccgtgttt actgatatta cctgtggtta ctgccacaaa
    42301 ctgcatgagc aaatggcaga ctataacgcg ctggggatca ccgtgcgtta tcttgctttc
    42361 ccgcgccagg ggctggacag cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa
    42421 gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc
    42481 gacgtggata ttgccgacca ttacgcactt ggcgtccagc ttggcgttag cggtactccg
    42541 gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc agccgccgaa agacatgaaa
    42601 gaattcctcg acgaacacca aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga
    42661 tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt gcccgctgaa ttgcctccct
    42721 tgctgcgccg tttatacgcc agccggggag tacgcagtgc gcaagaactg gaacgcagtg
    42781 ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga aaaggccgtt gagatccttt
    42841 acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg tgatttcgac gccgacggcg
    42901 cgaccagcac ggctctaagc gtgctggcga tgcgctcgct tggttgcagc aatatcgact
    42961 acctggtacc aaaccgtttc gaagacggtt acggcttaag cccggaagtg gtcgatcagg
    43021 cccatgcccg tggcgcgcag ttaattgtca cggtggataa cggtatttcc tcccatgcgg
    43081 gagttgagca cgctcgctcg ttgggcatcc cggttattgt taccgatcac catttgccgg
    43141 gcgacacatt acccgcagcg gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc
    43201 cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct
    43261 ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc aattcctaac ctggcagaac
    43321 tgctggatct ggtagcactg gggacagtag cggacgtcgt gccgctggac gctaataatc
    43381 gcattctgac ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt ccagggatta
    43441 aagcgctgct ggaagtggca aatcgcgatg cacaaaaact agccgccagc gatttaggtt
    43501 ttgcgctggg accgcgtctc aatgctgccg ggcgactgga tgatatgtcc gttggtgtgg
    43561 cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct ggcaaatgaa ctcgatgcgc
    43621 taaaccagac gcgaaaagag atcgaacaag gaatgcaggt tgaagccctg accctgtgcg
    43681 agaaactgga gcgcagccgt gacacgctac ccggcgggct ggcaatgtat caccccgaat
    43741 ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa agagcgtttt caccgtccgg
    43801 ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg ttccggtcgc tccattcagg
    43861 ggctgcatat gcgtgatgcg ctggagcgat tagacacact ctaccccggc atgatgctca
    43921 agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa ttcgaactct
    43981 ttcaacaacg gtttggcgaa ctggttactg agtggctggc cccttcgcta ttgcaaggcg
    44041 aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac catggaagtg gcgcagctgc
    44101 tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc gctgtttgac ggtcatttcc
    44161 gtctgctgca acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc gaaccggtcg
    44221 gcggcggtcc actgctggat ggtattgctt ttaatgtcga taccgctctc tggccggata
    44281 acggcgtgcg cgaagtgcaa ctggcttata agctcgatat caacgagttt cgcggcaacc
    44341 gcagcctgca aattatcatc gacaatatct ggccaattta gcgtcatctt ctctataaaa
    44401 aagagcgtgg attgggtaca atcccgctct tatcaccgca ttttgactag ctcaataaaa
    44461 gaaatcagac catgtttgaa attaatccgg taaataatcg cattcaggac ctcacggaac
    44521 gctccgacgt tcttaggggg tatctttgac tacgacgcca agaaagagcg tctggaagaa
    44581 gtaaacgccg agctggaaca gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg
    44641 ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg
    44701 ctggaagatg tttctggtct gctggaactg gctgtagaag ctgacgacga agaaaccttt
    44761 aacgaagccg ttgctgaact cgacgccctg gaagaaaaac tggcgcagct tgagttccgc
    44821 cgtatgttct ctggcgaata tgacagcgcc gactgttacc tcgatattca ggcggggtct
    44881 ggcggtacgg aagcacagga ctgggcgagc atgcttgagc gtatgtatct gcgctgggca
    44941 gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt
    45001 aaatccgtga cgatcaaaat ctccggcgat tacgcttacg gctggctgcg tacagaaact
    45061 ggcgttcacc gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg ccacacgtcg
    45121 ttcagctctg cgtttgttta tccggaagtt gatgatgata ttgatatcga aatcaacccg
    45181 gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt
    45241 accgaatctg cggtgcgtat tacccacatc ccgaccggga tcgtgaccca gtgccagaac
    45301 gaccgttccc agcacaagaa caaagatcag gccatgaagc agatgaaagc gaagctttat
    45361 gaactggaga tgcagaagaa aaatgccgag aaacaggcga tggaagataa caaatctgac
    45421 atcggctggg gcagccagat tcgttcttat gtccttgatg actcccgcat taaagatctg
    45481 cgcaccgggg tagaaacccg caacacgcag gccgtgctgg acggcagcct ggatcaattt
    45541 atcgaagcaa gtttgaaagc agggttatga ggaaccaaca tgtctgaaca acacgcacag
    45601 ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg
    45661 aacctgcgtg agcagggaat tgccttcccg aacgatttcc gtcgcgatca tacctctgac
    45721 caattgcacg cagaattcga cggtaaagag aacgaagaac tggaagcgct gaacatcgaa
    45781 gtcgccgttg ctggccgcat gatgacccgc cgtattatgg gtaaagcgtc tttcgttacc
    45841 ctgcaggatg tgggtggccg cattcagctg tacgttgccc gtgacgatct gccggaaggc
    45901 gtttacaacg agcagttcaa aaaatgggac ctcggcgaca tcctcggcgc gaaaggtaaa
    45961 ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta ccgagctgcg tctgctgacc
    46021 aaagcactgc gtccgctgcc ggataaattc cacggcttgc aggatcagga agcgcgctat
    46081 cgtcagcgtt atctggatct catctctaac gatgaatccc gtaacacctt taaagtacgc
    46141 tcgcagatcc tctctggtat tcgccagttc atggtgaacc gcggctttat ggaagttgaa
    46201 acgccgatga tgcaggtgat cccaggcggt gccgctgcgc gtccgtttat cacccaccat
    46261 aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg aactgtacct caagcgtctg
    46321 gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa cgaaggtatt
    46381 tccgtacgtc ataacccaga gttcaccatg atggaactct acatggctta cgcagattac
    46441 aaagatctga tcgagctgac cgaatcgctg ttccgtactc tggcacagga tattctcggt
    46501 aagacggaag tgacctacgg cgacgtgacg ctggacttcg gtaaaccgtt cgaaaaactg
    46561 accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga tctggataac
    46621 ttcgactctg cgaaagcgat tgctgaatct atcggtatcc acgttgagaa gagctggggt
    46681 ctgggccgta tcgttaccga gatcttcgaa gaagtggcag aagcacatct gattcagccg
    46741 accttcatta ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa cgacattaac
    46801 ccggaaatca cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg taacggcttt
    46861 agcgagctga atgacgcgga agatcaggcg caacgcttcc tggatcaggt tgccgcgaaa
    46921 gacgcaggtg acgacgaagc gatgttctac gatgaagatt acgttaccgc actggaacat
    46981 ggcttaccgc cgacagcagg tctggggatt ggcatcgacc gtatggtaat gctgttcacc
    47041 aacagccata ccatccgcga cgttattctg ttcccggcga tgcgtccggt gaaataagca
    47101 ttacgttatg ctcacaaccc cggcaaatgt cggggttttt tatttaagct gggtaaatgc
    47161 agataatcgt attctggctt cgcgatttgt cgcctgcatc accatccacg gactgaacgc
    47221 ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa tcacaccatt gataatccat
    47281 cacttcatca tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata ccggacacac
    47341 ttcattttcc acaatgcctc tcggatcggt ggcgcggtag cgaaagtcag gatagataga
    47401 ttcaggaggc gtaatttcca cgccaagctc ataacggcaa cggcggatca ccgcgtcttc
    47461 gctgctttct cccagttgtg ggtgcccaca aaccgagtta gtccacacgc caggccacgc
    47521 ttttttgctc agtgcgcggc gggtaactaa taattgcccc ttggcattaa acagccaact
    47581 ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc
    47641 cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa tttctcacat
    47701 gtaattctga tcactcatca ttatagataa ttgaagagtg aaatgattaa ccaagagagc
    47761 gtttatcgac aaccgcccgt tttgagcgga tgccgacagg taaaaaccat ttaaaaaagt
    47821 agcatcatat atttgaagcg actttcaatg gccattgcaa cacagtcaga aatgacagga
    47881 atagagagag taaaaagcaa caacagactg aatatagcgc gcattaaaaa attcatatta
    47941 ttctccatgc tcatttttct tcagcttgcg catcagaagc cacatacgta ctgtcctgac
    48001 ttctaaatct ttatcagaga cggattcctt caccaggtca gcatgatgct gatagccatt
    48061 aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc tatgtagcag
    48121 cggttgcagt acggccggta acttagaaaa gaaatcgtga gagagcgtcg gagtcatgcc
    48181 aacgccgaga ctgatcgcca caatatagag gttgtaacgg ttggtggtgt agttacagcg
    48241 cgacagaatt cgaatccctg tcgccagtac catgccgaac atcactagac cagcgccgcc
    48301 cagcacaaat tgcggaatgg aggctaccag caccgccatt tttggcacca tgccaaacag
    48361 gattaatata attcccgaag aaatacacac ccagcggcta tgaacgcgca tcacgctaac
    48421 caggccaacg ttttgagaaa atgacgtgtg ggggaagctg ttaaacgtgc cgcctatcat
    48481 tgtccctacg ccatcgacac gcagcccgca aataatatcg tgtgaagaga gtttacgacc
    48541 gactatttca cccagtgcca ggaacatccc catcgactcg ataaacacga tgatt
//
LOCUS       DAJTNV010000021        48307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNV010000021 DAJTNV010000000
VERSION     DAJTNV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            180..473
                     /locus_tag="OO199_001198"
     CDS             180..473
                     /locus_tag="OO199_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5535311.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(514..1704)
                     /gene="nepI"
                     /locus_tag="OO199_001199"
     CDS             complement(514..1704)
                     /gene="nepI"
                     /locus_tag="OO199_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5535312.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1915..2367)
                     /locus_tag="OO199_001200"
     CDS             complement(1915..2367)
                     /locus_tag="OO199_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5535313.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2420..3754)
                     /gene="adeQ"
                     /locus_tag="OO199_001201"
                     /gene_synonym="yicO"
     CDS             complement(2420..3754)
                     /gene="adeQ"
                     /locus_tag="OO199_001201"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5535314.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3929..5695
                     /gene="adeD"
                     /locus_tag="OO199_001202"
     CDS             3929..5695
                     /gene="adeD"
                     /locus_tag="OO199_001202"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5535315.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5741..7132)
                     /gene="uhpT"
                     /locus_tag="OO199_001203"
     CDS             complement(5741..7132)
                     /gene="uhpT"
                     /locus_tag="OO199_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5535316.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7270..8589)
                     /gene="uhpC"
                     /locus_tag="OO199_001204"
     CDS             complement(7270..8589)
                     /gene="uhpC"
                     /locus_tag="OO199_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5535317.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8599..10101)
                     /gene="uhpB"
                     /locus_tag="OO199_001205"
     CDS             complement(8599..10101)
                     /gene="uhpB"
                     /locus_tag="OO199_001205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5535318.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10101..10691)
                     /gene="uhpA"
                     /locus_tag="OO199_001206"
     CDS             complement(10101..10691)
                     /gene="uhpA"
                     /locus_tag="OO199_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5535319.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10766..11056)
                     /gene="ilvN"
                     /locus_tag="OO199_001207"
     CDS             complement(10766..11056)
                     /gene="ilvN"
                     /locus_tag="OO199_001207"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5535320.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11060..12748)
                     /gene="ilvB"
                     /locus_tag="OO199_001208"
     CDS             complement(11060..12748)
                     /gene="ilvB"
                     /locus_tag="OO199_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5535321.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12854..12952)
                     /gene="ivbL"
                     /locus_tag="OO199_001209"
     CDS             complement(12854..12952)
                     /gene="ivbL"
                     /locus_tag="OO199_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5535322.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13517..13606
                     /gene="tisB"
                     /locus_tag="OO199_001210"
     CDS             13517..13606
                     /gene="tisB"
                     /locus_tag="OO199_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5535323.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13886..15070
                     /gene="emrD"
                     /locus_tag="OO199_001211"
     CDS             13886..15070
                     /gene="emrD"
                     /locus_tag="OO199_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5535324.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15078..15575)
                     /locus_tag="OO199_001212"
     CDS             complement(15078..15575)
                     /locus_tag="OO199_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5535325.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15572..15934)
                     /locus_tag="OO199_001213"
     CDS             complement(15572..15934)
                     /locus_tag="OO199_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5535326.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15924..16271)
                     /locus_tag="OO199_001214"
     CDS             complement(15924..16271)
                     /locus_tag="OO199_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5535327.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16380..16793
                     /locus_tag="OO199_001215"
     CDS             16380..16793
                     /locus_tag="OO199_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535328.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16840..18282)
                     /locus_tag="OO199_001216"
                     /pseudo
     CDS             complement(16840..18282)
                     /locus_tag="OO199_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18317..20032)
                     /locus_tag="OO199_001217"
     CDS             complement(18317..20032)
                     /locus_tag="OO199_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5535329.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20199..21092
                     /locus_tag="OO199_001218"
     CDS             20199..21092
                     /locus_tag="OO199_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5535330.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21089..21903)
                     /locus_tag="OO199_001219"
                     /pseudo
     CDS             complement(21089..21903)
                     /locus_tag="OO199_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21903..23520)
                     /locus_tag="OO199_001220"
                     /pseudo
     CDS             complement(21903..23520)
                     /locus_tag="OO199_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23815..24489
                     /locus_tag="OO199_001221"
     CDS             23815..24489
                     /locus_tag="OO199_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5535331.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24528..26189)
                     /locus_tag="OO199_001222"
     CDS             complement(24528..26189)
                     /locus_tag="OO199_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5535332.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26385..26813)
                     /gene="ibpB"
                     /locus_tag="OO199_001223"
     CDS             complement(26385..26813)
                     /gene="ibpB"
                     /locus_tag="OO199_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5535333.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26925..27338)
                     /gene="ibpA"
                     /locus_tag="OO199_001224"
     CDS             complement(26925..27338)
                     /gene="ibpA"
                     /locus_tag="OO199_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5535334.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27332..27404)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27643..27975
                     /locus_tag="OO199_001225"
     CDS             27643..27975
                     /locus_tag="OO199_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5535335.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27977..29197)
                     /locus_tag="OO199_001226"
     CDS             complement(27977..29197)
                     /locus_tag="OO199_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5535336.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29298..30398
                     /gene="cbrA"
                     /locus_tag="OO199_001227"
     CDS             29298..30398
                     /gene="cbrA"
                     /locus_tag="OO199_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5535337.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30359..31696)
                     /locus_tag="OO199_001228"
     CDS             complement(30359..31696)
                     /locus_tag="OO199_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5535338.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31771..32919)
                     /gene="dgoD"
                     /locus_tag="OO199_001229"
                     /pseudo
     CDS             complement(31771..32919)
                     /gene="dgoD"
                     /locus_tag="OO199_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32916..33533)
                     /gene="dgoA"
                     /locus_tag="OO199_001230"
     CDS             complement(32916..33533)
                     /gene="dgoA"
                     /locus_tag="OO199_001230"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5535339.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33517..34395)
                     /gene="dgoK"
                     /locus_tag="OO199_001231"
     CDS             complement(33517..34395)
                     /gene="dgoK"
                     /locus_tag="OO199_001231"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5535340.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34392..35081)
                     /gene="dgoR"
                     /locus_tag="OO199_001232"
     CDS             complement(34392..35081)
                     /gene="dgoR"
                     /locus_tag="OO199_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5535341.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35359..36015
                     /locus_tag="OO199_001233"
     CDS             35359..36015
                     /locus_tag="OO199_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535342.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMPFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36061..36873)
                     /gene="yidA"
                     /locus_tag="OO199_001234"
     CDS             complement(36061..36873)
                     /gene="yidA"
                     /locus_tag="OO199_001234"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5535343.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36987..37385)
                     /locus_tag="OO199_001235"
     CDS             complement(36987..37385)
                     /locus_tag="OO199_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5535344.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37625..40039)
                     /gene="gyrB"
                     /locus_tag="OO199_001236"
     CDS             complement(37625..40039)
                     /gene="gyrB"
                     /locus_tag="OO199_001236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5535345.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40068..41141)
                     /gene="recF"
                     /locus_tag="OO199_001237"
     CDS             complement(40068..41141)
                     /gene="recF"
                     /locus_tag="OO199_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5535346.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41141..42241)
                     /gene="dnaN"
                     /locus_tag="OO199_001238"
     CDS             complement(41141..42241)
                     /gene="dnaN"
                     /locus_tag="OO199_001238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5535347.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42246..43649)
                     /gene="dnaA"
                     /locus_tag="OO199_001239"
     CDS             complement(42246..43649)
                     /gene="dnaA"
                     /locus_tag="OO199_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5535348.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43946..44026
                     /locus_tag="OO199_001240"
     CDS             43946..44026
                     /locus_tag="OO199_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535349.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44256..44396
                     /gene="rpmH"
                     /locus_tag="OO199_001241"
     CDS             44256..44396
                     /gene="rpmH"
                     /locus_tag="OO199_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5535350.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44413..44772
                     /gene="rnpA"
                     /locus_tag="OO199_001242"
     CDS             44413..44772
                     /gene="rnpA"
                     /locus_tag="OO199_001242"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5535351.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44736..44993
                     /gene="yidD"
                     /locus_tag="OO199_001243"
     CDS             44736..44993
                     /gene="yidD"
                     /locus_tag="OO199_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5535352.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44996..46642
                     /gene="yidC"
                     /locus_tag="OO199_001244"
     CDS             44996..46642
                     /gene="yidC"
                     /locus_tag="OO199_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5535353.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46748..48112
                     /gene="mnmE"
                     /locus_tag="OO199_001245"
     CDS             46748..48112
                     /gene="mnmE"
                     /locus_tag="OO199_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5535354.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11991 a        13059 c        12203 g        11054 t
ORIGIN      
        1 ggtcgctgcg gtttaaaaaa cgaaacgcta tcgataagaa taaaaaggaa taaaagtgga
       61 atataaagtc tggcacttct tactgaccac gcaagcgcgt ttcgtacagc atgatgagag
      121 cgatgaaagc aaactacact tatgcttcat tcgatacaca tttgtaaaag gttgaatcca
      181 tgaaaccaac gacactactg cttattttca cttttttcgc tatgccaggc atcgtatacg
      241 ccgagtcgcc ttttagctca ctgcagtccg caaaagagaa aaccacggta ttacaagatt
      301 tacgtaaaat ttgcacgcca catgcgtcat tatcagatga agcgtgggaa aagttaatgt
      361 tgtcagatga aaacaataaa caacatatac gcgaggccat cgtggcgatg gaacgtaata
      421 atcagagcaa ttattgggaa gcgttgggta aggtagaatg cccggatatg taagaacagc
      481 aacaactact gaccaacgct tctttacctg atatcaggat ttcttcattt tcacctttgc
      541 agtaaccaac aatgcagtca gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag
      601 accaatatta tccagcgcat aaccgccgat tgccgcgcca caggtattag caagctgaat
      661 aaccgccacc tgaatagacc cggctttttc tgcctgatcg gccagcgagc gggtgatcca
      721 cgttgaccag ccgacgggaa ccaatgcaaa agttagcccc cagataatcg ccacgccggt
      781 agcaacgatt ttatcgcttc cccacaacgt cagtaccaac gcactcacag ccagtattaa
      841 cggcgcgcct gctaaggcca gttttaccga acgtttaaga atgaacgacg aaagcgacgt
      901 accaataaag ctggcgatac caaaactcaa cagcaccagc gttaagccat ccacgccgaa
      961 tcccgccagg ttcatataca ccgggcgaat atacgtgaag aaagcaaact gcccggcgaa
     1021 agacatgaag atggcgatca tccctgccat cacacccgga cgttgtaata agcggaaagt
     1081 attttgtttc tgatgcgagg gttcgcctgg cagtgaaggc aatgatttga taatccagaa
     1141 aatacacagc acgcccatca ccgccgccgc attaaagaca ttgcgccaac cgataagctc
     1201 gcctaaaaaa ctgcccaacg gcgcggcaat caccagcgca atagaaaccg cgccgaagat
     1261 caccgacagc gccttcggca ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga
     1321 catcgcccag aacccgccca gcgccagccc cagacaggca cgaccgatta aaagcaaact
     1381 gaatgagtta gcaaaggaaa ccagcaagca ggagagcgtc agcaaaacgg caaacaaaat
     1441 aacaacgtag cggcggtcag tggcctgaat tgtctgggta ataaacaaac tggcaaacat
     1501 tgccacaaag gcggtcacgg tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc
     1561 ctgggccatt ggcgtcaaca aactgacggg caaaaactca acgataatca gacaggcgac
     1621 acaaaacgcc accgagaaaa cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg
     1681 gttttcggca ataaattcac tcatggtgtt acccgtggca tgtttcaggg gaaaagccga
     1741 ccactttaac attgaaatgt gtgacgaatt taacgttttt gcaactttta cggtgttgct
     1801 tgagctggag ttaagatcga aacggaacag ggcaaagatt accggatgcg gcgtgaacgt
     1861 cttatccacc cgacaggtca ggcgcggtct gtaggctgat aagacgaaac ggtttcaatg
     1921 caaccagtga acgccctcgg caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt
     1981 aatatcctgc atttcaggtt cggtttcacg cagaaaaccc agcgccagcc tgacctgagc
     2041 atcggtaatc ggcgcggaaa ggcgggcttt ttgtaaaatc cggtgccatt gcagcaggtt
     2101 ctgttcgcta tcatcgacaa tgacaatttg ccagataagc aacacctgcg cggcgttttg
     2161 caggtgcgac tcatccgggc gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc
     2221 catttgatcg accatcgact ccacgggtac gacatcgata ttcagccgat cgctgagacg
     2281 gcgaatcgct tttctggccc cggatgtcag cacccgaaaa ccaaccacaa acactaccgc
     2341 cagcgtggcg agcattatcc agatcatgag ttctcctgcg taatcagaac tcatgatatc
     2401 tggaaaccct cgccagttac taatccacca gaataatctt caatgcgaac agagctgcga
     2461 ccaccaccac acacaggttc agatcgcgcc agcgcccggt gcacactttc atgatgcagt
     2521 acgacataaa gccgagtgca atcccttcgg tgatcgagaa agtaaagggc atcatcaccg
     2581 tggtaataaa cgcaggcacc gattcggtaa aatcatccca gttaacgcgc gccaggctcg
     2641 aagtcatcag cacgccaaca aagattaacg ctccagcggt tgcgtaagga ggaaccatcg
     2701 ccaccagcgg tgagaagaac ataactaaca ggaacataac gccgaccacg acagccgtta
     2761 gccccgtgcg accaccgact gccacaccag aagtactttc aatataggca gtaacagacg
     2821 aggtaccgat aaatgcgcct gccaccgaac tgacgctatc aacatacagc gccttattca
     2881 tattggggaa tttaccgttg ctatctatta agcccgcttt atcagttaca ccaattaatg
     2941 ttcctgatga atcaaatagg ttgatcagca taaaggagaa aatgataccg gcgagttcaa
     3001 gtgttaacgc gccgctcaaa tctacttcac caatgacgcc gctaatatca ggcggaatgg
     3061 aatagacgcc gctaaaatga acatcaccga aaaataatcc acagcaagac gtcaccacaa
     3121 tagaaaccag caccgcggca tgaaaatgac gtgatgacaa cacggttata ataaaaaacc
     3181 ctaaaatacc taacaacacg ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt
     3241 tattggcgac aataacacca gtgtttttta atcccattaa ggcaataaat aatccgattc
     3301 cactggtgat accaatacgt aaacttaatg gaatgttgga gatcatccag taccggatac
     3361 gaaacagcgt gagcaaaaat agcccaattg cgccccagaa tatcgcgccc atcccggtct
     3421 gccaggagat ccccatcgcc cccacgacca cgaacgcaaa gaaggcgttc agccccattg
     3481 ccggagccag cgccacgggt aagttagcaa atatccccat cgcaatactg ccgataccgg
     3541 caatcaaaca ggtggtaaca aacaccactt tcgggtccat ttgtgccgcg ccgaggattt
     3601 gcgggttcac aaaaacaatg tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt
     3661 ctgtgcggac ggtggtccca tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt
     3721 cattactcac gtaatcggta ttgtcattat tcattttttt gtccattgga ggagatttaa
     3781 tcccttgctt ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat
     3841 tgcacattaa tagatattct ttaataatct cgcgacgttt ctttatgata aataataatc
     3901 aaattgataa aatcaaaatg agaaaaatat gaataattcc attaaccata aatttcatca
     3961 cattagccgg gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga
     4021 ttatattatt gataatgtct ctattctcga cctgatcaat gccggagaaa tttccggccc
     4081 aattgtgatt aaaggacgtt acattgccgg tgttggcgca gaatacgctg atactccggc
     4141 tttgcagcgg attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct
     4201 gcatattgaa tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg
     4261 cctgacgacc gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt
     4321 cgcctggttt gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc
     4381 ttgcgtgccc gctctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat
     4441 gctcgcctgg cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg
     4501 cgtaattaac gggcagaatg cgctgctcga taaactcgat gcatttcgct acctgacgct
     4561 ggacggtcac tgtccgggtt tgggtggcaa agaacttaac gcctatattg ctgcgggtat
     4621 tgaaaactgc cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat
     4681 gtcgttgatg atccgcgaag ggtccgctgc cagcaatctc aacgcactgg caccgttgat
     4741 caacgaattt aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat
     4801 cgcccatgaa gggcatatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc
     4861 gctgcatgtg gcatatcgcg tcgccagctg gtcgacggca cgccactttg gtctgaatca
     4921 cctcggctta ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa
     4981 ggtcacggtg cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc
     5041 ggaagagtcg gcgagactgg cgctgtctgc cccgccatat ggcaatacca ttgcccgcca
     5101 gccagtttcc gccagcgact ttgccctgca attcacgccc ggaaaacgct atcgggtcat
     5161 tgacgtcatc cataacgaat tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg
     5221 ttttgatcgc gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc
     5281 tccggcctgt ggtttgctcg gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt
     5341 cagccatgac agccataata ttgtgatgat cggtcgcagt gccgaagaga tggcgctggc
     5401 ggtcaatcag gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca
     5461 aagtcatctg ccgttaccca ttgccgggct gatgagcacc gacacggcgc agtcgctggc
     5521 ggaacaaatt gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc
     5581 gtttattcag atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca
     5641 ggggctattt gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa
     5701 aaagcccggc gtcatgcagg gcaaaagtca ccagttacgt ttatgccact gtcaactgct
     5761 gaattttttt ctcgcggcgg attttgcgtt cttccattac cgccactatc gccatcaggc
     5821 agatacaacc aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc
     5881 cgaataccgg cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa
     5941 tcaggtaagc aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa
     6001 agccaacagc agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa
     6061 agagagaagc cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga
     6121 taatcagcgc cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga
     6181 gccagcccca cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa
     6241 tcgccaccgc tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga
     6301 taccaatacg taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt
     6361 ttttcagcac atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt
     6421 tgtcctcttc gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt
     6481 ccggggagtc gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg
     6541 ggaagataaa catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca
     6601 cacctgctgc gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc
     6661 cgcgtttacg acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac
     6721 tctggaaaaa gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc
     6781 tgcccatact ggcactgaag cccagcatac aaatggcaga gaggataagc atgaacggca
     6841 ggaattgttt ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat
     6901 aagtgatgga gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg
     6961 aaatcatatc gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc
     7021 cgataaagac caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt
     7081 cgagcggaag gtccagggtc ggcttgcgaa cctggtttag gaaagccagc atgggttact
     7141 cctgaaatga atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga
     7201 gatagcgtcc aagaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg
     7261 tgagatgcat cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc
     7321 ggaaatcccg gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag
     7381 cactttcgcc agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa
     7441 ccccgtcgcc gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa
     7501 catctgcggg ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta
     7561 gctggcaaat ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat
     7621 caaattcatc ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag
     7681 cgcaccgata aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc
     7741 gacgcccagc gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac
     7801 atagaccagc acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt
     7861 ggtgaggatc tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc
     7921 gtcgtgccgc cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg
     7981 ccagcagaga aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg
     8041 ccagccgtaa tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt
     8101 atgcgccgtg ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt
     8161 taacagacgc gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag
     8221 cacggcaaac gcccatagcg acgtcgagaa gccaaacaga atgttcatga tgcccgtggc
     8281 gataagccct atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac
     8341 aaacttcgac acgccgtaag taatgtaaaa cagggtcgct aacaggccga tatcgctacg
     8401 gctgagcacg ccgttagcaa ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa
     8461 gtaaaacagc gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata
     8521 acgggcatca atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa
     8581 cggcaacatc atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc
     8641 gtgcagacag gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt
     8701 gaggccaaaa ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac
     8761 cagcatcaac cgttcatcct gctgccagcc ttgcagcgta accgcgctgg catcggcatg
     8821 tttcacaatg ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc
     8881 gcttaatgct gattcatcga tacgccattc gagatggctg acaatgccgc gcccttccag
     8941 ctccatttcc cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg
     9001 cggacgtaac cgtcctaaca aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc
     9061 gatgagctgc ccgctctgct tcacactggc gttatctgcc gccagccgct gaacaatgcc
     9121 cgcctgagta cgaatagcag tgatggtctg accgatatcg tcgtgcagct cacgcgccac
     9181 gtcgcggcgc acgctctctt cggtttctaa caaacgttca gccagatgct gattgcgcgc
     9241 cagttccttt tgtagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa
     9301 caaccctgtc agactttgca ccagcaacga gagtaataaa tccaccagat gatcgcgcca
     9361 ggtttgactg gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca
     9421 gccatagtgc caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga
     9481 cagttcgtcc ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca
     9541 gaccagatgc cgcccgcgcc agttgatggg ctgagaaacc agtgacgggc cgagcggcag
     9601 ccaggtgtta ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcagacc
     9661 gccagttaaa gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag
     9721 cgactgcaac aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc
     9781 acgctgatgg cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat
     9841 catcaataac ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca
     9901 ctccgcaccc agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg
     9961 cagaccaaac ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat
    10021 actccatagg caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa
    10081 gcgggagaac aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg
    10141 ttactgacgc ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac
    10201 aagcccagtt cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc
    10261 tggcgttcac gtttggtcag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc
    10321 ggcgtcagat aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg
    10381 ctacagcgtt tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca
    10441 ggactgtcgt gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc
    10501 tccagaccgg agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc
    10561 ggcagccccg ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt
    10621 tccagcccca gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca
    10681 acggtgatca tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc
    10741 gcgatgttgt tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg
    10801 tcggatcgga ctgattacgc tgcactttca cgacatcttc cagcttagcg atttggctta
    10861 tcatctgctc cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt
    10921 cctgaatcgg cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac
    10981 aaacgtgggt cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgttat
    11041 gagttgtgtt ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca
    11101 ccatcgggta aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc
    11161 gattgatgat ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt
    11221 cgaggccgaa tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa
    11281 aaacgccttg ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt
    11341 tcatcagaat gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat
    11401 tcatcatcag gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg
    11461 ccgcgccaat cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc
    11521 actggcgtgg gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat
    11581 cggtggtgat aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat
    11641 ggcttaatgg atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta
    11701 ccaactggtg ccactctgca cgcggttgcg cttccacccg cgggatcaat tgcgccagca
    11761 cgtcatcaac atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc
    11821 gatcgatatc gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg
    11881 cccggtcatc aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt
    11941 tggtactgcg cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca
    12001 gcatgcccag cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac
    12061 gcacccgcgc aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag
    12121 cgttaatcat tgtagctgcg tcacgaatgc tttcttcgct aaaggcgggt gcagcggctt
    12181 tttctgccac cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa
    12241 tgtctatcca caccgggcta gggcggcctg attgcgcaat gcggaaggca tcgctcatga
    12301 cctgtgggag ctcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga
    12361 tgccgtaggt gtccacttct tggaaggcgt cggtgccgat catcgaggcg ggaacctgac
    12421 cagtgatgca aatcagcggg atggagtcca gccgcgcatc agcaatggcg gtcaccaggt
    12481 tagtcgcacc cggcccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca
    12541 ttccctgagc gataaacccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg
    12601 tgctttggct taaggcgtcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa
    12661 tcttaatgcc ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac
    12721 gcgtcgatgt tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc
    12781 gggaggtgct taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt
    12841 gttgttccag tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg
    12901 cggcggatgg cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc
    12961 ttgttggttt tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga
    13021 aaatttttca gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa
    13081 caaaaaaccc cgccggagcg aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac
    13141 actgtattat gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac
    13201 gaattttgac gtatttagtg catagttgag tatcgttcac agtttgcgtt ttgtccaaat
    13261 attactgttt atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg
    13321 gggccgcatc gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc
    13381 ttaaccccgc gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac
    13441 cgcgtgcaca acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga
    13501 accacaggag acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt
    13561 tgcagcactg caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc
    13621 gcaccaaagg ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagtcgg
    13681 atgcgatgct gacgcatctt atccgcccta ccatccctcc cggcaacatt tattgccgct
    13741 tttgtttaca tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat
    13801 tcatcaatgt gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat
    13861 tatttttata aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat
    13921 gttggtatta ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc
    13981 cgatatggcg cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta
    14041 tctgctgact tacggtgtct cacagctgtt ttatggcccg atatccgacc gtgtcggtcg
    14101 ccgaccagtg atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac
    14161 gacctccagt ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt
    14221 tggcggcgta atggcgcgta ctttgccgcg tgatttatat gaacggacac agttgcgcca
    14281 tgctaacagc ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg
    14341 cggtctgctg gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg
    14401 tgctggtgtg accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc
    14461 gcgcacgcgc ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta
    14521 tttgctgatg ctgattggcg gtctggccgg gattgctgcc tttgaagcct gctccggcgt
    14581 gctgatgggc gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc
    14641 gattccggcg gcattttttg gggcatggtt tgccgggcgt cctaataaac gcttctcaac
    14701 gttgatgtgg cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga
    14761 ctggtttggc gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg
    14821 tgccgggatg ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc
    14881 gggcacggct ggtgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc
    14941 gctctctgcg atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat
    15001 gggattgttg atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca
    15061 gcccgtttaa gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc
    15121 tttatcagat cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag
    15181 cccgcacaaa gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag
    15241 tcgcacgcct ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag
    15301 gtggcgtcaa aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcaaac
    15361 agggcaatta aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc
    15421 cggtcatcaa attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa
    15481 ttgtgataaa aatcggcagg ttgcagagca tccttgtact ctacaaccag cttatgcctg
    15541 tcctcttcag catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa
    15601 gttgatgtat atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca
    15661 aaaatttgtc acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat
    15721 tgcgattacg ggtgtagtgc cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa
    15781 tccagaataa catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc
    15841 cgtagcccag catggtacga aaccacgcca gtgatgtgcg ctccggctgc agccctgggt
    15901 cggcaatgcg tctggcttta cggctatccg gcatacaaca ccagtcccat aacgatcacc
    15961 accacaacca tcaatattaa gctgatgatt aataagctgt tggtgtaggg caggtcctct
    16021 ttcagacgca tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg
    16081 ccggaaaaca gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct
    16141 ggcgcaagtt gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac
    16201 gccagaaaag tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg
    16261 gaaattttca ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt
    16321 taagaatttc gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga
    16381 tgggtatcat agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt
    16441 taatgatggg gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg
    16501 aaagcatcgt ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag
    16561 gcgaaaaaat taccactgtg gagaaaaaag aggacatgga tatcgataaa atcctcgatc
    16621 aaagcggtat cgcactggcc attgctgcgc tactttgcgc atttattggc ggtatgcgta
    16681 aggaaaatcg ctggggcatt cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca
    16741 ttttgctgat attcctgatt ttttccttcc tgaccggcgg ttcgctggtt taagcagcct
    16801 gaataatgcc cgccgggtga tcatcagcgg gcattatcgt cagaatttct gttttttctc
    16861 ctccaccttc actccttgcg tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata
    16921 gccatcttgt gggcgtgggc gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca
    16981 cggacgcaga ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa
    17041 taaggcgtca tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg
    17101 catttcattt gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagcac
    17161 cagtttaaag tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg
    17221 ctcaatctcg tagcggttaa attccaccat cacgccgcgt ggctctttca cggcaaggat
    17281 attttccccc ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag
    17341 taaatcaata tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat
    17401 gatcagcgga atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg
    17461 tgcgcccatc atttcaccgt gatcggaggt ataaatgacc cacgtatttt cacgttgctc
    17521 tggcgttaag gcattgatga cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa
    17581 atagagcgga tggtgataaa gcccgtcatc accgactggc gatggcatcg cctgcgccca
    17641 taagcggtga tgttccggtt tgttcgccag gtcatcctgt gatttctcgc caagttcgta
    17701 gtaaaaatca gcgtatttct ctaaatactc caccggacag gtgaacgggt gatgcggctc
    17761 atcatacgaa accaccatca ggaagggttc ctcggcgcgc acgggctatt gcagaaaatc
    17821 taccgctcga ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg
    17881 taaatcttcg acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc
    17941 gctaagatag ttcgccccat caaaccagta atcagcatcc cactccggcg gacactcgcc
    18001 agtgccgaaa tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc
    18061 ggcatcctta aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt
    18121 ccacgggccg gactggttag cgtagatacc ggtgaatagt ccggcccgtg caggcgtaca
    18181 aaccggtgaa caagtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat
    18241 attttgcgta ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc
    18301 ggtcaattgg ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt acggctgggt
    18361 cgtgacgatg ccaccagctg tcaaaaatca ggtagacaat cactacggca gcgatgaaat
    18421 aactgatcat cgctaaccag cgcgtaccgt agccgccgaa ttcagccagc ccgacataga
    18481 cgccaatcat cgcgaacagg ataccaattg acgcgatctt gacgtttttc cacggtttca
    18541 tgtccaccgc aaacgcatct ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga
    18601 tcaccagcat cacgaccacg ttgatgcaga acgtacaggc cagcacatag aggaaatgga
    18661 agtcgaactt caccagatag ttgatggtga tgtagctgat tatgccaatc cccatcgcca
    18721 cttttgccgc cagcgccggg atgcgcggaa agaaaaagcc cataatgatg atggtaacca
    18781 gcggcacgtt gtaaatgccg ttgagctgtt tcatccagct atacagcccc tgcggcgcgt
    18841 tggcgatcca cggcgctacc aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc
    18901 cgacggtgac cagctgctgc ggctcggcat tctggttaat gatgcgacgg taaataccca
    18961 tactgaataa tgtactggcg ctattcagaa agccgttgaa ggtactgatc accgcaccaa
    19021 ataacaccgc gccgaagaaa ccctccagtg gcactggcaa aacgttattg accagcgtcg
    19081 ggtaggccat gtcggctttc ggtagatcct gatacagatg aaacgcaatc aaccctggca
    19141 gcaccagtac cagcgggtcg agcattttca gcaccgccgt taacagcgcc cccttttgcc
    19201 cttccgccag gctttttgac gccagcgtgc gttgcacgat gccctgattt gtacaccagt
    19261 aaaaggtgtt caccagaatt aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg
    19321 ggccaccgac tgagtttaat ttctcggcgt gaacggtggt aatttgctca atgccctgca
    19381 taaagctgcc cttgcctatc gcgatcaggc caaataccgg caccatcaac ccgccgataa
    19441 ccagcccaat accgttgatg gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg
    19501 catacaaaat tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag
    19561 aaatctgtag cgattccccg acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga
    19621 gaacaatcgg cagaaagcag acgccggtgg caatgaggaa gcagaagtcg atgataatac
    19681 gcgtcgtttt atcataacgt tcctccagaa aatcggggat ggtggcaatg ccgcgcttca
    19741 gatagcgcgg taaaaagatt agcgcgagga agatcagcgt caccgctgaa gtcacttccc
    19801 agcccatcac cgacatgccg cttttgtagg cctgcccgga aagaccaacc agttgttccg
    19861 tggaaaggtt ggttaacatt aacgaagcgg caataaccgg cgcttttagt gaacgtccgg
    19921 caagaaaata gccttgttgc gatccggtat ctgttttgcg aaccttccac caggtgatca
    19981 ccgccaccag cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc atctcatagc
    20041 ccttatttat gtttatgtcc cggacgcatg aatataggga gaggtaaaat gcgtcgggtc
    20101 aggcagtcag attcttaaaa tacaaacgtc gtatccctga acggattgtt ttctgttaag
    20161 ttcaggttgt gagcataatt cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt
    20221 cagatgtaaa aaacgaaact ccgtacaata ttcccttatt gattaacgaa aatgtgatct
    20281 ccagcggaat ttctctgatc tcgctctggc atacctacgc cgacgagcat taccgggtga
    20341 tctggccgcg ggacaagaag aaaccgctta tcgccaactc atgggttgcg gtgtataccg
    20401 tacaaggatg tgggaaaatt cttttaaaga atggcgaaca aataactctg catagcaact
    20461 gtattatatt tttaaagcca atggatattc actcttatca ctgtgaaggt ttagtctggg
    20521 aacagtactg gatggaattt acccccacca gtatgatgga tattcccgtt ggtcagcaaa
    20581 gcgttattta taatggcgaa atttataatc aggaactcac cgaagttgct gagttaataa
    20641 cttcaccaga agcaataaaa aataatctgg cagtcgcttt tctgacgaaa attatttatc
    20701 agtggatttg tcttatgtcc gcagatggta aaaaagatcc acaacggcgg caaattgaaa
    20761 aattaattgc cactttacat gccagtctgc aacaacgctg gagcgtagct gatatggctg
    20821 ccacgatccc ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat accggcaaga
    20881 cgccgaaaga atattacctc gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac
    20941 aaggaaactc ggttggcgaa gtcgctgata cgctcaactt cttcgactcc tttcatttca
    21001 gcaaagcctt taaacataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc
    21061 agcacccaac ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg
    21121 cctccagcaa ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca
    21181 acgtaatcgc ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt
    21241 tttccaccgc cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca
    21301 ccagtggttc aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat
    21361 ttgcgccatc cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt
    21421 cgggcgcaga cgacgcgtgg cttctgcgac aattgctgcc gggttggagt agttgagcat
    21481 ctaggcgttt ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga
    21541 acgcatgccg tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg
    21601 cagtgggatt ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat
    21661 gacaaaatca acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc
    21721 cggcgctttt tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc
    21781 atcgttatca taaaatttca gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat
    21841 caacacgatg cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt
    21901 cattatactg cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta
    21961 tcgagctgtt cacgcaattg ggatacatgc agaccaatga tcacctgaat ggcatcgcca
    22021 ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac
    22081 atgtcatgca gtgcaatacg tagacgcgtc gcgcaattgt taatgctgga gatattgccg
    22141 accccgccca gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgca
    22201 gcggtggttt ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca
    22261 tcttcacgtc ccggcgtgca catgttgaac tgcagaatca gggtgcggaa aaccacgaag
    22321 tacagcaggg taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg
    22381 ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg
    22441 cccaccacac caaagagata cattacggtc gacattgagg ccgccagcac ggcgtgtacc
    22501 gcaaacagca acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc
    22561 agcatggcgg ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt
    22621 gcggcagtga agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg
    22681 tgcagggcaa aaccgccttc cgggaacaac gatttcagtg gctcggcgct cagactgaac
    22741 tcttgcagat gctgcgccca gtacatctga atgccgcctt caacagctgc cggaccaaag
    22801 ataaacggtc cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag
    22861 gtgtaaaccc acaccccaag cgcacccgcc gaacgcagga acgcttgcag agattcaatc
    22921 cccatttgta cttttggcca gccgagcaat gtcagccagg cacaggggat catcaccagg
    22981 aaggcgataa tcaccacata agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt
    23041 ttatcgaaca gacggttatg cagcgccgtc acaatgccgg aaatgataat tgcgccgata
    23101 atgctggtat cgagggtttt aatcccggcc atcattgtca gaccgctacc tgccaccgcg
    23161 tcctgagtga aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc attgatgaaa
    23221 tagttccagg tcaggaaact caccatcacc gccagacaag cacgtccctg cgcttgctta
    23281 gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa
    23341 ccgccctctt caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat
    23401 tccccgacaa aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa
    23461 gggaaacagc agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat
    23521 ttccacatcc tcttttctca attctgtaag taggaagcct gattggttat gcggcagacc
    23581 tggagcgagg ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc
    23641 gctttcatgc ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg
    23701 tgagcacagg tacacagcat cgaacacatc tttaaaaaaa agattttttt caatcgatta
    23761 agcagaactt gtgggcgcat tacccgtgct tgcaggcaaa aaagagagct agagatgatc
    23821 tacaaaagca ttgcggagcg gttaagaatt cgacttaatt ccgcggattt cacgctaaac
    23881 agccttcttc ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgactatc
    23941 cgtaaagcca ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc
    24001 acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca gcctgaccgg actggtggag
    24061 gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct
    24121 gcgcctccgg ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc
    24181 cgtcgcgttc gttttgtgga agggaaaccg ctaatgctgg aagacagcta tatgccggta
    24241 aaactgttcc gtaacctttc gctgcaacat ctggaagggt cgaagtttga atatattgaa
    24301 caagagtgcg ggattttgat tggcggtaat tatgaaagcc tgatgccggt gctcgccgat
    24361 agactgctgg cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg
    24421 ttgtcatata gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc
    24481 gaatactagg tggagtacca tttacggcga ctccacccgg aaaaaagtta accgatactc
    24541 cagaagagca ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag
    24601 acagtggcgt aagagagcgc cgccgcacca ctggttggat gaagattatt agcaaacgcc
    24661 agcgccggag gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt
    24721 ttggctaaca tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca
    24781 ccataaccaa tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca
    24841 gatttcagac caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg
    24901 gcacttggcg gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac
    24961 gccataatca gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcctggcaca
    25021 aagacgggaa tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc
    25081 tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg
    25141 gacggacgcc ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg
    25201 accagttcga ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag
    25261 tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg
    25321 cgcaaatcag tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc
    25381 gcattatgta aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg
    25441 gtatctggcg aaggtacttt tagggtttct tctcgtttca gacgcgagca gataattttg
    25501 tcgccgttga gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg
    25561 cgaatattga tagtcttgat caacgcgccg ccattggtgc gtgaagactc gtgctgctga
    25621 gcttctgtct cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc
    25681 ccgcaaatgc cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc
    25741 attggtgtac ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtt
    25801 accgcaccgg agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg
    25861 atggcggtaa ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat
    25921 ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg
    25981 aacaggatca ggccaaactc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc
    26041 gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat
    26101 cctatgccgc gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga
    26161 atactgaccg ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt
    26221 gctacggatt atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg
    26281 aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga
    26341 caaaaaaagc cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg
    26401 ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt caggctcatt acgaattaaa
    26461 tcaatatgca gtaaaccgtt tacgaaggtt gcgccagaga cttccatatt ttcagccagc
    26521 gtaaaactca ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct
    26581 tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa
    26641 tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc
    26701 tcaatgttgt acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt
    26761 ttgtcaaaac cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt
    26821 tctccttcta agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac
    26881 ggcgagcatg gagatgtcag gccgcgccag gcggccttag ggaattagtt gatttcgata
    26941 cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt
    27001 accaggttag ccccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg
    27061 cgttcagcga tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct
    27121 ttcaccacca gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc
    27181 acagcgatag caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag
    27241 ccgccattac tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata
    27301 gcagaacggt aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag
    27361 cgagaatgta agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg
    27421 tcctcatcga ctacgatgta aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg
    27481 actttttttt gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa
    27541 gtgcgatagc cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat
    27601 aactcatcag gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg
    27661 ttagcgttca tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc
    27721 cataccgggg ggaaagaagg aacgtatccc ggcacccgcg ccagcgccac catgattggc
    27781 gacgatgaaa ccaactgggg cactaagtcg ctggcgattc tcgatatgcc gtttaccgct
    27841 gtaatggaca cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctcg
    27901 cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga
    27961 atgcctgata actgatttag cgatcagttt cggtgatcca cagctggcca ccttccatcc
    28021 acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt
    28081 ggttttcggt taaatactca acctcgccgc tttgcgcatg ggcacaaaca attcgattat
    28141 ccagcacaaa gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg
    28201 ttttgttatg ggttaacttg cgcggttcgc cgccctgtgg cgagataagc cacagttgca
    28261 caatgccgtt atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca
    28321 cccagtggcg cgggacgttg actaaccccg gataagcccg atgatgggta aaggttaaac
    28381 gtcgctgcac gacgccacgc ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg
    28441 catcacctgc cgatttccag ctagcttcat cttgcggtaa ctcaacgata aatagctccg
    28501 gcactttctc gccctttggc gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta
    28561 cccatccttc ttcataagca cgattgattt catcactgcc aggctgcggc gtgggcgtgg
    28621 ttttactcac cagcacacac cagtggctac cgctgtattc acgcggatgt tgcttttgta
    28681 cgttgaccgg gccaaacggt gcagcaaccc cgacgtttcg caaatccagc gccggatcga
    28741 gttgatgcat tacatggtcg ttataggtaa agctccccct ttcaccgttc gggctaaaga
    28801 catgcacatg gctgccaccg cgcagcgctc ctggcgtata cggcgcggta atatccattg
    28861 catcgagatt gctcaccttg ccgccttcag caatgactcc gcgtcgatga tggaagtcgt
    28921 aatgccatgt ttcatcagga ttttcagggc cgtgaataaa gacatatttc tctgacttcg
    28981 gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc gcgatagata acctcgacct
    29041 cgcctgtatg gatattcaca cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac
    29101 gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt attggtattg gtattggtga
    29161 gcaggtgatt acggggagca aaggttatct gtttcatggc gcggtcctga tacaaaacgg
    29221 ttcccgccat catacttcac aaggacttca ctttcagaca atagtctttt gccatcgtga
    29281 tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat tattggcctc ggcccggcag
    29341 ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt gatcgcgctg gataaaaagc
    29401 accagtgcgg tactgaaggt ttcagcaaac cttgcggcgg tctgctggca ccggacgcgc
    29461 agcgatcttt tattcgcgat ggactgacgc ttcctgtcga tgtgatcgcc aatccacaga
    29521 ttttcagcgt caaaactgtc gacgtcgccg catcgctcac gcgtaactac cagcgaagct
    29581 atatcaatat taatcgccat gctttcgact tgtggatgaa atcgctgatc cccgccagcg
    29641 ttgaggttta tcacgatagc ctgtgccgaa aaatctggcg tgaggatgat aaatggcatg
    29701 tcatttttcg tgcagacggc tgggagcaac atattaccgc ccgctatctg gtcggtgccg
    29761 atggcgcaaa ctcgatggtg cggcgacatc tctacccgga tcatcaaatc cgtaaatatg
    29821 tcgctatcca gcagtggttc gcagagaaac atccggtgcc gttctactcc tgcatctttg
    29881 ataatgcgat aactgactgt tactcatgga gtatcagcaa agatggttat tttatctttg
    29941 gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac gacgctgaaa gagaaaatga
    30001 gcgcctttca gttccagttt ggcaaagcgg tgaaaagtga gaaatgcacg gtgttgtttc
    30061 cctcacgctg gcaggatttt gtctgtggta aggacaacgc ctttctgatt ggtgaagcgg
    30121 cgggatttat cagcgccagc tcgctggagg ggattagcta tgcgctggat agcgcagaga
    30181 ttctgcgtgc ggtgttcctg aaacagccgg agaagagcaa cgccgcttac tggcgcgcca
    30241 cacacaaact gcgtttaaaa ctcttcggca agatagtaaa aagccgatgc ctgaccgcac
    30301 cggctttaag aaagtggatt atgcgcagtg gtgtggcgca tattccacag ttgaaagatt
    30361 atccaacgcg cttcacatca cccaccagca ggatgtaaga gagcgcgcca atcaacgcga
    30421 cggcggagat ataaaccagt gcaggcgcga aaccgtaacc ctgcgccagg taccccacca
    30481 ccagcggaac ggtgatgccg cccagaccac cggcgaagtt aaacacgccg ccggttaaac
    30541 caatcaggcg catcggtgcc agagaagaga ccagcgacca ggtaatcgaa gcaaaaccgt
    30601 tgccgaagaa tgccagcgcc atcaggcaca taatcatcat cggatcgttg gtgtagttag
    30661 cgcccataat gcaggtggag atcagcaagc cgcagataat cggcgtttta cgcgcaaagc
    30721 ccagtgaaaa gcccttacgt accagcagat ccgcgaccca gccagagagc aggacgccga
    30781 caaacgccgc gaggaatggc accgtggtca taaagcctgc tttcagcgcc gtgattcctt
    30841 tttcctgggt taaatagttc gggaaccagg ttaagaaaaa ccacagtgca gaagccaccg
    30901 caaattgccc aagataaacg ccgatcagtt tacgatggaa caccagtttc cagtctttgg
    30961 ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc accatccacc agaccgccgc
    31021 catcacgaat gtaatccagt tcagctttgc tgataccttt ggtcaggcgc ggcggctgat
    31081 aaaccttaaa ccaaatcagc gaccagataa tgccgatacc accagtgaca atgaacaccc
    31141 agtgccagct caacatctcc tgaatccaga tcagcagcgg agtcaaaaac gccagaccga
    31201 caaactgacc agacgtataa aaaccaacgg cggaagcgcg ttcatgttcc gggaaccagc
    31261 tggtcaccat ccggttattg gtcgggaaag cgggcgcttc gaaaataccg gttatcgcac
    31321 gcagaccaat taatgacatt aatcccgtgg caaagccttg gaataaagtc accactgacc
    31381 aaccaaatat cgcaataaaa taagtcacgc gagaacctac gcgatctaaa aaccaaccgc
    31441 cggggatctg gcatagcgta taaagccagg cgaaggccga aaatacatag cccatttccg
    31501 ctttcgtaat gccgaactct tcctgaatat gggcggaagc cacggccaag ttagcgcggt
    31561 caacataaca aatgaccacc gtaataaaga tcatcaccag cgtcagataa cgccgacgcc
    31621 ccggctttgc tgcattaacg ggaatatcca tagcgagctt tctccagatt ttgggcatag
    31681 cgaagccgct caccatgccc tgtaatttac agagggttat atttttgtat tgctgtttta
    31741 gtgcccgatg aggggcttac gtggcaggaa ttaccactct gctacgctgt tatcttcatg
    31801 acgccagagc ggattacgcc agtccggggc atttttactg aactcaataa ctttagcttc
    31861 gtcgatttcc acacctaagc ccggtttcgt taacggttta aagaagccgc caaccatgct
    31921 gaagtcttct ttgtttttca caaagtcgag taactccgcg cctttgttgt aatgaatgcc
    31981 catactttgt tactgaagta ccgcgttata ggaaacaaag tcgatatgca ggcaagccgc
    32041 cagtgcaatc ggtccgaacg gacagtgcgg cgcaagggtc acatcatagg cttctgccat
    32101 tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg gagagatccg gttgcagaat
    32161 cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa cgtgagaaca tgcgctcacc
    32221 cgccgccagt ggaatatgcg tttgtgccgc caatttcggg tagtattcgg cttgttccgc
    32281 cagcaccggc tcttcaataa acagcgggcg atacggctcc agttctttaa tcagcacttt
    32341 cgccattggc gcgctgacgc gaccgtggaa atcaagacca aactcaatct gattgccaaa
    32401 agcttcacga atttgtgcca cggtgttgac tgccgcatct accgcgcggg agttatcaat
    32461 tagccccagt tcttcacaac cgttcagttt gaaggtatcg aagccgattt cgcgcagggt
    32521 tttaatgccg tcgataacat ccgccggacg atcgccgccg acccaactgt aggctttaat
    32581 tttgtcgcga accaggccgc ccatcagttg ccagactggc gcattcagca ctttgccttt
    32641 gatatcccat aacgcctggt cgatcccggc gatggcgctc atcaggattg gaccgccacg
    32701 ataaaatccg gcgcgataca tcacttgcca taagtcgttg atgcgcgaag gatcctgacc
    32761 aatcaaatag tcacccagct cgtgaactgc cgcttccacc gtacgggcgc ggccttcaat
    32821 cacgggctcg ccccaaccga ccacgccttc atcggtttca attttcagga acatccagcg
    32881 gggaggtaaa cgatacgtgg taattttggt gattttcatt gcactgcctc tcgatacgcc
    32941 ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg actgcccggc gcgataaaga
    33001 tcgctgccta agcccgcccc aacacaacct gcgtctatcc actgcgccag gttttctggc
    33061 gtcacgccgc caacggcaaa gactgcgatg tccggtggta ataccgcttt taacgctttg
    33121 atgtattgcg gaccaaaagc cgatgacgga aatattttca gcgcctgcgc gcccgcttcg
    33181 agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc aaacggtcat gccgtagccc
    33241 accgcgcggc ggatcacttc actatggata ttgggcgtaa cgatgagctg acagcccatc
    33301 ctggcgagcg catcgacctg ttcaggtttc agtaccgtac ctgcgccaat caacgccttg
    33361 tcgccatacg catcaacgat ggcgggaatg ctctgctccc attgtgggga attcagcggg
    33421 atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg
    33481 ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag tttgccactg catgagcgat
    33541 gctccttata ccagcctgaa atgccgtgtc gcccgccacc gccgccacgt cgcaacccat
    33601 cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt ccggcgacaa gggtgatggc
    33661 gtgttgatgg gtcacatagt cgcgcatact ggcaacctct gcgccaatca acaaaccaga
    33721 gagaaattcg ctgacctgtt cgcggggaag tgttcccagc acatgcgagg cgcgaacttc
    33781 aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc tcaaggccag ccgcgaaggc
    33841 atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc aatgagtgat ttaacagtaa
    33901 atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg atttgctggc tatcggcctg
    33961 cacccatttg caatgcgtac cgggcataac ataaagagag gaaggagcca gagctcgcgc
    34021 gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg ttatcgtcat gagagacgca
    34081 taatccggga ataatccaga tattgtcgcc aacagacgtt aattgttcgc caatagacga
    34141 aaaacgggca ggaacagata aatacggagc aactttccag ccgacattgc tgccgaccat
    34201 tcctgccatc accactggcg tattttcttc acgccagtcg gtcgtgactt ctgctaacac
    34261 cacagccgga gattttccgt tcaggcgcgt gacgcctgct tctgattgcc tgctctccag
    34321 gcagtggtcg ccctgataaa gccaggcgcg cagattggtc gatccccagt caattgcgat
    34381 gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg agctggcaat catggtaagc
    34441 gccgcctgct ctgccgcatc gccgtcctga tgccgtatcg catcgaacag cgccttatgt
    34501 tcctggagcg tttgcggcat gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc
    34561 cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca ccgggttatg caccgactgc
    34621 agcaccgcct cgtggtagcg aatatccgct tcgttaaacg cttcgcggtc ctgattgttg
    34681 gcaatcatct cgttcagcgc cgattcaatc tgcgccagat cgctggaggt cgcgcgctct
    34741 gctgcccaac gggcaatcgc cggttccacc agatttcgca cttcgctcat ggcactgata
    34801 agccgtgggt cgtagtcgtt ttccagcacc cattgcagta cgtcagtgtc gaggtaattc
    34861 cactggttac gcggtgccac aaacgccccg cgataacgtt tcatttcaat cagccgcttc
    34921 gccatcagcg aacggaacac ctcacggatg atgttgcgcg aggttgcaaa ctcttcacag
    34981 agctccgcct cagccggaag tggcgagcca ggtacgtatt tgccgtgaac gatctgttta
    35041 cccagcgtaa tgacaatgcg atcggtttta ttgagagtca tggagagtcc ttgtgctctg
    35101 tatgttcttc tctactttac cccgatcgat gcataacgcg gcaactttgt agtaccagcg
    35161 tgatgacgtt cgcgtttgcc gcgcgcgtaa tgtagtacaa acttatattg ttatgctaca
    35221 atttagatca aaaaaagaac aatacataaa aaaatgacat gcgtcgggca gaaatctgaa
    35281 aagggatatc aggcgctaaa caggagggaa agaagagtat gctttcaacg gcttagctac
    35341 tcgtttaaag gattaatcat gaagttgaat tttaagggat tttttaaggc tgccggttta
    35401 ttcccactgg cgctgatgct ttcaggctgt atctcgtatg ctctggtttc ccataccgca
    35461 aagggtagtt caggaaagta tcaatcgcag tcagacacca tcactgggct atcgcaggca
    35521 aaagatagta atggaacaaa aggctatgtt tttgtagggg aatcgctgga ttaccttatc
    35581 actgatggtg ccgatgacat cgttaagatg ctcaatgatc cagcacttaa ccggcacaat
    35641 attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc
    35701 acaatatcgc tttactacta ctggaataac gaagaagaaa aggcactggc aacgcattat
    35761 ggttttgcct gtggtgttca acactgtacc aggtcactgg aaaacctaaa aggcacaatc
    35821 catgagaaaa ataaaaacat ggattactca aaggtgatgc cgttctacca tccatttaaa
    35881 gtgcgatttt atgaatacta ttcacccaga ggcattccgg atggtgtttc cgcagcatta
    35941 ctgccagtga ctgttacact ggacatcatt actgcaccgc tgcaatttct ggttgtatat
    36001 gcagtaaacc aataatcagt aagcgggcaa acgcgtttat gctgtttgcc cgcttacaga
    36061 ttaattcagc acatacttct caatagcaaa cgccacgcca tcttcaaggt tggattttgt
    36121 gacaaagttc gccacttctt tcaccgaagg aatagcgtta tccatcgcca caccgacgcc
    36181 tgcatattca atcattgcga tgtcgttttc ctgatcgcca atcgccatga tttcttccgg
    36241 tttaatacct aacacgtcgg ccagtgattt cacacccgta cctttgttaa cgcgtttatc
    36301 gaggatttcg aggaagtacg gcgcactttt cagcacggta tatttctctt tcacttcctg
    36361 cggaatacgc gcgatagcct ggtcgaggat ggcgggttca tcaatcatca tcactttcag
    36421 gaactgggta ttggggtcca ttttctccgc ttcgcagaac accagcggaa ttgtggcaac
    36481 gaaggattca tgcaccgtgt agtagctgat atcgcggtta gcggtgtaca gcgtggtgcg
    36541 gtccagggcg tggaaatgag aaccgacttc gcgagagagt ttttccagga aacgatagtc
    36601 gtcatagctg agagcggttt gcgccacggt gctaccatca gcggccttct gtaccagcgc
    36661 gccgttataa gtaatgcagt agtcgcccgg ctgttccata tgcagctctt tcaggtagtt
    36721 gtgcacacct gcatacggac gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc
    36781 tgcggcaatc gcatttttaa cggcgggtga aatggtgtga tcgggcagca gaagggtgcc
    36841 atccatatcg atagcaatga gtttgatagc catgagttcc caaggtagat tagttcctga
    36901 cccatgctaa cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa aggggatgca
    36961 acgcgggttg catgctcccc atttgcttag cggaagagtt tccctttcag cagttccatg
    37021 cctgcggaaa gcagatcgtt attggcttgt gctgacactt caccttgcgg tgagagcgca
    37081 tcaataatct tcggcaattg ctctgccagt aaactggaag ccgtactggt atccacgcca
    37141 agtttttgcc cgagatcgga caccgcattt gtgccgagcg ccgactccag ttgctcgccg
    37201 ctaactgatt gatttctctg ttgattactc aaccaggttg agagaatggc ccctaagccg
    37261 ccactttgca gtttttccag cagcacctga atgccaccct gctcctcaac ccaacttaaa
    37321 atcgcctgat atttccccgc atcgcctttc agaaaggcac cgacaacttc atcaaaaagc
    37381 cccatgataa tcacctgtaa agcgttacgt gttgacccaa aaagtataga tctgcgggat
    37441 gataattgcg gattgcagaa ataaaaaggg cggagatgat ctccgccctt ttcttatagc
    37501 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaatcatgca aaatcaatat
    37561 attgcaggag tctcgtagga ctaataagcg tagcgcatca ggcacgctcg catggttaac
    37621 gccattaaat atcaatattc gccgctttca gggcgttctc ttcaataaac gcacgacgcg
    37681 gttctacggc gtcgcccatc agcgtggtga acaactggtc agcggcaatc gcatccttaa
    37741 cggtaacgcg cagcatacga cggctttccg ggtccatggt ggtttcccac agctgttccg
    37801 ggttcatctc gcccagacct ttataacgct ggatggagag gccacgacgg gactctttca
    37861 ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg acgacgctcg ccacgttcga
    37921 taaacgcatc ttcttccagc aagccacgca gtttctcacc cagcgtgcag atacgacgat
    37981 attcgccacc ggtgataaac tcgtgatcca gcggatagtc agtatccaca ccgtgggtac
    38041 gcacgcgaac aatcggctcg aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc
    38101 actggctgcc gtgctgttct ttgtcgttca gttcgctgac cagcgcgttc acccagcggg
    38161 taacggtctg ctcatcagaa aggtcagctt ccgtcaacgt cggctgatag ataagctctt
    38221 tcagcattgc tttcggataa cgacgctcca tgcggttgat cattttctgc gtcgcgttgt
    38281 actcagatac cagtttctct aacgcttcgc cagccaatgc cggtgcactg gcgttggtgt
    38341 gcagcgttgc gccgtccagc gcgatagaga tctggtactg atccatcgct tcgtcgtctt
    38401 taatgtactg ttcctgcttg cctttcttca ctttgtacag cggcggctga gcgatgtaga
    38461 cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa gaaggtcaac agcagcgtac
    38521 gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga taacgcagtt
    38581 tgtccgggtt gtactcgtca cgaccgatac cacagccaag cgcggtgata agcgtcgcca
    38641 cttcctgaga agagagcatc ttatcgaagc gcgctttctc gacgttaagg attttaccct
    38701 tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg
    38761 agtccccttc caccaggtac agttcggaaa gcgccggatc gcgttcctgg cagtctgcca
    38821 gtttgcccgg caggccagct aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc
    38881 gacgcgccgc ttcacgggca cgggcagcat cgataatttt gccaaccacg attttcgcat
    38941 cagttgggtt ttccagcagg tattccgcca gcagttcgtt catctgctgt tcaaccgccg
    39001 atttcacctc agaagaaacc agtttgtctt tggtctggga ggagaatttc gggtccggca
    39061 cttttacgga aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt
    39121 tggctttttt gctgtagcct tctttgtcca tgtaggcatt cagggtacgg gtcatcgccg
    39181 cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt
    39241 agatgttttc ctggaagcca tcgttccact gcagcgccac ttcaacgcca ataccgtctt
    39301 tttcggtgga gaagtagaag atatttgggt ggatcggcgt tttgttcttg ttcagatatt
    39361 caacgaacgc tttgatgccg ccttcatagt ggaagtggtc ttctttgccg tcacgcttgt
    39421 cgcgcagacg aatggaaacg ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca
    39481 gaatttcata ttcgaactcg gtcacattgg tgaaggtttc gaggctgggc cagaaacgca
    39541 ccatggtgcc ggttttttca gtctcgccgg taaccgccag cggagcctgc ggtacaccgt
    39601 gttcgtagat ctgacggtga attttaccct cgcgctgaat aaccagctcc agtttttgcg
    39661 acagggcgtt tactaccgaa acaccaacgc cgtgcagacc gccggacact ttataggagt
    39721 tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc
    39781 cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt
    39841 tatcggcgtg aatggtgacg ataatttctt tacagtgacc cgcgagcgct tcgtcgatag
    39901 cgttatctac cacctcgaat accatgtggt gcagaccggt gccgtcatcc gtgtcgccga
    39961 tatacatacc cgggcgctta cgcaccgcat ccagcccttt caggactttg atactggagg
    40021 agtcataaga attcgacatc aacgtttctc gctcatttat acttgggtta atctgttatt
    40081 ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg acatgtctat aacgtgttca
    40141 gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct ggcaagcaac
    40201 ccgcgacgct catcatcaag ctcagaggca aaatcatcta tcaggtagag acaccgccgc
    40261 ccgctttcac gagtgaggaa ctctccttgc gccagacgta aggcgcacat caacagctta
    40321 agctgcccac gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc
    40381 gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc
    40441 agcacctcag cgtactctgt ctctttctcc cagccgcgct ggaaagagaa agtcagagaa
    40501 aactcaggga gaaattgctt acaggtatca tccatatcgg ccgcgatacc ggcgctatac
    40561 tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca gttctttatc ccacgggcgt
    40621 agctgttcgt aacgagtcac ctggcgcagc gccgcattgc gctgcttgag caatcgcttg
    40681 agattgctcc aggcggtgaa aaatccaggt tcgttgtgaa agcatcccca gtcgaggaat
    40741 gctcttctgt atttggggcc gccgttgagt aaagtaaacc cttctggcgt tatcaactgc
    40801 atcggcatca ggtgcgccag ttccgcgact ttatgcccgt ccgtaccgtc aatacggact
    40861 ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg tctcgcgctc ttcgccctgt
    40921 aatcgcccgt ggagaacaaa tgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa
    40981 ctgcgaaacg cccgaccatg gccgagcgta tagatggctt ccagcacgct ggttttgcca
    41041 ctgccgttgg cacctaccag aaagttaaag ccgggagata aagcgagatc cgcggtttca
    41101 atattgcgga aatcgcggat caacaagcgg gtgagggaca ttacagtctc attggcatga
    41161 caacataggc agccgactgt gatgcggcat cttcaatctg cacgctggaa accgaatcgg
    41221 tcagcatcat gcggacgttt tcgcatttca gcgcgttcag aacatccagc acatagctga
    41281 cgttgaagcc gatttccatc tccgcaccgc tataggtaac gtcgaggatc tcttccgctt
    41341 cttcctgttc cgggttgttg gcagtaattt tcagctggtt ttcgctgaca tacaggcgca
    41401 caccgcggaa tttctcgtta gagagaatcg ccgcgcgagc aaacgcctgc ttgagcagat
    41461 cgcagcctgc ttccagatgt ttgtccgggt tcttcggcag aacgcgacgg taatccggga
    41521 agcgaccatc caccagtttc gaggtgaaaa taaagtcgcc aacgtgggca cgaatgttat
    41581 tgctgccaat ctgcacacgc agcagattgt cgccgccgtc gagcatacgc atcagttcaa
    41641 tcacgccttt acgcggtacg atcaccgaat ggcttggcaa agattgacca attggcattg
    41701 aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg cagttcttca ccttcggttt
    41761 caaacagcat accgtttaag taatagcgaa catcctgatg cgccatcgaa aactgggttg
    41821 cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa ttcgacttcg ctctgccagt
    41881 catcgaggtt cgggaaatcc gccgctggca gggtagacag cgaaaaacgg ctacgcccgg
    41941 agcgcaccag catccgttca ccttccagct gcacggcaat ttccgcgcct tcaggcagac
    42001 cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct
    42061 gaaccagcgc aacgcgtgcc accatttcca tctcgagatc agtaccggtc agcgacaacg
    42121 taccgtcagc aacctgtaac agcagattac cgagaatcgg tagcgtagga cgaccaccta
    42181 acggaccgct cacctgttgt agcggtttta ataaatgctc acgttctacg gtaaatttca
    42241 taggtttacg atgacaatgt tctgattaaa tttgaaaaat cttctttgat atcgtggctc
    42301 tcttcacgca actgctcgat cttacggcag gcatgaagca ccgtcgtgtg gtcacgccca
    42361 ccaaacgcat cgccaatctc tggcagactg tggttagtca gctctttcgc tagcgccatc
    42421 gccatctggc gcggacgcgc caccgagcgg gatcgacgct tggaaaggag atccgcgact
    42481 ttgatcttgt agtactccgc caccgtcttc tgaatattgt cgatggtgac cagtttttcc
    42541 tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg
    42601 gtaaagttgg cgttagcaat aacgcggttc agcgcccctt ccagctcacg tacgttagat
    42661 cgtagacgct tggcgataaa gaacgccact tcacccggca aacgaatgtc attttcgtcg
    42721 gcctttttca tcaggatcgc cacacgggtt tccagctctg gcggttcgat cgccacagtc
    42781 agtccccaac cgaagcggga tttcaaacga tcctcaacgc cgttgatctc tttcggatag
    42841 cgatccgagg tgagaatgat ctgttgatta ccttccagca gggcgttgaa ggtgtggaaa
    42901 aactcttcct gagatcgttc tttattagca aaaaactgaa tatcgtcgat cagcagtgca
    42961 tctacggaac ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag ggctttaacc
    43021 atgtcctgaa caaagcgctc ggagtgcata taaaccactt tggcattcgg cttgcgcgcc
    43081 ataatgccgt tacccaccgc atgcagcagg tgagttttac ccagacccgt gccgccataa
    43141 aggaacaacg ggttataggc accgccaggg ttatccgcca cctggcaagc cgccgcgcgc
    43201 gccagttggt tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta
    43261 gaacgatagg tcggttctgc cggggccggg acgttatccc aacctgagcg cgtagaaggc
    43321 gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact
    43381 gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt caaaacgcag ctgtggggca
    43441 tccgctccgc agaaactggt tagcagtcca ttgatattat taaggtactt gtcccgtacc
    43501 caatcgagga caaaacggtt tggcgcgtac agggccagcg tgttatcgct cagttccgcc
    43561 tgcaatgggc gtatccacat actgaattct gtggctggta actcatcctg caatcgggca
    43621 agacactgct gccaaagcga aagtgacacg gcggactcca ctcgaacaaa agtcgataat
    43681 gactaaggct gaaacattca tgattgttga cgtacgtcga aaagaccctg cttgagggtg
    43741 acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac
    43801 cgcggatcat agcctaaact gcgcaagaga tcttctgttt ctcacagatt ttccctattt
    43861 atccacagga ctttccagaa ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga
    43921 tttacgccgc atattggaaa atttaatgac catagacaaa aattggctta atcgatctaa
    43981 taaagatcca ggacgatcct tgcgctttac ccatcagccc gtataatcct ccacccggcg
    44041 cgccatgctg gtttccactg gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa
    44101 agcgcggtaa ataaggaaag agaattgact ccggtgtgta caattattac aatccggcct
    44161 ctttaatcac ccatggcttc ggtgtccatc gtttcatttt tcggcggata tccaataaag
    44221 ccattgaatt tattcaagtt taggtagaaa tcgccatgaa acgcactttt caaccgtctg
    44281 tactgaagcg caaccgttct cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc
    44341 aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa
    44401 gctaacccct gagtggttaa gctcgcattt cccagggagt tacgcttgtt aactcccagt
    44461 caattcacat tcgtcttcca gcagccacaa cgggctggca cgccgcaaat taccattctc
    44521 ggccgcctga attcgctggg gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt
    44581 cgacgcgccc atgaacgcaa tcggattaaa cgcctgacgc gtgaaagctt ccgtctgcgc
    44641 caacatgaac tcccggctat ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc
    44701 gataaccgtg ctctctcgga agcgttggaa aaattatggc gccgccactg tcgcctggct
    44761 cgcgggtcct gatagccctc attcgggtct atcaacgcct gattagtccg ctactcgggc
    44821 cgcattgtcg tttcactcca acctgttcaa gctacggaat tgaggcattg cgcaggtttg
    44881 gagtgataaa aggcagttgg ttgacggtga aacgcgtatt aaaatgccac cctttacacc
    44941 ctggtggtga cgatcccgtc ccgcccggac catttgatac cagagaacac taacgatgga
    45001 ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg tctttcatga tctggcaagc
    45061 ctgggagcag gataaaaacc cgcaacctca ggcccaacag accacgcaga caacgaccac
    45121 cgcagcgggt agcgccgccg accagggcgt accggccagt ggccagggga aactgatctc
    45181 ggttaagacc gacgtgcttg atctgaccat caacacccgt ggtggtgatg ttgagcaagc
    45241 tctgctgcct gcttacccga aagagctgaa ctctacccag ccgttccagc tgttggaaac
    45301 ttcaccgcag tttatttatc aggcacagag cggtctgacc ggtcgtgatg gcccggataa
    45361 cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa gacgcttatg tgctggctga
    45421 aggtcaaaac gaactgcagg tgccgatgac gtataccgac gcggcaggca acacgtttac
    45481 caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa
    45541 cgctggcgag aaaccgctgg aaatctcctc gtttggtcag ttgaagcaat ccatcactct
    45601 gccaccgcat ctcgataccg gaagcagcaa cttcgcactg cacaccttcc gtggcgcggc
    45661 gtactccacg cctgacgaga agtatgagaa atacaagttc gataccattg ccgataacga
    45721 aaacctgaac atctcttcga aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac
    45781 ggcgtggatc ccgcataacg acggtaccaa caacttctat accgctaatc tgggtaacgg
    45841 catcgtcgct atcggctata aatctcagcc ggtactggtt cagcctggtc agactggcgc
    45901 gatgaacagc accctgtggg ttggcccgga aatccaggac aaaatggcag ctgttgctcc
    45961 gcacctggat ctgaccgttg attacggttg gttgtggttc atctctcagc cgctgttcaa
    46021 actgctgaaa tggatccata gctttgtggg taactggggc ttctccatta tcatcatcac
    46081 ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg cagtacacct ccatggcgaa
    46141 gatgcgtatg ctgcagccga agattcaggc aatgcgtgag cgtctgggcg atgacaaaca
    46201 gcgtatcagt caggaaatga tggcgctgta caaagctgag aaggttaacc cgctgggcgg
    46261 ctgcttcccg ctgctgatcc agatgccaat cttcctggcg ttgtactaca tgctgatggg
    46321 ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc cacgacctgt cggcacagga
    46381 cccgtactac atcctgccga tcctgatggg cgtaacgatg ttcttcattc agaagatgtc
    46441 gccgaccact gtgaccgacc cgatgcagca gaagatcatg acctttatgc cggtcatctt
    46501 caccgtgttc ttcctgtggt tcccgtcagg tctggtactg tactatatcg tcagcaacct
    46561 ggtaaccatt attcagcagc agctgattta ccgtggtctg gaaaaacgtg gcctgcatag
    46621 ccgcgagaag aaaaaatcct gattcggtga gttttcgcta aaataagggc ggtcagttga
    46681 ccgccttttt tcttttcgta gggcggataa gcaccgcgca tccgccacac aaagcaacag
    46741 gaacatcatg agcgataatg acactatcgt agcccaggcc acgcctccgg gacgtggcgg
    46801 cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa gttgccgaaa ccgtgctggg
    46861 taaactgcct aagccgcgct acgccgatta tcttccgttt aaagacgccg acggcagcgt
    46921 gctcgatcag gggattgcgc tatggttccc tggcccgaac tcgttcaccg gcgaagatgt
    46981 gctggaactg caaggtcatg gcggtccggt gatcctcgac ctgctgttaa aacgcattct
    47041 gaccattccc ggcctgcgga ttgctcgccc tggtgagttt tccgaacgcg cgtttcttaa
    47101 cgataaactt gacttagccc aggccgaggc gattgccgat cttatcgacg ccagttcgga
    47161 acaggcggcc cgttcggcac ttaactcgct gcaaggcgca ttctccgcac gggttaatca
    47221 tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa gcggcaattg atttccctga
    47281 tgaagagatc gatttcctct ccgacggcaa aattgaagcc cagcttaatg acgtgatggc
    47341 cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa
    47401 agtagtgatt gccggacgtc ctaatgccgg taaatcgagc ctgttaaacg cgctggcggg
    47461 ccgtgaagcg gcaatcgtaa ccgatatcgc cggaaccacg cgtgacgtgc tgcgtgagca
    47521 tatccacatt gacggaatgc cgctgcatat catcgacact gccgggctac gtgaggccag
    47581 tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag gaaattgaac aggccgaccg
    47641 cgtgctgttt atggtcgatg gcaccacgac agacgccgtt gatccggcag agatctggcc
    47701 ggaatttatc gcccgtctgc ccgcgaaact gccgatcacc gtggtacgca ataaagccga
    47761 tatcaccggc gaaacgctgg gaatgagtga agtgaacggt cacgcgttaa ttcgtctctc
    47821 ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat ctcaaacaga gcatgggctt
    47881 tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc cacttacagg cgctggaaca
    47941 ggcagcggag catttgcagc aaggtaaagc gcaactgttg ggagcctggg caggtgaact
    48001 gctggcggaa gagttgcgcc tggcacagca gaacttaagc gaaatcaccg gggaatttac
    48061 ttcagacgac ctgctggggc ggattttctc cagcttctgt attggtaagt aaccgcgctt
    48121 acgaagccgt attctgactg tcagatgcgg cttcgcttca ttgttagcac tcctgttatg
    48181 cctcaaccct tttttaaaca ttaaaattct tacgtaattt ataatcttta aaaaaagcat
    48241 ttaatactgc tccccgaacg attgtgattc gattcacatt taaacaattt cagaatagac
    48301 aaaaatt
//
LOCUS       DAJTNV010000022        47955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNV010000022 DAJTNV010000000
VERSION     DAJTNV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO199_001246"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO199_001246"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5535355.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO199_001247"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO199_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5535356.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO199_001248"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO199_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5535357.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMICRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO199_001249"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO199_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5535358.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO199_001250"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO199_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5535359.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO199_001251"
     CDS             5377..5835
                     /locus_tag="OO199_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5535360.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO199_001252"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO199_001252"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5535361.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO199_001253"
     tRNA            6887..6971
                     /locus_tag="OO199_001253"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO199_001254"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO199_001254"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO199_001255"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO199_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5535362.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO199_001256"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO199_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5535363.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO199_001257"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO199_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5535364.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO199_001258"
     CDS             complement(10252..13995)
                     /locus_tag="OO199_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5535365.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDRQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO199_001259"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO199_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5535366.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO199_001260"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO199_001260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5535367.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO199_001261"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO199_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5535368.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO199_001262"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO199_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5535369.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO199_001263"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO199_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5535370.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO199_001264"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO199_001264"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5535371.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO199_001265"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO199_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5535372.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO199_001266"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO199_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5535373.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO199_001267"
     CDS             23392..23499
                     /locus_tag="OO199_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5535374.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO199_001268"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO199_001268"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5535375.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO199_001269"
     CDS             complement(24538..25347)
                     /locus_tag="OO199_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5535376.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO199_001270"
     CDS             complement(25351..25743)
                     /locus_tag="OO199_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5535377.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO199_001271"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO199_001271"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5535378.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO199_001272"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO199_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5535379.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO199_001273"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO199_001273"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5535380.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO199_001274"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO199_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5535381.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO199_001275"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO199_001275"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5535382.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO199_001276"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO199_001276"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5535383.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO199_001277"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO199_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5535384.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO199_001278"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO199_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5535385.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO199_001279"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO199_001279"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5535386.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO199_001280"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO199_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5535387.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36789..39656
                     /locus_tag="OO199_001281"
     CDS             36789..39656
                     /locus_tag="OO199_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5535388.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="OO199_001282"
     CDS             complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="OO199_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5535389.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41903..42973
                     /gene="dhaK"
                     /locus_tag="OO199_001283"
     CDS             41903..42973
                     /gene="dhaK"
                     /locus_tag="OO199_001283"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5535390.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42984..43616
                     /gene="dhaL"
                     /locus_tag="OO199_001284"
     CDS             42984..43616
                     /gene="dhaL"
                     /locus_tag="OO199_001284"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5535391.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43627..45045
                     /gene="dhaM"
                     /locus_tag="OO199_001285"
     CDS             43627..45045
                     /gene="dhaM"
                     /locus_tag="OO199_001285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5535392.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45365..47062
                     /gene="treA"
                     /locus_tag="OO199_001286"
     CDS             45365..47062
                     /gene="treA"
                     /locus_tag="OO199_001286"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5535393.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47141..47580)
                     /locus_tag="OO199_001287"
                     /pseudo
     CDS             complement(47141..47580)
                     /locus_tag="OO199_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12035 a        12342 c        11748 g        11830 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcagatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccggtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg ttaaatatat attaattcct gtgttcctga ttttgcccgg taatagagaa
    36661 ttattctcag agaagctcat catgattgca ccatacagat atcatgatga taagaggata
    36721 ttattcattg acttaaacaa aatgacaaaa tccatagacc catagcctgg gtactatata
    36781 ggagtggtat gggcatcaaa caacacaatg ggaataccaa agccgatcgt ctcgctgaat
    36841 taaaaatccg ttcgccctca attcaactga taaaatttgg cgctattggt ttgaatgcaa
    36901 ttatcttttc ccccctgctg atagctgctg atacaggaag tcaatatggc accaatatta
    36961 ctattaatga tggtgacaga attacaggag ataccgccga tccatcagga aacctctatg
    37021 gtgtaatgac cccagcagga aacacgcctg gcaatatcaa cctgggtaat gatgtcaccg
    37081 tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat tattcagggc aaaaacagct
    37141 ccctgacagc taaccgactc acagtagatg ttgttggtca aacctctgcc atcggcatta
    37201 acttaattgg tgactatacc catgctgact taggcacagg cagcaccatt aagagtaacg
    37261 atgacggcat cattattggg catagctcaa cactaacagc cactcaattc accattgaaa
    37321 actcgaacgg tataggccta accatcaatg actatggcac cagtgtcgat cttggaagcg
    37381 gaagtaaaat caagaccgat ggaagtacag gtgtttatat cggtggtctc aacggcaata
    37441 acgccaatgg tgctgcgcgt tttacggcga cagacctgac aatcgatgtt cagggctaca
    37501 gcgccatggg gataaacgta cagaaaaact ctgttgtcga tctcggaaca aacagtacca
    37561 ttaaaaccaa tggcgataat gctcacggcc tctggagctt tggccaggtt agcgcgaatg
    37621 cactcactgt tgatgtaact ggagccgcgg ccaatggcgt cgaagttcgt ggtggtacaa
    37681 ccactatcgg tgcagatagc catatttctt ccgcgcaggg cggtggcctc gtcaccagta
    37741 gttcagacgc gacaatcaat ttttctggca cggcagcgca acgaaacagc atcttttccg
    37801 gcggttctta tggtgcctcg gcccagacgg caacggctgt tatcaacatg caaaataccg
    37861 atattacggt tgatcgtaat ggcagtctgg cgctgggttt gtgggcgctc agcggcggta
    37921 gaataaccgg agacagtttg gctatcaccg gcgcggcagg agccagaggg atttatgcca
    37981 tgaccaacag ccagatcgac ctcacgagcg atctggtcat tgatatgagt acacccgacc
    38041 agatggccat cgcaacgcaa catgacgatg gttatgccgc cagccgcatc aacgcctcgg
    38101 gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg gctaatcaat ctggatatgc
    38161 accctgggtc ggtttggaca ggttcctccc tcagcgataa tgtcaatggc gggaaactgg
    38221 acgttgcaat gaataacagc gtctggaacg taacaagtaa ttctaatctc gacacgctgg
    38281 cgctgagcca ttcaactgtc gattttgcca gccacgggtc aactgccggc acatttacca
    38341 cattaaacgt agagaacctg agcggtaaca gtacctttat tatgcgtgct gatgttgttg
    38401 gcgagggtaa tggcgttaat aatagagggg atttattgaa tatcagcggg agtagtgctg
    38461 gtaatcacgt attggctatc cgcaaccagg gcagcgaggc cacaacggga aatgaagttc
    38521 tgacagtggt aaaaaccact gacggcgcgg cctcgttcag cgcgtcttct caggttgagt
    38581 tggggggata tctgtacgat gtgcgtaaaa atggcactaa ctgggagctt tacgcttccg
    38641 ggacagttcc ggaaccgact cctaatcctg aacccacacc agctcccgct cagcctccca
    38701 tagtcaaccc cgatcctacg cctgaacccg ctcccacgcc taaacccacc acgaccgcag
    38761 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
    38821 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
    38881 gttatggggg aagcctggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
    38941 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
    39001 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
    39061 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
    39121 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaacg
    39181 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
    39241 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
    39301 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
    39361 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
    39421 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
    39481 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
    39541 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
    39601 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
    39661 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
    39721 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
    39781 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
    39841 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    39901 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
    39961 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    40021 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
    40081 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    40141 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    40201 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    40261 accagcattg cgaggtccac ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
    40321 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
    40381 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
    40441 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
    40501 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
    40561 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    40621 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    40681 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    40741 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    40801 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    40861 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    40921 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
    40981 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    41041 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    41101 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
    41161 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    41221 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    41281 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    41341 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
    41401 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
    41461 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    41521 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
    41581 catgtctccc gtttcatcag cttattgcat cgctcccagg aggttgcaat taagggagat
    41641 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
    41701 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    41761 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    41821 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    41881 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    41941 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
    42001 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
    42061 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
    42121 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
    42181 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    42241 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    42301 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
    42361 agtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggaggcctg
    42421 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    42481 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
    42541 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
    42601 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
    42661 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
    42721 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
    42781 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
    42841 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    42901 gaaagttgat gacgaaacgc tggctctctg ggacgccccg gtccacaccc cggcccttaa
    42961 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    43021 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
    43081 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    43141 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
    43201 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
    43261 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
    43321 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    43381 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
    43441 tggcgctcga agctgccagt agcatcgccg aatccgctgc acaaagtacg attacgatgc
    43501 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
    43561 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    43621 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
    43681 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
    43741 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    43801 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
    43861 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    43921 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
    43981 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
    44041 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
    44101 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    44161 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
    44221 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    44281 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcacctggc tgaagataac
    44341 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
    44401 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
    44461 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
    44521 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    44581 catacactgt taggtgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    44641 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    44701 caactggatg atgaatatct acaagctcgc tatattgatg tggacgatct tctgcatcgc
    44761 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt
    44821 ctactggcgg agaacattta tccttccaca gtactgcaac tggatccggc ggttgtaaaa
    44881 ggtatctgcc ttagcgccgg aagtccggta tcccacagcg ccctaatcgc ccgtgaactg
    44941 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    45001 ctaacgctgg acgttaaaac gcaacgtttc aaccgtcagg gttaagccac cgttcggatg
    45061 gcatcgttct gatgtcatcc ggcaatttac ccagcactga tgttttttaa ctacctgtta
    45121 tattttttac caatttatga tgatttttct tcgctaatcg cgaaccaaat gtcgctggct
    45181 ggctaataaa cagacgttat ttgatcactc tatttcgccg ttatttatcc ctttaattcc
    45241 cttttctaaa atgcctgacc gttcgcagaa tgagatttcg atcatgcagc tagtgcgatc
    45301 ctgaactaag gttttctgat acttgaatac cgttttattc ggtttcgcca aaggagaatg
    45361 attgatgaaa tcccccgcac cttctcgccc gcaaaaaatg gcgttaattc cagcctgtat
    45421 ctttttgtgt ttcgctgcgc tatcggtgca ggcagaagaa acaccggtaa caccacagcc
    45481 gcctgatatt ttattagggc cgctgtttaa tgatgtgcaa aacgccaaac tttttccgga
    45541 ccaaaaaacc tttgccgatg ccgtgccgaa cagcgatccg ctgatgatcc ttgctgatta
    45601 tcggatgcag caaaaccaga gcggatttga tctgcgccat ttcgttaacg tcaatttcac
    45661 cctgccgaaa gaaggcgaga aatatgttcc gccagagggg cagtcactgc gcgaacatat
    45721 tgacggactt tggccggtat taacgcgttc taccgaaaac accgaaaaat gggattctct
    45781 gttaccgctg ccggaacctt atgtcgtgcc gggcggacgt tttcgcgagg tatattactg
    45841 ggacagttac ttcaccatgt taggacttgc cgaaagtggt cactgggata aagtcgcgga
    45901 tatggtggcc aattttgctc atgaaataaa cacttacggt catattccca acggcaaccg
    45961 cagttactat ttaagccgct cgcaaccgcc cttctttgcc ctgatggtag agttactggc
    46021 gcagcatgaa ggcgatgccg cgttgaagca atacctgccg caaatgcaaa aagaatatgc
    46081 ttactggatg gacggtgttg aaaacctgca agccggacaa caggaaaaac gcgttgtaaa
    46141 acttcaggat ggtacccttc tcaaccgcta ctgggacgat cgcgatacgc cacgaccaga
    46201 gtcatgggtg gaagatattg ccaccgccaa aagcaatccg aatcgacctg ccactgaaat
    46261 ttaccgcgac ctgcgctctg ccgctgcgtc tggctgggat ttcagctcgc gctggatgga
    46321 caacccgcag cagttaaata ccttacgcac caccagcatc gtaccggtcg atctgaacag
    46381 cctgatgttt aaaatggaaa aaatcctcgc ccgcgccagc aaagctgcag gagataacgc
    46441 gatggcaaac cagtacgaaa cgctggcgaa tgcccgtcaa aaagggatcg aaaaatacct
    46501 gtggaacgat caacaaggct ggtatgccga ttacgacctg aaaagtcata aagtgcgcaa
    46561 tcagttaacc gcggccgccc tgttcccgct gtacgtcaat gcggcagcga aagatcgcgc
    46621 caacaaaatg gcgacggcga cgaaaacaca tctgctgcaa cccggcggcc tgaacaccac
    46681 gtcggtgaaa agtgggcaac aatgggatgc gccaaatggc tgggcaccgt tacagtgggt
    46741 cgcgacagaa ggattacaaa actacgggca aaaagaggtg gcgatggaca ttagctggca
    46801 cttcctgacc aatgttcagc acacctatga ccgggagaaa aagctggtgg aaaaatatga
    46861 tgtcagcacc accggaacgg ggggcggcgg tggcgaatat ccattacagg atggctttgg
    46921 ctggaccaat ggcgtgacgc tgaaaatgct ggatttgatc tgcccgaaag agcaaccgtg
    46981 tgacaatgtt ccggcgacgc atccgaccgt taagtcagca acgacgcaac cctcaaccaa
    47041 agaggcacaa cccacacctt aaccagcgct tactccgaca acgtcattcg gcttctccgg
    47101 atggcgttgt cggcagataa caccatatct atggtttttt ctacacagac ggtttttcaa
    47161 gtcctggcgg gaagtattgc gcaaatgttt gccatacgtg gttgccgaag atagcgtcat
    47221 ttctacgggt aagtgtttca ctttcatcac ccggcgattt acgccgacgt tggctatata
    47281 aagcatcaat tgctaccgaa tattgttacg gtgttcactc atcaacgttt ttttgctttc
    47341 tggagttaaa ttaataatca cataatccag acactccaga gaatttagca accaaaaaaa
    47401 gaattcagac cattcctctt cgcgtaactg aagcattgcc gaacgggtat tttggcttaa
    47461 tctgccagaa ggattgatca attctaaatg ttcaataagc gcagccaaaa ccgcctcact
    47521 atccgcccca tttttaacgc ctgactggct actgtctaaa atatttattt tctgaatcac
    47581 accaaaaaca acgaaacaat aattaaaaaa ggattctgtt gttgttaccg ggaaccgact
    47641 tatttcgctc agaaagtcgt tcaaccagct ccgcagtgat aagattcgct gatgatacag
    47701 tgtatcctca ctgcgtgctg gaggtgccag tataatatgt tgatcaatac agtatttatt
    47761 gtgttcagtc gtttcctgat gcgtttggtt ctctgcggat tgtcgctggc gaaataaatg
    47821 gcttgcggta aatgccgtta cagagcagcg caacgcgtca attccttcgg agtgcccggc
    47881 ttttggcgag agttccggtt ttatgcgtcc aggtagcgga aatttcaccc gaatgagaaa
    47941 tgcattaata aaatg
//
LOCUS       DAJTNV010000023        47329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNV010000023 DAJTNV010000000
VERSION     DAJTNV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..482
                     /gene="sseA"
                     /locus_tag="OO199_001288"
     CDS             <1..482
                     /gene="sseA"
                     /locus_tag="OO199_001288"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5535394.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1303..2079)
                     /gene="sseB"
                     /locus_tag="OO199_001289"
     CDS             complement(1303..2079)
                     /gene="sseB"
                     /locus_tag="OO199_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5535395.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2221..3504)
                     /gene="pepB"
                     /locus_tag="OO199_001290"
     CDS             complement(2221..3504)
                     /gene="pepB"
                     /locus_tag="OO199_001290"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5535396.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3563..3763)
                     /gene="iscX"
                     /locus_tag="OO199_001291"
     CDS             complement(3563..3763)
                     /gene="iscX"
                     /locus_tag="OO199_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5535397.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3775..4110)
                     /gene="fdx"
                     /locus_tag="OO199_001292"
     CDS             complement(3775..4110)
                     /gene="fdx"
                     /locus_tag="OO199_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5535398.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4112..5962)
                     /gene="hscA"
                     /locus_tag="OO199_001293"
     CDS             complement(4112..5962)
                     /gene="hscA"
                     /locus_tag="OO199_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5535399.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5979..6494)
                     /gene="hscB"
                     /locus_tag="OO199_001294"
     CDS             complement(5979..6494)
                     /gene="hscB"
                     /locus_tag="OO199_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5535400.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6590..6913)
                     /gene="iscA"
                     /locus_tag="OO199_001295"
     CDS             complement(6590..6913)
                     /gene="iscA"
                     /locus_tag="OO199_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5535401.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6930..7316)
                     /gene="iscU"
                     /locus_tag="OO199_001296"
     CDS             complement(6930..7316)
                     /gene="iscU"
                     /locus_tag="OO199_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5535402.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7344..8558)
                     /gene="iscS"
                     /locus_tag="OO199_001297"
     CDS             complement(7344..8558)
                     /gene="iscS"
                     /locus_tag="OO199_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5535403.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8670..9158)
                     /gene="iscR"
                     /locus_tag="OO199_001298"
     CDS             complement(8670..9158)
                     /gene="iscR"
                     /locus_tag="OO199_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5535404.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9428..10168)
                     /gene="trmJ"
                     /locus_tag="OO199_001299"
     CDS             complement(9428..10168)
                     /gene="trmJ"
                     /locus_tag="OO199_001299"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5535405.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10287..11090
                     /gene="suhB"
                     /locus_tag="OO199_001300"
     CDS             10287..11090
                     /gene="suhB"
                     /locus_tag="OO199_001300"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5535406.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11235..12089
                     /locus_tag="OO199_001301"
     CDS             11235..12089
                     /locus_tag="OO199_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5535407.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12280..13560
                     /gene="csiE"
                     /locus_tag="OO199_001302"
     CDS             12280..13560
                     /gene="csiE"
                     /locus_tag="OO199_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5535408.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13552..14691)
                     /locus_tag="OO199_001303"
     CDS             complement(13552..14691)
                     /locus_tag="OO199_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5535409.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14851..15741)
                     /gene="hcaR"
                     /locus_tag="OO199_001304"
     CDS             complement(14851..15741)
                     /gene="hcaR"
                     /locus_tag="OO199_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5535410.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15877..17238
                     /locus_tag="OO199_001305"
     CDS             15877..17238
                     /locus_tag="OO199_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5535411.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17235..17753
                     /locus_tag="OO199_001306"
     CDS             17235..17753
                     /locus_tag="OO199_001306"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5535412.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17753..18073
                     /locus_tag="OO199_001307"
     CDS             17753..18073
                     /locus_tag="OO199_001307"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5535413.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18070..18882
                     /gene="hcaB"
                     /locus_tag="OO199_001308"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18070..18882
                     /gene="hcaB"
                     /locus_tag="OO199_001308"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5535414.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18892..20094
                     /locus_tag="OO199_001309"
     CDS             18892..20094
                     /locus_tag="OO199_001309"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5535415.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20191..20613
                     /locus_tag="OO199_001310"
     CDS             20191..20613
                     /locus_tag="OO199_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5535416.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20661..21533)
                     /locus_tag="OO199_001311"
     CDS             complement(20661..21533)
                     /locus_tag="OO199_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5535417.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21545..22639)
                     /locus_tag="OO199_001312"
     CDS             complement(21545..22639)
                     /locus_tag="OO199_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5535418.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22672..23670)
                     /locus_tag="OO199_001313"
     CDS             complement(22672..23670)
                     /locus_tag="OO199_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5535419.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23695..25206)
                     /locus_tag="OO199_001314"
     CDS             complement(23695..25206)
                     /locus_tag="OO199_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5535420.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25229..26212)
                     /locus_tag="OO199_001315"
     CDS             complement(25229..26212)
                     /locus_tag="OO199_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5535421.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26309..29410)
                     /locus_tag="OO199_001316"
     CDS             complement(26309..29410)
                     /locus_tag="OO199_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5535422.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29417..29590)
                     /locus_tag="OO199_001317"
     CDS             complement(29417..29590)
                     /locus_tag="OO199_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535423.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29708..30901
                     /locus_tag="OO199_001318"
     CDS             29708..30901
                     /locus_tag="OO199_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5535424.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVIKVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31099..32352)
                     /gene="glyA"
                     /locus_tag="OO199_001319"
     CDS             complement(31099..32352)
                     /gene="glyA"
                     /locus_tag="OO199_001319"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5535425.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32679..33869
                     /gene="hmpA"
                     /locus_tag="OO199_001320"
     CDS             32679..33869
                     /gene="hmpA"
                     /locus_tag="OO199_001320"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5535426.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33914..34252)
                     /gene="glnB"
                     /locus_tag="OO199_001321"
     CDS             complement(33914..34252)
                     /gene="glnB"
                     /locus_tag="OO199_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5535427.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34313..35647)
                     /gene="glrR"
                     /locus_tag="OO199_001322"
     CDS             complement(34313..35647)
                     /gene="glrR"
                     /locus_tag="OO199_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5535428.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35637..36350)
                     /gene="qseG"
                     /locus_tag="OO199_001323"
     CDS             complement(35637..36350)
                     /gene="qseG"
                     /locus_tag="OO199_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5535429.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36515..37942)
                     /gene="qseE"
                     /locus_tag="OO199_001324"
     CDS             complement(36515..37942)
                     /gene="qseE"
                     /locus_tag="OO199_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5535430.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLVKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38518..42405)
                     /gene="purL"
                     /locus_tag="OO199_001325"
                     /gene_synonym="purI"
     CDS             complement(38518..42405)
                     /gene="purL"
                     /locus_tag="OO199_001325"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5535431.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42664..44220
                     /gene="mltF"
                     /locus_tag="OO199_001326"
     CDS             42664..44220
                     /gene="mltF"
                     /locus_tag="OO199_001326"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5535432.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44217..44753)
                     /gene="tadA"
                     /locus_tag="OO199_001327"
     CDS             complement(44217..44753)
                     /gene="tadA"
                     /locus_tag="OO199_001327"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5535433.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44778..45413)
                     /gene="yfhb"
                     /locus_tag="OO199_001328"
     CDS             complement(44778..45413)
                     /gene="yfhb"
                     /locus_tag="OO199_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5535434.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45622..46470
                     /locus_tag="OO199_001329"
     CDS             45622..46470
                     /locus_tag="OO199_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5535435.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46809..47329)
                     /locus_tag="OO199_001330"
                     /pseudo
     CDS             complement(46809..47329)
                     /locus_tag="OO199_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11288 a        12960 c        11899 g        11182 t
ORIGIN      
        1 gacttgcagg ctggcagcgc gatgacctgc tattagaaga aggtgcagta gagctgccgg
       61 aaggagagtt taacgccgcg tttaatcctg aagcagtggt gaaagtaacc gatgtgttat
      121 tggcaagcca tgaaaatacg gcgcaaatta ttgatgcccg cccggctgca cgttttaacg
      181 cagaagttga tgaacctcgc ccaggtttac gtcgcggaca tattcccggt gcattgaacg
      241 ttccgtggac ggaactggtg cgcgaaggcg aactaaaaac gaccgatgaa ctggatgcga
      301 tattttttgg tcgcggcgtc agctatgaca aaccgattat cgtcagctgc ggctctggtg
      361 taacggcagc cgtggttttg ttagcactcg cgacgctgga tgtgccaaac gtgaaactgt
      421 acgacggcgc atggagtgaa tggggcgcgc gggcagattt accggttgag ccagtgaaat
      481 aagtatttta caggcaataa aaaaccgccg aatttggcgt ttttttattg ctggtctggt
      541 tcgcggcctt tccagcaggt tgacttgtgt tacacatgag caacgcaggt gcttcacagc
      601 aaaacaatac tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat
      661 ttagcgtatt atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta
      721 ttagtccgaa ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt
      781 aaaagatatt acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa
      841 tcaggcggtt atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta
      901 gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca
      961 aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg
     1021 caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag
     1081 cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc
     1141 gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc
     1201 aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga
     1261 gcagcaagta agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg
     1321 tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt
     1381 aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc
     1441 gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc
     1501 atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca
     1561 aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat
     1621 ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc
     1681 ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa
     1741 ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa
     1801 caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc
     1861 ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa
     1921 atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac
     1981 ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt
     2041 ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac
     2101 gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt
     2161 gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt
     2221 ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca
     2281 ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg
     2341 atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc
     2401 cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc
     2461 cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc
     2521 ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc
     2581 cgccccggtg agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat
     2641 cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt
     2701 accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc
     2761 cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag
     2821 cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt
     2881 ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta
     2941 caccggtgct tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg
     3001 ttctgaaccg cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc
     3061 tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg
     3121 ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca
     3181 gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca
     3241 ctccacttta cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc
     3301 cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg
     3361 ggccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc
     3421 attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag
     3481 ggtaatcttc atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc
     3541 tacccccagg cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt
     3601 cgaggatttt ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc
     3661 actgatgcat atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca
     3721 gtgcttcgcc aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg
     3781 ctcacgcgca tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac
     3841 gcgcgcctgg cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc
     3901 gtcttcctgc tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca
     3961 ggtggtgcaa gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc
     4021 tgcgtcgaga atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc
     4081 ctgatgaggc aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca
     4141 gcgcacgacg aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta
     4201 cgtttttaat cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt
     4261 gagcggcagc attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag
     4321 ccagcgcgcc gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca
     4381 tacgggcttt tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt
     4441 cgctatcggt cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt
     4501 tctccatcgc cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat
     4561 gcgcaccgcc agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc
     4621 agtcctgcac cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat
     4681 ctttaaaggt ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca
     4741 ctttctccac caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca
     4801 gcagcatttc gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa
     4861 tagcgacgac tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta
     4921 cccgttcacg caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt
     4981 cgtcagcttc tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta
     5041 ccagtggcgc gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa
     5101 cgttaacggt cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca
     5161 gcagttcacg ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt
     5221 aatccgccag cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca
     5281 gcacttcaaa cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga
     5341 ggtcataaac agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag
     5401 cagtcggttc gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg
     5461 tgccctgacg ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct
     5521 cgcctgccag ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc
     5581 gcaccgggtt cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct
     5641 ggaattgata aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca
     5701 ggcgtttaac agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat
     5761 aacccaccga atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt
     5821 catggtcggc taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg
     5881 tgcccaggtc aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt
     5941 cactaatttg taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct
     6001 tcgagttgtt cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc
     6061 gccgccgcgt cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca
     6121 aacatctttt tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg
     6181 atctcgtcca gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca
     6241 gtatgctgct cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt
     6301 aacggatgac gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg
     6361 agttgttccg ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc
     6421 tgaaaacgca ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg
     6481 gtgaagtaat ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta
     6541 aaacaaaccc cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga
     6601 agctttcgcc gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat
     6661 tcaggccttc ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat
     6721 cgaccacgac tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa
     6781 caaattccag tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc
     6841 caaacccttt gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca
     6901 gtgtaatcga cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc
     6961 gcaatggcgg ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc
     7021 agttcaagtt cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc
     7081 cctttcaccc attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt
     7141 ttaaaacgcg cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg
     7201 tcgccacagg ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag
     7261 gaacccacgt tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata
     7321 aattctcctg attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg
     7381 ccctgcttgt acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga
     7441 actaactcga tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg
     7501 atagagctat gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg
     7561 aggcttgctg acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc
     7621 agcgactcac cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc
     7681 aggtcaccgt tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc
     7741 aggccgcgca gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg
     7801 cccattccga cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg
     7861 ccgccgtgca tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg
     7921 atacctttcg ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg
     7981 ctcaggtcga ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca
     8041 cgagcacggc acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt
     8101 acgtgcatga tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg
     8161 tcgataatac cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc
     8221 tgacggcaag tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct
     8281 tttttctgat aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca
     8341 gaggtaaaga cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg
     8401 atatctaccg cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca
     8461 aaggttccgt ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc
     8521 gtggttgcgg agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc
     8581 actcaatgca aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag
     8641 cactccggcc tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct
     8701 tgtgtgcggg tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc
     8761 tggttattaa ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc
     8821 aaatcacgcc acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc
     8881 tgacaacggg tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg
     8941 ctgctggcat ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga
     9001 ccatttttac gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt
     9061 tcggaaatat cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt
     9121 gcggtcacgg catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg
     9181 cccctgcccg gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga
     9241 cattcccgag taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc
     9301 agtgccattt ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat
     9361 ccgacctacg attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca
     9421 accactgtta ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc
     9481 gcaggatatt caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat
     9541 tcatcacctg ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca
     9601 gatgaccata aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga
     9661 cctgctcgcc attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct
     9721 gaaccgccat tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat
     9781 aatggcattt ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg
     9841 ccaccggggt atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca
     9901 tcggccacgg cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta
     9961 acgcttcgtc gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag
    10021 ccagggcaat cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta
    10081 atcccattgt tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca
    10141 ccagcacaat tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat
    10201 cttatcataa aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt
    10261 tctttaacat ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag
    10321 cgcgcaaggc gggtaattta gttgccaaaa actatgaaac cccggacgct gtagaagcga
    10381 gccagaaagg cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta
    10441 tcgacacgat tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac
    10501 ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact
    10561 ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg
    10621 aagttgctgt ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag
    10681 gcgcacagct gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca
    10741 ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg
    10801 tcggcaagct gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc
    10861 tggcttacgt cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg
    10921 atttcgcggc aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg
    10981 gtggtcataa ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag
    11041 ccatactggc gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc
    11101 gggtgatatc actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg
    11161 ctttactttc tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt
    11221 cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc
    11281 tctttgttgt tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt
    11341 atcctgacga taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg
    11401 ctaaggacgg tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca
    11461 acgccatcgc aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc
    11521 cgctggtcag ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt
    11581 ttggtaaatc aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca
    11641 tcaatgtggt gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga
    11701 gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg
    11761 cggtgatcct cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg
    11821 gcagtggcta cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc
    11881 cgatcccgct tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg
    11941 aaaagttgta tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac
    12001 acattgatgc tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta
    12061 tccttagcgc gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca
    12121 aaaccacctt aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg
    12181 tgacgcttgc caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt
    12241 tagctattct tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca
    12301 ccatctgtcc tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg
    12361 gggttgaccg ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc
    12421 agtcttgata tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg
    12481 actggttatg acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta
    12541 tttcactggc tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc
    12601 cccgccctga agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc
    12661 aatctacaag cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc
    12721 gatattcatt tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt
    12781 acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta
    12841 gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag
    12901 ccactgttta tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg
    12961 catcagcggg acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag
    13021 ctgggaaatg ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc
    13081 gcccaggcgt taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag
    13141 ttcgccagac tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa
    13201 agtgaatacg gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt
    13261 gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat
    13321 gatagcgagc gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc
    13381 aacattaagc atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca
    13441 ctgattattg cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg
    13501 gacctgacgc tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga
    13561 gggaacaact ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa
    13621 cacgccgtgg cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact
    13681 gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg
    13741 agcagcaata taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat
    13801 ttgcaccact atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac
    13861 gccgcaaatc gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt
    13921 actcagcgca aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc
    13981 cgaggccgag tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg
    14041 tgccccctgc aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa
    14101 ccacgcagac caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg
    14161 acggatgaga aagccgagca gcatggatgc cacgcccaac gtcaacagca cgagaatcac
    14221 ccgataatca aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac
    14281 cgagccccac agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc
    14341 cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag
    14401 catcagccac gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag
    14461 tgccagcacg cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag
    14521 caaactcccg agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg
    14581 cgttaaacca atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa
    14641 aaagtatgtg aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc
    14701 ccgtcaggag gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat
    14761 attcgagcct gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc
    14821 cgcgacgcat gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg
    14881 cacaatggcg atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag
    14941 taaagcaatg gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa
    15001 attattcata taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt
    15061 caccagaata ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca
    15121 cgctttaacg atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac
    15181 gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa
    15241 caccaccact aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg
    15301 atggcgcatt aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt
    15361 gattaaactc accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac
    15421 ttttggcaat aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc
    15481 ctgaacaatt ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc
    15541 gtcctggaga aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag
    15601 taacggaaca ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga
    15661 ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata
    15721 gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta
    15781 gaaattaact atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag
    15841 aaggtcaata catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac
    15901 atttaccaac tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg
    15961 gacatttacc aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac
    16021 gaaagccaga tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt
    16081 gtcgtggtgc gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg
    16141 gccatgcgtg tgagttatgc agattgtggc aacactcgcg cctttacctg cccgtatcac
    16201 ggctggtctt atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac
    16261 ccacaagggt tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat
    16321 aaaggactga tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt
    16381 gacattgcct ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc
    16441 ggcggcgtac aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc
    16501 agtgaccagt atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa
    16561 gatgatggca gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc
    16621 gccaaagatg cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa
    16681 aaaccggatg ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat
    16741 gccgaagcag aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat
    16801 atattcccca cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc
    16861 ggccctgatc aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa
    16921 gttaaagccg cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag
    16981 caggatgact cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc
    17041 aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt
    17101 cctggcatta ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg
    17161 gccgatcttc tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag
    17221 caggaggtga tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt
    17281 ttctctttca cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc
    17341 tcgacgaaga gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc
    17401 gcaaaggcgt ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc
    17461 ggcgaatcgc ccgtctggaa acgggcattg cctgggcaga agagccgccg tcacgcactc
    17521 gtcacttaat cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga
    17581 gggtgaatta tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa
    17641 cgcgtttcga caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata
    17701 tcgtcctgga tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg
    17761 aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc
    17821 gcccgtcatc gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag
    17881 ccatggtaat gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc
    17941 gctacacgcc gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga
    18001 tccgctcacc acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga
    18061 ggcgcagcca tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga
    18121 ttagggctgg cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa
    18181 ctgtcggcgg caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg
    18241 gaaggtaacg tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt
    18301 tccggcaagc tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg
    18361 gttaatactc ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta
    18421 ctcggttacc tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc
    18481 atgattttca cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc
    18541 accagtaaac atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa
    18601 gtgcgggtga atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg
    18661 ctcgggcaaa gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt
    18721 ttaccgctgc aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca
    18781 tcgcggcgca ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg
    18841 attcgcggca ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa
    18901 aaaacgatca ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc
    18961 cagcaagggt tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa
    19021 cgacctccac tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta
    19081 cccgctaact ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg
    19141 ctgggtcgcg acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag
    19201 ctttttatag caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa
    19261 cgctgcttta ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc
    19321 gaacggtcag tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc
    19381 acgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat gggccgtaat
    19441 gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg tgtgcgcatt
    19501 ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact gacgctgcaa
    19561 agtggggaaa cgcttcaggc tgatgtggtg atttacggta ttggtatcag cgccaacgag
    19621 caactggctc gcgaggccaa ccttgatact gccaatggca ttgtcattga tgaggcttgc
    19681 cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg tcttgataat
    19741 ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca aattgccgct
    19801 gccgcaatgt tagggctacc gctaccgcta ctgccgccgc cgtggttctg gagcgatcag
    19861 tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct ttgtcgtggc
    19921 aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct tatcggtgcg
    19981 gtcacgctga atcagggccg tgagattcgc ccaattcgca aatggatcca gagcggcaaa
    20041 acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact gtaatcagga
    20101 taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg tattacgttt
    20161 aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt tgattttgga
    20221 gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat tttcattatt
    20281 tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc ctcgttgggc
    20341 gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc cgccgcgata
    20401 ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt ctacacgctg
    20461 ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt tgggccaaat
    20521 atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt ggcaattacc
    20581 ggaccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg gcgtgaacgc
    20641 cttatccggc attttataaa ttacagccac tctacccgca acgacatctc tgaggttgtt
    20701 gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg atcatccggc
    20761 gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc aaacgcaggg
    20821 tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat gatggcataa
    20881 ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc attgttcacc
    20941 cactgacgcg gcaacggcgc gagctggtta aaatccagtt cctgcggtag ctgctcgcaa
    21001 aactcacccg ccagccactg ctcccgttcc agccagtaac cgctcgcctg cgcctgaatc
    21061 cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg taccaaatgg cagcgtctct
    21121 gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa atgaaacgcc
    21181 tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca caaactatca
    21241 tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag atagtgcgca
    21301 tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa acggttgccg
    21361 ctcacccggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc cacaccgctt
    21421 tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat tacgccgccc
    21481 tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt catccgctaa
    21541 ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc atcagcacat
    21601 aggcatcacc tgcctgttct agcgagaagc gatgggtaat ggcgttacgc ggccacagct
    21661 tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc acccaggagc
    21721 cgataatccg ccgttgatgg tgcatcagat cagcgctgac ctcgaattcc acttttccgg
    21781 tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc agcaagcgac
    21841 ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta agttcagcaa
    21901 ttatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcccc cctaactgtt
    21961 ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt gcaccgcgac
    22021 ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc agcacgttat
    22081 cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg ccgcaactga
    22141 taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct ttttcttccg
    22201 ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct tttcctttgc
    22261 cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag ccagaaatgt
    22321 gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc atcgccacaa
    22381 tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta tcgggtgccg
    22441 ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa atcccggagg
    22501 atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact tcccgcagat
    22561 cgacggttga atttcctagt aaataagcag ccagcatcgt tttcataaat ccctcattaa
    22621 caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt
    22681 cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca cgatgatgac
    22741 gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac cgttaccgat
    22801 tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac cgccggaaag
    22861 tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc
    22921 tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg gtcaccgccg ctaataatcc
    22981 cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca actgcgccgc
    23041 cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc ggctaatgaa
    23101 cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac cgagaaattg
    23161 tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg cgttggtcat
    23221 aaacagcccc attccgcgca gagcgctcca cagccccagt gtggcaacga aacttggtac
    23281 gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcgccta acagcaacac
    23341 cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca ggcacaccga
    23401 gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa taatcagcgt
    23461 catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca tctggttatt
    23521 caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg caatcaccac
    23581 cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga cgctcttgcc
    23641 ctgcggcaat ggtaatgacg aagcatacat cttcaggttc ctcggtaaaa ttcatcagtg
    23701 tacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa actcctgcga
    23761 gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaatt cctccacttc
    23821 actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa tacggtaaat
    23881 ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca aaatctggct
    23941 ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag aaagcgtgcc
    24001 gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct cttcggtcag
    24061 gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc gattggtcag
    24121 cactgtattt tcgtcaacgc ctagccaggg aatgatcccc gcttctttgc ggttttctgg
    24181 cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga ttttctcgcc
    24241 gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct tcagcaattc
    24301 actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac gtagcgtaaa
    24361 gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta cggcctgatc
    24421 cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga tcacgcccta gcattagcga
    24481 cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct gaccatcgcg
    24541 cataacggtg gcataggagg caatgcggcg gatttcttcc atccggtggc tgacataaat
    24601 caccgccacg cccagtgccg acatcttttt caccgcactg atcaccagtt caacttctgc
    24661 actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc ccttcatcac
    24721 ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc gttgaaacaa gttgttcagg
    24781 actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca tttgcaggta
    24841 atcaatcatg ccgttgcggc gaggccattg accaaggcag aggttttctg ccaccgtcag
    24901 cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag cggcacggcg
    24961 agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat caccgctatc
    25021 cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc cgtttttacc
    25081 taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca gcgcaacgac
    25141 gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct ctgttgccgt
    25201 gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc gttgccagcc
    25261 actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc actttttcac
    25321 cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg cccattttct
    25381 taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc tgagcaattt
    25441 cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga ttacgtaccg
    25501 cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg agatccggcg
    25561 tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc ccttcctggt
    25621 tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat cctttacgtc
    25681 gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc tggtcaattt
    25741 tattggcaat aaaataatcg gcggcagcgt tacccagttt tttaccaaat tccagtggat
    25801 cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata caggtgttgt
    25861 agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta ctgccatttt
    25921 cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta tcaacaaagg
    25981 tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc tgtactgaag
    26041 aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc tgggtatcaa
    26101 ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt gcgctaccta
    26161 atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc atagtgcctc
    26221 ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc ggcagcgcgt
    26281 gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata aaaggcatca
    26341 cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat tcagcgcgag
    26401 cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc accatcaggc
    26461 atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca ggttgtgaaa
    26521 cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac tgtttcattt
    26581 cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc attccttgcc
    26641 agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg gtgcgatcgc
    26701 cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc gcgttggcgc
    26761 atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg ccttcgctta
    26821 aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg
    26881 catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg gtgaccttcc
    26941 cttccccgcc ttcccacggg tagaatttac gcgtggcgag aatgtccgcc gctttgtctg
    27001 cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg cgtttcagcg
    27061 caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct ccacttaact
    27121 tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc tgataagcat
    27181 tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca tggatcgcta
    27241 acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc cataaggcaa
    27301 tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg cgagcaaacc
    27361 agcactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg aaacggacaa
    27421 actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct ttcggcagca
    27481 agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc agcatctctg
    27541 ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga gcgttaacgt
    27601 cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga ccatcgaacc
    27661 agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc tcgcgctgca
    27721 aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag caaagcactt
    27781 cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc gcgtcgaagt
    27841 tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta ctattgccgc
    27901 tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga cgttcgtaag
    27961 cactggcgcg aatcaaactt gcttgtccac actgcgggtt tttgttcagc gcatgtgcgc
    28021 gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg ttatattcca
    28081 gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg ccgcgcaggt
    28141 agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga ccgataaacc
    28201 aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg acgtccggca
    28261 acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg tcggcatcgg
    28321 agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg atggcggtcg
    28381 ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt tcgcggatcg
    28441 ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc ccccgctcac
    28501 tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc agagaatgat
    28561 aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag gtaaaatcag
    28621 gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta ttgtcggtca
    28681 gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt tttttacctg
    28741 gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga caccacgcgc
    28801 cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc acatttttat
    28861 agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg gtggcgatgg
    28921 ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc ggtgggaaaa
    28981 cgctctgatg cccttcaccc cctttcactg ccgggttggc ccaccacaag aaatgacgcg
    29041 gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg tcagggcgca
    29101 gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg acccacaccg
    29161 tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc
    29221 gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc agcatcccca
    29281 ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc actttatccc
    29341 atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg tagtcgtttt
    29401 ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta tcggtcacgc
    29461 cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg aacatgggat
    29521 gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc cacactttta
    29581 ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat ttgtggcagt
    29641 gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta aacaaggttt
    29701 agtgaagatg agagcctgca ttaacaatca acagattctc caccataaca aatgcgtgat
    29761 tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc ggctggcgca
    29821 aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt
    29881 gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc
    29941 ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt gtcaggttgc
    30001 taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg taccgacgcc
    30061 gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga
    30121 ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga ccggcgtatc
    30181 gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc tgctggaaga
    30241 gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc tggcggagaa
    30301 atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aaagttgatt acggcattgg
    30361 ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta gcggacagat
    30421 tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt acggcagcct
    30481 ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc taaaatcaca
    30541 accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa tcgctgcctg
    30601 gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg ccattggttt
    30661 gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt acggtcgcag
    30721 ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat ttaacccgtt
    30781 cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc aattaagccg
    30841 cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac gacagatttg
    30901 atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata aacgaaaaat
    30961 tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg aatttgcaag
    31021 attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga gcacattgtc
    31081 agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt
    31141 acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt cacacatcca
    31201 gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg ccggagtacc
    31261 tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc tgtttttgtt
    31321 gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca ggtttttatc
    31381 aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt tgtagccgcg
    31441 ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct ggtaagtttt
    31501 gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa
    31561 cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca gctcttcgct
    31621 accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt gagtggtggt
    31681 agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc
    31741 aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt cacgcatttt
    31801 cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt tcggcttgtg
    31861 ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat cgataccgta
    31921 aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag tcaggtgacc
    31981 gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg cggtgtagac
    32041 cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag
    32101 ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc aaccgccgta
    32161 gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac cctgcgcctg
    32221 cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct cttcctgacg
    32281 tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa tgttcatttc
    32341 acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc ctttataggc
    32401 ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac aggtcttgac
    32461 aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga ataaagagct
    32521 tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact tcatcacatt
    32581 ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat aagatgcatt
    32641 tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct caaaccatcg
    32701 ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta accgcccatt
    32761 tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac atgagtaacc
    32821 agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac gccagtaata
    32881 ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag cacaccagct
    32941 tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca acgctggacg
    33001 aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat ggtgtactgg
    33061 ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc aaagccggtg
    33121 gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc gcgcttatca
    33181 ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt ccggggcaat
    33241 atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt cagtactctt
    33301 tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa gagggtgggc
    33361 aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc
    33421 cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta atctctaccg
    33481 gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca ggccacacag
    33541 cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt gctgatgaag
    33601 ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat cgtcagccga
    33661 gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat ttgagcaaac
    33721 tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg gttggcttca
    33781 tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac attcattacg
    33841 aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa catccggcaa
    33901 cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg cgaatgaccc
    33961 gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc gccgtgcgaa
    34021 taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca attttcactt
    34081 tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg cctttctggc
    34141 gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg gccagtgctt
    34201 cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc atgctattcc
    34261 ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca attcattcct
    34321 tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct gtccggttgc
    34381 gccccgccat tctagccgcg tgggtgacgt tgcctttggt gatttgcagc agcttacgca
    34441 aatagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc gtattttcac
    34501 cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc agcgccacgc
    34561 actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg gtcatcaggc
    34621 gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct gccgcctggc
    34681 gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt gccggaattt
    34741 tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg cgcgccatcg
    34801 cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca atatcgcggt
    34861 tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg acctgtaacg
    34921 gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc gcctggaata
    34981 aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca aacagctccg
    35041 actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt ttgctgttgc
    35101 gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg ctctgaccgt
    35161 taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc aaacgcagca
    35221 tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct ggcgcggatt
    35281 gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc ttggtgagga
    35341 aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca tgcgcagtaa
    35401 gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc tgcataccgt
    35461 ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga ttcagtaccc
    35521 gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg gtcaggcgca
    35581 ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc gcaggtttat
    35641 ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc gtgcggcgta
    35701 tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat atcggtcagg
    35761 ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg ttgctggcgc
    35821 aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg gcgctcttcc
    35881 gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg gcgaacctgt
    35941 gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg ctcatacggc
    36001 gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca gctgccgtcg
    36061 tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc acaatccatc
    36121 gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc ccagatgtcg
    36181 ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc ttctgctggc
    36241 gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag acatggcagc
    36301 cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga
    36361 cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg acagcgcgga
    36421 gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg ccgaactgac
    36481 aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc gacgacggta
    36541 attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt tccccttgca
    36601 tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc accgcccctt
    36661 ttcgctggtg gcttccctga aaaaaggggt cgaagatcat ggcgcgttcc tcttgcggaa
    36721 tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa ctgctgcgaa
    36781 ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga ttatccagta
    36841 cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg acgtcggtat
    36901 gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc accagcggtg
    36961 ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg taatcaagca
    37021 gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc accacctctt
    37081 tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg ccctcgcgca
    37141 tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga tggcgttggg
    37201 attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc gagcgtaact
    37261 cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc agacggttga
    37321 tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt agcaccatca
    37381 ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca cgttcggcga
    37441 tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc tgcaccattt
    37501 cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg ggaccactgt
    37561 tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg taaagtttat
    37621 cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg cgctggcttt
    37681 gataaacctt cacaagcgta gggtcgtcca gcacgcaata ctgacggtag ctacgctcca
    37741 tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc gtagtgcggt
    37801 taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc catgccagca
    37861 ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt aatgagcggg
    37921 gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa
    37981 ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta tggaataagg
    38041 cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata tcagaagttg
    38101 gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc gaaggggctg
    38161 acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc aataaagaaa
    38221 atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt cttcgctatt
    38281 tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa cttattagat
    38341 tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc acccattgtc
    38401 gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg gttggtgctt
    38461 aatgccggat gcggcgtaaa tgccgcatcc gggctgcaaa accaatgaac tgatgactta
    38521 ccccaactgc ttacgcgcat tgcggaaaat gcgcatccat gggccatcct cgccccagtt
    38581 ttccggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg gcatcataat
    38641 ggtgactcga ccactttcag tcgtaaccgc cgtaataccg ttcggtgaac cgttcgggtt
    38701 agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc
    38761 tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc cttcaccatg
    38821 agagacagca atcggcattt gcgagcccac catcccctgc aacagcagag acgggctttg
    38881 ggttacttca accaggctga aacgcgcttc aaagcgatcg gaggtgttac gcacaaaacg
    38941 tggccacaac tcactccccg ggatcagttc acgcaggtta gacatcatct ggcaaccgtt
    39001 acataccccc agggacagcg tttgcggacg atggaagaag gttgcaaact catcgcgtac
    39061 acggtcattg aacaggatag acttcgccca accttcaccg gcacccagca catcaccgta
    39121 ggagaaacct ccgcacgcga ccagggcgtg aaaatcctcc agtcccgtac gtccagtcag
    39181 caggtcgctc atatgcacgt cgatagcatc aaagccccca cgttggaaag ctgccgccat
    39241 ttcaacatgc gagttcacgc cctgctcacg cagtacggca actttcggac gtgcgccagt
    39301 ggcaatatac ggtgctgcca catcttcgtt gatatcgaac gacagtttta cattcaggcc
    39361 cggatcggcg tcgttagatt tcgcctgatg ctcctgatcg gcacactccg ggttgtcacg
    39421 caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtag tgcggctttc
    39481 gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa ccgcctgccc
    39541 aacataatgg acgcaatccg caagcccatg ctgtgccagt acggactcga ccgcttcacg
    39601 gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg ttaaacaacg ccgccaggcg
    39661 atcgtcaccc agagaggcga tatccgcgtc aatgccacaa tgacctgcaa aggccatttc
    39721 cgccagcgtt accagcaggc ctccatcaga gcggtcgtga tacgccagca gcttacgctg
    39781 tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tggacaacat cgcgtacatc
    39841 tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt
    39901 attgcctttg cccaaatcaa tcagcagcag tgcgttatct tcggtacaaa gctgcggcgt
    39961 gatggtgtga cgtacatctt ccacgcgggc aaatgcagaa atcaccagcg acagcggcga
    40021 cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca tagagtcttt
    40081 acccaccggg atcgtcaggc ccagcgccgg acaaagttct tcacccacgg ctttaacggc
    40141 ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt tggcggaaag
    40201 tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata ttggttaacg cttcaccgac
    40261 cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc
    40321 catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt tagcgaccgg
    40381 cacctgccac ggccccacca tctgatcgcg cgccaccatg ccggttacgc tgcggtcacc
    40441 gatggtcacc aggaaagttt tctccgccac ggtcggcaga tgcagtacgc gtttcaccgc
    40501 gtctgcaatg gtgatccctt cacggaccag cgcatcgccc ttcgctttca gcgtttgtac
    40561 atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat cgatcggctg
    40621 attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt caccaatcac
    40681 cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt gatcggcagc
    40741 aaccgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg ggctcatgcc
    40801 cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgtccgc cgtcgctcac
    40861 cagctccggc atcgcgttag aaagaccgcc cgcaccgacg tcgtggataa acagaattgg
    40921 gttagcatca ccaagctgcc agcaacggtc gatcacttcc tggcaacgac gctccatctc
    40981 cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac cagacgccat
    41041 ggaagacgcc gcgccgccgc caagaccgat gttcattgcc gggccgccga gaacgaccag
    41101 cttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt tgccgatccc
    41161 gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccattgt ggctgttcac
    41221 tttttcttca taagtacgga agtagccgtt cagtgccgga cgaccaaatt cgttgttaaa
    41281 cgccgcaccg cccagcgggc cttcggtcat gatgtccagc gcagtaacaa tgcgctcagg
    41341 cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt tggatacgga
    41401 gaaaccaacc aaaccagctt tcggcttcgc gccgcgcccg gtggcacctt catcgcggat
    41461 ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg
    41521 agtttcgact ttcatcagga tgtgcgccgg ttcctgatgg aaattgtagc ggcccgtttc
    41581 gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt tatctttata
    41641 agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatcc tgaacagcga
    41701 tttcggctgt tgttcaccgt cgataatcca gtcggcgtta aaaattttgt ggcggcagtg
    41761 ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac gaccaagctt
    41821 tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaagc caagacgcag
    41881 gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgcttg tgaccggagt
    41941 cggttgatgg tgggcaaaca actgctctgc atcatctaaa tcaaaaaaga ccgtttccat
    42001 catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca gcgtaccggc
    42061 ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc cgcagttatg
    42121 ggcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg gggtcaccag
    42181 caggagtttg ccttgcgggg cgtggctggc gagtgccgga ccatatttca gcaggcgttc
    42241 aagttgtgcg tgctcatcat cgtttaacga cgcattgagg tcagcaaaat ggacatactc
    42301 ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca gtttgttgat
    42361 tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc tctcgtcttc
    42421 taagctttcg gtgtacccaa ggggggggaa acgggcgtca ttataaagaa tctgatgcgc
    42481 tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg cgtgccgcca
    42541 atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc atttatgagt
    42601 aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca gagaattaac
    42661 taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact gctgctcgcg
    42721 gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat cgccgccatt
    42781 caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta taacgaaatc
    42841 aacgggaaac cttttggcct agattacgaa ctggcgaaac agtttgccga ttacctcggc
    42901 gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga ccttgataat
    42961 ggtaacgccg acctgctggc ggcaggactg gtctataaca gtgagcgggt aaaaaattat
    43021 cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt gggtcagtat
    43081 cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc gggtcatgtg
    43141 gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag ctggaaggta
    43201 gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa actcgattac
    43261 accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga gctcgccgta
    43321 gcgctcgata tcaccgatga acaaccggtg acttggttta gcccgttaga tggcgataat
    43381 accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg tacgctggca
    43441 cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga tacgcgcaca
    43501 tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga gaaatacgcc
    43561 gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca ctgggatgca
    43621 caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa
    43681 agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg cgtgcgttat
    43741 ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgaacg gatctggttt
    43801 gccctcgctg cgtacaatat gggctatgcg catatgctgg atgcccgcgc cctgacggca
    43861 aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc tttacttagc
    43921 cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga agcctacgct
    43981 tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca agagaaagag
    44041 aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt atcgcctacg
    44101 gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc atcaaactat
    44161 ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa acaaaattaa
    44221 tccgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat gcgaaagaag
    44281 tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc cactcggtga
    44341 ttcatacccg gatgatgcag cacatccatt aaagatccca cagcgccagt tttcgcgtca
    44401 cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc acacattaca
    44461 catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg catcaccaga
    44521 ccaccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatggcg accaatcggg
    44581 cggttccagc cttcgccgat tacccgattg ttatgcacta ataccgcgcc gaccggcact
    44641 tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca atattcgtgg
    44701 ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg cattatacac
    44761 ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt tactcgccaa
    44821 cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata gccactgtac
    44881 agccgcagcg gcgtgccgat tttgcgctcc agttgtgcga ccttttcatg tcccagacaa
    44941 cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat aagattaacc
    45001 cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg agagccggta
    45061 atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc
    45121 ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa cgtctgtaaa
    45181 cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca gactcatcgg ccagcgtgcc
    45241 gcacgacctt ttatcaataa cgcaatggct ataatcggta acaacggcag gacaagtaac
    45301 gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat atcctgctga
    45361 tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc caaaccatac
    45421 tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac agatagatca
    45481 tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa ttcatggcaa
    45541 ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg ttgtcgccac
    45601 tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg ttaccaaggg
    45661 cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga tacggcgcgc
    45721 catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag
    45781 ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt gagtgaagcg
    45841 ctggcaagcc agccggaatc gccctccgtg cccattcata accaaatccg cggtgatgat
    45901 ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat gtacgcaacg
    45961 ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg
    46021 cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag
    46081 ctgatgaaga ttggcttcaa tgctgccgca ctgcgcgata tgcatgcgct actcgcaaca
    46141 gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg tgtacgacgc
    46201 gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc
    46261 ggctttactc cgaatgccct gcaacagcgt gcttctcatt gcctgtatac cattgccgaa
    46321 gaacaagcga caaacagtgc ttcaatctct gcttgtcacg ctcagggaat gttaacggat
    46381 ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg tattcgtcat
    46441 agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat gcccgcccgg
    46501 tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat gcatcaattg
    46561 tgatatgtgt gaaatcaaat aaaaacacat ttattttcat agtgatacag caatgtgtta
    46621 ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc ataagttcag caacacaaag
    46681 aacaccactt tctttaacgt cctccgcgcc ctaaaggacg gggctttacg gcgcaaaagg
    46741 cctgtgacat acaacattaa cttttcagtc aaccgtagca cgttcttgta tacgccgtgt
    46801 ctgcggtttt atataaccct ggctggaaac ctcttataca aagtcgattc gccaacatca
    46861 tagatgatcg ccaccttctg gcgagaaact cctgatgcaa ttagtcgccc ggcctgcgcc
    46921 cattattctg gtgaaagttt gggacggcga ccgccaattc gtccctgtgc gcgagcagct
    46981 tccagtcctg cttttgttcg ttcaacaatc agttcacgtt ccatttcagc cagggcaccc
    47041 atcacatgga agaaaaagcg tcccattggt gtgctggtat caattgaatc agtcagacta
    47101 cgaaagttga tgcctcgttc gcgcaactcc tccaccagca cgacaagatg ccgcatactg
    47161 cgccccagcc gatccagctt ccagacaacc agtgtgtcgc ctgccgataa tgtcttgagc
    47221 agttttttca gccccggtct ttcggacttt gtaccactta tcttgtcttc aaaaatcagc
    47281 tcgcatcctg cacagttcaa cgcattacgt tgtagatcgg tattctggt
//
LOCUS       DAJTNV010000024        46596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNV010000024 DAJTNV010000000
VERSION     DAJTNV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(11..598)
                     /gene="mog"
                     /locus_tag="OO199_001331"
     CDS             complement(11..598)
                     /gene="mog"
                     /locus_tag="OO199_001331"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5535436.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(713..1666)
                     /gene="tal"
                     /locus_tag="OO199_001332"
     CDS             complement(713..1666)
                     /gene="tal"
                     /locus_tag="OO199_001332"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5535437.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1945..3374
                     /locus_tag="OO199_001333"
                     /pseudo
     CDS             1945..3374
                     /locus_tag="OO199_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3444..4220
                     /gene="yaaA"
                     /locus_tag="OO199_001334"
     CDS             3444..4220
                     /gene="yaaA"
                     /locus_tag="OO199_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5535438.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4373..4669)
                     /locus_tag="OO199_001335"
     CDS             complement(4373..4669)
                     /locus_tag="OO199_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5535439.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4883..6169)
                     /gene="thrC"
                     /locus_tag="OO199_001336"
     CDS             complement(4883..6169)
                     /gene="thrC"
                     /locus_tag="OO199_001336"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5535440.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6170..7102)
                     /gene="thrB"
                     /locus_tag="OO199_001337"
     CDS             complement(6170..7102)
                     /gene="thrB"
                     /locus_tag="OO199_001337"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5535441.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7104..9566)
                     /gene="thrA"
                     /locus_tag="OO199_001338"
     CDS             complement(7104..9566)
                     /gene="thrA"
                     /locus_tag="OO199_001338"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5535442.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9592..9711)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9647..9712)
                     /gene="thrL"
                     /locus_tag="OO199_001339"
     CDS             complement(9647..9712)
                     /gene="thrL"
                     /locus_tag="OO199_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5535443.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9926..10612)
                     /locus_tag="OO199_001340"
     CDS             complement(9926..10612)
                     /locus_tag="OO199_001340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5535444.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11012..11152)
                     /locus_tag="OO199_001341"
     CDS             complement(11012..11152)
                     /locus_tag="OO199_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535445.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11248..11964
                     /gene="arcA"
                     /locus_tag="OO199_001342"
     CDS             11248..11964
                     /gene="arcA"
                     /locus_tag="OO199_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5535446.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12023..13375)
                     /gene="creD"
                     /locus_tag="OO199_001343"
     CDS             complement(12023..13375)
                     /gene="creD"
                     /locus_tag="OO199_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5535447.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13433..14857)
                     /gene="creC"
                     /locus_tag="OO199_001344"
     CDS             complement(13433..14857)
                     /gene="creC"
                     /locus_tag="OO199_001344"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5535448.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14857..15546)
                     /gene="creB"
                     /locus_tag="OO199_001345"
     CDS             complement(14857..15546)
                     /gene="creB"
                     /locus_tag="OO199_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5535449.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15559..16032)
                     /gene="creA"
                     /locus_tag="OO199_001346"
     CDS             complement(15559..16032)
                     /gene="creA"
                     /locus_tag="OO199_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5535450.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16243..17112
                     /gene="robA"
                     /locus_tag="OO199_001347"
     CDS             16243..17112
                     /gene="robA"
                     /locus_tag="OO199_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5535451.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17109..17756)
                     /gene="gpmB"
                     /locus_tag="OO199_001348"
     CDS             complement(17109..17756)
                     /gene="gpmB"
                     /locus_tag="OO199_001348"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5535452.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17808..18328
                     /gene="yjjX"
                     /locus_tag="OO199_001349"
                     /pseudo
     CDS             17808..18328
                     /gene="yjjX"
                     /locus_tag="OO199_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18413..18739)
                     /gene="trpR"
                     /locus_tag="OO199_001350"
     CDS             complement(18413..18739)
                     /gene="trpR"
                     /locus_tag="OO199_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5535453.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18829..20766)
                     /gene="sltY"
                     /locus_tag="OO199_001351"
     CDS             complement(18829..20766)
                     /gene="sltY"
                     /locus_tag="OO199_001351"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5535454.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20977..22644
                     /gene="ettA"
                     /locus_tag="OO199_001352"
     CDS             20977..22644
                     /gene="ettA"
                     /locus_tag="OO199_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5535455.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22751..22918)
                     /locus_tag="OO199_001353"
     CDS             complement(22751..22918)
                     /locus_tag="OO199_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535456.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22952..24184)
                     /gene="nadR"
                     /locus_tag="OO199_001354"
     CDS             complement(22952..24184)
                     /gene="nadR"
                     /locus_tag="OO199_001354"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5535457.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24205..25587)
                     /gene="radA"
                     /locus_tag="OO199_001355"
     CDS             complement(24205..25587)
                     /gene="radA"
                     /locus_tag="OO199_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5535458.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25636..26604)
                     /gene="serB"
                     /locus_tag="OO199_001356"
     CDS             complement(25636..26604)
                     /gene="serB"
                     /locus_tag="OO199_001356"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5535459.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26710..27354
                     /locus_tag="OO199_001357"
     CDS             26710..27354
                     /locus_tag="OO199_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5535460.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27382..28397
                     /gene="lplA"
                     /locus_tag="OO199_001358"
                     /pseudo
     CDS             27382..28397
                     /gene="lplA"
                     /locus_tag="OO199_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28429..28692
                     /locus_tag="OO199_001359"
     CDS             28429..28692
                     /locus_tag="OO199_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5535461.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28853..29572)
                     /gene="deoD"
                     /locus_tag="OO199_001360"
     CDS             complement(28853..29572)
                     /gene="deoD"
                     /locus_tag="OO199_001360"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5535462.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29629..30852)
                     /gene="deoB"
                     /locus_tag="OO199_001361"
     CDS             complement(29629..30852)
                     /gene="deoB"
                     /locus_tag="OO199_001361"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5535463.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30904..32226)
                     /gene="deoA"
                     /locus_tag="OO199_001362"
     CDS             complement(30904..32226)
                     /gene="deoA"
                     /locus_tag="OO199_001362"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5535464.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32353..33132)
                     /gene="deoC"
                     /locus_tag="OO199_001363"
     CDS             complement(32353..33132)
                     /gene="deoC"
                     /locus_tag="OO199_001363"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5535465.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33390..34931
                     /locus_tag="OO199_001364"
     CDS             33390..34931
                     /locus_tag="OO199_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5535466.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34903..35766
                     /locus_tag="OO199_001365"
     CDS             34903..35766
                     /locus_tag="OO199_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5535467.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35980..36762)
                     /locus_tag="OO199_001366"
     CDS             complement(35980..36762)
                     /locus_tag="OO199_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5535468.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36759..37832)
                     /locus_tag="OO199_001367"
     CDS             complement(36759..37832)
                     /locus_tag="OO199_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5535469.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37954..38115)
                     /locus_tag="OO199_001368"
     CDS             complement(37954..38115)
                     /locus_tag="OO199_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5535470.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38232..38837)
                     /gene="osmY"
                     /locus_tag="OO199_001369"
     CDS             complement(38232..38837)
                     /gene="osmY"
                     /locus_tag="OO199_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5535471.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39230..40819)
                     /gene="prfC"
                     /locus_tag="OO199_001370"
     CDS             complement(39230..40819)
                     /gene="prfC"
                     /locus_tag="OO199_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5535472.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40910..41587)
                     /gene="yjjG"
                     /locus_tag="OO199_001371"
     CDS             complement(40910..41587)
                     /gene="yjjG"
                     /locus_tag="OO199_001371"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5535473.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41602..42048)
                     /gene="rimI"
                     /locus_tag="OO199_001372"
     CDS             complement(41602..42048)
                     /gene="rimI"
                     /locus_tag="OO199_001372"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5535474.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42017..42430)
                     /gene="holD"
                     /locus_tag="OO199_001373"
     CDS             complement(42017..42430)
                     /gene="holD"
                     /locus_tag="OO199_001373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5535475.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42533..43564
                     /gene="rsmC"
                     /locus_tag="OO199_001374"
     CDS             42533..43564
                     /gene="rsmC"
                     /locus_tag="OO199_001374"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5535476.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43841..43927
                     /locus_tag="OO199_001375"
     tRNA            43841..43927
                     /locus_tag="OO199_001375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43875..43877,aa:Leu,seq:cag)
     gene            43956..44042
                     /locus_tag="OO199_001376"
     tRNA            43956..44042
                     /locus_tag="OO199_001376"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43990..43992,aa:Leu,seq:cag)
     gene            44077..44163
                     /locus_tag="OO199_001377"
     tRNA            44077..44163
                     /locus_tag="OO199_001377"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44111..44113,aa:Leu,seq:cag)
     gene            complement(44202..44438)
                     /locus_tag="OO199_001378"
     CDS             complement(44202..44438)
                     /locus_tag="OO199_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5535477.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44579..45367
                     /gene="fhuF"
                     /locus_tag="OO199_001379"
     CDS             44579..45367
                     /gene="fhuF"
                     /locus_tag="OO199_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5535478.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(45405..46082)
                     /gene="bglJ"
                     /locus_tag="OO199_001380"
     CDS             complement(45405..46082)
                     /gene="bglJ"
                     /locus_tag="OO199_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5535479.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46040..>46596)
                     /locus_tag="OO199_001381"
     CDS             complement(46040..>46596)
                     /locus_tag="OO199_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5535480.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11040 a        12694 c        11726 g        11136 t
ORIGIN      
        1 ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca
       61 cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
      121 caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
      181 taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcacgccca
      241 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
      301 caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
      361 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
      421 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
      481 acggcgtggt tagcgccgat gtcagccatt cttccagcgc tggaatgcct ttatcctgat
      541 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
      601 agatttttcc gtttaaacaa tctcgttaaa cagaatgata cactgcgaag ggagtgacag
      661 acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca
      721 gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg
      781 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
      841 tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt
      901 cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca cagcctgcca
      961 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt
     1021 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     1081 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     1141 agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     1201 tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata ccctgccagg
     1261 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
     1321 tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt
     1381 cagttgagat acggcccgga accagtttca ggatttccag accaatattt actgccagtt
     1441 tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
     1501 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
     1561 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
     1621 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
     1681 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
     1741 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
     1801 cgttgatatc gcccaccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa
     1861 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
     1921 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
     1981 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
     2041 ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat
     2101 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg
     2161 ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc
     2221 ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga atgttccctt
     2281 gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat
     2341 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
     2401 gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt
     2461 tcttttgatt ttcctccgct ggtgacgggc attattctcg ctgtctttgc tctgctggca
     2521 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg
     2581 ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac
     2641 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg
     2701 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
     2761 gtgggaatgg gatcgacgcc aaacgcggca gcggcagctg catcctggcc tccgcatccg
     2821 gcagcacaag ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg
     2881 gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt
     2941 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc tgaatttgtt
     3001 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     3061 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc
     3121 accttcgcaa cgatcatcgg cggcaccttg ctaagccttc cgctgatgtg gcaactggca
     3181 gatatataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
     3241 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
     3301 ttgatccgtt gcgttatccg gatatcggtc gccaactttc tccggacgcg tgggatgatg
     3361 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
     3421 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
     3481 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
     3541 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
     3601 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
     3661 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
     3721 ccgagacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
     3781 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
     3841 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
     3901 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
     3961 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
     4021 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
     4081 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
     4141 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
     4201 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
     4261 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
     4321 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
     4381 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
     4441 gcggtgacgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
     4501 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
     4561 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
     4621 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
     4681 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
     4741 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
     4801 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
     4861 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
     4921 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
     4981 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
     5041 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
     5101 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
     5161 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
     5221 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
     5281 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
     5341 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
     5401 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
     5461 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
     5521 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
     5581 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
     5641 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
     5701 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
     5761 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
     5821 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
     5881 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
     5941 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
     6001 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
     6061 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
     6121 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
     6181 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
     6241 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
     6301 cgaacaaggt cgggccggag ccggagatac cgctcactac cgcgccgatt tccgcgaccg
     6361 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
     6421 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
     6481 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
     6541 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
     6601 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
     6661 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
     6721 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
     6781 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
     6841 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
     6901 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt
     6961 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
     7021 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
     7081 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
     7141 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
     7201 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
     7261 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
     7321 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
     7381 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
     7441 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
     7501 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
     7561 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
     7621 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
     7681 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
     7741 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
     7801 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg taacaacgtg gaaaccttcg
     7861 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
     7921 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
     7981 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
     8041 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
     8101 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
     8161 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
     8221 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
     8281 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
     8341 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
     8401 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
     8461 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
     8521 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
     8581 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
     8641 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
     8701 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
     8761 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
     8821 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
     8881 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
     8941 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
     9001 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
     9061 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
     9121 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
     9181 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
     9241 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
     9301 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
     9361 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
     9421 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
     9481 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
     9541 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
     9601 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
     9661 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
     9721 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
     9781 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
     9841 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
     9901 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
     9961 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    10021 cgaccagttt tatgtcatct gccactgcca gagccgtcag caatgccatg actcgttcgc
    10081 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    10141 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    10201 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    10261 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    10321 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    10381 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    10441 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    10501 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    10561 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    10621 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    10681 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    10741 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    10801 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    10861 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    10921 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    10981 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    11041 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    11101 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    11161 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    11221 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    11281 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    11341 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    11401 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    11461 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    11521 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    11581 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    11641 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    11701 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    11761 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    11821 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    11881 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    11941 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    12001 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    12061 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    12121 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    12181 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg ataaacaaca
    12241 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    12301 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    12361 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    12421 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    12481 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    12541 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    12601 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    12661 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    12721 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    12781 tattcagctg caggttctgc ttacgccagt ccccttcggg taaagggata tgcacgccct
    12841 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    12901 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    12961 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    13021 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    13081 tctgattgcc atcaaccatc aatgactcag gtaacaaaaa atggataaaa cttcgtttcc
    13141 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    13201 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    13261 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    13321 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    13381 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    13441 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    13501 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    13561 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    13621 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    13681 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    13741 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    13801 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    13861 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    13921 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    13981 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    14041 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    14101 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    14161 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    14221 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    14281 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    14341 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    14401 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    14461 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    14521 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    14581 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    14641 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    14701 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    14761 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    14821 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    14881 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    14941 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    15001 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    15061 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    15121 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    15181 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    15241 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    15301 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    15361 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    15421 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    15481 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    15541 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    15601 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    15661 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    15721 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    15781 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    15841 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    15901 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    15961 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    16021 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    16081 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    16141 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    16201 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    16261 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    16321 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    16381 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    16441 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    16501 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    16561 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    16621 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    16681 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    16741 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    16801 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    16861 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    16921 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    16981 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    17041 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    17101 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    17161 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    17221 atagaacagt tacgcagacg taagcggcgc tctacccatg ctggtaatcc gagaatcgta
    17281 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    17341 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    17401 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    17461 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    17521 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    17581 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    17641 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    17701 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    17761 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    17821 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    17881 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    17941 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    18001 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    18061 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    18121 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    18181 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    18241 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    18301 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    18361 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    18421 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    18481 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    18541 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    18601 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    18661 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    18721 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    18781 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    18841 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    18901 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    18961 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    19021 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    19081 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    19141 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    19201 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    19261 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    19321 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    19381 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    19441 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    19501 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    19561 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    19621 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    19681 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    19741 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    19801 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    19861 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    19921 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    19981 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    20041 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    20101 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    20161 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    20221 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    20281 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    20341 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    20401 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    20461 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    20521 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    20581 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    20641 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    20701 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    20761 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    20821 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    20881 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    20941 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    21001 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    21061 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    21121 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    21181 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    21241 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    21301 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    21361 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    21421 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    21481 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    21541 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    21601 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    21661 atcctcgaac tggaccgcgg tgaaggtatt ccgtgggaag gtaactactc ctcctggctg
    21721 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    21781 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    21841 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    21901 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    21961 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    22021 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    22081 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    22141 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    22201 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    22261 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    22321 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    22381 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    22441 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    22501 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    22561 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    22621 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    22681 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    22741 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    22801 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    22861 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    22921 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    22981 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    23041 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    23101 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    23161 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    23221 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    23281 cgtatttcac tgcaaaatca atgtattgtg cgtggcccaa cgcgatttta tcgtagtcgg
    23341 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    23401 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    23461 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    23521 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    23581 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    23641 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    23701 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    23761 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    23821 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    23881 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    23941 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    24001 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    24061 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    24121 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    24181 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    24241 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    24301 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    24361 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    24421 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgc cgctggtttc
    24481 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    24541 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    24601 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    24661 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    24721 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    24781 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    24841 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    24901 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    24961 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    25021 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    25081 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    25141 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    25201 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    25261 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    25321 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    25381 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    25441 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    25501 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    25561 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    25621 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    25681 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    25741 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    25801 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    25861 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    25921 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    25981 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    26041 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    26101 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    26161 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    26221 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    26281 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    26341 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    26401 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    26461 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    26521 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    26581 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    26641 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    26701 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    26761 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    26821 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    26881 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    26941 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    27001 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    27061 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    27121 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    27181 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    27241 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    27301 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    27361 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    27421 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    27481 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    27541 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    27601 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    27661 ccggagtacg ataaaactct ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    27721 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    27781 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    27841 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    27901 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    27961 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    28021 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    28081 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    28141 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    28201 gccatatcac ccgcgcccag gtatttaccg acagcctcaa cctcgcgccg ctggaagccc
    28261 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    28321 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    28381 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    28441 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    28501 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    28561 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    28621 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    28681 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    28741 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    28801 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    28861 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    28921 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    28981 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    29041 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    29101 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    29161 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    29221 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    29281 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    29341 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    29401 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    29461 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    29521 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    29581 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    29641 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    29701 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    29761 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    29821 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    29881 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    29941 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    30001 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    30061 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    30121 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    30181 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    30241 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    30301 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    30361 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    30421 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    30481 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    30541 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    30601 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    30661 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    30721 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    30781 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    30841 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    30901 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    30961 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    31021 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    31081 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaaccac
    31141 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    31201 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    31261 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    31321 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcagcat
    31381 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    31441 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    31501 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    31561 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    31621 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    31681 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    31741 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    31801 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    31861 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    31921 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    31981 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    32041 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    32101 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    32161 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    32221 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    32281 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    32341 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    32401 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    32461 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    32521 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    32581 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    32641 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    32701 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    32761 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    32821 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    32881 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    32941 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    33001 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    33061 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    33121 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    33181 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    33241 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    33301 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    33361 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    33421 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    33481 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    33541 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    33601 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    33661 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    33721 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    33781 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    33841 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    33901 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    33961 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    34021 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    34081 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    34141 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    34201 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    34261 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    34321 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    34381 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    34441 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    34501 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    34561 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    34621 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    34681 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    34741 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    34801 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    34861 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    34921 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    34981 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    35041 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    35101 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    35161 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    35221 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    35281 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    35341 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    35401 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    35461 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    35521 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    35581 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    35641 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    35701 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    35761 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    35821 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    35881 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    35941 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    36001 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    36061 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    36121 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    36181 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    36241 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    36301 accagcacgc ggcaaatcgt ggtgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    36361 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    36421 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    36481 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    36541 gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg gttgataatt
    36601 ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    36661 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    36721 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    36781 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    36841 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    36901 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    36961 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    37021 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    37081 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    37141 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    37201 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    37261 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    37321 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    37381 ggtaaaaagt aattcggctc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    37441 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    37501 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    37561 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    37621 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    37681 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    37741 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    37801 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    37861 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    37921 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    37981 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    38041 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    38101 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    38161 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    38221 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    38281 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    38341 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    38401 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    38461 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    38521 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    38581 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    38641 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    38701 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    38761 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    38821 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    38881 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    38941 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    39001 cggtagaatg acaatcgacg gctttagata ctgatatcta cgcattgaac ggtatttaac
    39061 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    39121 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    39181 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    39241 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    39301 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    39361 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    39421 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    39481 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    39541 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    39601 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    39661 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    39721 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    39781 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    39841 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    39901 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    39961 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    40021 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    40081 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    40141 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    40201 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    40261 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    40321 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    40381 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    40441 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    40501 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    40561 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    40621 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    40681 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    40741 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    40801 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    40861 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    40921 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    40981 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    41041 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    41101 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    41161 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    41221 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    41281 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    41341 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    41401 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    41461 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    41521 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    41581 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    41641 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    41701 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    41761 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    41821 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    41881 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    41941 ctggttgctg gcaaaggttt tttcactcca cggaaaggag tgggcgcgtt gttcaatgtg
    42001 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    42061 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    42121 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    42181 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    42241 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    42301 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    42361 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    42421 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    42481 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    42541 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    42601 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    42661 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    42721 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    42781 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    42841 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    42901 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    42961 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    43021 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    43081 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    43141 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    43201 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    43261 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    43321 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    43381 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    43441 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    43501 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    43561 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    43621 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    43681 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    43741 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    43801 cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt
    43861 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
    43921 cgcaccaaaa accacgttga tattgctcgc actgggcgaa ggtggcggaa ttggtagacg
    43981 cgctagcttc aggtgttagt gttcttacgg acgtgggggt tcaagtcccc cccctcgcac
    44041 caaacgaggc gatatcaaaa aaagtaagat gactgtgcga aggtggcgga attggtagac
    44101 gcgctagctt caggtgttag tgtccttacg gacgtggggg ttcaagtccc ccccctcgca
    44161 ccaattatct ttacttcctt tcttgtttct tccctgtttt gtcatccctg attcaaattc
    44221 attatcatca gcatgatgcc agcaataagc gccaggcacg atggtagcaa aatgtaatgc
    44281 cgcaactgtt tgtgatacag catcaccggc gtcaatacta atcccatcag aatgacaatc
    44341 cgccactgat ccggcgataa atccgcatac atcagccctg caatcagcaa tcccggcagc
    44401 aacactcccc agccactgcc cagcgtacgt tgcaacatgg tttcatctct ttcattgata
    44461 atgaaaacca atatcatatg ataattttta tcatttgcaa gccagagaaa tcccttgcta
    44521 tcgagtaaac ctatcgctag gattagcaat cattatcatt tagattacta tcacgattat
    44581 ggcctatcgt tccgcaccgc tctatgaaga tgtcatctgg cgaacgcatc tccagccgca
    44641 ggatgctggg cttgcacaag ccgtacgggc gatgatcgcc aaacatcgtg aacatttgct
    44701 ggagtttatc cgcctggatg aacccgctcc gcttaacgcc atgacgctgg cgcaatggtc
    44761 atcaccgaat gcgctaagtt ctctactggc ggtctattcc gatcatatct atcgcaacca
    44821 accaacgatg atccgcgaga acaaaccgct gatctcacta tgggcacaat ggtatatcgg
    44881 cctgatggtg ccaccattaa tgctggcgct actgacgcag gaaaaagcat tagatgtgtc
    44941 gccggaacat ttccatgctg agtttcacga aaccggacgc gtcgcctgtt tctgggtcga
    45001 tgtgtgtgaa gataaaaacg caacaccaca ttcgccgcag cagcgaatgg aaacgttaat
    45061 cagccaggcg ctggttccgg ttgtgcaagc actagaagcg accggagaga tcaacggcaa
    45121 acttatctgg agtaataccg gttatttgat caactggtat ctcactgaga tgaagcaact
    45181 gctcggcgag gctactgttg aatcgctgcg ccatgccctc ttttttgaga aaacgctcac
    45241 gaacggtgaa gataatccac tctggcgtac cgtggtactg cgcgacggcc tgctggtgcg
    45301 ccgcacttgc tgccagcgtt atcgcttacc ggatgtgcag caatgtggct attgtacgct
    45361 gaaatgaaag cactgccggg gaagtaaacc cggcatcatg cggattaata gggatgcaac
    45421 acattacttg cttcgcaatg ccgcatacat aacagaatat ctgcggcctc caacaaccct
    45481 gcatcagaac tgacgcccag tttcgacatc acattaaatt tatgcgctcg gatcgttttg
    45541 atattacgtt taagctgctc ggcaatttgt gtcattgagt agccacgcga cataaagcgc
    45601 aatatttctc tctccgtcgg gcttaacgtc cggctttggt taatgtacca ctgattgttc
    45661 agtcggtcgg tcgcctgacg tacaccattt aatgacaaaa agagttcctg atgaaaaatc
    45721 tccagcgttg acgctttact taatacaccg tccagcggtg atggcgacaa tgaaccaatc
    45781 agccgagctt caatatcatc atccacaata actaaacgcc gggtacgcgg aaacttaatc
    45841 gccagctcag tcaggcaaga taatccctct ctgcgctcac ttctcatggc agaaaaagaa
    45901 aaaatgaccg ccgcaaacga gatccgcgac atggcatcct gaaatgactc ctgactggta
    45961 taggtatgga gcttataagg gttgcccgca aactttctgg ataaactctc aataccgata
    46021 ctactcatga cgcatttttc tatgagtgcg acatttctct tcttaattcg gctgtgttcc
    46081 attctgcggc atctccataa acagaaggca agttaagttc tcttgcaccc tgcgcgctat
    46141 ttatccatgc atacatttca gcgttgctgt gaattgccag tcgacgcatc gcactgtttt
    46201 tttgggcgct gatagtttta ttacttttct taagcaatga agctatctgg ttgatgcccc
    46261 atcctttacc taatagccgc agtaccttgc gctctgaaag agtgagaatg acggagcgat
    46321 agctaaaatc atgagtttca gtcattgccg gagaggtcag catggcgctg atacgctctg
    46381 cgtgcgtatt gccggaacgt acggtcttga ccagattctc aatgggttca acatcagaca
    46441 ataacgtcgc cgtagggcac atgagcagtt ctacagcctg ggaataccag gagcgtgaca
    46501 ccatgaaaac ccagtgaatt tcccgatatt gtgagattaa agaataataa tgttcacaaa
    46561 cagaacgtgg atcttcactt tcaccggcta aatcag
//
LOCUS       DAJTNV010000025        45794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNV010000025 DAJTNV010000000
VERSION     DAJTNV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            114..2267
                     /locus_tag="OO199_001382"
     CDS             114..2267
                     /locus_tag="OO199_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5535481.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO199_001383"
     CDS             2320..4050
                     /locus_tag="OO199_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535482.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO199_001384"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO199_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5535483.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO199_001385"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO199_001385"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5535484.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO199_001386"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO199_001386"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5535485.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO199_001387"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO199_001387"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5535486.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO199_001388"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO199_001388"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5535487.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO199_001389"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO199_001389"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5535488.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO199_001390"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO199_001390"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5535489.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO199_001391"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO199_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5535490.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO199_001392"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO199_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5535491.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO199_001393"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO199_001393"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5535492.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO199_001394"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO199_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5535493.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO199_001395"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO199_001395"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5535494.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO199_001396"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO199_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5535495.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO199_001397"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO199_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5535496.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16836
                     /locus_tag="OO199_001398"
                     /pseudo
     CDS             16308..16836
                     /locus_tag="OO199_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            17134..18018
                     /gene="tsx"
                     /locus_tag="OO199_001399"
     CDS             17134..18018
                     /gene="tsx"
                     /locus_tag="OO199_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5535497.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18195..18542)
                     /locus_tag="OO199_001400"
     CDS             complement(18195..18542)
                     /locus_tag="OO199_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5535498.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18671..19642)
                     /gene="secF"
                     /locus_tag="OO199_001401"
     CDS             complement(18671..19642)
                     /gene="secF"
                     /locus_tag="OO199_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5535499.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19653..21500)
                     /gene="secD"
                     /locus_tag="OO199_001402"
     CDS             complement(19653..21500)
                     /gene="secD"
                     /locus_tag="OO199_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5535500.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21528..21860)
                     /gene="yajC"
                     /locus_tag="OO199_001403"
     CDS             complement(21528..21860)
                     /gene="yajC"
                     /locus_tag="OO199_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5535501.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21883..23010)
                     /gene="tgt"
                     /locus_tag="OO199_001404"
     CDS             complement(21883..23010)
                     /gene="tgt"
                     /locus_tag="OO199_001404"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5535502.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23065..24135)
                     /gene="queA"
                     /locus_tag="OO199_001405"
     CDS             complement(23065..24135)
                     /gene="queA"
                     /locus_tag="OO199_001405"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5535503.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24228..24801
                     /gene="acpH"
                     /locus_tag="OO199_001406"
                     /pseudo
     CDS             24228..24801
                     /gene="acpH"
                     /locus_tag="OO199_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24806..26623)
                     /gene="malZ"
                     /locus_tag="OO199_001407"
     CDS             complement(24806..26623)
                     /gene="malZ"
                     /locus_tag="OO199_001407"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5535504.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26779..28152)
                     /gene="proY"
                     /locus_tag="OO199_001408"
     CDS             complement(26779..28152)
                     /gene="proY"
                     /locus_tag="OO199_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5535505.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28228..29547)
                     /gene="brnQ"
                     /locus_tag="OO199_001409"
     CDS             complement(28228..29547)
                     /gene="brnQ"
                     /locus_tag="OO199_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5535506.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29954..31249)
                     /gene="phoR"
                     /locus_tag="OO199_001410"
     CDS             complement(29954..31249)
                     /gene="phoR"
                     /locus_tag="OO199_001410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5535507.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31307..31996)
                     /gene="phoB"
                     /locus_tag="OO199_001411"
                     /pseudo
     CDS             complement(31307..31996)
                     /gene="phoB"
                     /locus_tag="OO199_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32186..33388
                     /gene="sbcD"
                     /locus_tag="OO199_001412"
     CDS             32186..33388
                     /gene="sbcD"
                     /locus_tag="OO199_001412"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5535508.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33385..36528
                     /gene="sbcC"
                     /locus_tag="OO199_001413"
     CDS             33385..36528
                     /gene="sbcC"
                     /locus_tag="OO199_001413"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5535509.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36654..37835
                     /gene="araJ"
                     /locus_tag="OO199_001414"
     CDS             36654..37835
                     /gene="araJ"
                     /locus_tag="OO199_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5535510.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37978..38886)
                     /gene="mak"
                     /locus_tag="OO199_001415"
     CDS             complement(37978..38886)
                     /gene="mak"
                     /locus_tag="OO199_001415"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5535511.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39011..39922
                     /gene="rdgC"
                     /locus_tag="OO199_001416"
     CDS             39011..39922
                     /gene="rdgC"
                     /locus_tag="OO199_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5535512.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40000..40455)
                     /locus_tag="OO199_001417"
     CDS             complement(40000..40455)
                     /locus_tag="OO199_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535513.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40452..40787)
                     /locus_tag="OO199_001418"
                     /pseudo
     CDS             complement(40452..40787)
                     /locus_tag="OO199_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40942..41226)
                     /gene="ppnP"
                     /locus_tag="OO199_001419"
     CDS             complement(40942..41226)
                     /gene="ppnP"
                     /locus_tag="OO199_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5535514.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41298..41975)
                     /locus_tag="OO199_001420"
     CDS             complement(41298..41975)
                     /locus_tag="OO199_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5535515.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42236..42424)
                     /gene="yaiA"
                     /locus_tag="OO199_001421"
     CDS             complement(42236..42424)
                     /gene="yaiA"
                     /locus_tag="OO199_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5535516.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42474..42998)
                     /gene="aroL"
                     /locus_tag="OO199_001422"
     CDS             complement(42474..42998)
                     /gene="aroL"
                     /locus_tag="OO199_001422"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5535517.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43181..43639)
                     /locus_tag="OO199_001423"
     CDS             complement(43181..43639)
                     /locus_tag="OO199_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5535518.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43759..44568
                     /gene="proC"
                     /locus_tag="OO199_001424"
     CDS             43759..44568
                     /gene="proC"
                     /locus_tag="OO199_001424"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5535519.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44585..45700)
                     /gene="adrA"
                     /locus_tag="OO199_001425"
                     /gene_synonym="dgcC"
     CDS             complement(44585..45700)
                     /gene="adrA"
                     /locus_tag="OO199_001425"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5535520.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11906 a        11939 c        11201 g        10748 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact catgaatgca aaatccagca gtggggttta ccttctgcct
    16501 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    16561 aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg tgagtcgaat
    16621 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    16681 aattttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc
    16741 cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca gttaggcttt
    16801 gtacgtatcc acgacattca acctgttatg cagtaaacgt atttcgggac gattttgtgc
    16861 gtcccgcaac atcttccccg tcattttgtt actctgctta catcacctgg attgatagta
    16921 aaagtttgca acaagggcga aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa
    16981 cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac atatttttgt gagcaatgat
    17041 ttttataata ggctcctctg tatacgaaat atttagaaac gcaatttgcg cctttttcac
    17101 tcccgcaagg gattttcaaa cagtggcata catatgaaaa aaacattact ggcagccggt
    17161 gcggtactgg cgctctcttc gtcttttact gtcaacgcag ctgaaaacga caaaccgcag
    17221 tatctttccg actggtggca ccagagcgtt aacgttgtcg gaagctatca cacccgtttc
    17281 ggaccgcaga tccgcaacga tacctacctt gagtacgaag cattcgctaa aaaagactgg
    17341 ttcgacttct atggttatgc ggatgcgccg gtattcttcg gcggtaactc cgatgctaaa
    17401 ggtatctgga accacggttc tccgctgttt atggaaatcg aaccacgttt ctccatcgac
    17461 aagctgacca atactgacct tagcttcggt ccgttcaaag agtggtactt cgcgaacaac
    17521 tacatttacg acatgggtcg taataaagat ggtcgccaga gcacctggta catgggtctg
    17581 ggtaccgata tcgacactgg cctgccgatg agcctgtcca tgaacgtcta tgcgaaatac
    17641 cagtggcaga actatggcgc agcgaacgaa aacgagtggg acggttaccg tttcaaaatt
    17701 aaatactttg tgccgattac cgatctgtgg ggcggtcagc tgagctacat cggcttcacc
    17761 aacttcgact ggggttccga tttaggggat gacagcggta acgcaatcaa cggtattaag
    17821 acccgtacta ataactctat cgcttccagc catattctgg ctctgaacta cgatcactgg
    17881 cactactctg tcgtagctcg ttactggcac gacggtggtc agtggaacga cgatgcagaa
    17941 ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg gctggggtgg ttacctggta
    18001 gtaggttaca acttctgatt atgaaaatgc cgggatttat tcccggcatt tctgattgtt
    18061 aacaacgtgt gctttgttca tgccggatgc ggcgtaaacg ctttgtccgg cccacaaaag
    18121 cacgtaaatt caatatattg ctgaaataac gtaggcctga taagcgtagc gcatcaggca
    18181 attttacgtt ttaatcactt cttcttgttc atcatcgctt tcaggtcagc gaatgggttg
    18241 tacttcgctt caccgacatc tttctgcgca tcttcccctg cgataacggt cgtaccatac
    18301 tgatccgctt cggtatattt cgaatgctca tgatcgtggc aatagagaca caacaattcc
    18361 cagttactgc catcttccgg gttattggta tggtcgtgat caatgtggtg aacggtaagt
    18421 tcacgcaggt tagaataaac aaactcgcgg gaacagcgac cgcagaccca cggatagatt
    18481 tttaatgctt tttcgcgata gccgctttct aaccgtgcat agttttttgg gatgatagcc
    18541 atgaaaagtt acctggatga aatgtagggt tacgcgcagt ttcccaagat gcggggattt
    18601 tcgcaatctc catacatcaa aaaaaatccc gatcttctga ccgggatttt caacatcaac
    18661 gggaacttga ttaaggcaga attgacggct gatccgcccc ttctttttcc actttctgct
    18721 gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa cgcagatgct acgtagatgg
    18781 aagatgcagt gccgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg
    18841 gaccaccgaa gagatacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg
    18901 tacggtgcag cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga
    18961 tcttgcggaa gttttcacga attcggtccg ataccacgat actgtcgtta agcgagtaac
    19021 cgataaccga catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca
    19081 aaatacccag cgtaatgata acgtcgtgcg tcagcgcaat aaccacccct gccgccagtc
    19141 gccactcaaa gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca
    19201 tcgcaccggt ttgcgcaagg tcagccccca cgctcggacc gacgaactca atacgcttca
    19261 ctgctgcatt ctgattggtg gattcgttaa tcaccttcag aacttggctg cccagcacct
    19321 gaccgccggt ttcgccttca gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa
    19381 agttttgcag cattggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa
    19441 tttcagccgg tttttcgagc gtaatttcga taaccgtacc accggtgaaa tccagccccc
    19501 agttaaagcc gcgcacgccc ataataacga tagcagcgat taacagcaga ccagagatgc
    19561 cgaaagccca gtagtcccag cgcataaagt catagacttt acggccgtgg tttagttgtt
    19621 caacagtata ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg
    19681 ccgccatata gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc
    19741 gccacaccga taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac
    19801 aggatgatga ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca
    19861 cgataacctt catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata
    19921 cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac gatacccgcg
    19981 atacctggca tactcagtgt tgcgcctggc aacagcgaca taataccgac gatcaagatc
    20041 aagttggcaa tcagagcact ggtcgcaatc agaccaaact tcttatagaa gatgatcatg
    20101 aacagaatag aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc
    20161 atacccaagg tcgggccaat tgtacgttct tcaacaatct gaatcggcgc gatcaacgca
    20221 ccggcacgca gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata
    20281 cggaagctgt tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc
    20341 accagaaccg cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg
    20401 gttgccatcg gtttgccgat gttgtccttg gtgaagttag acataatgtt gccaccagcg
    20461 ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt cctggctgga agtggagtcg
    20521 gtgatatggt caccagtcag aattacgcgt ttgtacagca caaccggctg accttcgcgg
    20581 gtctgtttca cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctgatcaaca
    20641 ttggtgttta ccagacggaa ttccagcgtt gccgtcgcgc ccagaatctc tttcgcacgc
    20701 gcagtgtcct gaataccggg cagttcaaca acgatacggt cagcaccctg acgctgaacc
    20761 accggctccg ccacgccaag ttggtttaca cggttacgca ggatattaat gttctgctgc
    20821 accgcatatt cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg
    20881 ctgccctggc tgctaatcac caggtccgga tggcgcttgc tcagatacgc aatggcttca
    20941 tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt agttgttttc tttacgaaca
    21001 gtggtatacg ggatgccctt ttcgcgcagg tcactgcgta ggctatcgat attttgttcc
    21061 tggagtttgc caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc
    21121 aggtcaaggc cgagcttcat cggctcagca tgaatagctg ccagccagcg cggcgttgcc
    21181 ggggcaaggt taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga
    21241 gcgcgcaact gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc
    21301 acagacttag cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc
    21361 tcactggcgg cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg
    21421 ttgggaagcg catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac
    21481 aaaggataac ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc
    21541 atggtgcctt tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg
    21601 tcattcagcg cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg
    21661 ccaccgttcg tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct
    21721 ttggtgcgct tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc
    21781 agcatcaaaa tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca
    21841 tcagaaataa aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg
    21901 tggtacttct cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc
    21961 ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt
    22021 gttgagtcgc gcgcctaata tttcgttgca acggtcgaga tgatgcaagt aagcgcgtga
    22081 ataattgcga caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata
    22141 tttcgcattg cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt
    22201 gcgggttggc attacgcagt caaacatatc gataccgcga cgtacgcctt caaccaggtc
    22261 ttccggttta ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcggacacac
    22321 atgctccaga atacggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc
    22381 gtagccatca aagccgatat ctaccagacc tttaacagat atatcacgta aatcttcgta
    22441 aatactgccc tgaattatac caaataacgc atttttgtta ccaagactgt caaaacgctc
    22501 acggctacgc ttcgcccaac gcagagacat ctccatagag cgttttgcgt aatcccagtc
    22561 agcaggatac ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg
    22621 aatctccatc gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg ggttacggaa
    22681 gtgaacgccc tgttcggtga ttttacgaat atcaccaagg ctgaagacct ggaagccgcc
    22741 ggagtcggta agaatcggtc ctttccactg cataaaatcg tgcagatcgc cgtgcagttt
    22801 catgatttcc tggcccgggc gcagccacag gtggaaggtg ttgccgagga taatttgcgc
    22861 gccagtggct tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg
    22921 cataaaacaa ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg
    22981 accgtcggtg gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc
    23041 aacgtttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt
    23101 gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgccgc
    23161 tttataggca ttcatggtgt gttgataacc ggcaaaggcc gaaaccagca taatcagcgt
    23221 cgactctggc aagtggaagt tcgtcaccag cgcatcgacc actttgtact ggaagcccgg
    23281 atagataaag atttgggtat cgtcaaagaa cggttcgata agatcgtttt ttgccgcctg
    23341 tgccgcgctt tccagcgaac gtactgaagt ggtgccaacc gcaatcaccc ggttaccgcg
    23401 cgctttcgcc gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg
    23461 catgatgtga tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg caccaacgtg
    23521 caacgtcaca aacgccatct ccacgccttt ggcgcgtaat ttttccagca aaggctcgtc
    23581 aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg
    23641 ataaagttcg cggtcagcgt cttcgtccgg acgatcgata tacggcggca gcggcatatg
    23701 gccgatgctg ttgaggatat ccagtaccga gcgttcatca ttaaattcga cttcaaacag
    23761 tgcgccgtgg cgcgcggtca ttgttgcgtt gatactttcg tcatcgccca gcaacagttc
    23821 tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag
    23881 catccgctca accagcactt caatcttgcc gccgctggct ttacgcccaa acagacgcgc
    23941 cgggatcacg cgggtattat taaaaaccag aagatcgccg gggttgagct tatcgagtaa
    24001 atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca gtaaacgaca
    24061 gctactgcgt tcaggcatgg gatagtgggc aatcagggat tcgggcaatt caaaggagaa
    24121 atcggtaacg cgcatgacac tgactcagac taaaataaga gtcgggtagt ctagtgccgg
    24181 ggcgctctcc ctgcaacctt tacccccctt tggatagaat ataatcgatg aattttttag
    24241 ctcacctgca tttagcccat ctcgcggaaa gctcgctttc tggcaattta ctggctgatt
    24301 tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc
    24361 atcgacgtat tgactgacaa tctgccggaa gtccgcgaag cacaggagtg gtttcgtagt
    24421 gaaacgcgcc gcgttgcgcc tattacgctg gatgtcatgt gggatcactt tctttcccgc
    24481 cactggtcgc agctgtcgcc ggactttccg ctacaggaat ttgtctgtta tgcccgcgag
    24541 caagtgatga cgattttgcc ggactcaccg ccacgtttta tcaatctgaa caattacttg
    24601 tggtcagagc agtggctggt gcgctatcgc gatatggatt tcatccagaa cgtgttaaac
    24661 ggcatggcaa gccgccgccc acgtctggat gccctgcgtg actcctggta cgatttagac
    24721 gctcattatg acgccctcga aacccgcttc tggcagtttt atccgcggat gatggcgcag
    24781 gcgtcacaca aggcgttata aaacgttagt tcatccatac cgtagccgaa atggcaggca
    24841 aagccagaat cccgtcagtc agttgcccat gcccttcttt gcattgccat tgcacggcat
    24901 tgagaaacgg tgacgcgggt agcaccactt cactggcctc gccacggttg attgccacca
    24961 gtacacgttg ctgattcagc acgcggacaa ataccactac gttatcttcc gcatacagca
    25021 cctgacagcc gccacgacgc agcgcctgac ttttcttacg cagcacaatc attcgccggt
    25081 acagcgcgaa taacgccgta tcctgctttt ccacctgcca ggggaacggt ttacggcaaa
    25141 acggatcgtt tttaccatcc agtcccactt catcaccgta ataaatgcac ggtacaccag
    25201 gccaggtgaa cagccagacc accgccagcg gcaggcgcgc aatatcccga ccgagcagcg
    25261 ttttaaatcg cgcagtatcg tggctatcga gctgattaaa catacgtaat tgttgttgat
    25321 gagaaagccc tgcgcggtaa ttatccatcc aggccataca ggtttgggcg tcaatttgct
    25381 gcggatcata agagatgtcg gtattggcaa gaaatcccca caacgggaat gtgaagccac
    25441 gatagttcat ggcagcatct tccacatcgg cctgtaacca ttgccgtgca tcgccaaaat
    25501 gttcgccgac aatatacgct tccggctggg tttctttcgc cgcttcggtg atcccggcaa
    25561 cgtgctgcat attattgcgc gccccacccg cctcccccag catatgcacc acatccagcc
    25621 gccagccgtc catattccac ggcgctttca gccagtggcg gacaatactg tcttccccgc
    25681 gataaatttc attcaccaga ctttccgact gataatccag cttcgtcaag ctggcatagc
    25741 caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc cagggcgatt
    25801 cagagttgtg acaagctccc cccgtgccaa gattgtgcct gtcaaaccag gcatgggaat
    25861 cgccactgtg gttaaacacg ccgtccagca ccagccgcat tcccagctgc tgcgtattgt
    25921 gtcgcaaacg cagcaacacc ccatcaccgc caaactgcgg atcgacatgg cgataatcct
    25981 cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac agcgctgtca
    26041 cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg ccgccataga
    26101 acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag atgatctctt
    26161 gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga agactacgcg
    26221 caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt gggccgatat
    26281 caggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg aagccctgcg
    26341 gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt
    26401 gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gcctggctgc ggctgactgc
    26461 gctgcttatg cattggtact gacatttctt cgttatcgtg ttctgtacgc agcataatgc
    26521 gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct ttgctttgtt
    26581 taacaaatgg gggcaccggc aggtgccatg catttaacat cataattccc ctgtgatgaa
    26641 aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag gatatttggg
    26701 ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc tcctgaaccg
    26761 gcttacagaa cgcaagtatt attggttttc agccagctga cgatcgtggc ggcgtttaaa
    26821 catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg agatacgcgt
    26881 atccgggtga taaccaatca acccgataat aaagagcagg aaaatcagcc cgccgatggt
    26941 cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg gcggcaaacg
    27001 gcgacggaag gcgatttgcg acagcaggat cataatccac acccataccg tggcgaaggt
    27061 tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca gatacaccgc
    27121 aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc gacgcgacgt
    27181 tttgctgaaa attttcggtg cgctgccctg ctctgccata ccgtggagca tacggcctac
    27241 gccaaatacg tcactgttaa ttgccgacag cgaagcggtc agcacaacaa agttaagaat
    27301 gctggcggca aaggtaatgc ccatatgctg gaacgtcagt acgaacgggc taccggcagt
    27361 gccaacctga ttccacgggt agatagacat aatgacgaac agcgtaccga cgtagaacac
    27421 cagaatacgc atcggcacgg agttaatcgc acgcggaatc gatttctcag gatctttcgc
    27481 ttcaccggcg gtaatcccga taatttcgat cccaccgtaa gcaaacatca ccatttgcaa
    27541 cgacattacc atgccaagcc agccgttact gaagaagccg ccgttgctcc acaggttatg
    27601 aataccggtc ggttgcccgc cgttgccaat cccccagatg atgatgccga aaccggcgac
    27661 aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca gctcaccgaa
    27721 gaccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca gtacccaaat
    27781 ccagtgcggc accgtcggga acaagacacc catatagata ccaaaagcgg tcacatcggc
    27841 gatggcgaca ataaggattt caaaacagta ggtccatccg gtaatgtaac ctgccagcgg
    27901 gccaaggttt tcctgcgcat aacgcgagaa agagctggcg gccgggttat gtaccgacat
    27961 ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat aggccaacaa
    28021 cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat
    28081 tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct ttagcttatt
    28141 cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg catcgcagcc
    28201 ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct gacgacctgc
    28261 cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta accacgccag
    28321 accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga
    28381 tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg gagcaatcac
    28441 gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg cgatacacgg
    28501 cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta agccgaggtt
    28561 agacaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag agagcggtac
    28621 gtactgggca aagaattctg cacaagcaca ggtcaggcca accgccgtga ccagacaggc
    28681 gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt gctgaacgta
    28741 agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc
    28801 cagacagaac agcgccaggt acagcaaagt cagaccaaca cccgccatca ggccagccca
    28861 gacggtataa cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac
    28921 aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc cgttaacgaa
    28981 gccgttagaa aatgcagcgt tttgataagc ctcagtcgcc gtgctgatag aacccgccgg
    29041 ccagacaatc gcgacaacag acaggatgac cagcgcgata attttcagcg gcgcaaggaa
    29101 gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa cgatagcgaa
    29161 atagaccagg ctgtagataa acagcggcag cgcggaatca cccgtcagcg gcgcaatccc
    29221 cacttcaaag gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta
    29281 acaaaccgtt gccagcagta cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac
    29341 accgccgcca acttttgcca gcgccactac cgttaatacc ggcaggccaa cggcagtaat
    29401 gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga ccattggagg
    29461 gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat
    29521 atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac
    29581 gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac aattttagcg
    29641 ggaaatactc ccaaccaggc gcggcgaaga atagtttttc gatttaccgc gttaatgcag
    29701 tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa gcaaggaggg
    29761 atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa acactatttc
    29821 cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac
    29881 gcccgtattt tgactgcaaa gaaaataagc cagcatcggc tggcttatgg caataaaaga
    29941 tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata acaaaactga
    30001 aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga tgattcaccg
    30061 catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa cgcgctttat
    30121 caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt
    30181 tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg
    30241 tgccttccgg cgtatgattc acggcgttat agaccaggtt cgaaatcgca ctgcgtagct
    30301 gatcttcgtt gccagacacc ttgaggccgt tatctatctc aaaggtaaat gtctgttttt
    30361 tctgactcag agtctgagcc tcgcgctcaa caacgcgcag catcatcggc acatcaacct
    30421 tttcattgag caattgcgtc ggcgcggctt caattttcga cagcgttagc aactgcttca
    30481 ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg
    30541 cgccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg
    30601 tacgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc agttgatgca
    30661 tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc atgacgcgaa
    30721 tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt
    30781 tcagatattg cgtaaactcc gggtaacgca gtaggttaag gatgttctgc ccgttatctt
    30841 ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa ataccgccct
    30901 cttccgtggt cagcaccacc gcgtcgggca gcgactccgc gccgctacga aagcgtttaa
    30961 tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt
    31021 atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactgcga tccacccaca
    31081 gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtcct gttaccgatg
    31141 ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc gggaggcagc
    31201 aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg taagatactc
    31261 cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg
    31321 aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg ccgggctcca
    31381 gtgctttacg caggcgacga atgtggacat cgaccgtgcg gtcttccaca tacacgttag
    31441 ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga tgcgtcataa
    31501 aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg cccgccatca
    31561 ctcggtgaga tgtcgggtcg agacttaatc cctgcatctt aatcacctct tccaccgcca
    31621 ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc ggcgaaaatg
    31681 gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct tcttcttccc
    31741 ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc ttgaggtgtt
    31801 tgatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt aaatccggct
    31861 agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc ggctgaaagc
    31921 cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct tctacgacca
    31981 gaatacgtct cgccatgatt tgccctgttg taataaatag gttgcgatca ttaatgcgac
    32041 gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat atcttcaggt
    32101 tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta taatcgcgac
    32161 catgcttttt cgccagggaa ccgttatgcg catccttcac acctcagact ggcatctcgg
    32221 ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg actggctgct
    32281 ggagacagca caaacccatc agatggatgc gattattgtt gctggtgatg ttttcgatac
    32341 cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca atttacagca
    32401 aactggctgt catctggtgg tactggcagg aaaccatgac tcggtcgcca cgctgaatga
    32461 atcgcgcgat atcatggcgt tcctcaatac aaccgtggtc gccagcgccg gacatgcgcc
    32521 gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt
    32581 acgcccgcgt gacattattt ccagccaggc ggggcttaac ggtattgaaa aacagcagca
    32641 tttactggca gcgattaccg attattacca acaacactat gccgatgcct gcaaactgcg
    32701 cggcgatcag cctctgccca tcatcgccac gggacattta acgaccgtgg gttccagtaa
    32761 aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccgg cacaaaactt
    32821 tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta ttggcggcat
    32881 ggaacatgtt cgctattgcg gttcccccat tccactgagt tttgatgaat gcggtaagag
    32941 taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa
    33001 cgtaccggta actcaaccca tggcggtgct gaaaggcgat ctggcgtcga ttaccgcaca
    33061 gctggaacag tggcgcgatg tatcgcagga gccacctgtc tggctggata tcgaaatcac
    33121 tactgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg aatcattgcc
    33181 tgtcgaagta ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt tagccagcca
    33241 acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact
    33301 ggaagaactg gatgaatcgc agcagcaacg tctgcagcat cttttcacca cgacgttgcg
    33361 taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac
    33421 tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag caacgggctg
    33481 tttgccatta ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc catttgtctg
    33541 gcgctgtatc acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg
    33601 acccgcgata ccgctgaatg tctggcggag gtggagtttg aagtgaaagg tgaagcgtat
    33661 cgcgcgttct ggagccagaa tcgggcgcgt aaccagccgg acggtaattt gcaggtgcct
    33721 cgcgtagagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt gaaagataag
    33781 ctggaactga cagcgacgtt aaccgggctg gattacgggc gcttcacccg ttcgatgctg
    33841 ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg cgcggaattg
    33901 ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt ttttgagcag
    33961 cacaaatcgg cccgcactga gctggagaag ctgcaagcgc aggccagcgg cgtcacgttg
    34021 ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac tgacgaagaa
    34081 aaacagttaa ttaccgcgca gcagcaagaa caacaatcgc taaactggtt aacgcgtcag
    34141 gacgaattgc agcaagaagc cagccgccgt cagcaggcct tgcaacaggc gttagccgaa
    34201 gaagaaaaag cgcaacctca actggcggcg cttagtctgg cacaaccggc acgaaatctt
    34261 cgtccacact gggaacgcat cgcagaacac agcgcggcgc tggcgcatat tcgccagcag
    34321 attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag cattcgccac
    34381 cacgcggcga agcagtcagc agaattacag cagcagcaac aaagcctgaa tacctggtta
    34441 caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg tgcgcagttc
    34501 tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt aacccatgct
    34561 gagcaaaaac ttaatgcgct tgcggcgatc acgttgacgt taaccgccga tgaagttgct
    34621 accgccctgg cgcaacatgc cgagcaacgc ccactgcgtc agcgcctggt cgcgctgcat
    34681 gggcagattg ttccccaaca aaaacgtctg gcgcagttaa tcgtcactat ccagaatgtc
    34741 actctggaac agacgcaacg taatgtcgca cttaacgaaa tgcgccagcg ttataaagaa
    34801 aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg catcaaaacg
    34861 ctggaagccc agcgtgcaca gttacaggcg ggtcagcctt gtccactttg tggttccacc
    34921 agccacccgg cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca gtctcgatta
    34981 ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa
    35041 ctggatgcat taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa
    35101 gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg
    35161 cagccacagg acgatattca accgtggctg gatgcacaag atgagcacga acgccagctg
    35221 cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa tcagcaaatt
    35281 atccagtatc aacagcaaat tgaacaacgt cagcagcagc tcttaacggc attggcgggt
    35341 tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac acgtcagcaa
    35401 gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa ccgtattcag
    35461 cagctgacac cgattctgga aacgttgccg caaagtgatg atctcccgca cagcgaagaa
    35521 actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt acacagccag
    35581 cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa agcccaagcg
    35641 cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg
    35701 ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct ggaaaaccag
    35761 cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca gcatcaacaa
    35821 caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca agagttagcg
    35881 caaactcacc aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg ccagcagctg
    35941 aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat tgctcaaatg
    36001 acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa agagggcgat
    36061 aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct cgctaatcag
    36121 caacttaccc ggctgcacgg gcgctatctg ttacagcgca aagccagcga ggcgctggaa
    36181 gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac cctttccggc
    36241 ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct ggtcagccat
    36301 aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga tagcgaaacg
    36361 ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg
    36421 attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt gaaaaagatc
    36481 aacggcctgg gctacagcaa actggaaagt acgtttgcag tgaaataact attcagcagg
    36541 ataatgaata cagaggggtg aattatctct tggccttgct ggtcgttatc ctgcaagcta
    36601 tcactttatt ggctacggtg attggtagcc gttctggtgg ttgtgatggt ggtatgaaaa
    36661 aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa tttggcatta
    36721 tgagcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct gccgggcata
    36781 tgatctcgta ttatgcactg gtggtggtgg tcggtgcgcc aatcatcgca ctcttttcca
    36841 gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc attggcaacg
    36901 ccatgttcac gctctcttcg tcttacctga tgctcgccat tggtcggctg gtatccggct
    36961 ttccgcatgg cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg
    37021 gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc aatttgctgg
    37081 gcattccgct gggaacgtat ttaagtcagg aatgctggcg ttacaccttt ttattgatcg
    37141 ctgtttttaa tattgcggtg atggcatcgg tctatttttg ggtgccagat attcgcgacg
    37201 aggcgaaagg aaatctgcgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa
    37261 ttttcgccgc cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc
    37321 catacatgat gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag
    37381 ttgggctagg gatggtgctg ggaaatatgc taagtggcag gatttcagga cgttattcac
    37441 cactgcgcat tgcagcagtg actgacttta tcattgtact ggcactgctg atgctctttt
    37501 tctgcggcgg catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat
    37561 ttgccctttc agcacctctg caaatattgt tactgcaaaa tgccaaagga ggagagttat
    37621 taggtggcgc aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg
    37681 gaggtatgat gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgc
    37741 tttcgtttgc tgcgatgtca tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg
    37801 cggatagtcc ggtgctggcg aaaccactgg ggtagattac tgtcacggtg cgcgttgctt
    37861 attgccggat gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgtagg
    37921 cctgataaga cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct acgtttttta
    37981 ctcttgtggc cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt
    38041 actcaccggc gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt
    38101 ttgatataaa cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag
    38161 aatattcacg acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag
    38221 ttccgctacc ggatcgcttt cttcaaccag gcggataatt tcactgcctt tcagcgcatg
    38281 tccgctcaaa cgacgataat ccgtcgcgaa tcccgtgccc gaaataaagg tttcaataca
    38341 accttgttta ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat
    38401 ccacggtagc ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgtccg
    38461 cccattgaat gccacgcccg cgccgcatcc cgtgccgata gtcacggcaa ataccgtctg
    38521 cgctcccgct gccgcgccat ctactgcttc tgaaaccgcc agacagttgg catcatttgc
    38581 cagccgcact tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag
    38641 ccaggttgaa ttgacattct tcaccacacc ggtgtagggc gaaatcgaac ctggaatgcc
    38701 catacctacc gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat
    38761 cgtttcaata gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg
    38821 ctcccctgca tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat
    38881 acgcactgta ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt
    38941 ggcaatgcaa ggccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt
    39001 ggaaattatc atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc
    39061 gctgcgtgca gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag
    39121 ccaggacatg gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac
    39181 gcacgttgcc aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc
    39241 tccggtgatt aaacaggcgc tggaagcgaa aatcgccaaa ctggaagcgg aacaggcgcg
    39301 taagctgaag aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc
    39361 gcgtgctttc agccgtttta gccagacaat gatgtggctc gacacggtta acggtttgat
    39421 tatggtggac tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag
    39481 cctggggtcg ttaccggttg taccattgag catggaaaac ccgattgaac tgacgctgac
    39541 cgaatgggtt cgctccggta gtgcggcaca gggcttccag ctgcttgatg aagccgagct
    39601 gaaatcgttg ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga
    39661 agagatcacc aatcacattg aagccggaaa agtggtgact aaactggcgc tcgactggca
    39721 gcagcgcatt cagtttgtga tgtgcgacga tggttcgctc aagcgtctta agttctgcga
    39781 cgagctgcgc gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc
    39841 cgatttcatc ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt
    39901 aggtggcgaa gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc
    39961 gcgtcttatc cggcgtacga agccacacca agcatataat tattcgctac ggcgagcaat
    40021 aatttttagc gcagcaatat tatgcgtttt acgccgtaac tgatctaatc gcctcatccc
    40081 tattcgcctg tcagcagcac gcagaatatt cactaacccc catttatcgc tggtaaattg
    40141 ttttgttaac agctccgctt ttggggtatc aatatattca cgcaataaat cagaaattga
    40201 acaaatatcc gtcaggtaag gataccagca agtttcaccg tgaaaattac gcactccagc
    40261 gttacctgca ataaagtctt tcgggtaatc ccagatcacg tttttatcca acgtgatcca
    40321 ataacgcggt aaatcggtac ttccaccatt ttggctgcgc atgggataac gggtacagtg
    40381 aatttgcagg ttgatcgttg gcgtcagtaa gtcatagatc tcccgctgta acttgctcca
    40441 tggacgttgt gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg aattttccgc
    40501 gttgccccgg ttttcactaa atcacgtaac tgcgaaagat cgggttattc gacaaataag
    40561 agtaatgatg gatcctcttt taaccacgcc gttttcacat cggcagccag ctgtggatta
    40621 ttaatagcca gcgaccgatc ctgaggctca gtcaatgtcg ttaacaacga cgccggggta
    40681 tgtgcgttgc gtaatgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacgc
    40741 caccattcat catctgattt atcttttatc gtagccagtt ccgcaaaaaa tacctggaga
    40801 tcttcactga tatttatatc aaccttgatg aattatgaat atatcggaaa ttgaaaaaat
    40861 cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa atgccctccc
    40921 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtag gttcggcaac
    40981 ttgaagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacttg
    41041 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
    41101 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
    41161 agtgctgctg ctggaaaagc cgatcgattt cactttgccg gaaaagtact cattactttg
    41221 aagcataaac tggccctgtc tggtagttga acatctactt cactataggg gccagaggcg
    41281 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
    41341 gagagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
    41401 cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccagcatca
    41461 atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg cgatttctgc
    41521 aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac gataaccccc
    41581 acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga cggctcaaga
    41641 aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa taaaatgacg
    41701 tcataaccct gattatcgag cacttcaacc acaccttgca gatctcgctc cactttacga
    41761 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
    41821 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag gctatgatgg
    41881 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
    41941 atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta cttttctttc
    42001 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
    42061 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
    42121 atggaatgtt attgtttaat attctaatta ttagaaaaac ataaattatg aaaaatgtga
    42181 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactatt
    42241 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagccgtc ggatgttcac
    42301 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
    42361 ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
    42421 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcta aaatcaacaa
    42481 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
    42541 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
    42601 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
    42661 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
    42721 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
    42781 gtggatgccg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
    42841 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
    42901 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
    42961 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
    43021 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat accatcaaac tagtacaatt
    43081 tcgattgtaa agaaaaaatt ccacttaaag taaaaatatc aatacatccc ttactatacc
    43141 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
    43201 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
    43261 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
    43321 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcgaatta agcgccgctg
    43381 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
    43441 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
    43501 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
    43561 tctgcatacg ttccgccgcg agatacaaaa tctctttaat tacattggga cacgcgtcgg
    43621 catccaccca aattgtcata aagtcatcct ttgttaggta atcctctatt gtgtcgcgct
    43681 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
    43741 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
    43801 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
    43861 tacaccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
    43921 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt taaacctggc
    43981 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
    44041 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
    44101 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
    44161 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
    44221 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
    44281 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
    44341 atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
    44401 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
    44461 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
    44521 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
    44581 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
    44641 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
    44701 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg tttagccctt
    44761 cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattactg
    44821 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
    44881 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta tcgttgatgc
    44941 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
    45001 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
    45061 cgtcgcgagt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
    45121 ggtagctgac ccagccaaac atcagaggat aaacgacaat aatgggaagg gagagccacc
    45181 attccagcgg agcactattg aacgacacgg taatgcccgt aagctcgagg gtgacaaggc
    45241 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
    45301 aattcagaca cagaatcatc aacatagcgg tggaaggcag cacgtttacg cccattacgc
    45361 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
    45421 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
    45481 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
    45541 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
    45601 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
    45661 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
    45721 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact ttcgtagctc
    45781 acccgttagc acta
//
LOCUS       DAJTNV010000026        43470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNV010000026 DAJTNV010000000
VERSION     DAJTNV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO199_001426"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO199_001426"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5535521.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO199_001427"
     CDS             complement(1672..2730)
                     /locus_tag="OO199_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5535522.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO199_001428"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO199_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO199_001429"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO199_001429"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5535523.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO199_001430"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO199_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5535524.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO199_001431"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO199_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5535525.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO199_001432"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO199_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5535526.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO199_001433"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO199_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5535527.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO199_001434"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO199_001434"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5535528.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO199_001435"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO199_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5535529.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO199_001436"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO199_001436"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5535530.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO199_001437"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO199_001437"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5535531.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO199_001438"
     CDS             complement(17699..18322)
                     /locus_tag="OO199_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5535532.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO199_001439"
     CDS             18619..18894
                     /locus_tag="OO199_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535533.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO199_001440"
     CDS             complement(18971..19213)
                     /locus_tag="OO199_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5535534.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO199_001441"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO199_001441"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5535535.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO199_001442"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO199_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5535536.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO199_001443"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO199_001443"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5535537.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO199_001444"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO199_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO199_001445"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO199_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5535538.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEVFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO199_001446"
     CDS             24840..25697
                     /locus_tag="OO199_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5535539.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO199_001447"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO199_001447"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5535540.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO199_001448"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO199_001448"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5535541.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO199_001449"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO199_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO199_001450"
     CDS             complement(30355..31602)
                     /locus_tag="OO199_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5535542.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO199_001451"
     CDS             complement(31624..31950)
                     /locus_tag="OO199_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5535543.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO199_001452"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO199_001452"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5535544.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO199_001453"
     CDS             33338..34594
                     /locus_tag="OO199_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5535545.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO199_001454"
     CDS             34709..35035
                     /locus_tag="OO199_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5535546.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO199_001455"
     CDS             complement(35176..36414)
                     /locus_tag="OO199_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5535547.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO199_001456"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO199_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5535548.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO199_001457"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO199_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO199_001458"
     tRNA            complement(40322..40397)
                     /locus_tag="OO199_001458"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="OO199_001459"
     tRNA            complement(40437..40512)
                     /locus_tag="OO199_001459"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="OO199_001460"
     CDS             40733..41092
                     /locus_tag="OO199_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5535549.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="OO199_001461"
     CDS             41094..41486
                     /locus_tag="OO199_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5535550.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO199_001462"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="OO199_001462"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5535551.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="OO199_001463"
     tRNA            43212..43287
                     /locus_tag="OO199_001463"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="OO199_001464"
     tRNA            43332..43407
                     /locus_tag="OO199_001464"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
BASE COUNT        11561 a        10820 c        10092 g        10997 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcata gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggtgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccctat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaggt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagctaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgacg
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctggtt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat accgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa
//
LOCUS       DAJTNV010000027        40860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNV010000027 DAJTNV010000000
VERSION     DAJTNV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            301..642
                     /locus_tag="OO199_001465"
     CDS             301..642
                     /locus_tag="OO199_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5535552.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(633..1559)
                     /locus_tag="OO199_001466"
     CDS             complement(633..1559)
                     /locus_tag="OO199_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535553.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1649..2647
                     /locus_tag="OO199_001467"
     CDS             1649..2647
                     /locus_tag="OO199_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5535554.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2644..2862)
                     /locus_tag="OO199_001468"
     CDS             complement(2644..2862)
                     /locus_tag="OO199_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5535555.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2864..4879)
                     /gene="ligA"
                     /locus_tag="OO199_001469"
     CDS             complement(2864..4879)
                     /gene="ligA"
                     /locus_tag="OO199_001469"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5535556.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4950..5936)
                     /gene="zipA"
                     /locus_tag="OO199_001470"
     CDS             complement(4950..5936)
                     /gene="zipA"
                     /locus_tag="OO199_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5535557.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6166..6927
                     /gene="cysZ"
                     /locus_tag="OO199_001471"
     CDS             6166..6927
                     /gene="cysZ"
                     /locus_tag="OO199_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5535558.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7112..8083
                     /gene="cysK"
                     /locus_tag="OO199_001472"
     CDS             7112..8083
                     /gene="cysK"
                     /locus_tag="OO199_001472"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5535559.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8467..8724
                     /gene="ptsH"
                     /locus_tag="OO199_001473"
     CDS             8467..8724
                     /gene="ptsH"
                     /locus_tag="OO199_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5535560.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8769..10496
                     /gene="ptsI"
                     /locus_tag="OO199_001474"
     CDS             8769..10496
                     /gene="ptsI"
                     /locus_tag="OO199_001474"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5535561.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10537..11046
                     /gene="crr"
                     /locus_tag="OO199_001475"
     CDS             10537..11046
                     /gene="crr"
                     /locus_tag="OO199_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5535562.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11088..11939)
                     /gene="pdxK"
                     /locus_tag="OO199_001476"
                     /gene_synonym="thiJ"
     CDS             complement(11088..11939)
                     /gene="pdxK"
                     /locus_tag="OO199_001476"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5535563.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12044..12418
                     /locus_tag="OO199_001477"
     CDS             12044..12418
                     /locus_tag="OO199_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5535564.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12451..13174
                     /locus_tag="OO199_001478"
                     /pseudo
     CDS             12451..13174
                     /locus_tag="OO199_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13378..14289)
                     /gene="cysM"
                     /locus_tag="OO199_001479"
     CDS             complement(13378..14289)
                     /gene="cysM"
                     /locus_tag="OO199_001479"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5535565.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14423..15520)
                     /gene="cysA"
                     /locus_tag="OO199_001480"
     CDS             complement(14423..15520)
                     /gene="cysA"
                     /locus_tag="OO199_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5535566.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15510..16385)
                     /gene="cysW"
                     /locus_tag="OO199_001481"
     CDS             complement(15510..16385)
                     /gene="cysW"
                     /locus_tag="OO199_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5535567.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16385..17218)
                     /gene="cysT"
                     /locus_tag="OO199_001482"
     CDS             complement(16385..17218)
                     /gene="cysT"
                     /locus_tag="OO199_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5535568.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17218..18234)
                     /gene="cysP"
                     /locus_tag="OO199_001483"
     CDS             complement(17218..18234)
                     /gene="cysP"
                     /locus_tag="OO199_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5535569.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18538..19329)
                     /gene="ucpA"
                     /locus_tag="OO199_001484"
     CDS             complement(18538..19329)
                     /gene="ucpA"
                     /locus_tag="OO199_001484"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5535570.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19516..20316)
                     /gene="murR"
                     /locus_tag="OO199_001485"
     CDS             complement(19516..20316)
                     /gene="murR"
                     /locus_tag="OO199_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5535571.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20480..21376
                     /gene="murQ"
                     /locus_tag="OO199_001486"
     CDS             20480..21376
                     /gene="murQ"
                     /locus_tag="OO199_001486"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5535572.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21380..22804
                     /gene="murP"
                     /locus_tag="OO199_001487"
     CDS             21380..22804
                     /gene="murP"
                     /locus_tag="OO199_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5535573.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22984..23883)
                     /gene="yfeX"
                     /locus_tag="OO199_001488"
     CDS             complement(22984..23883)
                     /gene="yfeX"
                     /locus_tag="OO199_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5535574.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23979..24554)
                     /locus_tag="OO199_001489"
     CDS             complement(23979..24554)
                     /locus_tag="OO199_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5535575.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24615..25064)
                     /locus_tag="OO199_001490"
     CDS             complement(24615..25064)
                     /locus_tag="OO199_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5535576.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25051..25476)
                     /locus_tag="OO199_001491"
     CDS             complement(25051..25476)
                     /locus_tag="OO199_001491"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5535577.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25690..26559
                     /gene="amiA"
                     /locus_tag="OO199_001492"
     CDS             25690..26559
                     /gene="amiA"
                     /locus_tag="OO199_001492"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5535578.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26563..27462
                     /gene="hemF"
                     /locus_tag="OO199_001493"
     CDS             26563..27462
                     /gene="hemF"
                     /locus_tag="OO199_001493"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5535579.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27468..28520)
                     /gene="eutR"
                     /locus_tag="OO199_001494"
                     /pseudo
     CDS             complement(27468..28520)
                     /gene="eutR"
                     /locus_tag="OO199_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28566..29066)
                     /gene="eutK"
                     /locus_tag="OO199_001495"
     CDS             complement(28566..29066)
                     /gene="eutK"
                     /locus_tag="OO199_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5535580.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29079..29738)
                     /gene="eutL"
                     /locus_tag="OO199_001496"
     CDS             complement(29079..29738)
                     /gene="eutL"
                     /locus_tag="OO199_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5535581.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30920..31711
                     /locus_tag="OO199_001497"
     CDS             30920..31711
                     /locus_tag="OO199_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535582.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32431..32826
                     /locus_tag="OO199_001498"
     CDS             32431..32826
                     /locus_tag="OO199_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5535583.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32844..33107
                     /locus_tag="OO199_001499"
     CDS             32844..33107
                     /locus_tag="OO199_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535584.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33130..33672
                     /locus_tag="OO199_001500"
     CDS             33130..33672
                     /locus_tag="OO199_001500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535585.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33811..34098
                     /locus_tag="OO199_001501"
     CDS             33811..34098
                     /locus_tag="OO199_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535586.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34137..34343
                     /locus_tag="OO199_001502"
     CDS             34137..34343
                     /locus_tag="OO199_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535587.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34655..35140
                     /locus_tag="OO199_001503"
     CDS             34655..35140
                     /locus_tag="OO199_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5535588.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35121..35399
                     /locus_tag="OO199_001504"
     CDS             35121..35399
                     /locus_tag="OO199_001504"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535589.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35392..36852
                     /locus_tag="OO199_001505"
     CDS             35392..36852
                     /locus_tag="OO199_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5535590.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36886..37695
                     /gene="murI"
                     /locus_tag="OO199_001506"
     CDS             36886..37695
                     /gene="murI"
                     /locus_tag="OO199_001506"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5535591.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37814..38508)
                     /locus_tag="OO199_001507"
     CDS             complement(join(37814..38262,38262..38508))
                     /locus_tag="OO199_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5535592.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(39027..40400)
                     /gene="eutA"
                     /locus_tag="OO199_001508"
     CDS             complement(39027..40400)
                     /gene="eutA"
                     /locus_tag="OO199_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5535593.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40375..40854)
                     /gene="eutP"
                     /locus_tag="OO199_001509"
     CDS             complement(40375..40854)
                     /gene="eutP"
                     /locus_tag="OO199_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5535594.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        10341 a        10525 c         9913 g        10081 t
ORIGIN      
        1 gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc ctaaaggcgc ttttttgcta
       61 tctgcgatac tcaaagattc gaacctgcag caggtttgag ttgagcgcag cgaaacaacg
      121 gagccgctcg cggcgacggc ccgaaaggcg agcgaagcga gtcatcctgc acgacccacc
      181 aatgcaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt
      241 tcttctcaag ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca
      301 atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca
      361 tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc
      421 actcaacaaa ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag
      481 gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaaa
      541 cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta
      601 aatcctgttg aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc
      661 cgaccagcca ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg
      721 gtggaaagtg aacggttctt cctgcgcacc agcatcaact ggcgttcaac caccggcgta
      781 atgcgcttaa ccaccagtgg gctaccttca ggcaatggta gcgccagtgc aggaagaata
      841 ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc
      901 acaatattcg cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct
      961 gacgcgtaat cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct
     1021 tccacggcca aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct
     1081 tcacattgca gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc
     1141 tggcgaatac tttccatcac ccactgctgc ggacgatcat gcaggacaaa ctgaatatct
     1201 ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg
     1261 ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac
     1321 gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct
     1381 gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc
     1441 tccttcacac tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta
     1501 aaacttttct cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc
     1561 tgttttcttc atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca
     1621 atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac
     1681 cttaaccctg atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt
     1741 ccccttcttt gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc
     1801 gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt
     1861 aatgtgcagc accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc
     1921 ggtaaatgtc gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac
     1981 cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg
     2041 ttctgcgtca gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt
     2101 gatgaatgtt cacggtgcaa ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact
     2161 gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg
     2221 caataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc
     2281 gttcagcgaa gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt
     2341 tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc
     2401 acgccgactg agcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa
     2461 gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat
     2521 gatggtgctg cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc
     2581 gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa
     2641 agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag
     2701 cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa
     2761 ccacagcaga tactcctccg gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg
     2821 cattatcgta ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac
     2881 gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag
     2941 atcctgcagc ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt
     3001 tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc
     3061 ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga
     3121 tcggcgcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt
     3181 cttcggcaaa gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt
     3241 gcagctcttc aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac
     3301 ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg
     3361 tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccaatttt ggccccatac
     3421 gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtatggacat
     3481 attctttttc aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac
     3541 ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg
     3601 tacagcgggc aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat
     3661 gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca
     3721 cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa
     3781 gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat
     3841 ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga
     3901 cgtcacgcac aaaggtcatc tgctcctgcg ccgggaattt aaacgcaacc gcccagcgcg
     3961 gggcacgcgc gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca
     4021 cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca
     4081 gcacttcttc cgccgattca caaagcgtta cccgatcgct gaccggcacc ccccactttt
     4141 taaattgcag taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa
     4201 cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca
     4261 gtgaaccagc tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat
     4321 cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac
     4381 gcgccgggat attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag
     4441 acgtgatatc ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat
     4501 tttcatacag aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt
     4561 tctcgttgtt tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat
     4621 caaaaacgtt atccagtgac agcattggta cttcatggcg tatctggctg aaagctgcca
     4681 gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg
     4741 tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg
     4801 catccatcac atgataaaga tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt
     4861 gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt
     4921 cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg
     4981 atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg
     5041 acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc
     5101 agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc
     5161 ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg
     5221 ccgctgccat ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc
     5281 gcttgttgaa tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg
     5341 acgttcatga taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc
     5401 gctacaggct caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc
     5461 ggttgcggtg ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc
     5521 tgcaacggct gttcaggctg cggctgatag gcaggctgct gcaccggctg cgctggacgc
     5581 ggagcatgtt gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc
     5641 gcagacgcat aaggcggtag gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc
     5701 tgagcgttag ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca
     5761 tcttcgacat cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc
     5821 cgatcgcgga acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa
     5881 gcgattatgg cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata
     5941 ttctctgttg ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc
     6001 cgattacctc aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt
     6061 gctttttgct gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat
     6121 acgcagaatt ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc
     6181 acatctgccc cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa
     6241 cctgggattc ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc
     6301 gcattctggt ggctctttac acagctcgat gtctggatcc cgactttcat gagttacgtt
     6361 ccggactggc tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg
     6421 ttagtgtttg gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt
     6481 ttattggctg aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt
     6541 ttcggtatca tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgtctgg
     6601 tatctgccgc gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc
     6661 gtcgcgccgg tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat
     6721 taccccttcg ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc
     6781 aaaatcacca atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt
     6841 aatctgttca tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat
     6901 cgcgataaac acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt
     6961 gaaacagggg tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg
     7021 tatatagata tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg
     7081 tatcccaatt tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct
     7141 gactatcggt cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc
     7201 gaaggtggaa tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat
     7261 ttgggatgcc gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag
     7321 cggtaatacc gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct
     7381 gaccatgcca gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa
     7441 cctggtgctg acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat
     7501 tgtcgccagc aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc
     7561 tgaaattcac gaaaaaacca ccggtccgga aatctgggaa gataccgacg gtcaggttga
     7621 tgtatttatt gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa
     7681 aggcaccaaa ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt
     7741 tatcgcccag gtgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat
     7801 tggcgctggt tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat
     7861 caccaatgaa gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc
     7921 aggtatctct tctggagcag ctgttgccgc agcgttgaaa ctacaagaag atgaaagctt
     7981 taccaacaag aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc
     8041 attgtttgcc gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa
     8101 tgcgtaaaaa agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc
     8161 ctggcattga ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg
     8221 aaattaatcg ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt
     8281 cctttagcgg cataatgctt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa
     8341 tggactgtat tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg
     8401 ctaaagtcga accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga
     8461 aatacaatgt tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct
     8521 gctgcccagt ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac
     8581 ggcaaaagcg ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt
     8641 accgttgtga ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt
     8701 aaactgatgg cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag
     8761 gtagggttat gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc
     8821 ttctgaaaga agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc
     8881 aggaagttga acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca
     8941 aaacgaaagc tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta
     9001 tgctgctcga agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca
     9061 tgacagctga cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc
     9121 tggatgatga atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc
     9181 tgcgcaacat cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc
     9241 tggttgccgc tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg
     9301 gtttcatcac cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg
     9361 aactgcctgc tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc
     9421 tgattctgga tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata
     9481 aaatgcgcgc tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag
     9541 atctgccagc tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg
     9601 ttcgtgacgt tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg
     9661 agttcctgtt catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca
     9721 aagcagtggc tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg
     9781 gcgacaaaga gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc
     9841 gcgctatccg tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc
     9901 tgcgtgcctc ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag
     9961 aagtgcgtgc actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta
    10021 aagcgtttga cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa
    10081 ttgcacgtca tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc
    10141 agtacactct ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt
    10201 caccgtccgt gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat
    10261 ggactggcat gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga
    10321 tgggtctgga cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc
    10381 gtaacacgaa cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa
    10441 cggacgagtt aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca
    10501 cgagatgcgg cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa
    10561 tctctggttt ccgacgacaa aaaggatacc ggaactattg agatcattgc tccgctctct
    10621 ggcgagatcg tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt
    10681 gatggtattg ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt
    10741 ggtaaaatct ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg
    10801 ttcgtccact tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct
    10861 gaagaaggtc agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg
    10921 gaagagaaag ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa
    10981 gaactgatca aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag
    11041 aagtaattct tgccgcagtg aaaaatggcg cccatcggcg ccatttttta tgcttccgcc
    11101 agcggcggca aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt
    11161 acgcgcaacc ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg
    11221 ataagctgag cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg
    11281 gaaatgacat tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca
    11341 ttaccggagg cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg
    11401 gcagcaatgg cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag
    11461 atattggggg taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg
    11521 tcaggtttga cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat
    11581 aggtcaggat ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat
    11641 gccgttccca tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga
    11701 agcgcacgca aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca
    11761 taatgcggcg tattgctcag caataccgtc ggcacggcaa agacattcaa gccgttctgt
    11821 tttatagcag gcaccgcaat gctgttgcct acgcttccgt acactacctg cgactgcacg
    11881 gcgacgatat ccgcctgcag cgccctactc ttatcgttaa acaacaacaa actactcatt
    11941 taattttttc tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct
    12001 ggcagtcaaa tcggctatta caaaacaaag ataaggtaat tcagtgaaga aaatcatttg
    12061 tctggccatt acgctgctaa tgacactccc cgcttacgcg aagttaactg ttcatgaaga
    12121 agcccgcatc aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg
    12181 caacggtgat gaacatacct gcgatgaagc ggtttcccac ctgcgtctga agctcggcaa
    12241 tacccgtaac cgcattgaca ctgccgagca gtttattgat aaggtagctt cgtcgtcatc
    12301 gattaccggg aagccgtata tcgtgaagat ccccggtaag agcgatgaga acgcacagcc
    12361 ttttttacat gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc
    12421 cgctttggta aggcatttac aggaggtaac atgaaaaaat gctttattta tcacgatgaa
    12481 aaatcgaata aattttggtg gatagattac gaaggggata gtttagctgt caactatggc
    12541 aaggtaggta gtattggtaa attccataca aaagaattcg ataatgaaga acagtgtctg
    12601 aaagaagcca gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga aactccagac
    12661 tttaacttca tggatctcta ctattttgat gatgaagaga ttgggttaca tcttaaaacg
    12721 tcacacccaa acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa
    12781 gaatctcctt ttggtagcga tgaaggtgct gatgctctaa acgaacttga aaatagcctc
    12841 cgttctggac tgtgctgatt tccctcaaat gttaattgaa actatgtggg gtatgaaata
    12901 catcgctatg gacagtattc ttgaagagga tgtgcgtgcg caattactag ccgatgaaat
    12961 gagctctatc cagagcaata tgattaccta cgcgactgca ttcggtcaga tcaaagtcat
    13021 gggtaaaatc tcccataaac ttaaaaagat gggactcaat gcactagcgc gtcatcagct
    13081 taccgcaaaa attcttcaat ggggtgacgg tcaggactca ccaatacttc aaaaaatgat
    13141 tgatgacctt actgcttttc cacacgaaaa ttaaaaactg catttgtcgg cagcaacaac
    13201 tgccgacaaa tcagattatt gacaaagtgc actttgttta tgccggatgc ggcgtaaacg
    13261 cctgatccgg cataaaaata ttgctaattc aatatattgc agaaacattg taggcctgat
    13321 aagcggagcg catcaggcag ttttgcgttt gtcatcagtc tccaatgcta ttaatcctta
    13381 aatccctgcc ccctgactaa aatgctcttc cccaaacact ccggtggaga ggtagcgatc
    13441 gccacgatcg cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag
    13501 tgctccagca accgcgccgc ctgagctgac gccacagaat attccttccc gcaccgccag
    13561 ttcgcgcatg gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagcgc
    13621 agcgttgaaa atccccggca gatattccgc aggccagcgg cgaatgcccg gaatactgct
    13681 gccttcttcc ggttgcaggc caacaatggt caccggtttg gattgttcgc gcataaagca
    13741 tgaaacgccg gtgatagtgc cggtcgtccc catgctggag acaaaatgag tgatgcgccc
    13801 gccggtttgc tgccagattt ccggcccggt ggtggtgtaa tgcgcataag ggttatcggg
    13861 attattgaac tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc
    13921 gcgcgcacct tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg
    13981 catcgccgca cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc
    14041 tttcagcgcg gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa
    14101 gacatcaccc ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc
    14161 acgatctttc accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc
    14221 gttatccggc cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa
    14281 tgtactcaca atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc
    14341 aggcctacag gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg
    14401 cgtttaacat tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatcgcggg
    14461 tttcaatacg ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct
    14521 cgccatgctg cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc
    14581 ccagcggctg caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta
    14641 ccggcagcgg cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga
    14701 agagatccac cggcccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa
    14761 catggaactg cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga
    14821 gtacaaaacg ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat
    14881 tcccctgact catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg
    14941 tcacaaacac gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct
    15001 ctttacgcac ctgcgcatcc agcgcgccga acggttcatc aagcagcaga atttgcggtt
    15061 ctacagccag cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa agctgcgccg
    15121 gataacgatc cgccagatgc gcaagctgga ccatttccag caattttgtc actttcgctt
    15181 tgatggctgc ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagtgatat
    15241 tgtcgaatac cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac
    15301 gatcgcgcgc gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt
    15361 gatgctccag cccggcgata atccgcagca gcgtggtttt cccggaaccg gacggcccca
    15421 gcaacgcgac catctgacca gaaggaatat ccagtgagat atcgttcagc acctgggtgc
    15481 gaccaaaaga cttcttgata ttggcaatct caatgctcat gatgctcctc ctgctgcgcg
    15541 cgtttttcct gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc
    15601 gccatcaacg tcaacagcgc ggcggcggta aaggagccga cggtgttgta gtcctgctcc
    15661 aacaattcaa tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc ggaaaccacc
    15721 gacactgcac caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc gtaaagcagc
    15781 gcccagcgaa tattcggcaa tgtgacgcga cggaacatct gccagccgga cgcgccaagc
    15841 aaaatcgccg cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc
    15901 accacaaacg gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata
    15961 atttgcagat tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc
    16021 agatacacca gaccggcaac caccggcgat acagcaaacg gaatgtccag tagcgtcagc
    16081 agtaactggc gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac
    16141 accaggttta ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc
    16201 atgtccggat cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg
    16261 aagatgtaaa tcatcggcac cagcaggatg aacgccgaaa ccagcatccc gatgccaatc
    16321 agaaaccatt tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc
    16381 gccattaatg acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca
    16441 gcagcagcag agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc gggtaatcaa
    16501 actcctgtaa gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac gcgatatttc
    16561 cggcgataaa aatcaccgcg ccaaattcac caagactacg ggtaaacgac agcgccacgc
    16621 ccgccaccag cgccggagaa agctccggca gcaccacttt gcagaaactc tgccagcgcg
    16681 ttgcaccaag cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct
    16741 gcacggtacg caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc
    16801 aggtataggt gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg
    16861 aaaagagcga ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca
    16921 tcagcgcatc aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca
    16981 gcagaccgaa aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt
    17041 aggccgcgac cacctgcggg ttggtgatca cctcccagta ctgcgcccag ctcatctcag
    17101 aaagctgcat caccagcgca gagagcggca gcagcaatat caggcacaca aacagcagac
    17161 tggtgccaag gcttaaagta aagcccggca gcacgcgtct tgaagaaact gcaaacatta
    17221 cttacgcccc gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat
    17281 cacttccggc catgagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt
    17341 atctttcagt ttgtccatga cttccgggtt attcacacgg tagtaatagt cggtgatgat
    17401 ggtctgcgcc tgtgggctgt agagccagtt caggtaggct tttgcggctt tttccgtacc
    17461 gttggcctgc acatttttat cgacccacgc caccgggaat tccgccagaa tattggtttt
    17521 cgggatcacc acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga
    17581 ttcaaagcta atcagcacat cgcccaagcc acgctcggca aaagtggtgg ttgcgccacg
    17641 accgccggta tcgaacactt caacgttttt caggaactgg gtcataaact gttcggtttt
    17701 ggctttatcg ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg
    17761 cgcgttaccc gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc
    17821 gttccagtcg tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta
    17881 gaacggcgag ctattattcg gcaggcgcga ctgccagtca gccgggatca gcttgccttt
    17941 gtcgtgcagg atttgtacgt cggtcacctg gttataagtg acaacgtctg cttttaagcc
    18001 ctgcaaaatc gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt
    18061 gtcgccgccg ttatcttttg cccattgctg ttcaaacggc ggattcaggg cggcaaacag
    18121 ctcgcgggag acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag
    18181 cagcagagaa gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca
    18241 cccttataaa tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt
    18301 aacggtttta tataccgttt ggtgatttgg aagttaaaaa gggaataaga acttggttca
    18361 acaggcgtaa ttgttgcagg cagtttggac acggacagcg cggagaaacc ggagcgtaca
    18421 catagtacgt gaggatttcg agcactgccc ggggccaaaa tgacaaatga aatagcctgg
    18481 tgaacttagt tcaagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca
    18541 gataccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
    18601 ggttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
    18661 atcggcgagg cgacacagcg ggattgcttt tgccatttca gtcagcaccg attctggatc
    18721 ttccgggttc gactggcggg caatgctttc cgccattggc gtgcggacgt atcccgggca
    18781 gatggcgtta acgcgaatac cggattgcgc gtactccacc gccagcgatt tagtcaggcc
    18841 aacaatcgcc gctttcgtta aggcatacgc cgtttcgcca ggatcggcca ccatatcacc
    18901 agtgactgaa gacatcatca caatgcgacc atctttgcgc gcaatcatct ccggcagcac
    18961 cgccttcgtg acgttccata cgcctttaat attgatatca atatggaaat cgcgatcttc
    19021 atcgctcata tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc
    19081 aatgcgccct tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg
    19141 cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt ccgctagctt
    19201 ttcgatctca ggggagatat ccagcaagat taggttcgcg ccatgacgtg caaaagttct
    19261 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
    19321 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
    19381 atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc
    19441 agtcgctgct ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt
    19501 ttcaatgacg ccacatcatt gatgttgcac aagaccaaca aacagtaaat cggtaacaga
    19561 attttgcgcc gtgcgggtag acatcgacga gctacgccat tcggtttcac cggaaacggt
    19621 atcgagcgtg aaatgcgcca gccgccgcag cggagagtca gccagagaag taatggcaat
    19681 caccgttgcc ccctgctttc gtgccgcttc tgcgcacaag acaatctctt ttttactgcc
    19741 gctgtaagag atagcgattt gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg
    19801 tacgtgcgtg tcggcttcgc aggccacgcg atagccaatt ttcatcagtt tgaacgacag
    19861 atcgcgcccc accagcgccg atccgcccag gcccgttatc tggataaacg gtgctttact
    19921 gatgacctca atgatttttt gcagccgcgc gtagtcgaac aacgcacagg tttgctccag
    19981 cgccagctct ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat catcactggt
    20041 aatcgaactg tgcaggtgca gcgccgtggc attcgttttc tcccggctgg cactgtattc
    20101 gccaattaac gccatacgca attcagtaaa tccttgcgcg ccgagcttct gggcaaattt
    20161 cacaatgctc gactggctga taccgagctg tttcgccatc tgccgtgaag agacggattt
    20221 cagctcgctc actctggccc gcagaaagtc agcaattttt tgctcgttcc cggtaaattc
    20281 actttccgca ttgcgtattt tcgtcaggta caacatcttc cactctcctg ggctggcagc
    20341 aaaaattccg cttaagttct tatgacgctc ttcacactct gcgagtgtag actcaatgat
    20401 tcctttaagt ctgtcaattc gggatatatg attccataca tctgcaatta tttgatcgta
    20461 aatagtaagg tcaccaccga tgcaactaga aaagatgatt actgaaggct cgaacaccgc
    20521 ctcggctgaa attgaccgcg tatcgacgct ggaaatgtgc cggattatca acgatgaaga
    20581 taaaaccgta ccgcctgccg ttgatcgcgt cctgccggat atcgccgcgg cgatcgatgt
    20641 tatccacgcc caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg
    20701 tcgtctgggg attctggatg ccagcgaatg tccgcccacc tacggcgtga aaccgggtct
    20761 ggtggttggt ttgattgctg gaggcgaata tgccattcag cacgcggtgg aaggagctga
    20821 agatagccgg gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg cacaggatgt
    20881 ggtggttggc attgctgcca gcggtcgcac gccgtatgtg attgccggac tggaatacgc
    20941 acgccagctc ggctgccgca cagtgggaat ttcctgtaat ccggggagcg ccgtttcaac
    21001 caccgctgag tttgccatta caccgattgt aggtgccgaa gttgttaccg gttcttcgcg
    21061 gatgaaagca ggtacagcgc agaaactggt gctcaatatg ctttccaccg ggctgatgat
    21121 taaatccggc aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact
    21181 gcatgtgcgc caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga
    21241 agcggcgtta attgcctgcg agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa
    21301 tctcgatgcc gcagaagcta aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt
    21361 tttagacaag gaataaccca tggccaaaga gatcagcagt gaacttctga acaccattct
    21421 tacccgtgtc ggcggaccgg gaaatatcgc cagttgtggt aactgtatga cgcgcctgcg
    21481 tctgggtgta catgacagtt cactggttga tcccaatatc aaaacgctgg aaggcgtgaa
    21541 gggcgtcatt ttgaccagcg accaggtgca ggtcgttttt gggcctggta aagcgcatcg
    21601 tgccgcaaaa gcgatgagcg agctgctggg cgatgcaccg gtacaggatg ccgcagaaat
    21661 cgccgcccag aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc aacaatttct
    21721 cgccaaattc gccaccatct tcacgccgct gatccccggt tttattgccg ccggtctgct
    21781 gctggggata gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg ctcagggcac
    21841 gctacccgac gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca ccttcctagt
    21901 gattctggtg ggctataacg ccgcccaggc atttggcggc acgggcgtca atggcgcaat
    21961 tattgccgca ctctttttgc tcggttataa ccccgctgca accaccggtt actacgccgg
    22021 ttttcacgat ttctttggtc tgcccatcga tccgcgcggc aatattatcg gcgtgctgat
    22081 tgccgcgtgg gcctgcgccc gcattgaagg catggtgcgc cgctttatgc cggacgatct
    22141 cgacatgctg ctgacctcgt taatcaccct gctgatcacc gctacgcttg cgtacctgat
    22201 cattatgccg ctgggtggct ggttattcga aggtatgtca tggctgttta tgcacctgaa
    22261 cagtaatccg ttcggttgtg cggttttagc cgggctgttc ctgatcgccg tggtatttgg
    22321 cgtgcatcag ggctttattc ctgtttacct cgcgttaatg gacagccagg gattcaacag
    22381 cttatttccc atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta
    22441 ctggcgggcg caaccgcaca gtgcgctacg cagtcaggta cgcggggcga ttattcccgg
    22501 cctgctgggc gttggtgaac cgctgattta cggtgtcacc ctgccccgca tgaagccgtt
    22561 tgttaccgcc tgtttaggcg gcgcggcggg cggtttgttt atcggattga tagcctggtg
    22621 gggtctgccg atgggcttaa acagcgcctt tgggccgtct ggtctggtgg cgctgccgct
    22681 gatgacttcc gcacaaggca tccttccggc aatggcggtt tatgctggcg ggattctggt
    22741 ggcatgggtt tgcgggttta ttttcaccac gctctttggc tgccgtaacg tcaatctgga
    22801 ctgatattat gaaacggaca atgctctatc tttctctgct ggctgttagt tgtagcgtca
    22861 gcgccgcaaa ataccctgtt ctgacagaag gtagttgatc aagtatatgc ggcgttaaag
    22921 cagtaataaa aaggccagtc agtaatgtta ctgcctggcc tttaatcaat gaatcagaaa
    22981 cgcttacagc gccatcaact tgtccagcga cggtgcgaaa taatagccgc cggttaccgg
    23041 tttggtgaaa cgcaacatcg catcacgttt gccatcggta tcgccaaaca tgctcagcag
    23101 ttgctgctca atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc
    23161 actggcggtg ccgtacggca ggctctggcg aacaatcttc agccctttgc catcttcttt
    23221 cagatcaacg cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt
    23281 ggcctctttg gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc ggttgagctg
    23341 cttcaggttg tgctcccaac gctgcacaaa cacatagctg ccgccggaat ccacgccgtc
    23401 tttgataacc gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac
    23461 aaagccgctc aggtcacgct cttccaccca acggaagccg tggatctctt ctttcacttc
    23521 aatgcagtca ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta cgtcgtgacg
    23581 cagagaaaga atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc ctttaccgta
    23641 gcccggaaaa tctttcagct cttctgcccc aacgccgccg ctcagagcgc gccaggtgtt
    23701 gttaccaaag gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc
    23761 cagtttgtcg gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt
    23821 ggcttcgatc caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga
    23881 cattgttcct cctgaaaata agaatgccac gcaatccgtg gcattgttga gcgctatttt
    23941 acgtgttttt tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca gataatttta
    24001 ctgactttcc agtttttcag ggtatcgtcc gaaggcatta acccttcagg accacgccat
    24061 tccccgctga actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga
    24121 ttatcatcac catcagcttt ttggcaattg ccaaatgctt tgctgtaaag gtcgctaaac
    24181 ggtgtaccga ttttaacacc ggtgtcagca ggaatatcgc tatccagcac gtcaatgcga
    24241 ctgatcgtgc cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt catcacttca
    24301 aaaaagcgca ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg ataatcgcca
    24361 tcaagcgcat cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg
    24421 ccctgctcgc tcactttggt ggacgatcca aaccagttcc acgggttagc ggcagaccag
    24481 ttaactgacg acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa
    24541 cgcagcgatt tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc
    24601 accaaaaagt gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc
    24661 agcagccaga ttaacgccac aatatccgcc actaccagcg ccagaccgat accgctaacg
    24721 gattcaccgt tcagccacag ccacggttgc cagcaaagta aaaccacctg cgccaacagc
    24781 aacagaaaat agagcacatg ccaggtgcga ggaaacgaag cccgccgacc gctcaacaaa
    24841 aacgccagca ccgccggaat accgggaatc agccccagcc agaaattatc gtgatcggga
    24901 taaaacagat ttagcagcgc agtaccctgc tcacgcgacg caccggcaat gacaaacagc
    24961 acccaggttc gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcgt
    25021 ccgtgcgcat cataatggac aggatggaac tcagtactct tcatcttcaa tcaaacgctt
    25081 acccagactc agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat
    25141 gtcgttatct tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa tcagcttttt
    25201 ctccagccgg ttaagcaacg cattggcaat tccacgccca cgaaactctg gatgcacgcc
    25261 aagataataa gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc
    25321 accgtttacc tcagcgacca aaaacaaact gacgtcatgg ttcatcttac gctcgatgtc
    25381 catttccgga tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg tgatgacctc
    25441 ttcgaaatct tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt
    25501 taaaaggctg attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga
    25561 aaaagtggca taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca
    25621 gcaaaccgtc gtaacggatt acgcgatacg atataacatc tggaacttta ttattacaac
    25681 tcaggccgta tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg
    25741 aaagccggtt tagctgccct gacgttgtca ggaatgtcgc aagccattgc caaagacgaa
    25801 cctttaaaaa ccagcaacgg acacagcaag ccgaaagcca aaaaatctgg cggcaaacgt
    25861 gtcgttgttc tcgatccagg ccacggcgga attgataccg gagcgatcgg acgcaacggt
    25921 tcgaaagaaa aacatgtggt gctggcgatt gctaaaaacg tccgttccat tttgcgtaat
    25981 catgggattg atgcgcgttt aacgcgttct ggcgatacgt ttatcccact ttacgatcgc
    26041 gttgaaatcg cccataaaca tggcgcagat ctgtttatgt caattcatgc cgatggcttt
    26101 accaacccga aagctgccgg tgcttcggta tttgccctct ctaaccgtgg ggcaagtagc
    26161 gcaatggcga aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaag
    26221 gcgactgaca aggatcacct attgcagcaa gtactgtttg atctggtgca aaccgatact
    26281 attaaaaaca gcctgacgct tggctcgcat attctgaaaa agattaagcc ggtgcataaa
    26341 ctgcacagcc gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttcct
    26401 tcggtgctgg tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg
    26461 gcggcgtttc gtcagaaaat cgccacagcg attgctgaag gcgtgatcag ttatttccac
    26521 tggttcgaca accagaaagc acattcgaaa aagcgataag ttatgaaacc cgacgcacac
    26581 caggttaaac agtttctgct caaccttcag gatacgattt gtcagcagct gaccgccgtc
    26641 gatggcgcag aatttgtcga agatagttgg cagcgcgaag ctggcggcgg cgggcgcagt
    26701 cgggtgttgc gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc tcacgtccac
    26761 ggtgaggcga tgcctgcttc cgccaccgct catcgcccgg aacttgccgg gcgcagtttc
    26821 gaggcgatgg gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc caccagccac
    26881 gcgaatgtgc gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg gtggtttggc
    26941 ggcggcttcg atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc
    27001 accgcccgtg acctgtgcca gccatttggt gaagacgttt atccccgtta caaaaagtgg
    27061 tgcgacgatt acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc
    27121 tttgatgatc tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc
    27181 aaaggctaca ccgacgctta tttaccaatt gtagagcgac gtaaagcgat ggcctacggc
    27241 gagcgcgagc gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc
    27301 tgggatcgcg gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg
    27361 tcaatgccgc cactggtacg ctgggagtat gattatcagc caaaagatgg cagcccagaa
    27421 gcggcgttaa gtgagtttat taaggtcagg gattgggtgt aactccctca cccccattcc
    27481 cgcatccgct gatgcaacgt caacgactgc ttctcggcaa acagctgctg gtaatccgtg
    27541 gcaaattgcc ccagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt
    27601 tgcgaccacg ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg tttcagccac
    27661 gcgtttgggc caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg gcgactgaca
    27721 tgcagttgat tacacaaatc cagcaccgtc accggttcgg acatgttttc cagcacatat
    27781 tcacgggcgc gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc
    27841 attggttgcg cttcttccag catcgccccc atcgccatta gcaaattatc ccccagcact
    27901 tttcgcactg caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt
    27961 tggacaaagc cccacagcgc ggctttatgc tgctctttca cttccagcgc cgactggcta
    28021 cgcagcatat gtaatacccg atccggattg tgcaaaaagt tagcctgaca ggtgatgaca
    28081 tcttcagaca gcactacgcc caggatcgta taatcgtccg gcgtacttaa ttcaaactcg
    28141 gttccaccgg ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc gataaatccc
    28201 tgctcaccgc gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac
    28261 tgacgcagcg ccagaccggt gtattcacga aaaacctgaa tatcatcgag tagaatttcc
    28321 gtaaattcac catgaaactt gcccggatgc agctgatcgt aaatctgctg ctaggcggta
    28381 atcgtcaaag catgttcata gacatccgtt gtctgtcgtt gatgaacatt atccacttcg
    28441 accttcggcg tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg
    28501 gctgtacggg tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg
    28561 acaggttaat ggggcttgag acgataacga ctactgcgtt tacgtaacgt cccggcagaa
    28621 aagagctgtt cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcggccact
    28681 tctcctgccg tcattccctg acatactgat gttaacagcg ccaacaactc gtcagcagat
    28741 tccgcgacga taactggcgc gtcgggttcc tttacgggtt gcttcggctg gcggttaaag
    28801 ccagtaacca gccactgggt gtcatcgtct ggccgaccga tctccttacg gctgatgaca
    28861 cgcccggtac gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga
    28921 tcgccttcca ccaccagcgt taagcgacca gggtcaagca cttcgtgact gagcagacga
    28981 acgttagctg ctttgagcat ggcatccgct gcatctatcg cggcgaccat gccgtccact
    29041 tccagcaatc ccagtgcatt gatcatcggc aacctccgtt acgcacgctg gattggatta
    29101 cgcgcgattt ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg
    29161 ctaccggtta aaaatgctgc cgagtagttg gtttcagacg gtggcgggac gtaggtcacc
    29221 agctgtacgt cggcagattt caacgcggca tcaatgccgt aggtcgcttc cagcggcggt
    29281 gccaccagat acgccatcgg atcgccaagc gtgatcccgg cggttgatga aagataagaa
    29341 ccggtacgcg aaaccacatg cgccaggaag gcggtgtttt ctgcgtcatt ggcccactgg
    29401 aaagctgcgc cattttcaat atgtgcaacc atcgcatcca gaccggcacg cacttccgcc
    29461 gggtttggcc caccaagcat aatcagcact tcaccggcag tcggtgacgg gccgtgtgcc
    29521 gctcccgcat acagcgagcg accatatacc acttcgacca tcgcttgttt ggtcgcttcg
    29581 tcagccgcaa tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag gctacgaata
    29641 tgcggcggca attttagttc acgcgcaaat tcagcgttaa cggaggcaat cacccgcatg
    29701 gcggttaccg acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg
    29761 ttgatgccgg atgctttctg ctccagcata cgtttcgcca aatccacaat gacggcacag
    29821 cagcacgaaa gagaagcaaa agtcagctac ctggtacact aggcagcggg tcagcaaagg
    29881 ggggcaaatg cccctttttt catgtcagat aatcagatca ttacctcaat aaactaataa
    29941 tcacatcgcg gttacaacca gtttgatagt gatagctcaa acccagacgt ggcgctggct
    30001 gcaagcaaaa taagccgcaa aaatgcgacc ccatgtttac tagccgcaaa aatgcggtcc
    30061 tatttgccag aaccttttat tattcaattg gttagtgcaa gaaaaagcgt gattactgtt
    30121 caagaagtat ccagaaaggt catgattttg acgctgaaaa aaaataggtg ggcatttttg
    30181 cccactcact agccgcaaat ttgcccactc actagccgca tttttgcggc ccaaagtaat
    30241 aaatacagag aaataataaa tacagatcag cgccaagatt tttcgtcttt tcggcggaat
    30301 catgagacta gtgcttacgt cctgataacc tccagagcag accttaactt ctgtgcagca
    30361 ggactacgct cattgccgct cggctaggtc aaatcctcac gataaagctg ttcggattgt
    30421 gaccataatg ttaaaggact ctttaaatca ttccttgacg aattaactag ctggcttaag
    30481 cggaatagca tgcaggataa aaacaaaacc agcttagcag tggggagagc ttcgcttcgc
    30541 tcgtctcctg gggccaatgc tcaatgaaac gacgtaaggc acggagcgct caccataggt
    30601 agacacataa acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat aaaaaccaac
    30661 gacaccgcca cactgcaaga ccaatggttt ttatcgcgta tacagaccag acaggaaaca
    30721 gcagcaaagc agcgtatagc caggtgcaga cagcacactc acacgacgca cagcaggctt
    30781 aagtgatcac tcaggaaact ttatccccgc cgccgatcac cgcgaactat catgaccgcg
    30841 ccaaattcga aaggaatggc ccatagaacg cgatgaacac acataagcgc tacacttact
    30901 taacgtcgcc acagagcgat tgagagcgct taaaatcgat gcgatttcat taaatttttc
    30961 tcactcgcct gaatggtggc gatcaggact gatcgcaaca ttgccccgcc tcgatattga
    31021 gcgcataata gatacataca ttaagcggaa aagagataag tttattgtga aaatcagggg
    31081 gttacatatc atgcgcatat cagaacaaca acggcagatc ctcgactatc tggcaaaaaa
    31141 cgaatcaatc accgcacgac aagccgcaga tctcgtttat ggtggcaatg ttacccgcgt
    31201 ccaggttgac gccgcccgcc gctcactcat gacgatggtt agtaatgggc taatcaggaa
    31261 acaaggccgc gctttcgtag ctgaaagaca tatcaatcac gcttatagcc aggacgttga
    31321 agaattgcgc cgccagatcc tttgggtatt cagcaaacag ggtcaggagt atttcctttc
    31381 tgaacaccca aatttcgccg ccaaagagtt aacagtaagc ggattgtcgt gcgcactgga
    31441 ttatcttgtc ggtgaaggtt gcgttactta caagcagcta tggcacgccc tgaatacgct
    31501 tgtagaagat ggtttactca ttgccacgcg tgagaacatc gaatactgga taatgccgcg
    31561 ttcaggtcgc cgcaatcatg cacggtatat ttcaggcaac agatacagaa cgccggactt
    31621 taatccgaca caggaagatc gggaccgtgc cgaaatgatg tgcagggaac aggagaaaag
    31681 aattatggat aagttatttt cgatgcagtg aacacaagcg ggcgcaatgc ccgtttttat
    31741 tgccttattc cacgcctgac gaatcaattt gatcacacct gagaaaatgc atagccagaa
    31801 gttaaggccg ttatattgcg attgggagcg ttttaaacgg tatcaattta attaattctg
    31861 atgtggtgat ttgcaggcaa aaaaaacccg ccggagaagc gggaaataga gacatatcgt
    31921 tgtagttatt aacagatcac tttgaggtcg agggcggcta accctcgcta atattatccc
    31981 gctattacct tgcgggtgca aataatcttt tattgctttg atgatttaat aaatagcact
    32041 ttttgctaac aaaaactgct gtcaatatat ggccaaaaat gtcaacagaa aaaataaggt
    32101 ttataatatt aagtatcaca tgggtaatgg gttcagggaa tcggaatcca ccgcctcacg
    32161 gaaattcaaa atttgcttct tctttcctcc gtgggcggct tctttttcgt atcgaggtat
    32221 ttatgaaagc aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc
    32281 tgaggcgctg gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta
    32341 ctcgccaatt attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat
    32401 aatcacgcat cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt
    32461 gccgatatta tgcggcgcag atggatttat gaccctgaag cgggtttatt gtttagccgt
    32521 gaaacaaaag acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta
    32581 catgaaggta aattcaaagt gacactcacc tatcagaaag cctgctatgt atacacatac
    32641 ggcgcttatg acgagacatt atatgaagtg caccacgtta atttgaacaa acaggataac
    32701 cgcatcaaga atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc
    32761 cgcaaactta ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca
    32821 ctataaacac aggagtaaat accatgcatt cattagacag ttattttcag cgcacgacag
    32881 cccctaaatc agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg
    32941 ctgattatat tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg
    33001 ataaacttca gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt
    33061 gcgaattaag tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg
    33121 ggaaagataa tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag
    33181 cgtgataaaa ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc
    33241 cgtatggccc agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc
    33301 ccacaaatcg ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta
    33361 aaaacgaacg cagagaaaat ggagatgcac aaacagagcg gtgctccgaa tcccgatgag
    33421 tacttctcac cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc
    33481 accgaggaac agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac
    33541 tcgtatttcg gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa
    33601 ccagaaatgg ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg
    33661 attcggtact gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga
    33721 ccaatgaaca aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac
    33781 tatattagca caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc
    33841 gctaagaccc tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc
    33901 atgaagcgcc aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc
    33961 atcgacatga aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt
    34021 ttaattctca ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat
    34081 gatgggacgg gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga
    34141 agcaagatat tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac
    34201 tacgtaagca ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca
    34261 tcagtttcac caattactgc atctacgagt gttacaagga agatgcagag gactggctgg
    34321 attcattgcc agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc
    34381 atatcacctc tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc
    34441 acaataacga tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat
    34501 tgaaacacat ctaccatgac cataataaag taattttttg atatgtttat ggttttatat
    34561 taattaaaat atatctaaaa ttgatagttt taaaattgca aaatccagat tgtgtttgta
    34621 atgtttaatc tgattgtatt gagtggcgtt tattatgaaa aataaatcag gtaattcttg
    34681 ttatagagaa gcagtcgata ttcatagctc acttgagctt tcatttatat tgcataaata
    34741 tcatttgcgt gtagatgaga agaaatctcg aaatgtattc agcgccatac gtgatgagct
    34801 catccttgat ggggattcac gacaaaactt agctactttc tgtcaaacat ggcttgatga
    34861 tgaaatccgt gagttgctta atttagctat tgataaaaac atgatttata aagatgaata
    34921 tccacaaatg gcggaacctg aaggtcgctg tgtgcgtatg ctttccgatt cgtggagttt
    34981 ccccgattcg cgacacacct taggttgctc aaccattggt tcatctgaag ctgctatgct
    35041 cggccgcttg gcgttaaaat ggcagtggtg taaaaaacgc gaagtacaag gtaaatcaac
    35101 cgaacctgat ttgcgatcct gtgcaaatat gctggcataa attctgccgt tactgtgatg
    35161 tgaaattgtg tgaaattcca cttgatgatc atcacctgat aatgaatgct aaagaggttt
    35221 tgaagcatgt cgatgaaaac actattggtg tcgttacgac tttaggtatt acttattttt
    35281 gtaaagcaga ttgcgttagt gtttttaaga taagtgttaa agttaatatt agtttttata
    35341 tttacatcgt aattatatgt ttctatttta aaaatgttcg agagtataaa aatgtctaat
    35401 gctaatatcg cacccaaaag taagaaaatg acagtgggga cactcgctat catgaatata
    35461 actgctgtag taagtctacg atttatacct tctgaagcag aatacggctt aggtgccatt
    35521 ttttactata cctttgctgc aattatgttt ttagtacctg ttgcctttgt tgcggctgag
    35581 ctagctacaa catatccaca aaaagggggg gcatttcgat ggataggaga agcatttggg
    35641 gctcgctggg gttttgtagg aatgcttatg acctggctac aagtcatccc ctactttcca
    35701 acagtactga catttggtgc agtctcggtt gcatttattg atgttgatat gggggtggca
    35761 gaatcaattg ccagtaataa atggtatatt ttatttttcg tactatttgt ttactgggga
    35821 gctatttgtg ttgcattacg tggcgttggt attttctcgc aggtatctaa gtggtgcggt
    35881 attataggga caattattcc agctattatc cttgttattc ttggtttctc ctatctgtgg
    35941 ttcagcggta aaccagcggt gatcccatta ggttggggtg atcttttccc taatatagct
    36001 aacttccaga atattgttct tgccgccagc atatttcttg cttatgcagg gatggaaatg
    36061 aatgcggtcc atgttaacga tttagataat ccaactaaaa aatacccaat tgcaattacc
    36121 atcgcttcta taggtactat tgcaatattc ctgctcagta ctttaggggt cgcctttatt
    36181 attcctacag ataaaattag tctaactcaa agtttgttgg ttgcctacga tatgctgttc
    36241 gaatgggctg gagtgccatg gcttggctct gtgatggcat ttatgctagc aattggcgta
    36301 ctgggtgggg tggttacttg gattgcaggg cctaatacag gtgtattagc gatcgcgaaa
    36361 gctggttatt tacctaagtt tttccaaaaa accaatcgtc atggaatggg gcaccatcta
    36421 atgtttgtac aaggtataat tgtctcagtt ctttctgtta cttttgtgat aatgccttca
    36481 gttcaagcag cttttcagat tttatcacag ttaacagtta tgctctattt agttatgtat
    36541 atgctcatgt tcgctagtgc tatctatcta cgacattctc aacctgatac aaaacgccca
    36601 tatcgagccc ctgctttatt tttctgggca acacttggat tcttagggtc ttttctggct
    36661 tttgtattaa gttttattcc gcccgagcaa atcccaactg gtagtccaac cagctatgtt
    36721 ctttatttgt tagtattagt tgccctattt ttattaattc caatattaat ttacgccttc
    36781 cgaaaatctg actggaaaga tccaaactct gatttcgaac cattttcttg ggaaaaaaga
    36841 actagtaatt aaattttcca taagctacaa aagatgaggt taaatatgtt tagtgctgaa
    36901 tctccaattg gtgtaataga ctcaggtgta ggtggattga catcggtaaa ggaaataatt
    36961 aatttgcttc ctggtgaaga tattatctat tgtggagata atatgaatgc accttatggc
    37021 aaccgttctg ccgatgatat catcagtttg actaaaaaaa tgctgacttt tttacagtca
    37081 aggaatgtca aactagttgc tgtcgcatgt aatactatat cctcaacttt ggaatccgaa
    37141 gaatacagcg gatatgctaa gagctttccg tttcctatct tatccatcat tgaacctgca
    37201 gtagaagatg tgattcgcca gcaatataaa aatgttggta ttatcgctac tgagtttact
    37261 attaaaacgg ggtgtcacaa agagctaata aaaaaattga attcaacaat aaatgtattt
    37321 ggagaaccaa gcaaaaatct tgcaatgtta attgaggaag gtaatcttaa tgctcctgca
    37381 atcttgaatg acatcaaaaa acatgttaac catttaattt ccctccatcc agtaaatgaa
    37441 attattctcg gctgtactca ctatccaatt gttcagaatt tttttgaagc agttgcaccg
    37501 gatataaaat ttattaatcc tgcacatgat caggctatct ctattaaaaa tcacttggga
    37561 cagctaaatt tattaaactc ctcaaatata ggaactttgc aaataaatac tagtggaagt
    37621 atggagatct acaaaacggt actaggggaa ttgagcatta cgaaacctca tacattcagt
    37681 atcaggcagt tttaaattta tccggtgcac cgtaaaatcc cgtccttctg aactgcaccc
    37741 caaaagttgg acaccaactg agtaaggtgc attttatgga caaacccagt tacatactgg
    37801 gtagtgcatc caattagtag aacatgtgtt tttcgataaa cgctccgatc actttttcgt
    37861 ggatctcaac tgagcgtgag aagcagattg ttttacgacc caaccgctta atgcgggtgc
    37921 gtagcgtcag attattacgc tcaatgcgtt gggtgaatat tttgccggtc agatgcttat
    37981 tcttcggcac ctcccggcca tagctgcccc agtcatcgct ggtcagcatg ccgatgttga
    38041 agggtgtaag cagtgccagt agctcccggc acgtttgatc ggttcgggga ccaaaagtgt
    38101 aggccagtac accgcctgtt ttggtgttgt acgcgtaccc gagccaatgt tgccgggctt
    38161 tactgccaac gtagctccat tgctcatcaa gctcgcagat aaacgccaca tcagcatggg
    38221 caacaggcga agaagttatt cgctttggcg cgagtttttt aaagtccgga tgacggtgtt
    38281 actaccaatt ttcagtgtcc tggcggtatc gcgaaccccg gcaccattaa aggccatttc
    38341 agttatcagc tctttcatac ccggcttacg tgcttgataa gtataagtga actgaaacac
    38401 acggtggcag tcacggcagc gaagtctgtc acggccttta gggttctgac catggcggta
    38461 aacctgagct gactgacaac ggggacaatg aatattaacg ctggtcatga gataacctca
    38521 aaagcccgta ttatacatca gattcaacta attagaggca tcacccatcc agcattataa
    38581 aacatactaa caataactaa attataataa tcacaactta caatcaaata ataaatgcat
    38641 aagattaaca atgatttttt cgggttatat ttatctaaaa aagcagaata ttatgtaact
    38701 tattattttc taagcgaaaa tttattgtgc ggtcagacta acaaacttgc gtaccacccg
    38761 aagaacatgc gaagatttaa gtattctttc ttttttggtt tttatttttc attttctctt
    38821 ttcttctctt tccggttctg ttctggtttt ggttttttcc tttttatttt tattctttct
    38881 tcagggttat gctaaaacac caaaacattt ttcaccacaa aagcatccaa tcatcttgcc
    38941 agaccaatca ccttaaccgg atacactgcc ggatataatc ccactaacac ccagacacca
    39001 agcaccatac ctgataacta ccgtattcag gaaggaaatg cgagtgattt caccgtcacc
    39061 ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca
    39121 atcacttcgt caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc
    39181 agagctttac caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc
    39241 gcgacgaaat cgaccagcgc gttgatgacc gtcagaaccg cggcgtaacg cacaggcagc
    39301 gaggcgggaa gcgccagcac gtacacgtca gttttgggat caagatccag ctgaagcagc
    39361 gcctgttgcc aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg
    39421 cgcagcggca gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt
    39481 gcacccgcgc caatcaccgt ggagcgtacg gtttgcgccg gaaactgcac attcatctca
    39541 cgcaggcgcg gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag
    39601 aacgggttgg cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt
    39661 tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa
    39721 agcgttccgt caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc
    39781 tgcgcgccgg tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc
    39841 tgccccggtt tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca
    39901 ccgacgttga gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg
    39961 gtgccaccgc cgatatcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc
    40021 gccccggctc cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca
    40081 aaatcgccca gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt
    40141 tcggtaaact ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg
    40201 gttttgtcgg ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat
    40261 tcggtgatgg taaaacccag tagatccgcc acttcacggg cttccggggt aataatgctg
    40321 gcgcgcagaa ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc
    40381 gctgtgatga gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac
    40441 cagttgctgc acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc
    40501 cgtctcaagc aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact
    40561 gatgacggcg atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact
    40621 ttccgggtca tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat
    40681 taaggcgtga taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt
    40741 atcattaaat tccacggcca gtgtttttct ggcgagggta taattcccct gcaacgcatt
    40801 aaataacgtt gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc
//
LOCUS       DAJTNV010000028        39814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNV010000028 DAJTNV010000000
VERSION     DAJTNV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            64..225
                     /locus_tag="OO199_001510"
                     /pseudo
     CDS             64..225
                     /locus_tag="OO199_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            454..1044
                     /gene="uidR"
                     /locus_tag="OO199_001511"
     CDS             454..1044
                     /gene="uidR"
                     /locus_tag="OO199_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5535595.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1432..3105
                     /gene="uidA"
                     /locus_tag="OO199_001512"
     CDS             1432..3105
                     /gene="uidA"
                     /locus_tag="OO199_001512"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5535596.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3141..4166
                     /gene="uidB"
                     /locus_tag="OO199_001513"
                     /pseudo
     CDS             3141..4166
                     /gene="uidB"
                     /locus_tag="OO199_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4205..5470
                     /gene="uidC"
                     /locus_tag="OO199_001514"
     CDS             4205..5470
                     /gene="uidC"
                     /locus_tag="OO199_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5535597.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5696..7204)
                     /locus_tag="OO199_001515"
     CDS             complement(5696..7204)
                     /locus_tag="OO199_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5535598.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7305..8480)
                     /gene="manA"
                     /locus_tag="OO199_001516"
     CDS             complement(7305..8480)
                     /gene="manA"
                     /locus_tag="OO199_001516"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5535599.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8679..10325
                     /gene="fumB"
                     /locus_tag="OO199_001517"
     CDS             8679..10325
                     /gene="fumB"
                     /locus_tag="OO199_001517"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5535600.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10468..11871
                     /gene="fumC"
                     /locus_tag="OO199_001518"
     CDS             10468..11871
                     /gene="fumC"
                     /locus_tag="OO199_001518"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5535601.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11868..12797)
                     /gene="tus"
                     /locus_tag="OO199_001519"
     CDS             complement(11868..12797)
                     /gene="tus"
                     /locus_tag="OO199_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5535602.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="OO199_001520"
     CDS             complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="OO199_001520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5535603.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14353..15672)
                     /locus_tag="OO199_001521"
     CDS             complement(14353..15672)
                     /locus_tag="OO199_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5535604.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="OO199_001522"
     CDS             complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="OO199_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5535605.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16769..17104
                     /locus_tag="OO199_001523"
     CDS             16769..17104
                     /locus_tag="OO199_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5535606.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17101..17823)
                     /gene="folM"
                     /locus_tag="OO199_001524"
     CDS             complement(17101..17823)
                     /gene="folM"
                     /locus_tag="OO199_001524"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5535607.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17860..19242)
                     /locus_tag="OO199_001525"
     CDS             complement(17860..19242)
                     /locus_tag="OO199_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5535608.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19428..20372)
                     /locus_tag="OO199_001526"
     CDS             complement(19428..20372)
                     /locus_tag="OO199_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5535609.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20896..22428
                     /gene="pntA"
                     /locus_tag="OO199_001527"
     CDS             20896..22428
                     /gene="pntA"
                     /locus_tag="OO199_001527"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5535610.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22439..23827
                     /gene="pntB"
                     /locus_tag="OO199_001528"
     CDS             22439..23827
                     /gene="pntB"
                     /locus_tag="OO199_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5535611.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="OO199_001529"
     CDS             complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="OO199_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5535612.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25297..25662
                     /gene="mdtJ"
                     /locus_tag="OO199_001530"
     CDS             25297..25662
                     /gene="mdtJ"
                     /locus_tag="OO199_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5535613.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25649..25978
                     /gene="mdtI"
                     /locus_tag="OO199_001531"
     CDS             25649..25978
                     /gene="mdtI"
                     /locus_tag="OO199_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5535614.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(26017..26838)
                     /locus_tag="OO199_001532"
     CDS             complement(26017..26838)
                     /locus_tag="OO199_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5535615.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26938..27021)
                     /locus_tag="OO199_001533"
     CDS             complement(26938..27021)
                     /locus_tag="OO199_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535616.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27114..27449)
                     /locus_tag="OO199_001534"
     CDS             complement(27114..27449)
                     /locus_tag="OO199_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5535617.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(28017..28274)
                     /locus_tag="OO199_001535"
     CDS             complement(28017..28274)
                     /locus_tag="OO199_001535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535618.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28290..29099)
                     /locus_tag="OO199_001536"
     CDS             complement(28290..29099)
                     /locus_tag="OO199_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5535619.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29206..30108
                     /locus_tag="OO199_001537"
     CDS             29206..30108
                     /locus_tag="OO199_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535620.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30243..31463
                     /gene="mlc"
                     /locus_tag="OO199_001538"
                     /gene_synonym="dgsA"
     CDS             30243..31463
                     /gene="mlc"
                     /locus_tag="OO199_001538"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5535621.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31588..32283
                     /gene="bioD"
                     /locus_tag="OO199_001539"
     CDS             31588..32283
                     /gene="bioD"
                     /locus_tag="OO199_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5535622.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="OO199_001540"
     CDS             complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="OO199_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5535623.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="OO199_001541"
     CDS             complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="OO199_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5535624.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34344..35198)
                     /locus_tag="OO199_001542"
     CDS             complement(34344..35198)
                     /locus_tag="OO199_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5535625.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="OO199_001543"
     CDS             complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="OO199_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5535626.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35828..37866)
                     /locus_tag="OO199_001544"
                     /pseudo
     CDS             complement(35828..37866)
                     /locus_tag="OO199_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37927..39036)
                     /locus_tag="OO199_001545"
     CDS             complement(37927..39036)
                     /locus_tag="OO199_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5535627.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            39019..>39814
                     /locus_tag="OO199_001546"
     CDS             39019..>39814
                     /locus_tag="OO199_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5535628.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10144 a         9649 c        10187 g         9834 t
ORIGIN      
        1 gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat
       61 tacccagaaa ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg
      121 caagatattg ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga
      181 caaattctca ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg
      241 gtaaacggtc gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct
      301 aaacaactgg cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg
      361 aatagagtgt accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt
      421 aaactagtta ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca
      481 caaccgacac ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt
      541 cacagtgcct cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat
      601 caccatttca tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg
      661 gcgctggctc gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag
      721 tctattgtct ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg
      781 gcggaaggga tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc
      841 actgaatttg ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac
      901 atcaacacgg caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat
      961 atcgaagcgg aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt
     1021 ggcggtatat taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg
     1081 cgtttcatat cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc
     1141 cccaaaatat tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac
     1201 gaacgttcga ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc
     1261 catatcacgc tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta
     1321 tttccatttc tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa
     1381 ctaatcagat taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt
     1441 cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg
     1501 gatcgcgaaa actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg
     1561 gcaattgcag tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat
     1621 gtgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt
     1681 atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa
     1741 gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt
     1801 gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc
     1861 ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat
     1921 ttctttaact atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg
     1981 gtggacgata tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac
     2041 tggcaggtgg tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg
     2101 gttgcaactg gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa
     2161 ccgggtgaag gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat
     2221 atctacccgc tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt
     2281 aaccacaaac cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc
     2341 aaaggattcg ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc
     2401 aactcctacc gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa
     2461 catggcatcg tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt
     2521 ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa
     2581 actcagcagg cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca
     2641 agcgtggtga tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa
     2701 tatttcgcgc cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc
     2761 gtcaatgtaa tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg
     2821 tgcctgaacc gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag
     2881 gtactggaaa aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc
     2941 gaatacggcg tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa
     3001 gagtatcagt gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc
     3061 gtcggtgaac aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt
     3121 ggcggtaaca attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt
     3181 atggttcact tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg
     3241 ctcgtgggat tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca
     3301 ttaagaactc cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg
     3361 tgattgccgg aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac
     3421 gtatcgtggc gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc
     3481 tgtttatgtt gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct
     3541 cgtcgttgtt ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac
     3601 tggtgcaaaa cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga
     3661 ggatcggtaa aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc
     3721 tattcttctg ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt
     3781 caattggtca gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat
     3841 acggtgaata cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta
     3901 cccgtaaatg cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg
     3961 gatatatcgc caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg
     4021 ccttagtacc ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca
     4081 cggataaaaa attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc
     4141 aattaatcag cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc
     4201 aactatgaga aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac
     4261 ctctgcttac gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa
     4321 cgaattgcgc agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt
     4381 acagggagga ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg
     4441 cggagcgtat tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct
     4501 tgatggtgat aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa
     4561 tagcctgctt aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg
     4621 tattccgtta atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt
     4681 aggttattgg gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt
     4741 tttatggact gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg
     4801 aaaatacgat aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag
     4861 tcgctattct ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct
     4921 cgagaaaagc ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta
     4981 cgcgcagctc gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag
     5041 tggacaattg acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg
     5101 ccatgcaatg aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct
     5161 tgatcgtaac cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc
     5221 gcaatttacg ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga
     5281 tgtgcggatt gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc
     5341 gttacaaggg atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag
     5401 tacgctgggc gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat
     5461 gctgaagtaa gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag
     5521 tgcgcgtttt ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa
     5581 attcaaagtg ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg
     5641 tttgttttca gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact
     5701 gctgcggaat tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa
     5761 aatcttctaa cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc
     5821 tggtggtgat ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg
     5881 caccttcaac ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct
     5941 tcgctacctg agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca
     6001 gagatttaac cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg
     6061 ccggatcttt caggaacagc gacagattca gcgcactttc accctgactg tttttccagc
     6121 ttagcggcgc gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga
     6181 aggcttccgt cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca
     6241 gcagcgcctg agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa
     6301 tctggccgac ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc
     6361 tgtttagcga gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt
     6421 taccgctgat ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca
     6481 ttttttccag tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg
     6541 aaccgtcggt tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg
     6601 cgtctatccg accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag
     6661 catttaactg gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca
     6721 gtggattgag cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct
     6781 caaaaggcgt ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca
     6841 gcgtggtttg aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg
     6901 ggaagggacc atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg
     6961 gattttcttt cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc
     7021 gatgatagtt ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg
     7081 cgttcgcctg cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg
     7141 cgccgcctgt ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat
     7201 tcataatgat tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc
     7261 ctttaataag cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa
     7321 acacgcgcta aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac
     7381 gctgattcac ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct
     7441 tcgacgcaga acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg
     7501 tcatgcagcg agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt
     7561 ttcaccggct gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc
     7621 agttccggaa tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc
     7681 gccatcactt ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac
     7741 atcgcttcgc caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg
     7801 tcttccgggt aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg
     7861 tcgagcgccg attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac
     7921 aggttggcga acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc
     7981 gccggatgtg caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac
     8041 gcgttcatcg caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct
     8101 ttatagttac gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg
     8161 atttcagaat tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat
     8221 actttgaaca ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc
     8281 gatttatcac tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact
     8341 cgtgaactgc ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga
     8401 ttttccatac cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact
     8461 gagttaatga gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt
     8521 aaagcaagaa tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt
     8581 ttgttaaaaa actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc
     8641 ccagagcata accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt
     8701 atcagtctcc ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac
     8761 acgttagcgt atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa
     8821 ctctgttggc gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac
     8881 aacaggtggc cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc
     8941 tgcaattcct gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata
     9001 ccggcaccgc gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg
     9061 aagcggcgct ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc
     9121 aaaacgcgcc gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga
     9181 tcgatcttta tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg
     9241 gttcggcaaa caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac
     9301 tgaaaaatta cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc
     9361 atattgcgtt cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg
     9421 cttccgcgaa atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc
     9481 gcgatgtgga actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc
     9541 agtttggtgg taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg
     9601 catcctgtcc ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga
     9661 tcaaccgtca ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg
     9721 aagagctgcg taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga
     9781 aagagatcct cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca
     9841 cgattatcgt tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg
     9901 aagggctgcc gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc
     9961 cggaaggtta tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg
    10021 tcgatcaact gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc
    10081 agcaggtgac ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc
    10141 cggccgctgt attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac
    10201 tgggaatgga agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg
    10261 atgataaagg aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga
    10321 aataaacaga gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg
    10381 ttatcaaata gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga
    10441 aaaaattaat caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg
    10501 ggggcgattg atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag
    10561 catttccgca tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc
    10621 aagcgcgcag cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc
    10681 gccattcggc aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg
    10741 gctatctggc agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct
    10801 aaccgggcca gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac
    10861 gacgacgtga acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg
    10921 gcgctgctgg cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg
    10981 agtgaaaaat cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac
    11041 gccacgccgc taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat
    11101 ctcaaacata tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg
    11161 gtgggtactg gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca
    11221 gtcattacct gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt
    11281 gatgccctgg ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc
    11341 gccaatgatg tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc
    11401 ccggaaaatg agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa
    11461 gcattaacca tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc
    11521 gcttccggta actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa
    11581 tcggtgcgct tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt
    11641 gaaccgaatc gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg
    11701 cttaacaccc acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa
    11761 gggctgacct taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac
    11821 agctgggtac ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca
    11881 tacaggtgca gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc
    11941 tgcacattgt cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta
    12001 tcccgattaa tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct
    12061 cctttgtacc agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc
    12121 gcgttctgtg gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc
    12181 tcgcgcgtcc acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg
    12241 acttcatcac gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg
    12301 gcggggtcgt gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa
    12361 tgacgatgca cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata
    12421 tgctcgaacg tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc
    12481 gaaaggttat cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg
    12541 cgattttcgg actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc
    12601 gcgtcgttgc cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct
    12661 ttttttacct ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga
    12721 gcggcaaatg tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg
    12781 agatcgtaac gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca
    12841 gtcgtggtgg cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta
    12901 tgccataacg gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta
    12961 accgtaccgc ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg
    13021 ccggttgagc gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg
    13081 ttttctgggg caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac
    13141 agtagcaggc tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta
    13201 tccagcacgc gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt
    13261 tttatccgta ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc
    13321 cacaacggca ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca
    13381 taagtcagca gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg
    13441 gattcggcgg cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt
    13501 gttcgcagtt cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg
    13561 atattgtccg ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca
    13621 aagtggatac gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt
    13681 aacatatcct gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca
    13741 ataaaagcga tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga
    13801 acaggaccaa ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga
    13861 tcgtccaggg cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg
    13921 tatttactca gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc
    13981 gtcttacccc agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa
    14041 ctgttcatca aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc
    14101 agcccaacca gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata
    14161 aacaattttt tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt
    14221 ggagcaatgt aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga
    14281 cggatgtatc cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta
    14341 ttatgctaag gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag
    14401 aagacgggct gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc
    14461 catatcaacg atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag
    14521 tgttgaaagg acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat
    14581 tgagccaagc gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat
    14641 acccattgtt ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt
    14701 ttgtcccatg ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa
    14761 tttttccaat gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc
    14821 aaaggtaagt acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag
    14881 caaaatgccc gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa
    14941 cccgagcaaa ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc
    15001 cattacccca taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg
    15061 aatcgcgttg atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt
    15121 taacagcgca ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct
    15181 tgccccggtc agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa
    15241 agataccgca gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa
    15301 ggcgctggca tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat
    15361 gactaaacac aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt
    15421 ttcgagctta ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt
    15481 taataaatta acataaccgt taccagcaat attataccat tcgataacgg tttcactgct
    15541 tgtctcagga atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa
    15601 aacacttttg gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat
    15661 caaaaaaagc acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca
    15721 gcacctgaaa tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg
    15781 aggcgattat agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg
    15841 aataagctct tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag
    15901 tcatctgatt gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta
    15961 cgcacagttt taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc
    16021 gaaatagcca cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc
    16081 agcaatgcat cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg
    16141 aaatcagctg tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg
    16201 tcgatagtta acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga
    16261 cctttggtca gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa
    16321 acagcagggg gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg
    16381 tggttcatat cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt
    16441 aaatcacgac aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa
    16501 tccggatttt ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc
    16561 tgcatatcat gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc
    16621 acaaatacga tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg
    16681 ctgtttttac ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga
    16741 cttgttatgg aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc
    16801 gctggtagtg ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct
    16861 gattccactt ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg
    16921 cattgaagcc ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt
    16981 ttacctggtg tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg
    17041 ttctgttgcg tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca
    17101 ttaacgcaga tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt
    17161 aagtaagtaa tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga
    17221 tttattcagc gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga
    17281 tggcgcaata gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat
    17341 attatccagt gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac
    17401 cacataatcg gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct
    17461 ttccagcgca tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag
    17521 tacgtcggcc agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat
    17581 agcacgcaga ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt
    17641 ggtcgaaaaa tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg
    17701 atagtgtgtc cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag
    17761 ggcgaggccg atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc
    17821 catcgttatc tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt
    17881 cgccggaact gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac
    17941 attatcatgt gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag
    18001 aacaacggat aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat
    18061 gcaggcaccg acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc
    18121 gccgaccagg aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta
    18181 atcggaaccg gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca
    18241 cagtgaggcg gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt
    18301 atgagtggca gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc
    18361 gcaaacggaa acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc
    18421 caccatcaga ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac
    18481 acccagcgaa agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag
    18541 tgtcgctttg ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc
    18601 cacaccaatg aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg
    18661 tacgccaagt gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat
    18721 cgccagcaca acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat
    18781 gctggcagcg gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga
    18841 tgcaccgact atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc
    18901 cgtaaaaaag cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc
    18961 gcacagccag tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc
    19021 ataggtaaag ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa
    19081 ggccagcaat aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct
    19141 ggcaactgcc gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa
    19201 taccagcgcg gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa
    19261 acgtgataag aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag
    19321 cgcgggattt tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa
    19381 aaatgcgaaa ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga
    19441 tctgcgctga cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac
    19501 ttagcacctt tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg
    19561 tagttgccag agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc
    19621 agttcttcga ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac
    19681 tgggtttcaa agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg
    19741 atctcaactt tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa
    19801 tctgccggga tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg
    19861 aatgcagtaa tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa
    19921 gagtaggcac ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta
    19981 cgatagaagc cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc
    20041 ggcagttcga caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg
    20101 gctttataga ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg
    20161 acaacataaa aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct
    20221 tcgccaatag cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc
    20281 gcttgctccg gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt
    20341 gccgacgcca ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc
    20401 aggtactggt attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac
    20461 tgtctgcgtc atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc
    20521 gtctatgctt aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata
    20581 agttaattta atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt
    20641 cgaagacaat tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg
    20701 aatcgttaat attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc
    20761 ataattttca gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta
    20821 gctcgtacat gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg
    20881 atggaaggga atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt
    20941 gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta
    21001 gagagcggcg cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct
    21061 gaaattgtag aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg
    21121 ttagatgatg aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct
    21181 gcgcagaatc cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg
    21241 gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat
    21301 atcgccggtt atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg
    21361 caaatcactg cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt
    21421 gcaggtttgg ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac
    21481 acccgcccgg aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat
    21541 tttaaagagg aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc
    21601 aaagcggaaa tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc
    21661 gcgcttattc caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg
    21721 aaggcgggca gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc
    21781 gtgccgggtg aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt
    21841 ccgggccgtc tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg
    21901 aaactgttgt gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt
    21961 cgcggcgtga ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta
    22021 tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt
    22081 gcctgctcac cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg
    22141 ctgtcaagcg ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc
    22201 gttgtcggtt attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg
    22261 gttaccaacg cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga
    22321 ggctgggtta gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt
    22381 ggcttcaccg tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat
    22441 gtctggagga ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct
    22501 ggccggtctt tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat
    22561 ggcgattgcg ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt
    22621 gctggcgatg gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac
    22681 cgaaatgcca gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt
    22741 tggctttaac agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca
    22801 cctgacggaa gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt
    22861 ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca
    22921 caaaatgaac ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac
    22981 ggacagcgtc ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt
    23041 cggctggcat ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct
    23101 gaactcgtac tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct
    23161 gattgtgacc ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa
    23221 ggcgatgaac cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc
    23281 tactggcgat gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga
    23341 actgctgaaa aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca
    23401 ggcgcaatat cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg
    23461 tttcggtatc cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga
    23521 agcaaaagta ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga
    23581 taccgatacc gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga
    23641 tccgaagagt ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat
    23701 tgtctttaaa cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa
    23761 ggaaaacacc cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc
    23821 tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa
    23881 cggcgatgct ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg
    23941 gcacggaaag cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa
    24001 ataccaccaa tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc
    24061 cgaagaccag attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat
    24121 taaacaccag tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga
    24181 tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg
    24241 cgagcatcgc ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat
    24301 aatgagaaac actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca
    24361 tcatttgctg aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca
    24421 gcagcaataa aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca
    24481 acgtcatcgc cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc
    24541 ctacgcgttg caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt
    24601 gcggtaacgt ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca
    24661 tcgccatcac gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc
    24721 ggaccatatg ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg
    24781 gcacgatgat ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca
    24841 taatgacgat ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt
    24901 ttcttccttt gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg
    24961 ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg
    25021 tgttcaacta ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt
    25081 gtaatattat ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct
    25141 catccactat cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc
    25201 tgcctaagct tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag
    25261 cgaaaattaa aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta
    25321 ggtctggcta ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt
    25381 gagggaaatg gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc
    25441 tctttcgccg ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt
    25501 attttattta ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa
    25561 attgccgggt taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa
    25621 gcgcgtaaac ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg
    25681 gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg
    25741 ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc
    25801 gctttctcag gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt
    25861 tggtattgcc gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa
    25921 aggctggatt ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg
    25981 aagacgctgc ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag
    26041 cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc
    26101 ggcaggagcc gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag
    26161 aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc
    26221 ccagccagtc acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta
    26281 tcccgcctga gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc
    26341 tccctcaaat aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc
    26401 gaagtcccac ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg
    26461 ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc
    26521 tttatttgac acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa
    26581 taaacagtgt cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt
    26641 gccgctggcc gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac
    26701 tggcacacga tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc
    26761 aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac
    26821 agcaatggtt gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc
    26881 tcataaatac tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca
    26941 ggaaagataa aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc
    27001 aaagcgataa cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct
    27061 tagcaccgta attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct
    27121 gcgggttgtg cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc
    27181 gctgcctgcg ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt
    27241 ttagccgcct gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt
    27301 ttcgctggcg ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca
    27361 gcaaaggcgg cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc
    27421 attgtcatac cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact
    27481 atatccctgg gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac
    27541 aaacgctgaa taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct
    27601 accaataata gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa
    27661 ctgcgcatca gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa
    27721 tcctgatgac gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc
    27781 tattaacgtt ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt
    27841 atttttcagg cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc
    27901 atcagagcaa taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc
    27961 agcgtcccgg caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag
    28021 cctttagcgc gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag
    28081 aatccggctg agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc
    28141 atcagtaaac caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag
    28201 cgggtggtca tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat
    28261 aataagccaa ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg
    28321 taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt
    28381 aatccccggt cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa
    28441 gtcgggcaaa aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc
    28501 gcgaaacaac cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca
    28561 ctaattaagc gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa
    28621 aaggccacga aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg
    28681 ccacttaacg aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc
    28741 attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg
    28801 cgaccaatgg catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg
    28861 gaaatggaca gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc
    28921 ggaaggatag gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc
    28981 gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa
    29041 atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact
    29101 tgaaatcctt gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat
    29161 tgttctgtct aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt
    29221 cgtcatctgc gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc
    29281 cgcctgaata tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc
    29341 ggtgcccgac tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag
    29401 tttcttgcag atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa
    29461 agactgcatc agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt
    29521 attcgggcgg tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta
    29581 caaacccgcg aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat
    29641 atgggattgc tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat
    29701 gaaccgctta tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca
    29761 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
    29821 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
    29881 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
    29941 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
    30001 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
    30061 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
    30121 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
    30181 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
    30241 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
    30301 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
    30361 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
    30421 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
    30481 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
    30541 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
    30601 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
    30661 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
    30721 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
    30781 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
    30841 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
    30901 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
    30961 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
    31021 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
    31081 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
    31141 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
    31201 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
    31261 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
    31321 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
    31381 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
    31441 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
    31501 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
    31561 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
    31621 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
    31681 aaaagggttg cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg
    31741 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
    31801 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
    31861 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
    31921 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
    31981 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
    32041 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
    32101 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
    32161 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
    32221 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
    32281 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
    32341 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
    32401 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
    32461 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
    32521 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
    32581 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
    32641 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
    32701 cccgactagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
    32761 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
    32821 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
    32881 gtcgctatga ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg
    32941 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
    33001 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
    33061 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
    33121 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
    33181 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
    33241 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
    33301 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc
    33361 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
    33421 atgacgaaac ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag
    33481 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
    33541 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
    33601 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
    33661 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
    33721 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
    33781 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
    33841 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
    33901 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
    33961 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
    34021 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
    34081 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
    34141 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
    34201 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
    34261 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
    34321 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
    34381 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
    34441 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
    34501 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
    34561 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
    34621 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
    34681 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
    34741 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
    34801 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
    34861 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
    34921 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
    34981 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
    35041 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
    35101 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
    35161 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
    35221 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
    35281 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
    35341 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
    35401 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
    35461 ttcagcattg tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat
    35521 acaaacatct tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg
    35581 gcagactttt gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc
    35641 gaacacgttc tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata
    35701 aatacggcgg aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca
    35761 ggttttacag ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg
    35821 ctactcctta aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca
    35881 gcggtgaagg gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac
    35941 caaacatatc cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac
    36001 gcggagtcac ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat
    36061 cgccatgacg gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc
    36121 aggcctgctg taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc
    36181 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
    36241 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
    36301 tgttggcatc aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
    36361 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
    36421 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
    36481 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg
    36541 gtcgatggca tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat
    36601 cgggaaccat tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt
    36661 tatgccgacg ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc
    36721 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
    36781 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
    36841 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
    36901 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
    36961 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
    37021 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
    37081 gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    37141 agtgacacca ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag
    37201 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    37261 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga
    37321 gttcatcaga cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc
    37381 gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact
    37441 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc
    37501 gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg
    37561 cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga
    37621 ctccacccag tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca
    37681 gttaacggtg caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac
    37741 catttgggaa aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga
    37801 ggttttcatc agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat
    37861 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    37921 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    37981 tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc
    38041 agacatattg ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg
    38101 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    38161 gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca
    38221 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    38281 accaaacagt tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga
    38341 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    38401 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    38461 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt
    38521 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    38581 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata
    38641 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc
    38701 gaatttttcg ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc
    38761 gttaggaata atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata
    38821 ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc
    38881 ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc
    38941 gtagttccag atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac
    39001 gctacttaag gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt
    39061 tctttctctg ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg
    39121 ctgacacaaa tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac
    39181 actgtccgct tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc
    39241 acagaccgga ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc
    39301 ctactggaca atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct
    39361 gagcagttcc tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg
    39421 gttgacgaga agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag
    39481 cagaggggca ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact
    39541 ggtgatacct ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat
    39601 aataaccata ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag
    39661 ggggatagac cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa
    39721 tcatctgtac tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg
    39781 aaacaatcca gctttaagga cttgtcagcg tccc
//
LOCUS       DAJTNV010000029        38776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNV010000029 DAJTNV010000000
VERSION     DAJTNV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO199_001547"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO199_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5535629.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO199_001548"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO199_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5535630.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO199_001549"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO199_001549"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5535631.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO199_001550"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO199_001550"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5535632.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO199_001551"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO199_001551"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5535633.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO199_001552"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO199_001552"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5535634.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO199_001553"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO199_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5535635.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO199_001554"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO199_001554"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5535636.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO199_001555"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO199_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5535637.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO199_001556"
     CDS             7139..7750
                     /locus_tag="OO199_001556"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5535638.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO199_001557"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO199_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5535639.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO199_001558"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO199_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO199_001559"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO199_001559"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5535640.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO199_001560"
     CDS             complement(11944..12303)
                     /locus_tag="OO199_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535641.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12806)
                     /locus_tag="OO199_001561"
     CDS             complement(12300..12806)
                     /locus_tag="OO199_001561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535642.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="OO199_001562"
     CDS             complement(12952..13515)
                     /locus_tag="OO199_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535643.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="OO199_001563"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="OO199_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="OO199_001564"
     CDS             complement(14723..15097)
                     /locus_tag="OO199_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5535644.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO199_001565"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="OO199_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5535645.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLFLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO199_001566"
     CDS             complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="OO199_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5535646.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="OO199_001567"
     CDS             complement(17167..18057)
                     /locus_tag="OO199_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5535647.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO199_001568"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="OO199_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5535648.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO199_001569"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="OO199_001569"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5535649.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO199_001570"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="OO199_001570"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5535650.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO199_001571"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="OO199_001571"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5535651.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO199_001572"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="OO199_001572"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5535652.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO199_001573"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="OO199_001573"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5535653.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO199_001574"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="OO199_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5535654.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO199_001575"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="OO199_001575"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5535655.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO199_001576"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="OO199_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5535656.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO199_001577"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="OO199_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5535657.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO199_001578"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="OO199_001578"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5535658.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO199_001579"
                     /gene_synonym="manB"
     CDS             complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="OO199_001579"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5535659.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO199_001580"
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="OO199_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5535660.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO199_001581"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="OO199_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5535661.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO199_001582"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="OO199_001582"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5535662.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36941)
                     /gene="fcl"
                     /locus_tag="OO199_001583"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35983..36941)
                     /gene="fcl"
                     /locus_tag="OO199_001583"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36944..38065)
                     /gene="gmd"
                     /locus_tag="OO199_001584"
     CDS             complement(36944..38065)
                     /gene="gmd"
                     /locus_tag="OO199_001584"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5535663.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38091..38639)
                     /gene="wcaF"
                     /locus_tag="OO199_001585"
     CDS             complement(38091..38639)
                     /gene="wcaF"
                     /locus_tag="OO199_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5535664.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38655..>38776)
                     /locus_tag="OO199_001586"
     CDS             complement(38655..>38776)
                     /locus_tag="OO199_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5535665.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10358 a         9709 c         8722 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctagccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggaccaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgaggtatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca gtttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaccc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaaaca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 attttataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaata ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtaagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tccagcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagca ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgattc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaacaacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgcg cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgagggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gctgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca agtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccac
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc accagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gctctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcagccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga gttactcggg
    36421 tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta atcgcgtcca
    36481 tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc
    36541 tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc
    36601 agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc gttctgatgc
    36661 gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag
    36721 gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt
    36781 tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc atcgcgggta
    36841 cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc agaaccgacc
    36901 atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga
    36961 tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat
    37021 tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt
    37081 cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg
    37141 gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg
    37201 aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct
    37261 gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc gcgataacga
    37321 aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt
    37381 ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg
    37441 cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg
    37501 attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa
    37561 cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg
    37621 tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat
    37681 agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac
    37741 ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca
    37801 ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag
    37861 aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct
    37921 gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca
    37981 cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac
    38041 cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt
    38101 tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc
    38161 gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg
    38221 gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta
    38281 atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat
    38341 atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgcca
    38401 acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac tgacggacga
    38461 ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga
    38521 tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact
    38581 ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt
    38641 ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt cgtacgttgg
    38701 cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg ccgttggact
    38761 tttttggcgt cagcac
//
LOCUS       DAJTNV010000030        37562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNV010000030 DAJTNV010000000
VERSION     DAJTNV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..175)
                     /locus_tag="OO199_001587"
     CDS             complement(<1..175)
                     /locus_tag="OO199_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5535666.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO199_001588"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO199_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5535667.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO199_001589"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO199_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5535668.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO199_001590"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO199_001590"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5535669.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO199_001591"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO199_001591"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5535670.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO199_001592"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO199_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5535671.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO199_001593"
     CDS             complement(5179..5421)
                     /locus_tag="OO199_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5535672.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO199_001594"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO199_001594"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5535673.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO199_001595"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO199_001595"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5535674.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO199_001596"
     CDS             complement(8338..9312)
                     /locus_tag="OO199_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5535675.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO199_001597"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO199_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5535676.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO199_001598"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO199_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5535677.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO199_001599"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO199_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5535678.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO199_001600"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO199_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5535679.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO199_001601"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO199_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5535680.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO199_001602"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO199_001602"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5535681.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO199_001603"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO199_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5535682.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO199_001604"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO199_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5535683.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO199_001605"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO199_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5535684.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO199_001606"
     CDS             23179..23382
                     /locus_tag="OO199_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5535685.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO199_001607"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO199_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5535686.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO199_001608"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO199_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5535687.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO199_001609"
     CDS             26387..26659
                     /locus_tag="OO199_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5535688.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO199_001610"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO199_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5535689.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO199_001611"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO199_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5535690.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO199_001612"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO199_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5535691.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO199_001613"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO199_001613"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5535692.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO199_001614"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO199_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO199_001615"
     CDS             complement(31906..32304)
                     /locus_tag="OO199_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5535693.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO199_001616"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO199_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5535694.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO199_001617"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO199_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5535695.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO199_001618"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO199_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5535696.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO199_001619"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO199_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5535697.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO199_001620"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO199_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5535698.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..>37562)
                     /gene="dcuC"
                     /locus_tag="OO199_001621"
     CDS             complement(36260..>37562)
                     /gene="dcuC"
                     /locus_tag="OO199_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5535699.1"
                     /translation="KPQVVLAAAGVFLMMCGVWLGFGGVLDPAKSSGYLIVDIYNEIL
                     RMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSRPLKLIRSPYIILSATYVIG
                     QIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAA
                     QVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVP
                     PLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRET
                     MDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMII
                     MALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPIT
                     AVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9090 a         9406 c         9887 g         9179 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccataccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgtttgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg tctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 aggcaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 taggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acagcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttccccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgt tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tg
//
LOCUS       DAJTNV010000031        36833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNV010000031 DAJTNV010000000
VERSION     DAJTNV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..245
                     /locus_tag="OO199_001622"
     CDS             <1..245
                     /locus_tag="OO199_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5535700.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO199_001623"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO199_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5535701.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO199_001624"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO199_001624"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5535702.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="OO199_001625"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="OO199_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5535703.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3540)
                     /locus_tag="OO199_001626"
     CDS             complement(3256..3540)
                     /locus_tag="OO199_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535704.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="OO199_001627"
     CDS             complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="OO199_001627"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5535705.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4441..5649)
                     /locus_tag="OO199_001628"
     CDS             complement(4441..5649)
                     /locus_tag="OO199_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5535706.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5733..6269
                     /gene="rcdA"
                     /locus_tag="OO199_001629"
     CDS             5733..6269
                     /gene="rcdA"
                     /locus_tag="OO199_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5535707.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6444..8129)
                     /locus_tag="OO199_001630"
     CDS             complement(6444..8129)
                     /locus_tag="OO199_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5535708.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8399..8776
                     /locus_tag="OO199_001631"
     CDS             8399..8776
                     /locus_tag="OO199_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5535709.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="OO199_001632"
     CDS             complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="OO199_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5535710.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9223..9510
                     /locus_tag="OO199_001633"
     CDS             9223..9510
                     /locus_tag="OO199_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5535711.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9494..10216
                     /gene="nfsA"
                     /locus_tag="OO199_001634"
     CDS             9494..10216
                     /gene="nfsA"
                     /locus_tag="OO199_001634"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5535712.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10277..11179
                     /gene="rimK"
                     /locus_tag="OO199_001635"
     CDS             10277..11179
                     /gene="rimK"
                     /locus_tag="OO199_001635"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5535713.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11267..11743
                     /locus_tag="OO199_001636"
     CDS             11267..11743
                     /locus_tag="OO199_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5535714.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12095..13207
                     /gene="potF"
                     /locus_tag="OO199_001637"
     CDS             12095..13207
                     /gene="potF"
                     /locus_tag="OO199_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5535715.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13302..14435
                     /gene="potG"
                     /locus_tag="OO199_001638"
     CDS             13302..14435
                     /gene="potG"
                     /locus_tag="OO199_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5535716.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14445..15398
                     /gene="potH"
                     /locus_tag="OO199_001639"
     CDS             14445..15398
                     /gene="potH"
                     /locus_tag="OO199_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5535717.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15395..16240
                     /gene="potI"
                     /locus_tag="OO199_001640"
     CDS             15395..16240
                     /gene="potI"
                     /locus_tag="OO199_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5535718.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16300..16788
                     /locus_tag="OO199_001641"
     CDS             16300..16788
                     /locus_tag="OO199_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5535719.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16829..17956
                     /gene="rlmC"
                     /locus_tag="OO199_001642"
     CDS             16829..17956
                     /gene="rlmC"
                     /locus_tag="OO199_001642"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5535720.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18131..18862)
                     /gene="artJ"
                     /locus_tag="OO199_001643"
     CDS             complement(18131..18862)
                     /gene="artJ"
                     /locus_tag="OO199_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5535721.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19154..19822)
                     /gene="artM"
                     /locus_tag="OO199_001644"
     CDS             complement(19154..19822)
                     /gene="artM"
                     /locus_tag="OO199_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5535722.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19822..20538)
                     /gene="artQ"
                     /locus_tag="OO199_001645"
     CDS             complement(19822..20538)
                     /gene="artQ"
                     /locus_tag="OO199_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5535723.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20545..21276)
                     /gene="artJ"
                     /locus_tag="OO199_001646"
     CDS             complement(20545..21276)
                     /gene="artJ"
                     /locus_tag="OO199_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5535724.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21294..22022)
                     /gene="artP"
                     /locus_tag="OO199_001647"
     CDS             complement(21294..22022)
                     /gene="artP"
                     /locus_tag="OO199_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5535725.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22240..22755)
                     /locus_tag="OO199_001648"
     CDS             complement(22240..22755)
                     /locus_tag="OO199_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5535726.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23404..25173
                     /locus_tag="OO199_001649"
     CDS             23404..25173
                     /locus_tag="OO199_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5535727.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25384..25707
                     /locus_tag="OO199_001650"
     CDS             25384..25707
                     /locus_tag="OO199_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5535728.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25704..26534
                     /gene="amiD"
                     /locus_tag="OO199_001651"
     CDS             25704..26534
                     /gene="amiD"
                     /locus_tag="OO199_001651"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5535729.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26531..27544)
                     /locus_tag="OO199_001652"
     CDS             complement(26531..27544)
                     /locus_tag="OO199_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5535730.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27643..29073)
                     /locus_tag="OO199_001653"
     CDS             complement(27643..29073)
                     /locus_tag="OO199_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5535731.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29084..30085)
                     /gene="ltaE"
                     /locus_tag="OO199_001654"
     CDS             complement(29084..30085)
                     /gene="ltaE"
                     /locus_tag="OO199_001654"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5535732.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30122..31840)
                     /gene="poxB"
                     /locus_tag="OO199_001655"
     CDS             complement(30122..31840)
                     /gene="poxB"
                     /locus_tag="OO199_001655"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5535733.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31973..32941)
                     /gene="hcr"
                     /locus_tag="OO199_001656"
     CDS             complement(31973..32941)
                     /gene="hcr"
                     /locus_tag="OO199_001656"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5535734.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32953..34605)
                     /gene="hcp"
                     /locus_tag="OO199_001657"
                     /gene_synonym="priS"
     CDS             complement(32953..34605)
                     /gene="hcp"
                     /locus_tag="OO199_001657"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5535735.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34749..35648)
                     /gene="lysO"
                     /locus_tag="OO199_001658"
     CDS             complement(34749..35648)
                     /gene="lysO"
                     /locus_tag="OO199_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5535736.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36105..36800)
                     /gene="aqpZ"
                     /locus_tag="OO199_001659"
     CDS             complement(36105..36800)
                     /gene="aqpZ"
                     /locus_tag="OO199_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5535737.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9215 a         9156 c         9441 g         9021 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata atccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccctgtt gcgcttcttg cagggcataa
     2341 gcttctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggcga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccatgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcgcagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa cgctgactga
     3421 atcaggaacc acaaccactt tgtatccact cggcacatcg acggtgacac tttgcgccat
     3481 cgccacacca gtaaagccca ttaaaagcgc gcccaacagt agacaatttt tcataatcat
     3541 ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta tgcagccttc attcaggcat
     3601 tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt
     3661 tatcgatcac atccagtacg ccttcacggt tattgtagcc tgcccggtat tttgccgctg
     3721 cgacgactgc gctgccggca ttttccattg caaaactaaa gcctgcctga cgcagcatct
     3781 caatatcgtt accgccatcg ccaaagacca ccacttcgct gtcgtctatt ccccataatt
     3841 tctgcagttg gcgaaggcca ttggctttat gtacgccggg gataatcaga tcgatgctgc
     3901 cgttgccggt gtggactggc accataatat cgccgatggc ctcatgtaat gctttttgta
     3961 cttgtggaat cagttcatcg gaaagattca ggccaaactt aaagaaaata tcttctaagt
     4021 tgtcaaagtt atcgacgtat tccagacggt gataatacat ttccgccacc gttttcatgg
     4081 catcgtcata ttttttgagt gtataggcgc tattttttcc gcaggcaata atttccactt
     4141 ccgggcgcgt cagcaaatgt tccacgacag tagcaaacgc atccttcgac agctcgccat
     4201 taaaaacatc tttgccttca ctcattaccc agccgccgtt ttcagccaca aaggcaattt
     4261 cattcgcaat ttcagggaag aaagagatca actgataata ttgattcccg ctggcgacca
     4321 caaagcgaat gccttgtgct ttcatttgct gatactgagc cataaaccgc tcacggttat
     4381 aggttttttg atcgcttaag aaagtaccat ccatgtctac cgcaattaat ttaatgctca
     4441 tcaactattc tccatcgccg tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac
     4501 aatagccgcg agaataacca gcgccagtac aaccagcatt gcactacgta atccataatg
     4561 ttcgccgaga tagcccagca gcggcggccc gacgaggaaa gccagataac cggtcgttgc
     4621 taccacactg acgcgggttg gtgcatcggg gccggtatcg ctggcggcag aaatggtcag
     4681 cgggaagccc agcgaggcac ccagtcccca aagtacaaca gacactcctg cgacccaggc
     4741 gctatcgaca aaaataatca gcccaatacc caacgccccc attagtgcac tggcccgaac
     4801 cacggcaacg cgactgtaac tgtcgatgaa ccaaccgccg gtgaagcgtc caacggtcat
     4861 ccccagggta aaaccggcat aaatcagtga gccggaagtg gggctaaaac cgtgaccatc
     4921 aaccattaat aagggtaacc agtcgttggc agaaccttcg gcaaaggcca tcgccagcac
     4981 tacaacacca atcaacagca actggatatc gcgataaaaa ggaatgcctt tttcgccatg
     5041 ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg attgcctgaa tggcgatata
     5101 aataggcgcg atacctacca gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa
     5161 ggccgtcagt gccatcccga caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg
     5221 catcatcggc aaaaccgttt tattcatttc tcgctcaacg gcggcacctt caacgtttat
     5281 cgccacttcc gcagaaccaa aacttgcccc aaagacgccg agaccaacgg caaagagcag
     5341 gggcgatgtc agccagagtg ccagacttaa tatcatcatc ccgatcaatg cgcaggacat
     5401 cgtgaccagg atgacattac gtgtcccaaa gcgtttcact aaccacgccg agcagagaat
     5461 accgctcatc gaaccgatcg acagaccaaa gagaacaccg cccatttcag cgatcgagac
     5521 agagagaata tcgcggatag caggcgtacg ggttgcccag gacgccatta acaggcctgg
     5581 caaaaagaag aacataaaca gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt
     5641 tacggtcata gatcacgtca aaataagaag agggaagaca acattagcaa ggttgtgtac
     5701 atttgtacac aattgcagac agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc
     5761 gagaaaaaat tatccaggcc acgctggagg cggtgaaact ttacggaata catgctgtta
     5821 cgcaccgcaa aattgctacc cttgccgggg taccgttggg atcgatgacc tactattttt
     5881 caggaattga tgagttgttg ctggaggcgt tcagcagttt tactgagatt atgtcccggc
     5941 aatatcaggc attttttagc gatgttagtg atgctcaagg cgcatgccag gctatcaccg
     6001 atatgatcta cagctcacag gttgcaacgc cggataatat ggagctgatg taccagctct
     6061 acgcgctggc tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg atgcagcgca
     6121 gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta
     6181 ttgaggggat gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc gaggagattt
     6241 tgaggatggt tgagagggtt gcagggtagt agataaattt tagataacaa aaaacccatc
     6301 aaccttgaac cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag
     6361 cagtggcact aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt
     6421 tcattttcag ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc
     6481 cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg
     6541 atgttactgc gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc
     6601 atcattgcgc cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa
     6661 cagataacca cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc
     6721 gcgcccaggc cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc
     6781 atcaagccga actctttcac catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg
     6841 tggttagcac gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc
     6901 atgccgaaac tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca
     6961 aagaaggcgc aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg
     7021 cgatcggcga tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg
     7081 ttaagcacga cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg
     7141 aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt
     7201 ttaacgacca cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct
     7261 ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct
     7321 atttcatcgc ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt
     7381 cgaatacgtt caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg
     7441 ccgtcggtcc aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa
     7501 accttacggt tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc
     7561 tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga
     7621 ccgattaaat aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc
     7681 ctgctttcca tgccggaatg atgcagtgta tcgccagcac cgaccagaac cggtgtcgac
     7741 gtcatagagc ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct
     7801 aaccctaagg cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt
     7861 ttcccatcac gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa
     7921 ataaacagca taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct
     7981 aataacagcg atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt
     8041 aactttccga gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca
     8101 tttaacaatt cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg
     8161 ttgaaagaaa tggtaattta cgataatgtg ttttaccaga attcagggcg cagattcatt
     8221 cagcgcacct aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta
     8281 tcaacggcta tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct
     8341 tactgaacgc cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt
     8401 gaaacataaa caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg
     8461 cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg
     8521 cttgctgttt ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt
     8581 cctgaaacct ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct
     8641 gtttttttca ccgacgcgct cattctggca agagctggca tggttactaa gcgcggtgtt
     8701 ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca
     8761 cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt
     8821 ttctttcacc catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat
     8881 ctgcggcacg gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc
     8941 ttccgcacga atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc
     9001 agccagatct tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg
     9061 cattatttct ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga
     9121 aagtaggttt aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt
     9181 gaaaagtttt agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa
     9241 ttgcctaaag gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg
     9301 ctgtcggtaa gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt
     9361 ctgatgattt ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag
     9421 gtggcaaaac gtcttgcccc acagctgatg aaccgtccac cgcaatattc acgttcagaa
     9481 agagaaaaag ataatgacgc caaccattga acttatttgt ggccatcgct ccattcgcca
     9541 tttcactgat gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc
     9601 gacgtccagt tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt
     9661 acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt
     9721 ctgggtgttc tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg
     9781 cctggcggaa caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt
     9841 aaccgcagcg gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat
     9901 tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg
     9961 ccttggctgg cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt
    10021 gcatgaaaac agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact
    10081 ggcggaatat tacctcaccc gtggcagcaa taatcgctgg gatacctgga gcgatcatat
    10141 ccgccgaaca atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg
    10201 ttgggcgacg cgctaaaacc gctacgtcga tgtatgatac gcgggctttt gaccaggtct
    10261 gacagagagg tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg
    10321 taagcggctg cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct
    10381 ttcttgctac atgaacataa atcctgcggc gtcttctatt cactacaaag gccgcaagtt
    10441 accccatttt gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc
    10501 ggcactgcgc cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc
    10561 ccgggcgcgt gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc
    10621 tgtcacgggc attgcgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg
    10681 tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga
    10741 gactcgtcag gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct
    10801 ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggtga
    10861 tgaagtggtc gctgcgattg aacggcgggc gaaagagggc tattttcgtt ccaatttgca
    10921 tcgtggcggc gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa
    10981 agccgcgcga acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg
    11041 gccgttggtg atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg
    11101 tatcgatatc gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga cagaatattg
    11161 cctgaaaacg ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt
    11221 gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt
    11281 cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga
    11341 atgtgataac tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga
    11401 tgccaaaatc gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc
    11461 ttcagccgta ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt
    11521 gaaagcattt cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt
    11581 atccgttatg cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga
    11641 gcagatttcg atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga
    11701 tgaagggcaa aacaacgtta ccgaaaacta ttttctccac tgataattcc tttcgagcac
    11761 gcagtcgctg ctgcagtgac tgcgcgctgc aaaattatct gctgttttta accttttctt
    11821 aaagattatt tcacttctct tgtgtcgatt tggctttatc acatagagca aatacgcata
    11881 aaaatttgtt aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta
    11941 ttcttgcgat gcttctatat agcgagcagt gctggccggg agagagttct cttttcttac
    12001 accgcgccga taaaaaaata tgcacgttta ttgcatatct ttcagtgtga caacttttgt
    12061 tcgtttgtta acgaactttc agaaggaaag agatgtgacc gccttaaata aaaaatggct
    12121 atcgggtctg gttgcgggtg ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca
    12181 aaaaacactc cacatttata actggtctga ttatatcgcc cctgacacgg tggccaattt
    12241 tgaaaaagaa accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga
    12301 aggcaaatta atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct
    12361 agaacgccag ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg
    12421 gaagaatctc gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt
    12481 tgctatgccc tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc
    12541 tgtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct
    12601 ggaaaaactg aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac
    12661 cgtgttgaat tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc
    12721 ggcaaccgat ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata
    12781 cattaacgac ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg
    12841 gcaggcgtca aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc
    12901 aaaagaaggg gcgatggcgt tctttgatgt attcgccatg cctgcggatg ccaaaaacaa
    12961 agacgaagcc tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc
    13021 cgaccatgtg ttctatgtca acgccaataa agcagccacg ccgctggtga gtgcggaagt
    13081 ccgtgagaac ccgggtattt atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa
    13141 agtgcaggac ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg
    13201 aaaataatcc gcagccttag atgccgggcg ggcgcaccac acccgccggc aattcgcacc
    13261 atcatggtgc gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc
    13321 gcccgcaggc gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca
    13381 aatcctacga tggtcaacat gcggtggatg atgtcagcct gaccatctac aaaggtgaaa
    13441 tcttcgcgct gctgggcgca tccggctgtg gcaagtccac gctgctgcgt atgctggcag
    13501 gtttcgaaca accttctgcc ggacagataa tgcttgatgg cgtcgatttg tcacaggttc
    13561 cgccttacct gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga
    13621 ccgtggaaca gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattt
    13681 ccagtcgggt caatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgtaaac
    13741 cgcatcagct ttctggtggt cagcgacagc gtgtggccct ggcccgaagc cttgccaagc
    13801 gcccgaaact attactgctc gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc
    13861 ggatgcagct tgaagtggtg gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca
    13921 cccacgatca ggaagaggcg atgaccatgg cggggcgcat cgccattatg aatcgtggga
    13981 aatttgtcca gattggcgaa ccggaagaga tctacgagca tccgactacc cgctatagcg
    14041 ccgaatttat tggctcggta aacgtctttg aaggcgtact caaagagcgt caggaagatg
    14101 gcctggtgct tgattcgccg gggctggtgc atccactgaa agtcgacgcg gatgcctcgg
    14161 tggtcgataa cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag
    14221 agccgcccgc caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg
    14281 gcgatctttc ggtgtatcac gttcgtctga aaagtgggca gatgattagt gcccagctac
    14341 aaaacgccca tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct
    14401 gggaagtgga cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat acacttgaac
    14461 ctgctgccca gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga
    14521 agcatgggcg caaactggtc attgcgttgc catatatctg gttgatcttg ctgtttctgc
    14581 tgccatttct gattgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt
    14641 ataccgaact gatggagtgg gctgacgggc aactgtccat cactcttaat ctcggtaatt
    14701 ttctgcaact gaccgacgat tcgctctatt tcgatgctta tctccagtcg ctacaggtgg
    14761 ctgcgatttc gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc
    14821 acagtaagcc ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct
    14881 cgtttctgat ccgcgtttat gcttggatgg gaatattaaa aaacaacggt gtgctgaata
    14941 attttctgct gtggctgggg gttatcgatc aaccgctgac cattcttcat accaatctgg
    15001 cagtttatat tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttataccg
    15061 cgttgattcg tattgattat tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc
    15121 tgaaaacgtt ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga
    15181 tgctggtgtt tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg
    15241 acagcatcat gatcgggcgc gtgctatggc aagagttttt caataaccgc gactggccgg
    15301 tggcctcggc ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc
    15361 acaaacacca gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc
    15421 gccctggcgg attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat
    15481 gctggttatc tattcgttta acagctcgaa gctggtgacg gtgtgggccg gctggtcaac
    15541 gcgctggtat ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gttcaagcct
    15601 gacaattgct gcctgtgcgg caacggcggc ggcgatcctc gggactattg cggcggtggt
    15661 gctggtgcgc tttggcaggt ttcgcggatc aaatggcttt gcctttatga tcaccgcgcc
    15721 gctggtaatg ccagatgtca tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc
    15781 tcatgccatt ggctggcctg cggaccgcgg tatgcttacc atctggctgg cgcatgtcac
    15841 tttctgtacg gcttatgtgg cggtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc
    15901 gatagaagaa gccgcgatgg atctcggtgc gacgccgctg aaagtgtttt tcgtcattac
    15961 gctaccgatg atcatgcccg cgatcatttc tggctggtta ctggctttta ctttgtcgct
    16021 tgatgatctg gtgatcgcca gctttgtttc cgggccggga gccaccacgt taccgatgct
    16081 ggtcttttcc agcgtgcgga tgggggtgaa tccggaaatc aacgccctgg caacattgat
    16141 cctcggcgcg gtcggaattg tcggatttat cgcctggtat ctgatggcgc gcgcagaaaa
    16201 acagcggata cgcgacatcc aacgtgcaag acgtggctga agacactaaa atttgccaac
    16261 ctggctacat aatgccgcgc atgtcgcggc attgttttca tggaagacga aacgttggga
    16321 ttttttaaga aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg
    16381 gcggcgctcg ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg
    16441 ggcgtgcgcg gtattggcga gttcattcat cgcagcgtac aaacctggag tttaacgctg
    16501 gtctttttaa gcagtctggt gctggttttc attgagatct ggtgtgcgtt ttcactggtg
    16561 aaagggcgtc gctgggcgcg ctggctatat ctgctgacac aaatcaccgc cgcaagttac
    16621 ttgtgggcgg cttcgctggg gtacggttat ccggagctgt tcagcattcc cggtgaatca
    16681 aaacgtgaaa tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg
    16741 ctgctgttcg ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg
    16801 tcgcccctga tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg
    16861 tcgttcctgt cagtggataa cgcagccgat tccagagcaa ctctccgcta aaaccgccga
    16921 tcttaaaaat ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc
    16981 ggaacaaggg tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaacact
    17041 gctcggtatg ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc cgctttatcc
    17101 agcctcattt gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgag cggggttaac
    17161 gccctacaac gtggcgcgta agcgtggcga actgaaatac attctgctga ctgaaagcca
    17221 gagtgatggc ggcatgatgc tgcgctttgt actgcgttct gataccaagc tggcgcaact
    17281 gcgtaaggcg ctgccgtggt tacacgaaca actaccgcag ctgaaagtta ttaccgtcaa
    17341 tattcagccg gtacatatgg cgattatgga aggggagacg gagatctacc tgaccgaaca
    17401 acaggcactg gcggagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt
    17461 ccagactaat ccggcggtcg ccagccagtt gtacgccacc gcgcgcgact gggtacgaca
    17521 gctgccggtt aaacatatgt gggatctgtt ctgcggtgtg gggggctttg gtttacactg
    17581 cgcgacgcct gacatgcagt taaccgggat cgaaattgcg ccagaggcca ttgcctgtgc
    17641 aaagcagtca gccgctgaac tgggcttaac tcgtttacaa tttcaggcgc tggactccac
    17701 tcagtttgcc actgctcagg gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg
    17761 cggcattggt aaaccgctgt gtgattatct ttcaacgatg gcaccgcgtt ttattatcta
    17821 ctccagctgt aacgcccaaa ccatggcgaa agatatccgc gaactgccag gttaccgcat
    17881 cgaacgggta cagctttttg atatgttccc gcacaccgcg cactatgaag tgttgacgct
    17941 gctggtgaag caataaaaaa gccacaggtg cggcttcaga ttgctgatca ggtgcgcttt
    18001 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaatcatgc taaatcaata
    18061 cattgcagag ttgatgtggg ccggataagc gcagcgcatc aggcaatctt acttttgtca
    18121 tcagtctcaa ttactgcgga aaccactggt cattgatttt ctgatatgta ccgtcagctt
    18181 taattgctgc cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag
    18241 cgatacccag gccggtgccg aagtattgcg gatcggtcac tttttcagtc gcaacgccca
    18301 gttgcggatt ggttttcagc cattcgttta ccaccgcggt gtcaccaaat accccatcaa
    18361 tacgaccatt tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca
    18421 cttccgggtg ctgatcctga atatatttct ggtgcgtcgt accgttttcc atcccaatgc
    18481 gtttgccttt caggtcggca aacgttttgt aggtatcttt tttggaaatc acgacggctg
    18541 agttttcata gtagggtgtg gtaaacgaaa cctgcttgct acgctccggg gtgatatcca
    18601 taccggagat tacggcgtca tattttctga atttcagtga cgggatcagg ctgtcgaacg
    18661 cgtgattagt aaaagtacat tctgcctgca tttgtttgca caaggctttt gccagatcga
    18721 tatcaaagcc gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg
    18781 aaacgccaaa attgattttc tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag
    18841 cggcaagaac taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc
    18901 gtgtctgcgg catgggataa caatgccatc aagtgaattt atatgcaata aacatgatta
    18961 aataatttaa atgaaataaa aaaagacgga taacttagtg agttgtccgt cttcattata
    19021 agaatttatg cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg
    19081 tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat
    19141 gtacgcagct acattaattc cgccgttcga atgccagtgc tttgcgctcg atcagacgca
    19201 tcatcagcgt cagcaggccg ttgacgacca ggtaaataat ccctgccgca ccgaacacca
    19261 ttacatcgta ggtgcgtccg tacaacaact ggctgtatcc catcacttcc atcagcgtaa
    19321 tggtgtaagc cagagaggta cttttgaaca ccagcaccac ttcgttggaa taagaagaga
    19381 gcgagcgttt aaaggcatac ggcagcagga tcgccagcgt atcttttttg ctcattccca
    19441 gggcgctaca ggactgccac tgaccttctg ggatcgcacg aattgcaccg taaaacagct
    19501 gcgtggtata cgccgcactg ttcagcgata acgcaatcag cgcacataac cacggttctg
    19561 acaacaaatg ccacagtgcc ggatactcct gcaacgtcgg aaattggccc ggcccgtaat
    19621 aaatcaggaa gatctgcacc agcagcggcg taccggtaaa cagcgtgata taaccccgca
    19681 ccagccacac cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa
    19741 tcagtgccac aatcagcgag gcaacggtta gcgtcaggct ggtgtgcagc cctttcatca
    19801 gctcgggtaa atactcaaac attagctggg cctccgctca aaacgtgtcg cgcgcaggtc
    19861 aatgcgtttg agaatgtact gactgagcag ggtgatcacc aggtaaatcg ccgccgccac
    19921 aatgtaccag gtaaatggtt cctgggtacg agtagcaatg cttttggttt gtaacattaa
    19981 atcattcaca ctaatcaaac tgaccagcgc ggtatctttc agcagcacca gccactggtt
    20041 accgaggcca ggcagcgcat gacgccacat ctgcggcatc accagacgga aaaagatagc
    20101 cgatttcgac agccccagcg cctgaccaga ttcccactgg cccaccggta cagctttcaa
    20161 cgcgccgcgc agcgtttgcg aggcataggc ggcatacagc agtgacagag cgatgacacc
    20221 acaaaggaac ggactcacgt cgaagttctc aatgtccatc tgcaccggga tctgcacgaa
    20281 cccaagattg atagtgaagc catccgaaag cgtcagcagt aactgcgagg agccaaaata
    20341 gataaacagc actaccagaa tttctggcag gccacgcaga atggttacca gcgctgaacc
    20401 tgcccacgcg acaggacgcc atttcgctga ctcccatacc gcaaagaaca tcgccagcgc
    20461 cagcccgaca atcaatgcac aaacggcaag gccgacggtc atcccggcgg cgcttgctaa
    20521 aggaaaaaat tcattcatca ggaattactt ctggaaccat ttgttgtaga tggtttcgta
    20581 agtgccatct ttcttcactt tttccagcgc agtgttgagt ttctgctgca gttcagtgtt
    20641 gccctgacgt accgcgatgc cgaggccagt accgaaataa tctttatcgg tcactttatc
    20701 gcccactgcc gccagttttg ggttatcttt cagccactca gtgaccactg cggtgtcacc
    20761 aaatacgctg tcgatacgcc cgttttgcag atccagtttt gcgttctggt agctgtcata
    20821 cggaacggtg gtgatttccg ggtgcttatc cataatgaac ttctggtgtg tcgtcccgtt
    20881 ctgaacgccg acttttttgc ctttcagctg atcaacactg gtgtatttgc cttgctgacc
    20941 cacaaacagg gcagagttgt catagtacgg ggtagtaaac agtacctgct tttcacgctc
    21001 cggagtgata tccatgcccg ccatcactgc ttctacgcga cggaatttca ggcttgggat
    21061 caggctgtca aacgcctggt tagagaaagt acaggttgca tcaatctctt tacacagtgc
    21121 ctgtgccagg tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg attcaaacgg
    21181 aggatagggg gcttcggtag caaaacgaat ggtttcggca gctgtggcgg aaagactaaa
    21241 acctgcaatt aacgcggcaa tcagaacttt tttcattgtt gttatcccga atcttagtga
    21301 gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt
    21361 tctacgatat gaccattttc catatacacc actcggctgg cggttttacg cgccacttca
    21421 acttcgtggg tgacgatcac ctgggtaata ttcgtttctg ccagctcacg aatgatgctg
    21481 acgatttgtg ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc
    21541 tgcggttcca tcatcaacgc acgggcaata gcaacacgct gctgctgacc accagaaaga
    21601 tgcagcgggt aacgatcgct ataaggtttg agacgcagac gttccagcag tttttctgca
    21661 cgggccagcg cctgatcttt actcaacccc agtacacggc agggcgcttc aatcaggttt
    21721 tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa acaccatgcc aacgttacga
    21781 cgcaaatcgc gaatcgcttt gtcagagggt gttttggtga aatcgaaatg gttgcctgca
    21841 atgttgagcg taccggagcg cggcatctca agcagattga gtacacgcag cagcgagctt
    21901 ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc cctgtgggca atccagcgtg
    21961 atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc
    22021 attgacactc gtatactggc agtctgatag ctattgaggt cgaagatagt acctttgaca
    22081 gaataattat gcaatattta tgctttaaaa gttaaaagca aagcgcatta ttcaataaac
    22141 atagcacaaa ataacggggg cggtggtcgg tgagcataaa tgtcggcatt cctcacgaaa
    22201 tgccggataa tttacggggt ttattggttg atcaaggcgt tagcgattct cgatggactg
    22261 acggagcgta cccgccgtgg catgaacgct accgcctaag tagcgcacat cgtcgatgac
    22321 ccaacactgg ccttcctgaa tcattaacac ttcatcctgc cagccctggt caccctgttt
    22381 gagatccacc cgcaatggaa tgttacgggc atcacgatta gggatagtcg atgcactggc
    22441 aacgtgggcg ctgtctggca aggtggttcg actggagaac ggatcgctgc ttaacaattc
    22501 acgatgacta ttatcgcggc tggcatcgct aagttgggtt gccagtttgt cactcaaata
    22561 ggggcgcagg gcggtaatgt cattgctgcg atgttgaatg cggtagtcgt agaattgttg
    22621 agccacactg tcaggccctc cctggacaca ggaaccggtg cgggggccgt tatctttata
    22681 agctggcgtg acggtggtac aggcactcag cagcagtgca catggaataa gtagagatag
    22741 tgaacggtag cgcataatca tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa
    22801 acgctatcag attatttaat acattgtttt taatacaatt ctgaaacaat ctgaacacct
    22861 gattgggttt gagtccattg ctggctaccc actcaaggtt gctcgtagct gacttactta
    22921 aattacccaa acaatagaca gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga
    22981 aatgatcatt taaggtaaat atgatagttc tgaaatggtg gtataaatcc tcctgtagag
    23041 gttaagagca tctgcaagaa gccagtttca catttataac gcgtgataaa tgaatgcggt
    23101 tattgtctaa tcattacaaa atttttcaca tgcgataaga gatcctgcga ttaagatgat
    23161 tgccaaaatt acgttgtatt tttgaaaatg agcgaaattt gatagtccca gctttgactc
    23221 tcgataatat tatttatgct acaattgtgg gtaaagaatt taagctaatg aatttgagtg
    23281 aagaataaat taaattgctt cagtatttcg ttaattaata taaatacaaa aggcgttgtg
    23341 cttttataat tataaagcgc gaggagttcg gtggattatc gtttttcttg ggtgattttt
    23401 aatatgataa acaatatatc tgatcaagca tcatcatttc caggcacaca actaaatcaa
    23461 agtgataatt tcctggattc actgagggaa tttttcgcga tattaaattc cagtcgtaag
    23521 ggtgagttgt ctacatggga taccatctac ttgcatctga tattagcaat aaatgctgac
    23581 agtgatctca taaaaaatga tgtgttgctc gcggaaaata ttccatcagc gaattaccaa
    23641 tttaatacat tttttagcaa tactttcgag attgatgtta aaaaatatct gggtaaatca
    23701 gaagataacg aagttgaaat aaaagcgggt aatgaaagaa tctctattgg aatacgtaat
    23761 gtatcgaatg gaaaacttga aagacaacag tttcttttcc cactggacta tgagaataaa
    23821 ttacaagaac aattagataa gtattttacg attgaatcgc acccattatt atacaggtat
    23881 accattggta gtaagattgc gaatgtgatt tttgaaaagt tatacagtcg tattgacttt
    23941 aacaaagaac aatatatatc ctttataaag gacgcattta ttcattttta tgattatagc
    24001 aggcgatatg ctattagtga gaatatcgat aaagacgctg taacaaacaa tatcgcattg
    24061 atgagcactt tttatgacag tgataatacg tcaggtgagg ttttaaataa tgattttaca
    24121 gaagaagagt catttgaaac tgcgttagat gtagagcatg caatagtgct tggatttgct
    24181 gatgataatt ttgaaacaaa acctgttcat tatcaggatt tattaacacg atttagtgcc
    24241 tttcaagata ccgtgtttaa tctgtttcct gaaatgcact cttcgcatta ccacgatatt
    24301 tgtagcgtaa gtgtggatat gacaaaggga acgcaatgca tgattcactt gatggtgaat
    24361 gaagaagttt tcatgtctct gcctgttcca gttgccacga tggtacgtga agatgcaagt
    24421 aatttagtca atcttaaaac actactcaat gatggttgtt tcattaaata tagtcatttt
    24481 aatgacgtgg cattaataaa gcaaaacata agtaatctct atttatcaca cactgttgtg
    24541 aatgaaagca tattgaaaaa atgttgtttt gaaaacggca gtttaggtga tgttaaaata
    24601 acaaatagca atgtaataaa cagtgcattc aaaaatattt cgtttagatc tgttaaaatt
    24661 aataacgtaa atactcattc tttaaaattt attaactgca attttttcaa tgttgatatg
    24721 ataagagtta atctcagcaa atgtcttttt catgaatgta gtatgcatgg tgtcaagata
    24781 aaaccctggt tgcctgtaaa atggacaaaa gaattaataa gtgattattt gtatggctgt
    24841 ttactatctc tgtactctat ctgtgcaagg gatatatata atatgaacgc cgggaataac
    24901 gtgaaggttg ctgcggatgc tttccttgaa attattttct ctcttaaaaa taaatattgt
    24961 attaaactat taagcgctca ggatcgggcg tttatatacg agtttgcaag aatgatcttc
    25021 gcttatatta atgacaaaag cattgagatc ttattacttt cttgttttgc ggcggctgac
    25081 caaaaagcaa tccagcggta tagacctcag tcacaagatg gggaagactt taggagtcat
    25141 ttacaatata agttgcctct ttccgctcat taatgttgtt ggtgcaacat aataagacag
    25201 gtaagcggta ccctataatg aaaccaatat cgggttatgc aacggataat tgtaaagact
    25261 taagtgggtg tttttgacac aatgagatga tttcctaaac tgatcaaaac cttcttccca
    25321 acatggcata tcagtccgat aatgctttag agatcgaagg cttaacaaac aaggagatcg
    25381 atcatgcaat tttctacaac cccaactctg gaaggcctga ccatcgttga atattgcggt
    25441 gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgccggtatc
    25501 cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag
    25561 atcgcctttg aggaattagg ctcccaggcg cgggcgctgg gggccgatgc cgtagttggt
    25621 attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc
    25681 ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt
    25741 tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata
    25801 cccgacacca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg
    25861 cagatgattt tgatagctcg ctggcgacac tgactgataa gcaggtcagc tcgcattatc
    25921 tgatccctgc ggtgccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg
    25981 aacaagaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg
    26041 acacctctat tggcattgag ctggaaaacc gtggctggca aaaatcatcc ggagtgaaat
    26101 attttgcccc gtttgaacag gcacagattc aggcgcttat tccgctggcg aaagatatta
    26161 ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc
    26221 gcaaagacga tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg
    26281 cctggccgga tgcgcagcgg gtgaactttt accttgccgg gcgcgcgccg cacacaccgg
    26341 tagataccgc gtcattgctg gagcttttgg cgcgctacgg ttatgacgta aaacctgata
    26401 tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt
    26461 tatataacgg cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat
    26521 acgggcagga ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga
    26581 tgccttcatc cagggtgatg accggctgat aacctaactc ttcctgcgca cgcgtaatat
    26641 ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc ggctcttttg
    26701 ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtaaggga
    26761 cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca
    26821 gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg
    26881 cttcctggct tgccagccac attgcgtgca cggcattttc atagtaggtc atatcgacca
    26941 gcgcactgcc gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac
    27001 ggggaataaa gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc
    27061 gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc
    27121 gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatattg cgatggtggt
    27181 gataatcgaa gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga
    27241 cagcccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg
    27301 tcccccaggg tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca
    27361 ctttagcttg tgatgaaacc agctcggtca gatccgtcgg aacaaactct gcgcccattt
    27421 tttccagcaa tttacccatt gcttcgttgc gaccgctcgc acgcacgctg atgcctttct
    27481 ggcataaaaa ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct
    27541 tcatatcaat ccactgttgt tgagaaaatg acgtgcgcat tcttccgtga tttcccccat
    27601 gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc
    27661 tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat
    27721 caataaccag taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat
    27781 agtacgatag tcacctttat cttccagggt aaaacacagt cgtcccagcc ccggcgcttt
    27841 catgccaaat aacaacgtaa gttgcttttt cggttcaacg acaatcactt tccagctatc
    27901 caccgcatcg ccggtctgta aatattcgcg ctccgggcgg cctttcgcca gattatgacc
    27961 gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc
    28021 tttaccgccg atttggttca ccaccagcca taaagccgca aggctggcgg acgttttaac
    28081 ggtaaacccc gcctgtttgg cgaaataacc gtactccggt cgccagcggg caaaggcctg
    28141 agcgtcgtag ccccagtcgc tggagttgac cagtttttct tcctctttca acgtgctacg
    28201 taccgcgtca tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc
    28261 tgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga
    28321 agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa
    28381 ccagcggcgc ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac
    28441 ctctggtccg gcggcttcga agatgcggtg ttcgttggct ggatggtcta acagcgccac
    28501 cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg
    28561 cggcgttaac actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc
    28621 tgcgccaacg ataattccgg cccgaagttc ggtcactggt acattcgctt cacgaagaat
    28681 gtccgccgta gcctgacgag ctcgcagatg atccgactgc tcatgtggcg gggcctgcaa
    28741 cgaactgaga aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag
    28801 agccagctgg cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa
    28861 atagacggta tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac
    28921 tttatggcaa ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc
    28981 cgctaggatc tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc
    29041 actggcaccg agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg
    29101 cgcgccagtg ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca
    29161 ggcggacaat cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg
    29221 cggcagcatt ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat
    29281 ccgcgcctgc ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca
    29341 accgtgcgac gttatttttc agggcataca tcccggcggc agccagaatc ccggactgac
    29401 gcatcccgcc accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac
    29461 cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat
    29521 cacaatattg cgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc
    29581 gcgcaccgtc aacatgcagc gccagattgc gctcgcgggt aaattcccat gcttctttca
    29641 ggtattcacg cggcagcact ttgccgttgt gggtgttttc cagactgagt aatttggtgc
    29701 gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc
    29761 cgtcggcagc cgcgtcgatg ggttgcggct gaatactgcc cagcaccgct gcgccaccgg
    29821 cttcaaacag atagttatgc gcggcctgac cgacaatata ctcttcgcca cgttcgcagt
    29881 gactgagcag agcgaccagg ttggcctgag tgccggtagg cagaaaaatg gcggcttctt
    29941 taccggaaag ctctgctgca tagtcctgca gagcattaac ggtagggtcg tctccgtaaa
    30001 cgtcgtcccc aaccggggcg gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa
    30061 cggtatcact gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt
    30121 tttaccttag ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa
    30181 ttgcgcgcaa catatacagg ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa
    30241 tggctaattc ttctttggcg accaccacat ccaccaacac cggaccgtcg atagagaagg
    30301 cgcgttgcag ggcttcatca acttcagagg ctttttctac acggataccc gtaatgccgc
    30361 acgcttcggc aatgcgggca aagttggtgt cgtgcagctc agtaccgtct gtcaggtaac
    30421 ctccggcctt catctccatc gccacaaagc ccagcacgct gttattaaag acgataattt
    30481 tcaccggcag tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc
    30541 catcgccgca catggcgacc acctgacgtt ccggctcggt tgcctgcgca cccaacgcct
    30601 gcggcatggc gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt
    30661 tcattttcag ataacgtgcc gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg
    30721 cgtcatcggc ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct
    30781 cgctcggttt agctaaatcg tccagccctt tgcgggcatc gcggtaatct tccagcgctt
    30841 tatccagaaa cttgcgatcg gttttttctt ccaccagtgg cagcaatgcg cgcagggttg
    30901 atttgatatc gccgaccagc gccatatcta ccttgctatg cgcgccgatg ctggctggat
    30961 taatatcaat ctgaataatt ttggcatcgg tcgggtagaa cgcacggtag gggaactgag
    31021 taccgagtag cactaacgtg tcggcgttca tcatggtatg gaaacctgac gagaatccga
    31081 ttaacccggt cattccaaca tcatacggat tatcgtattc gacatgctct ttaccgcgta
    31141 gggcatgaac aataggtgct ttaattttcc cggcaaactc aactaactct ttatgcgccc
    31201 ccgcgcagcc gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca
    31261 gtttgcgtaa ctcttcttct tccggcatca cgactggctg tggcgcatga taccagtgcg
    31321 tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc cggtaataca acaacggaaa
    31381 cgccacggtt aagcaccgct ttgcgcatag caattgccag cacttgtggg atctgctccg
    31441 ggctggaaac cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt
    31501 cctggaaata gccgctgcca atttcgctgg agggaatatg agcggcaatc gccagtaccg
    31561 gaacgttatt gcggtggcaa tcgaataggc cgttgatcaa gtgcaggttg cctgggccgc
    31621 acgatccggc acagaccgcc agttctcctg aaagttgtgc ttcagcgcca gcggcaaacg
    31681 ccgccacttc ttcatggcgg gtggacatcc actcgatggt gcccatgcga ttaagactgt
    31741 cgctaagacc gttcagagag tcgcccgtga ctccccagat gcgtttcacg cctgccgatt
    31801 cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg
    31861 tgataacggt aacaagttta gttcatctga cggaggggga agggatggga gagaaaggag
    31921 gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg ggcataaggc gattatgcga
    31981 gaaccaaatc cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg
    32041 cgtcggtcag cgtcattgta ctgctcaccg tatattcacc ggaaaccact tttgtcttac
    32101 agcagccgca aacacccgca cggcaggcag cgacaaccgg aacgttattg ctttccagcg
    32161 cctccagtag cgtggtgcca accggggcgt aaaattctcg tgccggttgc agtttggtga
    32221 atttcagacc gctggtcgcc gcttctgcta ctggggtgaa gaatttctct ttaaagaaac
    32281 gcgtcacgcc gagcgctttc acttcctgct ctacccaatc catatacgga gccgggccgc
    32341 aggtcatcac ggtacgtgaa gctaagtcag gtacatctgc cagcagttcg cgagtgagac
    32401 gaccagcgat aaagccttcg gtaacgttat tttctgccac cagcgttacc ggatagttac
    32461 gccactcatc ggcgaaaata acatcctgcg gcgtacgcac gttgtagatc acccgcacat
    32521 cggcctgtgg acggttcttc gcaagccagc gacgcatcga cataatcggc gtgacgccgc
    32581 agcctgccgc cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca
    32641 tcgcgtccga aagccagaga taatcaccgc gttttacatc gcgcgtcagc cactgggagc
    32701 cgacaccgtc atcaatccgc cgcacggtca gggtgatata ttcactcacg cctggcgtgg
    32761 aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg cacgctgacc agtgcatatt
    32821 gcccggcgcg atatgggtag tgatcgtggc aaatcaggga aatcgtccac acatccggcg
    32881 tttcttgcgt aatgtgatga acctgtatcc gccacgggca ttgattcgtt ggcatcgtca
    32941 tcgacaaact ccttacgcgt tcaacagttg cttcatgtct tcttcaacag tggtgataga
    33001 acgcaggccg aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc
    33061 agtcgggccg gtgacgatat ttttcacgcc cagagaaagc agcgtcagca gaatgacaat
    33121 cgctttctgt tcaaaccagg agagcaccag cgacagcggc agatcgttca caccacagcc
    33181 cagtttctct gccagagtga cagccagaat aattgctgag taagcatcgt tacattgacc
    33241 tgcatctacc agacgcggca gaccttcgat atcgccaaac tcaagtttgt taaagcgata
    33301 tttaccacag gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt
    33361 gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca agcaggaaga tatgacgcag
    33421 tttttcacgg ctaaccagat caatcagcgt atcggctgca ccaagcagcg tctggcgacc
    33481 aaagcccacg gtgatcaggt gaggaatttc gctgtacggg aagcccgcca tttgttgcgc
    33541 ctgggcgatg accgcagaga aatcttcgcc atccagatga cgcacgccag gccagccaac
    33601 gatgctgcgg gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt
    33661 cgaggtcatc acgatggggc cagggaaacg agcgaactcc acttgctgat tctgccagcc
    33721 gctgccgtag ttaccgacca gatgcttgaa tttacgcagc tccgggtagc catgcgcagg
    33781 cagcatttcg ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt
    33841 gtagagatct ttgagatcgt gaccggaaat cagaatgcat ttacccgccg tcgctttgac
    33901 gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct
    33961 catcactttg aagttcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc
    34021 ggcaggccag gtccccagcc acgccatgat tttatggtac tgggcataaa tatcgttgtc
    34081 gtattgaccg agaacatgcg cgtgttccat ataggccgcc gcacctttca ggccatacag
    34141 gcacagcaga cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt
    34201 aaattctgct gcctgacgtt gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc
    34261 catcgggtta tcgacgcgcg cgttggcatc tacagccagg cattgtgctt tcagcgcctc
    34321 gcgcaaggca atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt
    34381 ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc
    34441 gtattcacgc gcttttaccg cccaggcaga aagcccttgc agcgccgcga tgagtaaatc
    34501 ctgaaggtca gaagtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt
    34561 ttctgccgga gtacggatag tttgttcaca ttgcacacaa aacatgatca caccttttaa
    34621 agttatattt aatatacatg tttaagggta agacgcttaa cgcggggata aaagggattt
    34681 ttcatgcaac tttaagggag attgatttag cgcaattttg gcggcaggga actaccgcca
    34741 gagaggtatt acgcagagaa aaaggcgatg aggatcggca ctaacaggct aagaataaaa
    34801 ccgtgaacaa ttgccgccgg gaccatatcc agcccgccag tacgttgaag aacgggcagg
    34861 gtgaaatcca ttgatgtggc accgcataag cccagtgcag tagagcggct gcggcgaatc
    34921 agcccaggga tcaacataat agcaatcagt tcacgggcca gatcattaaa aaacgccgcg
    34981 ctcccgatta ccggaccaaa agattcggtc aataaaatac cggaaagaga ataccagccg
    35041 aaaccggagg ccattgccag cgcggtattg atggggagat caagaataaa ggcgttaatt
    35101 aaaccaccaa ttaatgaact gacaaccacc accacggcga caatcattcc ccggcgatta
    35161 aggacaatct gctttaaggt catgccatta ttgcgcaact gaataccaac gaggaaaagt
    35221 agcaaaatta acgtgtattc actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc
    35281 agaccaatgg caaaaccaat cactactacg ccgcacagtt ttagcgactc cagcgccatc
    35341 gcaatacgcg acgggagttt ttcttgctga tggtggttgc gccacggcag gcctcgctcc
    35401 agccacatca gggcggcaat attacacagt aaaataacgg taatactgac ggcagaataa
    35461 tgcagaatcg ccaacaggtt actggcgagg ttatcgagaa acgccagact gatacccata
    35521 aaaaagagaa taaggtaaac catccagctt aatagctgat taatagcttt taacgcagct
    35581 tgttggcgaa gcggaatgag gtaacccaca atcaggggaa ccagaatgat taacagccca
    35641 gaaaacatga aaatccagtc cttgcaaaga agaagtcgaa atgcgcgatg acacactact
    35701 gaaagcggaa gggcgagtaa agttgcaatt aaaaggaaat gttatgcatc agagggggat
    35761 ttttaatcct atctgagtgt ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa
    35821 agccactggc ggagggagcg cgcagggggc agccagtcgc cgccgccccc tgcacccccg
    35881 ggctctggcg aacaaaatcg ccgctgcgcg gtaccttcag cttatcccct tcggcaaccg
    35941 ggtcgggcgc gaggtaacgt cctgtaagac gcgccctcag cccacatcca tgtgggctgc
    36001 cccggccttc ggggaacgct tcagcgattt tgacgccacc aatacccgtg cggtttgtaa
    36061 tgtcgaagat aaaacaacca gggcagataa aatgccaatg ctaattaatc acgcttttcc
    36121 agcagagtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
    36181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
    36241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
    36301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcacc gtggatcact
    36361 aacaggaagc ccgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
    36421 cctggtgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc
    36481 gttttaccac tggcaattaa atacagcagc gccgctgcaa caataccgcc gacaacctgg
    36541 gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc cgccagccca taaaccaata
    36601 gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcgaaggc catcgtcaga
    36661 acggtcaaac caaatgccag cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct
    36721 gcggccagta cagcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca
    36781 gctgctaatt ttctgaacat atccacctca attaaaaatt gaccctgtga aaa
//
LOCUS       DAJTNV010000032        36636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNV010000032 DAJTNV010000000
VERSION     DAJTNV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(152..1387)
                     /locus_tag="OO199_001660"
     CDS             complement(152..1387)
                     /locus_tag="OO199_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5535738.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1534..1610)
                     /locus_tag="OO199_001661"
     tRNA            complement(1534..1610)
                     /locus_tag="OO199_001661"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1574..1576),aa:Pro,seq:tgg)
     gene            complement(1653..1739)
                     /locus_tag="OO199_001662"
     tRNA            complement(1653..1739)
                     /locus_tag="OO199_001662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1703..1705),aa:Leu,seq:cag)
     gene            complement(1760..1835)
                     /locus_tag="OO199_001663"
     tRNA            complement(1760..1835)
                     /locus_tag="OO199_001663"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:His,seq:gtg)
     gene            complement(1894..1970)
                     /locus_tag="OO199_001664"
     tRNA            complement(1894..1970)
                     /locus_tag="OO199_001664"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1934..1936),aa:Arg,seq:ccg)
     gene            complement(2073..3458)
                     /locus_tag="OO199_001665"
     CDS             complement(2073..3458)
                     /locus_tag="OO199_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5535739.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3649..4389)
                     /gene="rffM"
                     /locus_tag="OO199_001666"
     CDS             complement(3649..4389)
                     /gene="rffM"
                     /locus_tag="OO199_001666"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5535740.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4392..5744)
                     /gene="wzyE"
                     /locus_tag="OO199_001667"
     CDS             complement(4392..5744)
                     /gene="wzyE"
                     /locus_tag="OO199_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5535741.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5741..6820)
                     /gene="rffT"
                     /locus_tag="OO199_001668"
                     /gene_synonym="wecF"
     CDS             complement(5741..6820)
                     /gene="rffT"
                     /locus_tag="OO199_001668"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5535742.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6817..8067)
                     /gene="wzxE"
                     /locus_tag="OO199_001669"
     CDS             complement(6817..8067)
                     /gene="wzxE"
                     /locus_tag="OO199_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5535743.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8069..9199)
                     /gene="rffA"
                     /locus_tag="OO199_001670"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8069..9199)
                     /gene="rffA"
                     /locus_tag="OO199_001670"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5535744.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9204..9878)
                     /gene="rffC"
                     /locus_tag="OO199_001671"
                     /gene_synonym="wecD"
     CDS             complement(9204..9878)
                     /gene="rffC"
                     /locus_tag="OO199_001671"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5535745.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9856..10737)
                     /gene="rfbA"
                     /locus_tag="OO199_001672"
     CDS             complement(9856..10737)
                     /gene="rfbA"
                     /locus_tag="OO199_001672"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5535746.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10756..11823)
                     /gene="rffG"
                     /locus_tag="OO199_001673"
     CDS             complement(10756..11823)
                     /gene="rffG"
                     /locus_tag="OO199_001673"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5535747.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11823..13085)
                     /gene="wecC"
                     /locus_tag="OO199_001674"
     CDS             complement(11823..13085)
                     /gene="wecC"
                     /locus_tag="OO199_001674"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5535748.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13082..14212)
                     /gene="wecB"
                     /locus_tag="OO199_001675"
     CDS             complement(13082..14212)
                     /gene="wecB"
                     /locus_tag="OO199_001675"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5535749.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14268..15314)
                     /gene="wzzE"
                     /locus_tag="OO199_001676"
     CDS             complement(14268..15314)
                     /gene="wzzE"
                     /locus_tag="OO199_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5535750.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15326..16429)
                     /gene="wecA"
                     /locus_tag="OO199_001677"
     CDS             complement(15326..16429)
                     /gene="wecA"
                     /locus_tag="OO199_001677"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5535751.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16669..17928)
                     /gene="rho"
                     /locus_tag="OO199_001678"
     CDS             complement(16669..17928)
                     /gene="rho"
                     /locus_tag="OO199_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5535752.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18013..18114)
                     /gene="rhoL"
                     /locus_tag="OO199_001679"
     CDS             complement(18013..18114)
                     /gene="rhoL"
                     /locus_tag="OO199_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5535753.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18255..18584)
                     /gene="trxA"
                     /locus_tag="OO199_001680"
     CDS             complement(18255..18584)
                     /gene="trxA"
                     /locus_tag="OO199_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5535754.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18715..19980
                     /gene="rhlB"
                     /locus_tag="OO199_001681"
     CDS             18715..19980
                     /gene="rhlB"
                     /locus_tag="OO199_001681"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5535755.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20114..21598
                     /gene="gppA"
                     /locus_tag="OO199_001682"
     CDS             20114..21598
                     /gene="gppA"
                     /locus_tag="OO199_001682"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5535756.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21645..23666)
                     /gene="rep"
                     /locus_tag="OO199_001683"
     CDS             complement(21645..23666)
                     /gene="rep"
                     /locus_tag="OO199_001683"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5535757.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23753..24034
                     /gene="ppiC"
                     /locus_tag="OO199_001684"
     CDS             23753..24034
                     /gene="ppiC"
                     /locus_tag="OO199_001684"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5535758.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24080..25555)
                     /gene="ilvC"
                     /locus_tag="OO199_001685"
     CDS             complement(24080..25555)
                     /gene="ilvC"
                     /locus_tag="OO199_001685"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5535759.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25705..26595
                     /gene="ilvY"
                     /locus_tag="OO199_001686"
     CDS             25705..26595
                     /gene="ilvY"
                     /locus_tag="OO199_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5535760.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26592..28136)
                     /gene="ilvA"
                     /locus_tag="OO199_001687"
     CDS             complement(26592..28136)
                     /gene="ilvA"
                     /locus_tag="OO199_001687"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5535761.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28139..29989)
                     /gene="ilvD"
                     /locus_tag="OO199_001688"
     CDS             complement(28139..29989)
                     /gene="ilvD"
                     /locus_tag="OO199_001688"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5535762.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30054..30983)
                     /gene="ilvE"
                     /locus_tag="OO199_001689"
     CDS             complement(30054..30983)
                     /gene="ilvE"
                     /locus_tag="OO199_001689"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5535763.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31003..31266)
                     /gene="ilvM"
                     /locus_tag="OO199_001690"
     CDS             complement(31003..31266)
                     /gene="ilvM"
                     /locus_tag="OO199_001690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5535764.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31263..32909)
                     /locus_tag="OO199_001691"
     CDS             complement(31263..32909)
                     /locus_tag="OO199_001691"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5535765.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32912..32962)
                     /locus_tag="OO199_001692"
     CDS             complement(32912..32962)
                     /locus_tag="OO199_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535766.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33048..33146)
                     /gene="ilvL"
                     /locus_tag="OO199_001693"
     CDS             complement(33048..33146)
                     /gene="ilvL"
                     /locus_tag="OO199_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5535767.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33498..35018
                     /locus_tag="OO199_001694"
     CDS             33498..35018
                     /locus_tag="OO199_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5535768.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35043..35381)
                     /gene="maoP"
                     /locus_tag="OO199_001695"
     CDS             complement(35043..35381)
                     /gene="maoP"
                     /locus_tag="OO199_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5535769.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35500..36339
                     /gene="hdfR"
                     /locus_tag="OO199_001696"
     CDS             35500..36339
                     /gene="hdfR"
                     /locus_tag="OO199_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5535770.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36435..36510)
                     /locus_tag="OO199_001697"
     tRNA            complement(36435..36510)
                     /locus_tag="OO199_001697"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36475..36477),aa:Trp,seq:cca)
     gene            complement(36519..36595)
                     /locus_tag="OO199_001698"
     tRNA            complement(36519..36595)
                     /locus_tag="OO199_001698"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36559..36561),aa:Asp,seq:gtc)
BASE COUNT         8944 a        10033 c         9229 g         8430 t
ORIGIN      
        1 ccgcacgtaa aggctttgcc ggatacgatc acccaaacca gtatccacag aacgctgcaa
       61 tttattgaat ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc
      121 aaccgcaggc ggcttccaaa tggccgcttt tttacttatt caccaccatc aaatgtgcct
      181 gcataatgtc actggccggg cgaccgtgcg ccagcaaatc agccattgct ttaagatatg
      241 gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc cccggtttgc
      301 cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc ccaacatgct tttaacacgt
      361 tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac acctgctgtt
      421 gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc cgatattgcg
      481 gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc
      541 cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc
      601 attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg tcaaaaatcg
      661 ccaccagaaa ttcaccaaat tcatcggggc gcacagacca ttccgtcagt tccccctgaa
      721 tatcaccagg cgcatgtagt ttcagtccat cgcggacagc tgtttcatca gccaggcgct
      781 cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa tcataaacct
      841 gtaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag tcgacatgat
      901 gtttttgcag gagcgtcagg gcacgcatca ccagtttatg cgtgggtctg ccgcctttgg
      961 tcacgcgata ttgattgtgg atctcagccg gaccatccag cgataaccca acaagaaaat
     1021 tattttctgc cagaaatgca caccattcgt catcgagcag cacgccgtta gtctggaaac
     1081 tgttacttat cttcctgcca gcaccatatt tcgcctgtag cgccacggca cggcggtaaa
     1141 actccagccc gagtagcgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgca
     1201 gttcgctggc ggcgatatag tgacggatat acgcttccag cgtgtcatcg tccatatgcg
     1261 tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca
     1321 gattacaatc ggaaccgctc ggtttcgcta tcacatgaaa agcacgcgtt ggaacctgtt
     1381 gcagcatggt cgctccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc
     1441 gtggggcaat gagttattac gggcgtttat tgcgaaggga gggtgacgaa atgcacagaa
     1501 aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc
     1561 cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct
     1621 ttttgaattt ttagttcaat tctttaaagt cgtggtgcga ggggggggac ttgaaccccc
     1681 acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca
     1741 ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc
     1801 acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat
     1861 tactaccacc gcagctcaag cgccggtact aaatggcgcg cccgacagga ttcgaacctg
     1921 agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt
     1981 tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg
     2041 ctcgtttggt tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttaccg
     2101 tggcgattaa gcccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgccg
     2161 acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg
     2221 aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca
     2281 atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc
     2341 accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga
     2401 atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca
     2461 accggtacgc cgtgacggga aacttttgcc attgccgccg gtaactgacg gtttttcgcc
     2521 agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc
     2581 gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc
     2641 agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg
     2701 aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac ggcactgcgc
     2761 agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg
     2821 taggacgcca ccacaataca cagagcggtc aggaaccctt tccagccacc cgcaaagaaa
     2881 ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag
     2941 aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg
     3001 aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc
     3061 gccaccgcga tcaatgcggg tatccactgc gccatctccg gggaccaaaa ctggacataa
     3121 acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca gtaagaccag
     3181 gcggtgagat agccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg
     3241 gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa aacgaacagc
     3301 ccggcgatga tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc
     3361 cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct
     3421 tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca
     3481 tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta
     3541 attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc
     3601 agacaggcga cgaagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc
     3661 ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg
     3721 cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt
     3781 tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg
     3841 caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac
     3901 gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa
     3961 ataaccatcc tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt
     4021 ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc
     4081 gcgcgccatc agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg
     4141 gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc
     4201 gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt cggcattaat
     4261 ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga gggcatgctg
     4321 catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt
     4381 gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg
     4441 agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag
     4501 accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg tgccagcacg
     4561 atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta
     4621 gtctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag tccaaccaca
     4681 atcgccccga gtgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta
     4741 ggcgagatcg ccagtccgga gtggttattc agtacttccc aggtaaagta attggctgag
     4801 ttcagcacca tactcgggcg ccccggccac agccaggaag ggataaagac atagaaatcg
     4861 cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc
     4921 agattctccc acggtgaaaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca
     4981 tcgccgctca cattcattcc atagcgtttt aacgccagcc agaacatgcc aacaatcccc
     5041 agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac
     5101 aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc
     5161 agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc
     5221 agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg
     5281 ccggagactt cactggaaaa gatctgactg taggagttaa gccggaacag caaaaagccg
     5341 ttgtgcatga agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta
     5401 agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt
     5461 ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc
     5521 aacacctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg
     5581 ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg
     5641 aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag
     5701 agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg
     5761 caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt
     5821 ttttatccgc cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt
     5881 cggtagtaaa cagcactggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc
     5941 tgttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct
     6001 ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa
     6061 attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct
     6121 gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt
     6181 ttaccgtatc gccaaattgc tgatgaacgg cacgtaaagc ggcgacatgc tcattgctgc
     6241 ggtcgccgga gttccccacc agaatggtca tttttccttc acgttgccga tcgttcgcca
     6301 tcatattgag agaagggtcc atccgcgtcg ggaagtacag cagttctccc cgtacctttg
     6361 ggtgcgtttt ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag ccgactcgct
     6421 tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg gaaagctcat
     6481 acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca
     6541 gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag
     6601 cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag
     6661 aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg
     6721 cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt
     6781 gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa
     6841 aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc
     6901 gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg
     6961 gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa
     7021 gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat
     7081 agcggtaaat ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt
     7141 aaaactcgct gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt
     7201 gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc
     7261 cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc
     7321 atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt
     7381 caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc
     7441 ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttaatcaaca tgatggcggc
     7501 aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc
     7561 caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa
     7621 cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc
     7681 gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa
     7741 caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa
     7801 accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg
     7861 gtactgggca acgtatttgg ttacaccgtt aaagatgcca gccccggcaa gcacgccgag
     7921 cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac
     7981 cgccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt
     8041 ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta
     8101 ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc
     8161 tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagtgttcc cccgcagggc
     8221 aaccgtgcag cggaatgtaa tgaaacaccg ccatgatttc cgcttctttc agaaagttaa
     8281 tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca
     8341 cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg
     8401 tatcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca
     8461 gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc
     8521 gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac
     8581 ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac
     8641 cacccgccgt gtagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc
     8701 ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttcc accacaaaca
     8761 aattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat
     8821 ggaccggcac gataacgcgc gttttgtcgg tgatcgctgc ttcaatcaac gtttcgtcga
     8881 tgttcatggt gtccgggcga acatccacaa agacgatttt cgcgccacgc agcacaaagg
     8941 cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat
     9001 cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg
     9061 cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac
     9121 acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg
     9181 gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc
     9241 gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc
     9301 aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct
     9361 gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag
     9421 acatagccgc gaatatcgcc ggacgccgca cgtagaatca gacattgatg atcaaaggtg
     9481 ccgcgcacgg cattttcaat ccactgtgca taaaagcgac cgctggcgtc aggcgcatac
     9541 cacggcgcac gaaaacggct ttgcgcaaat gcggcgctag ctaactgacg taatgcggga
     9601 atatcggtct cttgtgccac gacagcaccg ctatcactga cattgttcac gggtagcgcc
     9661 aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc cagttccccc
     9721 gtatttgatg ccgcaatttt ggcctgcacc cgtgaccatg gcgctaaggc gtctgacgtc
     9781 aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt
     9841 tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg
     9901 gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca
     9961 gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac
    10021 tgtctgcaca aaagtgctgg cctcaatcag gctgtcgtga gtgccggtat ccagccaggc
    10081 aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat
    10141 ggaggtaatc tccagttcac cgcgctccga cggcttcacc tgctttgcgt actccacgac
    10201 tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt
    10261 ttcttcaagc gagatagcgc ggaaattgtc gtcaaattcc accacgccaa agcgttccgg
    10321 gtccatcacc tgatagccaa aaaccgtcgc cccttccgtg cgcgccgcaa catgacgcag
    10381 cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc
    10441 accattaagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg
    10501 ttcggcatat tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata
    10561 acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca gcatcagcac
    10621 cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc
    10681 gcgcgtaatc ggatgcaatc gggtaccgga accgcccgcc aggataatac ccttcatgtt
    10741 tgcctccgct gaaaattagc ctttcagacc taaacgctca ccctgatagc tgccgtcctg
    10801 cacctgcttc caccagcttt cattagccag ataccactga accgttttac gcattccact
    10861 ttcaaaggtt tcctgcggca gccagccaag ttcacgggca attttcgaag catcaatggc
    10921 ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaggtcac gatagtgcgc
    10981 cacgccgtgc ggcttgttcg aagccagttc ttccagcagc tcgcaaatgg tttccacaac
    11041 atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt
    11101 ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg
    11161 ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg
    11221 gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgatcagcgt
    11281 cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc
    11341 ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg
    11401 caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc
    11461 ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat
    11521 gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc
    11581 cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc
    11641 gcagatatca actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat
    11701 caggtttccg gcgtaggtca gcttatcgac cactaccacc gcatcgctcg tttcgttgat
    11761 gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct
    11821 catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg
    11881 ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc
    11941 tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta
    12001 ggctcaacaa ccagcgtttc gccgctgtgc cactgggcga tcagttcagc gatttccatc
    12061 gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt
    12121 ttcagttcac tggcgcgttt atcggtagca gcaaggcagt cggcaaccgc cgctttcacc
    12181 tgatcgataa cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg gataagccgc
    12241 gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc
    12301 acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt
    12361 tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata
    12421 ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg
    12481 acgacacact caccttcgag gaaaattttg tacaattcgc tggcgcgggc cgaacaaacc
    12541 ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt
    12601 aatacacgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg
    12661 aaagtcagat ccgggcgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc
    12721 ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga
    12781 gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtaggtaca
    12841 gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct
    12901 acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg
    12961 atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca
    13021 aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa
    13081 ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat
    13141 caccatacgg gttatgggcg cggctcatag cttgatattc gttttcgtct tttaaaagac
    13201 gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg
    13261 tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag
    13321 gcgcttcttc ctgaatgccg cctgagtcag tcaaaatcag ccaggcgtgg ttcatcagcc
    13381 agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa
    13441 tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt
    13501 cctggtgcgt ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag ccacgaccga
    13561 aactctcacg cctatgaccg gtcaccagaa tcatcttttt atcgggggcg ataaacgggt
    13621 aatttgccgc cagttctgaa cgcagcgtgt cgctgctcat cacctggtca cgcacccata
    13681 acagtgcatc aatgactgta ttaccggtaa tgaagattcg gctatccgca acgttttcac
    13741 gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg
    13801 tcaatgtacg gttagcctct tccggccacg gcgaatagag agcgcccgtg cgcagaccag
    13861 cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg
    13921 tcgtcgtatc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta
    13981 gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga
    14041 gatcgtagtc aggtacaatg gaaaagagtt tcagtacctg atcaagcatc tcccgatgct
    14101 gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg
    14161 gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc
    14221 tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa
    14281 cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc
    14341 aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga
    14401 taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa catggctcga
    14461 ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc
    14521 tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca
    14581 tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatactg
    14641 ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc
    14701 atctggatgg tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca
    14761 cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg
    14821 gcaataagct tcacgctgtc attgaccgcg cgagtaaagt ctccggggat aaactggatg
    14881 ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt
    14941 ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata
    15001 acaaactctt tataggcttc gtccatgacc gatggttgat cggcagaagc catgtttgaa
    15061 cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc
    15121 gttggacgat cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc
    15181 agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag
    15241 gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc
    15301 attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc
    15361 tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc
    15421 aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa
    15481 aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca agggtaatca
    15541 gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac
    15601 ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat
    15661 ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc
    15721 cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat
    15781 cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca
    15841 ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca
    15901 aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc
    15961 catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac
    16021 caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa
    16081 gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta
    16141 cgctgatatc aaaacgatca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga
    16201 gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg
    16261 cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg
    16321 gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg
    16381 tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc
    16441 tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc
    16501 gttccgaacc caccagccct gtgcaaaaat caaccatttg atatgcaaat tgcataacat
    16561 gccagcaaat tccaggaatt ttcctaacat cgccagcgcg acattaagat tacagatata
    16621 aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt
    16681 catcatttcg aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc
    16741 cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag
    16801 ttcttcctga gtcgtgagca gctcttcttt acgggtaccg gaacggttgt agtcgatagc
    16861 cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc
    16921 tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc
    16981 gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg
    17041 acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac
    17101 agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt
    17161 gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga
    17221 tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt
    17281 tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta
    17341 agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg gcggtgccac
    17401 aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa
    17461 atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt
    17521 taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt
    17581 aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat
    17641 ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc
    17701 aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat
    17761 ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc
    17821 aaaaataatg tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag
    17881 agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt
    17941 tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat
    18001 gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga
    18061 actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga
    18121 taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa
    18181 gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg
    18241 aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac
    18301 agtgcgccca ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata
    18361 ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg
    18421 gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac
    18481 ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc
    18541 gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga
    18601 ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt
    18661 atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc
    18721 aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa
    18781 gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg
    18841 acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg
    18901 tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg
    18961 aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc
    19021 gacgcggaac cgctggcgga agctaccggc ctgaagctgg gcctggctta cggtggtgat
    19081 ggctatgaca aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg
    19141 ggtcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg
    19201 gtactggacg aagccgatcg catgtacgat ctaggcttta ttaaagatat ccgctggctg
    19261 ttccgccgta tgccgcctgc aaatcagcgc ctcaacatgc tgttctccgc cacgctttcg
    19321 taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa
    19381 ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa
    19441 aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc
    19501 gccaatacca aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt
    19561 gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt
    19621 acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt
    19681 ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac
    19741 cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa
    19801 gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc
    19861 aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg
    19921 cgcacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa
    19981 ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    20041 atgagcttac tgtagtaagt gattcgggtg aaagaatgca gccaacacag agacagattg
    20101 aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa
    20161 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    20221 taaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgacctct ctaatgaagc
    20281 aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc
    20341 ctcgcaaatt cgcgttgtcg ctacggcgac attacgactt gccgtcaatg cgggtgattt
    20401 tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga
    20461 agcgcgtctg atttatcagg gcgttgctca caccactggt ggtgccgatc agcgcctggt
    20521 ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    20581 gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa
    20641 tctggggcaa gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt
    20701 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    20761 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    20821 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    20881 gctgacgctg gaacgtgcat tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    20941 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    21001 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacac tgcgtaatat
    21061 tcaacgccgc tttatgatcg atattgatca ggcacagcgc gtagccaaag ttgcggctaa
    21121 cttcttcgat caggtggaaa acgaatggca tcttgaagca ataagccgcg atttgctcat
    21181 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac ttcaaacaag ctccgcaaca
    21241 cgctgcttat ctggtgcgta acctggatct tcccggtttt acccccgcac agaagaaact
    21301 gctggcgacg ctactgctca accagactaa cccggtcgat ctctcatcgc tgcatcagca
    21361 aaatgccgta ccaccgcgcg tcgcagaaca actctgccgt ttactccgcc tggctatcat
    21421 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    21481 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    21541 tattgctcag gaaaaccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    21601 agtaagtgcc ggatgcgatg ctgacgcatc ttctccggcc ttgattattt ccctcgtttt
    21661 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    21721 gcactgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    21781 aagcggctcg gctccgggcg caccagttcg ccgtactgac ggcgttcttt acacagcgta
    21841 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    21901 atattatctt catcgatact gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    21961 tagacataag gaaactccag cccttttgac gcgtggagag tcatcagttg cacctgatcc
    22021 agctcttctt cactctcacc acgctccatc atgtcgcgta aagtaaagcg cgtcaccacc
    22081 tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta
    22141 aacagttggt tgacgttctt catgcgcatt tcggcagctt tcgtgctggg cgatgtttcg
    22201 tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc
    22261 cgctccgcca gacgctggat ttctgccaac cagtgagtga agcgggtcaa tgcttcataa
    22321 ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    22381 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctcgcttc
    22441 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    22501 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg
    22561 tacgggatgc ggttttgcat caggaatttt tcaaacaccc gcgactgata gttaccgcga
    22621 taaagaatag cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca
    22681 ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt tagctctgtg
    22741 ccgtaaccca gttcggagaa cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg
    22801 atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc
    22861 ttcagcgccg gaaaatcctg gctcagcagc accaggtttt gcggacgtgc gccgcgccag
    22921 gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc
    22981 agtttcacca gttcatactg gctggtgttg gtgtcctgat attcgtccac cagcaggtag
    23041 cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagaag tagcgtcggc
    23101 aataaaatca gatcatcgaa gtcgagcaca ttacaggctt tcaggtgtgc atcatacagc
    23161 ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac
    23221 ggtgttttga gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact
    23281 ttgtcatctt caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg
    23341 tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc
    23401 agccccagcg tgtggaaggt tgagatcatc agcccatgcg cctctttgcg acccagcgtc
    23461 tgtccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata
    23521 tgtcgcgcct gataaccgca cccgcggatc agatgggcga ttttattggt gataacacga
    23581 gttttaccgg aacccgcacc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    23641 tgttgttggc cggggtttag acgcatagga aaatcgctca atgaaagtcg ggaggggaaa
    23701 ataacagcgt ggtagtatag ccagcctcat ccttaccact caaggcacga tcatggcaaa
    23761 aacagcagca gcactgcata tccttgtaaa agaagagaaa ctggctctgg atcttctcgt
    23821 gcagattaag aacggtgccg atttcggcaa gctggcgaag aaacactcca tttgcccatc
    23881 aggcaaacgc ggcggtgatt taggtgaatt ccgccagggt cagatggttc cggcgttcga
    23941 taaagttgtg ttctcttgtc cggtactgga gccaaccggc ccgctgcaca cccagttcgg
    24001 ttaccacatc attaaggtgc tgtaccgtaa ctaatagcaa ggccttctcc gggagaaggc
    24061 cttgaactct gaatcgctat taacccgcaa cagcaatacg tttcatatcc gtcatatagc
    24121 cgcgcagttt cttacctact tgctcaatcg catggctgcg aatcgcttca tttacatcac
    24181 gcagctgcgc gttatctacc gcaccttccg gaatagcttt acccaaatcg cccggttgca
    24241 gttctgccat aaacggtttc agcagcggca cacaagcgta agagaacaga tagttaccgt
    24301 actcggcagt atcagagata accacgttca tttcatacag acgcttacgg gcgatggtgt
    24361 tggcaatcag cggcagttcg tgcagtgatt catagtaagc agattcttcg atgatgccgg
    24421 aatcaaccat ggtttcgaac gccagttcaa cgcccgcttt caccatcgcg atcatcagta
    24481 cgcctttatc gaagtactcc tgctcgccga ttttgccttc atactgcggc gcggtttcga
    24541 aggcggtttt gccggtctct tcacgccagg tcagcagttt cttatcatcg ttggcccagt
    24601 ccgccatcat accggaggag aattcgccgg agatgatgtc gtccatatgt ttctggaaca
    24661 gcggtgccat gatctctttc agctgttcag acaacgcgta agcacgcagt ttcgccgggt
    24721 tagagagacg gtccatcatc agtgtgatac cgccctgctt cagcgcttcg gtgatggttt
    24781 cccaaccgaa ctgaattagt ttttctgcgt atgccggatc ggtgccctct tccaccagct
    24841 tgtcgaagca cagcagcgag ccagcctgca acataccgca caggatggtt tgctcaccca
    24901 tcaggtcaga tttcacttcc gcaacgaagg acgattccag cacgcccgca cggtgaccac
    24961 cggttgctgc cgcccatgct ttagcgatcg ccatgccttc gcctttcgga tcgttttccg
    25021 ggtgaacggc aatcagcgtc ggtacgccga atccacgttt gtactcttca cgcacttcgg
    25081 tgccagggca tttcggcgca accatgacta cggtgatgtc tttacggatc tgctcgccca
    25141 cttcgacgat gttgaaaccg tgcgagtaac ccagcgccgc gccgtctttc atcagtggct
    25201 gtacggtgcg cactacatca gagtgctgct tgtccggcgt caggttaacc accagatccg
    25261 cctgtggaat cagttcttcg taagtaccca ctttaaaacc attttcggtc gctttacgcc
    25321 aggacgcgcg cttctcggca atcgcttctt tacgcagagc gtaggagata tcgagaccag
    25381 aatcacgcat gttcagaccc tggttcagac cttgtgcgcc acagccgacg atgactactt
    25441 ttttaccctg aaggtagctc gcgccatcgg cgaattcatc gcgccccata aagcgacatt
    25501 tgcccagctg tgccagctgc tgacgcagat tcagtgtatt gaagtagtta gccatggtga
    25561 ttcctcgtga tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg aattcactat
    25621 atgacaggaa atttattgcg gaaattgata tattcacaac gtcacattgc aatttctgca
    25681 acgtcaacat tgagggctgc gccattggat ttacgcgatc tgaaaacctt cctgcatctg
    25741 gcggaaagcc gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc
    25801 tcacggcaga ttcagcgcct ggaagaagat ctcggccagc cgctgtttgt gcgcgataac
    25861 cgcacggtga cgctgactga agcgggcgaa gaactgcgcg ttttcgccca gcaaacgctg
    25921 ttgcagtatc agcagctgcg ccacaccatc gaccagcaag ggccgttgct ctatggcgaa
    25981 ttacatatct tctgctcggt gaccgccgct tacagccatc tgccgccgat tctggatcgc
    26041 ttccgcgcgg aacacccgtc ggtggagatc aaactcacta ccggtgatgc ggcagatgca
    26101 atggaaaaag tcgtcacagg tgaagctgat ctggcgattg ccgggaagcc agagactctg
    26161 cccggcgcgg tggcgttttc gatgttggag aatctggcag tggtgttgat tgcacccgca
    26221 ttgccctgcc cggtgcgtaa tcaggtttct gtcgaaaagc cggactggtc aacggtaccg
    26281 tttatcatgg ccgatcaggg gccggtgcgc cgccgcattg agttgtggtt ccgacgcaat
    26341 aaaatcagta acccgatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg
    26401 gtggcgctcg gctgtggcgt ggcattgttg ccggaagtgg tgctggaaaa cagccccgaa
    26461 ccggtgcgta accgcgtgat gattttagag cgcagcgatg agaaaacgcc gtttgagctg
    26521 ggcgtgtgtg cgcagaaaaa gcggctacat gagccgctaa ttgaggcatt ctggaagatt
    26581 ttgccgaacc attaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg
    26641 caatcgtagc ccagctcatt cagccgggtt tcgaaatccg gttcatggtc gccaagttcg
    26701 aacgccgcca gtacgcgccc gtagtcggtg ccatggctgc gatagtggaa caaagaaatg
    26761 ttccagtgcg tacccagcgt gttgaggaag cgcagcagcg cgcccggtga ttccgggaat
    26821 tcgaagctgt agaggcgttc ctgcaacgga tgtgacggac gcccgccgac catatagcgc
    26881 acatgcaact tcgccatttc gtcgtcggag agatccacca cgctgtagcc gccgtcgttg
    26941 agcatctgca aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag
    27001 atgcaggcgt ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca
    27061 agcagttggc agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac
    27121 gcttcacgct gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc
    27181 acgttggcac cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata
    27241 tattttttca ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc acgcacatct
    27301 tcgaataaat ccttcatcgc cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg
    27361 agatactcct ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg
    27421 gcgaacagtc ccacgcgtgg cagatcaacc ggatgacccg catccagcgc tgctttcagg
    27481 caggcggagt cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc
    27541 agcaccgcta cgcccgccgc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga
    27601 tgggcgtcct gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga
    27661 tggtcgaacg gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttagct
    27721 ttcgcttcgt caaagttcgc gccatgaagc agcacttcgc cgccgaatcc acgtaccgca
    27781 tcgactttga tgtcggcggt ggcggttggc ataacgatca gggccttcac gcctaaccgc
    27841 gcagaagaaa acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc
    27901 gctttttgtt cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg
    27961 tgcactggct ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt
    28021 ttttccattt tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact
    28081 gctcttaaat attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt
    28141 aaccccccag tttcgattta tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg
    28201 cgtaggcgca cagggcaaag gaaacctgac gttcacggtt tttcggcgtc caggctttgt
    28261 caccccgagc ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact
    28321 gaatgccacg gttcgggatg tcgatagcga tcaggtcacc atcttcaatc agaccaatgc
    28381 tgccgccgct tgccgcttcc ggtgagacgt ggccgatgga aagaccagag gtgccgccag
    28441 agaaacgacc gtcggtgatc agcgcacagg ctttaccgag acccattgat ttcaggaagc
    28501 tggttgggta gagcatttcc tgcatgccag ggccgccttt cgggccttca tagcggatca
    28561 ccaccacatc gcccgcgaca actttgccac cgaggatcgc ttctaccgcg tcgtcctggc
    28621 tttcgtacac tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt
    28681 tcacgatgca gccgttttca gcaaaattac cgtagagcac cgccaggccg ccgtctttgc
    28741 tgtaggcgtg ttccagcgag cggatacagc cgtttgcgcg atcgtcatcg agagaatccc
    28801 aacggcaatc ctgcgagaat gcctgtgtag tacgaatgcc cgcagggcct gcgcggaaca
    28861 tattttttac cgcgtcatcc tgggtcagca taacgtcgta ttgttccaga gtttgcggca
    28921 acgtcaggcc aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga tccagttcac
    28981 cgagaatacc gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc
    29041 tcggcgcaac tttgcataac tgcgggactt tacgcgaaag tttgtcgata tcactcatgg
    29101 tgaagtcgat ttccgcttcc tgcgccgccg ccagcaggtg aagtaccgtg ttggtagagc
    29161 cgcccatcgc gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc
    29221 gcggcagtgc actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt
    29281 taccggcatt gaggaacaac tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc
    29341 ccggctgcga caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc
    29401 cggagcagga gccgcaggtc ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag
    29461 aaactttcgg gtctgcgccc tggatcatcg catcaaccag atcgagtttg attatctgat
    29521 cggacagttt ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat
    29581 tcaggcgcag ggaagccatc agcatccccg gggtgatttt gtcgcagtta gagatgcaga
    29641 ccatggcgtc ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc
    29701 gagatggaag tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa
    29761 tggtgttgaa ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac
    29821 cgagatcgcg cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga
    29881 taatcggctt gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac
    29941 ccgccatatt acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac
    30001 tcccagtgtc tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga
    30061 ttaacttgat ctaaccagcc ccatttatct tcggtttcgc cagtgaagag gccgaagaag
    30121 gcttgctgaa tgcgtttggt aaccgggcca caacggcctt cgccaatctg aataccgtct
    30181 acgctgcgca ctggcgtgat ttctgccgcc gtaccggaca taaagacttc atccgccaag
    30241 tacagggatt cgcgcgacag cacctgctca cgcacttcaa ttcccagctc tttcgccagt
    30301 ttgatgatgg catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac
    30361 agcacaccat ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc
    30421 acatccagcg cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg
    30481 gaagagagat agttaccacc ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg
    30541 ttccaggagg aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct
    30601 ccccacggga aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttactccc
    30661 atgccaacat taccgacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg
    30721 cggatcacgt cacgacaagc ttccatcagc tcatcaatgc tctgtgaaac cgggaagcga
    30781 tagattttgg cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt
    30841 cctttgtgtg agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg
    30901 tgcgacatca catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg
    30961 taatcagctt tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg
    31021 tgatgtggtt gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg
    31081 actaaacagt aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg
    31141 tgcatcgctg gcggcagcca tattcattga gcagacgtgg aaaccacgat gacgcaccac
    31201 gcgtaaaaca cgttctaagg tttccggatt gaagcgagcc gatacattga cctgatgttg
    31261 catcatgata atttctccaa catttctgaa ttactggcac ctggcggcac cagcggccag
    31321 acgttctcaa gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg
    31381 gtgtcgagtg ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag
    31441 gcgctggcta acatgaggaa atcggggtta tcggtaaggg tggtttcgct gtatcgttcc
    31501 tgaaaaaaca gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg
    31561 attttcaacg gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa
    31621 gagccgtcac cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaact
    31681 gccgccggta aaccaaaacc catggtacct aagccgctgg aggtgatgaa attttccggg
    31741 cgagtgtggg cgatgtgctg cgcagcccac atctggtgct gccccacatc tgtggtcacg
    31801 acgcaatccg caggtttacg atccgacagt tgttttaaca acaacggagc gtagatagcg
    31861 tcaccgggat ggtcgtaacg ccaggaatgt tcatcacgca gctgcgcgca gtgttgctgc
    31921 cagtcattga tatttaacgg ctgctgtaat gctggtaaca gagcatttaa atcaccttgt
    31981 aatgccacat gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata
    32041 acactggcgt gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca
    32101 cccacggcga tcagcaggtc acactcctgc accgcgaagt ttgccgcttt ggttccgtgc
    32161 atccccagca tgcccagata gtacggataa tctgcttcta ctgcgcccag ccctttcagc
    32221 gtacaggtgg caggcatttt tgtggcagcg agaaattcac gcaaagccgg aactgcctgc
    32281 gccatcccca cgccaccgcc aacgtacagc atcggttttt gcgcttttgc cagcatctgg
    32341 cgcgcttgct cgacttcggc atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac
    32401 ggttccagat cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca
    32461 ggacgacctg agctggcaac gtcgaatgct tcagccatga tgcgcggcaa ctcttccaga
    32521 gactgcacca gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc
    32581 tgaaatgcgt cggtgccgat aaacggtgcg gacacctgac cagtgatggc aacgacaggg
    32641 atggaatcta acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac
    32701 gtggcgatac atacgccagt tttaccggta gcacgggcat aaccgatagc cgccattgcc
    32761 gcaccctgct catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa
    32821 accggcataa ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac
    32881 gcatgtacca cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac
    32941 agaattttgt gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaac
    33001 ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta agcctttcct
    33061 cgtccaagtg cagccccgca cggtgggata ataatcacca ccacgctaat cacgaccagg
    33121 ctaatcactc gtagaagggc tgtcattttg tcttttcttg catcttgttc gaaggaatgc
    33181 ctaaagagtt accatagatt ttgcgaatag cacaagatat ttttgggcct atttttatga
    33241 tggctttaat aatttcaata gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa
    33301 attcaatttc agtataaatg ttgttccctt cctcgtagtt catcctttgt ccgctgcatt
    33361 tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa
    33421 tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg
    33481 tgactcaagg agggtttatg tcactgtcaa ttgttcatac ccgcgcagcc ctggaagtaa
    33541 atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctacccggc ttaacgatgg
    33601 tgggcttaac agaaacaacg gtgaaagaag ctcgcgatcg cgtgcgcagc gccattatca
    33661 atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc
    33721 caaaagaagg gggacgatat gatttaccta tcgccattgc gttgctggcg gcctcagaac
    33781 agcttacagc caataagtta gatgaatatg aattagtcgg agaactagcg cttacaggcg
    33841 ctctgcgtgg cgttcccggt gcaatctcca gtgcaactga agctattaag tcgggcagaa
    33901 aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc
    33961 tgatagccga tcatctgcag gctgtctgtg cgtttctgga aggtaagaac actctcgaac
    34021 gcccgaaacc aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg
    34081 gtcaggagca aggaaaacga ggactggaaa ttaccgccgc tggtgggcac aaccttttac
    34141 tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc
    34201 cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg
    34261 aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt
    34321 taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ttggcgcata
    34381 acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc
    34441 gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc
    34501 cagcccgttt ccagcttgtt gcggcgatga atcccagccc taccggacat tatcagggaa
    34561 accataaccg ctgcacgcca gaacagacat tacgttatct caaccggctc tcggggccct
    34621 ttctcgaccg cttcgatctc tcactggaga tcccattacc accccccggc attttgagta
    34681 aaacggtagt gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg gccgccagag
    34741 agcgccaatt taagcggcag aataaactga atgcctggct ggatagtccg gaaatacgcc
    34801 aattctgcaa acttgagagc gaagatgcga tgtggttgga agaaacgctg atccatctgg
    34861 ggttatcgat tcgtgcctgg cagcggttac tgaaagttgc acgaaccatt gctgatattg
    34921 atcagtctga cattatcaca cgtcagcatt tgcaggaggc agttagctat cgtgcgattg
    34981 accgtttgct catccatctg caaaaactac tgacataaaa aaagggcatt tcgccctttt
    35041 tattaatcgt cagaatcggt gtagtcttca gcaccttcaa cctgcggttt accgccggaa
    35101 agggtgtgaa aacgttttgg acgcttaata cgcgtcatat acttggacca cacgcgttct
    35161 gcttctgtca ctggctcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg
    35221 gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc
    35281 agttgtgcct ctttgatggt gaaatcacca tgacgagaga atccacgtgg ataatgttta
    35341 ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct
    35401 ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac
    35461 atttaaatca taacgacaaa taattttgcg gagagcactg tggatacgga attgttaaaa
    35521 actttcctgg aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg
    35581 acccagtcag cagtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaacctt
    35641 ttcacccgcc acagaaacaa tatccgttta accgctgccg gtgaaaaact actgccttat
    35701 gcagaaacgc tcatgagcac ctggcaggcc gcccgcaagg aggtggcgca tacctcacga
    35761 cataacgagt tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg
    35821 ttgggacgct tgtatcaaaa tcaggatgcc catacaggct tacagttcga agcgcgaatt
    35881 gcccaacggc agtctctggt aaaacagctg catgaacgcc agcttgatct tcttattacc
    35941 actgaagcgc ccaaaatgga cgaatttagt agtcagttgc tggggtattt cactttagcg
    36001 ctttatacca gtgccccttc taaactaaag ggagatctta actatctgcg actggagtgg
    36061 gggccagatt ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt tcccattctg
    36121 acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg
    36181 ctacccgtca gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga tagcacaaca
    36241 ctttcacggc cgctttatgc catatggctg caaaatagcg ataaaaatgc gttgattcgc
    36301 gatcttttga aaattaacgt gctggatgaa gtgtattaat ctgaatggct ggcaaggatg
    36361 ccggtagaag gatttacttc ggagaggatt atttcagata aaaaaaatcc ttagctttcg
    36421 ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg gttttggaga
    36481 ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga cgggactcga
    36541 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga
    36601 attcttttac aaccaccggt tttatgaccg gcttac
//
LOCUS       DAJTNV010000033        35019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNV010000033 DAJTNV010000000
VERSION     DAJTNV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..835)
                     /locus_tag="OO199_001699"
     CDS             complement(<1..835)
                     /locus_tag="OO199_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5535771.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="OO199_001700"
                     /pseudo
     CDS             927..1013
                     /locus_tag="OO199_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1418..1894)
                     /locus_tag="OO199_001701"
     CDS             complement(1418..1894)
                     /locus_tag="OO199_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5535772.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="OO199_001702"
     CDS             complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="OO199_001702"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5535773.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3329..4369
                     /gene="bioB"
                     /locus_tag="OO199_001703"
     CDS             3329..4369
                     /gene="bioB"
                     /locus_tag="OO199_001703"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5535774.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4366..5520
                     /gene="bioF"
                     /locus_tag="OO199_001704"
     CDS             4366..5520
                     /gene="bioF"
                     /locus_tag="OO199_001704"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5535775.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5507..6262
                     /gene="bioC"
                     /locus_tag="OO199_001705"
     CDS             5507..6262
                     /gene="bioC"
                     /locus_tag="OO199_001705"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5535776.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6255..6932
                     /gene="bioD"
                     /locus_tag="OO199_001706"
     CDS             6255..6932
                     /gene="bioD"
                     /locus_tag="OO199_001706"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5535777.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7511..9532
                     /gene="uvrB"
                     /locus_tag="OO199_001707"
     CDS             7511..9532
                     /gene="uvrB"
                     /locus_tag="OO199_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5535778.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="OO199_001708"
     CDS             complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="OO199_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5535779.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10897..11041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11029..12018
                     /gene="moaA"
                     /locus_tag="OO199_001709"
     CDS             11029..12018
                     /gene="moaA"
                     /locus_tag="OO199_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5535780.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12040..12552
                     /gene="moaB"
                     /locus_tag="OO199_001710"
     CDS             12040..12552
                     /gene="moaB"
                     /locus_tag="OO199_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5535781.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12555..13040
                     /gene="moaC"
                     /locus_tag="OO199_001711"
     CDS             12555..13040
                     /gene="moaC"
                     /locus_tag="OO199_001711"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5535782.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13033..13278
                     /gene="moaD"
                     /locus_tag="OO199_001712"
     CDS             13033..13278
                     /gene="moaD"
                     /locus_tag="OO199_001712"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5535783.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13280..13732
                     /gene="moaE"
                     /locus_tag="OO199_001713"
     CDS             13280..13732
                     /gene="moaE"
                     /locus_tag="OO199_001713"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5535784.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13869..14573
                     /locus_tag="OO199_001714"
     CDS             13869..14573
                     /locus_tag="OO199_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5535785.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14778..15491
                     /locus_tag="OO199_001715"
     CDS             14778..15491
                     /locus_tag="OO199_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5535786.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15527..16483)
                     /locus_tag="OO199_001716"
     CDS             complement(15527..16483)
                     /locus_tag="OO199_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5535787.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="OO199_001717"
     CDS             complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="OO199_001717"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5535788.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17721..18482)
                     /locus_tag="OO199_001718"
     CDS             complement(17721..18482)
                     /locus_tag="OO199_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5535789.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18615..19025
                     /locus_tag="OO199_001719"
     CDS             18615..19025
                     /locus_tag="OO199_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5535790.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18987..20093)
                     /locus_tag="OO199_001720"
     CDS             complement(18987..20093)
                     /locus_tag="OO199_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5535791.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20104..21237)
                     /locus_tag="OO199_001721"
     CDS             complement(20104..21237)
                     /locus_tag="OO199_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5535792.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21230..22966)
                     /locus_tag="OO199_001722"
     CDS             complement(21230..22966)
                     /locus_tag="OO199_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5535793.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="OO199_001723"
     CDS             complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="OO199_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5535794.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="OO199_001724"
     CDS             complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="OO199_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5535795.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24857..26044
                     /gene="rhlE"
                     /locus_tag="OO199_001725"
                     /pseudo
     CDS             24857..26044
                     /gene="rhlE"
                     /locus_tag="OO199_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="OO199_001726"
     CDS             complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="OO199_001726"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5535796.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26853..29003
                     /gene="dinG"
                     /locus_tag="OO199_001727"
     CDS             26853..29003
                     /gene="dinG"
                     /locus_tag="OO199_001727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5535797.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29031..29993
                     /gene="ybiB"
                     /locus_tag="OO199_001728"
     CDS             29031..29993
                     /gene="ybiB"
                     /locus_tag="OO199_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5535798.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30134..31219
                     /gene="hcxB"
                     /locus_tag="OO199_001729"
     CDS             30134..31219
                     /gene="hcxB"
                     /locus_tag="OO199_001729"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5535799.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31448..31708)
                     /locus_tag="OO199_001730"
     CDS             complement(31448..31708)
                     /locus_tag="OO199_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5535800.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="OO199_001731"
     CDS             complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="OO199_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5535801.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="OO199_001732"
     CDS             complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="OO199_001732"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5535802.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33032..35019)
                     /locus_tag="OO199_001733"
                     /pseudo
     CDS             complement(33032..35019)
                     /locus_tag="OO199_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8297 a         8981 c         9467 g         8274 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt
     1321 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
     1381 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg
     1441 atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt
     1501 catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt
     1561 cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
     1621 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
     1681 cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
     1741 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
     1801 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
     1861 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     1921 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
     1981 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     2041 ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat
     2101 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     2161 acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc
     2221 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg
     2281 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg
     2341 gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc
     2401 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     2461 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     2521 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     2581 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     2641 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg
     2701 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     2761 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     2821 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     2881 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     2941 tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg
     3001 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     3061 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     3121 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     3181 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     3241 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac
     3301 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     3361 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     3421 tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg
     3481 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     3541 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc
     3601 aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc
     3661 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     3721 gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa
     3781 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca
     3841 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg
     3901 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     3961 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     4021 gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg
     4081 gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     4141 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     4201 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     4261 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc
     4321 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     4381 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg
     4441 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     4501 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg
     4561 gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg
     4621 catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt
     4681 atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt
     4741 attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg
     4801 cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc
     4861 tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg
     4921 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     4981 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     5041 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg
     5101 ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     5161 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt
     5221 gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga
     5281 gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc
     5341 ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc
     5401 acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc
     5461 gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa
     5521 taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga
     5581 tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca
     5641 cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc
     5701 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc
     5761 cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga
     5821 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     5881 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     5941 gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt
     6001 tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca
     6061 gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg
     6121 tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     6181 attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt
     6241 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     6301 aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag
     6361 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     6421 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     6481 ccttcgcaga acccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat
     6541 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     6601 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     6661 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     6721 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     6781 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     6841 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     6901 ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc
     6961 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     7021 aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg
     7081 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     7141 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     7201 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     7261 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     7321 taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     7381 cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat
     7441 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     7501 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     7561 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctagcgc accagacgtt
     7621 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     7681 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     7741 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     7801 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     7861 acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat
     7921 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     7981 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     8041 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     8101 cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     8161 tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac
     8221 tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     8281 acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa
     8341 caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     8401 ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     8461 accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt
     8521 cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg
     8581 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     8641 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     8701 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     8761 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     8821 ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg
     8881 aatggcggaa gatcttgccg aatatctcga agaacacggt gagcgcgtgc gttatcttca
     8941 ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt
     9001 cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct
     9061 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     9121 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag
     9181 cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     9241 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     9301 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     9361 tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca
     9421 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     9481 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     9541 cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat
     9601 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     9661 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     9721 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     9781 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     9841 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     9901 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     9961 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
    10021 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
    10081 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
    10141 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
    10201 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
    10261 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
    10321 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa
    10381 acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt
    10441 gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
    10501 attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
    10561 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
    10621 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
    10681 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa
    10741 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct
    10801 tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt
    10861 ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc
    10921 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
    10981 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
    11041 ctgactgatg catttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
    11101 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    11161 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    11221 gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc
    11281 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    11341 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    11401 gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    11461 atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    11521 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    11581 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    11641 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    11701 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    11761 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    11821 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    11881 gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    11941 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    12001 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    12061 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    12121 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa
    12181 gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    12241 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    12301 cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt
    12361 atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    12421 gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    12481 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    12541 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    12601 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    12661 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    12721 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt
    12781 gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    12841 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt
    12901 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    12961 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    13021 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    13081 gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct
    13141 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac
    13201 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    13261 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag
    13321 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    13381 caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    13441 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    13501 gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    13561 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    13621 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    13681 ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt
    13741 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct
    13801 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    13861 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccccgg gctggcttgc
    13921 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    13981 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    14041 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    14101 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    14161 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    14221 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    14281 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    14341 acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    14401 ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata
    14461 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg
    14521 acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt
    14581 tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac
    14641 aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat
    14701 acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc
    14761 taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc
    14821 acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg
    14881 caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac
    14941 caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca
    15001 acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga
    15061 ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg
    15121 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
    15181 gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat
    15241 taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca
    15301 ttatctcgca tggacgaaat aagctccttg aacaggcagg aaaatgccat agtgaagagt
    15361 tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg
    15421 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
    15481 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
    15541 catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca
    15601 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
    15661 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
    15721 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
    15781 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
    15841 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
    15901 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
    15961 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat
    16021 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
    16081 agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc
    16141 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
    16201 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
    16261 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
    16321 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
    16381 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
    16441 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt
    16501 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
    16561 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
    16621 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
    16681 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
    16741 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
    16801 gagattactg gaccctactg tcgcccagtg atcgaccatc aatgccactt tgccgtggag
    16861 cgggcggcgg cggtactcaa aaacctgaat gccgccttta accagatagt tatacagcaa
    16921 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
    16981 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
    17041 ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc
    17101 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
    17161 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
    17221 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
    17281 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
    17341 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
    17401 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
    17461 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
    17521 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
    17581 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
    17641 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
    17701 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
    17761 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
    17821 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
    17881 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
    17941 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
    18001 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
    18061 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
    18121 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
    18181 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
    18241 aaatcgctcc acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
    18301 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
    18361 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
    18421 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
    18481 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
    18541 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
    18601 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
    18661 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
    18721 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
    18781 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
    18841 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac
    18901 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
    18961 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
    19021 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
    19081 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
    19141 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
    19201 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
    19261 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
    19321 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
    19381 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
    19441 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
    19501 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
    19561 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
    19621 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
    19681 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
    19741 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
    19801 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
    19861 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
    19921 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
    19981 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
    20041 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
    20101 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
    20161 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
    20221 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
    20281 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
    20341 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
    20401 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
    20461 accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
    20521 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
    20581 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
    20641 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
    20701 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
    20761 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
    20821 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
    20881 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
    20941 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
    21001 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
    21061 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
    21121 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
    21181 cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc
    21241 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
    21301 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
    21361 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
    21421 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
    21481 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
    21541 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
    21601 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
    21661 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
    21721 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
    21781 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
    21841 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
    21901 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
    21961 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
    22021 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
    22081 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
    22141 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc
    22201 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
    22261 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
    22321 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
    22381 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
    22441 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
    22501 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
    22561 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
    22621 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
    22681 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
    22741 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
    22801 atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg
    22861 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
    22921 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
    22981 ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
    23041 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
    23101 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
    23161 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
    23221 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
    23281 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
    23341 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
    23401 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
    23461 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
    23521 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
    23581 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
    23641 cgatacccgg caagcatcag attatactgc gcctgtgcca ccgagacgcc cgctttcgcc
    23701 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
    23761 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
    23821 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
    23881 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
    23941 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
    24001 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
    24061 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
    24121 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
    24181 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
    24241 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
    24301 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
    24361 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
    24421 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
    24481 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
    24541 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
    24601 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
    24661 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
    24721 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
    24781 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
    24841 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    24901 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgcggt
    24961 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
    25021 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    25081 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
    25141 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    25201 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
    25261 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    25321 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    25381 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    25441 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    25501 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    25561 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    25621 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat
    25681 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    25741 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    25801 gcacgtcgtc aaccatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    25861 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    25921 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    25981 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    26041 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    26101 gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc
    26161 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    26221 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    26281 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    26341 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg
    26401 cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac
    26461 cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat
    26521 gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    26581 aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa
    26641 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    26701 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    26761 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    26821 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    26881 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    26941 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    27001 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    27061 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    27121 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    27181 ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca
    27241 ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    27301 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    27361 gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg
    27421 acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta
    27481 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    27541 cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag
    27601 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgcgc
    27661 cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    27721 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    27781 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    27841 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc
    27901 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    27961 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga
    28021 accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc
    28081 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac
    28141 acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    28201 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc
    28261 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    28321 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    28381 ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg
    28441 agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    28501 ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt
    28561 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg
    28621 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    28681 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    28741 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    28801 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    28861 acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat
    28921 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    28981 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    29041 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    29101 ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg
    29161 gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    29221 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    29281 tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc
    29341 tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg
    29401 tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg
    29461 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc
    29521 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    29581 tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc
    29641 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac
    29701 taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc
    29761 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt
    29821 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg
    29881 gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    29941 gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat
    30001 ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    30061 ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    30121 acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    30181 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca
    30241 tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta
    30301 tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga
    30361 ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga
    30421 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    30481 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt
    30541 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg
    30601 cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc
    30661 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    30721 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    30781 tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg
    30841 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    30901 gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat
    30961 catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    31021 gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga
    31081 agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca
    31141 ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg
    31201 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    31261 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    31321 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    31381 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc
    31441 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    31501 tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg
    31561 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    31621 gggatgccgt taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag
    31681 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    31741 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    31801 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    31861 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    31921 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    31981 actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    32041 tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg
    32101 ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc
    32161 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    32221 tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    32281 taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga
    32341 ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    32401 gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc
    32461 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    32521 aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca
    32581 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    32641 gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    32701 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    32761 cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt
    32821 tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca
    32881 ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    32941 aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    33001 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    33061 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    33121 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    33181 ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt
    33241 ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg
    33301 ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    33361 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    33421 caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc
    33481 gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat
    33541 cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    33601 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac
    33661 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    33721 taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca
    33781 gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg
    33841 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    33901 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    33961 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    34021 ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg
    34081 gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat
    34141 gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat
    34201 tactcacatc tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg
    34261 gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc
    34321 gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca
    34381 tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag
    34441 tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg
    34501 gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat
    34561 taagatacaa acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac
    34621 gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc
    34681 gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg
    34741 cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca
    34801 tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc cctttaatca
    34861 cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg cgaataccat
    34921 caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg ccagtggtgg
    34981 agttaccgtt ctcacccgca aaaaacgcac ccacgccgg
//
LOCUS       DAJTNV010000034        34950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNV010000034 DAJTNV010000000
VERSION     DAJTNV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..2261
                     /locus_tag="OO199_001734"
     CDS             <1..2261
                     /locus_tag="OO199_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5535803.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3346
                     /locus_tag="OO199_001735"
     CDS             2258..3346
                     /locus_tag="OO199_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5535804.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OO199_001736"
     CDS             complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OO199_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5535805.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="OO199_001737"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="OO199_001737"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5535806.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /gene="uxaA"
                     /locus_tag="OO199_001738"
     CDS             6643..8130
                     /gene="uxaA"
                     /locus_tag="OO199_001738"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5535807.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="OO199_001739"
     CDS             8213..8764
                     /locus_tag="OO199_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5535808.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OO199_001740"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OO199_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5535809.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OO199_001741"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OO199_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5535810.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12843)
                     /locus_tag="OO199_001742"
     CDS             complement(11845..12843)
                     /locus_tag="OO199_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5535811.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12922..13614)
                     /locus_tag="OO199_001743"
     CDS             complement(12922..13614)
                     /locus_tag="OO199_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5535812.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="OO199_001744"
     CDS             complement(13687..14190)
                     /locus_tag="OO199_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5535813.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="OO199_001745"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="OO199_001745"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5535814.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /gene="higB"
                     /locus_tag="OO199_001746"
     CDS             15696..16010
                     /gene="higB"
                     /locus_tag="OO199_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5535815.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="OO199_001747"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="OO199_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5535816.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OO199_001748"
     CDS             complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OO199_001748"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5535817.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OO199_001749"
     CDS             complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OO199_001749"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5535818.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21080..21499)
                     /locus_tag="OO199_001750"
     CDS             complement(21080..21499)
                     /locus_tag="OO199_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535819.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21477..21830)
                     /locus_tag="OO199_001751"
     CDS             complement(21477..21830)
                     /locus_tag="OO199_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535820.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21831..22151)
                     /locus_tag="OO199_001752"
     CDS             complement(21831..22151)
                     /locus_tag="OO199_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535821.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22285..23718)
                     /locus_tag="OO199_001753"
     CDS             complement(22285..23718)
                     /locus_tag="OO199_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5535822.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRTKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23781..24230)
                     /locus_tag="OO199_001754"
     CDS             complement(23781..24230)
                     /locus_tag="OO199_001754"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5535823.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OO199_001755"
     CDS             complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OO199_001755"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5535824.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OO199_001756"
     CDS             complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OO199_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5535825.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28688..29019
                     /locus_tag="OO199_001757"
                     /pseudo
     CDS             28688..29019
                     /locus_tag="OO199_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OO199_001758"
     CDS             complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OO199_001758"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5535826.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30858..32378
                     /gene="aer"
                     /locus_tag="OO199_001759"
                     /pseudo
     CDS             30858..32378
                     /gene="aer"
                     /locus_tag="OO199_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OO199_001760"
                     /gene_synonym="yqjI"
     CDS             complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OO199_001760"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5535827.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33432..34177
                     /gene="nfeF"
                     /locus_tag="OO199_001761"
                     /pseudo
     CDS             33432..34177
                     /gene="nfeF"
                     /locus_tag="OO199_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34231..34306)
                     /locus_tag="OO199_001762"
     tRNA            complement(34231..34306)
                     /locus_tag="OO199_001762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34271..34273),aa:Ile,seq:cat)
     gene            34431..34937
                     /gene="mug"
                     /locus_tag="OO199_001763"
                     /pseudo
     CDS             34431..34937
                     /gene="mug"
                     /locus_tag="OO199_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8542 a         8977 c         9179 g         8252 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacc gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccagaca gtcaggcggt cgaaattgct ggccataaag ttgatgtatt cttccgtcag
    20821 cagcgcaacg cccggaaagc cgcccatggt gttcgggccg tctggcaaca gatgaccatg
    20881 ccaggctccg agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt
    20941 catgtactgc ggcgcaccag tacggttaat cacgttttta taattgtcgg cgttagcggc
    21001 atgagcggaa aaacttatca gcagagcgca ggttactggc gttaaaatag ttttgatttt
    21061 catgagatta cagtccttat taccagtaga aatattgcat caggaagatt tggtcgctgg
    21121 gatcgttaga ggcatttttc atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc
    21181 cgccatattc gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt
    21241 cagggcgatg tttggcgtag gtgtaagcgg tggagagata aacgcctttt aacgcttcat
    21301 acatggcgct caccatcatc ccgctttcac ttcccggtac gtccagcccg tcggcacgtc
    21361 cggtatggtg ccaggcgcga gcggcaaagt taggggtaaa caggcccgtg agatagagtt
    21421 gtcctgtgcc gtcggtaatt tccagaccat tcatccaggt gatgccttcg gcaaggtcat
    21481 actggatgta accgttcatc cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac
    21541 ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg
    21601 ctataacgaa agttgccgct taagttctgt accttcagcg cggtcataat gtcgcgggta
    21661 ttgggaatga cgtagcccaa atcgggtgtg aagtcgcccc accattgcag gtcatccagc
    21721 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    21781 tagaaacggt taatcccacc ttcgaaacca ccccagctat ggtcaggcac ctaggcatta
    21841 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    21901 tacttcatct ccaggccacc ttcaatatag gtgccgtcgc tgtatttatg tttattctca
    21961 ccctctaaat ccatatatcc accaagccct aattcgccgt aaaaacgaaa tgaaggagaa
    22021 gatgtcgagg taattgcggg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct
    22081 cctgccgcag aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg
    22141 attaatttca ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa
    22201 ttataaaaat ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg
    22261 cctgctcatt ttttattagt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg
    22321 gcgctcttca gccgttaatc cctttatata tttgctgtat ttccaccacg caaagccgag
    22381 gaagataaca ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg
    22441 gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat
    22501 accggtgcgg cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggtgcgtaa
    22561 attcaggtaa gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt
    22621 aataatggta ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag
    22681 cgggatgacg atcagaaact ggatccatgc tgcgcgcgcc ggaacgccgt tttcgttaag
    22741 ttcgacggtt ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg
    22801 cgttgcggtc cacatcagca gggaaccgaa cattgcggta aaggacacca gaccgacaaa
    22861 gcgattcatc aacgcttccg gcagaccaaa atacgccgcc atgccgtgga atacctgcac
    22921 cgatccgccg gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga
    22981 gacggaatac agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt
    23041 cgaaccgcct ttgacgtcgt taacgtacac cgcgacggac tccgcgccac ctgcggccat
    23101 aaagatccag gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac
    23161 ggtgatcgga tcggcaggct gtacgccgcc aaccagtgcc gtacctgcca gtaaaatgta
    23221 ggagagcgtt aacagcagca tcagcgttga agtgacggag gtaattggcc ccagcatttt
    23281 cgccccgttg gtggaaaccc aggtggagaa ggcgaacagc accatactga taacggtggt
    23341 ggcaaccggc gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac
    23401 gcgcggcaac agcgaggtga aaaagaacag atttacgaac cagtaagtgt aggcagtaat
    23461 aaatgcccaa cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt
    23521 tttatttaac gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt
    23581 cgcgaggaaa aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt
    23641 gttaaagctg taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt
    23701 acgtttggta tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac
    23761 tccttttgcc gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg
    23821 agaaccactt ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa
    23881 ataacgattt gcccttcgtg gacctctacg gtttctccgc agccttttaa atattcacgg
    23941 tcagtttcat cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt
    24001 ttttgctgcc cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc
    24061 gcatcggtcg cggagtcatt ttccacacgg taggtcaatg aatcaccgat ggagtgggcg
    24121 acgccaggct gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg
    24181 ccagaggcgt aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct
    24241 cgctgtgcaa attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg
    24301 cggtagcttc tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc
    24361 agacgcgcca ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagtagct
    24421 ggtggtcgag gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag
    24481 cgtggatgtt ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca
    24541 ccagcagacc gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc
    24601 cgttgttctg cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat
    24661 cgatgatgtt agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg
    24721 ccacctgatc gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg
    24781 gatagtcgcc gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc
    24841 gccagatgta ggtacagcgc atcccgaagt caaacaccgg cggtgcaata actgtgcggc
    24901 tgatgatcaa cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca
    24961 tgatctgcaa atgattcggc tgccacaacc cttcgtactc ctgcttgtgg ttgtcgatca
    25021 tcggcttgaa gaagttgatc tttggctcgc gggtcagcag cgactcgcca tttacctggc
    25081 aggatgtcgg tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg
    25141 tgcagctcaa acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca
    25201 ctggctgcgc ggtgttttcc ttcagcggga actgataaga ggcgatagaa tgcccggctt
    25261 cgctgtagcg ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt
    25321 ccagctgcgg cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt
    25381 taatctgctg cgtcgcgagc gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt
    25441 catcaagcgt ggtaaaccac agtttatttt cgactttcaa ctcgccgcga gtcagcgcgt
    25501 ggatttttac cggcgcgata acctgtttgt actctttcag gcccgatccc ggcgtctgat
    25561 cggaatagat caaaccatca agacagaagt tatagttgtt gggatagtcg ccgccgaatt
    25621 tataccagac attgccgtgg tcgtcctgtg cctggatccc gtggtcgcac cactcccaga
    25681 cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc
    25741 ccggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt
    25801 attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca
    25861 cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt gtcatccagc gctttcgccg
    25921 catggtacat cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga
    25981 tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac aatgcgctcg acgtagactt
    26041 tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg
    26101 attcgacgtc ggtttccgcc atcacaaaca ggccgtagat atcacacagt tcgtaaaaac
    26161 gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact
    26221 ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt
    26281 gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca
    26341 ctttgatatc acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt
    26401 ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct
    26461 gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg
    26521 cgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag
    26581 gggaggcggc gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt
    26641 cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat
    26701 aaacatcgcg gaagatcccc gccgaccacc acatatcctg gtcttccacg taggtagagt
    26761 ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga
    26821 tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacgt
    26881 agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc
    26941 cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg
    27001 gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca
    27061 tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg
    27121 cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt ccactgactg cttaagggca
    27181 gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa
    27241 aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat
    27301 tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa
    27361 ttgctggggg gttagtaaaa tttttactaa aaagcagcag gtaaatgcat ttactttaac
    27421 tggatcacga aaataaacgg cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa
    27481 ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg
    27541 cagcgcgcga ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat
    27601 ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc
    27661 gttaacgctg ataagcgaaa tatcttgtgg gatgttcagg ccacgttcat gaattgcccg
    27721 cagtacgccg atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg
    27781 cgccagcatt tgttttgcca gttcataacc cgacgaactg gaaaaaccgc cgcgccagat
    27841 atcctcttcg cgtaccactt gtttcagtcg gccatattcc gcaaaggcga cctcacgaat
    27901 atccgccttg ccaggctcat cttcaccgcc aataaaacca atacgattaa cgccctggtt
    27961 gatatagaag tcgatgattt ctttactgat gcgtgccaga tcgatatcca ccgcatcgta
    28021 accgctgccg ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cgctggcagc
    28081 ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc
    28141 tggtaatccg ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt
    28201 ttcaatgccg tggcggatcg ccagatagta aggatcgttg atctccagct cctgctggta
    28261 gctgtagata gccagaatat ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct
    28321 actggtcttg tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt
    28381 cacattcaat gtcggatcgt catttaagac cctggatact gtcgccaggg atacgccagc
    28441 ttcgattgcg atgtctttta atgttgccat gtttaccttt tttactttcc ctgatgtgcg
    28501 cccccattgt agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc
    28561 tttgacgctg taacggtaac acagcggctt aggaatgtga tcacgcctgc attctccatt
    28621 tttagcctac aggattgaga aaaacttttc tatagtttag taaaatttta tccgaaggga
    28681 ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt ctggaaatgc gcgtcggaaa
    28741 gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg tacatcgtac aggttgatgt
    28801 ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt actacagcga agaggagctg
    28861 atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag cgaaaatgcg tggtgaaacg
    28921 tcggaatgca tgctgttgtg tgcggaaacc gatgacggca gtgaaagcgt gttgttaacg
    28981 ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa aagatcggat ggcgacgtcg
    29041 tatcgccatc cgatttgata ttacgcttct tcgacactta ctcgcatggc cgccagcgcc
    29101 ttacgcgccg ctttgatcac cagttcacac tgttcaatgg tcagtgtcag tggcggttca
    29161 atgcggatcg ttttggcgtt attgagcgtt ccggccacca gtacgcgctg gcggaacatc
    29221 tcgctggcaa agttatagcc gatttcgtta tcaacaaact caatcgccat caacatccct
    29281 ttaccacgcg cttcctgtac cagatcggga tattcccgcg ccagttgacg gaaaccgtcc
    29341 agcaacatat cgcctttttg ctcagcctga gccggtaagt tctgctccag caacacattg
    29401 atggtcgcca gcgccgccgc acaggccagc gggttgccgc caaaggtggt ggtatgcagg
    29461 aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg cgatggtcgc gccaatcggc
    29521 atcacgccgc cgccgagggc ttttgccaga cacagaatgt ccggctgaac gttctcatgt
    29581 tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt gtacttcatc gaggatcatc
    29641 agtgcgccga actcatcgca cagcttacgc acggcggtga gatagcccgg cggcggcaga
    29701 attacgccac cttcgccctg aatcggttcg aggatcaccg cagccacatc atcaccggtt
    29761 tttttgcact cattaagagc cgtgcgcatg gcttcgatat tgccaaacgg cacatgacgg
    29821 aaccctggca gcagtggcat aaacggtttg cggaaggtcg atttcgccgt ggccgacagc
    29881 gcgccaagtg atttaccgtg gaacgcgccg ctggtggcaa taaaagtaaa tttgccgcgc
    29941 ggcgactgat aagccttcgc cagcttcagc gccgcttcaa cggactcggt gccgctatta
    30001 cagaagaagc tgtatttcag tttaccgggc gttagcgcag caagggtttt cgccagcata
    30061 gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt gtttcgcaag ttgattctgt
    30121 acggcggaaa ccacaactgg attacggtgc cccacgttga aaattccaaa acctcccagg
    30181 cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat ttaaacctcc cgcttgccac
    30241 tctacggctc cgtaatcccc gccggcggta acagatttgc gatactctaa aaaccccgga
    30301 ttgacatgct ctttgaagta ttcaatcacc tctcggttaa gtgctttcat ctcctcatga
    30361 tccagcgttc gcttctcaat gagattcagg gcgtgggcgc tgcacgctaa agccgatgcg
    30421 ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc gtatcacatg ataccgattt
    30481 aagtattgca gggattgcgc cactccggct gcgatcgcga aaatcgggat aaacacaaat
    30541 gcggttatca tttgtgcaat atttaatgct atccgttaac atttgttgtt attttgcacg
    30601 gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat
    30661 tgcctgattt ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt
    30721 aaatcaaaga attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg
    30781 ttaaagataa ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac
    30841 ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat accccgctgg
    30901 cggacgatac cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg
    30961 acacttttgt gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca
    31021 tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa
    31081 aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg
    31141 tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc
    31201 gtacccgggc gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgttgaact
    31261 ccggacgtac cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta
    31321 aactgccttc attaccgctt cgctggcgga cgcgtggagt gatgaccctg atgtttatct
    31381 tgctggcggc catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt
    31441 tagtggtatt gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg
    31501 tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc
    31561 gcagcgatga gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt
    31621 ggctaattaa cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg
    31681 cgaaaggcac cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa
    31741 cggtggcgac catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg
    31801 ctgccgataa actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga
    31861 ccacggtgat caagacaatg gacgatatcg ccgacagtac ctagcgcatt ggcaccatta
    31921 cttcgctgat taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg
    31981 aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc
    32041 atttagccag ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg
    32101 ctgataaggt gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata
    32161 ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg
    32221 aacaggccga tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc
    32281 agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg
    32341 ccagccgact ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt
    32401 tggagcacaa tgcctgatgc gatgctggcg catcttatca ggcctgcggg gtgtgtagcg
    32461 ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc
    32521 ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg
    32581 atctttttta tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct
    32641 ttaaaattat ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa
    32701 cgcgctttga tgcgttcttc aatcatctcc acttgttcgc ggttttcttc cagccactgc
    32761 gcaccttgtt cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc
    32821 agcgactgct cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa
    32881 ttcccctggg ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc
    32941 gagagaatat ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga
    33001 ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt
    33061 ccgcagtgct cgtgttctga cttctcacct ttgcagcagc cctcatgccg tggctggcct
    33121 tcatgtttac aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga
    33181 tatatctaat ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt
    33241 ggaagcacct cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat
    33301 ggcactgaat gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc
    33361 atgattaaca atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga
    33421 aggcgataac aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct
    33481 tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc
    33541 ttggcggcga ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct
    33601 tctttcctca acctgacgct cactttgtgc cgccaacggt aacggaagag agcatcgtct
    33661 ggccggaagg accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc
    33721 atgaactggc gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa
    33781 acttacggtg gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct
    33841 gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa
    33901 acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga
    33961 ttatctcgcg caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt
    34021 agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat tacttcatct ggataaccgg
    34081 cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa gcggaacagt atgacccaca
    34141 gcgggtacgt gcagcggctt actggcacgc aaagtaactg atatataaaa agaaaaaggc
    34201 tgacgatttc tcgtcagcct ttgctatatc tggtggcccc tgctggactt gaaccagcga
    34261 ccaagcgatt atgagtcgcc tgctctaacc actgagctaa ggggccatgg tagcggatta
    34321 taaagtaact ccgtgtcgca atccagccat taccgcgcgc ctgctgtttt tataaacaat
    34381 gtattttcaa tccgttatac ttttcctggt gatgttagaa aggagtaaat atggttgagg
    34441 atattttggc tccagggtta cgggtcgtgt tttgcggtat caaccctggg ctttcatccg
    34501 ccgggactgg ttttcccttt gctcatccgg caaatcgctt ctggaaggtg atatatcagg
    34561 ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg gattatcgtt
    34621 gtggcatcac caaactggta gaccgtccaa cggtgcaagc caatgaagtt tcaaagcagg
    34681 agctgcacgc aggtgggcgt aagctgattg aaaaaattga agattatcag ccgcaggcgt
    34741 tggcgattct gggcaaacaa gcatatgaac agggattcag ctagcgcggt gcacagtggg
    34801 ggaaacaaac gctcaccatt ggttcgacgc agatttgggt gctgccaaat cccagcggtt
    34861 taagtcgcgt ttcactggag aaactggttg aagcgtatcg cgagctggac caggcgctgg
    34921 tagtgcgtgg gcgataaaaa acgtcaccgg
//
LOCUS       DAJTNV010000035        34466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNV010000035 DAJTNV010000000
VERSION     DAJTNV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..332)
                     /locus_tag="OO199_001764"
     CDS             complement(<1..332)
                     /locus_tag="OO199_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535828.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO199_001765"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO199_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5535829.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO199_001766"
     CDS             complement(1693..2073)
                     /locus_tag="OO199_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5535830.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO199_001767"
     CDS             complement(2087..2503)
                     /locus_tag="OO199_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535831.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO199_001768"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO199_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5535832.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO199_001769"
     CDS             complement(3504..3971)
                     /locus_tag="OO199_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5535833.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO199_001770"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO199_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5535834.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO199_001771"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO199_001771"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5535835.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO199_001772"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO199_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5535836.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO199_001773"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO199_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5535837.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO199_001774"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO199_001774"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5535838.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO199_001775"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO199_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5535839.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO199_001776"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO199_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5535840.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO199_001777"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO199_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5535841.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO199_001778"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO199_001778"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5535842.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO199_001779"
     CDS             complement(14626..15441)
                     /locus_tag="OO199_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5535843.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO199_001780"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO199_001780"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5535844.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO199_001781"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO199_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5535845.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO199_001782"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO199_001782"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5535846.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO199_001783"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO199_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5535847.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO199_001784"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO199_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5535848.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO199_001785"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO199_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5535849.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO199_001786"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO199_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5535850.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO199_001787"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO199_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5535851.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO199_001788"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO199_001788"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5535852.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO199_001789"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO199_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5535853.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO199_001790"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO199_001790"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5535854.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO199_001791"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO199_001791"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5535855.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO199_001792"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO199_001792"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5535856.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO199_001793"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO199_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5535857.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO199_001794"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO199_001794"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5535858.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO199_001795"
     CDS             31575..32189
                     /locus_tag="OO199_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5535859.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO199_001796"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO199_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5535860.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO199_001797"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO199_001797"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5535861.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8112 a         8706 c         9313 g         8335 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgccgctg agaaaaagcg aagcgg
//
LOCUS       DAJTNV010000036        34088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNV010000036 DAJTNV010000000
VERSION     DAJTNV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..578
                     /locus_tag="OO199_001798"
     CDS             <1..578
                     /locus_tag="OO199_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535862.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="OO199_001799"
     CDS             complement(575..1711)
                     /locus_tag="OO199_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5535863.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="OO199_001800"
     CDS             complement(1717..2040)
                     /locus_tag="OO199_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5535864.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="OO199_001801"
     CDS             2223..2366
                     /locus_tag="OO199_001801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535865.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO199_001802"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="OO199_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5535866.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO199_001803"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="OO199_001803"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5535867.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO199_001804"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="OO199_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5535868.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO199_001805"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="OO199_001805"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5535869.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="OO199_001806"
     CDS             complement(8959..9567)
                     /locus_tag="OO199_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5535870.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="OO199_001807"
     CDS             complement(9638..10723)
                     /locus_tag="OO199_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5535871.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="OO199_001808"
     CDS             complement(10726..11088)
                     /locus_tag="OO199_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5535872.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="OO199_001809"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="OO199_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5535873.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="OO199_001810"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="OO199_001810"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5535874.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="OO199_001811"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="OO199_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5535875.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="OO199_001812"
     CDS             complement(15380..15589)
                     /locus_tag="OO199_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535876.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="OO199_001813"
     CDS             15873..16235
                     /locus_tag="OO199_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5535877.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="OO199_001814"
     CDS             16779..17462
                     /locus_tag="OO199_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5535878.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="OO199_001815"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="OO199_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="OO199_001816"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="OO199_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5535879.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="OO199_001817"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="OO199_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5535880.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="OO199_001818"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="OO199_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5535881.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="OO199_001819"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="OO199_001819"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5535882.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="OO199_001820"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="OO199_001820"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5535883.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="OO199_001821"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="OO199_001821"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5535884.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="OO199_001822"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="OO199_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5535885.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="OO199_001823"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="OO199_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5535886.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="OO199_001824"
     CDS             complement(29422..29853)
                     /locus_tag="OO199_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5535887.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="OO199_001825"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="OO199_001825"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5535888.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="OO199_001826"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="OO199_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5535889.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="OO199_001827"
     CDS             32939..33898
                     /locus_tag="OO199_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5535890.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34088)
                     /locus_tag="OO199_001828"
                     /pseudo
     CDS             complement(33885..34088)
                     /locus_tag="OO199_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8930 c         8691 g         7997 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcgca
//
LOCUS       DAJTNV010000037        33923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNV010000037 DAJTNV010000000
VERSION     DAJTNV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(1..744)
                     /locus_tag="OO199_001829"
                     /pseudo
     CDS             complement(1..744)
                     /locus_tag="OO199_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            752..1258
                     /gene="tnpA"
                     /locus_tag="OO199_001830"
                     /pseudo
     CDS             752..1258
                     /gene="tnpA"
                     /locus_tag="OO199_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1276..1944)
                     /locus_tag="OO199_001831"
     CDS             complement(1276..1944)
                     /locus_tag="OO199_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5535891.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2247..2840)
                     /gene="tehB"
                     /locus_tag="OO199_001832"
     CDS             complement(2247..2840)
                     /gene="tehB"
                     /locus_tag="OO199_001832"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5535892.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2837..3829)
                     /gene="tehA"
                     /locus_tag="OO199_001833"
     CDS             complement(2837..3829)
                     /gene="tehA"
                     /locus_tag="OO199_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5535893.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3953..4933
                     /locus_tag="OO199_001834"
     CDS             3953..4933
                     /locus_tag="OO199_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5535894.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4928..5464)
                     /gene="rimL"
                     /locus_tag="OO199_001835"
     CDS             complement(4928..5464)
                     /gene="rimL"
                     /locus_tag="OO199_001835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5535895.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5527..5751)
                     /locus_tag="OO199_001836"
     CDS             complement(5527..5751)
                     /locus_tag="OO199_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5535896.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5891..7546)
                     /gene="opgD"
                     /locus_tag="OO199_001837"
                     /gene_synonym="mdoD"
     CDS             complement(5891..7546)
                     /gene="opgD"
                     /locus_tag="OO199_001837"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5535897.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7771..9114)
                     /locus_tag="OO199_001838"
     CDS             complement(7771..9114)
                     /locus_tag="OO199_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5535898.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9331..10254
                     /locus_tag="OO199_001839"
     CDS             9331..10254
                     /locus_tag="OO199_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5535899.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10292..11932)
                     /gene="trg"
                     /locus_tag="OO199_001840"
     CDS             complement(10292..11932)
                     /gene="trg"
                     /locus_tag="OO199_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5535900.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            12308..13677
                     /locus_tag="OO199_001841"
     CDS             join(12308..12788,12788..13677)
                     /locus_tag="OO199_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5535901.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKISTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            13779..13931
                     /gene="hokA"
                     /locus_tag="OO199_001842"
     CDS             13779..13931
                     /gene="hokA"
                     /locus_tag="OO199_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5535902.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(14118..14330)
                     /gene="cspA"
                     /locus_tag="OO199_001843"
     CDS             complement(14118..14330)
                     /gene="cspA"
                     /locus_tag="OO199_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5535903.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(14611..14901)
                     /locus_tag="OO199_001844"
                     /pseudo
     CDS             complement(14611..14901)
                     /locus_tag="OO199_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            15335..16045
                     /locus_tag="OO199_001845"
     CDS             15335..16045
                     /locus_tag="OO199_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5535904.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(16095..17069)
                     /gene="ghrB"
                     /locus_tag="OO199_001846"
     CDS             complement(16095..17069)
                     /gene="ghrB"
                     /locus_tag="OO199_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5535905.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(17173..17823)
                     /locus_tag="OO199_001847"
     CDS             complement(17173..17823)
                     /locus_tag="OO199_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5535906.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            17986..20319
                     /gene="bisC"
                     /locus_tag="OO199_001848"
     CDS             17986..20319
                     /gene="bisC"
                     /locus_tag="OO199_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5535907.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(20288..20728)
                     /locus_tag="OO199_001849"
     CDS             complement(20288..20728)
                     /locus_tag="OO199_001849"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5535908.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(20725..21288)
                     /gene="tag"
                     /locus_tag="OO199_001850"
     CDS             complement(20725..21288)
                     /gene="tag"
                     /locus_tag="OO199_001850"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5535909.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            21446..22144
                     /locus_tag="OO199_001851"
     CDS             21446..22144
                     /locus_tag="OO199_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5535910.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            22373..23581
                     /locus_tag="OO199_001852"
     CDS             22373..23581
                     /locus_tag="OO199_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5535911.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            23905..25596
                     /gene="eptB"
                     /locus_tag="OO199_001853"
     CDS             23905..25596
                     /gene="eptB"
                     /locus_tag="OO199_001853"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5535912.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            25688..25764
                     /locus_tag="OO199_001854"
     tRNA            25688..25764
                     /locus_tag="OO199_001854"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25722..25724,aa:Pro,seq:cgg)
     gene            26508..28115
                     /gene="dppA"
                     /locus_tag="OO199_001855"
     CDS             26508..28115
                     /gene="dppA"
                     /locus_tag="OO199_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5535913.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            28423..29442
                     /gene="dppB"
                     /locus_tag="OO199_001856"
     CDS             28423..29442
                     /gene="dppB"
                     /locus_tag="OO199_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5535914.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            29452..30354
                     /gene="dppC"
                     /locus_tag="OO199_001857"
     CDS             29452..30354
                     /gene="dppC"
                     /locus_tag="OO199_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5535915.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            30365..31348
                     /gene="dppD"
                     /locus_tag="OO199_001858"
     CDS             30365..31348
                     /gene="dppD"
                     /locus_tag="OO199_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5535916.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            31345..32349
                     /gene="dppF"
                     /locus_tag="OO199_001859"
     CDS             31345..32349
                     /gene="dppF"
                     /locus_tag="OO199_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5535917.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(32379..33650)
                     /locus_tag="OO199_001860"
     CDS             complement(32379..33650)
                     /locus_tag="OO199_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5535918.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         8410 a         8380 c         8517 g         8616 t
ORIGIN      
        1 gtccctgcgg atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg
       61 ccagttgttg ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt
      121 ctggtttttc tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgtggcgag
      181 tttagccacg ccagcatcca gcccgaccat tgatgctgaa gggtgagccg gagtggatac
      241 ttcactttct gtctgaatac tgatgtacca cgtaccgcag gactggctga cagtgacatt
      301 tttcacaaca cccgtgacct gacggctatt ccggtagcgc atccagccca gtttcggcag
      361 aaaaatacgg ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt
      421 ctgtcaccgc tttttgaatc ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc
      481 ccgctcaagg tctttcagtg actgttgcaa tggctgtgag ggagaatctt taagccattg
      541 cgtttcagtg gcatttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta
      601 tttattaccg gcctcatgat tctcattctg acgtgccaga gcacgattga aaacgaaacg
      661 acatgcgcct gcgaagcgcc tcatttcaca ctcctgttga ccacctggtc ttaactggaa
      721 tttaaatgcc tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac
      781 cgatatccgg cgtggaaggc attgtgtttt cctgatgcat gttcacctag tctttgtcac
      841 cagataccga cgccagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc
      901 aaatgtatgt gcttattttg aagctgaact ggttgaaatg gatggcgaac cagatcacgt
      961 ccatttgtta attaactaac ctcccaaact ggcgatatcc agtctggtaa acagcctcaa
     1021 aggcgtatcg ggtaggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta
     1081 caaaggcgtt ttgtggagtc ctggctattt tgccagtagc tgcggaggtg cgccaatatc
     1141 cgtcatccgc caatacattg aacaacagca aacacctggt caggtggaaa accgcgcctt
     1201 atatccccgc cctgaaggat ggggttttac ggcgcaccgg ataaaaagcg ccatctaacg
     1261 atggcgcgtc tggaactact ttttgttaac gtcaaacatg gtggcatcgg tcgccatgtc
     1321 atcaataact tgtttaatat tttcgaaagc cattggcgta ctttcgttat tcaggtcttt
     1381 accttcgccc tgacgaacga ctttgataac cggtttatta gtcgctgcat caatcagctc
     1441 accttcaaaa tagaggcgag tatccatggt acggtggcct gtagccattt gcgtccccgc
     1501 aaccactaat gcaacaggaa ccacttcata gaattgcagc ccttctttgc tggtatctac
     1561 accggtaatg gccccacgga aaatcagact acgcggccca gcggtggtaa ccactggttt
     1621 acgctgcgct atcgcttctt tcatttcggt gttggtatag ttcaaaattt tatccagaac
     1681 tttctgccct acctgggtag aaggttttgg taccggataa taagtgattg ggttccagac
     1741 gatgctgtca tatttgcttt gatcaaaact cgggtctacc caacgtaaaa caggtttacc
     1801 tgttgctgag gttgtttctt ttaaatcaga gtaattgttt aaaaaaccag aatatttatc
     1861 tggctgggtg attttagagg cacaaccaga cagagccaac aagccgcata aagctgcaac
     1921 tttcgcaaat gatgtggtac gcatgaatag tttcctgaga tgattatatt actttctaag
     1981 ttatagcaaa aagcgccgct tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt
     2041 tcagcccctt ttcagacgac atagtcaatg ccgaaaaagg ggctaagtca atcatcagta
     2101 gttaaatttc agtaagttaa gctgctaaaa aacttcgact actgtgatta atttcacatc
     2161 ccttcgataa aaaatatgct taccgaaaag aagaacagta aatcatctca gcgtggggaa
     2221 ttttcacgcg ttttgtgctt accgggtcat tttttacgtg ccagcatcgt ggcgaaacgc
     2281 agtttaatac gattaccgtt ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat
     2341 ttcaccctct cccagccttc gtaatatcga cgtaattctc cctctttgaa ggcaaacgtg
     2401 aagccgacgg tacatggata atcagcggta tccatcgccg ccacaatcag gttgtaacca
     2461 ccaggtttag tgcaacgttg catattggca atcaaccctg ggatggtttt agcctcaagg
     2521 aacatcagca ccacagtcga aagaataaaa tcgtactgtc catcaaatgt gaggttattc
     2581 agatcaacga cccgggtgtg taaattatcc agattttcaa tggatttaat gcgctcgacg
     2641 ttggcgatac tcatggcatt tttatcccat gcgtcaacat cataaccatt ggctgccagg
     2701 taaagactgt tacgaccatt gccacagccc agatccagcg ttttacccgg tttaaccact
     2761 ttcaccgctt ccagtacttc agagtgtgtg cgggttaatt catatttatc agtaaaatag
     2821 ttttcgtcac gaatgatcat tctttgtcct ctgctttcat taaaacggcg cgctcggttc
     2881 tgactaacaa ttttccctgc atcagaagcg caaaagtacg gatgagcagt attgcaataa
     2941 taaaattggt aaagataaac agcggcaccg ccagcgtatg gaaaaatcca ttatcgctgc
     3001 cactccccag atgcaaaccg gtggttgcca gtgcagatac gccgaacgag aaactccaga
     3061 atgaagcatt aaatggctgg gagagatacc atggcatcag acgtagcata aacagcagtt
     3121 gcagcagtcc ataaccgaaa agcattttcg ccagcgtgtc accctcgccg ccgttgacgc
     3181 tcagccaggc actacaggcc accagcgcag gagcgagctg aatgccgagt gatgtccgca
     3241 gtgccgtggg taattctccc gaactgcgca gacgctgcaa gatcaccggt tcaaggctta
     3301 gccatgagaa aacgcctgcg cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac
     3361 cacaggccat tgcgctgata aagttgttgg caactgtcgg cagatacagt ccaggcgtgg
     3421 tagcttcttc agggtgagat ccgcgccata atcccgcagt ttgccaggcg gcataagcca
     3481 actgaaccac gacaccaaaa ctgaacatgc acaccgccag tgggcgaaac cacggaacaa
     3541 aaccaatcgc caccagcatc gtcgttgccg gaaacaaact cacaaaactg ctcagcactg
     3601 gatggcgaac ttccgccagc acgctatgcg gaaagcgtat gagtcgggta ataaatgcgc
     3661 tagtcaataa tccccagatg atcatcgcca gaatcaccag cccatcccct aaccagtggc
     3721 tgacctgcca aacctggctg gcatagcgcc aggcaaatcc catccctatc gtccccaaca
     3781 caataccaaa gtagcctgcc ggcaaattga gcactttatc gctctgcatc ttcttactca
     3841 ttttgtttaa tttataaagt atatttgaaa tgcattttat ggaattttct cgagcatagc
     3901 cagagccgca gaatttgcta cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa
     3961 atatcgccta agtgaagaac agcgagcctt tagttatcag gaagatggca ctaaaaaaag
     4021 tgtgttatta cggcagatta tcgccattag cgattttaac gatgtgatag caggaaccgc
     4081 tggcggctgg atcgatcgcg aaacggtgtt agcacaggaa ggaaattgct ggatttacga
     4141 ccagaatgct attgcgttcg gcggaacggt gatttccggt aatacgcgta tcaccggcac
     4201 cagcgtgctg tggggagagg tttacgcaac ggataacgtc tggatcgata atagtgaaat
     4261 cagccagggt gcatatataa gtgacagtgt caccattcat gactcattag tttacgtgca
     4321 atgtagaata tttggtcacg ccttaattga tcaacattct atgatcgtcg ccgcacaagg
     4381 cttaacgccc gaccatcaac tcctgctcca aatttatgat cgcgcaaggg ttagcgcctc
     4441 gcgtattgtc caccaggcac aaatttatgg cgatgccgtc gtccggtatg cttttattga
     4501 acatcgcgcc gaagtttttg attttgccag tgttgaaggt aatgaagaga acaacgtctg
     4561 gctatgcgat tgcgctaaag tctacggtca cgcgcaggtg aaagctggca tagaagaaga
     4621 tgccattccg acgattcatt acagttcgca ggtggcggaa tatgccattg tggaaggtaa
     4681 ttgtgtgtta aaacatcatg tcttgattgg cggaaatgcc gtggtacgtg gtggaccgat
     4741 tctgctcgac gagcacgttg taatacaagg tgagagccgt attacaggtg cggtgataat
     4801 tgaaaaccat gtggagttaa ccgaccacgc cgtcgttgaa gcctttgatg gcgacacggt
     4861 tcatgtccgt ggtccaaaag tcattaacgg cgaggagcgc attacgcgaa cgccgctggc
     4921 agggttatta tgaatcgata atacgcgcgt ataagttcac atcatcatag gcatcattca
     4981 ggaactcagc ctgtttcagg caaccttcaa ggataaaacc attgcgcaaa gcgacctggt
     5041 tgctttgcgg attgtccacc cgacatttga tcacgaagcg tctaagttca ccagactgag
     5101 cgtaatgatg aatcaatgcc tgcagcgcct gagaaatgat cccctgcccc tgatgagatt
     5161 cgtccagcca gtagccgatt tcagcagttt tattcagtgg ttcaatacga ttaaacgaga
     5221 taacgccgat aagttcatct tctttgaaaa tcatgaacat tttggcatag ccgcgttgat
     5281 gcaacatcac attaccctgc accgtttttc gcgtgtcctc ttcactctga acgaactgcg
     5341 gccagtttag cgactgctgt aaccaggttt tatttttaca gattaactga taaagaggtg
     5401 tgacgtgact ttccgcaaca gcatgtaatt caagtgattc gcttactttt atcgtttcag
     5461 tcatcttatc tccagtcaat ccatgctgtt gttaaaaaag ccgcctggct ggcggcttag
     5521 gaaagtttac acctctttga cgctctcctg ctgcaggatt ttgagcatct catcttttaa
     5581 ctgtagcttt tgttttttca tgcggaccac ttccgcattg taccctcgac cgtcggaacc
     5641 ttcctttctg gcaatttcat gatcaagttt attgtgttta tcgaacaagg acataaagcg
     5701 aggattttcg tttttcagac gggatattaa atctcggtat tctggaaaca tacgcactcc
     5761 ctgttagtgg ctgacacggt gtatctgaaa tacacaccat cagattactc atttaactta
     5821 gggaaaaatg cgagcgggat cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa
     5881 gaaaaaacga ttaactcatc acgcggtcgt caacatactg acgtttatcc ggcgctggcg
     5941 ggaaatactg atacagccat gtttcactga tagcgtcccc ctgacaacgt agatacatcc
     6001 gcatatcgac cggatcagtg gagtccgaag tcggatacca gtcaaactga atacgataac
     6061 catcgatggg ttcaatatag agaatttcga tttgcttcgc ttccccactg gaaagcgtaa
     6121 tcaccggctc aatgcctttt ggcgcggcag ctttcagatc accaccaacg aaatcgacgg
     6181 caaaacgacg cgcccatttt tcgggatagt gttcacctgg cgcccaacct tccgggaaac
     6241 cgcccatgcc ggtacgcgtc gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg
     6301 cactccagta cagacgatac tggaatgcaa actcatcacc tgcttttaca gctttttctg
     6361 gctgccagaa gcagacaatg ttatccagcg tttcgcccgt tgttgggatt tccatcaggc
     6421 cgatggtgcc cttaccccac ttgttacgcg gttccaccca cagacttggg cgtttgttat
     6481 accagcccat aatgtcctga taatgggaga agtcacgatc cagttgcaat aaaccaaacc
     6541 ctttcgggtt gttgtcggtg taagcattga actgcaattt ttgcggatta ttcagcggac
     6601 ggcaaatcca ctcgccgttg ccccgccaca tggacagacg atcagagtca tgaatttgcg
     6661 gatgaattgt atcgcacatc cgacgttcat tagtaccgca gctgaacata ctggtcatcg
     6721 gcgaaatgcc cagctgttta atgtctttgc gcgcatacag gtgattttcc acatccataa
     6781 tcacctgact tttctcacaa tggatagtga acttataggc accagtaatg ctggcgctat
     6841 cgagcaacgc ataaacggta aaggtagttg cccccggttt taccgtatca aaccagaagg
     6901 cggtaaagtc ggggaactct tctttactgt cggtgtaagt gtcgatcgcc aggccgcggg
     6961 ccgacaaacc gtattgatat gtatcatcaa cggcgcggaa ataactcgcg ccgagaaatg
     7021 atactacatc acggcgcgcc agttcggggg ctttaaacac gcgaaaaccg gcaaagccga
     7081 gatcgctttg cccttctaat tgtttggtat caacacctgc atcgttgtat ttgaacaact
     7141 ccgggcgaaa gtgaatttca cgcgccagat gtgttgctgg atctacagaa aacatacgaa
     7201 cgcggcgacg gaatcccatt cccatatgga agaactgagc gtccagttga cggttctcaa
     7261 cgttatgcca gagcgatttt tcggcgtcgt attgaatact gttataagcc tgcggcgtca
     7321 ttgtcgccag cgtgtcaggt aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt
     7381 cgtgcgccat tgactgtaga atggagaagt caaaacgctg ggtttgcccg tcggcaatat
     7441 cagaatctgc cgcgaatgct gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca
     7501 cggcggccat agccattaaa cctttaataa atcgtctacg atccatacct gaaagtgagt
     7561 ccttctggtg ttgtgaatgg tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt
     7621 tatgaccgct cactctagac aatatttatt aataatccaa ttgttgaact gagagaatat
     7681 gcataaatcg gcattttttt tatgtcgatg ggatgagcct gattcgtttg taaaatcagg
     7741 caaatttttg caaaggcgga tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg
     7801 ttcttccgct tcctggtaaa gctggaactc atccaaaacc ggacaaccca acgcctgctc
     7861 aaatgcttcg cgactggcat taccgtgact gcgtgtctgc gtttcattac ccagatttga
     7921 ctggaagatc cccgccgcgc taacgggcaa gaaatcttca taggtgatgg gttgcgccac
     7981 tacccaacca cgttcaatta agggctgtgg atcgtctcca ggatgaatcg cctgacgatg
     8041 cgcctcaccc gaaggcgtca gacggtaccg gaaccatgcc aacccttgct gacgcattaa
     8101 aaactcactg tcaggaaaag tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt
     8161 atcctgcccg gttccagcgt tacgcagaag atcatcatac agttgtcgcc ctttcggcgt
     8221 taatgccacg ccacgctgct caatttcacc aaagcgcgcg gtatgcgtgc cctgtttctg
     8281 ccccgcaaac aacaccgtct cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg
     8341 tacctcgcgg cgcggcggcc cctcgatgag aattttgggt tcaattccgc attcaggcgt
     8401 catcgactgc acccggtcaa tatccagcgt acgtggcgtc aggtggttga tatggcatcc
     8461 aggaaaacag accacatcag caatcaaccg atgttcgttg tgcaaggcgc gataggtttc
     8521 ttcatctacc gttgctaact ggtgccagcg aaacgtttcc agggcttcct gcacaaactc
     8581 ctgtgcctgt gtttcgttaa aaccgccctg ctgctcatat tcctctaaca gttgtcgaca
     8641 acgtggggtg aagatatcgc gctgacgcag aatctccgcc gctttctggc gcaaaatttc
     8701 gttctcgata agctcaaggc ggagtaagga tgtaaaaacg cggaagggat tacgcgccag
     8761 agaagcatca tcaatgggcc gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag
     8821 atcgtaatag ctcaccgggt acatccccat aatggcaaac atccgccgaa gagtagcaag
     8881 ctcttgtgca gtcccaacgc gaatcgcccc atgacgttca acattcagtc gcgccagctc
     8941 gtctgcattt accatttttt cgtgcagttg aggattgttt tccagcacag ccagattcac
     9001 atcagctacc agttccagca gcgtgccata ttgcggaact tcttgctggt acatggctga
     9061 cattgcctgc gaaaactgtt cccgaatctc atccgccgtg atgctgttcg ccatagtgtc
     9121 tgcctccagt ggtcagtaat ctggagtgta ggtaaccgca ttcactctgg cgggaagaat
     9181 ttacaaactg tgatctcgcc gcgaaaacat caatattatc cattttgctg taacataatt
     9241 gctttaatta ttaataatat tttgcaatca agttatcata atcaaacaac ttcacttgtc
     9301 agcgacaccg cttcgttttt aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc
     9361 atccgtttgc gccaccttca tacattcgta gctgtcgcac aacaaggaac tttggggcgc
     9421 gcagctgaaa cccttaattt gagtcaacct gcgctctcta atacattgaa tgaactggag
     9481 cagctgacgg gcgctcgctt gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc
     9541 ggcgaacaat ttttaacgca tgcagtcaga gttcttgacg ccatcaacac tgccggacag
     9601 tcgcttcatc gtaaagaagg tcttaataat gatgtcgtca gggttggtgc actacctact
     9661 gcggcactgg ggatattacc ttcggttata ggtcagtttc atcagcaaca aaaagagacg
     9721 accttgcaag ttgcgacaat gagtaaccct atgattctgg cgggtttaaa aaccggggaa
     9781 atcgatatcg gcattggtcg gatgtcagat cctgaactga tgaccgggct taattacgaa
     9841 ctgctgtttc ttgaatcgtt gaagctggtt gtccgcccta atcacccgct acttcaggag
     9901 aacgtaacgc taagccgggt gctggaatgg ccggtcgttg tatcaccaga aggcactgcg
     9961 ccacgccagc attcagatgc attagtgcag agccagggct gtaaaattcc ttcgggttgt
    10021 atcgaaacgc tgtctacttc gctatctcgt caacttaccg ttgaatacga ttacgtgtgg
    10081 tttgtccctt ctggcgctgt aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct
    10141 gttccgggac atggtgcagg cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc
    10201 tcttctggtt gccagttgat gattaacgct attcgaaaat caatgccgtt ctgaaaggtg
    10261 aagggatctg tcgatccctc cttgaacatt ttcacaccgt agcgaaacta actggttcac
    10321 ccgctccgcg aggttctgcc gacacagaat gtttgttcag acggaatacg ttcaccgcct
    10381 cagttaatcg tgcagcctgt tcttcaaggg acaccgccgc tgctgaggcc tcttctacca
    10441 gagaagcatt ctgttgcgtc accttatcca tttcagaaat cgcctggcta acctgagtta
    10501 tgcctctact ttgttcatcc gaggcggcgg cgatttcctg catgatatgt gtgacactcg
    10561 cgacggcatc aacaatagtg ctcatggttt tccctgccgt tgccacctca tccgacccca
    10621 ggtcaattaa cctgactgat tcactgatca agccttcaat ctctttcgct gcctgagcgc
    10681 tgcggcttgc gagtgtccgt acttcgctgg cgacaacggc aaatccacgc ccttgctcac
    10741 ccgctcgcgc ggcttcaacg gcagcattca gtgccagaat attcgtctgg aaagcaatac
    10801 tgttgatgac ggcggtgatc tcagaaattt tcttcgaact cgtggagata gcgcccatcg
    10861 tttttactac accggaaacc gtctgcccgc catcgctggc tttaatagaa gcctcttgcg
    10921 ccagtttgct ggcatgatgc gcgttatccg cattctgttt caccgtcgca gtgagttgct
    10981 ccatgctggc ggcagtttgt tcgatagccg ccgcttgttc ttcggtgcga gatgacaggt
    11041 ccgcattgcc agctgaaatt tcgctggtgc cacgataaat ctcttccgcg ccctgtcgaa
    11101 cagtccctac tgtcatcccc agtgaatgct gcatttgctg taaatgacga cttaagcgac
    11161 cgatttcatt acgacccgcc ggttcatcat tcatcgtcag atcgccactg gcgatttttt
    11221 caatccgttg tgcggcatgt tgcagtggac gaatgacgat ccgacgtagc accataaatg
    11281 ttatcagcgt catgaccagg gcaagcacaa acgcgccaat catgaacatc ccacccaggc
    11341 gggtgcgctg atgggccagt tccgccagtt ggttggctct ggtgctacgt atcttaacgg
    11401 ctttgttcaa aatatcggta taagcattat ccagcggtcg gatctgctca ctttcatgat
    11461 tgataatcgc ttcaaacatg ccatttttgg catatttcaa cataggttgc atacccgtga
    11521 tataagcctg aaagcgttga tttaattcag tgtcgaaggc ttcatcagca ggtgttttca
    11581 ccggtcgatt ctgataagca cgataacctt gctgcgactg tttaatctcc gattcggctt
    11641 gcgcaatatt tcgcttcatt gcttccattt ccgcaatacg actcgcagcc ccggcctgaa
    11701 tcatattgat ccgggctgaa cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct
    11761 caatctccgc agtaacatca cgctgatcgc gatcggcctg ccagaggaaa tagccagcca
    11821 gagcagaact taatgcgaat agaactaaga taccgccaag aatgcaggca aataacggaa
    11881 ccagcctgat gtgatgcaaa aaacctaatc gctgtgaggg agttgtattc attaccgttg
    11941 tctctcgtcc aggtttactc ctgatagaaa gtcatcggca cttgacggta attacttatg
    12001 accaaaaacc tctttgcgtc acatttttac aacaattatt gcagagaaaa ttaatgctat
    12061 tggaatgaag agagtaacga atggctaaac tggctaaccc gaatgacgaa aaattctgga
    12121 aaacagcacg caatttcgtg aagtgattat tttcgtccct aaggaaacct cataggatgc
    12181 ctgcgggtgc ctgagacttt ctgttttcag gctaaccggg aatcagaaag acgaaggccc
    12241 cgaccgatat ttctatcaac tgtactgcac ccattttgtt ggacgatgaa atggaatagc
    12301 ccctaatatg tcaaagccaa aatacccttt tgaaaagcgc cttgaagtcg tgaatcacta
    12361 cttcactact gatgatggtt acaggatcat ctcggcacgt tttggtgtcc cccgaaccca
    12421 ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggtttaa ttcccaaacc
    12481 taaaggcgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg tgatcgagca
    12541 gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg gttctgtagc
    12601 caggtggctg aaggtctatg aagagcgcgg agaagctggt ttacgcgcgc tcaagattag
    12661 caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag cattggagct
    12721 gtcaaaagac cgacgcattg aggatcttga aaggcaagtt cgatttcttg aaacgcggct
    12781 tatgtatcta aaaaagctga aagccttagc tcatcccacg aaaaagtgaa agtactcaac
    12841 gagctaaggc agttttatcc tcttgatgag cttctcaggg ctgcggagat accgcgcagt
    12901 acgttttatt accatctaaa ggctctcagc aagcctgaca agtatgcgga cgttaaaaag
    12961 cgtattggtg agatttatca cgagaataga ggccgatacg gataccgtag ggtaacgctg
    13021 tctcttcatc gagaagggaa acagattaac cataaagctg ttcagcgcct gatgggaacc
    13081 ctctcactta aagcagcgat taaggtcaag cgataccgct cttacagagg agaggtaggg
    13141 caaaccgccc ctaatgttct ccaaagagat ttcaaggcta cgcggccaaa cgagaagtgg
    13201 gttaccgatg ttactgaatt tgcagtcaat gggcgcaagc tgtatttgtc tccagtaata
    13261 gatctcttca acaacgaagt tatttcttac agcctttcgg aaagaccagt gatgaacatg
    13321 gttgagaata tgctcgatca ggcattcaaa aagcttaatc ctcacgagca tcctgttctg
    13381 cactctgacc agggatggca gtatcgtatg agaagatatc aaaatatcct taaagaacat
    13441 ggtattaaac aaagcatgtc cagaaaaggc aattgtctgg ataatgctgt ggtggagtgt
    13501 ttctttggaa ccttaaagtc ggagtgtttt tatcttgatg agttcagtaa tataagcgaa
    13561 ctgaaggatg ctgttacgga atatattgaa tactataaca gcagaagaat tagcctgaaa
    13621 ttaaaaggtc tgactccaat tgaatatcgg aatcagacct atatgcctcg tgtttaactg
    13681 tccaactttt tggggtcagt acaaagtgag gctaccctat gcctgaacac cataaggtta
    13741 gcctcttact cgttggaaat caacacaggg ggctgggaat gccgcagaaa tatagattac
    13801 tttctttaat agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac
    13861 tgtgtgaatt gcatattaaa caggagagtt atgagctggc ggcgttttta gcctacaaat
    13921 tgaaagagta agagtcttcg gcgggaaatt attcccgcct tacttacggc gttgcgcatt
    13981 ctcattgcac ccatatttat tcttcacaaa aataataata gattttatta cgcgatcgat
    14041 tatttatttc ctgaaaaaaa ataaaaaaat ccccgccaaa tggcagggat cttagattct
    14101 gtgcttttaa gcagagatta caggctggtt acgttaccag ctgccgggcc tttagcgccg
    14161 ctttcgatgg tgaaggacac tttctgacct tcgtccagag atttgtaacc atcgttctgg
    14221 atagcagaga agtgtacgaa cacatcttta gagccatcgt caggagtgat gaagccgaag
    14281 cctttgtcag cgttgaacca ttttacgata ccagtcattt taccggacat agtgtattac
    14341 ctttaataat taagtgtgcc tttcggcgat atggcgtgct ttacagattt tgaagcgtta
    14401 aaggaatgtg cactacgagg ggtatcaacg ataactcttg aagggacttg ccttactaca
    14461 ctgctttaat ggtctgtacg tcaaaccgtt gatgtgcatt aagccactca ttggcgggtg
    14521 atgcaacaat tatttttcat atttatgatt aatcggttgg aaaacggtcc tgtcatcagg
    14581 accgtaaaca gcaataaagt ggataaaagt ctatttcatc aactgcttac ttaatgccgg
    14641 gttggcttga atcaaacgca tcaattttag ttcggcactt gaaggcttca cgcgtctgga
    14701 ttcccattcc tttaccatgg cgactgatac gcccaaaacc cgggcgaaat catcgatttt
    14761 taatcctgtc ccttttcata actgctcaat ttcggtacag gacgttgttc tgtgcgtcag
    14821 ggtaattttc tgcgtttcat ctttaaaaac aatctgttcc aggctgctca acagctcatg
    14881 cattggatct ttatattcca ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc
    14941 atcgaagctc attaagaata gtcgggaaaa gcagacacag cggggtgaag tcagggcatg
    15001 ctctggctat gattcgattc agcctgtcat ttagcgtgac ttatagccac ctaacgatct
    15061 gataaatgcc gatatatctc ttatcactcc tgttttgtaa agatttcgca aatctaacct
    15121 tctgcgaagg cgtaaatctc tggctacaaa aaaggcaacg gcactatcat tttttaacaa
    15181 tgaaacaaca aattaacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag
    15241 aaagggtatc ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg
    15301 ttgtgcgctt tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg
    15361 ctggtttgcg cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa
    15421 agaaggcgat cagcgtaaag cgtttatcga cttcctgcaa aatacagtga tgcgtagcgg
    15481 tgaacgtcta ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga
    15541 ttacgcgatt ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg
    15601 cccggtcgta gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg
    15661 tccgctgcgt gaaatgaatg gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa
    15721 gttgcaggcc gatgctgcgc actctgcgct aaaacagagc gatgacctga aaccggtatt
    15781 tgatcaggca ttcaccaaag tggtaacgac gccagctgat gcattgcaac cgttgattcc
    15841 ggcggcacaa actttcacgc aacaactggt gatggttgga gactacattg ctcagcaagg
    15901 tactcaggtg agctttgtcg caaatggcat ccagttcccg acttcacagc aggcaagtga
    15961 atataacaaa ctgattgcgc cattaccggc acaacatcag gcgtttaatc aggcctggac
    16021 tacagcagtt actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactt
    16081 cacgcagtcg cagcttagtc cgcgacgtga ggattcacac agttcttctc aacctttcct
    16141 tgtaacgcat caatcaaatt atccacggca caggcggcca tgccataacg cgtctcatgg
    16201 gtggcagatc caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct
    16261 acggacagtg gctcttgttc gaagacatcc agcccggcgg cgtgaatttc gcctttctgc
    16321 aatgctgcga tcagtgcatt ttcgtcaacc accgggccac gtccggcatt aatgaaaatg
    16381 gcggaggatt tcattttggc gaattgttct gcgccaaaca gatgatgcgt ctcatcagtt
    16441 aacggcagga tcaggcaaac gaaatctgac tcttgtaaca gagtatccaa atcgcagtag
    16501 cgggcgttga agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc
    16561 atgttgaagc caaagtgcac acgttgtgcc agcgccatgc cgatccgtcc catcccgaca
    16621 atgcccagtg ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc
    16681 cattcgcctg cttttacccg ttctgctacc tccacaaccc gacgagcggt agacaacacc
    16741 agcgccatca gcgtatcggc gacggtttct gttaatacgg ttggcgtgtg catcagcaga
    16801 atttttcggg cggtaagcgc atcgacatca aaattgtcat agccgacgga gatcgttgat
    16861 gtggcacgca gtttcggcat tttttccagc aatgcggcat ctacattctc gtttgaaccc
    16921 agtaaacctt cagcttctgc aaaaattgct gcattttgaa cgacggtttg tgggctgagg
    16981 tttgccacct ggtgaacggt gaaatgcgct tgcaggcgtt gcagtaaatc atcaggtaag
    17041 gctttgtaga ggataacgga cggcttcatg cttctctcca ttcactgata aggcgatcac
    17101 cttagcctga aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg
    17161 gcatgaaagg gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc
    17221 ttctgcggtg ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg
    17281 gctggcgtcc acgccctggg tgatcaacgc gctggcaacg gaatccgcac gctgctggga
    17341 gagacgcatg ttcaggtcgt gaccccccgt gctgtcggta taaccaatca cgttaaccgc
    17401 cgttttcgga tactctttca gcaccattgc cacgccggtc agggtgttag cgcctgccgg
    17461 tttcagggga gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt
    17521 atccccgctg cgggttacgc taacaccagt gccgcgcatt ttgtcacgca gcttcgcttc
    17581 ctgcacatcc atgtaataac ccacgccgcc gcccagagct gcgcctgctg ctgcgccaat
    17641 cagcgcgcct ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgcccac
    17701 gagagagccc agacctgcgc cgatagcaga tttgcctgct tcgcgttcgc cggtgtaagg
    17761 gttagttgtg cagccagata ccgccagagc gccactcact acggcggcaa gtaagataaa
    17821 cacgtttctt cattgttaat ccttaataac ctttttattc tttgtcacgg gttccgtggc
    17881 gggagattat gccgcgtgaa catgaagatt attcctggga atactcggaa atttgtaagt
    17941 aatatttaac tgctcaatac atctaacctt tcaggagtct tcggtttggc caactcatcc
    18001 tcacgatatt ccgttctgac tgccgcccac tgggggccca tgctggttga aaccgacggc
    18061 gaaaccgtgt ttagctcgcg tggcgcgtta gccacaggaa tggaaaactc cttgcagagc
    18121 gcggttcgcg accaggttca cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc
    18181 tttctcgcgt caccggaaaa cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg
    18241 agttgggatg aggcgctgga tcttattcac caacaacata aacgcattcg tgaggcttat
    18301 ggtccggcat cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag
    18361 gcctcgacat tattacaacg ctatatggcg ctggcaggcg gttataccgg gcatctgggg
    18421 gattattcga ccggtgcggc acaggcgatc atgccgtatg tcgtaggtgg cagcgaagtt
    18481 tatcaacagc aaaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg
    18541 agtgctaacc cactcaatac gctgaaaatt gcgtggaatg catccgatga gcaggggctt
    18601 tcttactttt ccgcactgcg tgacagtggg aaaaagctga tctgcattga tccaatgcga
    18661 tcggaaaccg tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcacc
    18721 gatgttgcgc tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa
    18781 gcgtttctgg cgcgttgcac cacaggttat gccgtcttcg cctcttattt gctgggcgag
    18841 agtgacggaa tagcgaaaaa cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg
    18901 aaaatccgcg agctggcggc tattttccac caaaatacca ccatgctgat ggcaggctgg
    18961 ggaatgcagc gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcagca
    19021 atgttggggc aaatcggcac acccggcggc ggttttggtc tttcttacca ttttgccaat
    19081 ggtggtaacc ccacgcggcg ttctgcggtg ctctcttcca tgcagggcag cttgccgggt
    19141 ggctgcgatg cggtggataa aatccctgtt gcccgcattg ttgaagcact ggaaaaccct
    19201 ggtggcgcat atcaacacaa cggtatgaac cgacatttcc cggatattcg ttttatctgg
    19261 tgggcgggcg gtgccaactt tactcatcat caggatacca atcgtctgat ccgtgcctgg
    19321 caaaaaccgg agctggtggt gatctctgaa tgcttctgga cggcggcggc gaaacacgcg
    19381 gatattgttc tgcctgcgac tacctctttt gagcgtaatg atctcaccat gaccggtgat
    19441 tacagtaatc agcatctggt gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt
    19501 aatgattttg atgtttttgc cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg
    19561 tttacggaag gaaaaagtca gctgcaatgg ctggaaacgt tttataacgt tgcccggcag
    19621 cgcggggcaa gccagcaggt tgaattgccg ccatttgctg agttctggca agccaaccag
    19681 ttaattgaga tgccggaaaa cccggacagc gagcggttta ttcgcttcgc tgatttttgc
    19741 cgcgatccgc tggcgcatcc gttaaaaacc gccagcggca agattgaaat cttctcacag
    19801 cgtattgccg attacggtta cccggattgc cctgggcatc caatgtggct ggagccggac
    19861 gaatggcagg gcaatgccga accagaacag ttgcaggtac tttctgccca tccggcgcac
    19921 cgcctgcaca gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt
    19981 gagcctgtca ccattcatcc tgacgatgct caggcgcgcg gcataaccga aggggatatg
    20041 gtgcgggtgt ggaactcccg tgggcagatc cttgccggag cggtcattag cgagggaatt
    20101 aaacctggcg tgatttgcat tcacgaaggg gcatggccgg atctggattt aaccgctgac
    20161 ggtatttgta aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg
    20221 gggaatggct gtgcgggtaa tacgacgctg gcatggctgg aaaaatacaa cggtccggaa
    20281 ctgacactta cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt
    20341 catcctgcca tgcgcaatcg acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg
    20401 gttgattttt ttgataaacc tccagcatca gatggggata gcgctgctgc acatactgca
    20461 tcagcgcctt accaataccg cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa
    20521 atcggccttc cataatgctg acaaaaccga gaagcttacc gtcctcttcc cagacccagt
    20581 tttgcgcgtt ggcaagatag gcatcccgca ccagcggaat gcagtcacgc cagtaattcg
    20641 cttttataaa gggatgcccc caggttgtac tttccagcca cagttcgagg atcgcgggga
    20701 gttctgaacg ttgcgcttcc cgaatcatgg tttatttccc agatagcaac agcagccaac
    20761 cacatgatca ttcaccagcc cacatgcctg cataaaggag taacagattg tggtgccgac
    20821 aaacttaaaa ccacgttttt tcagtgcctt agatagggcg tcggaggcgg acgtagatgt
    20881 gggaatttcg ctcaacgttg tggcttgtgt cacctgtggc tgatgattta caaacgacca
    20941 gacaaagtcg ggaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc
    21001 aataattgcc tgaattttcc ctcgatggcg gataatcccg gcgtcctgta ccagtctttc
    21061 gacatcctct tcctgcattg ctgcgacctt caccggatcg aactgatgaa agcaggcgcg
    21121 atagttttcg cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttcaaa
    21181 gcagatcatt tcgaacagtt ttttactgtc agtttcaggc acgccccact cattatcatg
    21241 gtaggcaata taaagcgggc cctgactcac ccagccgcaa cgttccatac tttccctcgc
    21301 tatgtacccg atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat
    21361 atttaataca gggctactat gcccgatatc cttcatacaa tgacaataat attgccgaat
    21421 tcggctcttc cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct
    21481 aagcctgctg gcgagcgtgg taatcagtgc cttttttctc aacacagctt acgcctggca
    21541 acaagaatat atcgttgata cgcaacccgg acattccaca gagcgttaca cctgggatag
    21601 tgatcatcaa cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc
    21661 gctgggactg gaagtcaatc tggcggaaga aactcctgtg gatgtgacca gcagtatgag
    21721 tatgggctgg aattttcctt tatatgaaca ggttacaacc ggcccggtcg cggcattaca
    21781 ttacgatggc acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctgac
    21841 cgatccgtta tggcatgcca gcgtgagtag cttaggctgg cgtgttgact cccggcttgg
    21901 cgatctccga ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa
    21961 ggcgcaatca ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt
    22021 caccgtaggc gctgatatgt tgctcaatca aaatattgcc gcctatgctg cgctaactca
    22081 ggcagaaaat accactaata atagcgacta tctgtatacg atgggggtta gcgccagatt
    22141 ttaacgtaac agtcacaatt gaaaccattg aataacaaca gttgtggcga tagtgggtgc
    22201 taacttacca aataataaat ttggtgaata attgtcgcgt cattcattcc tgaactaagg
    22261 catttcattc cgttctgatg gcatttcatg ccgtttttcc ccaagcataa aatgcacttc
    22321 gttatggttg tcggcagaga tttttccttt ttattactgc aggaatactg ccatgacacc
    22381 ttcaaattat cagcgtaccc gctggctgac actcatcggt actatcatta cccagtttgc
    22441 gctggggtcg gtttatacct ggagcctgtt taatggcgcg ctttctgcca agctggatgc
    22501 gccggtaagc caggtcgctt tctctttcgg cttgttaagt ctggggctgg caatttcgtc
    22561 ttctgttgcg ggcaaattgc aggaacgttt tggcgttaaa cgcgtcaccg tggcttccgg
    22621 cattttgctg ggattaggct tcttcctgac cgcgcattcc aacaacctga tgatgctgtg
    22681 gttaagcgcc ggtgtgctgg tcggtctggc ggatggcgcg ggttacctgc tgacgctctc
    22741 taactgcgtg aagtggttcc cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc
    22801 ttatggtctg ggtagcctgg gtttcaaatt tatcgacacg cagctgctcg aaacggtggg
    22861 tctggaaaaa acctttgtga tttggggagc gattgcgctg gtgatgattg ttttcggcgc
    22921 aacgttaatg aaagacgcac caaaacagga agtgaaaacc agcaatggtg tggtggagaa
    22981 agattacacg ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggtaatgtt
    23041 cctgactgcc tgcatgagcg gcctgtacgt gattggggta gcgaaagata tcgcccaaag
    23101 tctggcacac cttgatgtgg tttccgcagc caatgcagta acggttattt ccatcgctaa
    23161 cctttcaggt cgtctggtgc tgggcattct gtctgacaaa attgcccgta tccgtgttat
    23221 caccattggt caggtgattt cgctggtggg tatggcggcc ctgctgtttg caccattaaa
    23281 tgcagtgacg ttctttgcag cgattgcctg cgtggcattt aactttggcg gcactattac
    23341 cgtctttcca tcactggtca gtgagttctt cggcctcaat aacctggcga aaaactacgg
    23401 tgtgatttac ctcggtttcg gtatcggtag catttttggt tcgattatcg cctcactgtt
    23461 tggcggtttc tatgtgacct tctacgtgat tttcgccctg ctgattctgt cattggcgct
    23521 ttctacgacg attcgtcagc cagagcagaa aatgttgcgt gaggcgcatg gctcccttta
    23581 atgctcagcc gcgagctact gggcttcagc ttccgtgtgc atcaaacgca tcaacgaaat
    23641 gtataaacaa tgactcgtaa aatttgggcc agttaacagg ccctttttac tgctttttgt
    23701 aacgctaaag tctcttttca aacttgcatt tttgtaaatt tgtgcttcat gcacactctt
    23761 tccccacact ttttcgcttt gctgtggtct acttattcgc gcgtgtagat tttacttatc
    23821 tgactacctc cgcacttttc ccctgccggg cctgaaaagc cgctaagcag ggtgttatca
    23881 cctgtttgtc cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg
    23941 agctttttgc ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttatcgccgc
    24001 ttcggcagct atgcgcacga ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg
    24061 gccgccaccg tactggtgac cttcttttta ctacgtcttc tatcgctgtt tggccgccgc
    24121 agctggcgta ttctggcgtc gctggtggtg ctcttttccg caggtgccag ctattacatg
    24181 accttcctta atgtggtcat tggttatggc atcatcgctt ccgtcatgac caccgatatc
    24241 gacctgtcaa aagaagttgt tggtctgaac tttattctct ggttaatcgc cgttagtgca
    24301 ttgcctctta tccttatctg gaataaccgc tgtcgctaca ccttgctccg acaactgcga
    24361 accccagggc agcgtattcg cagcctggtg gtcgtcgtac tggcgggtat tatggtttgg
    24421 gcaccgattc gtttgctgga tatccagcag aagaaagtgg agagggcgac cggcgttgat
    24481 ttgccgagtt atggcggtgt cgtagcgaac tcttatctgc catcaaactg gctttctgcg
    24541 ttggggctgt atgcctgggc gcgggtcgat gaatcttccg ataataattc attgcttaat
    24601 ccggcgaaga agtttacata ccaggctcca caaaacgttg atgatactta tgtcgtgttt
    24661 atcatcggtg aaaccacgcg ttgggaccat atgggtattt tcggctatga gcgtaatacc
    24721 acgccgaaac tggcccagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc
    24781 gcaaccaaac tctcactgcg ttgcatgttt gtacgtcagg ggggcgcgga agataatccg
    24841 cagcgcacat taaaagaaca gaacattttc gcggttctga agcagttagg attcagttct
    24901 gacctctacg ctatgcagag cgaaatgtgg ttctacagca acacgatggc agacaacatt
    24961 gcttatcgtg agcagattgg tgcggagcca cgtaatcgtg gcaagccggt agatgatatg
    25021 ttgctggtag acgaaatgca gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc
    25081 attctgcata ccaaaggttc gcattttaac tacacccagc gttatccgcg tagcttcgcg
    25141 cagtggaagc cggaatgtat tggtgttgat agcggctgta ccaaagcgca gatgatcaac
    25201 tcctatgaca actcggtgac ctatgtggat cactttatct ccagcgtgat tgatcaggtt
    25261 cgcgataaga aagcgattgt gttctacgca gctgaccacg gtgagtcaat taatgaacgc
    25321 gagcacctgc acggcacgcc gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg
    25381 atggtctgga tgtcagataa atatctggaa aatccggcca atgcgcaggc gtttgcgcag
    25441 ctgaaaaaag aagccgacat gaaagtgcca cgccgtcacg tagagctgta cgacaccatc
    25501 atgggttgtc ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgt
    25561 cacatcccgc agacaaaagt ggcagcggct aactaagcgg cctgctgact ttctcgccga
    25621 tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc
    25681 ccgcattcgg tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag
    25741 gttcgaatcc tctatcaccg accaaattcg aaaagcctgc tcaacgagca ggtttttttg
    25801 catttgtagc tcatgaggat gagaacctcc gggggcaggg aggctcgact cgacgtaggc
    25861 ctgataagac gcgccagcgt cgcatcaggc aacggcaccg cacttattgt cggatgcggc
    25921 gcgaccgcct tattcgacca acacaccaca cattttcctg ccaactttgc gccccatatc
    25981 tcactatcca ttaacggatt tgtgacagaa accattgtct ttttttatat ttgcttaaat
    26041 cttttttgcg ttacttatgg ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg
    26101 gtattaatcg ctcagatatc caccttttcc tcagattttt ttcattaaat gcataaaact
    26161 taatcaccaa atgttgctaa atattaagct gattgttctg gttatgttgg agtagcacaa
    26221 atttctctgc tgcaaatagc tgtttgaagt ttttttaatc tttgtttgtg gattctcacc
    26281 gttggtgtaa atcccggttg gctgtattga cgttttcaca ttctgttgac agattgtagg
    26341 tcacgagggg cattttatgg aggatccgca ctgttacact gatgttaatt agtacggcat
    26401 ccccacctca taacgttgac ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac
    26461 ccactgcaaa gggttaaaac aacaaacatc acaattggag cagaataatg cgtatttcct
    26521 tgaaaaagtc agggatgctg aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa
    26581 gtgttcaggc taaaactctg gtttattgct cagaaggatc tccggaaggg tttaacccgc
    26641 agctgtttac ctccggcacc acctatgacg cctcttccgt cccgctttat aaccgcctgg
    26701 ttgaatttaa aatcggcacc accgaagtga tcccgggcct cgctgaaaag tgggaagtca
    26761 gcgaagacgg taaaacctat accttccatc tgcgtaaagg tgtgaagtgg cacgacaata
    26821 aagaattcaa accgacgcgc gaaatgaacg ccgatgacgt ggtgttctcg ttcgatcgtc
    26881 agaaaaacgc gcaaaacccg taccataaag tttctggcgg cagctacgaa tacttcgaag
    26941 gtatgggctt gccggagctg atcagcgaag tgaaaaaggt ggacgataac accgttcagt
    27001 ttgtgctgac tcgaccggaa gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta
    27061 ttctgtcaaa agaatatgct gacgcgatga tgaaagccgg tacaccggaa aaactggacc
    27121 tcaacccaat cggaaccggt ccgttccagt tacagcagta ccaaaaagat tcccgtatcc
    27181 gctacaaagc gtttgatggc tactggggca ccaaaccgca gatcgatacg ctggtcttct
    27241 ctattacccc tgatgcttcc gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga
    27301 tgccgtaccc gaacccggca gacatcgctc gcatgaagca ggataaatcc atcaacctga
    27361 tggaaatgcc ggggctgaac gtcggctacc tctcgtataa cgtgcagaaa aaaccgctgg
    27421 atgacgtgaa agttcgccaa gcactaacct acgcggtgaa caaagacgcg atcatcaaag
    27481 cggtttatca gggcgcgggt gtatcagcga aaaacctgat cccgccaacc atgtggggct
    27541 ataacgacga cgttcaggat tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag
    27601 aagcgggtct ggaaaaaggt ttctccatcg acctgtgggc gatgccggta caacgtccgt
    27661 ataacccgaa cgctcgccgc atggcggaga tgattcaggc agactgggcg aaagtgggcg
    27721 tgcaggccaa aatcgtcacc tacgaatggg gtgagtacct caagcgtgcg aaagatggcg
    27781 aacatcagac ggtaatgatg ggctggactg gcgataacgg ggacccggat aacttcttcg
    27841 ccaccctgtt cagctgcgcc gcctctgaac aaggttctaa ctactcaaaa tggtgctaca
    27901 aaccgtttga agatctgatt caaccggcgc gtgctaccga cgaccataac aaacgcgttg
    27961 aactgtacaa gcaagcgcag gtggtgatgc acgatcaggc tccggcactg atcatcgctc
    28021 actccaccgt gtttgaaccg gtacgcaaag aagtcaaagg ctatgtggtt gatccattag
    28081 gcaaacatca cttcgaaaac gtctctatcg aataattaaa agccatacaa gactgatggc
    28141 aaaggcaaaa ctgcctgatg tgctccgctt atcaggccta cgaaaattct gcaatgtatt
    28201 gaatttgcac gattttgtag gccggataag gcgttaacgc cgcatccggc ataaacaaag
    28261 cgcactttgt caacaatctg tatacccggt ggcgctgtgc ctctgcgtgc agcgccatcc
    28321 ggcagcaata cttcattctc tgtccgacaa gggcgggaat tgatttgtga gcaatacaga
    28381 cacgcagttc caggctgcga gtcactatag agaatccggg ttatgttgca gtttattctc
    28441 cgacgtttgg gactcgtcat ccccacgttt atcggtatta cccttctcac atttgccttt
    28501 gtccacatga tcccgggcga tccggtgatg atcatggcag gcgaacgtgg gatctcccct
    28561 gagcgtcacg tgcagctgct ggctgaactc ggcttagata aaccgatgtg gcagcagtat
    28621 ctccattaca tttggggcgt aatgcacggc gatctaggca tttcaatgaa aagccgaatt
    28681 ccagtatggg aagagttcgt gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg
    28741 atgatttttg ctacggcagt cggtattccg gttggtgtgc tggctgcggt taaacgcggt
    28801 tccattttcg atcacactgc agttggcctg gcgctgaccg gctactcgat gccgatcttc
    28861 tggtggggca tgatgctgat catgctggtt tcggtgcact ggaacctgac gcccgtctcc
    28921 ggtcgcgtga gcgatatggt gttcctcgat gactccaatc cgttaaccgg ttttatgcta
    28981 atcgacaccg ccatctgggg tgaagacggc aactttatcg atgccgtcgc ccatatgatc
    29041 ttgcccgcca ttgtgctggg tactattccg ctagcggtca ttgtgcgtat gacacgctcc
    29101 tcgatgctgg aagtgctggg cgaggattac atccgcaccg cgcgcgccaa agggctaacc
    29161 cgcatgcggg tgattatcgt ccatgcgctg cgtaacgcga tgctgccggt agtgaccgtt
    29221 atcggcctcc aggtgggaac attgctggcg ggggcgattc tgaccgaaac catcttctcg
    29281 tggcccggtc tggggcgctg gttgattgac gcactgcaac gccgcgacta tccggtagtg
    29341 cagggcggcg tattgctggt ggcgacgatg attatcctcg tcaacttgct ggtcgatctg
    29401 ctgtacggcg tggtgaaccc gcgtattcgt cataagaagt aaggggacat catgtcacag
    29461 gttactgaaa ataaagtgat tagcgcaccg gtgccgatga ccccgttaca ggagttctgg
    29521 cactatttta aacgcaacaa aggcgcggtc gtcgggctgg tttacgtcgc catcgtgctg
    29581 ttcatcgcga tctttgccaa ctggattgcg ccttataacc cggcggatca gttccgcgat
    29641 gcgctgctcg ccccgccagc ctggcaggaa ggcggcagta tggcacactt gctgggcacc
    29701 gatgacgtcg gtcgtgatgt gctgtcgcgg ctgatgtacg gcgcgcgttt atcgctgctg
    29761 gttggctgtc tggtggtggt gttgtcgctg attatgggcg ttattctcgg cctgatcgcc
    29821 ggttacttcg gcggcctggt tgataacatt atcatgcgcg tggtcgatat catgctggcg
    29881 ctgccaagcc tgctgttggc actggtgctg gtggcgattt tcggcccgtc gattggtaac
    29941 gccgcgctgg cgctgacctt cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc
    30001 gtgctggtgg aggtgaatcg cgattacgtc accgcctctc gcgtggcagg tgccggggcg
    30061 atgcgccaga tgtttattaa catcttcccg aactgccttg cgccgctgat tgttcaggcg
    30121 tcgctcggtt tctctaacgc cattctcgat atggctgctc ttggtttcct cggcatgggg
    30181 gcacagccgc caacgcctga gtggggcact atgctctccg acgtgttgca gttcgcgcaa
    30241 agcgcctggt gggtcgtgac cttccctggt ctggcgatcc tgttgacggt gctggcattt
    30301 aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca aactaaagca gtaagaggtt
    30361 cgagatggcg ttattaaatg tagataaatt atcggtgcat ttcggcgacg aaagcgcacc
    30421 gttccgcgcc gtagaccgca tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt
    30481 gggtgagtcc ggctccggta agtcggtcag ttcactggcg attatggggc tgattgatta
    30541 tccgggccgc gtaatggcag aaaaactgga gtttaacggc caggatttgc agcgtatctc
    30601 agaaaaagag cgccgcaacc tggtgggtgc cgaagtggcg atgatcttcc aggacccgat
    30661 gaccagcctt aacccgtgct acaccgtggg tttccagatt atggaagcga ttaaagtgca
    30721 tcagggcggc aacaaaagta cccgccgtca gcgggcgatt gacctgctga atcaggtcgg
    30781 tattcccgat ccggcttcgc gtctggatgt ttacccgcat cagctttccg gcggcatgag
    30841 ccagcgcgtg atgatcgcca tggcgattgc ctgtcggcca aaactgctga ttgccgatga
    30901 accgaccacc gcgctggacg tgaccattca ggcgcaaatc atcgaactac tgctggagct
    30961 acagcagaaa gagaacatgg cgctggtgtt aattacccat gacctggcgc tggtggcgga
    31021 agctgcgcat aaaatcattg tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca
    31081 cgccatcttc catgcgccgc gtcacccgta tactcaggca ttgctgcgtg cgctgccaga
    31141 atttgctcag gacaaagaac gtctggcgtc gttgccaggt gttgttcccg gcaagtatga
    31201 ccgcccgaac ggctgcctgc ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc
    31261 tgaagaaccg gcgctgaata tgctcgctga tgggcgtcag tccaaatgcc attacccact
    31321 tgatgatgcc gggaggccca cactatgagt acgcaagagg ccacctcgca acaaccgctg
    31381 ttgcaggcta tcgacctgaa aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa
    31441 cgtctggtga aggcgctgga cggcgtttcg tttaaccttg aacgtggcaa aacgctggca
    31501 gtagtgggcg aatctggctg cggtaaatcg accctcggtc ggttgctgac gatgatcgaa
    31561 acgcccaccg gcggcgagct gtattatcag gggcaggatc tgctcaagca cgatccgcag
    31621 gcgcagaagc tgcgtcggca gaaaatccag atcgtcttcc agaatcccta cggttcgctg
    31681 aatccgcgta agaaagttgg gcaaattctc gaagagccgc tgcttattaa caccagctta
    31741 agcaaagaac agcgtcggga aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc
    31801 gagcactatg accgctatcc gcacatgttt tccggcggac aacgtcagcg tatcgctatt
    31861 gcccgtggtc tgatgctcga cccggatgtg gtgattgccg atgagccggt ttctgcgctg
    31921 gacgtctcag tgcgtgcgca ggtgctgaat ctgatgatgg atttgcagca ggagttgggg
    31981 ctttcttatg tctttatctc ccacgacctg tcggttgtgg agcacattgc tgatgaagtg
    32041 atggtgatgt acctaggccg ctgcgtggag aaaggaacga aggaccaaat cttcaataac
    32101 ccgcgccatc cgtacaccca ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat
    32161 cgtcgtgaac gcatcaaact caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt
    32221 tgcgccttca acgcccgctg tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag
    32281 ctaaaagact acggaggtca actggtagct tgttttgctg ttgatcagga tgaaaatccg
    32341 cagcgttaac tcacaaaacc ggggccatgc gctccggttt actcaatcag cttcaacagt
    32401 ggcgacaagc atagcaaaat gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt
    32461 ttttccagtt gtgcgacttt atagatcagg aaaaacggaa tgaggcacga cacaatgcca
    32521 tataacgggc ttcccaactg aaagaacacc agcactgata cacgaaacga aacccaaatc
    32581 gtcaacgtaa tgacgataaa agcgcagatc gccagagtca gcatgcgtga gttaattttc
    32641 ttggtatcaa taattcggct taacagattg agaataatgc ctttaatagc ctcgtggaaa
    32701 ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga tattcagcac cgtagaggtg
    32761 atatgaatga tatgtccagg gatcacctgc gcggccagtg ccagtgccga gatattttgt
    32821 tcaaaggcag aaacggcttc ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac
    32881 aggatcaccg cgatgagcgt gatataacta attcggtggg tacgcagcgc gagccgggtt
    32941 gccagtactt tatctgcttc ccgtttacga taggcaatat tcattgggtt aagtacctga
    33001 ataaataccg cagaaaagaa gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg
    33061 gaggcttgcg ggaaggcggt tatattggcg aaattccagt gcgggatcat cgcaaaaccg
    33121 aacacgacaa taatccctac tttgaccacc accattggcc cagaaatctt aaacagtaat
    33181 cgttcaccac cagacgcaat cgctatcagt acggcgaaaa tagcgacttt ataaagtaga
    33241 gattgtgaaa gatcggcatc ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa
    33301 accacggaga gagagtagat aaaaatcccg tggataatca tcaaaaagta gataaccccg
    33361 aggaaaattc cccagttctt ccccaggtaa tgactgataa tatcggtgta gtcattacag
    33421 gaatcgcttt cagaaagggt ttttaaataa atgtcctgca ctacccaggt gacaggataa
    33481 gcaatgatcg ctgcggtaat aaatacccaa attcccttca agccaatttg tactggcatc
    33541 agcacggttc cggcaccaat cgccatgcct atgcataata aaacccagcc gaagtcgtag
    33601 cgtgtaaacg gcaatttctg attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt
    33661 ccagaaagaa gtcggcctcg caaaaatgaa taaacccagt ttctcttgcg caacaggtgg
    33721 agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag
    33781 gaggcgcaat tctacaaaat tgcgcatttc actaccagtc tggagtcagc ccggatatga
    33841 actgcaacta ttgcaaataa agaaattaag ctggaaggca tcgcgcaatg aggaagattg
    33901 ccgggtgctg acagtaaaaa gca
//
LOCUS       DAJTNV010000038        33543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNV010000038 DAJTNV010000000
VERSION     DAJTNV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO199_001861"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO199_001861"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5535919.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO199_001862"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO199_001862"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5535920.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO199_001863"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO199_001863"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5535921.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO199_001864"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO199_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5535922.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO199_001865"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO199_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5535923.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO199_001866"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO199_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5535924.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO199_001867"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO199_001867"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5535925.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO199_001868"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO199_001868"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5535926.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO199_001869"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO199_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5535927.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO199_001870"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO199_001870"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5535928.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO199_001871"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO199_001871"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5535929.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO199_001872"
     CDS             8212..9075
                     /locus_tag="OO199_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5535930.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO199_001873"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO199_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5535931.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO199_001874"
     CDS             10411..11028
                     /locus_tag="OO199_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5535932.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO199_001875"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO199_001875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5535933.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO199_001876"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO199_001876"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5535934.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO199_001877"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO199_001877"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5535935.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO199_001878"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO199_001878"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5535936.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO199_001879"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO199_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5535937.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO199_001880"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO199_001880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5535938.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO199_001881"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO199_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5535939.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO199_001882"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO199_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5535940.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO199_001883"
     CDS             21859..23568
                     /locus_tag="OO199_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5535941.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO199_001884"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO199_001884"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5535942.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO199_001885"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO199_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO199_001886"
     tRNA            27625..27719
                     /locus_tag="OO199_001886"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="OO199_001887"
     CDS             28020..29204
                     /locus_tag="OO199_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5535943.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="OO199_001888"
     CDS             29616..30659
                     /locus_tag="OO199_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5535944.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OO199_001889"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OO199_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="OO199_001890"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="OO199_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="OO199_001891"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="OO199_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8403 a         8816 c         8267 g         8057 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact tgaaccgccc
    33541 cgg
//
LOCUS       DAJTNV010000039        33129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNV010000039 DAJTNV010000000
VERSION     DAJTNV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(2..94)
                     /locus_tag="OO199_001892"
     tRNA            complement(2..94)
                     /locus_tag="OO199_001892"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO199_001893"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO199_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5535945.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO199_001894"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO199_001894"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5535946.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO199_001895"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO199_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5535947.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO199_001896"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO199_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5535948.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO199_001897"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO199_001897"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5535949.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO199_001898"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO199_001898"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5535950.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO199_001899"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO199_001899"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO199_001900"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO199_001900"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5535951.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO199_001901"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO199_001901"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5535952.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO199_001902"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO199_001902"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5535953.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO199_001903"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO199_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO199_001904"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO199_001904"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5535954.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO199_001905"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO199_001905"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5535955.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO199_001906"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO199_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5535956.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO199_001907"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO199_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5535957.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO199_001908"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO199_001908"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5535958.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO199_001909"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO199_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5535959.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO199_001910"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO199_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5535960.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO199_001911"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO199_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5535961.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO199_001912"
     CDS             complement(20900..21019)
                     /locus_tag="OO199_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5535962.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO199_001913"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO199_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5535963.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO199_001914"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO199_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5535964.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO199_001915"
     CDS             complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="OO199_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5535965.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO199_001916"
     CDS             complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="OO199_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5535966.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHRCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO199_001917"
     CDS             complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="OO199_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5535967.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26927..27277
                     /gene="hypA"
                     /locus_tag="OO199_001918"
     CDS             26927..27277
                     /gene="hypA"
                     /locus_tag="OO199_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5535968.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27281..28153
                     /gene="hypB"
                     /locus_tag="OO199_001919"
     CDS             27281..28153
                     /gene="hypB"
                     /locus_tag="OO199_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5535969.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28144..28416
                     /gene="hypC"
                     /locus_tag="OO199_001920"
     CDS             28144..28416
                     /gene="hypC"
                     /locus_tag="OO199_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5535970.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28416..29537
                     /gene="hypD"
                     /locus_tag="OO199_001921"
     CDS             28416..29537
                     /gene="hypD"
                     /locus_tag="OO199_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5535971.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29534..30544
                     /gene="hypE"
                     /locus_tag="OO199_001922"
     CDS             29534..30544
                     /gene="hypE"
                     /locus_tag="OO199_001922"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5535972.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30618..32696
                     /gene="flhA"
                     /locus_tag="OO199_001923"
     CDS             30618..32696
                     /gene="flhA"
                     /locus_tag="OO199_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5535973.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32733..33086)
                     /locus_tag="OO199_001924"
     CDS             complement(32733..33086)
                     /locus_tag="OO199_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5535974.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7806 a         9232 c         8622 g         7469 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcagg ataatgccga tattttccag ggtgtggtaa
    24661 gccagcaggc gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg
    24721 atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt
    24781 gacagggtta acatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca
    24841 gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatccca
    24901 gccagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt
    24961 tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag
    25021 caagcaatca ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct
    25081 ttgctgttgc tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac
    25141 atgccgaggt tgctggcgat gacggcgcag acggcggcag ccatcaacat attgatctgc
    25201 aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac
    25261 agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg
    25321 tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc
    25381 gcggctgccg tatacagact accaaccgcg ccgccaattc cggctatcca gccacttagc
    25441 gctttttgaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    25501 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    25561 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    25621 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    25681 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    25741 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    25801 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    25861 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    25921 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gcggtgacag
    25981 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    26041 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    26101 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    26161 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    26221 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    26281 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    26341 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    26401 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    26461 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    26521 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    26581 ggcgtgatac aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    26641 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    26701 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    26761 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    26821 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    26881 tggcgcaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    26941 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    27001 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    27061 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    27121 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    27181 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    27241 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    27301 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    27361 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    27421 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    27481 tcacacaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    27541 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    27601 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    27661 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    27721 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    27781 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    27841 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    27901 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    27961 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    28021 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    28081 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    28141 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    28201 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    28261 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    28321 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    28381 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    28441 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    28501 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    28561 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    28621 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    28681 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    28741 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    28801 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    28861 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    28921 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    28981 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    29041 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    29101 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    29161 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    29221 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    29281 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    29341 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    29401 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    29461 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    29521 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    29581 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    29641 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    29701 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    29761 gatttgcggc accgcgaatg acgttgcggg cagtggcgct attccgcgct atctctcctg
    29821 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    29881 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    29941 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    30001 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    30061 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    30121 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    30181 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    30241 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    30301 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    30361 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    30421 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    30481 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    30541 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    30601 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    30661 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    30721 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    30781 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    30841 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    30901 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    30961 ccaaatttgg tcactattgc ctgatgccac tggcggcggg agggcatatt tttggtggct
    31021 gtgaatttat tcgttatgac gagcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    31081 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    31141 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    31201 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    31261 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    31321 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    31381 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    31441 atctctacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    31501 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    31561 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    31621 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    31681 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    31741 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    31801 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggctcgggta
    31861 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    31921 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    31981 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
    32041 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    32101 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    32161 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    32221 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    32281 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    32341 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    32401 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca
    32461 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    32521 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    32581 aaaccaacag cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    32641 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    32701 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    32761 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    32821 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    32881 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    32941 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    33001 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    33061 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta
    33121 catcttaat
//
LOCUS       DAJTNV010000040        32431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTNV010000040 DAJTNV010000000
VERSION     DAJTNV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..957)
                     /locus_tag="OO199_001925"
     CDS             complement(<1..957)
                     /locus_tag="OO199_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5535975.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1203..1829
                     /gene="gstB"
                     /locus_tag="OO199_001926"
     CDS             1203..1829
                     /gene="gstB"
                     /locus_tag="OO199_001926"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5535976.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OO199_001927"
                     /pseudo
     CDS             complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OO199_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OO199_001928"
     CDS             complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OO199_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5535977.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3647..4972
                     /gene="rimO"
                     /locus_tag="OO199_001929"
     CDS             3647..4972
                     /gene="rimO"
                     /locus_tag="OO199_001929"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5535978.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5019..6347)
                     /locus_tag="OO199_001930"
     CDS             complement(5019..6347)
                     /locus_tag="OO199_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5535979.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6355..8703)
                     /locus_tag="OO199_001931"
     CDS             complement(6355..8703)
                     /locus_tag="OO199_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5535980.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8881..9792)
                     /gene="gsiD"
                     /locus_tag="OO199_001932"
     CDS             complement(8881..9792)
                     /gene="gsiD"
                     /locus_tag="OO199_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5535981.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFISVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9795..10715)
                     /gene="gsiC"
                     /locus_tag="OO199_001933"
     CDS             complement(9795..10715)
                     /gene="gsiC"
                     /locus_tag="OO199_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5535982.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10733..12271)
                     /gene="gsiB"
                     /locus_tag="OO199_001934"
     CDS             complement(10733..12271)
                     /gene="gsiB"
                     /locus_tag="OO199_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5535983.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12291..14162)
                     /gene="gsiA"
                     /locus_tag="OO199_001935"
     CDS             complement(12291..14162)
                     /gene="gsiA"
                     /locus_tag="OO199_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5535984.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14149..15114)
                     /gene="iaaA"
                     /locus_tag="OO199_001936"
     CDS             complement(14149..15114)
                     /gene="iaaA"
                     /locus_tag="OO199_001936"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5535985.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15318..16553
                     /gene="moeA"
                     /locus_tag="OO199_001937"
     CDS             15318..16553
                     /gene="moeA"
                     /locus_tag="OO199_001937"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5535986.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16553..17302
                     /gene="moeB"
                     /locus_tag="OO199_001938"
     CDS             16553..17302
                     /gene="moeB"
                     /locus_tag="OO199_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5535987.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17481..18143)
                     /gene="fsa"
                     /locus_tag="OO199_001939"
     CDS             complement(17481..18143)
                     /gene="fsa"
                     /locus_tag="OO199_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5535988.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18274..19173
                     /locus_tag="OO199_001940"
     CDS             18274..19173
                     /locus_tag="OO199_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5535989.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19179..21611
                     /locus_tag="OO199_001941"
     CDS             19179..21611
                     /locus_tag="OO199_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5535990.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21757..22572
                     /gene="ybiV"
                     /locus_tag="OO199_001942"
     CDS             21757..22572
                     /gene="ybiV"
                     /locus_tag="OO199_001942"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5535991.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22724..23989
                     /locus_tag="OO199_001943"
     CDS             22724..23989
                     /locus_tag="OO199_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5535992.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24230..25822)
                     /locus_tag="OO199_001944"
     CDS             complement(24230..25822)
                     /locus_tag="OO199_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5535993.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26041..26961
                     /gene="ldtB"
                     /locus_tag="OO199_001945"
     CDS             26041..26961
                     /gene="ldtB"
                     /locus_tag="OO199_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5535994.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27019..28137)
                     /locus_tag="OO199_001946"
     CDS             complement(27019..28137)
                     /locus_tag="OO199_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5535995.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28134..28601)
                     /gene="mntR"
                     /locus_tag="OO199_001947"
     CDS             complement(28134..28601)
                     /gene="mntR"
                     /locus_tag="OO199_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5535996.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28787..28915
                     /gene="mntS"
                     /locus_tag="OO199_001948"
     CDS             28787..28915
                     /gene="mntS"
                     /locus_tag="OO199_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5535997.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29187..30770
                     /gene="opgE"
                     /locus_tag="OO199_001949"
     CDS             29187..30770
                     /gene="opgE"
                     /locus_tag="OO199_001949"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5535998.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30818..31333)
                     /gene="ompX"
                     /locus_tag="OO199_001950"
     CDS             complement(30818..31333)
                     /gene="ompX"
                     /locus_tag="OO199_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5535999.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31386..31451)
                     /locus_tag="OO199_001951"
     CDS             complement(31386..31451)
                     /locus_tag="OO199_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536000.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31686..>32431
                     /gene="rhtA"
                     /locus_tag="OO199_001952"
     CDS             31686..>32431
                     /gene="rhtA"
                     /locus_tag="OO199_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5536001.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALA"
BASE COUNT         8109 a         8649 c         7846 g         7827 t
ORIGIN      
        1 ggagcccgct tcgcctgccc cgagattcac ttcgatctta tcaccaaacc agacgcgctg
       61 agtcacaaag gtggcatcag gtttaattgg cgtcacagtt tcaaagaagc ggaaacccca
      121 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
      181 ggagattaaa cgcatatcac cctgggtagc cgaagcaacc agattatatc ccgcacctgc
      241 cgtggttcct gtcttcatgc catcaacatt cagattactg ctccacagca gacggttacg
      301 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
      361 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
      421 ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt
      481 ggcataacca ttcatcagac caataaagga ctcctggctc ccggcaacgt aatctgccag
      541 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
      601 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
      661 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
      721 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcaggat ccagtttctc
      781 atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
      841 cgcatccacg ctcggcgctt caacggtttg ttcggccgcg aatgccgttg gggcaaaaag
      901 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
      961 tgccacccga gtatccattc aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagctccc aataaataat ttttgctgcc actgggggaa
     2041 tttatcgctg ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac
     2101 aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata
     2161 gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat
     2221 ttcatcacca ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat
     2281 cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc
     2341 ctttataaaa ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc
     2401 ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa
     2461 aagataacct ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt
     2521 tggcatctgg cggaagacgg tgcggaagtc ggtcactttt gagagatcat cacttaagcg
     2581 gccataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca
     2641 gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc
     2701 gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca
     2761 gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc
     2821 cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc
     2881 cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat
     2941 aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt
     3001 tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc
     3061 tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt
     3121 tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa
     3181 catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga
     3241 tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag
     3301 ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc
     3361 acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt
     3421 ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg
     3481 ctattttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta acaaaattta
     3541 catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt
     3601 agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac
     3661 tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga
     3721 gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc
     3781 gaacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga
     3841 agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc
     3901 aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag
     3961 ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt
     4021 cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa
     4081 aatttcggaa ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga
     4141 cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg
     4201 cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca
     4261 tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca
     4321 gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga
     4381 cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca
     4441 gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact
     4501 ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat
     4561 tgtcggcttc cctggcgaga cggaagaaga tttccagatg ctactcgact tcctgaaaga
     4621 agcacgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa
     4681 tgccctgcct gaccaggttc cggaagaagt gaaagaagaa cgctggaacc gtttcatgca
     4741 gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt
     4801 gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga
     4861 aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg
     4921 tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca
     4981 ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgtgcc
     5041 ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg
     5101 gtagtacatt catttgaatg cgctacaccg atactggtgc ttaaattaat gcttcccacc
     5161 tggaaatgaa cgatttcttt tagtatcgat agcaactctt ccagcgtgcc gccagtataa
     5221 atcaccgcaa attcgtcacc tccgaagcgg tagactttac ctttatctac cgggatctgt
     5281 gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg
     5341 tcgttaatgt ttttgaattt atcgacgtca aacagacaca aataaccgcc tttttccggc
     5401 agcgctttta aatcttcatc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg
     5461 aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg
     5521 ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga
     5581 ttaatcactc tgcgtaccag atagaggcaa ctggcaccca atatcactgc caccaatgcg
     5641 gcaaagaaaa taatcaatgt acaaacccgc aaataaacat cttttttata cgcaaatacg
     5701 tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg
     5761 tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc
     5821 aggttctcgc tgtccatcac cagcgatttt gacagtaaag aaaaacgctc cagaccaaac
     5881 aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg
     5941 tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct
     6001 gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa
     6061 ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca
     6121 accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc
     6181 cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta
     6241 tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata
     6301 aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc
     6361 ttttactaat tttccacttt tatcccaagc ggagaatggc atgggtttct ggtacagata
     6421 cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac
     6481 gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat
     6541 ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg
     6601 attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat
     6661 ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc
     6721 gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat
     6781 gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat
     6841 atgcagtaag aaatcatcct tcagcgacgc acgttgacta aaatgtctga attcacccat
     6901 tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt
     6961 aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc
     7021 cgcgctcaga atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc
     7081 gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt
     7141 gagtttcgcg tcatcacaga aaataaactc ggagaacata tggtcacgga tactggaaat
     7201 cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat
     7261 ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat
     7321 cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc
     7381 attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt
     7441 cctgacgatt ttattgcgtt catcaacacc aaacaccgcc tccacggctt ccagaccaca
     7501 cagtttgatg atccccacag ccagatactg attattgttg gactcaataa tcgccatatt
     7561 tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc
     7621 aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc
     7681 gccttcgcga attttcagag cctcgatctg atccgttgag tcgggcaaaa acgccagcgt
     7741 attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata
     7801 taccaccagc aacacgataa taatgaacgc aaacggtaga taaaaaagtt tatctgatgc
     7861 gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac
     7921 tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc
     7981 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc
     8041 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
     8101 ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt
     8161 gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg
     8221 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
     8281 atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
     8341 ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag
     8401 taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg
     8461 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
     8521 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
     8581 ttttaccgcg cgttccgata ccagttgata gttaatcatg aaaaaaagac caataaatga
     8641 cagtgctgcc aataaaaata aaattatcag ccttatcttt atcttttcgt ataaactcag
     8701 catgttaata catttaacaa aataactatc tgaacgctgt gccgctgcaa aaatagtccc
     8761 ttgctgcggc tttttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac
     8821 ttgcttaggg caataatttc ctgtgaggtg attacccttt caagcaatat tcaaacgtaa
     8881 ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc
     8941 cagtacggtc agaaaaatag ccagggccgg aaaaacagcg acatgcggcg cgataaccat
     9001 atccgctcgt gcctcattga gcattgctcc ccactctggt gtcggcggct gcgcaccgag
     9061 gccgagaaat gacaggctgg cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa
     9121 caccacgata gaagagacgg tcccaggcag gatatgacgc aataaaatgg tcatatcgct
     9181 ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc
     9241 gcgcaccagg cgggcaaacg cggggatgga aaaaatggcg actgcgataa tcacgttagc
     9301 aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa
     9361 cagcacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccaa
     9421 caagcccagc aacgtcccta tcgccacact gataaacacg gcaaacacgc ccgccgccag
     9481 cgagatttgc gcaccaacca ggacacggct gaaaatgtcg cgccccagtg aatcgacgcc
     9541 aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc
     9601 gtcataggga gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa taacgaataa
     9661 tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt
     9721 acgtacctgg tcaggtttga ccagtggcat ggcgtttaac accgcctgac gtcgccagtt
     9781 aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc
     9841 accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc
     9901 gggtaatcgc gcatttctac agagtcaacg agtaagcgtc caagtccggg ccagttgaaa
     9961 actttctcca ccacgataga accaccgagc aaaaagccaa actgtaagcc catcatggtc
    10021 actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt ttcgctcacc
    10081 cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa tgacgcacgg
    10141 gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg taaaatgtag
    10201 tgctgccaac tgtctgctcc cacagtaggc agccagccca gttcaacgga gaatacctga
    10261 attaaaaaca tccccagcgc aaatgccgga aacgagatcc ccgacaccgc aatggtcata
    10321 ctcaatcgat ccggccaacg attacgccag acggcggcga taattcccgc cgccatacca
    10381 aatatcaccg cccagaccat actggttatg gtcagccaca gcgttggcat aaagcggctg
    10441 gcaatctcat cggcaaccgg acgacgcgac accatcgaca ggccaaaatc cccctgcaca
    10501 gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga
    10561 cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caatcaatcg cgccggatcg
    10621 ccgggcagca tgtggacaaa taaaaacacc agcaccgaga cgataaacag cgtcggaatc
    10681 aaccccagta atcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca
    10741 aatccgcgtc ttcaaagctg aagccggtat ctggcatgat ccaaaaaccg gtcaggtttt
    10801 tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgat tcttgccaga
    10861 tgatatcctg cgccgcctta tataagcggg tcttttccgc cggatcattc gttttcagtg
    10921 cctgagccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg
    10981 gtggccagtt ttgcgaggca aacagcggcg atagtgccca gtcggcttcg ccggttgaag
    11041 ccgaccagcc agtgtagaac atccgcacgc cactctcttt ttgcccttta ccttcaactt
    11101 ctgccgcccg ctgtccggca tccatcgcgg tcacctgggc tttaatcccg acctgcgcta
    11161 actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca
    11221 gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg
    11281 gatcgtaagg ccacggttta taactttgcg cgtaggcgat acttggcggt accacaccag
    11341 tagctggcgt tgcatagccc gcaaaggcaa ctttcaccag cgccggacgg ttaatggcgt
    11401 aattcagcgc ctcacggacc ttcgggttat cgaacggctt ttgcgtcacg ttcatactga
    11461 tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca
    11521 gtgtggcctg ctcgtaggga atggggaaag caaactgcgc ttcaccggtt tgcagcattg
    11581 ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc
    11641 ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat
    11701 ccagttcata cggtccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca
    11761 gcgctgtcgg tgaaatcatc gcggtcgccg gatgggcaag aatattaata aacgctgaga
    11821 acggctgttt gagggtaatc tttaccgttg tcggatcgat cgcctccgtt ttggcaatat
    11881 tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg
    11941 cttttaccgc cactgcgttg aaatcggtgc catcctggaa tttaattcct tcccgcaatt
    12001 tcacggtgta agtaatgccg tcatcggaaa cggtataact ctccgccagc acgtttttca
    12061 gtttcatctc tttatccaga ccgaacagcc cctggtaaaa cgatttcgct acggcctgag
    12121 ataacgtgtc attcgcgtca tacggatcga gcgtggtgaa attcgatcct accgccacca
    12181 ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca
    12241 ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc
    12301 atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac
    12361 gagacggctg ccacctcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc
    12421 agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta
    12481 cgcgtataag gatgctgcgg gttttcgaag accgcgcacc gtgaaccaat ttcaacaatt
    12541 tgcccgagat acatcaccgc cacacgatga ctaatccgct ctaccacagc catatcgtgg
    12601 gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc
    12661 tgcccgcgaa tagaaacatc cagcgccgaa acggcttcgt cggcaatgat cacttttgga
    12721 ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatgc
    12781 gggtaacgcc aggcatgttc aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt
    12841 gcagccgcgt ctttacctgg caataaaccg tgtacacgca gcggttcgat aatcgaatca
    12901 ccgatggtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc
    12961 cggcgcaatg cctgaagttt gccaggtgac aaggtatcga ttcgctgacc gttaaagata
    13021 atttcgccgc cctgcgattc gaccaggcgc agcaacgccc gcccggtagt ggatttaccg
    13081 ctgccagact cacccaccag cgatagcgtt tcgccaggcc agagatcaaa actgactttc
    13141 tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg
    13201 gtgacaagat tacgcacccg taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg
    13261 gggggttcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct
    13321 aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt
    13381 tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc
    13441 accagtaccc gatcggcaat ctctgccacc acgcccatat cgtgagtgat aaagataacg
    13501 cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg
    13561 acatccagcg cggtggttgg ctcatcggca atcagcaccg ccgggcggca tgacagcgcc
    13621 atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcgaata acgtgaaaga
    13681 atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct
    13741 tcttcacgac tggcgttctg atgcagacga attgattcgg caatctgttc acccacagta
    13801 aatactgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcgc
    13861 acatggcgca tttgtgcagc gctctgctcg ctaagttcaa tcacatcacg actgcgccgc
    13921 cgcaacagca ttttatcgca ctgtactaaa ccgcccgcct gttccaacag acgcatcaat
    13981 gccagcgcag tcactgactt accggagccg gattcgccta caattgccag cgtctcaccg
    14041 cgttgcagac taaaagagag attgcggacc gcagctattt tctgctggtc ctgcataaag
    14101 gcaatattaa gattttcaac cgccagcaca ttaccggcat caagttcatc actgtgtggc
    14161 aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca
    14221 ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc
    14281 gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc gctcgcaggc
    14341 ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atgtcatatg ccgccagcgc
    14401 gcggatgaag acttcgcctg tgccggtaca agaaaccgcc acactggcgt tattggcgta
    14461 gcatccggca cccactaagg gactatcgcc aactcgtccg ggtaatttat tggtcattcc
    14521 gcctgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg cccccacggt
    14581 gcccattttc tgtttttcat ccagtggcgc accgctatgg tcgaggactg ttgccccttc
    14641 ctcgcgcgct gccagtagtt gttcataacg caaaggcgtg gagaaaatct ccggtgagac
    14701 gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg
    14761 cgggctttgc tccatcacca gccgggcggc aagaaccgga ttacgcagat gactaacgcc
    14821 cgccaccgca ccggctttca gggtgttacc atccatcaca caggcgtcca gttcatgggt
    14881 ttcatcacgc gtaaagacag cgccaattcc agcgttaaac agtggacact cttccagcag
    14941 acgcaccgct tccgtcacca catccagcgc actttcgccg gcttccagca ttttctgccc
    15001 ggtttcaaca atggcagaca acgcctcgat gtagcgtaat tcctgttgca gactcatctg
    15061 cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt
    15121 cgtccattaa gggtggctcg ccacttttgc tataagtatc agaattttta tgaattactc
    15181 attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggagc caacgctacg
    15241 cccgaggtgg catacagtct atggcgtttt tctgacataa taggcaaatt cgattttgcc
    15301 tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta
    15361 atgagatgct ttctcgcgtt accccactga ccacccagga aacgctgcca ctggttcagt
    15421 gttttggtcg tattctggcg agcgatgtcg tttcgccact ggatgtcccg gggtttgata
    15481 actccgctat ggacggctac gcggtgcgtt tagccgatat tgcctccggg caaccgctgc
    15541 ccgttgccgg taaatccttt gccggtcagc cataccatgg tgaatggcct gcgggtacct
    15601 gcattcgtat tatgaccggt gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg
    15661 agcagactga acaaacggac aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa
    15721 atattcgccg tcgcggtgaa gatatctctg caggtgcggt tgttttcccg gcgggaactc
    15781 gcctgactac cgcagagctg ccagtaattg cttcactggg gattgccgaa gttccggtga
    15841 ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg cccggtcagc
    15901 cgctgggcga cggccaaatc tacgacacca accgtctcgc cgtacacctg atgttagaac
    15961 agctgggttg cgaggtaatc aacttaggga ttatccgcga cgatccacat gccctgcgcg
    16021 ccgcatttat tgaagccgac agccaggcgg atgtagtgat cagttccggc ggtgtttcag
    16081 tgggtgaagc ggattacacc aaaatgattc ttgaagagct gggggagatc gccttctgga
    16141 agctggcgat taaaccaggt aaaccgttcg cgttcggtaa actcagcaat agctggttct
    16201 gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt
    16261 tgctggcaaa actaagcggc aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca
    16321 cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc gtgctgcaac
    16381 gcaacgccga cggtgaactt gaagtgacga ccaccggaca tcagggttca catatattta
    16441 gctcctttag tctcggcaac tgctttatcg tgctggaacg cgatcgcggc aatgtggacg
    16501 tgggcgaatg ggtggaagta gaaccgttta acgcgttgtt cggaggcctg taatggcgga
    16561 actcagcgat caggagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt
    16621 tgacggtcag gaggcgctaa aagattctcg tgtgctggta gtcggcctcg gtggactcgg
    16681 ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt
    16741 cgacacggtt tcgctctcga atctgcaacg ccagacactg cacagtgatg ccacggtcgg
    16801 gcaaccgaag gtggaatccg cccgtgacgc cctgacgcgg atcaacccac atatcgcgat
    16861 tacgccagtc aatgcactgc tagatgacgc agaacttgca gcattgattg ctgaacacga
    16921 tctggtgctc gactgtacgg ataacgttgc ggtacgtaat caactgaacg caggctgttt
    16981 tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaagggc aaatcacggt
    17041 cttcacttat caggacggcg aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa
    17101 cgcgttaacc tgcgtagaag caggcgtgat ggcaccgttg ataggcgtaa ttggttcgtt
    17161 gcaggcaatg gaagcgatca aactgctggc aggttatggc aaaccggcca gcgggaaaat
    17221 cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg
    17281 gtgtgaggtg tgcgggcagt aattgccacc tccaagcctg ataagacgcg acaagcgtcg
    17341 catcaggcat ttgctccaga cgccagatgc ggcgtgaacg ccttatctgg cctacggttc
    17401 gggcacagat ttccaagcgc ggcaggcatc ggtgcactcc gccacgtcat cagcaattta
    17461 tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa acgctccctg ccagtcctgc
    17521 tcaaacttcg tcacagcggc ttccaccgcc ggatagctaa tcatctgttg tgccacatcc
    17581 agtggcagag taattgattc acatccggcc agtaagcagt ccagcgcctg acgcggggtt
    17641 ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag
    17701 tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa tacgattaac gtaaggcgca
    17761 acatattccg cacctgccag cgccgacagc agcccttgtg ccgcgccata taccgcggtt
    17821 cccagcgtcg gaatcccttc cgcttttaac atcttaatcg ctgccagccc ctcggctgtc
    17881 accggaactt tcaccacgat atccgcaata atagaacgca gcttaagcgc gtcattcacc
    17941 atcccttcag cagtggtagc cattacctgg gcaaacagac gcccctgacc gcccatcgct
    18001 tcatgaagtt gcggaagcac aacatccagc ggttttttac ccgcggcgat aatgcttggg
    18061 ttagtggtca cacccgccag cggaaaaata cgtgacagcg ccttcaccgc aacaacgtct
    18121 gaagtatcca gatacagttc cataaccatc ctcaaaatgt actttttgtc tgaatgtaga
    18181 ctatcacggg aaaactctct gaacagttga cgcacatcaa tctgactttc attcgaaagt
    18241 attttaatgt ttgaacgaaa cagagaggca actatgattt tcaatattca gcgctactcg
    18301 acccatgatg gccccggtat ccgcacggtc gtatttctta aaggctgttc gctgggctgc
    18361 cgctggtgtc agaacccgga aagccgcgcc cgtacgcagg atctgctgta tgacgcacga
    18421 ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc cggaagtgat tgagcgcgcg
    18481 ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg agcatctgac ggcgttaacc
    18541 gactgctgtc cgacacaggc gttaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc
    18601 atggcgaccg ttctgcgcga taaaccgttt tacgatcgca gcggcggcgg cttaacgctt
    18661 tcgggtggtg aacccttcat gcagccggaa atggcgacgg cgctgctgca agcctgccac
    18721 gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc
    18781 ccttctctgc cctatatcga tctgtttctt gccgatttaa aacacgttgc cgacgcgccg
    18841 tttaaacagt ggaccgacgg taacgccgcc agggtgctgg ataacctgaa aaaactcgcc
    18901 gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc agggctttaa tgccgacgaa
    18961 acctctgtaa aagccattac cgattttgcc gccgacgagc tgcacgttgg cgaaattcat
    19021 tttctgccct accacacgct gggcatcaac aaatatcact tacttaatct gccctatgac
    19081 gccccggaaa aaccgcttga tgcgccagag ctgctcgatt ttgcccagca gtatgcctgc
    19141 cagaaagggt taaccgcgac cttacgagga taacaaccat gaccacactg aaactggaca
    19201 cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt gcatattgtg aagccgccag
    19261 tctgtaccga gcgcgcgcag cactataccg agatgtatca acaacatctc gataagccga
    19321 tcccggtacg tcgcgcgctg gcactggcgc atcacctggc gaatcgcacc atctggatca
    19381 aacacgatga gttgatcatt ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc
    19441 cggaatatac tgtctcgtgg atcgaaaaag agattgatga tctggcagat cgtcccggtg
    19501 ctggctttgc ggtgagcgaa gagaacaaac gtgttctgca tgaagtgtgc ccatggtggc
    19561 gcggtcagac ggtacaggat cgctgctatg gcatgtttac cgatgagcaa aaaggtctgc
    19621 tggcgaccgg catcattaaa gcggaaggca atatgacctc cggcgatgcg cacctggcgg
    19681 tcaatttccc gctgctgctg gaaaaagggc ttgatggtct gcgtgagaaa gtagcggaac
    19741 gtcgctcgcg catcaatctg acggtgctgg aagatttgca cggcgaacag ttcctgaaag
    19801 cgattgatat cgtgctggtg gcagtcagtg aacacattga acgtttcgct gccctggcgc
    19861 gtgcaatggc cgcgaccgaa acccgcgaaa gccgtcgcga tgaactgctg gcgatggcag
    19921 aaaactgcga tcttatcgcc caccagccgc cgcagacttt ctggcaggcg ctgcaactgt
    19981 gttacttcat ccagttgatt ttgcagattg aatctaacgg tcactcggtg tcgtttggtc
    20041 gtatggatca gtatctctac ccgtactatc gccgcgacgt tgaactgaac cagacgctgg
    20101 atcgcgaaca cgccatcgaa ttgctgcata gctgctggtt gaaattgctg gaagtgaaca
    20161 aaatccgctc cggctcccac tcaaaagcct ctgcgggaag tccgctgtat cagaacgtca
    20221 ccattggcgg acaaaatctg gttgatggtc aaccaatgga cgcggtgaat ccactctctt
    20281 acgcgatcct cgaatcctgc ggtcgcctgc gttccactca gcctaacctc agcgtgcgtt
    20341 accatgcagg aatgagtaac gatttcctcg acgcctgcgt acatgtgatc cgctgcggct
    20401 tcgggatgcc ggcattcaac aacgacgaaa tcgtgatccc ggaatttatt aaactcggta
    20461 ttgaaccgca ggacgcttat gactacgcag cgattggttg tatagaaacc gccgtcggtg
    20521 gcaaatgggg ctatcgctgt accggcatga gctttatcaa cttcgcccgc gtgatgctgg
    20581 cggcgctgga aggcgggcgt gatgccacca gcggcaaagt gttcctgcca caagaaaaag
    20641 cgttgtcggc aggtaacttc aacaacttcg atgaagtgat ggacgcgtgg gatacgcaaa
    20701 tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt cgtcgacacc atgctggaag
    20761 agaacgtgca cgatattctc tgctcggcgc tggtggatga ctgtattgag cgagcgaaaa
    20821 gtatcaagca aggcggcgcg aaatatgact gggtttctgg cctgcaggtc ggtattgcca
    20881 acctcggcaa cagcctggcg gcagtgaaga aactggtgtt tgaacagggc gcgattggtc
    20941 agcaacagct ggctgccgca ctggcggatg acttcgatgg cctgactcat gagcaactgc
    21001 gtcagcggct gattaacggt gcgccgaagt acggcaacga cgatgatacc gtcgatacgc
    21061 tgctggctcg cgcttatcag acctatatcg acgaactgaa acagtaccat aacccacgct
    21121 acggtcgtgg tccggttggc ggcaactatt acgcgggtac gtcgtctatc tccgctaacg
    21181 taccgtttgg cgcgcaaact atggcaacgc cggacgggcg taaagcccac accccgctgg
    21241 cagaaggcgc tagtccggcc tctggtactg accatctcgg ccctactgcg gtcattggct
    21301 cagtgggtaa actgcctacg gcagcgattc tcggcggcgt gttgctcaac cagaaactga
    21361 atccggcaac gctggagaac gaatctgaca agcagaaact gatgatcctg ctgcgtacct
    21421 tctttgaagt gcataaaggc tggcatattc agtacaacat cgtttcccgc gaaacgctgc
    21481 tggaggcgaa aaaacatccg gatcagtacc gcgatctggt agtgcgtgtc gcgggttatt
    21541 ccgccttctt caccgcgctc tctccagacg ctcaggacga tatcatcgcc cgtactgaac
    21601 atatgctgta atctcttcgg cccggctgcg gcgtcgccgg gctaaatcat tcacatcatc
    21661 aatttcatcc ttactttcat tcgaaatata atttgtgctc tgcgtcacat tgttattaca
    21721 ctgttacggt ttcattttcg agccaggagc acacctatga gcgtaaaagt tatcgtcaca
    21781 gatatggacg gtacttttct taacgacgcc aaaacgtaca accaaccacg ttttatggcg
    21841 caatatcagg aactgaaaaa gcgcggcatt aagttcgttg tcgccagcgg taatcagtat
    21901 taccagctta tttcgttctt tcctgagcta aaggatgaga tctcttttgt cgcggaaaac
    21961 ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg gcgaactgac ccgacatgaa
    22021 tcgcggattg ttattggcga gttgctaaaa gataagcaac tcaattttgt cgcctgcggt
    22081 ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat ttgtcgcact gatggcaaaa
    22141 cactaccatc gcctgaaacc tgtaaaagat tatcaggaga ttgacgacgt actgttcaag
    22201 ttttcgctca acctgccgga tgaacaaatc ccgttagtga tcgacaaact acacatagcg
    22261 ctggatggca ttatgaaacc cgtcaccagt ggttttggct ttatcgacct gattattccc
    22321 ggtctacata aagcaaacgg tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa
    22381 aatgtggtag cgattggcga cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat
    22441 tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat
    22501 gataataatc atgaaggcgc gctgaatgtg attcaggctg tgctggataa cacatcccct
    22561 tttaacagct gacctctcta ccggagccat gcgctccggt tttcccttct ttttacatcc
    22621 tgtacatcac atcaaatcat taaacttgca ttaacttatt tataacttgt attgtcacat
    22681 gcaaattagc atagtttcga atgaaagtta atgcgaggtg gttatggctt ctacttttac
    22741 cagcgacaca ttgcctgccg atcacaaagc agctatccgt cagatgaagc acgcgctgcg
    22801 ggcgcagctt ggcgatgtcc agcagatctt taatcagcta agcgatgaca ttgccacgcg
    22861 agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc gtctggccgg tgctgtctta
    22921 tgccgatatc aaagcaggtc atgttactgc agagcagcgc gaacagatta aacgtcgcgg
    22981 ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg ctaggctggg atcagtcgat
    23041 gctggactat ctgaaccgca accgctttga cgaggtctac aaaggccccg gcgataattt
    23101 cttcgggacg ctcagcgctt cacgtcccga gatttacccc atctactggt cgcaggcgca
    23161 aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag tcgtttctca atcgtctgtg
    23221 gacatttgaa agtgatggaa agcaatggtt taacccggat gtgagcgtca tctaccctga
    23281 ccgtatccgc cgccttccgc ccggaacgac ctccaaaggt cttggagcgc ataccgactc
    23341 cggggcgctg gaacgctggc tgcttccagc gtatcagcac gttttcgcca acgtctttaa
    23401 tggcaatctg gcgaaatacg atccctggca tgcggcacat cgtacggaag ttgaagagta
    23461 cacggtggac aacaccacca aatgttccgt gtttcggaca ttccagggct ggactgcgct
    23521 ctcggatatg ctgcctggtc aggggttgct gcacgtcgtg cccattcctg aagccatggc
    23581 gtacgtactg ttacgtccgc tgcttgatga tgtgccggag gatgaactgt gcggcgtagc
    23641 gcccggaaga gtgttgccgg tatcagagca atggcatcca ctgttaattg aggcgttaac
    23701 cagcattcca aaactcgagg ccggagactc cgtctggtgg cactgcgacg tcatccattc
    23761 cgttgccccc gttgaaaatc aacagggttg gggcaacgtg atgtacattc ctgcggcacc
    23821 gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag gccgcactgg aaaaaggcgc
    23881 atcgccgggc gacttcccgc gcgaggacta tgaaacaaac tgggaaggac gctttacgct
    23941 tgccgacctc aacattcacg gtaagcgagc gttgggcatg gatgtttgag agttaatgga
    24001 agtgaattga tgtgggatta agaaaatcga ttcgacgtta ctcagattgc tgacaagtgc
    24061 gctttcttaa tgcctgatgc ggcgttaacg ccttatccgg cctacaaagt cctgcagatt
    24121 caatatatta caggtactgc gtaggcctga taaacgtagc gcatcaggct attttgcatt
    24181 tgtcatcagc cgtcgtcacc ccgacagtat cggggtgacg gataatcact tactcgatcc
    24241 ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt tccggggtga
    24301 tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa acaaagatca
    24361 gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc atatccaggt
    24421 ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac ttaccaaaca
    24481 gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg tcgtcctggc
    24541 tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc
    24601 actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg tggtcctggg
    24661 cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc ggttgcagat
    24721 cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc agtaccgcaa
    24781 gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg ttatcaaacc
    24841 ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc tgttcaaaac
    24901 ggataaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt ttatcaatct
    24961 ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga
    25021 aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc agacgttcac
    25081 gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg cgcagctcgc
    25141 cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg tcgtgcgaga
    25201 tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga atggtgtcga
    25261 tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggctcagca aacagcgcct
    25321 gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg ccgtagtgct
    25381 gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc gcagagtaac
    25441 cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct tcttcactca
    25501 tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac tctttatgcc
    25561 ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc tgacgcagct
    25621 taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca aggtcgccgc
    25681 cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg ccgtaacggt
    25741 tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg aactgcatgg
    25801 tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa accgcgcatt
    25861 atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc aagccaaagg
    25921 caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa cttataatgc
    25981 gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat ctcgcttaac
    26041 atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg cttttgcagt
    26101 accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt tggtcagaat
    26161 caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc cgcggagtac
    26221 cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt cctgccgaaa
    26281 ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt tcatgaaggc
    26341 atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac caacaccgtt
    26401 atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa ctggaccacc
    26461 aaagttgagc ataagaaagc aggcccgacc tggacgccga ccgccaaaat gcacgcagag
    26521 taccgcgctg cgggcgaacc gctaccggct gtcgttccgg caggtccgga taacccgatg
    26581 ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa cgccaacttc
    26641 ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga catcaaattc
    26701 ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc ggtaaaagcg
    26761 accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc taccaccgaa
    26821 gcccagtttg aaggtcagga aatcgtgcca attaccctga cgaagagcgt gcagacagtg
    26881 accggtcagc cagatgttga ccaggttgtt cttgatgaag cgatcaaaaa tcgttccggg
    26941 atgccggttc gcctgaatta atcgtcaaaa cgtaaaacaa aggcggactc ataatccgcc
    27001 ttttttattt gccagacctt accaggccgg gatcataact aacaaaacgt atcccaccag
    27061 cgccgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga tgcggcgatc
    27121 gttcgccata cgtagcgcaa tcaaatttgc cagcgatcct ggtaataaac caaagccccc
    27181 cacgtttacc gcccatgcca gtaataaaga cggaggtaca taattgagca gcaaaatggt
    27241 actcggcacg ttactgatca cctgcgataa accgactgcc gttaaccata acccgggttc
    27301 agataaatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg tcagtaaatg
    27361 gacgtcgata aacatcgcca taaacaccag cagcaacgtc cagtccacac tcaacaccac
    27421 gcgacgcgcc agcagcacaa agcccgccgc cacaatcacc agtccccaca gctcttgttt
    27481 gaactccagc gccgtcagaa agacgatata cagccccaga caattccaca ccagccgcgg
    27541 tttccactcc ggtgtttgca cccccgtatg gtattgcagc gcctttccag ggaaacaaca
    27601 ccagcacaac agcaggagtg tcagcatcat tgcgccagcc atcggtgcca tttgggcaat
    27661 aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg ggttgccaat
    27721 tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca gccgattaac
    27781 cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa acagcgcgac
    27841 atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca tcgccagccg
    27901 acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat aaccgcttaa
    27961 ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt gccagtcgat
    28021 agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga taccaacaag
    28081 aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca ggctcatttg
    28141 gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact aacatggtgc
    28201 tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac caacaagaaa
    28261 ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc ttctgccgtt
    28321 aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag ccgcttaagc
    28381 attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac ctgacgagct
    28441 tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat aagctcgcgc
    28501 cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt cactaactgc
    28561 gtcacttttt ttgctattgg cgtacctgcg cgacgactca ttgtgcttcc tcattatggt
    28621 gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg cgaaggtaca
    28681 aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct gtatgtaatg
    28741 caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga atgagttcaa
    28801 gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc tgctctctat
    28861 gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttcagata aatagcggcg
    28921 tcgcggtacg ccgcttcact cctgctttta tgcaggcata acgcgttttg gtctgaaaaa
    28981 aaccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc agtagttgac
    29041 ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct cgcggatgcg
    29101 gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc agggtaagca
    29161 catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg cttgttaccc
    29221 gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta ttaattaacc
    29281 tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct attttgcttt
    29341 tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt tcgctggtgg
    29401 cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat acattgctgg
    29461 cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt ccgtggtaca
    29521 gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg cgaaaaaaag
    29581 agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc agtgtggcga
    29641 cattttttgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa ctgaaagata
    29701 ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac gaggtgattg
    29761 aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg gtcactgccg
    29821 ttaagccgaa gtatcagacc tatgtggtag tgatcggtga aagcgcgcgt cgcgatgccc
    29881 tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt aacggtttga
    29941 tatttgctga ctacattgct gccagtggct ccacgcagaa atcgcttggc ttaacgctca
    30001 atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg gcaaatcgcg
    30061 cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac gatactgcta
    30121 tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt aattttgaag
    30181 cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg ctggcgcaag
    30241 agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg caggcctgcg
    30301 acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg tgctatctct
    30361 ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta cgcaacagcg
    30421 gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa gagcgcggca
    30481 aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag gtgcctttta
    30541 tggtcatttc cagcggcgat aaagcgcatc gggtgattaa agcccgccgc tcagccaatg
    30601 acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata aatatcaaat
    30661 acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc cagttacaga
    30721 aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa aaacaaaaat
    30781 ccgccccgag aggcggattt ttatatcacc aaagtgatta gaagcggtaa ccaacaccgg
    30841 caatccaagt gcctacgtca acgctacgaa tacggctctg ctcgtaagag aagtccagag
    30901 caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg tagtcgctgg
    30961 tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc acacccacta
    31021 caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg atgccgtagt
    31081 actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg taagtgaaag
    31141 aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg ttgaaaccgc
    31201 ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt acggtagaag
    31261 tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct gaaagacatg
    31321 caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt taaatatatc
    31381 aaaaattaat gggaaactct tcgcgatttg tgatgtctaa cgggccattt catgtaacag
    31441 aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag aactgcgtaa
    31501 gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca gactaatgat
    31561 tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa ttttttcaac aaatgctcaa
    31621 ccagcattgg gtatatccag tacactccac gctttactta agtctagata tttgtgggag
    31681 aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt accaatagtc atattgctcg
    31741 ttgccatggc gtctattcag ggtggagcct cgttagctaa gtcacttttt cctctggtga
    31801 gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac gctgatcctc atcgcgttct
    31861 ttaagccatg gcgactgcgc tttgccaaag agcaacggtt accgctgttg ttttacggcg
    31921 tttcgctggg tgggatgaat tatctttttt atctttctat tcagacagta ccgctgggta
    31981 ttgcggtggc gctggagttc accggaccac tggcggtggc actgttctct tctcgtcgcc
    32041 cggtagattt cgtctgggtt gtgctggcgg ttcttggtct gtggttcctg ctaccgctgg
    32101 ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct ggcactgggg gccggggctt
    32161 gttgggctat ttacatttta agtgggcaac gcgcaggagc ggaacatggc cctgcgacgg
    32221 tagcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc aattggagcg cttcaggctg
    32281 gtgaagcact ctggcactgg tcggttattc cattgggtct ggctgtcgct attctctcga
    32341 ccgctctgcc ttattcgctg gaaatgattg ccctcacccg tttgccaaca cggacatttg
    32401 gtacgctgat gagcatggaa ccggcgctgg c
//
LOCUS       DAJTNV010000041        32142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNV010000041 DAJTNV010000000
VERSION     DAJTNV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO199_001953"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO199_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO199_001954"
     CDS             1127..1234
                     /locus_tag="OO199_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536002.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO199_001955"
     CDS             complement(1287..2045)
                     /locus_tag="OO199_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5536003.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO199_001956"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO199_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5536004.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO199_001957"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO199_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5536005.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO199_001958"
     CDS             3758..4618
                     /locus_tag="OO199_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5536006.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO199_001959"
     CDS             complement(4659..5195)
                     /locus_tag="OO199_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5536007.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTLEQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO199_001960"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO199_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5536008.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO199_001961"
     CDS             6173..6763
                     /locus_tag="OO199_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5536009.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="OO199_001962"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="OO199_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8290..9106)
                     /locus_tag="OO199_001963"
                     /pseudo
     CDS             complement(8290..9106)
                     /locus_tag="OO199_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="OO199_001964"
     CDS             complement(9383..9646)
                     /gene="cedA"
                     /locus_tag="OO199_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5536010.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9829..12090
                     /gene="katE"
                     /locus_tag="OO199_001965"
     CDS             9829..12090
                     /gene="katE"
                     /locus_tag="OO199_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5536011.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="OO199_001966"
     CDS             complement(12137..12895)
                     /gene="chbG"
                     /locus_tag="OO199_001966"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5536012.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12908..14260)
                     /gene="celF"
                     /locus_tag="OO199_001967"
                     /gene_synonym="chbF"
     CDS             complement(12908..14260)
                     /gene="celF"
                     /locus_tag="OO199_001967"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5536013.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNSAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="OO199_001968"
                     /pseudo
     CDS             complement(14365..15207)
                     /gene="chbR"
                     /locus_tag="OO199_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="OO199_001969"
     CDS             complement(15215..15565)
                     /gene="chbA"
                     /locus_tag="OO199_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5536014.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="OO199_001970"
     CDS             complement(15624..16973)
                     /gene="chbC"
                     /locus_tag="OO199_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5536015.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="OO199_001971"
     CDS             complement(17058..17378)
                     /gene="chbB"
                     /locus_tag="OO199_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5536016.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="OO199_001972"
     CDS             complement(17677..18015)
                     /gene="osmE"
                     /locus_tag="OO199_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5536017.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18217..19044
                     /gene="nadE"
                     /locus_tag="OO199_001973"
     CDS             18217..19044
                     /gene="nadE"
                     /locus_tag="OO199_001973"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5536018.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19274..20161
                     /gene="cho"
                     /locus_tag="OO199_001974"
     CDS             19274..20161
                     /gene="cho"
                     /locus_tag="OO199_001974"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5536019.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20121..20696)
                     /gene="ves"
                     /locus_tag="OO199_001975"
     CDS             complement(20121..20696)
                     /gene="ves"
                     /locus_tag="OO199_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5536020.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20899..21384)
                     /gene="spy"
                     /locus_tag="OO199_001976"
     CDS             complement(20899..21384)
                     /gene="spy"
                     /locus_tag="OO199_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5536021.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21714..22682)
                     /gene="astE"
                     /locus_tag="OO199_001977"
     CDS             complement(21714..22682)
                     /gene="astE"
                     /locus_tag="OO199_001977"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5536022.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22675..24008)
                     /gene="astB"
                     /locus_tag="OO199_001978"
                     /pseudo
     CDS             complement(22675..24008)
                     /gene="astB"
                     /locus_tag="OO199_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24005..25483)
                     /gene="astD"
                     /locus_tag="OO199_001979"
     CDS             complement(24005..25483)
                     /gene="astD"
                     /locus_tag="OO199_001979"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5536023.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25480..26514)
                     /gene="astA"
                     /locus_tag="OO199_001980"
     CDS             complement(25480..26514)
                     /gene="astA"
                     /locus_tag="OO199_001980"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5536024.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26511..27731)
                     /gene="astC"
                     /locus_tag="OO199_001981"
                     /pseudo
     CDS             complement(26511..27731)
                     /gene="astC"
                     /locus_tag="OO199_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28177..28983
                     /gene="xthA"
                     /locus_tag="OO199_001982"
     CDS             28177..28983
                     /gene="xthA"
                     /locus_tag="OO199_001982"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5536025.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29150..29858
                     /locus_tag="OO199_001983"
                     /pseudo
     CDS             29150..29858
                     /locus_tag="OO199_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29863..30540
                     /gene="ydjY"
                     /locus_tag="OO199_001984"
     CDS             29863..30540
                     /gene="ydjY"
                     /locus_tag="OO199_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5536026.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30555..31262
                     /locus_tag="OO199_001985"
     CDS             30555..31262
                     /locus_tag="OO199_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5536027.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31262..31810
                     /locus_tag="OO199_001986"
     CDS             31262..31810
                     /locus_tag="OO199_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5536028.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31820..>32142
                     /locus_tag="OO199_001987"
     CDS             31820..>32142
                     /locus_tag="OO199_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5536029.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8248 a         8242 c         7611 g         8041 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttccaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcaag cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaatcatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat
     8401 gtccaactta tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac
     8461 ctcatacccg agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac
     8521 accacttccc attcccggaa gatttagtgg attaaaaaga agattgatat taagcattga
     8581 tattatcgaa ggaaacttta ggagactctc attttttttg aatcgcagca aaaatgctcc
     8641 cggcaggatg tatataattt ttttcatagt atatagtcct taaataatga ataaccaacc
     8701 tattttttat attgatcaaa ttcgtcattt tttcgggtcg gaattcacag gtaaaatcca
     8761 tcccaacaaa attctcatta atatctctta tccgaaactc actgccataa agattattaa
     8821 ttatcactct atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc
     8881 ctagctttga ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg
     8941 actctgctat ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca
     9001 tggtagactc aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac
     9061 cataattact ctgtattaag tttgtagaaa acatctcccg cctcatattg ttaacaaaat
     9121 tattatctta tttaaatcta agtcatttac aatataagtt taagagcgac gccacaggat
     9181 gaactatcaa aaataactca tcatgattag caaaacttaa ccattttaaa ataaataaat
     9241 aattaaagaa aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta
     9301 caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcctgt aactcccggc
     9361 aaaaccggga ggtatgtaat ccttactcag tcacttcccc ttcctggcgg atctgatttg
     9421 cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca
     9481 tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct
     9541 ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt
     9601 tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa
     9661 aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac
     9721 cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt
     9781 tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat
     9841 aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg
     9901 gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg
     9961 ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa
    10021 cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag
    10081 ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg
    10141 ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa
    10201 cgtattgttc atgcacgcgg ctcagcagct cacggttatt tccagccata taaaagctta
    10261 agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt
    10321 aggttctcta ccgttcaggg tggagctggc tctgccgata ccgtgcgtga tatccgtggt
    10381 tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca
    10441 atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa
    10501 ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct
    10561 ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc
    10621 agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg
    10681 aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg
    10741 gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa
    10801 gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa
    10861 gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg
    10921 gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc
    10981 gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac
    11041 gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag tcgtcttggt
    11101 gggccgaatt tccatgagat tccgattaac cgcccgacct gcccttacca taatttccag
    11161 cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattatga accgaactcg
    11221 atcaacgata actggccgcg cgaaacacca ccggggccga aacgcggcgg ttttgaatca
    11281 taccaagagc gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat
    11341 tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc
    11401 gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt
    11461 gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc
    11521 gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag
    11581 gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg
    11641 attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccatgaag
    11701 gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac
    11761 ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg
    11821 gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactat
    11881 ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt
    11941 aaagcaacaa tcaaggtagc tgaccagggt gaagaaggga ttgtggaagc tgacagcacc
    12001 gacggtagtt ttatggatga actgttaacg ctgatggcag aacaccgcgt gtggtcacgc
    12061 attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc cctggggagt
    12121 gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca cgctcagcaa
    12181 ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt gggaaacagt
    12241 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaag
    12301 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
    12361 caccataaaa cgcgctactg aatccctgcg aactgcgcag attaaccggt aaatcaccag
    12421 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
    12481 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
    12541 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
    12601 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
    12661 cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc
    12721 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
    12781 tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
    12841 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
    12901 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
    12961 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
    13021 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
    13081 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
    13141 gcgtaatgcg cggatgtggc gtcgcgccat cgtgccccag cttacaggtc atttctaccg
    13201 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
    13261 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
    13321 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta tacagctcaa
    13381 aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg tagtattcgc
    13441 ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa gaacacggca
    13501 acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata tttttaacgc
    13561 cagatacttt taactgcccg gatgccacac catcaagcaa ttcggcaaag cgcgacttgc
    13621 catttaccag cacatcctta atgaacacca tatggttgag gccaaacaaa tcgatagata
    13681 aatcatcgct gtctttcagc atcagaacat cgtgaataaa catcttcatg ccgatcggaa
    13741 tattacacac gccgataaaa cgtttaaatc cggtatgacg ataaacggct tcagtaacca
    13801 ttcccgccga gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
    13861 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacaaaccg cccgcgccgt
    13921 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcacgcgccg
    13981 gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
    14041 aatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
    14101 gatcgaaaat aatatccagt ttagctttgc caccctcgac atccaccagc cataattcgc
    14161 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
    14221 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
    14281 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
    14341 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatatct
    14401 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
    14461 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gtttttttgc
    14521 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
    14581 cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
    14641 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actacggtac
    14701 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
    14761 catctcaaca aactcttcca gccctgtttc gcggaaattt aatgaagaaa tcactgtttc
    14821 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcaacaa agcaataagg
    14881 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatgcg
    14941 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
    15001 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
    15061 tagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata
    15121 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
    15181 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
    15241 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
    15301 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
    15361 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gctttcgctg
    15421 cggcaaaatc gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc
    15481 cagagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
    15541 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
    15601 ccgtaccggc atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta
    15661 tcaatcgcat tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
    15721 atgccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
    15781 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
    15841 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
    15901 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
    15961 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
    16021 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
    16081 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
    16141 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
    16201 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc
    16261 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
    16321 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
    16381 gaaagaataa taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg
    16441 ctgtcgggca gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
    16501 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
    16561 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
    16621 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
    16681 gccattaaag acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga
    16741 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaaacgac
    16801 ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc cgcaagggtt
    16861 aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg ctttcctatt
    16921 ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact catagaaaaa
    16981 tacccttaaa aaccgcaatt taaatattac ggtattgatt tatgaaataa ctctttgacg
    17041 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
    17101 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
    17161 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
    17221 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
    17281 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga gatagacatg
    17341 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
    17401 atctgtcagc cagacactcc gcaagccttg acctgcttcc atgctctggg taacttgcga
    17461 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
    17521 tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc ttcacatttc
    17581 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
    17641 ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt
    17701 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
    17761 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
    17821 gcgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
    17881 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg
    17941 atcataagct gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
    18001 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
    18061 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
    18121 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
    18181 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
    18241 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
    18301 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
    18361 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
    18421 caggaaactc gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
    18481 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
    18541 gttaatatca agggcgcggt attggctagc gagcaggcat tgcgggaagc aggcattgaa
    18601 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
    18661 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
    18721 ggattcttca ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac
    18781 aaacgccagg gtaaacagtt actgacggca ttaggttgcc cggaacacct ttataagaaa
    18841 gccccaactg ccgatcttga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
    18901 gtgacctatg acaatatcga cgactatctg gaagggaaaa acctacctga acaggtcgcc
    18961 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaacgtt
    19021 ttcgatgatt tctggaaaaa gtaataattt gcacctattg gattgtgcga aaaagagtaa
    19081 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
    19141 atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt gtaacctttg
    19201 tcatcaaccc gcctcttttt taatcgcctc ccgcctgtta cactggatag ataaccagca
    19261 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
    19321 aatttatgaa tatcccgaac atttacattc attccttaat gacttaccca cccgaccagg
    19381 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
    19441 tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca tgctacggca
    19501 atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc
    19561 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
    19621 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
    19681 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
    19741 gcagagcatc gctgatgaac aaaaactttg ttatggcctg ttgggactgg aaccgttaag
    19801 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
    19861 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgagtggt
    19921 gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa tgactcaata
    19981 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
    20041 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
    20101 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
    20161 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
    20221 aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta
    20281 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
    20341 actttcgcct tacagacatc caggcgcgtc ataatattga aatccatcga catctgccct
    20401 gccgtcagtt tcgcttttac cacctggtcc gccgtaaagg caaaaggctg taacggtttt
    20461 aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
    20521 actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
    20581 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
    20641 ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
    20701 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatgat tatcggcaac
    20761 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag
    20821 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
    20881 agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
    20941 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat
    21001 gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
    21061 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
    21121 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
    21181 gatttcgtgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
    21241 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc
    21301 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
    21361 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
    21421 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
    21481 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
    21541 aagaaaattt gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta
    21601 tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
    21661 taatttataa gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
    21721 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac
    21781 acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt
    21841 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
    21901 gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg
    21961 acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga
    22021 tcgttttgcc caaagggcaa cgctttgcca agttccaacg tacagaccag cgcgccaaaa
    22081 tgtctgacgc tgaaatgggt aaacgtacca ccaggctcct gatggaacac cagcgcctcc
    22141 agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
    22201 aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccaa
    22261 cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc
    22321 cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcggttcata
    22381 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc
    22441 aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt
    22501 tccacaggtg ccgtctcatt accgtggatc cccgcagaaa tcaccagcgc gccttgcggt
    22561 ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg
    22621 atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc
    22681 attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
    22741 tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt
    22801 aagacgatcg cggtagtaac gatccaccca gtcattgagc acattaaaca aggtatcgtt
    22861 catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaataccc cccgcaaccg
    22921 caggcacgcc gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc
    22981 actaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgctc
    23041 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct
    23101 gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat
    23161 cgccataaaa ccgttcaccc gcgtgccagt aactgtgact ggcgagcgaa cgcctgttgg
    23221 tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgctctgg
    23281 tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattaagc
    23341 cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta
    23401 tcattgcctt cttctcgccc gtagacaaaa agtagcatac ccggttcacc gtaatgaccg
    23461 ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc
    23521 gaatggacgc taaatttctc ttcgtcctta aaaatcgctt ttaacagcga ttcagtgacg
    23581 ggcgcttcca gcgaacggtg aaatttattg ttcaggttgg caacggtgag atgcactttg
    23641 ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa
    23701 gcggaactga cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc
    23761 tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc
    23821 gggatcacgg cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt
    23881 tttgccgcca gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca
    23941 aacgacaggc ccgcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag
    24001 gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtg gcgggcaatg
    24061 ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg
    24121 cgctggggcg atggttaccg gaagcaccaa tgccgccgaa tggggcggta ctggcagcac
    24181 cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga
    24241 atttttcccg ctcgggggaa accagaccgc aaaagagtcc gaagcgagtg ttgttcgcca
    24301 tccgaatcgc ctcatcaaaa gtatcataac gccagacgcg caaaagcggc ccgaacacct
    24361 cttcatccgg cacaccgcca acgcccgtca tttcaatgat ccctggtgtc agcaacgatg
    24421 tccctgcttg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct
    24481 gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct
    24541 gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc agactagcaa
    24601 gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt
    24661 gcccgactgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca
    24721 cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct
    24781 gaccggagag ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac
    24841 cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt
    24901 tcagcacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc
    24961 acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga
    25021 tatgtccgtt cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt
    25081 gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac
    25141 gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg
    25201 ccgcttccca gcgcggctta cccgtttctc tggcaataat cgcggttaat tcggctttat
    25261 tgctttccag caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc
    25321 gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc tgagcggcat
    25381 cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg
    25441 cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc
    25501 gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc
    25561 ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg
    25621 gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc
    25681 tgccacttcc accagccgac ttttacggat ggcgcgcacg cggtcgatgt cacactcaag
    25741 cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag
    25801 caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg
    25861 ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg
    25921 cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag
    25981 gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac
    26041 tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa
    26101 atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct
    26161 gccggtgtga tcgttactaa gaaacagcgt cggcaatgcg ttatagacat tcagctcttt
    26221 tgaggcgtga accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc
    26281 cacctcaata gcgcaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac
    26341 atagccctgc tcgcttttgg gcagttcacc ttgccaggtt ttgattgccc tttcgatacg
    26401 cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc
    26461 aagctgcatc agcgccgaga catctgagcg ctcaacggga cggatgacca tcatgatgaa
    26521 cctcggctaa caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct
    26581 tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc
    26641 atcacgcctg ctttcgccgc ttccagagag atctgtttcg cttgcccggc gtaatcggca
    26701 ttcagtacac agccaatcaa caaacctaag ccgcgaactt cactgaacaa accatagcgg
    26761 tgattaatag tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc
    26821 atctctggtg tgttgatgag ctccagcact ttgcctgcta ccgccgaggc cagcggatta
    26881 ccgccatagg tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc
    26941 aacaacgcac cgaccgggaa accgccgccc agcgctttgg cggtggttaa cagatcgggc
    27001 gtcacgccgt agtgcatata ggcatacagc tcaccagtgc gcccgacgcc ggtttgtacc
    27061 tcatcaaaaa tcagcaaggc attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac
    27121 gcgttgctgg ctggcaccac accgccttcg ccctagatgg gctcgacaat caccgcacag
    27181 gtggcgtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata
    27241 tccgccggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta
    27301 aacagcgtgc gaccatgaaa cgcatttttg aacgccacga tgccactctt atggctgccg
    27361 tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg
    27421 gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc
    27481 agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc
    27541 agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg
    27601 aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt
    27661 ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt
    27721 ggctgagaca tagcgacctc tacagtaaat aattcgttat ttgcatgtta acaataagta
    27781 atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa
    27841 acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca
    27901 tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg
    27961 cagaagtaaa atataattct agaattgtga tcattgacga aatttactgg aaattactgc
    28021 gccattctga cgcagcgcgc accaaaagcg ggcatttttt gcgccatcat tgacatcatt
    28081 aacaaccatc gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca
    28141 tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc
    28201 aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat
    28261 gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg
    28321 aagctcggct acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg
    28381 accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag
    28441 cggcggatta ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt
    28501 tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt
    28561 tatcagaatc tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt
    28621 atgggcgata tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt
    28681 aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac
    28741 aggctgatga gctggggggt ggtcgatacc ttccgccatg ctaatccgca aacagcagat
    28801 cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc
    28861 gacctgctgc tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat
    28921 gaaattcgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc
    28981 taatttagca gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa
    29041 caaactgtca gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta
    29101 gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga
    29161 gcgtaacttt ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt
    29221 cggtttattc gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg
    29281 attgttcggt tatagcctct atattctgtt attcatcaat gccccctctt tctgttacca
    29341 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg acacaactct
    29401 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    29461 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    29521 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    29581 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    29641 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    29701 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    29761 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    29821 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    29881 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    29941 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    30001 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    30061 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    30121 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    30181 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    30241 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    30301 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    30361 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    30421 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    30481 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    30541 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    30601 tcatcatcct gttattcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    30661 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    30721 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    30781 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    30841 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    30901 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    30961 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    31021 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    31081 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    31141 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    31201 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    31261 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    31321 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    31381 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    31441 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    31501 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    31561 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    31621 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    31681 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    31741 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    31801 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    31861 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    31921 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    31981 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    32041 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    32101 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aa
//
LOCUS       DAJTNV010000042        31766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNV010000042 DAJTNV010000000
VERSION     DAJTNV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO199_001988"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO199_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5536030.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO199_001989"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO199_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5536031.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO199_001990"
     CDS             2630..3391
                     /locus_tag="OO199_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5536032.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO199_001991"
     CDS             complement(3442..4326)
                     /locus_tag="OO199_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5536033.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO199_001992"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO199_001992"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5536034.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO199_001993"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO199_001993"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5536035.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO199_001994"
     CDS             6202..6474
                     /locus_tag="OO199_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5536036.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO199_001995"
     CDS             6844..7920
                     /locus_tag="OO199_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5536037.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO199_001996"
     CDS             7947..9326
                     /locus_tag="OO199_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536038.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO199_001997"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO199_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO199_001998"
     CDS             10409..11245
                     /locus_tag="OO199_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5536039.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO199_001999"
     CDS             11250..12197
                     /locus_tag="OO199_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5536040.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO199_002000"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO199_002000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5536041.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO199_002001"
     CDS             13324..14082
                     /locus_tag="OO199_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5536042.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO199_002002"
     CDS             14200..15558
                     /locus_tag="OO199_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536043.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO199_002003"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO199_002003"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5536044.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO199_002004"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO199_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5536045.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO199_002005"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO199_002005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5536046.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO199_002006"
     CDS             19503..20054
                     /locus_tag="OO199_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5536047.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO199_002007"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO199_002007"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5536048.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="OO199_002008"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="OO199_002008"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5536049.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="OO199_002009"
     CDS             23307..24347
                     /locus_tag="OO199_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5536050.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="OO199_002010"
     CDS             complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="OO199_002010"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5536051.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26043..26315
                     /locus_tag="OO199_002011"
     CDS             26043..26315
                     /locus_tag="OO199_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5536052.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="OO199_002012"
     CDS             complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="OO199_002012"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5536053.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26775..27395
                     /locus_tag="OO199_002013"
     CDS             26775..27395
                     /locus_tag="OO199_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5536054.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="OO199_002014"
                     /pseudo
     CDS             complement(27404..28711)
                     /gene="ynjE"
                     /locus_tag="OO199_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28778..29431)
                     /locus_tag="OO199_002015"
     CDS             complement(28778..29431)
                     /locus_tag="OO199_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5536055.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29431..30948)
                     /locus_tag="OO199_002016"
                     /pseudo
     CDS             complement(29431..30948)
                     /locus_tag="OO199_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30921..31766)
                     /locus_tag="OO199_002017"
                     /pseudo
     CDS             complement(30921..31766)
                     /locus_tag="OO199_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7959 a         7604 c         8240 g         7963 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcaaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgccgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc taggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatctg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atatggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gcttcgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatggggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcagca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcacgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatctg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
    25681 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
    25741 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
    25801 gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg tttatgtcat
    25861 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
    25921 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
    25981 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
    26041 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
    26101 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
    26161 agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg
    26221 cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa acccttagca
    26281 ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
    26341 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
    26401 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
    26461 gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt cgatgtccaa
    26521 ttcttcgttt aactcacgca ccagcacctg ctgctggctt tcatccagct cgactttacc
    26581 tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
    26641 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
    26701 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
    26761 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
    26821 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
    26881 ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg gcggcgctag
    26941 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
    27001 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt tacgcgctgg
    27061 tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt
    27121 tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac
    27181 atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg ggattaaccg
    27241 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
    27301 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
    27361 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
    27421 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
    27481 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
    27541 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
    27601 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
    27661 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
    27721 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
    27781 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
    27841 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
    27901 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
    27961 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
    28021 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
    28081 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
    28141 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
    28201 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
    28261 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
    28321 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
    28381 cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
    28441 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctagcagaga
    28501 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
    28561 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
    28621 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
    28681 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
    28741 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
    28801 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
    28861 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
    28921 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
    28981 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
    29041 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
    29101 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
    29161 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
    29221 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
    29281 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
    29341 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
    29401 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
    29461 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
    29521 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
    29581 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
    29641 tgaaaatccc accgcgaagg cagtcagcac agggcgcaac atgagtgggc atttcacgta
    29701 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
    29761 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
    29821 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
    29881 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
    29941 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
    30001 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
    30061 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
    30121 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
    30181 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
    30241 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
    30301 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
    30361 tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc
    30421 attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt
    30481 cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact gcaagggtga
    30541 ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat agtcatatag
    30601 cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat ggggaatggc
    30661 gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc atagcgccac
    30721 aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg acaccagcgt
    30781 tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc gtgtcaacga
    30841 caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa tcaccgccac
    30901 catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc ttgttccagt
    30961 gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg cggcattctt
    31021 gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac agaaggatcg
    31081 ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag gaaattggca
    31141 actaccttcg ccgcagcact ggcattcgcg ttggctggag tggtgacgaa atgcacgttg
    31201 ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc gccgctggcg
    31261 attttttgct gcgcatgtgc ggggttaaag gtcagcgaca ggcgcaatgt gccggctttc
    31321 agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg ccacaaatac
    31381 ggatgcagtg catcaagata ttgctacaag ggcgcagtga cacgggcgaa agtcgcatcg
    31441 tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa ctgttcaaga
    31501 aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg attggcttta
    31561 gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc gcggcgggcg
    31621 ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg cactgaaaaa
    31681 tctccccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt ctccgcccag
    31741 cccgtttgta ataaattggc ctcttt
//
LOCUS       DAJTNV010000043        30861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNV010000043 DAJTNV010000000
VERSION     DAJTNV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            252..1910
                     /locus_tag="OO199_002018"
     CDS             252..1910
                     /locus_tag="OO199_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5536056.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1907..2863)
                     /locus_tag="OO199_002019"
     CDS             complement(1907..2863)
                     /locus_tag="OO199_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5536057.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3014..4129
                     /gene="macA"
                     /locus_tag="OO199_002020"
     CDS             3014..4129
                     /gene="macA"
                     /locus_tag="OO199_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5536058.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4126..6072
                     /gene="macB"
                     /locus_tag="OO199_002021"
     CDS             4126..6072
                     /gene="macB"
                     /locus_tag="OO199_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5536059.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6145..6369)
                     /gene="cspD"
                     /locus_tag="OO199_002022"
     CDS             complement(6145..6369)
                     /gene="cspD"
                     /locus_tag="OO199_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5536060.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6692..7012
                     /gene="clpS"
                     /locus_tag="OO199_002023"
     CDS             6692..7012
                     /gene="clpS"
                     /locus_tag="OO199_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5536061.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7043..9319
                     /gene="clpA"
                     /locus_tag="OO199_002024"
     CDS             7043..9319
                     /gene="clpA"
                     /locus_tag="OO199_002024"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5536062.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9722..9809)
                     /locus_tag="OO199_002025"
     tRNA            complement(9722..9809)
                     /locus_tag="OO199_002025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9773..9775),aa:Ser,seq:gga)
     gene            complement(10063..10281)
                     /gene="infA"
                     /locus_tag="OO199_002026"
     CDS             complement(10063..10281)
                     /gene="infA"
                     /locus_tag="OO199_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5536063.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10566..11270)
                     /gene="aat"
                     /locus_tag="OO199_002027"
     CDS             complement(10566..11270)
                     /gene="aat"
                     /locus_tag="OO199_002027"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5536064.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11312..13033)
                     /gene="cydC"
                     /locus_tag="OO199_002028"
     CDS             complement(11312..13033)
                     /gene="cydC"
                     /locus_tag="OO199_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5536065.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13034..14800)
                     /gene="cydD"
                     /locus_tag="OO199_002029"
     CDS             complement(13034..14800)
                     /gene="cydD"
                     /locus_tag="OO199_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5536066.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14923..15888)
                     /gene="trxB"
                     /locus_tag="OO199_002030"
     CDS             complement(14923..15888)
                     /gene="trxB"
                     /locus_tag="OO199_002030"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5536067.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16432..16926
                     /gene="lrp"
                     /locus_tag="OO199_002031"
     CDS             16432..16926
                     /gene="lrp"
                     /locus_tag="OO199_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5536068.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17061..21089
                     /gene="ftsK"
                     /locus_tag="OO199_002032"
     CDS             17061..21089
                     /gene="ftsK"
                     /locus_tag="OO199_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5536069.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21244..21855
                     /gene="lolA"
                     /locus_tag="OO199_002033"
     CDS             21244..21855
                     /gene="lolA"
                     /locus_tag="OO199_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5536070.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21866..23209
                     /gene="rarA"
                     /locus_tag="OO199_002034"
                     /gene_synonym="mgsA"
     CDS             21866..23209
                     /gene="rarA"
                     /locus_tag="OO199_002034"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5536071.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23300..24592
                     /gene="serS"
                     /locus_tag="OO199_002035"
     CDS             23300..24592
                     /gene="serS"
                     /locus_tag="OO199_002035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5536072.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24832..27276
                     /gene="dmsA"
                     /locus_tag="OO199_002036"
                     /pseudo
     CDS             24832..27276
                     /gene="dmsA"
                     /locus_tag="OO199_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27287..27904
                     /gene="dmsB"
                     /locus_tag="OO199_002037"
     CDS             27287..27904
                     /gene="dmsB"
                     /locus_tag="OO199_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5536073.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27906..28697
                     /locus_tag="OO199_002038"
     CDS             27906..28697
                     /locus_tag="OO199_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5536074.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28733..29359)
                     /locus_tag="OO199_002039"
     CDS             complement(28733..29359)
                     /locus_tag="OO199_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5536075.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29674..30822
                     /locus_tag="OO199_002040"
     CDS             29674..30822
                     /locus_tag="OO199_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536076.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7601 a         7584 c         8313 g         7363 t
ORIGIN      
        1 tcatttgtct gattgacagt gtagtgcaag caaaagattt aatcctttag gcgtaataaa
       61 aaataattta tcatgctaat tatttgattt tgttgttttt gcgggcttat cagcaagagg
      121 gagtataacg cgattattcg ctcatttttc agacaattgc catgcttaaa tgtgatgtca
      181 tcacgtatta gcaaggcctt tcccgttata ctgcgagcgt aaaggataag tcacatattt
      241 ctggagggga tatgattctt gagcgcgttg aaattgtggg ttttcgcggt atcaaccgtt
      301 tgtcgttgat gctcgaacaa aacaacgtcc tgattgggga gaacgcgtgg ggtaaatcca
      361 gcttgctgga cgccttaacc ctgctgctat cgccagaatc agatctctac cattttgagc
      421 gcgacgattt ctggttcccg ccgggagata tcaacgggcg agaacatcat ctgcatatta
      481 ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt tcgccgttat cggccgctgg
      541 aagcgtgctg gacgccatgc accgatggct atcaccgtat tttttatcgt ctggaagggg
      601 agagtgcgga agacggcagc gtgatgacac tgcgcagttt tctcgataaa gacggacatc
      661 cgattgatgt tgaggatatt aacgatcagg cacgccatct ggtgcgttta atgccggtgc
      721 tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa cggcacggtg ccaaatgtcc
      781 ccaatgtgga agtcaccgcg cgccagctcg atttcctcgc ccgtgagttg tcctcacatc
      841 cgcaaaatct ctctgatgga cagattcgtc agggactttc cgcaatggtg cagttgcttg
      901 agcattattt ctctgagcag ggggctggac aggcgcgata tcgtttaatg cggcggcggg
      961 ccagcaatga gcaacgaagc tggcgctatc tggacatcat caaccggatg attgaccgac
     1021 ctggagggcg ctcgtatcgg gttattttgc tcggcctgtt tgctacttta ttgcaggcaa
     1081 aaggcacatt gcgactggat aaagacgcgc gtccgttatt gctaattgaa gatccagaaa
     1141 cccgcctgca tccgattatg ctttccgttg cctggcatct gttgaatctt ttgcctttac
     1201 agcgtattgc taccaccaac tctggcgagc tgctttcgtt aactccagtg gaacatgttt
     1261 gccgcctggt gcgtgaatct tctcgtgttg ccgcctggcg tctggggcca agtggcttga
     1321 gcaccgaaga cagccgccgc atctcttttc acattcgttt taatcgtccg tcatcgctgt
     1381 ttgctcgctg ctggttgctg gtggaagggg aaacggaaac ctgggttatc aatgaactgg
     1441 cgcgtcagtg cggacatcat tttgatgccg aagggatcaa ggtcattgag tttgcccagt
     1501 ccgggctaaa gccactggtt aaatttgccc gccgaatggg gattgaatgg catgtactgg
     1561 tcgatggcga tgaagcaggg aagaaatatg ccgctacggt acgcagcctg ttgaataacg
     1621 atcgggaagc cgaacgagaa catttaacgg cgttaccggc gctggatatg gaacatttta
     1681 tgtatcgcca gggattttcc gatgtgttcc accgcgtggc gcaaatcccg gaaaatgtac
     1741 cgatgaatct acgcaaaatt atctcgaaag cgatccatcg ctcttccaaa cccgatcttg
     1801 ccattgaagt ggcaatggag gcaggacgtc gtggtgtgga ctccgtacca acgctgctga
     1861 aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg cgcggattaa ccgcgaaaca
     1921 tcgtggccat ttgtggctga atagcgtcga gcatctcata gcgccgacgg tattcagccc
     1981 gttttttact ggcgatttcg gcaatctctt ttcgtgctat ctgtgctgga aggcggtaat
     2041 ggcgttcagc atcacatacg ccgccaaccg actcccagaa cgcgttgtaa tcagcatgga
     2101 tcttgccttc tttatcgcga taacgcaggc tgcggtaaat atgcgtttca ttgctgacgg
     2161 caataatctg ctctacctgc aaacgttggg caaacagaca ggccgcttcc atcacgaggc
     2221 gtttgggaaa tagcccgtgg caggctttcg tcgcattctg gatttcctga tgtggaattt
     2281 cccattttgc gccttgcagt ccgccaataa acatcgttct tttcccctga tattcacaca
     2341 gggtaaacgt gatctctgcc agaggaatac cttcgctgtt gcggaacagg attgtgctgt
     2401 caccttcttt atccattgag atcatcatgg tcagctcaag cgtgaactgc tcaccgtttt
     2461 tgccttccag tttagccagt tgcagcccgg gggtattcaa atataagctg aattcttccg
     2521 ccgacataca cccgcggagt aacgcataat ggtaacgtaa cgcctccagc aattgcttac
     2581 ggctaagatt cgcggcaagg taagggcgat gcagacgcac aggcagtcgc ggctggcgcg
     2641 ttaacaacac attgagatta ggccagtggg aaagttcgtt catccactca acgcttaaac
     2701 gcggcataat caacgagcgc agcaaaaatt tctggcgaaa actacagcga tgccagaatt
     2761 tacccggccg acactgtcca cgtgccagac taagaaaaag tgacaggctg ctgagagatt
     2821 cagatggcgt aaaggtccgt tcagttagct gcgacatatt catgaaatca atggttatac
     2881 atgacgtcga tttcaccatt gcgtatctta accaaacatc aatagtgtga ttactaacgt
     2941 aaattttagg gttttgttga tatttcgttg aagttaatga cccggattgg catatggagt
     3001 attcagaata tttatgaaaa agcggaaaac cgtgaagaag cgttacgtta ttgcgctggt
     3061 gatagtcatc gccggactga ttacgttatg gagaattctt aacgcacccg tgccgactta
     3121 tcagacactg attgtgcgcc ccggtgattt acagcaaagc gtgctggcga ccggaaagct
     3181 ggacgcgctg cgtaaggttg acgtgggcgc gcaggtcagc ggtcagttga aaacgctgtc
     3241 ggtggcgatt ggcgataaag taaaaaaaga ccagctttta ggggtcattg atcctgaaca
     3301 ggctgaaaac cagatcaagg aggtcgaagc aacgctgatg gagctacgcg cgcagcggca
     3361 gcaggcggaa gcggagctga aactggcgcg ggtgacgtat tcccgtcagc aacgtctggc
     3421 acaaacgcaa gcggtttcac tgcaggatct cgataccgcc gcgacggaga tggctgtgaa
     3481 acaggcgcaa attggcacca ttgacgcgca aatcaagcgc aatcaggcct ctctcgatac
     3541 ggctaaaacc aatctcgatt acacccgtat cgttgccccg atggctgggg aagtgacgca
     3601 aatcaccact ctgcaaggcc agacggtgat tgccgcacag caagcaccga acattctgac
     3661 gctggcagat atgagcacta tgctggtaaa agcgcaggtt tctgaagcgg atgtaatcca
     3721 cctgaagccg gggcaaaaag cctggtttac ggtgcttggc gatccactga cgcgctacga
     3781 ggggcaaatc aaggatgtgc taccgacgcc ggaaaaggta aacgacgcta ttttctatta
     3841 cgcccgtttt gaagtaccca accccaatgg tttgctgcgg ctggatatga ccgcgcaagt
     3901 gcatattcag ctcaccgatg tgaaaaatgt gttgacgatc cctctgtcgg cgttaggcga
     3961 tccggttggc gataatcgtt ataaagtcaa attgttgcgt aatggtgaaa cacgcgagcg
     4021 tgaagtgacg attggcgcac gtaacgatac cgatgttgag attgtcaaag ggctggaagc
     4081 gggcgatgaa gtggtgattg gtgaggccaa accaggagct gcacaatgac gcctttgctc
     4141 gaattaaagg atattcgtcg cagctatcct gccggtgatg agcaggttga ggtgctgaag
     4201 ggcatcagcc tcaatattta tgcgggtgag atggtcgcga ttgttggcgc ttcgggttcc
     4261 ggtaaatcga ccctgatgaa tattctcggc tgcctggata aggccaccag cggcacctat
     4321 cgcgtagccg gtcaggatat tgccacgctg gacgccgatg cgctggcgca actgcgccgc
     4381 gagcatttcg gctttatttt ccagcgttac catttgcttc cgcatttaac cgccgagcag
     4441 aacgttgaag tacccgccgt ctatgccggt cttgagcgga aacagcgact gcttcgtgcc
     4501 caggagttgc tgcaacggct ggggctggaa gaccgtacag agtattatcc ggcacagctt
     4561 tcgggtggtc agcaacagcg cgtcagcatc gcgcgggcat tgatgaacgg cggtcaggta
     4621 attcttgccg atgaaccgac cggcgcactg gacagccatt ctggcgaaga ggtgatggcg
     4681 atcctgcatc agctgcgcga tcgcgggcat acggtgatta tcgtcaccca cgatccgcag
     4741 gtcgctgctc aggccgagcg ggtgatcgaa attcgcgacg gcgaaattgt gcgcaatcct
     4801 cccgccattg aaaaagtgaa tgttgctggc gggacggagc ctgttgtcaa tacagtgtct
     4861 ggctggcggc agtttgtcag cggttttaac gaggcgctga cgatggcatg gcgggcgctg
     4921 gcagcgaata aaatgcgtac tttactgacc atgctgggga ttattatcgg tattgcgtcg
     4981 gtggtttcca ttgtcgtggt gggtgacgcc gccaaacaaa tggtgctggc ggatattcgt
     5041 tctattggta cgaatactat tgatgtctat cccgggaaag attttggcga tgacgatccg
     5101 caatatcagc aggcgctgaa gtacgacgac ttaatcgcca tccaaaaaca accgtgggtc
     5161 gcctcagcca cacctgccgt ctcgcaaaac ctgcgcctgc gttataacaa tgttgatgtt
     5221 gctgccagtg ccaatggcgt gagcggcgat tattttaatg tctatggcat gaccttcagt
     5281 gaaggaaaca cctttaatca ggagcagctg aacggtcgtg cgcaggtcgt ggttctcgac
     5341 agtaatactc gccgccagct tttcccccat aaagcagatg tggttggcga ggtgattctg
     5401 gtcggcaata tgcccgccag agtcattggt gtggcggaag aaaaacagtc gatgtttggt
     5461 agcagtaaag tgctgcgtgt ctggctacct tacagcacga tgtccgggcg agttatgggc
     5521 cagtcgtggc ttaactccat tactgtcagg gtgaaagaag gatttgacag cgccgaggcg
     5581 gaacagcaac tcacgcgttt actttcactg cgccacggta agaaggattt ctttacctgg
     5641 aacatggacg gcgtcttgaa aactgttgaa aagaccacac gtactttaca actgtttctg
     5701 acgctggtgg cggtgatttc gctggtggtg ggcggtattg gtgtaatgaa tattatgctg
     5761 gtgtcagtga ccgagcggac gcgggaaatt ggcattcgca tggctgtagg tgcgcgagca
     5821 agcgatgttt tgcaacagtt cctgatcgaa gccgtactgg tttgcctggt cggtggcgcg
     5881 ttgggaataa cactgtcact gttaattgct ttcaccttgc agcttttctt acccggctgg
     5941 gagattggtt tttcaccgtt ggcgctgctg ctggcgtttc tctgctcgac ggccaccggg
     6001 attttatttg gctggttacc cgcacgaaat gcggcacgac tggatccagt agatgctctg
     6061 gcacgggagt aatttttgag ataaaaatgc cagccgatcg ggctggcatt ttgcctttag
     6121 gatgtacaca atgagacaga agagctatgc gactgccgct tctacttcga cgggcacaat
     6181 aacactggcg tgattgcctt ttggcccctg gtggacatca aactgaacgg attgtccagc
     6241 ttttagcgtt ctgtaaccat ccatctgaat ggtggaataa tgagcgaaaa tatcttcgcc
     6301 gccgccttca gggcagatga aaccaaaccc tttggcattg ttgaaccact taacagtacc
     6361 cttttccatg cttcgacatc cttcgcaaat cttatacaag taagatggaa taaaccgggg
     6421 tcagagaggg ggctgttcaa aacctcgcca actctagaaa tacaatttag agaattaggg
     6481 cgagccgtca agcatttgac aggggccagg gggcaggtat gaatcaaaaa tttgaagcag
     6541 ttaacgctat tgacaggaat gtgacagatg tcgctgatgc caacgataga tgatagttat
     6601 ctatcatgtg gagtagattg gtcaggcaaa taagctcttg tcagcggcag ggcgttctgc
     6661 cgataaccgt aaccgaagat gataactgac aatgggtaaa acgaacgact ggctggactt
     6721 tgatcaactg gcggaagaaa aagttcgcga cgcgctaaaa ccgccatcta tgtataaagt
     6781 gatattagtc aatgatgatt acactccgat ggagtttgtt attgacgtgt tacaaaaatt
     6841 cttttcttat gatgtagaac gtgcaacgca attgatgctc gctgttcact accaggggaa
     6901 ggccatttgc ggagtcttta ccgccgaggt tgcagaaacc aaagtggcga tggtgaacaa
     6961 gtacgcgagg gagaatgagc atccattgct gtgtacgcta gaaaaagcct gaatgcaggc
     7021 ataaaaattg ggggaggtgc ctatgctcaa tcaagaactg gaactcagtt taaatatggc
     7081 tttcgccaga gcgcgcgagc accgtcatga gtttatgacc gtcgagcact tgttactggc
     7141 gctgctcagt aacccatctg cccgggaggc gctggaagcg tgttctgtgg atttggttgc
     7201 gctccgtcag gaactggaag cctttattga acaaaccaca cccgttctgc ctgccagtga
     7261 agaggagcgc gacacacagc cgacgctgag ttttcagcgt gtactgcaac gtgcggtctt
     7321 ccatgtccag tcctccggtc gcaatgaggt taccggtgca aacgttctgg tcgctatctt
     7381 cagcgaacag gagtcgcagg cggcatatct gttgcgtaaa catgaagtca gccgtctcga
     7441 tgtggtgaac tttatctctc atggcacgcg taaagacgag ccgacacagt cttctgatcc
     7501 tggcagccag ccaaacagcg aagaacaagc tggtggggag gaacgtatgg agaatttcac
     7561 gacgaacctg aaccagcttg cgcgtgtggg cggaatcgac ccactaattg gtcgtgagaa
     7621 agagctggag cgtgctattc aggttctctg ccgtcgccgt aaaaacaacc cgctgctggt
     7681 gggggaatct ggtgtcggta aaaccgcgat tgcagaaggt cttgcctggc gaattgttca
     7741 gggtgatgtg ccggaagtga tggctgactg tacgatttac tctctcgata tcggttctct
     7801 gttagcgggc acaaaatatc gcggcgactt tgaaaaacgt tttaaagcgt tgctcaagca
     7861 gctggagcag gacactaaca gcatcctgtt tattgatgag atccacacca ttatcggtgc
     7921 gggtgcagcg tctggtggtc aggtcgatgc ggctaaccta atcaaaccgt tgctctccag
     7981 cggtaaaatt cgcgtaattg gttcgacaac ctatcaggag ttcagcaaca ttttcgagaa
     8041 agaccgtgct ctggcgcgtc gcttccagaa aattgatatt actgaaccgt cgatcgaaga
     8101 aactgttcaa atcatcaatg gcctgaaacc gaagtatgaa gcgcaccacg acgtgcgtta
     8161 taccgcaaaa gcggtgcgtg cggcggtaga gctggcggtg aaatacatta acgatcgtca
     8221 tctgccggat aaagccattg atgttatcga cgaagcgggc gctcgcgcac gtctgatgcc
     8281 ggtaagcaaa cgcaagaaaa ccgttaatgt ggcggatatt gagtccgtgg tggcccgtat
     8341 tgcacgcatt ccagagaaga gtgtttctca gagtgaccgc gataccctga aaaaactcag
     8401 cgatcgcctg aaaatgctgg tcttcggcca ggataaagcc attgaggcgc tgactgaagc
     8461 cattaagatg gcgcgtgcag gtttaggtca cgaacataaa ccggtcggtt cgttcctgtt
     8521 tgccggtcct accggggtcg ggaaaacaga ggtgacggta cagctttcga aagcgttggg
     8581 cattgagctt ctgcgctttg atatgtccga gtatatggaa cgccataccg tcagccgtct
     8641 gattggtgcg cctccgggat acgttggttt tgatcagggc ggtttgctga ccgatgcggt
     8701 aatcaagcat ccacatgcgg tgctgctgct ggacgaaatc gagaaagcgc atccggacgt
     8761 gttcaatatt ctgttgcagg tgatggacaa cggtacgctg accgataaca acggacgcaa
     8821 agcggacttc cgtaacgtgg tgctggtgat gaccactaac gccggggtac gggaaactga
     8881 gcgcaaatcc attggtctta tccaccagga taacagcacc gatgcgatgg aagagatcaa
     8941 gaagatcttt acaccggaat tccgtaaccg tctcgacaac attatctggt tcgatcatct
     9001 gtcaactgac gtgatccatc aggtggtgga taaattcatc gtcgagttgc aggttcagct
     9061 ggatcagaaa ggtgtttctc tggaagtgag ccaggaagcg cgtaactggc tggccgagaa
     9121 aggttacgac cgggcaatgg gcgcacgtcc gatggcgcgt gtcatccagg acaacctgaa
     9181 aaaaccgctc gccaacgaac tgctgtttgg ttcgctggtg gacggcggtc aggtgacggt
     9241 tgcgctggat aaagagaaaa atgagctgac ttacggattc cagagtgcac aaaagcacaa
     9301 ggcggaagca gcgcattaat ctgattgtca ggtaggttgg tgaagtccgt aatctcgtca
     9361 ggggttacgg actttttatt tatggggtgg aacggtgaaa accctatttt tggaggtgaa
     9421 ggtaagttgt tgataattag tgctgctgga aggtaaggat aaaaaagggt gctgcaggag
     9481 aatgggatgg ttttgcttta ttaacaacgg gctaaacgtg tagtatttga gttcactgcc
     9541 gtacaggcag cttagaaatc gacggggtgc ggtaaaacct ttgcgaacgc gttcactgcc
     9601 gtacaggcag cttagaaatt cacaggtaac atactccacc cgcccaccat gttcactgcc
     9661 gtacagacag ataaaatgcg aaaaaaaagc tcgcactttc gtacgagctc ttctttaaat
     9721 atggcggtga gggggggatt cgaacccccg atacgttgcc gtatacacac tttccaggcg
     9781 tgctccttca gccactcgga cacctcacca aattgttttg ctaccaaacc tcatgggtgg
     9841 caacggggcg ctactatagg gagttggagt aaaacggtca agaagaattt taatgataat
     9901 tattgtttgc tcatactgta aacaacttgt gcagtatatc tacatcgaga caagttatgg
     9961 acttatactt ccaaagtact tcatacatat cacaaaataa aaaggccggt taaaccgacc
    10021 ttttactcgt tctttctctt cgcccatcag gcggtaaaac aatcagcgac tacggaagac
    10081 aatgcggcct ttgctcaggt cgtacggggt cagttcaaca gtcactttgt cgcccgtcag
    10141 gatgcggatg tagtttttgc gcattttacc ggagatgtgt gcagtaacca cgtgaccgtt
    10201 ttctaactct acgcggaaca tggtattagg caacgtttca agaacggtac cttgcatttc
    10261 aatattgtct tctttggcca tctaatcctc tggggtatca ctaccgtaat ttgaaccggc
    10321 aagataatgc cgaagttctg taaataagta aagatttgcg cgctaaatcg caacaaacag
    10381 gttcggcaca ttactccgaa aacacacggc taagccgcac caaaagcgca acgtataagg
    10441 gagcggtgag ataaacgatg ggcgttacct gacgcgaaaa attccttatc ggcagcgggg
    10501 taatgagcgt aaccaactct gcgaccgcaa ttataacact ctggggagaa atgtgccgaa
    10561 aacattcatt cttgtggtga aaacaagcac cgtggtaccc agaaattatt cggcaatcgt
    10621 ccgaggcgca tttgattgag ataattaagg taatcccggc ggggaatttc gcaggcacca
    10681 agcgatgctg tgtgatcgtt aaggacctgg cagtcgataa gcttaccgcc atgaccgata
    10741 aattcctcac agaataccag aagcgccgtt ttagacgcat tttccatccg gctgaacatg
    10801 gactcgccac aaaatagcgt tccctgggcc acgccgtaca taccgccgac aagctcatct
    10861 tcacgccaga cttcaatgga gtgggcatga ccgagttcgt gaaggcgatg gtaggcttcg
    10921 accacgccac gcgtgatcca ggttccttct tcgcgatcgc tggcacagcc ttcaatgacc
    10981 tgaccaaaag cgtaattcat cgtgacacga tagggcgagc gtttatgaaa tcgcttcata
    11041 ctacggctga tatgcagtga ttctggccat agcaccgcgc ggggatcggg cgaccaccag
    11101 aggatggggt cgcctggaga aaaccacgga aaaataccac gctggtaagc cattaacagg
    11161 cgcgcagggc taagatcgcc cccaagtgcc agcaggccgt taggctcacg taatgcgcct
    11221 tccggggaag ggaaggctat tgaatggcga gaaagctgaa ccaagcgcat gaccgcaaaa
    11281 ctccacgcaa gtcggatcgt tcaataatag cttacaaacc ctgcttgaac tggtaataac
    11341 gcccctgtct ggcaagcagt tctgcgtgag taccttgctc aataatttgc ccgttgtcca
    11401 tcactattat ttgttggaaa cgagagagtc cgcgaagtcg atgggtgacc attaacaccg
    11461 ttttctcacg catcattttt gcaagcaatt caaggatctg gctttcggtt gtggcatcta
    11521 agccttcggt aggttcatcc agcaacacca gtggcgcatc atgtaacagc gtacgggcga
    11581 tagccagacg gcgcagttca ccaccggaga gctggcgtcc gccttcacct aaccaactgt
    11641 tgagacctgc atcctcgagc agcttctcca gaccaacgcg acgcaagatc tccgacagag
    11701 cctcatcact actgccaggc gaggcgagta acagattatc acgcagcgtg gcgctaaaca
    11761 gatgcactcg ctgaggaaca acgctgatgg tctgtcgtag agccgcttca ttcaggctgg
    11821 ctatggggct atcgttaagc aaaatctcgc cctgttgcgg gtcccatgcg cgggtcagca
    11881 gttgtaacag tgttgatttg ccgcatccgg ttcgcccgag aatcgctata tgttccccgg
    11941 cgtttacctg aagagaaatc cctttaagtg cctgttggga ttgctccgga taagtgaact
    12001 gaacatcccg taatgtcagc gaaacgcgat cggcaacacg agtttgggta tcagggaagg
    12061 tgacctccgg tttttgatcc gttaagtcag agatacgtac ggcagaggca atgacttgcc
    12121 ccagatgctg aaatgcaccc gttactggtg ccagtgcttc aaacgcggct aacgcacaaa
    12181 agacaaacag ggcaattaat gcgccgggtt gagcattgcc gccaacgccg ccagacgcca
    12241 tccacagcat caggatcacc gctaacgcgc caatgagcag cattatcgct tgcgacaatg
    12301 cggtcagttc agattgacgg cgttgcgctt ccagccattg aatttctgta ttctctagtt
    12361 gcgtgcgata acgatcgctg gcaccaaaaa tggtcagctc agcttgccct tgcagccagg
    12421 ccgtcagttg ttggcgatac tgtccgcgaa gatgagtcag attttgcccg gtgctttttc
    12481 ccgcacgata aaacagcggt ggcatcagga aaagcgtcag taacataacg ctgcccagcg
    12541 taaaggcgag ggtgaaatca aggaaactta acccgattgt caccaccata atcaccacaa
    12601 aagcgcccac cagcggcgag ataacgcgca ggtaaagatg atcgagcgta tcaacatccg
    12661 ccaccacgcg gttgagcaat tcgccctgac gatagcgcgc cagtccggca ggggagaggg
    12721 gcaacaattt gctgaaggtg taaatgcgca gatgctgcaa cacgcggaaa gtcgcgtcgt
    12781 gacttaccag acgttcaaaa taacgcccgg cagtacgggt gattgcggca ccacgcacgc
    12841 ccgcagcggg tagcatatag ttgaagctgt acagtccggc aacccccgca accgctgagg
    12901 ccgagaggaa ccagccggaa agtgtcaaca gaccgatact ggcgagcagc gtcacaattg
    12961 ccagcacaat accaagactt aacatccatt tatgacgttt atacagtgcc agatagggta
    13021 gcaaagcgcg catttaaatc tcctcctgac gatgggccag taatgtggcg aatgggccac
    13081 cagccacact taattccgcg taacgtcctt gctcaataat ccggccatcc tgcataaccc
    13141 aaatgacatc ccagtcagca agatcttcta actggtgggt gaccattaac gttgtctggc
    13201 gcagagaggc ggcattcagc gcctccatta cgcgctgttc actgtgagca tcaaggctgg
    13261 cagcgggttc atccaacagt aatagcgaac agggatttag taacgcacgg gccaccgcca
    13321 cgcgctgcgc ctgccccacg gaaaggcggg cagcctggtc gccaacaggt gtatcaacgc
    13381 cttgtggcag gagcggtaga aactcgctga cccaggcgtt atccagcgct gcttgtaatt
    13441 cttgttcgct ggcatcaggt cgcgccagta gtacgttatc ccgcaatttt gctgccggta
    13501 attgtgggtt ttgcccaacc caggagagat gtttacgcca tgattctggt gataaatcac
    13561 gtaattctat cccgttgatt cgtaatgatc cctgatatga gagaaaacca gaaagcgcgt
    13621 tcagcagtga gcttttacct gaaccgctgc ggccaaccaa cactgcacgt tggcctgctg
    13681 gcaaagtaaa gttcagcggt ccggccagcg ttttaccttc cggcgacgtg ataaacagat
    13741 cctcggcctc aatggtcagc ggatcggtca atgctaattc cgcctcaccg cgctgcggat
    13801 gggcgagcgg ggtttccata aacgttttca gactgtcagc tgcaccaaca gcctgggctt
    13861 tagcatgata aaacgtaccg agatcgcgta atggctggaa aaactctggc gcaaggatca
    13921 gggccagaaa acctgcagcc agcgtcaccc cagtatcgta gtgaccaaaa tccagctcgc
    13981 cgagatagga aaaaccaaag tagaccgcca ccagagcaat tgacagcgag gtaaaaaatt
    14041 cgagaatgcc ggaggataaa aacgccagtc gtagcacttc cattgtccgt tggcggaaat
    14101 cttccgaagc agaacgaata ctttcaattt cagcttcacc acgaccaaaa atacgcaatg
    14161 tttccatgcc gcgcaggcga tcgaggaaat gcccacttaa gcgagcaaga gcgagaaagt
    14221 tacgccggtt agcatcggca gcccccattc caaccagcgc cataaacaac ggaattagcg
    14281 gtgctgtgcc cagcagaatg agcgccgcag cccagttaga ggggaagatc gccaccacaa
    14341 tcagcaacgg caccgacact gccagcgcca tttgcggcag atagcgtgca tagtaatcat
    14401 gcatatcgtc aatttgctcg agtaccagcg tcgcccagct ccccgcaggt ttaccctgaa
    14461 tccacgctgg ccctgcttgt tgcagacggt cgagaacctg acggcggatg gcaaagcgga
    14521 tatgctgccc ggcgtgataa cccacccgtt cgcgtaacca gaccacccat gcgcgcaata
    14581 caaaggtcag aaccagtaac gtaaagggaa gcagcagggc ttcacgggga atattctcca
    14641 taatcatatg ttgcagaatt cgcgccatga accaggcctg ggcaatgatc aatatgccgc
    14701 ttacaaagcc cagcagacga gaaatattca gccaacgttg ggagatgacg ctttgctgtt
    14761 ttaaccagcg ggttaactct ttttgacgag atttattcat tgcacgctta gcaggtgagt
    14821 tatcagaatt atttgcagag caatgttaca acggggaaaa aataaaggcg acccatagtc
    14881 gcaaggtgtc gccttcttta cttttgttac tgatttgtaa aattattttg cgtcagctaa
    14941 accatcgagg tagcgttccg catcaagtgc tgccatgcag ccagtaccgg ccgaagtaat
    15001 ggcctggcga taaatgtgat ccatcacgtc gcctgcggca aagacgccag gaatgctggt
    15061 ctgggtggca ttaccatgaa tacccgactg tactttgatg tagccgtttt ccagttccag
    15121 ctgcccttcg aaaatcgcag tattcgggct gtgaccgata gcaacaaaca gaccggcaac
    15181 gtcgagtgac tcgatgttat cgctgttttg cgtatcgcgc agacgaacgc cagtgacacc
    15241 catttgatcg ccggtcactt cttccagcgt acggttggtg tgcagaatga tgttgccgtt
    15301 ctccacttta tccatcagac gcttaataag gattttttcc gcgcggaaac cgtcacggcg
    15361 gtgaatcaga tgcacttccg aagcgatgtt agacagatac agcgcctctt caaccgcggt
    15421 attgccgccg ccgatgaccg caactttctg gttgcgatag aagaaaccgt cgcaggttgc
    15481 acaagcagaa accccacggc ctttaaaggc ttcttcagag ggcaggccga gatagcgtgc
    15541 agaagctccg gtggcaataa tcagcgcgtc gcaagtgtat tcggcgttat cgccattcag
    15601 acggaacgga cggttttgca gatccacctt gttgatatga tcaaaaatga tctcagtttc
    15661 aaacttggtg gcatgttcgt gcatgcgcgc cattaataac ggaccggtca gatcgtttgg
    15721 atcaccaggc cagttttcca cttccgtggt ggtggtcagt tggccgcctt tttccatgcc
    15781 ggtaatcagc acaggttgca ggttggcgcg cgccgcgtag acagcagcgg tgtatcccgc
    15841 cgggcctgaa cccaggataa gcagtttact gtgtttggtc gtgcccatga gatccccata
    15901 gttgttggca gacaatgggc aggattgtag ggaatttaca gacgtaaaaa aagagtatga
    15961 cgattttgtt aacaatttgt gcaatcggca gcatcgataa gcaggtcaaa ttctcccgtc
    16021 attatcacct ctgctactta aatttcccgc tttataagcc gattaaatga tgaataaacg
    16081 cccttgttaa tgaatatctg gcatgttgta ctaaaaatcg atgttttgct ttgacaatcc
    16141 cctagtgttt tgcgaaaaca ttcgaggaag aaaaaaacag tattcttata tgcgcataac
    16201 catgtatgta aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg
    16261 ccattcgtga tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat
    16321 tgcgcgccgt gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg
    16381 atgtttcagg gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat
    16441 agcaagaagc gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg
    16501 caaaaggatg ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg
    16561 ccgtgccttg agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg
    16621 ctgcttaacc cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat
    16681 cgtggcgcac cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt
    16741 caggagtgtc atttagtatc tggtgatttc gactacctgt tgaaaacacg cgtgccggat
    16801 atgtcagctt accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac
    16861 acacggacat acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg
    16921 cgctaacacg gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac
    16981 agcaacagta ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc
    17041 tgtaacctgg agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga
    17101 caaagttaag tagcggccgc cgccttctgg aggcattgct gatccttatt gtcctgtttg
    17161 ccgtctggtt gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa
    17221 cggcctggca tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ttggcagata
    17281 cgctgttctt tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt
    17341 gttggtttgc ctggcgtcat cagtccagcg acgaatacat tgattatttt gccgtttcgc
    17401 tacgcatcat tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg
    17461 ctgacgatat ctggtatttt gcctccggtg gcgtcattgg cagcttacta agcactacgc
    17521 tacaaccact gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg
    17581 gcctgacgtt gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga
    17641 ttttaaacat tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg
    17701 aagatgagta tgaagacgac gaagagtatg aagatgaaaa tcacggcaaa cagcatgaat
    17761 cacgccgtgc ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat
    17821 tcattaatcc gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg
    17881 atgacgaaga gattacctac actgcacgcg gtgtggctgc cgacccggac gacgtcctat
    17941 tttcgggcaa tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg
    18001 cgccaattac cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg
    18061 ctgcgccggt tgaacctgtg actcagacgc cgcctgttgc ctctgttgat gttccacctt
    18121 cgcaacctac agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg
    18181 ctcctgcacc ggaaggttac ccacagcagt cacaatatgc gcagcctgca gtgcaatata
    18241 atgagccgct gcaacaacca gtacagccgc agcagccgta ttatgcacct gcagctgaac
    18301 aacctgcgca acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct
    18361 ggcaagccga agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa
    18421 cttatcagca gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc
    18481 agcctgttgt ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt
    18541 actattttga agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt
    18601 atcaaccgat tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac
    18661 cttctgttgc agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg
    18721 gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca
    18781 gtctggcaaa tggcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc
    18841 cacgaccgaa acgtatccgc gtgccaactc gtcgtgaatt ggcgtcttac ggtattaagc
    18901 tgccctcaca gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtatgatt
    18961 ctggcgatca gtataacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc
    19021 agttcgccca gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa
    19081 acgcagaaga tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gtgcaaactc
    19141 aacaacaacg ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt
    19201 ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ctgtttacgc
    19261 caattgttga acctgtacag cagccgcaac aaccggttgc accacagcag cagtatcagc
    19321 agccacaaca gccggttgcg ccacagccgc agtatcagca gccacaacag caggttgcgc
    19381 cgcagccaca atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc
    19441 cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc
    19501 agccgcagta tcagcagcca caacagccag ttgcgccaca gccgcaggat accctgcttc
    19561 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca caaaccgacg acgccgctgc
    19621 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    19681 ttgaacaaat ggcgcgtctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    19741 tcgttaatta ctctccgggg ccagttatca ctcgctttga acttaacctg gctcctggcg
    19801 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    19861 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata
    19921 aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc
    19981 cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    20041 tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    20101 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    20161 tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcac ctgttaacgg
    20221 aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg
    20281 agcgtcggta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    20341 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    20401 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtc gtgttggttg
    20461 acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagctcgtc
    20521 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    20581 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    20641 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta
    20701 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    20761 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    20821 agtatgttga tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg
    20881 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    20941 aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21001 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    21061 aagtgctggc cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    21121 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    21181 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    21241 atgatgaaaa aaattgctat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    21301 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    21361 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    21421 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    21481 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    21541 acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    21601 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    21661 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    21721 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcagctgaa atcccagcaa
    21781 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    21841 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    21901 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca
    21961 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttgcattc
    22021 tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg
    22081 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg
    22141 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    22201 tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga
    22261 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    22321 actgctttcc cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca
    22381 agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct
    22441 gccagatgat acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt
    22501 aaatacgctg gaaatgatgg cggatatggc cgaagtctat gatagcggta agcgggtcct
    22561 gaagcctgaa ttactgatcg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg
    22621 cgatcgcttt tacgatctaa tttccgcact gcataagtcg gtacgtggta gcgcacccga
    22681 tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    22741 gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca
    22801 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    22861 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    22921 gtttaaagcc gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt
    22981 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    23041 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    23101 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggtg aaaagctcgc
    23161 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    23221 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    23281 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    23341 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    23401 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    23461 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    23521 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    23581 tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg
    23641 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    23701 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc
    23761 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    23821 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    23881 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    23941 tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct
    24001 gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    24061 tgatctgcca attaagatga tcgcccacac cccatgcttc cgttctgaag ccggttcata
    24121 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    24181 gatcgtgcgc ccagaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    24241 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    24301 ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg
    24361 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    24421 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    24481 tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga
    24541 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    24601 ttttctgaat ctaaaaagcg cctttagggc gctttttttg tctccctttg ataccgaaca
    24661 ataattactc ctcacttaca cgtaatacta ctttcgagtg aaaatctacc gatctctttg
    24721 attttcaaat tattctatgt atacaagcct atatagcgag ctgctataga aataattaca
    24781 caatacggtt tgttactgga atcaatcgtg atcaagcttg agtgagccat tatgaaaacg
    24841 aaaatccctg atgcggtatt ggctgctgag gagagtcgcc gtggtttggt aaaaacgaca
    24901 gcgatcggcg gcctggcaat ggccagcagc gcattaacat taccttttag tcggattgcg
    24961 cacgctgtcg atagcgccat tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt
    25021 acagttaact gtggtagtcg ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa
    25081 tatgtcgaaa cggacaatac cggcgatgac aattacgacg gcctacacta ggttcgcgcc
    25141 tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc cggaccgcct gaaatatccg
    25201 atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc
    25261 tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc
    25321 tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg gccgccggga
    25381 aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca ttacggtgac
    25441 tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg ggcagatggt
    25501 aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa taacccaggc
    25561 gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg ccagaaatct
    25621 aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat
    25681 gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct ggcgtacgtc
    25741 atgatcacta aaaacctggt ggatcaggca ttcctcgata aatattgcgt tggctatgac
    25801 gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta tattctgggt
    25861 gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac cggtgttccg
    25921 gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc gtttatcagc
    25981 cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc tatctcgatg
    26041 ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc gcgtgaaggt
    26101 tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca gaccagcatt
    26161 tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc gctgcgtgat
    26221 ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt
    26281 aactgcctga ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct ccaggatgat
    26341 aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct
    26401 gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact ggatgcatcc
    26461 tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt
    26521 aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt
    26581 actgaaggcc gtacctagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg
    26641 attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac
    26701 ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc aaacccactg
    26761 actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat tgccgctacc
    26821 tgggaattgc cagaaggcga tgtgatcgat ccactgccga tctacacgcc gggctttgaa
    26881 agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt ccactataaa
    26941 tctcgcgttc actcaactta cagcaacgtt gatgtgctga aagcggcttg ccgtcaggaa
    27001 atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga taaagtcagg
    27061 atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg aatgatgccg
    27121 ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg tgtcgataag
    27181 ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg
    27241 tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga caacccagta
    27301 tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa
    27361 agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat atgctggcgg
    27421 cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat
    27481 ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca
    27541 taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct gccgttactg
    27601 tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa
    27661 atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg
    27721 cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac atggcgatct
    27781 ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc
    27841 caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc cgaaggaggt
    27901 gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat
    27961 gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag
    28021 aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg ggcattggct
    28081 ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg ctcaaccggg
    28141 taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt gctgtaggcg
    28201 gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt acgctatggc
    28261 tggtggtgac gatggttctt ggcgtcgtct ttgtctggat gatggtgcgt gtgtataaca
    28321 gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggctgg gcgatgcgtc
    28381 tgctgccagc catttctgta ctggcactgg tggtgagtgg cgtgatgtca gtgatgcagg
    28441 gcacagagct ggcaactatt catagttctg tgcagcaggc cgcagcgctg gtgccggact
    28501 atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc ttgtggattg
    28561 caccacagct aaaaggttat cagcctgcgg ttccgctact atccgtctca ttcattctgc
    28621 ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg accgtgggga
    28681 tggccgtcgc aagctaatca gtaacaaccg gggtttcggc cccggttctc ttttatttct
    28741 gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaata tggttagaga
    28801 acaacgtcgc cagtccttca atatcattac gccagtcacg gtgcagctcg caagccacgc
    28861 cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccacgcc gaatgccggg
    28921 taatttcatt aaaggtccct gatgcatcag tcacgacaaa gacatcaaac ccttcttcaa
    28981 tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca ataattaact
    29041 gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag gcgttaatat
    29101 tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct agaactaatg
    29161 ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg aaatacttag
    29221 ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata tcccgtacaa
    29281 gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt ttatcaagac
    29341 gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt ctgaaatcgg
    29401 tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc tgtgaccatg
    29461 aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa acaatcagat
    29521 gagattaatc atctcttcag cattagcgtt atgaatgagt aatataaatc gataataccg
    29581 ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg gcatgatgcg
    29641 catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg gcctgtcatg
    29701 cttttgctgt ctggcctgct tttgttgact ctggcgatcg cggtgttaaa tacactcgtg
    29761 ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt cagctcatcc
    29821 tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa acgcattggc
    29881 tttaaccgca gctattatct gtcctccttc atttttgctg ctggctgtgc cggccttggc
    29941 ctgatgattg gtttctggag ctggttggcg tggcgttttg tcgcgggcgt cggctgtgcc
    30001 atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg taaccgtggg
    30061 cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa cgtttttagg ccagttactg
    30121 gtcagcaaag tctcaaccga gctgatgagc gtattgccgt gggttacagg tttgacattg
    30181 gcagggatct taccgctgtt gtttacgcgt gtcctgaatc agcaggctga aaaccatgat
    30241 tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt gaatggctgc
    30301 attatctcag gaatcgttct gggatctcta tatggcctga tgcctctgta cctcaatcac
    30361 aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag tgcgggtatc
    30421 ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct ggttttgcgt
    30481 gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc ttagccaggc ggcgatggcc
    30541 ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc gatggcctgg
    30601 gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaagcctt actgttgagc
    30661 tataccgtgg gaagtctgct tggcccgtca tttagcgcta tgctaatgca gaatttctcc
    30721 gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct gatgctgctt
    30781 cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc aagcaaagtg
    30841 tgaacttact gtttcacact c
//
LOCUS       DAJTNV010000044        30014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNV010000044 DAJTNV010000000
VERSION     DAJTNV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO199_002041"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO199_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5536077.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO199_002042"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO199_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5536078.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO199_002043"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO199_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5536079.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO199_002044"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO199_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5536080.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO199_002045"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO199_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5536081.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO199_002046"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO199_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5536082.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO199_002047"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO199_002047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5536083.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO199_002048"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO199_002048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5536084.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO199_002049"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO199_002049"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5536085.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO199_002050"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO199_002050"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5536086.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO199_002051"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO199_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5536087.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO199_002052"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO199_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5536088.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO199_002053"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO199_002053"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5536089.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO199_002054"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO199_002054"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5536090.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO199_002055"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO199_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5536091.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO199_002056"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO199_002056"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5536092.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO199_002057"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO199_002057"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5536093.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO199_002058"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO199_002058"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5536094.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO199_002059"
     CDS             21622..22212
                     /locus_tag="OO199_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5536095.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO199_002060"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO199_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5536096.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO199_002061"
     CDS             22684..23379
                     /locus_tag="OO199_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5536097.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO199_002062"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO199_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5536098.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO199_002063"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO199_002063"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5536099.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO199_002064"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO199_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5536100.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO199_002065"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO199_002065"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5536101.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO199_002066"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO199_002066"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5536102.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7823 c         8256 g         6905 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gaga
//
LOCUS       DAJTNV010000045        29789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNV010000045 DAJTNV010000000
VERSION     DAJTNV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            46..840
                     /locus_tag="OO199_002067"
     CDS             46..840
                     /locus_tag="OO199_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5536103.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            862..2271
                     /locus_tag="OO199_002068"
     CDS             862..2271
                     /locus_tag="OO199_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536104.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2249..3028
                     /locus_tag="OO199_002069"
     CDS             2249..3028
                     /locus_tag="OO199_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5536105.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3025..3885
                     /locus_tag="OO199_002070"
     CDS             3025..3885
                     /locus_tag="OO199_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5536106.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4033..4608)
                     /locus_tag="OO199_002071"
                     /pseudo
     CDS             complement(4033..4608)
                     /locus_tag="OO199_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4625..4885)
                     /locus_tag="OO199_002072"
     CDS             complement(4625..4885)
                     /locus_tag="OO199_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5536107.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4876..6147)
                     /locus_tag="OO199_002073"
     CDS             complement(4876..6147)
                     /locus_tag="OO199_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5536108.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO199_002074"
     CDS             complement(6225..6587)
                     /gene="queD"
                     /locus_tag="OO199_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5536109.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6906..8705
                     /gene="cysJ"
                     /locus_tag="OO199_002075"
     CDS             6906..8705
                     /gene="cysJ"
                     /locus_tag="OO199_002075"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5536110.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8705..10417
                     /gene="cysI"
                     /locus_tag="OO199_002076"
     CDS             8705..10417
                     /gene="cysI"
                     /locus_tag="OO199_002076"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5536111.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10491..11225
                     /gene="cysH"
                     /locus_tag="OO199_002077"
     CDS             10491..11225
                     /gene="cysH"
                     /locus_tag="OO199_002077"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5536112.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11489..11641
                     /locus_tag="OO199_002078"
     CDS             11489..11641
                     /locus_tag="OO199_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5536113.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11835..12926
                     /locus_tag="OO199_002079"
                     /pseudo
     CDS             11835..12926
                     /locus_tag="OO199_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO199_002080"
     CDS             complement(13072..14109)
                     /gene="iap"
                     /locus_tag="OO199_002080"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5536114.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14361..15269
                     /gene="cysD"
                     /locus_tag="OO199_002081"
     CDS             14361..15269
                     /gene="cysD"
                     /locus_tag="OO199_002081"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5536115.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15271..16698
                     /gene="cysN"
                     /locus_tag="OO199_002082"
     CDS             15271..16698
                     /gene="cysN"
                     /locus_tag="OO199_002082"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5536116.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16698..17303
                     /gene="cysC"
                     /locus_tag="OO199_002083"
     CDS             16698..17303
                     /gene="cysC"
                     /locus_tag="OO199_002083"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5536117.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17353..17676
                     /locus_tag="OO199_002084"
     CDS             17353..17676
                     /locus_tag="OO199_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5536118.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17870..18181
                     /gene="ftsB"
                     /locus_tag="OO199_002085"
     CDS             17870..18181
                     /gene="ftsB"
                     /locus_tag="OO199_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5536119.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18200..18910
                     /gene="ispD"
                     /locus_tag="OO199_002086"
     CDS             18200..18910
                     /gene="ispD"
                     /locus_tag="OO199_002086"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5536120.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18910..19389
                     /gene="ispF"
                     /locus_tag="OO199_002087"
     CDS             18910..19389
                     /gene="ispF"
                     /locus_tag="OO199_002087"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5536121.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19386..20435
                     /gene="truD"
                     /locus_tag="OO199_002088"
     CDS             19386..20435
                     /gene="truD"
                     /locus_tag="OO199_002088"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5536122.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20416..21177
                     /gene="surE"
                     /locus_tag="OO199_002089"
     CDS             20416..21177
                     /gene="surE"
                     /locus_tag="OO199_002089"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5536123.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21171..21797
                     /gene="pcm"
                     /locus_tag="OO199_002090"
     CDS             21171..21797
                     /gene="pcm"
                     /locus_tag="OO199_002090"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5536124.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21937..23076
                     /gene="nlpD"
                     /locus_tag="OO199_002091"
     CDS             21937..23076
                     /gene="nlpD"
                     /locus_tag="OO199_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5536125.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23139..24131
                     /gene="rpoS"
                     /locus_tag="OO199_002092"
     CDS             23139..24131
                     /gene="rpoS"
                     /locus_tag="OO199_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5536126.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24251..24658)
                     /locus_tag="OO199_002093"
     CDS             complement(24251..24658)
                     /locus_tag="OO199_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5536127.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24805..25398
                     /locus_tag="OO199_002094"
     CDS             24805..25398
                     /locus_tag="OO199_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5536128.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25398..26345
                     /locus_tag="OO199_002095"
                     /pseudo
     CDS             25398..26345
                     /locus_tag="OO199_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26618..27181)
                     /locus_tag="OO199_002096"
     CDS             complement(26618..27181)
                     /locus_tag="OO199_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536129.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27346..27597
                     /locus_tag="OO199_002097"
     CDS             27346..27597
                     /locus_tag="OO199_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5536130.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27599..29695
                     /locus_tag="OO199_002098"
     CDS             27599..29695
                     /locus_tag="OO199_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5536131.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7298 a         7378 c         8057 g         7056 t
ORIGIN      
        1 atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa aatctttaaa
       61 tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa aaccggcttc
      121 aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg tttgtatgac
      181 gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt gctgatcttt
      241 atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc ggaaatcgtt
      301 gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg gtttaacaac
      361 ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa aaccggcaac
      421 atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat ctacgaaagc
      481 gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat gctgggcggt
      541 cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga ctacaacgtt
      601 gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa caaaatcatc
      661 tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat tggtaaacat
      721 cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga agggctgaaa
      781 aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat tgaaaaataa
      841 tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc aggcgagaag
      901 ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt gcgaatggat
      961 gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg cgcacacctg
     1021 accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac gcccgccatg
     1081 caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg tttgtttctt
     1141 ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat cttcaccttc
     1201 agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac gccagagcat
     1261 cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc agtaggtcac
     1321 accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg cgcattcagc
     1381 gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt tatttccgaa
     1441 aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt gttgattacg
     1501 ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg gcgttttgca
     1561 gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg cgatgaagtg
     1621 gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg gcggcgcacg
     1681 gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat ttatacctgg
     1741 ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag cctgatgctt
     1801 aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct gctggcacat
     1861 cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt aatggcctgt
     1921 ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac caccatttcg
     1981 gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga cattcgctcg
     2041 ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag tactggcctg
     2101 ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct agcgacagtg
     2161 ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc cctcccgctg
     2221 gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta aagccgaacc
     2281 ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta atagcggacc
     2341 ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg ccgcgctgct
     2401 gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca tgggggatga
     2461 acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag ctgtattgct
     2521 ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata ttgccgaatg
     2581 gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg aagggcagaa
     2641 tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca cccaggtgga
     2701 gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac atgggttgcg
     2761 ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg aagtggcctt
     2821 gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta tccgtaaaac
     2881 tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag agcaacgacg
     2941 cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc tatggcagga
     3001 ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa tcaggagaat
     3061 gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct ggcgcactgg
     3121 caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt ggaacagtgg
     3181 cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga tgaactgtcg
     3241 acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc gttagatatt
     3301 ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac gctgcaaaca
     3361 gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa aaacgccacg
     3421 ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga ctggctggtt
     3481 agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag acgggtgctg
     3541 gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc agaaaagctg
     3601 ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga tgctgaaaaa
     3661 gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt tggcgcttcc
     3721 ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc gattaatcat
     3781 gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga ctggaaagtg
     3841 gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc tgccggatga
     3901 tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt aagcgtcgca
     3961 tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat tcaattggtt
     4021 acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat tcgtggtgga
     4081 aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc ataccagccc
     4141 gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt tgggcatgca
     4201 gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat caatgttgtg
     4261 aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt ccgctcttgc
     4321 cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca ccagtttcaa
     4381 aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat gaataaaagc
     4441 atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga tattgccgta
     4501 caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg cattatcttt
     4561 aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa attatcctta
     4621 ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc cactgttgta
     4681 gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg cgatagtcaa
     4741 tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg ttaatcaacc
     4801 gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca tcagcaacgc
     4861 gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg ccagatgcca
     4921 caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa caggtttatc
     4981 accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg cccgatacca
     5041 gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat cccatagcag
     5101 gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc ggtgctggca
     5161 ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat ccatgccgcg
     5221 cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca gccagccgtt
     5281 accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat gcgcaccata
     5341 ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt taaagcgagt
     5401 cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc aaacaatccc
     5461 taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt catcacagat
     5521 tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac gttcttcaat
     5581 agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt cgccagcagg
     5641 acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg ccagcaccac
     5701 ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc cgttttcttc
     5761 atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt cggcttcggc
     5821 aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc cgggctgtaa
     5881 gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt gagtgatgcg
     5941 tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg catgggtata
     6001 taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga gtaacaaaac
     6061 ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac cggcaccaat
     6121 aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac acaacaccct
     6181 aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc gccgcgatag
     6241 atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg cacaacgggt
     6301 ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc cagacctgga
     6361 atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc cgctttcagt
     6421 tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat ttccagtcgc
     6481 accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac atgtggtaag
     6541 cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat aatttctcta
     6601 caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt atctaaaacg
     6661 actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat ttgtttttca
     6721 ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt agtcgatttg
     6781 gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta accttacctt
     6841 ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact ggaacataac
     6901 gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg gagcaactgg
     6961 cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg gtttctggct
     7021 atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca gcgcccgccg
     7081 cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg cgccgggttg
     7141 ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg gtgaacgcgg
     7201 gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg acgtcaacgc
     7261 aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg ttctccaaaa
     7321 aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat agctcttatg
     7381 aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg gggggtgaac
     7441 gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc gagtggcgcg
     7501 cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg caatccgtcg
     7561 ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg ccgctggtgg
     7621 ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac gttcgccata
     7681 tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg ctgggcgtct
     7741 ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg ctgacaggcg
     7801 atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta cagtggcact
     7861 tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt acccgcagtg
     7921 aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc gcgacgacgc
     7981 tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg ctaattaatc
     8041 tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg gaagtcgaga
     8101 acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc gcccgtgccg
     8161 gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc cgcgtattta
     8221 tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg attatgattg
     8281 gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc gctgacgaag
     8341 cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac ttcctgtatc
     8401 aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat ctcgcctggt
     8461 cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag ggcgcggagc
     8521 tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct aatcgcatgg
     8581 cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc atggacaccg
     8641 aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag cgagatgtct
     8701 actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga cagacgccga
     8761 gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt taaacgacgg
     8821 tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg gcatgtatca
     8881 gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc cgcgccacgc
     8941 gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt ggcaggcgat
     9001 cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca accgccagac
     9061 gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga tgctgcactc
     9121 ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac tctgcacctc
     9181 gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga agatttctga
     9241 acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg aaaaagtcgc
     9301 caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt tcaaaaccac
     9361 ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga acttcgtggc
     9421 gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg ggctttctat
     9481 cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct atctgccgct
     9541 agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact ggggtaaccg
     9601 aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg ttgagacgtt
     9661 taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc cgtatgaatt
     9721 tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact ggcacctgac
     9781 gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga aaactggcct
     9841 gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc agaatctgat
     9901 catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca aagagagcgg
     9961 gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt cattcccgac
    10021 ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg acaacatcga
    10081 taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg taacaggctg
    10141 cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta aagcaccggg
    10201 tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac ggatgtataa
    10261 agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag ggcgctgggc
    10321 gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg gcatcattcg
    10381 cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt cttgtaggcc
    10441 tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct atgtccaaac
    10501 tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg ctggcagaaa
    10561 ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg ctggataatc
    10621 tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg agcctgcatc
    10681 tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc tatctgttcc
    10741 cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac ctgaaagtgt
    10801 accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg tgggaacagg
    10861 gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg aaccgggcgc
    10921 tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa tccggcagcc
    10981 gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg ccgattatcg
    11041 actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa tatcacccat
    11101 tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa tgggaacccg
    11161 gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga ttacacgaag
    11221 ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc gtttttattt
    11281 tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac aggtatagaa
    11341 tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca gatggacaaa
    11401 agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca attcggatgt
    11461 tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg tggcaatcat
    11521 cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt tatgtgagtt
    11581 gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat cgaagaagta
    11641 gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc tcctgcaccc
    11701 tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat tatgaatttc
    11761 aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata tatatcatag
    11821 atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa aatattgtga
    11881 ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc gacgactatc
    11941 accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg gttaaaagaa
    12001 attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag gaacaggcag
    12061 ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc cgctcttttc
    12121 aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa aattacgaaa
    12181 aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt gaatgtcagg
    12241 agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata gaaatgtgga
    12301 tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa ttggataatt
    12361 ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag ccactgtttc
    12421 cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt ataaaacaac
    12481 tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg tcaacgcgat
    12541 tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag gctttagttc
    12601 aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct ttcaccggaa
    12661 ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag gtgctggatc
    12721 tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc ggcgcaggta
    12781 aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac gctcaggggc
    12841 tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga ctggtcaaaa
    12901 cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata aaccggcgca
    12961 atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat gggtatcact
    13021 tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc atcaagacgc
    13081 cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc agcgacgttc
    13141 tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta cgtcatgcca
    13201 gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat ccttatttcc
    13261 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
    13321 tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
    13381 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
    13441 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
    13501 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
    13561 ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
    13621 ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac ctgcggcgtt
    13681 atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag catcgctcag
    13741 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
    13801 agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat cgcgggcggt
    13861 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
    13921 ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc gtcccggaaa
    13981 gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag gtgacgaggc
    14041 gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt ggcgcaatgc
    14101 ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata gtatgaaact
    14161 gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt gccttaagca
    14221 ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca ttctaattgg
    14281 taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc
    14341 ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg caactggagg
    14401 cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg gtgatgctct
    14461 actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg ttttatccag
    14521 gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc gagatgtatg
    14581 agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat aaaaacccgg
    14641 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta
    14701 tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg
    14761 gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct ttccgtgacc
    14821 gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac tacaacgggc
    14881 aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc gagcaggata
    14941 tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc gctgcggaac
    15001 gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
    15061 aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc
    15121 tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg
    15181 tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg ggatccatgg
    15241 agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca acaaatcgcc
    15301 aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct gctgcgtttt
    15361 ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct gctgcacgat
    15421 acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa gcgtcacggc
    15481 acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc tgagcgcgaa
    15541 cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg taaatttatt
    15601 atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg cgcatcgaca
    15661 tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca aacccgtcgt
    15721 cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat caacaaaatg
    15781 gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta tctgaccttt
    15841 gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc actggaaggt
    15901 gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac actgctcgaa
    15961 gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg cttcccggtg
    16021 cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct ggcatccggt
    16081 cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc aaacgtcgcg
    16141 cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc gatcaccctg
    16201 gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc agacgaagcg
    16261 ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca gccgctttcc
    16321 ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg tgttgatggc
    16381 attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa cctgccgctg
    16441 aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga tcgctatcag
    16501 caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt gaccgtgggt
    16561 gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga attcagtgca
    16621 ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg cgcgcgcgat
    16681 ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat agtcatccgg
    16741 tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg tggtttaccg
    16801 gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg ttacataaac
    16861 tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta tgcagcgatc
    16921 tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa gtggcgaatt
    16981 tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac cgcgccgaac
    17041 gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt gtcgatacgc
    17101 cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg cgtgccggtg
    17161 aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg gcagaaattc
    17221 atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat ctgttgagac
    17281 agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt cgtcaaagta
    17341 acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga cagccttact
    17401 gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat ctcctggtta
    17461 tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt ctttctctac
    17521 acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat tgcgctgcat
    17581 tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac agtaggcatt
    17641 atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg tctcctctgt
    17701 tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa
    17761 caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag
    17821 agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga tgggtaaact
    17881 aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa
    17941 cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag ctacaaacgc
    18001 gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca atggcggcca
    18061 ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacttt
    18121 ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa acaatcgata
    18181 aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc
    18241 ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa
    18301 tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg tgacacgtgt
    18361 cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg cgaatcatcc
    18421 gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg caggtctgaa
    18481 agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt gtttgcatca
    18541 ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag ggatcctcgc
    18601 cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac
    18661 cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt
    18721 acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg aagcctcggc
    18781 gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata acattaaagt
    18841 cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca tccatcagga
    18901 gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca
    18961 attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca ttctgatggc
    19021 gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggggatatc
    19081 ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgcta
    19141 cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt cgatgtcact
    19201 atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc
    19261 gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactt
    19321 ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca
    19381 acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc
    19441 tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg
    19501 gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg
    19561 cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc gctgggcaaa
    19621 aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc aaggaaatgc
    19681 ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc
    19741 gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg cgcgaagtga
    19801 gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact
    19861 acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgctggg
    19921 cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc
    19981 gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag
    20041 ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta gcaaccaccg
    20101 aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat
    20161 tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg
    20221 tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa gccgcgcgca
    20281 gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac gtcaccgtag
    20341 agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg gaacttatca
    20401 acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca tgcacccggt
    20461 atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat
    20521 cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc
    20581 tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt ctatcttggc
    20641 gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg
    20701 aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat
    20761 ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg
    20821 gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt
    20881 cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg cgtgacgcgc
    20941 tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg
    21001 ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct
    21061 gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac tgcgcatagc
    21121 gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca
    21181 gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat gagcaggtgc
    21241 tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag
    21301 cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg
    21361 tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg gaaattggca
    21421 ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg
    21481 aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt gatttacata
    21541 atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg ccgtttgacg
    21601 ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg
    21661 aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc
    21721 gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga
    21781 agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgta
    21841 ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca cggtaattat
    21901 gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc
    21961 accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac
    22021 acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaatactaat
    22081 tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc gcagcaaccg
    22141 caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca
    22201 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    22261 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    22321 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    22381 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    22441 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    22501 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    22561 tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact
    22621 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc
    22681 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    22741 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    22801 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa cgcgctgcgc
    22861 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    22921 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    22981 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    23041 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    23101 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    23161 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    23221 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    23281 agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    23341 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    23401 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    23461 gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac ttggggctga
    23521 tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct
    23581 ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact attcgtttgc
    23641 cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag ttgtcccata
    23701 agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg
    23761 acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg
    23821 gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag
    23881 ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg
    23941 ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa gcggcaacac
    24001 tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg
    24061 aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt
    24121 tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct
    24181 tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt cctacccggc
    24241 aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc tcctgctctt
    24301 ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt atcgccgcag
    24361 caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg tcagcgacgt
    24421 catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct gctagcgttg
    24481 ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt ttatcagcaa
    24541 tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc tgccagcgag
    24601 ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt aacgccatgt
    24661 aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga agaagatgtt
    24721 gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat aacgttcgta
    24781 tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg ggctaccggt
    24841 gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt cgagactcat
    24901 ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta cagcgctcgc
    24961 gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc gatcatctca
    25021 tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa aacgctcgcc
    25081 ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt cgtgctcaaa
    25141 gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat ccatctcgaa
    25201 aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc tgccttttat
    25261 aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt gctggatcaa
    25321 tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc ccggaatttt
    25381 tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag gcgcttgatg
    25441 accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat ctggcagcag
    25501 cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt gataatattc
    25561 gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag aaccacgcga
    25621 tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag tttatccgcc
    25681 gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg gcgcagaaca
    25741 ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc ctgaatgatg
    25801 gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg ctcgacccgg
    25861 ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc aagcgtaagc
    25921 tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa gcagaagagc
    25981 gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc acgctgatgg
    26041 gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg ctgcgtgaaa
    26101 gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg ggttcagaag
    26161 tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg ttcggtgagt
    26221 ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat aaagtctctt
    26281 atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc gaaatcgact
    26341 acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
    26401 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
    26461 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
    26521 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
    26581 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
    26641 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
    26701 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
    26761 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
    26821 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
    26881 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
    26941 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
    27001 gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
    27061 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
    27121 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
    27181 ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa taaaccataa
    27241 aaacaccaaa gaacccttta catggaatct ttggtatctt aatctttatc catacggata
    27301 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
    27361 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
    27421 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
    27481 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
    27541 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
    27601 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
    27661 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
    27721 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
    27781 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
    27841 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
    27901 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gctgggtatc
    27961 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaaccccgg
    28021 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
    28081 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
    28141 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
    28201 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
    28261 tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag cctacgagga
    28321 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
    28381 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga aacaaaaagg
    28441 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
    28501 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
    28561 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
    28621 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
    28681 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
    28741 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
    28801 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
    28861 gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg gtgctgaccg
    28921 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
    28981 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
    29041 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
    29101 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gccaggcaga
    29161 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
    29221 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
    29281 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
    29341 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
    29401 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
    29461 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
    29521 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
    29581 ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac gcgatttccg
    29641 gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg cctgaataaa
    29701 aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt gaaataaccg
    29761 aggaaatact atgactgata ttgatgatg
//
LOCUS       DAJTNV010000046        29636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNV010000046 DAJTNV010000000
VERSION     DAJTNV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..186)
                     /locus_tag="OO199_002099"
     CDS             complement(<1..186)
                     /locus_tag="OO199_002099"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5536132.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO199_002100"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO199_002100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5536133.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="OO199_002101"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="OO199_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5536134.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="OO199_002102"
     CDS             3863..4144
                     /locus_tag="OO199_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5536135.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4437..4961
                     /locus_tag="OO199_002103"
     CDS             4437..4961
                     /locus_tag="OO199_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5536136.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5042..5716
                     /locus_tag="OO199_002104"
     CDS             5042..5716
                     /locus_tag="OO199_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5536137.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5732..8212
                     /locus_tag="OO199_002105"
                     /pseudo
     CDS             5732..8212
                     /locus_tag="OO199_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8228..9261
                     /locus_tag="OO199_002106"
                     /pseudo
     CDS             8228..9261
                     /locus_tag="OO199_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="OO199_002107"
     CDS             complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="OO199_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5536138.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="OO199_002108"
                     /pseudo
     CDS             complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="OO199_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10870..11142
                     /gene="rcnR"
                     /locus_tag="OO199_002109"
     CDS             10870..11142
                     /gene="rcnR"
                     /locus_tag="OO199_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5536139.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11234..11330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11365..12153
                     /gene="thiM"
                     /locus_tag="OO199_002110"
     CDS             11365..12153
                     /gene="thiM"
                     /locus_tag="OO199_002110"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5536140.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12150..12950
                     /gene="thiD"
                     /locus_tag="OO199_002111"
     CDS             12150..12950
                     /gene="thiD"
                     /locus_tag="OO199_002111"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5536141.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13015..13836
                     /locus_tag="OO199_002112"
     CDS             13015..13836
                     /locus_tag="OO199_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5536142.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13884..14630
                     /locus_tag="OO199_002113"
     CDS             13884..14630
                     /locus_tag="OO199_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5536143.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14604..15569)
                     /locus_tag="OO199_002114"
     CDS             complement(14604..15569)
                     /locus_tag="OO199_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5536144.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15566..16569)
                     /locus_tag="OO199_002115"
                     /pseudo
     CDS             complement(15566..16569)
                     /locus_tag="OO199_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16566..17843)
                     /locus_tag="OO199_002116"
     CDS             complement(16566..17843)
                     /locus_tag="OO199_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536145.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18100..19152
                     /gene="fbaB"
                     /locus_tag="OO199_002117"
     CDS             18100..19152
                     /gene="fbaB"
                     /locus_tag="OO199_002117"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5536146.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19460..20314
                     /gene="gatY"
                     /locus_tag="OO199_002118"
     CDS             19460..20314
                     /gene="gatY"
                     /locus_tag="OO199_002118"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5536147.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20343..21605
                     /gene="gatZ"
                     /locus_tag="OO199_002119"
     CDS             20343..21605
                     /gene="gatZ"
                     /locus_tag="OO199_002119"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5536148.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21615..22067
                     /gene="gatA"
                     /locus_tag="OO199_002120"
     CDS             21615..22067
                     /gene="gatA"
                     /locus_tag="OO199_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5536149.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22098..22382
                     /gene="gatB"
                     /locus_tag="OO199_002121"
     CDS             22098..22382
                     /gene="gatB"
                     /locus_tag="OO199_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5536150.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22386..23741
                     /locus_tag="OO199_002122"
     CDS             22386..23741
                     /locus_tag="OO199_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5536151.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23789..24829
                     /gene="gatD"
                     /locus_tag="OO199_002123"
     CDS             23789..24829
                     /gene="gatD"
                     /locus_tag="OO199_002123"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5536152.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24935..25708
                     /locus_tag="OO199_002124"
     CDS             24935..25708
                     /locus_tag="OO199_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5536153.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="OO199_002125"
     CDS             complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="OO199_002125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5536154.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27095..27412
                     /locus_tag="OO199_002126"
     CDS             27095..27412
                     /locus_tag="OO199_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536155.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="OO199_002127"
     CDS             complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="OO199_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5536156.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29262..29593)
                     /locus_tag="OO199_002128"
                     /pseudo
     CDS             complement(29262..29593)
                     /locus_tag="OO199_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7702 a         6877 c         7478 g         7579 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4201 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4261 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4321 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4381 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4441 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4501 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4561 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4621 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4681 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     4741 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     4801 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     4861 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     4921 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     4981 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5041 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5101 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5161 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5221 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5281 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5341 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5401 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5461 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5521 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5581 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5641 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     5701 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     5761 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     5821 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     5881 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     5941 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6001 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6061 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6121 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6181 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6241 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6301 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6361 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6421 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6481 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6541 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6601 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6661 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     6721 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     6781 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     6841 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     6901 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     6961 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7021 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7081 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7141 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7201 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7261 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7321 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7381 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7441 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7501 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7561 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7621 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7681 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     7741 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     7801 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     7861 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     7921 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     7981 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8041 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8101 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8161 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8221 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8281 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8341 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8401 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8461 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8521 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8581 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8641 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     8701 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     8761 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     8821 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     8881 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     8941 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9001 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9061 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9121 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9181 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9241 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9301 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9361 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9421 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9481 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9541 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9601 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9661 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
     9721 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
     9781 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
     9841 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
     9901 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
     9961 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10021 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10081 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10141 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10201 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10261 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10321 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10381 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10441 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10501 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10561 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10621 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10681 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    10741 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    10801 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    10861 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    10921 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    10981 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11041 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11101 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11161 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11221 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11281 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11341 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11401 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11461 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11521 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11581 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11641 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    11701 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    11761 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    11821 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    11881 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    11941 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12001 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12061 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12121 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12181 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12241 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12301 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12361 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12421 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12481 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12541 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12601 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12661 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    12721 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    12781 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    12841 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    12901 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    12961 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13021 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13081 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13141 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13201 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13261 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13321 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13381 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13441 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13501 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13561 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13621 accaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ttatcaacgt
    13681 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
    13741 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggggat gagttgcagg
    13801 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
    13861 actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
    13921 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
    13981 aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt gagcgtgatt
    14041 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
    14101 aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
    14161 actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgtgcggg aaaaagcccg
    14221 atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg
    14281 cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
    14341 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
    14401 gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
    14461 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
    14521 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    14581 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    14641 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    14701 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    14761 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    14821 gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca
    14881 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    14941 gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc catgatccgc
    15001 gccagtaatg cgtccgggat atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg
    15061 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    15121 ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca ttggcgattc
    15181 cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt
    15241 gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa cccctctttc
    15301 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    15361 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    15421 acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    15481 atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc
    15541 agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    15601 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc gcctttaacg
    15661 caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta
    15721 tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt gggtcggttt
    15781 gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg gtgcctgcgc
    15841 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta cgcacaattt
    15901 tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct
    15961 gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa
    16021 tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat
    16081 gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca cgcgctggaa
    16141 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca
    16201 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    16261 gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta cgcccaatca
    16321 gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac aggccatcga
    16381 ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc
    16441 aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc
    16501 ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc
    16561 tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac cttgatagcc
    16621 gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc
    16681 atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt taccggttcc
    16741 tgataagcga acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg
    16801 aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg cacgggggct
    16861 tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc
    16921 aaaataccga ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag
    16981 aagccatagc agatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag
    17041 cgtttagtga aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc
    17101 atccagccgg tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa
    17161 tagaacgcca gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta
    17221 tcgcgcagca ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg
    17281 ctttttggcg gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agaacttccg
    17341 gcggtaatca gcagcgggat gttagtcggt gagatatcgg cataccccag catttgcggc
    17401 aagaaaccac atgccagacc ggaggcaatc cagccgatag tgcccatcac acgaatgcgt
    17461 gggaaatcac gctccacatc cggcacgttg gcaaaagcga tgctgttagt cagcgcaatg
    17521 gtcggcatat aggttagcga gtaggccagc agtaacggaa agaacccggc aaaagtggtc
    17581 tgttgcgcag cgaaatacat cagcacagca ccagcgaaca tcaataccgc cagcactttc
    17641 tgcgccgaga aaaagcggtc agtgatggag ccaaccagaa ttggcgacag gatcgccgca
    17701 atggctgtgc aggcatacga ccagccaatt tctccggcac taaaaccgct tttacttaac
    17761 cacaaccaca atggcacaaa ccacgcgccc cagataaacc attcaacaaa catcatgaac
    17821 gacagctttg ctgttgtttt catcttttaa ttccttcatg acaggtaatg taaggtacgc
    17881 gatgacaata ccacataata atgccttttg aatacctttg cggtgaaaac ttgaccgcgc
    17941 taacattttt tctgatgaat cgagccaaca gaaaacgctg aaaaaacatc caaaagatgg
    18001 aaaaactcgc ccgtaacgac taggattatg attgcccgca aaaggcgggc caggacaatc
    18061 catagccgat atccaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    18121 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    18181 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    18241 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    18301 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    18361 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    18421 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    18481 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    18541 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    18601 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    18661 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    18721 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    18781 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    18841 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    18901 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    18961 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    19021 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    19081 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    19141 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    19201 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    19261 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    19321 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    19381 ttgtgatcat tatttcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    19441 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    19501 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    19561 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    19621 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    19681 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    19741 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    19801 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    19861 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    19921 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    19981 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    20041 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    20101 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    20161 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    20221 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    20281 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    20341 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    20401 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    20461 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    20521 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    20581 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    20641 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    20701 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    20761 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    20821 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    20881 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    20941 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    21001 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    21061 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    21121 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    21181 tcggtcccgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    21241 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    21301 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    21361 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    21421 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    21481 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    21541 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    21601 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    21661 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    21721 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    21781 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    21841 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    21901 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    21961 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    22021 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    22081 aataaaaagt actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    22141 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    22201 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    22261 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    22321 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacaggggt
    22381 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    22441 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    22501 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    22561 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    22621 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    22681 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    22741 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    22801 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    22861 agctctgcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    22921 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    22981 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    23041 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    23101 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    23161 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    23221 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    23281 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    23341 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    23401 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    23461 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    23521 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    23581 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    23641 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    23701 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    23761 ttatccccct taacaatcag gagtttttat gaaatcagta gtgaatgata ctgatggtat
    23821 cgtgcgcgtt gcaaaaagcg tcattcctga aattaaacat caggatgagg tgcgggtaaa
    23881 aattgccagc tcgggcttat gtggttccga tttacccagg atatttaaaa atggtgcaca
    23941 ttattatcca ataacgttag gccatgaatt tagcggctat attgatgcgg tgggatccgg
    24001 tgttgatgat ttacaccctg gcgatgcggt tgcctgtgtg ccgttattac cctgttttac
    24061 ttgtccagag tgtttgaaag ggttttattc ccagtgcgca aaatatgatt ttattggctc
    24121 gcggcgtgat ggtggatttg ctgaatatat tgtcgttaag cgaaaaaatg tctttgctct
    24181 acccacggat atgcctattg aggatggggc ttttattgag ccgattaccg ttggcctgca
    24241 tgcttttcat ttagcgcaag gttgtgagaa taaaaacgtt attattattg gtgccggaac
    24301 cattggcctg ctggctattc agtgcgctgt cgcgctggga gcaaagagtg tgacggcgat
    24361 cgacattagc tcagaaaaac tggcactggc aaaatctttc ggtgcgatgc aaacatttaa
    24421 cagtagcgaa atgagcgcgc cgcaaatgca gagcgtttta cgcgaactgc gctttaatca
    24481 gcttatcctc gagacggctg gcgtaccgca aactgtcgaa ctggcggtag agattgccgg
    24541 tcctcatgcc caactggcgc tggtgggcac gttgcatcag gatctgcatt taacatcggc
    24601 aacgtttggc aaaatattgc gtaaagagct gacggttatc ggcagttgga tgaactattc
    24661 cagcccttgg ccggggcagg agtgggaaac ggcgagccgg ttgctgacag aacgtaagtt
    24721 aagcctggag ccattaatcg ctcaccgtgg aagctttgaa agcttcaccc aggcggtgcg
    24781 tgacatcgct cgtaatgcca tgccgggcaa agtgttgctc attccatgaa accgcgggcc
    24841 agcgtgatgc tggcccggta ttgtgcaaaa cagatcattc accaatggtc ccccttcgtt
    24901 tacactagcc acaattgaat atgggtaaat gacgatgaat tcattcgagc gaaggaataa
    24961 gatcatccaa ttagtgaatg aacagggaac cgtgcttgtt caggatctgg cgggagtatt
    25021 tgctgcctcg gaagcgacaa tccgtgccga tttgcgcttt ctcgaacaaa aaggcgtggt
    25081 tacgcgcttt catggcggtg cggcgaaaat aatgtccggt aatagtgaaa ccgagaccca
    25141 ggaagtcggg tttaaagagc gatttcagct cgccagcgcg ccaaaaaaca gaatagcgca
    25201 ggcggcagtc aaaatgatcc atgaagggat gaccgttatt ctcgacagcg gaagtaccac
    25261 aatgcttatc gccgaaggat taatgaccgc taaaaatatc acggtgatta ccaacagtct
    25321 cccggcagcg tttgcccttt ccgaaaataa agacattact ctggtcgtct gtggtggcac
    25381 ggtccgccat aaaacgcgct cgatgcatgg ttccattgcc gagcgttcat tgcaggatat
    25441 taatgccgat ttaatgtttg tcggtgctga tggtattgat gcggttaatg gtattacgac
    25501 ctttaatgaa ggttattcga ttagcggggc gatggtcacc gccgcaaata aagtgattgc
    25561 tgttctcgat tcatcgaaat ttaatcgtcg cggttttaat caggtactgc caatcgaaaa
    25621 aattgacatt attgttaccg atgatgctgt gtcagaggtg gataaattgg cattgcagaa
    25681 gacacgggta aaattaatta cggtgtagta ttgttctgta tgccggataa ggtgtttaca
    25741 ccgcatccgg cattacggat tctccctggt tcaagagaga atagtttgat taacgcaata
    25801 atggacaatc tggtggtaat cgacaacgca acgccgccgg aagtatttca atatggaagt
    25861 tttgcccact caacggttcg ccatcaagat taaaagtgat ttcgtgtggg gcttgtatat
    25921 caaaccacga cgaagcgcct tcgataatat tcgggttatc ttcgtcagat tttaaggttg
    25981 atacgagagc cggaagaatt tcatcgccgg taaaaatgcg cagttgcagc aagccatcgt
    26041 taattaacgc gttcgggcac aattgttgac cgccaccggc ctgacgcccg ttaccaatac
    26101 caatgaccag ggcgtcacct tgccagtgaa agttttcacc gcggatttca caacggtccg
    26161 gttgcagagt atccatgcgc attaagccat gaatgatgta agagacgcca cccagcgcgg
    26221 cttttaattt ttccggcgtt tctgtggtaa tacgcgtccc aaatccgcct gtcgccatat
    26281 taataaaaca ggtttgtttg ttgacctgcg ccatatctat cgcaatggca ttaccggcaa
    26341 ttgccagttt cagcgcctta tccagtgcct caggaatccc tacactggtg gcaaaatcat
    26401 tggcggttcc taatggcaaa attcccagcg cgggtatgtc atccccctca cactgaatca
    26461 acgccgtaga aacttcatta atggtgccat cgccaccacc ggcaatcacc gttgcgacgc
    26521 ccaacttccg ggcctcctct acaaatcgtg cggcatcacc tttctcccag gtgacccgca
    26581 catggatcgt cattccttcc tcacgcaaca gcataattgc ttcgcgcaag ggtagattgt
    26641 cagtactttt accattaaga atcagtaagc tggcgggaaa ttctgccatg atcgtatgtg
    26701 cctttatgat tggtctgtag atagtgtaga gcagaaaaca aaaggtggag tcagaacagg
    26761 atgaaagtcg gaggataaaa agtcagttca agtatggagg attcagtggt taccaccagg
    26821 agcttgcaat ggggacagcg aatactaacg gctgccaccg ataaatttca aaaaagagca
    26881 tatacctaat attcaactaa acagtggcat cttcaatata atatattaaa gcccccatgg
    26941 agttaccctg aagggcctca atgtccgtaa ttcctactta tgtaggaaat gttgtacaga
    27001 gcatttatta taatcctatt caattataat aatcatgcca ttattatatt taaacactag
    27061 agagtgtcgt tggtatttaa tgggggaagg tgagatgaaa aagatagctg ctatatcatt
    27121 aattagtatt tttcttatat ctggttgtgc tgtgcataat gatgaaacaa gtatcggtaa
    27181 atttggtctt gcatataaaa gtaatattca gcgtaaactc gataaccaat actacaccga
    27241 agccgaagct tctttagcca ggggcagaat atctggtgca gaaaatatag taaaaaatga
    27301 tgcagcccat ttctgtgtta ctcagggcaa aaaaatgcag atagttgacc tgaagacaga
    27361 aggtgcagga ttacatggcg tcgctcgtct gacattcaaa tgtggagagt gagaatattt
    27421 ttttgtaagc gtcaaacatg cgcgttctgg ctgtgcgtag ccggaacctg ggcgagcacg
    27481 ataccgttac gtgaaaggca ttgtgctatg aagggggatt ctatcgatgt ggtcaatgga
    27541 agacggttat cagggatcaa tccttcttta cagcttatgt acaaaaaata agcccgtgta
    27601 agggagatta cacaggctaa ggaggtggtt cctggtacag ctagcatttt atggattatg
    27661 tttttcagcg aaacggatga taaccttaat aaatgcagct gtatgtgatc ggtttctaag
    27721 aattttccat ccgggaaaaa taatcgaaat taatcactta ccgtgggggt tacgcgtggt
    27781 ttccccggag aaattacgca ttagcagcgc gtaatttagc tcaagatcct gcgggaccgg
    27841 gagccacaca gtataaccat cgcctggtgc gaccggcata gcttcgcctt tggcgttttc
    27901 catgtgctca agggtaaagt taatgttgcc ttgcggcgtc atcagctcaa gactgtcgcc
    27961 aacggagaat ttatttttca ccgctaccgc tgcgaggtcc cccttgcgct caccggtaaa
    28021 ctcaccaaca aactgctggc ggtcagaaac tgaataaccg tattcgtagt tctgataatc
    28081 gtcgtgagta tgacgacgca ggaaaccttc ggtatagcca cgatgcgcca gaccttccag
    28141 agtttccagc aggctggtat cgaacggttt tcccgcagcg gcgtcatcga tagctttgcg
    28201 gtaaacctgt gcggtgcgtg cgcaatagta gaaagatttg gtacgacctt cgattttcag
    28261 cgaatgcacg cccattttgg tcaggcgttc tacatgggcg atggcgcgca gatctttcga
    28321 gttcatgatg taagtgccgt gctcatcttc aaacgcggtc atatactcgc ccggacgctg
    28381 ggcttcttcg atcataaaca ctttgtcggt tggcgcgccg atacccagcg tcggctcaac
    28441 attttgcacc ggaatcggct cgtacttgtg tacgatgttg ccaacgtcat cttctttccc
    28501 ttcctggacg ttgtactccc agcggcaggc gttggtgcag gtgccctggt tcgggtcgcg
    28561 cttgttgata tagccagaga gcaggcagcg accggagtag gccatgcaca gtgcgccgtg
    28621 aacgaagatc tcgatctcca tatccggcac ctgattatgg atctcttcaa tctcttccag
    28681 cgacagctcg cgagagagga tcacgcgggt cagtcccatt tgctgccaga atttcaccgt
    28741 cgcccagttc acggcgttag cctgcaccga gaggtggatc ggcatttcag ggaagtgctc
    28801 acgcaccagc ataatcagcc ctggatcgga cataatcagc gcatccggcc ccatttccac
    28861 caccggtttc aggtcacgga taaaggtttt cagcttggcg ttgtgcggtg caatgttgac
    28921 cacgacataa aactttttcc ccagcgcgtg ggcttcattg atgccgagct gaagattttc
    28981 gtggttgaat tcgttgttgc gcacacgtag ggagtaacgc ggctggcccg cataaacagc
    29041 atctgcgcca taagcgaaag cgtaacgcat atttttcagc gttcccgccg gggaaaggag
    29101 ttccggttta aacataattc tctcgttctg atgacaggtc agatccgctt cacctgatga
    29161 agcggttagg ggaggccccc actttaaggg cgggcattgt agcgctgtag gggggatctg
    29221 gtaaagcgcc ggacgccaac aacccacgcc cggcatcgta attacgtatt gtctttcacc
    29281 gtctggcttg tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg
    29341 gcgtacatct ggctggagcc gataatttga tgattagcgg ctttcagatt gaaatagaat
    29401 ttgccgttgc ttgccgtttt ttctcatagc gctcttcttg cgggctgttg ctacgcaccg
    29461 acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg aggatagtct
    29521 caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa
    29581 accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc atctaa
//
LOCUS       DAJTNV010000047        29236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNV010000047 DAJTNV010000000
VERSION     DAJTNV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..218
                     /locus_tag="OO199_002129"
     CDS             <1..218
                     /locus_tag="OO199_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5536157.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO199_002130"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OO199_002130"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5536158.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO199_002131"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OO199_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5536159.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="OO199_002132"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="OO199_002132"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5536160.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="OO199_002133"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="OO199_002133"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5536161.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="OO199_002134"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="OO199_002134"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5536162.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="OO199_002135"
     CDS             7229..8179
                     /locus_tag="OO199_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5536163.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="OO199_002136"
     CDS             complement(8220..8690)
                     /locus_tag="OO199_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5536164.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="OO199_002137"
     CDS             complement(8745..9947)
                     /locus_tag="OO199_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5536165.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="OO199_002138"
     CDS             complement(10034..10576)
                     /locus_tag="OO199_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536166.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="OO199_002139"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="OO199_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5536167.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="OO199_002140"
     CDS             complement(11319..11921)
                     /locus_tag="OO199_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5536168.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="OO199_002141"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="OO199_002141"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5536169.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="OO199_002142"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="OO199_002142"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5536170.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="OO199_002143"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="OO199_002143"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5536171.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="OO199_002144"
     CDS             16319..17209
                     /locus_tag="OO199_002144"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5536172.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="OO199_002145"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="OO199_002145"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5536173.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="OO199_002146"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="OO199_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5536174.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="OO199_002147"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="OO199_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5536175.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO199_002148"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OO199_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5536176.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO199_002149"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OO199_002149"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5536177.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO199_002150"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OO199_002150"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5536178.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO199_002151"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OO199_002151"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5536179.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO199_002152"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OO199_002152"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5536180.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO199_002153"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OO199_002153"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5536181.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO199_002154"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OO199_002154"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5536182.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENTFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO199_002155"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OO199_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5536183.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="OO199_002156"
     CDS             complement(27384..27845)
                     /locus_tag="OO199_002156"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5536184.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="OO199_002157"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="OO199_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5536185.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="OO199_002158"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="OO199_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7087 a         7856 c         7299 g         6994 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaagcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagta ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAJTNV010000048        29049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNV010000048 DAJTNV010000000
VERSION     DAJTNV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO199_002159"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO199_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5536186.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3188
                     /gene="mdtO"
                     /locus_tag="OO199_002160"
     CDS             1140..3188
                     /gene="mdtO"
                     /locus_tag="OO199_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5536187.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3185..4651
                     /gene="mdtP"
                     /locus_tag="OO199_002161"
     CDS             3185..4651
                     /gene="mdtP"
                     /locus_tag="OO199_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5536188.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4849..5088
                     /locus_tag="OO199_002162"
     CDS             4849..5088
                     /locus_tag="OO199_002162"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536189.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5098..5268
                     /locus_tag="OO199_002163"
     CDS             5098..5268
                     /locus_tag="OO199_002163"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5536190.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5317..6729
                     /gene="fdhF"
                     /locus_tag="OO199_002164"
     CDS             5317..6729
                     /gene="fdhF"
                     /locus_tag="OO199_002164"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5536191.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6730..6996
                     /locus_tag="OO199_002165"
     CDS             6730..6996
                     /locus_tag="OO199_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5536192.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7090..7779
                     /locus_tag="OO199_002166"
     CDS             7090..7779
                     /locus_tag="OO199_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5536193.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO199_002167"
     CDS             complement(7857..9170)
                     /gene="gltP"
                     /locus_tag="OO199_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5536194.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO199_002168"
     CDS             complement(9512..10108)
                     /gene="nrfG"
                     /locus_tag="OO199_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5536195.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO199_002169"
     CDS             complement(10105..10488)
                     /gene="nrfF"
                     /locus_tag="OO199_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5536196.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10481..12199)
                     /locus_tag="OO199_002170"
     CDS             complement(10481..12199)
                     /locus_tag="OO199_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5536197.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO199_002171"
     CDS             complement(12219..13175)
                     /gene="nrfD"
                     /locus_tag="OO199_002171"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5536198.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO199_002172"
     CDS             complement(13172..13843)
                     /gene="nrfC"
                     /locus_tag="OO199_002172"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5536199.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO199_002173"
     CDS             complement(13840..14406)
                     /gene="nrfB"
                     /locus_tag="OO199_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5536200.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO199_002174"
     CDS             complement(14451..15887)
                     /gene="nrfA"
                     /locus_tag="OO199_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5536201.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16280..18238
                     /gene="acs"
                     /locus_tag="OO199_002175"
     CDS             16280..18238
                     /gene="acs"
                     /locus_tag="OO199_002175"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5536202.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18438..18752
                     /locus_tag="OO199_002176"
     CDS             18438..18752
                     /locus_tag="OO199_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5536203.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18749..20398
                     /gene="actP"
                     /locus_tag="OO199_002177"
     CDS             18749..20398
                     /gene="actP"
                     /locus_tag="OO199_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5536204.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20576..21868
                     /locus_tag="OO199_002178"
     CDS             20576..21868
                     /locus_tag="OO199_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5536205.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22022..23671)
                     /locus_tag="OO199_002179"
     CDS             complement(22022..23671)
                     /locus_tag="OO199_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5536206.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO199_002180"
                     /gene_synonym="yjcD"
     CDS             complement(23822..25171)
                     /gene="ghxP"
                     /locus_tag="OO199_002180"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5536207.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO199_002181"
     CDS             complement(25877..26341)
                     /gene="soxR"
                     /locus_tag="OO199_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5536208.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26427..26750
                     /gene="soxS"
                     /locus_tag="OO199_002182"
     CDS             26427..26750
                     /gene="soxS"
                     /locus_tag="OO199_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5536209.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO199_002183"
                     /pseudo
     CDS             complement(26753..28330)
                     /gene="pdeC"
                     /locus_tag="OO199_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28759..29040
                     /locus_tag="OO199_002184"
     CDS             28759..29040
                     /locus_tag="OO199_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5536210.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7778 c         7349 g         6634 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gctcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtatcggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     1381 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     1441 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     1501 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccatttac gggcaaacct
     1561 tccccgccat gctcgactat ccggaagtag tcgtgcgctt aacgctgtgg tgtatcgttg
     1621 ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt cccaatcgtg
     1681 ccattacgca aatgcatcaa gcgcttaatg atcggcttga tgatgccatt agccacctga
     1741 cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg gcgctgcaaa
     1801 aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc gcatggtggc
     1861 aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac gatcccacct
     1921 cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcttca gaaatcaaca
     1981 agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg atcagtgaaa
     2041 gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg ttgttacaac
     2101 tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca tcaatggtcg
     2161 ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg ctcgcctgtt
     2221 tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc tgtatgctga
     2281 cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg gtgctgcgtt
     2341 ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg gtcatgccct
     2401 ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg ttggccgcat
     2461 ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg gtcaccttcg
     2521 cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa attcgcgatc
     2581 gcgccctggg tatcatcatt ggtaccgtgg tgtccgcgat gatttacacc tttgtctggc
     2641 ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt atgttaagta
     2701 aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat ctgcaaattc
     2761 gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc gtggcgctgg
     2821 agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa acggttattc
     2881 gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg caggcgctgg
     2941 ataacgcact acagccggac agagcaggtc agtttgccga cgccctggag aaatacgctg
     3001 ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag acacccacct
     3061 cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag cttttcgccc
     3121 gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca caaggagcca
     3181 cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg gcagcacgac
     3241 gctgatttcc ggctgtgccc tggtacgtaa ggattctgcg cctcatcaac agctcaaacc
     3301 ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc cgcaggcgca
     3361 gtggtggaaa caactcaatg acccgcagtt ggatgccctg atccaacgga cgctaagtgg
     3421 ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcaatcgc aggccgattt
     3481 gttagatgcc ggttcacagt tgcaggtggc agcgttaggg atgcttaacc gccaacgtgt
     3541 ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg ggatggacgg
     3601 gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg atttgtgggg
     3661 tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc tggcagaaac
     3721 cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca gtatgcaggc
     3781 cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt acgcggtgaa
     3841 agcgcaccaa agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc acggcgcgcg
     3901 ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa tcaccgaaac
     3961 gagagaatct ctgcgcgcat tgattggcgc gggagccagc gatatgccgg agatcaaacc
     4021 tgtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg agttgctcgc
     4081 cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag atcaggtgga
     4141 ttccgcgcgg gcgttgttct atccgagctt tgatatcaaa gcgtttttcg gccttgatgc
     4201 catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca tcccgggtct
     4261 gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca cgcgcgccgc
     4321 cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc gtgacgttgc
     4381 cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg ctgaacacgt
     4441 ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg gcttaaccag
     4501 ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt cattactgat
     4561 gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg gcggcgggta
     4621 tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg ctgtcacgcg
     4681 gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga gttttgaata
     4741 aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa agatgcatac
     4801 tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat gaaaaaagtc
     4861 gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt cgataacggc
     4921 aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct gtgtctgaag
     4981 ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg cctgaaaacc
     5041 cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctgaga tgaggcactg
     5101 aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga tgccatccag
     5161 acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca aaaatttgcg
     5221 cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca cggcccatcg
     5281 gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat taacgaaatt
     5341 gataataccg atttagtgtt cgttttcggg tacaacccgg cggattccca cccaatcgtg
     5401 gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg cgatccgcgc
     5461 aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg ctcgaacatc
     5521 gcgctgttga atgcgatggg ccatgtcatt attgaagaaa atctgtacga caaagcgttc
     5581 gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaagg ctacacgccg
     5641 gagtcggttg aagatatcac cggtgtcagc gccagtgaga ttcgtcaggc ggcacggatg
     5701 tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca gttctaccag
     5761 ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgaccagtaa cctcggtaag
     5821 ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc ctgcgatatg
     5881 ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc taaccgcgaa
     5941 aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggcta tcgcatcagc
     6001 gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat gggcgaagat
     6061 ccgctgcaaa ctgatgcgga gctgtcggca gtacgtaaag cctttgaaga tctggaactg
     6121 gtcatcgttc aggacatctt tataaccaaa accgcgtcgg cggcggatgt cattttgccg
     6181 tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg cttccagcgt
     6241 ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat catcagtgaa
     6301 atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat ctgggatgag
     6361 ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg cgaactgggc
     6421 ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc ttatctgttt
     6481 aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga ctgggtagcg
     6541 ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg tgaagttggt
     6601 cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct ggctgatgaa
     6661 cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga agatgaggca
     6721 ttggtttgag tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt cagcgatcgt
     6781 ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg taacgagctg
     6841 gttaccgaaa gcttaagccc gattacgaaa acgccggaat acaaatactg cgccgtgcgc
     6901 gtcgagccga tctccgatca gcgcgccgcc gagcagtacg tgattgacga gtacaacaag
     6961 ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta cagcctcctt
     7021 tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct acccaatcgt
     7081 attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt tgcccacgcg
     7141 aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga ccgacgtgca
     7201 caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc cgaatattgg
     7261 gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgttggc gcagatcaaa
     7321 atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga gaaagcggcg
     7381 caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa tactcaagcg
     7441 ggtaaaccgg attatccaaa ggcgatttcg ctgttagaaa acgcctcgga agatctggag
     7501 aatgactccg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa cggcgtgggc
     7561 attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc aatttcccga
     7621 accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgagga aggttttatc
     7681 gagaagaaca aacaaaaggc gctgcactgg ttgaacctga gctgtatgga agggtttgat
     7741 accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga taagacgcgg
     7801 caagcgtcgc atcaggcatc cacacattgc cgggggatat ccccggcaat cttcaattat
     7861 tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag cgcctttttg
     7921 cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt acccaccacg
     7981 ttcagcgcag tacgcgccat gtcgaggata cggtcaacac cagcaataaa cgccagacct
     8041 tccagcggaa tacctacgct acccagcgtt gccagcaaca ccacaaacga cacgccagcc
     8101 acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat ttcctgccag
     8161 atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact ttgatacagc
     8221 gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaagctggt gatcgacgcc
     8281 ggtgctccgt aggcttccat cttctcaata attcgcggca gtacgctttc agagctggca
     8341 gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca gacgcttaac
     8401 ccgcacagac gtgccacaat tcccagcact accagcgcga agaacagaat ggcgaaatgc
     8461 accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt agccaccgtc
     8521 accgcaatca gcgcaaacac acccaccggt gcataacgca tcaccatgtg agtcacttta
     8581 aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg cgtcgcgggc
     8641 agggaagaaa gccccagacc aaacagcacc gagaaaaaga tgatcggcag catttcgcct
     8701 ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc cataatgccg
     8761 tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc gacggtcgcc
     8821 agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt gatccccaaa
     8881 atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc aatacgcccg
     8941 agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat cacaatcggc
     9001 acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag caaattgacg
     9061 accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag aatgcccagc
     9121 accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat cagaataact
     9181 tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac tgccagggca
     9241 tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta tcatttgcgc
     9301 ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca taataacgcg
     9361 agtttatggc aggattaatg aataaccatt tattatggaa agatttttat ttgtttaatc
     9421 tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat tttttatgca
     9481 gctcaatgac ataatcttca tcaccagatg attaatccga tctccgctgc aacagttttg
     9541 ccatattaat cgactcgacc agctgtgtcc ggttgatccg gggtgagttg agatccatca
     9601 ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca tcggaagcca
     9661 gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg atcattgcgc
     9721 gagtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc agcgccgcat
     9781 acagttcagc gttctcacca cgtagttgca acgcctgacg atacgccagc agcgaattgc
     9841 tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccattgttcg ctgttttgtg
     9901 gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggggtt tgctggctgg
     9961 caaactgatg tagcggatcg cgctgacgct gatactccgc acgtaccgcc tgccattttg
    10021 ggcttaacag ataactgccg acacagagga ataccatcaa tatcgtgagt gttgtcagca
    10081 ttttcaccgg tatttgaggc tgtttcatcg cttagccctc actcgccaga ggatcagtgc
    10141 catcagcaat aacaacacca ctggcagcgc ccacaacacc agcgtctgac ccgttaacgg
    10201 tgggttatag cgaacaaaat ctccgtagcg ttcggtcatc cagccaatga tttcgacttc
    10261 gttcttcccc tccgacacca tgctataaac ctgatggcgc atactgaccg ccaccggcgc
    10321 attggattcg agtaagtttt gattctggca ctgcggacaa cgtaactggc tggcaatatt
    10381 taacgcctgt tgctgttgtt gcggattggc gaattgccag gtgtctacaa cctgagcgcg
    10441 cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg cgcttcttcc
    10501 cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa tcctcccccc cagatccagc
    10561 gcacaccgct ttgtacatac aaacgaaaag cgtaacgatc cgctctggtt ttttcaccca
    10621 tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc atttgctgac
    10681 ggcgtgcttc ataaaaacgc cgctccggcg ttaattcacc aatgcgttgc tgatggtcaa
    10741 acagcgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga tcgagtcgct
    10801 caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa ttcaggctga
    10861 tctcctgacg actgacactg gaaaccacga tccccgcggc aaataacagc acgccagcat
    10921 gagctaccag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc agcacaatca
    10981 ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc gcgccaacgg
    11041 agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc accagcacga
    11101 tcagtagcac tgcgcaaaac agcaacagcg tagcgaggat taacatttcc cgcgataacc
    11161 ccgaaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga gccaaagacg
    11221 caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta tccagtgcga
    11281 acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtgcccagc agcgacagca
    11341 tcagagtaac tatcgccagc aacagcgacc agtggcggaa aatcccccgc tggcgtgtca
    11401 gggacaaact gtgcagcagc gcagtggcag aaagccaggg taataaagag gcgttttcca
    11461 ccggatccca gaaccaccag ccgccccagc cgagttcgca gtaggcccac caggaaccga
    11521 ggatgatccc cgccgttaat gcactccagc caggtaacgc ccagcgccag caaattcggg
    11581 cgctggccgc atcaaactcg ccgcgcagta gactcgccag cgccacgctc gccgctacca
    11641 tcaaaccgcc atagccaagg caaagcagcg gtggatgaaa gataagaccg ggatgttgca
    11701 gcatcggatt gagatcgcgg ccttcgattg ctggtggaaa tatccgcacg aagggatcgg
    11761 accacagcac cacaaacagt agcagtgcgg cgagcatgag agataaaacg gctagcgtca
    11821 gcggaaatag cggatcgctt tgctgccgat aatgccaggc aaacagcgcg ctccaggcgg
    11881 aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc gccgccagtt
    11941 tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc gagaaatcgc
    12001 tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg aactgcgcca
    12061 gaatgccgat gcaagtgagt cgcatcatgg caggccagcg caaccgcact cccgcaaagg
    12121 ccgtcaacgg ggtcaacacg ttgaccccga gacttaacaa caacgccaga aaaccgactt
    12181 caggaaggaa tacgtccaga aaccacctct tttctggctt acgccaccgt caactgtccg
    12241 gcgtagagaa tgaaaaagcg cagcatcaac acgccgacca gactggcccc acacgccgcc
    12301 agcacggcag gaatgccgga actgcgattg acccacggtt tgagcaacat tggcacaatc
    12361 agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc acctaatgcc
    12421 gccaccagcg cacgaacttt accgtcatcg cccagcgcca gaccgacaaa aaacgccacc
    12481 agcaggaaga tttcacccca taccacgggg atttccatcc ggtgaacaaa gtgcgcttcc
    12541 gtggaatgcg ggttactgcg ttggcgtatc gccatggcga tcagcgccac cgccgcaccg
    12601 gacgagatgc cggagaagag gagcagcacc ggcaggctcg ggttattgag gaacggatac
    12661 gatttcagcg ccgacagcag aaagccggta taagccccca gcagcaccgc caacaccagc
    12721 atcagcgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt ttgcacgatc
    12781 cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa gattttcgcc
    12841 agccacagca ccagcaccac catgtagagc tgaaacagca tcacccccat cgacatcacc
    12901 gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt caggtggaag
    12961 acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt ggtgcgcagc
    13021 aacgtactgt ctgcaccgcc cgcctgcggg tagaagcgac gtaacagcac ggccagcgtc
    13081 accagaccgg cagaaatacc aatcaaaaac aggtagatgg caatcggcca gtcccacacc
    13141 agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg tatttaaacg
    13201 gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa gtaggcttct
    13261 ggcgcagcag ttgcgaaatc tcactgttag gatcgtccag attgccaaac gtcagcgcct
    13321 tcgtcgggca agcttcaacg cacgcgggaa gcttaccggc ctgcaaattg gtcttacgac
    13381 agaaatcgca tttgtccgcc gtcttcgtga ccggatggat aaagcgcacg cggtacggac
    13441 aggcggcgat gcagtactga caaccgacgc agagatcagg attaacatcg acaatcccgc
    13501 tagcggcatc acgaaaagac gcaccggtcg ggcagacgtc aacgcacggc acatgatcgc
    13561 agtgctggca agacttacgg aagaagcgat atttcacatc aggaaattcg ccctgcggct
    13621 cgctacgaat aatcgtcagg cgcgagacgc cttccggcac cttgttcact tcccgacaag
    13681 catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg ccgtaacgca
    13741 ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg acggctgcca
    13801 gcacgccgac gccggtgaga aactggcgac gagaccaggt catggctgct ccttaagcaa
    13861 cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc aatcgacgca
    13921 aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg gatgcaggga
    13981 atggcagctg gcgcacgcca ctttggttac gtggacatcg tgcggccaga acgctttttg
    14041 caactgttcc ggcagatgac aggacataca gacgctgttc tgctctccga ccttatacat
    14101 cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg atgcttggcc
    14161 gtgacagttg gtgcaggtga ccggcaattt gttattcggg ttgataacgg aagcatgttt
    14221 gccatgcata ccttcggtat ctggtttatg acagtccaga caggcggcat ccgggttacg
    14281 ctgctgggta acttcgtagc gatcaccaga tgcctgcacc gcaggggtag cggtaattcc
    14341 gttcaggctc cacaacaggc ccgacgccag caccccagca gttaataacg aacgtaatac
    14401 gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg ttattggctt
    14461 aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt gatgaagtcc
    14521 tgcttctcgg ccttgatttg ttccatgttc aggccaatgg cctgctgggc tttctctttg
    14581 gtcgagatat ccgggatctg gatttcatgg gtgatacctt tggtcgccag caggcgcgcc
    14641 agtttggtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatccg tagaccttct
    14701 tccggtgcgt gcatatgaat accgtgggaa gcgatcgcca gatcccaacg ccactgggca
    14761 tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc atccagcgcc
    14821 gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt caggtcgtta
    14881 atcgattgct tacgttccgc gaccactttt tgcaaggcag ctttatcctg ggtatggcag
    14941 ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc ggtgtagagt
    15001 ttgccttcgg cgttctgcac ttttggcata tggcagtcga tacaggtcac gttgttttta
    15061 ccgtgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag cattggcgtt
    15121 ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg ctccatattt
    15181 tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc gtcgaagtaa
    15241 tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc ggctttctca
    15301 aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag ggttaactcc
    15361 ggtttgcctt tggcgaactc tggtgaggcg gtgttatggc agtcggcaca acctaagttg
    15421 ttgacgattt ccgggccgcc gcgcgcccat ttaccgtgga agtagccatc ttcgccgtct
    15481 ttctggatca gacgcgccac atccgggctt ttacaactcc agcacgccat cggtagcgga
    15541 ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc ggtcacagca
    15601 aaggcatgtc cacgaggctt gttgtaatcg cgcgagaagg gataccccgc ccacaggatc
    15661 accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga ggtggctttc
    15721 caggagagat attgatcgtg atgctgcggg gcaaaggttt cattcttcgc ttccacagtt
    15781 acaggttttg cgggagctgc cgtttgttca gcgtgaacag aagtgaaaaa gaaaaaagga
    15841 atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg ggcttcatcc
    15901 gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta accgcaccta
    15961 cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg aatgttgtct
    16021 ttaatcaatt gtaagtgcat gtgaaatacc actttagagt tagtcagtat cttcctcttt
    16081 ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg ttggtgacct
    16141 tcaaaaatta ccctgccgtt tatttgcacc attctacttt tgcgtgatct gtcgcccaaa
    16201 tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat tattaacatc
    16261 ctacaaggag aacaaaagca tgagccaaat tcacaaacac accattcctg ccaacatcgc
    16321 agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat ctattaacgt
    16381 acctgatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac cgtaccagaa
    16441 ggtgaaaaat acctcctttg cccccggtaa tgtgtccatt aaatggtacg aggacggcac
    16501 gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg atcgcaccgc
    16561 catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata aagagctgca
    16621 ccgcgacgtc tgccgcttcg ccaataccct gctcgagctg ggcattaaaa aaggtgatgt
    16681 ggtggcgatt tatatgccga tggtgccgga agccgcggtt gcgatgctgg cctgcgcccg
    16741 cattggcgcg atgcattcgg tgattttcgg cggcttctcg ccggaagccg ttgccgggcg
    16801 cattattgat tccaactcac gactggtgat cacttccgac gaaggcgtgc gcgccgggcg
    16861 tagtattccg ctgaagaaaa acgttgatga cgcactaaaa aacccgaacg tcaccagcgt
    16921 agagcatgtg gtggtactga agcgtactgg cggaaacatc gactggcagg aagggcgcga
    16981 cctgtggtgg cacgacctgg ttgagcaagc cagcgatcag caccaggcgg aagagatgaa
    17041 cgccgaagat ccgctgttta ttctctatac ctccggttct accggaaaac caaaaggcgt
    17101 actgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat atgtctttga
    17161 ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta ccggacacag
    17221 ttatttgctg tacggcccgc tggcctgcgg cgcgaccacg ctgatgtttg aaggcgtacc
    17281 gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg tcaatattct
    17341 ctataccgca cccacggcga tccgcgcgct gatggcggaa ggcgataaag cgattgaagg
    17401 caccgaccgt tcgtcgctac gcattctcgg ttccgtgggc gagccaatta acccggaagc
    17461 gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgt ccggtggtcg atacctggtg
    17521 gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg agctgaaagc
    17581 cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata acgaaggtaa
    17641 cccgctggaa ggggctaccg aaggtagcct ggtgatcacc gactcctggc ctggtcaggc
    17701 gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tatttttcca ccttcaaaaa
    17761 tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact ggatcaccgg
    17821 gcgtgtggac gacgtgctga acgtctccgg tcaccgtctg gggacggcag agatagagtc
    17881 ggcgctggtg gcgcatccga agattgccga agccgccgta gtaggtattc cgcacaatat
    17941 taaaggtcag gcgatctacg cctatgtcac gcttaatcac ggggaggaac cgtcaccaga
    18001 actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg cgacgccaga
    18061 cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta tgcgccgtat
    18121 tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga cgcttgccga
    18181 tcctggcgtg gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc catcgtaacc
    18241 cacaattgcc ggatgcgaat cggtaacggt ttgtaggcct gataagacgc gacagcgtcg
    18301 catcaggcat tgattgccgg atgcggcgta aacgccttat ccggcctaca ttcggcaagg
    18361 gttacccgag cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta
    18421 cctctggaga atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt
    18481 tcagggagtt agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg
    18541 cagtttatat cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcacgccgc
    18601 tgaatccgaa caccagcgtc acacgcggta ttccaattgg tgttggagtg attgtgatct
    18661 cctttgttct caccggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata
    18721 atgaagtcct gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac
    18781 actccctttc gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac
    18841 gaactggcag gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta
    18901 ctgggcatca aaacgcgtac gttctcgtag cgactactac accgcaggcg gcaatatcac
    18961 tggcttccag aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg
    19021 gatctccgcg ctggtgttta cctccggcta tgacgggctg atctactcgt tgggcttcct
    19081 ggtgggctgg ccaatcattc tgtttctgat tgccgaacgt ctgcgtaacc tgggacgcta
    19141 cacctctgcc gatgtggcct cttatcgtct gaaacaaggg ccgattcgta ttctttcggc
    19201 ctgtggttct ctggtagtgg tggcgcttta ccttatcgct cagatggtag gcgcaggtaa
    19261 actgatcgag ctgctgtttg gccttaacta tcacattgcg gtggtgctgg tcggcgtgct
    19321 gatgatgatg tacgtcctgt tcggcggcat gctggcgacc acctgggtac aaattatcaa
    19381 agctgtgctg ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg
    19441 ctttagcttc aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat
    19501 catgaaaccg ggcggactgg tgaaagatcc gatctccgcg ctctctctgg ggctgggact
    19561 gatgtttggt acggcgggct tgccgcacat tctgatgcgc ttctttacag tcagcgatgc
    19621 ccgcgaagca cgtaagagcg tgttctacgc caccggattt atgggctact tctatattct
    19681 gacctttatt atcggcttcg gcgcgatcat gcttgttggt gcgaatccgg aatataaaga
    19741 cgcggcgggc catctgattg gtggtaacaa catggcggcc gttcacctgg cgaatgcagt
    19801 gggcggcaac ctattcctcg gttttatttc agcggttgct ttcgccacta tcctcgcggt
    19861 ggttgcggat ctgacactgg cgggcgcatc ggcggtttcg catgacttgt acgctaacgt
    19921 cttcaaaaaa ggcgcgaccg aacgtgaaga gctgcgggta tcaaaaatca ccgtactgat
    19981 cctcggcgtg attgcgatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat
    20041 ggtaggcctt gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat
    20101 gtactggtcg aaactgacta cccgtggcgc gatgctgggc ggctggctgg ggctgattac
    20161 cgcagtggtg ctgatgatcc tcggcccgac tatttgggta cagatcctcg gtcacgaaaa
    20221 agccatcttc ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggcat
    20281 ctggttcttc tcggcaaccg ataactcagc ggaaggggcg cgcgagcgtg aactgttccg
    20341 cgcgcagttt atccgctccc agaccggctt tggcgttgag caaggccgcg cacattaatc
    20401 tctccccttc cccggtcgcc tgaccgggga atacgcttcc tctccaacat gcatcacctt
    20461 ttcccataat attaaacaaa taaactcatt aaaaaatgag cgatttttga cagtcgtaga
    20521 aaatgataat gcagagaata tgccttttct ttcttgttaa ttataaggat attttatgcg
    20581 ctataatggt ttaaataata tgtttttccc tctttgccag attaacgata accactctat
    20641 cacaagttca tcacatacaa agaaaacaaa atcagataat tacagcaaac atcataaaaa
    20701 cacgttaatt gacaataaag ccctctctct tttcaaaata gatgatcatg aaaaagtgat
    20761 aggcttgatt cagaaaatga aaagaattta tgatagttta ccatcaggaa aaatcacgaa
    20821 agaaacggac aggaaaatac ataaatattt tatagatata gcgttatatg caaataataa
    20881 atgtgacgat agaattacga gaagagttta ccttagtaaa gaaaaggatg tatccattaa
    20941 ggtggtatat tttataaata atgtcgccgt ccataataat actatcgaaa ttccacagat
    21001 agtaaatggt ggttacgatt tttcacacct tagcctgaag ggtatcgtga ttaaagatga
    21061 agatttatcc aattcgaatt ttgcaggttg cagactacaa aacgctattt tccaggactg
    21121 taatatgtat aaaacgaatt tttattacgc cataatggaa aaaatacttt ttgataattg
    21181 tattctcgat gactcaaatt tcgctcagat aaaaatggcc gacggaactc taaatgcatg
    21241 ctccgctatg catgttcaat tctacaatgc agcaatgaat agagccaata ttaaaaatac
    21301 cttccttgac tattcaaatt tttatatggc gtacatggct gaggtaaatc tttataaagt
    21361 aatagcgcca tatgttaatt tatttaaagc cgaccttagt ttctctaaac tcgatttaat
    21421 taactttgaa catgctgatc tgtctcgcgt caatctgaac aaagcaatcc tccagaatat
    21481 aaacttaatt gatagcaaac tcttttgtac gtggctgaca aatacattcc tcgaaatggt
    21541 tatatgtacc ggctctaata tggctaatgt taattttaat aatgccaatt taagcaactg
    21601 ccatttcaac tgttctattt taacaaaagc ctgtatgttt aatacccgtc tctatcgggt
    21661 taatttcgat gaggctagcg tccagggaat gggcatttcc attctccgtg gggaggaaaa
    21721 tatccccatt gatagtgata ccctggtaac actacagaaa ttctttgaag aagattgtac
    21781 ctctcatact ggcatgtcac aaactgagga taatattaat gcagtcgcta tgaagattac
    21841 tgcagatatt atgcaacacg cagattgagg ttaagtcgaa gaacagccaa tgaattgact
    21901 ttacaagaga gataacttat ttgttttaga atcatgaatg ttcgcccgag ctaacctgtt
    21961 aaaggtgcaa aagattgcac ctttttcaga ttacctcggg cctggttacg tcgctttcgg
    22021 cttactgatt ttcctcaatt agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    22081 gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    22141 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    22201 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctgacctccg
    22261 taagcaactg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    22321 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttct tctttcaatt
    22381 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    22441 caaacagcga gaagagaatg acgccagccg ccaggaacac cagctcatag cgcgccggga
    22501 agacgttacc atccggcaag agcagcggga ttgagagcac accggccaga gtgatcgccc
    22561 cacgcacccc agcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    22621 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    22681 ggaccagcat cagcgccgca tatatcagaa taatatcggt aaacagcatc cagatttcga
    22741 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agttgcagac
    22801 ctaacagcag gaacaccatg ccgttaaaga caaattccag catcgcccag gtgctgtttg
    22861 cgcgcaggcg cattgccagt ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc
    22921 cggcggcaac cgcagcgagg atgcccgaaa cgccaatatg ttcggcaatc agataagaag
    22981 cgaatggcag caagaacagc agaacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    23041 tgaggaatcg cagcgagcga ccatacagcc agctcaccac aaaaccggca agaataccgc
    23101 caatggctac tttcataaat tcgaccgtcg caccgccgac agtgaagatc atcgtcccca
    23161 tcgccactgc cacggcaaac ttcaacgaca ccaggccgga agcgtcattc atcaacgctt
    23221 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atcccggaga
    23281 gcgccacagc atcggtcgga gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa
    23341 tgcccggcac cacccagtaa ataaggaagc caatgccgac cacggtgacc accaccagcg
    23401 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    23461 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    23521 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    23581 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    23641 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    23701 tctgagaaag acgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    23761 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat ccgatgaaag
    23821 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacggcgata acgaccgtac
    23881 cgatgttcaa cttgcgccat tcaccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    23941 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    24001 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttgctc agcatcagca
    24061 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gaaagcggag
    24121 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaac ccggttttac
    24181 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    24241 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    24301 gcccatcttt atccagcagg ttcgcctggc cggcgacagc gcggatagtt ccggtggcat
    24361 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    24421 ccataatgtc caggctgcca atcagtgaat tgccgttttc atcgctcaat gaaggcatgg
    24481 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    24541 taatggtcag cagaatgcca ccagggactt tcagtttttc caagccgatg atcaccgcca
    24601 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
    24661 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
    24721 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    24781 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
    24841 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
    24901 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    24961 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
    25021 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
    25081 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    25141 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    25201 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    25261 tgcggcaatt tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    25321 ggtaaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
    25381 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
    25441 tcgtcgatgt gcaagattgc agcgttatcg agccgtatac cgttctggat tgcgataaaa
    25501 aatcgtctga gatgacaaaa atattgcggc taaaaagtat acttttacac tggcgaagaa
    25561 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
    25621 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgttcca gcgggataga gagaaagaca
    25681 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgtgtt tgcggataat
    25741 tccaaaagag ctcgatcagt cccctcgccc cttcggggag agggttaggg ttaggggaac
    25801 aggcagcacc agtgcaaata tttaccctca ccccggcccc atagttaacg atgagtcagc
    25861 ttgtccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
    25921 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
    25981 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
    26041 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttctg
    26101 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcgattt
    26161 tgataattgc gacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
    26221 ggatactagt aatcaaccct ttgctttcat agaaatgcag cgccgatacc gccacaccgc
    26281 tgcgtttcgc cacttcgcca ggggttagca gcgctttaat gcggggtaat ttcttttcca
    26341 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
    26401 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
    26461 catggattga cgtgcatatt gaccagccgc ttaacattga tgtcgtcgca aaaaaatcag
    26521 gctattcaaa gtggtacttg caacggatgt tccgcacggt gacgcatcag acgcttggcg
    26581 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
    26641 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
    26701 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtag ttttattgcc
    26761 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca
    26821 tcgccttcgc aaaaaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
    26881 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
    26941 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
    27001 atttatcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
    27061 caataccaaa atcatcaatt gccacttcgt aaccagcctg gcggaaagcc agaattaatc
    27121 ggcgtcattt tttcgacatc aagaaatgca tgctcagtca cttcaaattt aatttgctgc
    27181 ggacgcaccg cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga
    27241 aaatcggagg ccgacaggtt aatagaaaca tagcgatcgg catgtgttgc caggtacgcg
    27301 cccagatcgc ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc
    27361 ttttctgcca gcggaataaa ctctgccgga ttcattattt gcccctgctc accaggccaa
    27421 cgtaacaacg cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa
    27481 taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag
    27541 ttttgtcgaa tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc
    27601 aacgcaaaaa taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca
    27661 atggctgcaa ttgggcgttt tgtcgaataa attgtcgcat ataaatagcc atttctttgt
    27721 acggttaaat ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt
    27781 aacgagaaaa aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca
    27841 gacatcactt cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata
    27901 taggtcattt tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta
    27961 ggttcacgct tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat
    28021 gagcataaaa aatggttatc acgaattcat taatataaag atagccacga ataatattca
    28081 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
    28141 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
    28201 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
    28261 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
    28321 gatgactcat tgctatcaat ttcctgcctg tttataccca gccattgcaa ggtgaaaccc
    28381 tggctaccga cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa
    28441 attgatacct ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt
    28501 ttctacagga atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat
    28561 aatttgtaca gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat
    28621 agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg
    28681 atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg catcggctct
    28741 tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct tcgctggcaa
    28801 cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt tcgtcggtct
    28861 gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc ctgttggttc
    28921 gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca caacatcgag
    28981 tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat tttgacctga
    29041 tacgtagaa
//
LOCUS       DAJTNV010000049        28431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNV010000049 DAJTNV010000000
VERSION     DAJTNV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            564..1334
                     /locus_tag="OO199_002185"
     CDS             564..1334
                     /locus_tag="OO199_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5536211.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO199_002186"
     CDS             1325..1798
                     /locus_tag="OO199_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5536212.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO199_002187"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO199_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5536213.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO199_002188"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO199_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5536214.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO199_002189"
     CDS             3233..3727
                     /locus_tag="OO199_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5536215.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO199_002190"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO199_002190"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5536216.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO199_002191"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO199_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="OO199_002192"
     CDS             7571..8485
                     /locus_tag="OO199_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5536217.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="OO199_002193"
     CDS             8700..10061
                     /locus_tag="OO199_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536218.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO199_002194"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OO199_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5536219.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO199_002195"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OO199_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5536220.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="OO199_002196"
     CDS             12611..13900
                     /locus_tag="OO199_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5536221.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="OO199_002197"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="OO199_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5536222.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="OO199_002198"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="OO199_002198"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5536223.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="OO199_002199"
     CDS             16226..16606
                     /locus_tag="OO199_002199"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5536224.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="OO199_002200"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="OO199_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5536225.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANDVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="OO199_002201"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="OO199_002201"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5536226.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="OO199_002202"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="OO199_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5536227.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="OO199_002203"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="OO199_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5536228.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="OO199_002204"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="OO199_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="OO199_002205"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="OO199_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="OO199_002206"
     CDS             23335..23847
                     /locus_tag="OO199_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5536229.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="OO199_002207"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="OO199_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5536230.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="OO199_002208"
     CDS             26440..26643
                     /locus_tag="OO199_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5536231.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="OO199_002209"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="OO199_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5536232.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28431)
                     /locus_tag="OO199_002210"
     CDS             complement(27679..>28431)
                     /locus_tag="OO199_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5536233.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6961 a         7432 c         7125 g         6913 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acgacgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccaag atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag t
//
LOCUS       DAJTNV010000050        28406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNV010000050 DAJTNV010000000
VERSION     DAJTNV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO199_002211"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO199_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5536234.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO199_002212"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO199_002212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5536235.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO199_002213"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO199_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5536236.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO199_002214"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO199_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5536237.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO199_002215"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO199_002215"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5536238.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="OO199_002216"
     CDS             6428..7057
                     /locus_tag="OO199_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5536239.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO199_002217"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO199_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5536240.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8732..10075)
                     /gene="melA"
                     /locus_tag="OO199_002218"
     CDS             complement(8732..10075)
                     /gene="melA"
                     /locus_tag="OO199_002218"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5536241.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="OO199_002219"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="OO199_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5536242.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="OO199_002220"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="OO199_002220"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5536243.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="OO199_002221"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="OO199_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5536244.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="OO199_002222"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="OO199_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5536245.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="OO199_002223"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="OO199_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5536246.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="OO199_002224"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="OO199_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5536247.1"
                     /translation="MKILIVEDDMLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="OO199_002225"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="OO199_002225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5536248.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO199_002226"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO199_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5536249.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO199_002227"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO199_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5536250.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="OO199_002228"
     CDS             complement(21748..22626)
                     /locus_tag="OO199_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5536251.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO199_002229"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO199_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5536252.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="OO199_002230"
     CDS             25253..25588
                     /locus_tag="OO199_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5536253.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="OO199_002231"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="OO199_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5536254.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="OO199_002232"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="OO199_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5536255.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="OO199_002233"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="OO199_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5536256.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28406
                     /locus_tag="OO199_002234"
     CDS             28257..>28406
                     /locus_tag="OO199_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5536257.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7296 a         7422 c         6935 g         6753 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagcactga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctgacagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca actagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccgcgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gtatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg caactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atatgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcattagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttacg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca accttc
//
LOCUS       DAJTNV010000051        28270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNV010000051 DAJTNV010000000
VERSION     DAJTNV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..605)
                     /gene="capU"
                     /locus_tag="OO199_002235"
     CDS             complement(<1..605)
                     /gene="capU"
                     /locus_tag="OO199_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5536258.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
     gene            complement(608..1450)
                     /locus_tag="OO199_002236"
     CDS             complement(608..1450)
                     /locus_tag="OO199_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5536259.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1636..2353
                     /locus_tag="OO199_002237"
                     /pseudo
     CDS             1636..2353
                     /locus_tag="OO199_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3026..4534
                     /gene="ltrA"
                     /locus_tag="OO199_002238"
     CDS             3026..4534
                     /gene="ltrA"
                     /locus_tag="OO199_002238"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5536260.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4615..5102
                     /locus_tag="OO199_002239"
                     /pseudo
     CDS             4615..5102
                     /locus_tag="OO199_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5185..5442
                     /locus_tag="OO199_002240"
     CDS             5185..5442
                     /locus_tag="OO199_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536261.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(6000..6767)
                     /gene="fecE"
                     /locus_tag="OO199_002241"
     CDS             complement(6000..6767)
                     /gene="fecE"
                     /locus_tag="OO199_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5536262.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6768..7724)
                     /gene="fecD"
                     /locus_tag="OO199_002242"
     CDS             complement(6768..7724)
                     /gene="fecD"
                     /locus_tag="OO199_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5536263.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7721..8719)
                     /gene="fecC"
                     /locus_tag="OO199_002243"
     CDS             complement(7721..8719)
                     /gene="fecC"
                     /locus_tag="OO199_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5536264.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8716..9618)
                     /gene="fecB"
                     /locus_tag="OO199_002244"
     CDS             complement(8716..9618)
                     /gene="fecB"
                     /locus_tag="OO199_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5536265.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9663..11987)
                     /gene="fecA"
                     /locus_tag="OO199_002245"
     CDS             complement(9663..11987)
                     /gene="fecA"
                     /locus_tag="OO199_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5536266.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12074..13027)
                     /gene="fecR"
                     /locus_tag="OO199_002246"
     CDS             complement(12074..13027)
                     /gene="fecR"
                     /locus_tag="OO199_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5536267.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13024..13545)
                     /gene="fecI"
                     /locus_tag="OO199_002247"
     CDS             complement(13024..13545)
                     /gene="fecI"
                     /locus_tag="OO199_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5536268.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13843..14025
                     /locus_tag="OO199_002248"
                     /pseudo
     CDS             13843..14025
                     /locus_tag="OO199_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14019..14123
                     /locus_tag="OO199_002249"
                     /pseudo
     CDS             14019..14123
                     /locus_tag="OO199_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14463..14720
                     /locus_tag="OO199_002250"
     CDS             14463..14720
                     /locus_tag="OO199_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5536269.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15040..15171)
                     /locus_tag="OO199_002251"
                     /pseudo
     CDS             complement(15040..15171)
                     /locus_tag="OO199_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15453..16811
                     /locus_tag="OO199_002252"
     CDS             15453..16811
                     /locus_tag="OO199_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5536270.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17050..18435)
                     /locus_tag="OO199_002253"
     CDS             complement(17050..18435)
                     /locus_tag="OO199_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5536271.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18485..18832)
                     /gene="tnpB"
                     /locus_tag="OO199_002254"
     CDS             complement(18485..18832)
                     /gene="tnpB"
                     /locus_tag="OO199_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5536272.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18829..19209)
                     /locus_tag="OO199_002255"
     CDS             complement(18829..19209)
                     /locus_tag="OO199_002255"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5536273.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19564..19998)
                     /locus_tag="OO199_002256"
     CDS             complement(19564..19998)
                     /locus_tag="OO199_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5536274.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19986..20387)
                     /locus_tag="OO199_002257"
     CDS             complement(19986..20387)
                     /locus_tag="OO199_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536275.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20647..21216)
                     /locus_tag="OO199_002258"
     CDS             complement(20647..21216)
                     /locus_tag="OO199_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5536276.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21961..22104)
                     /locus_tag="OO199_002259"
                     /pseudo
     CDS             complement(21961..22104)
                     /locus_tag="OO199_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(22438..22704)
                     /locus_tag="OO199_002260"
     CDS             complement(22438..22704)
                     /locus_tag="OO199_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536277.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            22845..23051
                     /locus_tag="OO199_002261"
     CDS             22845..23051
                     /locus_tag="OO199_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5536278.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            23146..23748
                     /locus_tag="OO199_002262"
     CDS             23146..23748
                     /locus_tag="OO199_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536279.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            24948..25865
                     /locus_tag="OO199_002263"
     CDS             24948..25865
                     /locus_tag="OO199_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536280.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            25950..26822
                     /locus_tag="OO199_002264"
     CDS             25950..26822
                     /locus_tag="OO199_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5536281.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            27040..>28270
                     /locus_tag="OO199_002265"
     CDS             27040..>28270
                     /locus_tag="OO199_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5536282.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVILRCT"
BASE COUNT         6902 a         7342 c         7383 g         6643 t
ORIGIN      
        1 aattcgtgcc ctttttccgg gcgcaacatt cctacctgag caattacggg gcatcctctt
       61 cgggacgcca gccaggaaag aacactcgga ggcaaactgt ttcgggaatc aacgtataat
      121 tcctggaaat caaagccagg aggcacaaca gtaacccgag ttcgacatcc ttcctgctcc
      181 agatgtgtct tcatatttgt tccgggaaca atcacctcat cgcaaaaatg atttattgaa
      241 aaaaattttg tttttcgtgt caaatatgtc ttttgcctga taattctgaa tggatgtttc
      301 caggtaaaaa accgtaccaa accaacaata ttactatcat gtccactgtg acaaacgatt
      361 gcatcgggct gaaaactatg aactattccg agtaatctcc atacagtagg aatgtgaaga
      421 ctgtttcgaa ataacgcgaa tgtgatatca attccaaact tactggcttc aaaagcaatt
      481 ttgctgttct ccctgcagac aagcagaact gaatgcccca ttttcttcag ggccttcatt
      541 tgagcaacag cctgtaattc ctgaccacct atattcggtg atgattccgt aaatagtata
      601 ttcattatta gtcatccggt aatcttggcc ccttatgaag ggccagcaac gatgaaaaaa
      661 acggtgttgt ataataaaaa agtcgtcttt tcagccatac gcaattttcc cgttccttgg
      721 tgcttattct tcctatccgt gtagttcctt tagcaggagc attcattctt ctttctgttg
      781 tatataaata atggaatcca agttcttcag cgatctgaat ataatccttg ttataataac
      841 cttccggcca acacaaatgt ttactgcatt ttcctgtcat tttttttaaa tattctctcc
      901 cgctcaataa gtcctgccga agatgtttac actgttcttc tcgtgaagtg aactttttat
      961 cccaacgtgt atgagtatgt gtatgaacat gaaattcgac cagaccagat tgcaacattt
     1021 cgttcacttc agaccagcga agcataacat tatcagcatt cccggttgct atttgatgtt
     1081 cgcaatcgcg atgagaatat tcctttccag gagagtgacg aaccggacca tttccaataa
     1141 aactggtaat taaaaaaata tgtgccttga gattaaactc gtttaataaa ggatatacct
     1201 gaaaccagtt gtcaaggtat ccatcatcaa atgtcaacat aacgctttta cggggaagtt
     1261 ttccgccccg ataaaaaaac tccagttcat ctgatgacag tgttttccag ttgttttcag
     1321 ccaaccattt catttgtttc cggaatgtct cgggagaaag tgtaaccagt cctgggcaat
     1381 ggctgacatg atgatacatt aataccggaa catgatttgc tttaaacaaa atgccccctc
     1441 cgtttaaaat ttaaagtttc tggcacccag taagtagaga taaaaggaaa gcctaataaa
     1501 ggtggtgaac tttatatact actaaatcaa acatcaacaa tttataaaca ataatatatt
     1561 atcatatctt ttaaaaacgg ctcactaaga accttgaggt ggcacactaa atttagctac
     1621 ctgaacagag gtgatatgct cacctcagaa caacacaggt gccataatga aaaaagaaat
     1681 ttcagcgcag agtttaaacg cgaatccgct caactggtcg ttgaccagaa atacaccgtg
     1741 gcagatgcag ccagcgctat ggatgtcggc ctttccacaa tgacgcgatg ggtgaaacaa
     1801 ttacgtgatg agcggcaggg aaaaacacca aaagcctccc ccattacccc ggaacaaatt
     1861 gaaatccgtg agctcaggaa aaagctacaa cgtattgaaa tggaaaatga aatattaaaa
     1921 aggctaccgc gctcttgatg tcagactccc tgaacagttt tcgataatcg ggaaactcag
     1981 ggcgcgttat cctgtggcca ctctctgcca tgtgttcggg gtccatcgca gcagctacaa
     2041 atactggaaa aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagccaggt
     2101 acttgaactg catggcatca gccacggctc tgccggagca agaagcatcg ccacaatggc
     2161 aacccagaga ggctaccaga tggggcgctg gcttgctggc agactcatga aagagctggg
     2221 gctggtcagt tgccagcagc cgactcaccg gtataagcgt ggcggtcatg agcacgttgc
     2281 tatcccgaat catcttgagc gacagttcgc cgtaacggaa ccaaatcagg tgtggtgcgg
     2341 tgatgtgacc tatagtgtgc ccggagttca gggcgggcat ggatgcttaa atgaaccgcg
     2401 agtctgtctg gaatattgaa ccggtaactc acgatgagaa acccaacaat cccaccgggt
     2461 gtgacggtgg agaacctgag cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc
     2521 gctgacaacg gggattgagg cgagatcact aagccgagat gatcctcaag gttaagtact
     2581 gaaaggctga agaacatgaa cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg
     2641 cctacgtgat ctgacaggcc gtgcaggagg aactggcagt gatacgtaag cactgccggt
     2701 cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca gcgtctatca cgcttgcgtt
     2761 gctgacttct gccaacttgc ggcaagcaag gataaagagt gcgacgggca gcctcctcag
     2821 tatgcctgag tccaggcagg taaaccgggg aaggtcagcg acggatgtta agggggcatg
     2881 gctccgatga cgcgctgact ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat
     2941 tacatggcga agggaagcag tttgaatgtg tttgcgacgt gaattaactg gcctaacgag
     3001 gtgaagacct ttgataatca gcgaaatgca acgcaagctt gccacatggg cagccaccga
     3061 tccgtcccta cggattcaac ggctgctgcg tctgataaca caaccagaat ggctggctga
     3121 agcggcgcgg atcacgcttt catcaaaggg ggcgcatacc cccggcgttg atggcgtgaa
     3181 caaaacaatg ctacaggcca gactggctgt tgagctgcaa atcctcaggg atgaattact
     3241 ctcaggccac taccagccct tgcccgccag acgggtttac atccctaaaa gcaacggcaa
     3301 actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat
     3361 ggcgatggag ccgatatggg agagtgattt tcatacgctc tcatatggct tccggcctga
     3421 gcgcagtgtc caccacgcga tccgcacggt gaaattacag ctcacagact gcggtgaaac
     3481 ccggggacgc tgggtgattg aaggcgacct gtccagttac ttcgacaccg tacatcatcg
     3541 actgctgatg aaggctgtac gccgcaggat cagtgacgca cgtttcatga ctctgctgtg
     3601 gaaaaccatc aaggcgggac atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt
     3661 gccacagggc ggtgttatat cgccgctatt atcgaacatc atgctgaatg agttcgatca
     3721 atacctgcat gagcgctacc tgagcgggaa agccagaaaa gatcggtggt actggaataa
     3781 cagtatccaa cggggccgaa gtacggcggt cagagaaaac tggcagtgga aacccgcggt
     3841 ggcgtactgc cgctatgccg atgattttgt cctcatcgtc aaaggcacca aagcacaggc
     3901 ggaagccatc agggaggagt gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa
     3961 catggataag actaaaatca cccatgttaa tgacggcttt atctttctgg ggcacaggat
     4021 cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa
     4081 agccagaaac ttcgccgcat cgctgacagc actgttatca ggcaactaca gtgaaagcaa
     4141 agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc tgggccatgt tctatcagtt
     4201 cgttgatttt aaggccaaag tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc
     4261 tcactggctg gcccgcaaat accgtacagg tatcgcttcc ctgatgaggt ggtggtgtaa
     4321 atcaccggaa ccgggtcaga gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa
     4381 gctcagcggc gaaatactgt accggttggt ggggcaaggc aagaagctgt tccgctggcg
     4441 gctacccgaa ggtaatccct atctgaggac ggagaccaga aacacgtata catcgcgctt
     4501 tacagaagtg gcaatggcgt tcgccagcat ttaaatggag agccggatgc gctgaaaggt
     4561 gcacgtccgg ttcggggagg agaggcaggg aaatagtccg actacgccct gcctcttact
     4621 ctactctgga cgggtaagcg ctgggcgtac ctcgccgttg ttctcgacct gttcgcaaga
     4681 aaaccagtgg gctgggccat ctcgttctcg ccggacagca ggcttaccat gaaagcactg
     4741 gaaatggcat gggaaacccg tggtaagccc gtcggggtga tgttccaagc gatcaaggca
     4801 gtcattatac gagcaggcag ttccggcagt tactgtggcg ataccagatc agacagagta
     4861 tgagccggcg tggaaactgc tgggataaca gcccaatgga gcgcttcttc aggagtctga
     4921 agaactaatg ggtgccagcg acgggctatg taagcttcag cgatgcagct cacgcaataa
     4981 cggactatat cgttggatat tacagcgcac taagaccgca cgaatataat ggtgggttac
     5041 caccaaacga atcagaaaac cgatactgga aaaactctaa cgcggaggcc agttttagtt
     5101 gaccacaaca gactacctga agggagccgc ggtcgcctag cagttgcagt agcaggagat
     5161 catccagccg cagtacaggt cacgatgact ctggttaatg ataccggctt tgaccccgta
     5221 ttttccggct ctatcgctga atcatggcgt cagcagccgt gcacaccatc ctattgttgt
     5281 gactgggagg ctgccaccat gcttcgcgct ttccctctgg cgaaaaaggg agaaggacgg
     5341 gcccgtctgc cttcacttta tgccagcttc ggtaagctgg gtgagacacc gactcatgaa
     5401 gatatcattg ataacaatcg atccatcaac tggcctgtat aacgtggctg ccggtgatta
     5461 agaaagctgc acctacctaa gtagtagcaa acgcacactt tttagaaaaa tcgatggtca
     5521 gaaactggat tagcaattcc gttccagggt tgcttttgat ttacgttggc gtctgatcat
     5581 tgatttatcc tcaaaagccc aacctcattg gtaatgaacc agctccgtga atgtccgctc
     5641 tggcacagag cgaaattttt tgatctcccc ccctgaaatc taaacttagt catgtcacgt
     5701 ttttgggttt ctaaaatttt aacttcgcgt ttttcgttgc cgtaagggtt atacagaaat
     5761 gtccgttaag cagagttcaa aattgattgc cgtgatcacg actggtttga aagccgcgcc
     5821 caagcctgta cagctctggt ttgcgttgat tatgaacctg tcagcctaaa gcaagcggat
     5881 ggacgatgag tattggtaat ctttcagagt ccggaaaagt tcagccccag tctgaacagg
     5941 cttgctggcg ccagtccagt ttcattcagt cgtggtttgg ttcttacggc ctgtgcaatc
     6001 tacctcatta ggcacatcgg cctgccagat accggctcgg ggtgtatttc cgcttccacg
     6061 ctgaatactg ttctcagcaa tcctggggtc atcacctctt ctggtgtgcc ttgcgccata
     6121 acatgtccgt ttgccattac caccagttga tcgcagtacc ggctagcctg attaaggtcg
     6181 tgcagcacag cgaccaccgt tttcccctga gtccggagtt cgcccatcaa ccgcatcagg
     6241 tccacctggt gattgatatc aagataggtg gttggctcat caagtaatac aacgggcgta
     6301 ttctgggcca ggaccatcgc cagaaatgcg cgctggcgct gaccgccgga aagctcggtt
     6361 aaccgacgaa cggcaagatg attgatccgg gtctggttca tggcgacatt aactcgtgca
     6421 ttgtcttcag cggagagacg cccccagagt gacagccagg gattacgacc atacgaaacc
     6481 agctcctgga ctgtgatccc ctctggcgtt aaatggtgct gaggcagcag cgaaagcctg
     6541 cgggccaact ggcgtgatga gagcatattt atgggattat cgccgagaaa tacggtgcca
     6601 gactgcggca ttaaaagccg cgaaaaacag tttaacagcg tcgatttccc gcaaccgtta
     6661 gggccgatca gggcggtgat cttccccgtt ggcagtgaga gtgaaacgtc gttaagtacc
     6721 ttgtctgtcc cgtaactgac cgtcagattt tcagttcgta aagtcattta tcgcattctc
     6781 acaagcaacc agacaaacca cggcgcaccg ataatggcgg tcagcacgcc aaccgggagc
     6841 tccagtgggg gatgaataat tctcgccagc agatcggcaa ccaccaacag caacgcacct
     6901 gtcagggctg aaacaggcag cagtctgcgg tgacgtccac cggtgatgct acgcatcata
     6961 tgcggcacca cgagaccaat aaagctaatc gggccgcagg cggccacgcc ggtagatgtc
     7021 atggcgacag ctagtaacaa agcccagaat cgggtatggg gcaccgacac accgagcgtg
     7081 gtggcgcgcg catcgccgag tgcaaggagg tcgagatcgc ggcaaaaact caggctcagc
     7141 ggcagaaata aaatcatcag cgggatggca atcttcacaa agctccagtc acggccccat
     7201 aagctgccgg tcagccacag cagggcgttg ttcacatcct gcgggcgcga gagcatcaga
     7261 taatccgtca ggctggccca gcatgcagaa agcgccacgc cggtgagcgc cagcttcatc
     7321 ggctggtggg tctttgccag catcttcagt aatatcaacc ccgccatgcc gcccgcaaag
     7381 gccagcagcg gcagcaccat cacgggcagt gacggcataa gaagtagagc ccccacagag
     7441 gccaggctgg cggcatggtt aacgccgaga atatccggtg atgccagagg gttgcgcaca
     7501 atcccctgta tcagcacgcc cgccacggcg agggctgcac cgacaaacag tgccagcagc
     7561 aagcgcggca gtcggtactc catcaataca taataatgct cgcgtccggc ctgccagtcg
     7621 gtcagcagcg cgcgccacgg cacggggatc actcccatat ggagtgataa cagcgcacag
     7681 cccgccaggg caagggtgat gaaaataacc agcgcaattt tcatcctcgc ctcctcacaa
     7741 gccagacaaa gcaagggctg ccaatcagcg ccagcactgc gcctgcgggc agatctccgg
     7801 ggaaggccag cgcgcgtgcg agtacatctg ccagcagcat cagcgtggcc cccagcagca
     7861 tgctcactgg cagtacgttg cgctgatcga agcctgccca gaagcgcgcc agatgtggca
     7921 ccagcagacc gataaacgcc accggacctg ccacgctgac gcacgcacca accagaagca
     7981 gcactaacat attgatgacc aaacgtagcc tcgtcaggtt cactcccagc gtatgggcgg
     8041 tgctgtcgct gaggttgagc aggttcagtt gattcgccag cagtaacacg acagggactg
     8101 cagtgaccac caccggcaag agctgccaga catcctgcca gcgggcgtgg gacactccgc
     8161 ctgccagcca gtaaaagatg ccgtaagcat gatcttcggc cagcagcagg gtgatgcggg
     8221 taaggcccat acaaaaggcc gacagcgcga tacccgcgag gatcagtttg tttctgtcat
     8281 gggtatgacg aaatccgcct cctgcggtca tgaccagcag ccagctcacg ccgcccccgc
     8341 atgccgcgat gaacgacaga gaatagcctg caatcggcgt cggactcagc gcgctggtaa
     8401 gcgccatagc cagcgccgcg ccgctgttaa tgccgagcag tgaaggagag gccattgggt
     8461 tgtgggtcag ggtttgcagc agcgtgcccg cgagcgccag gcttgcgccg atcagaacgg
     8521 cgaccaggct tcgtggcaaa cgaaggtttt gcaccagcgc ttctggtagc gttggcgtgt
     8581 gtccaggcag cagggcgcgg gttgcatctg ctccggaaac aggaatggcc gagtagcaaa
     8641 acagactcag ccagaaaata ataataagtg ctgcaacggg aagcccccac agcagcatcg
     8701 ggtgttttat cgcggtcatt tcacaacggt aagcggctga tggtggaaga tttttaccgt
     8761 gtcagcggca atacgctctg cagcaaaaat accgcgcatc cgcgcccagg tgttactgtc
     8821 gaccgaagca acctgctgct tctgcgcggc ggttaacatc tgccagagcg gatcttgttg
     8881 ccagcgttta acaatgctct cttcgcgata gtgggcaacc agcagccagg caggattgac
     8941 cgccagcagt tgctccaggc cgatggacgg catggacgaa cccgccatcg cagcgggaac
     9001 gttcagcccc agagaggcca gcacgctgcc ggtccaggtc tcctgagtat gcaggttgaa
     9061 ttgctgttcg cgtgatgtgc caaaggccac gcgtgtccct ttgggaagct ggctggccca
     9121 ctgcgccatc ctctctttat gttgttccag acgtgcctgc atctctcgct ttttacccac
     9181 catttcgccg atgatagccg cagattgcaa attttcagcg taggtttcgt tgcgggactt
     9241 aagcagcagt accggcgcga tttgctgcaa ggcgatgtaa acccccgcat ggcgactgct
     9301 gtcggcaatg atcaggtctg gtttcagagc ggcaatggct tccaggctcg gctgcgcgcg
     9361 cgttccgacg gactgccacg gtttcaggtg cgcacgcact tcgggcagga tgcgttttgc
     9421 atcgttatcg tcggcaatac cgatcgggct gacgtccacg gcggccagcg catcggcgaa
     9481 cgagagttcc agcaccacaa tccgttgtgg cgttttttcg agtgtaaacg tgccgtgttc
     9541 gtcctgaacc atggcggcaa aggcgtggct gatcaccagc agcaggcctg caaaaagaaa
     9601 acggataaat gccaacataa tcacattcca gctaaaagcc cggcaagccg ggcgttaaca
     9661 catcagaact tcaacgaccc ctgcatatac agcgtgcgcg gctggcctgc atagatgcct
     9721 ttgttgttgt cgtcataaga gcggatgaag tagtcctggt cgaagatgtt tttcacaccg
     9781 aatgccaggt tcagatctgc catctgcggg ccaaagtcat acgccacgcg tgcaccccag
     9841 agcatgaagc cgggaatgcg gccggtactg ccgtcggcgc tctctttcac cgtattggcg
     9901 ttatccgcaa actggctgga ctggaaatcg ctgttcagat tgaacgtcca gtttcctggc
     9961 ttgtagtcca cgcccagcgt gcctttatgt ttcggggaga atggtaccag attgccgtag
    10021 gtgtcgcctt tctcgcggat ttccgcgttc acatacgcat agctggcgta gatggaaacg
    10081 ttatcaagcg ttggcgttag cgtacccaga tcgtaacgtg cctgcgtttc cagcccggta
    10141 tggcgcgttt tgccacgtgc agtgacggtg tcgttggtct ggttggagtc gtactgattg
    10201 ttaaagttaa tcaggaacag ccccatttcc gccgtcagcg cgccgtcgtc gtagcgggta
    10261 ccgagttccc aggttcgcgc tttttccggt tccacattgc cgctttgcac agccttgcca
    10321 atctggctgt actgtacggt gccgaacgag ccttcagtgt ttgcataaag attccagctg
    10381 tcagtcaggt gatagagcac gttcaacgcc ggaagcggtg cgttatagct cacttcttcg
    10441 tgcgtgcctg tgatggcgtt gttctggtat gactcgatat gttcgaaacg catacccggc
    10501 gtgatggtcc agttgccgat gtcgattttg tcatccagat accaggcgtg cgcctcggtg
    10561 ccggaacgcg tatcgcggtc gtaagggctt gagccggacg gcaactgccc gctgctggtg
    10621 gcggtgtagt aacgcatttc atgcgttgat tcattcacat agcgatagcc cacgcccact
    10681 tcgtgcgcgg aagggccgat cataaagatc tggctgtagc gtggctcaat accgcgcacc
    10741 cagtagttac gcggcgagag ggtgatgcgt ttgccttgct ccaggtagcc gctgcgcagg
    10801 gtttgggtgt agaacccctg aatgttgaat ttatgctggc tgtctggctg gaactggtag
    10861 cccaggctcg ccagcttgcg acgaccccag aagcggtcat acgggcgggt ggattgccag
    10921 cgatcggcgt cgtaatccgc gcgagacagg ccaccgggca tgtcggcttc accgtcgtaa
    10981 tattgcagca ggctgttgaa ggtgtgcacc tcatccggcg catatttgct tttcagcatc
    11041 aggtcgtcga tgcgggtggc gctgtgctcg cgccagtcac tgccgcgcgt gccggagtag
    11101 agcagcgcgg tgccaaaacc gttgtccgct gtgccgccca ccatcaggtt gtgcgtctct
    11161 ttcgggttgt tttgtgaaga ggttgggctg agctgacctt ccacgcccgc ctcgatacca
    11221 aagtcctgcg gaatggcacg ggtaacaaag ttcaccacgc cgcccacgct ctgcggtccg
    11281 taacgcaccg caccaccacc gcgtaccacg tcaatggcat ccatgttgcc gagcgaaatg
    11341 ggagccagtg aaagctgcgg ctgaccgtaa ggggcgaagg ggacggggat gccgtccatc
    11401 aggacggtcg agcggctggc gaggcgcggg ttcaggcccc ggatgccaaa gttcatcgcc
    11461 aggtcgtggc tgccggtgcc gttgttttcc ggcgcgctga cgccagggat gcggttaagt
    11521 acctcacgca tggtggttgc gccggttttg gcgaaatcct cacggcggat cacgtcacgc
    11581 gcgccagcat gttcaaatac gtcgttttca cgcgcatcac ccagccagtc gccgaccacg
    11641 gtcagggcat cttcttttgg tgcgggcgcg ggctccagcg tccagctgtt atttcccagc
    11701 ggttttacct gcagtccgct gccgtccagc agttgttgca ggccgctctc gacgtcgtaa
    11761 tcgccgtgca ggccgttgct ctgcttgccg cgcgtcaggc tggcgtcaac cgagagggta
    11821 aatccgctgt gtgcggcata ctgattgagc gctttatcga gcgatcccgg tgcgatatta
    11881 acctgtgcag caaaagcgga aaacgagaga ccggccagcg gcagcaggct caggcgaatg
    11941 gcgttaacca aaggtgttgt tttacgaaaa acgcgtaacg gcgtcatacc ttccccatca
    12001 tcatttttgt tgtgttcagc tatgagtcga acgagaaggg taaaaaggac aatcgaaata
    12061 agaattattt tccttacagt ggtgaaatgt ttatccagta ccgcgtaata gactgaattt
    12121 taacgggaag cgtttgcgcg ataacgttca ggatcgcatc ggtatttttc agcgggaacg
    12181 tcccgctcag gcgcagcccg gcaacggcgg gatcgcagcg cagcacgccg ttgcggtaac
    12241 gggttagcgt ggctatcacc tcacccagcg gtttatcgct gaagctcagg atgtccttcg
    12301 tccagcttgt actctcgtca tccagcggtt tcactgcgcc aaactcagag gcgctgaact
    12361 gcaggctttc accagcgttc acgatgcgtt tttgcgcggg ggcactggcg agaagcactt
    12421 ccacagcgtg ctgctgaacg tcaagctgcg tgaaattatc ctgctggcgg acggtaaatt
    12481 ctgtccctaa agcagtgagc tggccctgac gggtcaggac acggaagggg cgttgcaggg
    12541 catctttcgc ggtagtaatg gcgatttcac cgtaccagag ccggacggtg cgctgatgcg
    12601 catcaaaacg cacatccgcc gcgctttggg tattcagcgt gagcagggag ccatcttcca
    12661 gttgctgacg gctgactgtg cctttggcgg tgcggtaatc tgcccgcaga ccttcgccgg
    12721 tttccgactg ccagagctgc cagcctccgc cagcgccgag caacagcagt aatcctttca
    12781 tcacgtgacg gcgggtgagg cgggtatcgt gcaacgcccg gctggcaacg tcgccaggca
    12841 caccgccaag ctggttgcgc aggttttcaa cctgctgcca ggcccactgg ttatcctgat
    12901 cctgttcata ccactgttgc cagcgcgctt cctgttgtgg gctgacgcgc tcgccgctta
    12961 gcacggcata ccagtgggaa gctgaacgca gcgcctgacg gcgggaatcg gttaacaaag
    13021 gattcataac ccatactcca gacggaacag caggcagtgc tcgacggctt tcgccacgta
    13081 ttttttcacg gagctgatgg aaacaccgag tttgtgcgca atctcgctgt atgtcagacc
    13141 atccagttgc gaaagcagaa acgcttcacg tgttttgccg tttagcccgt ccagcatgct
    13201 gtcgaggagt tgtagggtct cgagttggct ttcgcgttcc tcaggtgaag gcgctccccc
    13261 ctccggcata agcgccagca tctccagata cgctttttcc agcgcgtttc ggcgaaacag
    13321 gtccaccatc acgcgtttgg cgatagtgca gaggaaggag cgaggatcgc ggatcgtcga
    13381 gagcgtttcg ctgaccatta cccgcaaaaa agtgtcctgg gcaatgtcat ctgcatcaaa
    13441 agcagactgg agtttgcgcg tcagccagct tttcaaccag ccgtgatgtg tgccataaag
    13501 cgactcgaac gttaaggaag ctgtggtagt ggcgcggtca gacatgcgga gtgcatcaaa
    13561 agttaattat cacgtagtca tattaatatg agaatggtta tcattacaat tggaaataaa
    13621 attgtttcca atagacattt ttaacatgtt gtttttctaa gtgttataag gtaggtataa
    13681 aatgggatgg agcctctgct tctgacatgt gtcggtcaga atgactcatg atgtggtctg
    13741 ctattattga catcctcact gccctaaagg atggggattt cggtaatgat cttacccagc
    13801 aatagtggac acgcggctaa gtgagtaaac tctcagtcag aggtgactca caagacaaaa
    13861 cagtttcaac cagtaaaaaa acgtaaacac cattcgcatg aatttcgtag ttaagatctg
    13921 aagcttgcag aatgcatcgg tgttactgcc ggagtccgtg aacttagtct gcatgaatca
    13981 ccacagcttt acatctgtcg cagtaaacag caaaatcagc aaacttactt aattatatcg
    14041 agtgcgatta caatcgctgg cgatggtaca gttggtgtgg cggtctcagt ccggaacaat
    14101 ttgaaaatca taatctcact taaaacggca tccattttac aaaagatggc cgtctgtgta
    14161 tttgtaatga ataccttcta catgtttaga gtgcataatc cacgacaagg cgatatccta
    14221 aaaattagct agcaggcaac tttgatggca tagataatgt ttttagtaaa aaagagttga
    14281 ctgaatataa tacagcctct tcctacataa gaaggaatgt gctcacaaaa aaaaaccatt
    14341 agtctgcttc ctgttcttac aataatcagt attatgtatt ttatttatat agcaaaatat
    14401 gcttgttaca cctgtcataa ttaggcatgg taaaattatt gactgaaccc tgggggagaa
    14461 agatgcacag tgattattat aatatggttt ttggtgaaaa attggccaat attttatatg
    14521 aagctaactc tcaatttttt tacgagagaa atgtcataga agaggcagtc aacgctttat
    14581 tctgtgagcg tgaaataata aacaacaaga acataataaa gaagctgatg ttctttttgt
    14641 cagatgttaa tcacaccaag aaagatgttg ttcagagcgc acttaatatt atcattgaca
    14701 ttacatcagg tgatatttga gattttacat aaaagataat ctctcaagga gtcttatctg
    14761 aaaacgaaat atatagtccc tctcgttttg taatatcgat tggctttgtt gaatcggtta
    14821 cgtaccccgg agccggtcat attttcaggt aatacacacg ctttcagaca tttcggcttt
    14881 cgtcattttt gtatcatggg catagcttct gctacctgac catcataatc acgcagcttc
    14941 agtgaagcag tgaccacctg tttaacccgg tgtatcgtta tgatcttatc cagcaattga
    15001 accgccccgg gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt
    15061 tccgatggaa gcataataag ctttttctgc ttctgccggt gggatatggc cctgcctttc
    15121 cagcaatcgt cgattgttat accagtccac ccacgtgagt gtggtcagct catcatactg
    15181 acacgtgcat tacccctacc gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg
    15241 cagccctcat cactccaatc caacaagaca cagcattttt gatcacgaag aaagacaaat
    15301 aatttccgta aatgtttccg ttgttacaaa taacaacagt gttttacatt tctttggcct
    15361 caggcccgcg catgttgcca ttcgatgtca ttttcctgac tcgtttcttc tttatggtgt
    15421 agtgtgatct tcactttata gatgctaaca atatgaaaag aaaaattatt cccgtgctaa
    15481 tcggttgtac gctctctttc tctgccctgg cggcacaacc tacggctgag cgctatgtcg
    15541 tcagttttcc agaaggcacg cacgtgaact acgccggcgc atttgccagt gcattcccga
    15601 acggccttcc ggttgggata ggttctggcc tgctgttcac cggtaaacag ggcgatgccc
    15661 tgacattcgc aaccattacc gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa
    15721 acgaaactaa aattttcgtt acgccagatt tcgcgccgct gctgatgacg attcgtgtac
    15781 aaaacggcaa agcggaggcc atcgatcccc gtcctctgca tgacgataaa ggcgcgatca
    15841 acggtctgcc gctggcaagc gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca
    15901 cgttacatag actgaagggg gataatcgcg ggctggatac ggaagggatc actccggatg
    15961 gtaaaggagg ttactggctg tgcgatgagt atggcccgtt cctgattaac atcgacagta
    16021 aagggaaaat cctggcaatc cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg
    16081 gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc
    16141 gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac gctggatatc gacgctaaga
    16201 gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg
    16261 cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg
    16321 gagacatcgt ggcgctcgat aaccagcaca tcctgctgat cgaacaggga agagataaaa
    16381 acaacagaat gcgtaacctg atttacgagg tcgatctcaa taaggcgagc gacctgtcag
    16441 gcttcgacaa gccgggtgag tatccggagt ttgatgatga gaaaaccctg tcacagcgcg
    16501 gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg ctccttgggc tggcagcagg
    16561 agaaggctga agggttggcg ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg
    16621 actttggcgt gaaggtggcg atgcaacatc cggtcgaggg caagacattc aaggattatc
    16681 gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca ggttgaaacg accctcagag
    16741 tgaagccact ggagaaaccg gagtctgaca gtgaactgtg gattgtgacg ctgccggaag
    16801 cgttgaaata agtttataag gtacggacag cagtatcgct tagtttttca aattcatgta
    16861 tgtgaacaat attggggttc cggcgtagac ccccaatgaa agcgtcacca cgtgaaaaca
    16921 tgcctgcacg gtttacacgt catcccatgg tggcagccgg ttaactttcc tgcgccacca
    16981 gtggaaaaag gccataagca gtccgaacaa aagtaagcgt acagcgaggg ccgtattgac
    17041 ggggatgtgt tattcagctg gcagtgctat gcgccacgga agcagttcgc tgacccggtt
    17101 gaccggccag tctgctatga cgccaagcac atggcgaagg tagctttctg gatccacgtc
    17161 attcagtttg cacgtcccga tcaggctgta cagtagcgct ccccgctcac caccatggcc
    17221 agagccgaag aacagggtct tcatcttttc acgcaaccag ctttccaggg atttcaacag
    17281 cggtttcgtt tttcgctgac gttcagcaag ccgctgctct gccggcattc cccttatatc
    17341 cgcctctatg gcgtacaact gaccgatctg ctccagggct tcttccgtca gtgctgacgg
    17401 gatgcggacg tgcacatcgt ggatctttcg gcgggcatga gcccagcagg cagcttccgt
    17461 tatcccacca ttgcgataca gctcgttgaa cccggcgtac gcatccgctt gcagcacacc
    17521 gctgaagcaa gcaagatgag tctgcggatg gatgcttttt ctgtccgggc tgtaagcgaa
    17581 ccacactgca ggtgccaacg ctgacccggc attgcggtca tcacgaacat acgcccacaa
    17641 ccgcccggtc ttcgtcttct tattacccgg cagcagtacc tggaccgggg tatcatcggc
    17701 atggagtttg ccgtcagtca tgacatagcc atgaagcgcc tcttccagcg gagacagcag
    17761 ccggcagcat gcatccaccc agcccgacag cagtgaacgg ctcagctcca caccttgccg
    17821 gccgtatatt tctgactggc aatacagcgg ggtgtgctct gcatacttcg aggtcagcac
    17881 gcgggccagc agccccggtc cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgc
    17941 ctgcacgatg gtatcgcact gagtacaggc atgtttttcc cgtaccgtcc ggataacccg
    18001 gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc tcgcccagat agctcagtga
    18061 accgccgcag ttcgggcagc acggcgccgc aggcaacagt cgcttttcgt cacggggtag
    18121 tgattcaggg aacggcttac gggtgcgggt ctgacgcaac ggacgctgta ctgccgggtc
    18181 atacacccta ccagtcagcg tatcgctctc tttctgaagc cggttcagat cggcttccat
    18241 ttgtgcgata cggcgggaga ctttttcgga acgactgccg aagttcatcc ggcggagttt
    18301 atccagctgc gcctgcagat ggtctatttc gcgctcccgg ttgctcagct tttcctgcag
    18361 ggcgtggatc agcgcttcct gttcggccag gcgctgtttc agcaggaaga tgtcgtcaga
    18421 agagatgtcg ttcataagcc cgtattttac cgggcttatt ctgtgacaac caggataaag
    18481 agatttacag catggtcagg gaggtcagca gccgcttggg ctgtcgccag tcgatacctt
    18541 ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt gccatcacgg gctgacggcc
    18601 aggcgaagcg cccacgctcc agccgtttgg tcaggaggca cagtccgtcg ccggtggacc
    18661 acagcagttt aacctgactg ccgctgcggc cccggaaaat gaaaatatgg ccggacatgg
    18721 gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc gttgaagcta tttctcatat
    18781 cggtgatacc ggcaaccagc caaattttgg tcccggaagg taacgggatc atcgcttcag
    18841 ttcctgtatc agcagagtca ggagcttttc gctgacattg ccattgaagc ggagcgtccc
    18901 gtgccggaac gttacctcac agctgatact gagggtttcc gggtcctctg cgagcgattc
    18961 tggctgttcg gcagctgcat cgagagtcac aggaagtagc tgggggctct ctgaagaagg
    19021 taatagcagc tttccctcgc gccattgttg tcgccatttg aacaacagat tggcgttaat
    19081 gccattttca agagcaagtt ttgagatgga tatcccgggt tcacaggagg cagcaacgag
    19141 cagctgttta aattcgggag gataattagg gcagcctttt cgcctgccgg gagtcacatt
    19201 tttctgcata tctgacactt tggttcccac tacttatttg gtggacacca ctttgtctaa
    19261 ttcgtcagat tctgaccaga cggttcaggc tgtacgctta ccactatttt atccctgttg
    19321 aatttggtta agccggtgca gacgcgccgg gaaacgcaca cctgaaccgg cacaataaaa
    19381 tacaatacaa ccccctgtta ctggagatac gaatggaatg acgaggcagc ctgtcagtca
    19441 cagggggctg ttggcataaa atcaacggaa aggccaccaa caccggaatt aagctccaca
    19501 gtccccgcac tggcaatcca ttcccgggaa aggcatcacc gcaaactgct ccggaggcaa
    19561 ccattacagc ctctgtaacg gattatcagc cctggcatct tccgacggca cctcctgccg
    19621 cccccacgcc ctgctgtata ccccggtacc gcagcgtggg cagcactttt ccccctcata
    19681 atcttcgtca cacatcacac aaaaccactt tctcaccttc accgtataat ccggaaccgt
    19741 catgccctgc agatgcaaac gacgggcctc tttctctgcc gggtcaagcc aggtacatcg
    19801 cagcaggatt ttttcagcga cagtcatctg gtcatgttca aaccacggtg gttgtccgtc
    19861 attgccccgc tgaagtgtta acgggcatcc acaggacaca cagcacaaaa cgtcctgtcg
    19921 gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct ttgctgtcat tcgccatata
    19981 acagttcaga attcgcatat ttcctcctga gttgtccggg gaatactgaa tcctccggtc
    20041 tggcagtgtg tgcagtactg ctccccataa taatcgtggt gacattgtct gcagtgccag
    20101 ctggctttac gcaccacggg taaggcatcc ggtacgaatt gctgcagacg tttaatcaac
    20161 tgtatttctc tgcgctccgg cctgacataa gggcactgtt gaccgtgctc tgtcagcctg
    20221 tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt gcggatggta tctgagtgca
    20281 ctgccacaca agtggcaggt gtagttagca taaggtgcgg cctgtgcagt acgggcgccc
    20341 gtcagacgtc cgttgccatc aagagcgata aaggattttg cgtacatagt catatacctc
    20401 agccgccaga cgacacgcag gcaacagccg tcccggtacg ggcagcgtgg tcagggtgtg
    20461 aatggcggtt cagttaaaat ctggactgga gtttgggtaa agcaggctgg ttgctgttac
    20521 agcactgtgg ttgtcacaca atggaacact gacgtaaaaa acagtcagtg ggcgagtgga
    20581 tacatcgttg ccgccagaca acaacccata cttttggttt gccggatatt ggcgcgccag
    20641 tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg gtgcagtgag ctttggtatg
    20701 ctccttcgcc agatagtcag cacgttccag cacttgctga aagccggtgt catcattacg
    20761 cgccagccac accgccggcc aggccggaaa atgcaccagc gtggcatggc acccgacatc
    20821 cactcccagg gcactgcacc acgcctgttt aatcatcccg gccagagact ccggagcgcg
    20881 gtaatcaccg gcacgacacc aggtatcccg gttgaccagc agcatcaggt gatagtgttt
    20941 tttacctttg cactccccaa actcccgggc ccagacgtaa tgcagggttg tgggatgcac
    21001 gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg attcgggctt tcagggcatt
    21061 gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt acatccggga gacgcaggtc
    21121 aacccgcaat gccgtcaggc ggggatgaac attcagtgcg tgccgcaccg tctcacgaat
    21181 acgttgctgc cagaaggggt tgtatttgta ggtcatggtt aaatctccgt atggttcata
    21241 cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa gtgacctccg gcaggacact
    21301 gtctcaggca ctctgtgata acccgaacgg tcgtctccgg actgacggtt gaattttcag
    21361 cgtcgttttt taatgtaaaa aaccggttac gggtaaggct gtgaggaggg agtgatggtg
    21421 atacgcaaaa agaagtgcag ggactgtgga aacgcgatta ctcataatac agtgtgttgc
    21481 ccgtattgtg gttctgtcga ccccttcggc tactaccgga atacagaccg tatcgttacc
    21541 atactgctgg cgctgattat tgtggtcctg ctgactactg tcagcgtcag tgtgtgtata
    21601 ctgtgtagct ggtgagataa cggttcatgt atcccctgag ctgacagtgt accgtgtata
    21661 ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa ttttccaggg attgccgctg
    21721 ccttatttcg tcccatacag gaaaactcat ctgggtttct gacaaatacg cgaaactcac
    21781 agcccctcaa tatcaaccac acgttgtaag ccggtaatac ctgaaaatca gcctattacc
    21841 gcccacgcct ccggcaccat tcccttttga tttcgaattc tttgttgtcg ctataccgct
    21901 taatgttccc ggtcctccgg tgcagataac accggagtca tcgtagcgct gcttcaatat
    21961 tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg taaagtttgt tcattgtcag
    22021 ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc agctccgcaa gggtaagttt
    22081 ataacgcgtg tgggaattca cttttcccgc gtctcctgac gtattacggg ctgccactta
    22141 cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg ggatccggac gcaccgcaac
    22201 aaccggggaa gtatttctgg tttagagcag tgtcaagagg gatttctcag cccctcactc
    22261 accggctcag tctcagggca ggagcattaa tacgctcagt tcatgtctta tacaggaatg
    22321 ggcacagggg gagctggcgt gtcactgctg attttacgca tcagcgttca tgtatctgca
    22381 ctctgataca tgaacgattc acagcgctct gttaccggag agatttactc tcttagctta
    22441 ctctggcaaa tcctttacgt tactctctga tgacttctta tatttataat accaatcaga
    22501 cagccttatc ccatcggtga tatcagtctc ctcctcgcca ggaacgggct gactcactgt
    22561 aactacctcc gaaaacaact ctgcaattat cagaacgcct gcttctctcc ctgtcctcac
    22621 gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca tcttttcgca acaaccaccc
    22681 ggtataccgg tacggctcag ccaccgcaac ctccccgttt atctcctggc ggtcacattc
    22741 ccgattattt tttacgtttc tccccggttg ttatgccggt gaaggtggcg cgctgttttc
    22801 atgaccacac cggctggttt aacaacatcc ggaggaacat tctcatggct accccagttt
    22861 cactgatgga tgaccagatg gtcgacatgg catttatcac tcaactgacc ggcctgacag
    22921 ataagtggtt ttacaggctc atcagggatg gggcctttcc ggctcccatc aagctgggcc
    22981 gcagctcccg ctggctgaaa agtgaagtgg aagcctggct acaggcgcgt attgcacagc
    23041 cccgcccgta atttctgacc gttatccgtt cacccgcagc agacgtaatc cccggcctgc
    23101 cggcggcatt ctgctgcctg ttatatcccc gtgaggaata tgaaaatgaa acaacagtat
    23161 cagacccgct acgaatggct ccacgaaagc taccagaaat ggctgaccgg cttcacccgg
    23221 cacgccgtat cctggggcgt gtgtcatccg aatatctact acttccataa tctgacgccc
    23281 ggttgggggt cattcaatgg tgaaaaaccg gagattgcca tcgtacccca gagcctgcac
    23341 cggctgattt atggccctga caaacgggcc acgccgcccc tggatgatga tctgatagtg
    23401 aatttatgca ccagtgaaca tctgctggtt catcatccga tgctggaagg cattctcctg
    23461 tctgaatgtg aacgtctgag gcagcgttct ctggcaaata aactgatcag cctcttccgt
    23521 cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc tttgctggct tgatttaatg
    23581 accggaaaca gccttgaaga ctggaaagag aacctgaaac ggaaatcaga aaaagagctg
    23641 gaggaatgga tcatcaaccg tcagaggcag agcgcagcac tgacggatct gatggatcag
    23701 tacgtgctcc tggcataccg cacaacggtt gacgatagcc gcaactgatg ccggttgctt
    23761 ccggaaggtg tgacaaaata cggaaatgat gatgctcccg gcccccatgc tcacctgcag
    23821 agcgtgttcg tcgtcatcat actgagccat gacgggcgtc ttaagggcat cctgtcattg
    23881 ctgagcaggc aacaccacca gaggagccac ggacagggat gttctttaca gcccgataca
    23941 cgccaggttc tcagcagact gaatgtgcag gtgcggttat tcaccagaaa tggcatgctg
    24001 cggtacatac ccatcctggt gacaatatgt acgtggcgac agcgatcccg gcactgtgga
    24061 agagactcac tcagcctgca ggatattctt tccggcatcc gggaatgtcc gcctgaatgc
    24121 aaacaggatg atatttgaat ccgtaatact gttctttcat cataaataat ttatgcagat
    24181 acagttaacc gttacagtag ccggtttgtt ttaccgggaa ataatatccg tcagactgat
    24241 accggaacat atctgaaaca cgttatgatg ttttgttatc agaaatgttg tatattctta
    24301 tccgtggcga cagtttaatg ccctctggta tcacctgata atgtatatcc tgcgatgatt
    24361 cgttatattt acgggacacg cctcttttca ttgtgctcct tttcagactt cattttccgt
    24421 aagtgttccc cggcataaag gaggtacctt tctggcgttt ataactctct ttatcggtga
    24481 cgccggtaat acagggaata ccacaccgac aaacgaggta ttaacagacg atatttcagt
    24541 gtccataatt tttctgtccg gttaaataac gtcagtctgc tgccctctga ctgtaatcat
    24601 caaagccgga atgcataccg ggttacaacg taaaaaaagt gatgctggcc cccggtgcag
    24661 ctggcgggcg aaacatgttt acctttcaat accccctgtc tgcagtctcc actcttccat
    24721 cctcttaagc tacgtcggcc acatgagtgc acttccatga taacaggtat tcctgtcagc
    24781 ataacgaagc cggttacctt cccttcctcc tgcatttcct gtcagaatga ctatacagcg
    24841 cgcagattgc ctttttattg accatcttaa tataccgtta taaacttctt tttatcatat
    24901 tattatcacc ttattatgtt taatttttaa gcagtcggag aaagaggatg gttcagaaaa
    24961 ttttgtcgga taaggttatg aatgaaagaa caaatgcgta ttactcttat tatcttggag
    25021 agcgtaatat ctccgtactg ccccttaatg tttatgatcc cccggaacgt tttatcgctt
    25081 acataaaaaa aaacagagaa aatctgaaca tcacactttc agattttgaa cttgaacaaa
    25141 taatttctgg tatgcgtctg aaggccctgg ccttcctggt ccccctggaa aaaatatcct
    25201 ggatcgcggg cagtgaacgc gcatgcttat tttcctggta tctgttgatg cagtttatcc
    25261 agaataacag ggcaaaaata agcgcagact tgcttcaaaa gaataaactg tatcttaaag
    25321 aagagtatct ggaagggaat gccttcccgt cggactcatc gacacagttc agacaaatac
    25381 tcagagttct cgatattctt tctgataaga atttgcggga tgagtggata atccagacca
    25441 aagacagatg gatgcgggca tttaaatcaa agagtccttt cagttacctc ctcccggaga
    25501 acgaacatga atgcatatgg acatggaatt atttgaaggg aaaaaatatt gcactggaaa
    25561 aactggccag ttttcctggt tctgctgata tttaccacgc aattcacctt tcatttgaca
    25621 tatgggttac ctgtccactc acctcacctg acgatataaa aaatttcaga aacagtttca
    25681 ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga agataaagtc aatgtacagt
    25741 tttttcttga cgcagaaacc agggcgcagc tcaaagaatt atcgagggtc agaagactca
    25801 gtaccggcga aatgctgcat gacctgattg ttgaagagta taaaaggtac cgacattcca
    25861 gataatttca gacatatatt atctccgtga gtcccccgcc acctttccgg tgtgcggggg
    25921 ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg
    25981 aaaaaccgct ctcctccctg ccttactcac tttcccgtca catcctggaa catctgcgca
    26041 aactcaccag tcacgaaccc gtgattggca ttatgggtaa aagcggggcc ggtaaatcct
    26101 cactctgtaa tgcactgttt cagggggagg tcaccccggt cagtgatgtt cacgccggca
    26161 cccgggaagt gcagcgtttc cgtctgagtg gccatggtca cagcatggtt atcactgacc
    26221 tgcccggggt gggcgagagc cgggacagag atgcagagta tgaagccctg taccgtgaca
    26281 ttctgcctga acttgacctg gtactgtggc tgattaaagc cgatgaccgt gccctgtctg
    26341 tggatgagta tttctggcga cacatcctgc accggggaca tcagcaggtg ctgtttgtgg
    26401 tgacgcaggc cgacaaaacg gagccctgcc atgaatggga tatggccggt attcagcctt
    26461 ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc ggtattccgt ctgttccggc
    26521 ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg ggaactggat acgctggtca
    26581 gtgcgctaat gacagcactt cccgaccatg ccgccagtcc cctgatgacc cgcctgcagg
    26641 acgggctgcg cacggaatct gtccggggtc aggcccgtga acagtttacc ggtgcggtgg
    26701 accggatatt tgacacggcg gagagcgtct gtgttgcctc tgttgcccgt aaggttctgc
    26761 gcgctgttcg tgacaccgtg gtctctgttg cccgcgcggt atggaactgg attttcttct
    26821 gaacccggtg gtgatgcacc agactcttgt tacagatttt taaccccatg aaaacacaat
    26881 gcaaacacag ccatacccgg gttgtcccct tgcgttgtgt ttctcaagtt aatagaatgc
    26941 atcgatcgtt actatcatat cgatcgctat aaacgatcgt ttgtggtaaa tgacctcctc
    27001 cgtggttgtc atgaaaatgc gtctcaggaa ggttaaataa tgcacgaaaa gaacatcgcc
    27061 ctgctttgtg atgaagccga ccgacttttg caactgaaca ttaatctgct ccggcaaatg
    27121 gttgatgaac cggatgtgtt atctgacagt aagaacgaaa acagactgct ttttgataaa
    27181 cagaaagcac tgaaaagaat tgaggagctg gagggcgaac aaatcaaaac cgcccgcagg
    27241 gagatggtgc tggctgttgt cggcacgatg aaagcaggta aatcaactac catcaacgcc
    27301 attgtggggc aggaaattct gcctaaccgt aaccgcccca tgacctctgt accgacgctc
    27361 atccgccacg ttcccggaaa aactgagccg gtcctccatc tggaacatat tcagcccgtc
    27421 cgcaatttat taagcacact gcaacaaaaa ctcgacaccc cggcaggaca gcaagtcgca
    27481 cagaccctgc agcaaaccgg ggatacctgc gaactgctgg atattctggc aaatgatggc
    27541 tggctcaaaa atgaatacca cggggaagac gaaatcttta ccggactggc atcgttaaac
    27601 gatctggttc gccttgctgc ggcaatgggg agtgagtttc cttttgatga atacgcagaa
    27661 gtgcagaaac tgccggtgat cgacgtggaa ttcagccatc tggtggggat ggatgcatgc
    27721 cagggaacac tcacactgct ggataccccc ggccctaatg aggccggaca accgcagatg
    27781 gaagtgatga tgcgggatca actgcagaaa gcctctgcgg tgctggcggt gatggattac
    27841 acccagatga actcaaaagc ggatgaagac gtccgtaaag agcttaatgc cattgctgac
    27901 gtatcagccg gccgcctgtt tgtgctggtc aataaatttg atgagaaaga ccgcaatggc
    27961 gatggggcag atgccgtacg ccagaaagtt ccggcaatgc tgaacagcga tgtgctgccc
    28021 gcctcccgcg tttatccagg ctcatcacgt caggcgtacc tggctaaccg tgcgcttcat
    28081 gagttacgga aaaacagaac ccttcctgtt gatgaagcct gggtcgatga ttttgtcagg
    28141 gaagccttcg gtcgcatgaa aaaagaatac gtctgtaaag acagtgaaat ggcaaccgaa
    28201 ggggcgacag acctgtggga aggctcactt atcgatcaac tgataacgga agtcatactg
    28261 aggtgtactg
//
LOCUS       DAJTNV010000052        27975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNV010000052 DAJTNV010000000
VERSION     DAJTNV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO199_002266"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OO199_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="OO199_002267"
     CDS             complement(998..2020)
                     /locus_tag="OO199_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5536283.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="OO199_002268"
     CDS             complement(2034..3536)
                     /locus_tag="OO199_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5536284.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OO199_002269"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OO199_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="OO199_002270"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="OO199_002270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5536285.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="OO199_002271"
     CDS             complement(5851..6192)
                     /locus_tag="OO199_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5536286.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OO199_002272"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OO199_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5536287.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OO199_002273"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OO199_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5536288.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="OO199_002274"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="OO199_002274"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5536289.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="OO199_002275"
     CDS             12871..14214
                     /locus_tag="OO199_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5536290.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="OO199_002276"
     CDS             complement(14276..14482)
                     /locus_tag="OO199_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5536291.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="OO199_002277"
     CDS             14807..15361
                     /locus_tag="OO199_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5536292.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="OO199_002278"
     CDS             complement(15424..16362)
                     /locus_tag="OO199_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536293.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="OO199_002279"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="OO199_002279"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5536294.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="OO199_002280"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="OO199_002280"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5536295.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="OO199_002281"
     CDS             complement(19573..19953)
                     /locus_tag="OO199_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5536296.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="OO199_002282"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="OO199_002282"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5536297.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="OO199_002283"
     CDS             21011..21976
                     /locus_tag="OO199_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5536298.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="OO199_002284"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="OO199_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5536299.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="OO199_002285"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="OO199_002285"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5536300.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="OO199_002286"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="OO199_002286"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5536301.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="OO199_002287"
     CDS             25350..25895
                     /locus_tag="OO199_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5536302.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="OO199_002288"
     CDS             complement(25879..26526)
                     /locus_tag="OO199_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5536303.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="OO199_002289"
     CDS             26738..27529
                     /locus_tag="OO199_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5536304.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>27975
                     /locus_tag="OO199_002290"
     CDS             27539..>27975
                     /locus_tag="OO199_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5536305.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIAL"
BASE COUNT         6881 a         7452 c         6884 g         6758 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctgga
//
LOCUS       DAJTNV010000053        27700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNV010000053 DAJTNV010000000
VERSION     DAJTNV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            49..156
                     /locus_tag="OO199_002291"
     CDS             49..156
                     /locus_tag="OO199_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5536306.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(243..1922)
                     /gene="bcsG"
                     /locus_tag="OO199_002292"
     CDS             complement(243..1922)
                     /gene="bcsG"
                     /locus_tag="OO199_002292"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5536307.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1919..2110)
                     /gene="bcsF"
                     /locus_tag="OO199_002293"
     CDS             complement(1919..2110)
                     /gene="bcsF"
                     /locus_tag="OO199_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5536308.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2107..3678)
                     /gene="bcsE"
                     /locus_tag="OO199_002294"
     CDS             complement(2107..3678)
                     /gene="bcsE"
                     /locus_tag="OO199_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5536309.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3951..4139
                     /locus_tag="OO199_002295"
     CDS             3951..4139
                     /locus_tag="OO199_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5536310.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4151..4903
                     /gene="bcsQ"
                     /locus_tag="OO199_002296"
     CDS             4151..4903
                     /gene="bcsQ"
                     /locus_tag="OO199_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5536311.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4900..5259
                     /locus_tag="OO199_002297"
     CDS             4900..5259
                     /locus_tag="OO199_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5536312.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5256..7517
                     /gene="bcsA"
                     /locus_tag="OO199_002298"
                     /pseudo
     CDS             5256..7517
                     /gene="bcsA"
                     /locus_tag="OO199_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7528..9867
                     /gene="bcsB"
                     /locus_tag="OO199_002299"
     CDS             7528..9867
                     /gene="bcsB"
                     /locus_tag="OO199_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5536313.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9874..10980
                     /gene="bcsZ"
                     /locus_tag="OO199_002300"
     CDS             9874..10980
                     /gene="bcsZ"
                     /locus_tag="OO199_002300"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5536314.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11039..11215
                     /locus_tag="OO199_002301"
                     /pseudo
     CDS             11039..11215
                     /locus_tag="OO199_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11543..13291
                     /gene="bcsC"
                     /locus_tag="OO199_002302"
                     /pseudo
     CDS             11543..13291
                     /gene="bcsC"
                     /locus_tag="OO199_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13373..15361
                     /gene="hmsP"
                     /locus_tag="OO199_002303"
     CDS             13373..15361
                     /gene="hmsP"
                     /locus_tag="OO199_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5536315.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15546..16832
                     /gene="dctA"
                     /locus_tag="OO199_002304"
     CDS             15546..16832
                     /gene="dctA"
                     /locus_tag="OO199_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5536316.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17053..18549
                     /locus_tag="OO199_002305"
     CDS             17053..18549
                     /locus_tag="OO199_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5536317.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18645..19574)
                     /gene="kdgK"
                     /locus_tag="OO199_002306"
     CDS             complement(18645..19574)
                     /gene="kdgK"
                     /locus_tag="OO199_002306"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5536318.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19806..20573
                     /gene="pdeH"
                     /locus_tag="OO199_002307"
     CDS             19806..20573
                     /gene="pdeH"
                     /locus_tag="OO199_002307"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5536319.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20643..22703
                     /locus_tag="OO199_002308"
     CDS             20643..22703
                     /locus_tag="OO199_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5536320.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22885..24207)
                     /locus_tag="OO199_002309"
     CDS             complement(22885..24207)
                     /locus_tag="OO199_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5536321.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24618..25631)
                     /gene="yhjD"
                     /locus_tag="OO199_002310"
     CDS             complement(24618..25631)
                     /gene="yhjD"
                     /locus_tag="OO199_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5536322.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25680..26579)
                     /locus_tag="OO199_002311"
     CDS             complement(25680..26579)
                     /locus_tag="OO199_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5536323.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            27099..>27700
                     /locus_tag="OO199_002312"
     CDS             27099..>27700
                     /locus_tag="OO199_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5536324.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6600 a         6986 c         7591 g         6523 t
ORIGIN      
        1 aactcaagaa tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca
       61 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      121 agtatgatcg tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      181 cgtaatggca atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt
      241 gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcttgg
      301 tattgaataa ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg
      361 gtgagttttt tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc
      421 accagatcgg agatagccag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga
      481 tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta
      541 gggatatcac gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc
      601 ggcaccacga ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc
      661 agttcatcaa agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga
      721 taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta
      781 tctttttcgg taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa
      841 ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg
      901 ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc
      961 aggtgctggg taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc
     1021 ggttgataca gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc
     1081 gccgggccac tgtaggaggt ggcggagtta aagttcttga aatcaatatc gaaatgcgac
     1141 cacagtggat gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg
     1201 ttaatcacca gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat
     1261 ttacgtttcg cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt
     1321 ggcggtgcag tttgtgcggg catatcaccc actaccggtg cgccacccgt cgccgcaacg
     1381 gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac
     1441 aaggagaaac ttggtcccgc caaggtaagt acgttcagcc atagcagtat ggcaaccaca
     1501 aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg
     1561 gccccaatca tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg
     1621 aaccccgcca cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccagatgtca
     1681 tgccagaaca aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg
     1741 taacgcggaa tgggcatcag cagaaacgcg gcaaacacca aattgaggag cgggtggaag
     1801 ttaagatatc ccgcccacaa caggccaaac ttaaccagaa aatagaagtt ccagccggaa
     1861 aggccgcgcc agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc
     1921 attttttggt tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac
     1981 gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca
     2041 gatagcccag cgggaaaaat atcagtgcgc aaacgataat aatttcaatg atatcgctga
     2101 tggtcatcat gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg
     2161 tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg
     2221 cagcggcatt ccccattgtt ctggggcaag caggcgcatc tgcaccagct cggcactgat
     2281 ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa
     2341 aatatgattc aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag
     2401 ccgattaccg ccaatggtca tgatatcgcc ggtacggtta gggcgacata gcgtcagggc
     2461 ttgctcaacg cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc
     2521 aggcaagaga gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg
     2581 gaaaccacgc agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggtac
     2641 atagcgacta aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg
     2701 cgcattccac ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc
     2761 gcgcaggcta gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg
     2821 cagggtatga atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac
     2881 caccgtcgcc gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag
     2941 ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg
     3001 tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc
     3061 attttgttgc tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa
     3121 ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga
     3181 acggtattcc tcaagcaata atgaaaattg tttatcgtta ttatttccgg gattaattac
     3241 caaaagcgaa caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag
     3301 ccgctcggca ggaatatttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt
     3361 atggggatca atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt
     3421 tagcattgaa aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt
     3481 cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat
     3541 tgtttgattc gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc
     3601 gcctgctggc atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg
     3661 gtccacaatg tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa
     3721 gaaataaacc taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa
     3781 ggtcacaaga cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa
     3841 agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg
     3901 gttagaattg tctcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca
     3961 atgaaccaga tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg
     4021 cgttaaagca ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac
     4081 agttggccgc ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg
     4141 ggattggtga atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac
     4201 catcaccgcc gcattagcct ggtcattaca aatgttggga gaaaatgtcc tggtggtcga
     4261 tgcctgcccg gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg
     4321 ctgggccaga gcgatgctgg atggccagga ctggcgtgac gctgggttgt gctacacctc
     4381 gcagctcgat ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca
     4441 ctggcaaacc cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg
     4501 ttaccagtgg attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct
     4561 gagtttgtgc gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact
     4621 gcatcagcaa gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag
     4681 tcaggttcag gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat
     4741 gctcattcat cgtgatgaag cgatggctga atgcctggcg gctaagcaac cagtaggtga
     4801 atatcgcagt gatgcgctgg cggctgaaga gatactgacg ctggcgaact ggtgcctgtt
     4861 gaactactcc gggctgaaaa cgccagtcgg gagtgcatca tgagtatcct gacccggtgg
     4921 ttgcttatcc cgccggtcaa cgcgcggctt atcgggcgtt atcgcgatta tcgtcgtcac
     4981 ggtgcgtcgg ctttcagcgc gacgctcggc tgtttctgga tgatcctggc ctggattttt
     5041 attccgctgg agcacccgcg ctggcagcgt attcgcgcag aacataaaaa cctgtatacg
     5101 catatcaacg cctcgcgtcc gcgtccgctg gacccggtcc gttatctcat tcaaacatgc
     5161 tggttattga tcggtgcatc gcgcaaagaa acgccgaaac cgcgcagcgg gcattttcag
     5221 gtctgcaaaa tattcgtgga cgttaccatc aatggatgaa cgagctgcct gagcgcgtta
     5281 gccataaaac acagcatctg gatgaaaaaa aagagctcgg tcatttgagt gccggggcgc
     5341 ggcggttgat cctcggtatc atcgtcacct tctcgctgat tctggcgtta atctgcgtta
     5401 ctcagccgtt taacccgctg gcgcagttta tcttcctgat gctgctgtgg ggggtagcgc
     5461 tgatcgtacg gcggatgccg gggcgcttct cggcgctaat gttgattgtg ctgtcgctga
     5521 ccgtttcttg ccgttatatc tggtggcgtt acacctctac gctgaactgg gacgatccgg
     5581 tcagcctggt gtgcgggctt attctgctct tcgctgaaac gtacgcgtgg attgtgctgg
     5641 tgctcggcta cttccaggta gtatggccgc tgaatcgtca gccggtgcca ttgccgaagg
     5701 atatgtcgct gtggccgtca gtggatatct ttgtcccgac ttacaacgaa gatctcaatg
     5761 tggtgaaaaa taccatttac gcctcgctgg gtatcgactg gccgaaagac aaactgaaca
     5821 tctggatcct cgatgacggc ggcagggaag agtttcgcca gtttgcgcaa aacgtggggg
     5881 tgaaatatat cgcccgcacc actcatgaac atgcgaaagc gggcaacatc aacaatgcgc
     5941 tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat
     6001 cgttcctgca aatgaccatg ggctggttcc tgaaagagaa acagctggcg atgatgcaga
     6061 ctccacacca cttcttctcg ccggacccgt ttgaacgcaa cctgggacgt tttcgtaaaa
     6121 cgccgaacga aggcacgctg ttctatggtc tggtgcagga tggcaacgat atgtgggacg
     6181 ccactttctt ctgcggttcc tgtgcggtga ttcgtcgtaa gccgctggat gaaattggcg
     6241 gcattgctgt cgaaaccgtg actgaagatg cgcatacttc tctgcgtttg caccgtcgtg
     6301 gctatacctc cgcgtatatg cgtattccgc aggcggcggg gctggcgacc gaaagtctgt
     6361 cggcgcatat cggtcagcgt attcgctggg cgcgcgggat ggtataaatc ttccgtctcg
     6421 ataacccgct caccggtaaa ggactgaagt ttgctcagcg gctgtgctac gtcaacgcca
     6481 tgttccactt cttgtcgggc attccacggc tgatcttcct gactgcgccg ctggcgttcc
     6541 tgctgcttca tgcctacatc atctatgcgc cagcgttgat gatcgcccta ttcgtgctgc
     6601 cgcatatgat ccatgccagc ctgaccaact cgaagatcca gggcaaatat cgccactctt
     6661 tctggagtga aatctacgaa acggtgctgg cgtggtatat cgcaccaccg acgctggtgg
     6721 cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc caaaggtgga ctggtggaag
     6781 aagagtacgt cgactgggtg atctcgcggc cctacatctt ccttgttcta ctcaacctgg
     6841 tgggcgttgc ggtaggcatc tggcgctact tctatggccc gccaaccgag atgctcaccg
     6901 tggtcgtcag tatggtgtgg gtgttctaca acctgattgt tcttggcggc gcagttgcgg
     6961 tatcggtaga aagcaaacag gtacgccgat cgaaccgcgt ggagatgacg atgcccgcgg
     7021 caattgcccg cgaagatggt cacctcttct cgtgtaccgt tcaggatttc tccgacggtg
     7081 gtttggggat caagatcaac ggtcaggcgc agattctgga agggcagaaa gtgaatctgt
     7141 tgcttaaacg cggtcagcag gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta
     7201 atgaagttgg gctgaaatta atgccgctca ccacccagca acatatcgat tttgtgcagt
     7261 gtacgtttgc ccgtgcggat acatgggcgc tctggcagga cagctacccg gaagataagc
     7321 cgctggaaag tctgctggat attctgaagc tcggcttccg tggctaccgc catctggcgg
     7381 agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct gacttctctg gtttcctggg
     7441 ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac ggcacaacca tcggatcagg
     7501 ctttggctca acaatgatga taacgcgatg aaaagaaaac tattctggat ttgtgcagtg
     7561 gctatgggga tgagtgcgtt cccctctttc atgacgcagg cgacgccagc aacgcaacca
     7621 ctgatcaatg ctgagccagc tgtagccgcc cagacggaac aaaatccgca ggtggggcaa
     7681 gtgatgtcag gcgagcaggg agctgatgcc cccatcgtgg cgcagaacgg tccttcgcgt
     7741 gatgtgaagc tgacctttgc gcaaatcgcg ccgccgccgg gcagcatggt gctacgtggt
     7801 attaacccga acggcagcat tgagtttggt atgcgcagcg atgaagtggt gacgaaggcg
     7861 atgctcaact tcgaatacac gccatcgcca tcgttactgc ctgtccagtc gcagttaaag
     7921 gtttatctca atgatgagct gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa
     7981 aaaacgctgg cgcaaatgcc cattaatcca ctgtttatta ccgacttcaa ccgtgtgcgg
     8041 ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg
     8101 ctggatgttg ggcggagcag tggactggat ctgacctatc agaccctgaa tgtgaagaat
     8161 gacctgtcac acttcccggt gccattcttt gacccgcgcg ataaccgtac taacaccttg
     8221 ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac aagcatctgc cattgtcgcc
     8281 tcgtggtttg gttcgcgttc tggctggcgt gggcagaact tcccggtgct ctataaccaa
     8341 ttgccggatc gcaatgccat tgtctttgca accaacgaca aacggccgga cttcctgcgc
     8401 gatcatccgg cggtaaaagc cccggtgatt gagatgatta accatccgca gaatccttac
     8461 gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt
     8521 atcgctcagg gtaacattct gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg
     8581 ctgctaccgc gtaagccgta cgatgcgccg aactgggtac gtaccgatcg tccggtcaca
     8641 tttggcgaac tgaaaaccta tgaagaacag ttacaatcca gcggccttga gccagcagcg
     8701 attaacgttt cgctaaacct gccgccggat ctctacctga tgcgcagtac cggcattgat
     8761 atggatatta attaccgcta caccatgccg ccggtgaaag acagttcgcg gatggatatc
     8821 agcctgaata accagttcct gcaatccttc aacctgagca gcaaacagga ggcgaaccgt
     8881 ctgctgctgc gtattccggt attacaaggt ttgctggatg gcaaaacaga tgtctctatt
     8941 ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg actttgagta tatgaacccg
     9001 atgccaggcg gttcggtaga taactgtatt accttccagc cggtgcagaa tcatgtggtg
     9061 attggtgacg attccaccat cgacttctcg aagtattacc acttcatccc gatgccggat
     9121 ctacgcgcct ttgctaacgc gggcttccca ttcagccgga tggcggatct gtcgcaaacc
     9181 atcaccgtga tgccgaaagc gcctaacgaa gcacagatgg aaacgttgct gaatactgtt
     9241 ggttttatcg gcgcacagac gggcttcccg gcgattaatc tgacggtgac cgatgatggc
     9301 agcaccattc agggcaaaga tgccgacatc atgatcatcg gtggtatccc ggacaaactg
     9361 aaagacgata agcagatcga cctattggtg caggcgaccg aaagctgggt gaaaacaccg
     9421 atgcgccaga ccccgttccc cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc
     9481 cagtcaactc tgacctcttc cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat
     9541 aacgaccagc gcagcgtgat tgcgctgttg gcagatagcc cacgcggtta tgaaatgctt
     9601 aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc
     9661 cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt attacgtagg tcatctgccg
     9721 tggttcgagc gcttgtggta tgcgctggca aaccatccga ttctgctggc ggtgctggcg
     9781 actatcagtg tgatattgct ggcatgggta ctgtggcgtc tgctgcgaat tattagtagt
     9841 cgtcgtctta acccggataa cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg
     9901 acgatgctgt tgttggctgc ctttagtgtt caggcagcct gtacctggcc tgcctgggag
     9961 cagtttaaaa aggattacat cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc
    10021 aaaatcacca cctccgaagg gcaaagttac ggtatgttct ttgccctggc ggctaacgac
    10081 cgtgcagctt tcgataatat tctcgactgg acgcagaaca atctcgctca gggttcttta
    10141 aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga acagtaagtg ggaagtgctg
    10201 gacagcaatt cggcctccga tggtgatgtc tggatggcct ggtcgttgct ggaggcgggg
    10261 cgtttgtgga aagagcagcg ttataccgac atcggcagcg cgttgctaaa acgtatcgcg
    10321 cgggaggaag tggtgacggt gcctgggctg ggttccatgt tgttaccggg caaagtgggt
    10381 tttgctgagg ataacagctg gcgttttaac cccagctaca tgccgccgac gctggcgcag
    10441 tatttcaccc gctttggcgc gccgtggacc acgctgcgcg aaaccaatca acatttattg
    10501 ctggaaaccg ccccgaaagg cttttcgcca gactgggtgc gctatgagaa agacaaaggc
    10561 tggcagctaa aagccgaaaa aacattgatc agcagctacg acgctatccg cgtttacatg
    10621 tgggtaggca tgatgcctga cagcgatccg caaaaagcgc ggatgctcaa ccggtttaaa
    10681 ccgatggcga cattcactga gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg
    10741 gggaaagcgc agggtaaagg accggtcggt ttttctgccg ccatgctgcc ttttttacaa
    10801 aaccgcgatg cgcaggcagt tcagcgccag cgcgtggccg ataactttcc cggcagcgat
    10861 acctattaca actatgtgct gaccctgttt ggacaaggct gggatcaaca ccgtttccgc
    10921 ttctcgacaa aaggtgagtt attacctgac tggggccagg aatgcgcaaa ttcacactaa
    10981 acatattcac gctttccctc ggtctggccg tcatgccgat ggtcgaggca gcaccaacca
    11041 tcagcagacc ctgcggatgg acagcggcaa cactaatgcc gtgcgcgggc tggcgaatat
    11101 ttaccgccag cagtcgccag aaaaagctga agcgtttatc gcctcgctct ctgccagcca
    11161 gcggcgcagc attgatgata tcgaacgcag cctgcaaaac gaccgtctgg cacagcaggc
    11221 agaggtactg gaaaaccagg gcaaatgggc gcaggcggca gcacttcagc ggcaacgact
    11281 ggcgctgggc cccggcagcg tatggattac ttaccgactt tcgcaggatc tctggcaggc
    11341 cggacaacgc agccaggccg atacgttaat gcgcaatctg gcgcagcaga agccgaacaa
    11401 cccggagcag gtttacgctt acgggctgta cctctcaggt cacaaccagg acagagcggc
    11461 gctggcgcat atcaacagcc tgccgcgcgc gcagtggaac agcaatattc aggagctggt
    11521 taatcgactg caaagcgatc aggtgctgga aaccgctaat cgcctgcgag aaagcggcaa
    11581 agaggctgaa gcggaagcga tgctgcgtca gcaaccacct tccacgcgta ttgacctcac
    11641 gctggctgac tgggcgtaac aacgacgtga ttacaccgcc gcccgcactg catatcagaa
    11701 tgtcctgacg cgggagccaa ctaacgccga tgccattctt ggtctgacgg aagtggatat
    11761 tgctgccggt gacaaagcgg cggcacgtag ccagctggcg aaactgcctg ccaccgataa
    11821 cacctcgctg aacactcagc ggcgcgtggc gctggcacag gcgcagcttg gagataccgc
    11881 agcggcgcag cagacgttta ataagttgat cccgcaggca aaatctcagc caccgtcgat
    11941 ggaaagcgcg atggtactgc gcgacggtgc gaagtttgaa gcgcaggcgg gcgatccaaa
    12001 gcaggcgctg gaaacctaca aagacgccat ggtcgcatcc ggtgtgacta cgacgcgtcc
    12061 gcaggataac gacaccttta cccgactgac ccgtaacgac gagaaagatg actggctgaa
    12121 acgcggcgtg cgcagcgatg cggcggacct ctatcgccag caggatctta acgtcaccct
    12181 tgagcacgat tactggggtt cgagcggcac cggtggttac tccgatctga aagcgcacac
    12241 taccatgttg caggtggatg cgccgtattc tgacgggcgg atgttctttc gcagtgattt
    12301 cgtcaatatg aacgtcggca gtttctccac taatgccgat ggcaaatggg atgacaactg
    12361 gggcacctgt acattacagg actgtagcgg caaccgcagc cagtcggatt ccggtgccag
    12421 cgtggcggtc ggctggcgaa atgacgtctg gagctgggat atcggtacca cgccgatggg
    12481 cttcaacgtg gtggatgtgg tcggcggcat cagttacagc gatgatatcg ggccgctggg
    12541 ttacaccgtt aacgcccacc gtcggcccat ctccagttct ttgctggcct ttggtgggca
    12601 aaaagactcc ccgagcaata ccgggaaaaa atggggtggc gtacgtgccg acggtgtggg
    12661 gctaagtctg agctacgata aaggtgaagt aaacggcgtc tgggcatcgc ttagtggcga
    12721 ccagttaacc ggtaaaaatg tcgaagataa ctggcgcgtg cgctggatga cgggctatta
    12781 ctataaggtc attaaccaga acaatcgccg cgtcacaatc ggcctgaaca acatgatctg
    12841 gcattacgac aaagatctga gtggctactc actcggtcag ggcggttact acagtccgca
    12901 ggaatacctg tcgtttgcca taccggtgat gtggcgggag cgcacggaaa actggtcgtg
    12961 ggagctgggg gcgtctggct cgtggtcgca ttcacgcacc aaaaccatgc cgcgttatcc
    13021 gctgatgaat ctgatcccga ccgactggca ggaagaagct gcgcggcaat ccaacgatgg
    13081 cggcagcagt cagggcttcg gctacacggc gcgggcatta cttgaacgac gtgttacttc
    13141 caactggttt gttggtacgg caattgatat ccagcaggcg aaagattacg cacccagcca
    13201 tttcctgctc tacgtacgtt attccgccgc cggatggcag ggtgacatgg atttaccgcc
    13261 gcagccgctg ataccttacg ccgactggta agttttcaga tagcgcctct cttaatgccg
    13321 ctgcgatcgg gtatactcgg gcggcaatct gggatttccg gggggagaca atttgcgcgt
    13381 aagtcgctcg ttaacaatca agcagatggc aatggtggca gccgttgtcc tggtgttcgt
    13441 ttttattttt tgcaccgttt tgctgttcca tctggtccag cagaatcgct ataacacggc
    13501 tacgcaactg gaaagcattg ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa
    13561 aggcgatatt cccgaagcgg aagctattct tgccagcatt aaaccggcag gcgtggtcag
    13621 ccgtgccgat gtagtgctgc ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga
    13681 gcgtccggtg ccggtaatgg ttactcgcct gtttgagcta ccggttcaaa tctcgctggg
    13741 cgtctactcg ctcgaacgtc cggcaaatcc gcagccaata gcctatctgg tgctacaggc
    13801 ggattccttc cgtatgtata agttcgtgat gagcactctc tcaacgttag tgaccattta
    13861 cttacttttg tcgcttatcc tgaccgtcgc catcagctgg tgcattaacc gcctgatttt
    13921 gcatccgtta cgcaatattg ctcgcgaact taacgccatc ccagcccagg agcttgttgg
    13981 tcaccaactg gcattaccgc gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag
    14041 ttacaacctc aaccagcaat tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat
    14101 gcgcttcccg gtgtcggatt tgccgaacaa agccttgctg atggagatgc tggagcaggt
    14161 tgtcgcgcgt aaacaaacca ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac
    14221 tgcgggcgtg ctgaaagagg cgcaacgaga aattctgctg ctgacgctgg tggaaaaact
    14281 caaatcggta ctgtcgccac gtatgatcct cgcgcagatt agcggttatg actttgctgt
    14341 cattgccaac ggtgtacagg aaccgtggca cgcaatcact ttgggtcagc aagtactcac
    14401 tatcatgagc gagcgcctgc cgattgaacg tattcaactc cgtccgcact gtagcattgg
    14461 cgtggcgatg ttctacggcg atctcaccgc cgaacagctt tacagtcgcg ctatttctgc
    14521 ggcatttacc gctcgccata aaggcaagaa tcagattcag ttctttgatc cgcagcagat
    14581 ggaagcagcc cagaagcggt tgacggaaga gagcgatatc cttaatgcac tggaaaatca
    14641 tcagtttgca atttggttac agccacaggt cgagatgacc agcggtaaac tggtcagtgc
    14701 ggaagtgtta ctgcgtatcc agcaaccgga tggcagttgg gacctgccgg atggcttaat
    14761 cgatcgcatt gagtgctgtg ggctgatggt taccgtcggt cactgggtgc tggaagagtc
    14821 ctgtcgattg cttgcagcct ggcaagagcg cggcattatg ctgcccttgt cggtaaactt
    14881 ctctgcgctg caactgatgc acccgaatat ggtggcggat atgctggaac tgttaacccg
    14941 ctatcgcatt cagccgggaa cactgattct ggaagtgaca gaaagccgac gtattgacga
    15001 ccctcatgct gcggtggcaa tcctccgtcc gctgcgcaat gccggagttc gggtggcgct
    15061 ggatgatttc ggcatgggct acgcagggct gcgtcagctg cagcatatga aatcgttgcc
    15121 aatcgacgta ctgaaaatcg acaaaatgtt tgttgaaggc ttgccggaag atagcagcat
    15181 gattgctgca attatcatgc tggcgcagag cctgaactta caaatgattg ccgaaggcgt
    15241 ggagactgaa gcacaacgag actggctggc aaaagcgggc gttggtattg cccagggctt
    15301 cctttttgct cgcccactcc ctattgaaat cttcgaagag agttacctgg aagaaaagta
    15361 gctaccccaa actgattaca aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac
    15421 tttttaacct ttttgtttca attatgatcc aggtacattt ctgtgatgtt gtctgggtgt
    15481 tattttaagg ccgcaggtac cccccataac cttacaagac ctgtggtttt actaaaggac
    15541 accctatgaa aacctctctg tttaaaagcc tttactttca ggtcctgaca gcgatagcca
    15601 ttggtattct ccttggccat ttctatcctg aaataggcga gcaaatgaaa ccgcttggcg
    15661 acggcttcgt taagctcatt aagatgatca tcgctcctgt catcttttgt accgtcgtaa
    15721 cgggcattgc gggcatggaa agcatgaagg cggtcggtcg taccggcgca gtcgcactgc
    15781 tttactttga aattgtcagt accatcgcgc tgattattgg tcttatcatc gttaacgtcg
    15841 tgcagcctgg tgccggaatg aacgtcgatc cggcaacgct tgatgcgaaa gcggtagcgg
    15901 tttacgccga tcaggcgaaa gaccagggca ttgtcgcctt cattatggat gtcatcccgg
    15961 cgagcgtcat tggcgcattt gccagcggta acattctgca ggtgctgctg tttgccgtac
    16021 tgtttggttt tgcgctccac cgtctgggca gcaaaggcca actgattttt aacgtgatcg
    16081 aaagtttctc gcaggtcatc ttcggcatca tcaatatgat catgcgtctg gcacctattg
    16141 gtgcgttcgg ggcaatggcg tttaccatcg gtaaatacgg cgtcggcaca ctggtgcaac
    16201 tggggcagct gattatctgt ttctacatta cctgtatcct gtttgtggtg ctggtattgg
    16261 gttcaatcgc taaagcgact ggtttcagta tcttcaaatt tatccgctac atccgtgaag
    16321 aactgctgat tgtactgggg acttcatctt ccgagtcggc gctgccgcgt atgctcgaca
    16381 agatggagaa actcggctgc cgtaaatcgg tggtggggct ggttatcccg acaggctact
    16441 cgtttaacct tgatggcaca tcgatatacc tgacaatggc ggcggtgttt atcgcccagg
    16501 ccactaacag tcagatggat atcgtccacc aaatcacgct gttaatcgtg ttgctgcttt
    16561 cttctaaagg ggcggcaggg gtaacgggta gtggctttat cgtgctggcg gcgacgctct
    16621 ctgcggtggg ccatttgccg gtagcgggtc tggcgctgat cctcggtatc gaccgcttta
    16681 tgtcagaagc tcgtgcgcta actaacctgg tcggtaacgg cgtagcgacc attgtcgttg
    16741 ctaagtgggt gaaagaactg gaccacaaaa aactggacga tgtgctgaat aatcgtgcgc
    16801 cggatggcaa aacgcacgaa ttatcctctt aatctcacca cttatgcccg cactcccttc
    16861 cccgagtgcg ggtgtttgcg cataattgac ccctgtgcct gaacttacca cctgggtgag
    16921 ttcaggttta tttcacttct tccgtgacat tttcatgttc ttgcggtcta acacgaagtg
    16981 tttttaacgt catttagact gaccgacaac gggcagtttt tttattacca ggatagttga
    17041 tcaggggttc acatgcaggg cataaaaatt cgacttttag cgggcggttt gctgatgatg
    17101 gccactgctg gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg
    17161 acgctttcca acggtttaca gtggcaagtg ctgaccaccc cccagcgtcc cagcgatcgt
    17221 gttgaaattc gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt
    17281 tacagtcacg ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcacag
    17341 gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc
    17401 tcttatgaca ccacgctgtt taatctgagt ttgcccaata accgtaacga tttgctgaaa
    17461 gaagcgctct cttatctggc aaatgccact ggcaaattga ccatcacacc agaaaccatc
    17521 aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc
    17581 tggtggcgct atcgtctgaa agggtcaacc ttgttaggtc acgatcctgc cgatccgctg
    17641 aaacaacccg ttgaagcgga aaaaattaaa gatttctatc agaaatggta caccccggat
    17701 gcaatgacgc tactggtggt gggaaacgtg gatgcgcgct cggttgtcga ccaaatcaac
    17761 aaaacgtttg gcgaactgaa aggcaaacgt gaaacgccag ctccggtgcc gacgctttct
    17821 ccgctgcgtg cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatct
    17881 atcatgtggg atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg
    17941 cgtgcggacc tggcccgcga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt
    18001 gacagcaaag acatcggtct tggatttgac tgccgtgtgc tgtatctgcg tgcgcagtgt
    18061 gccatcaaca tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt
    18121 gaactggcga aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg
    18181 caaaagaaac tggagctgca gaaactgttt gccgcctatg cacgggctga taccgatatt
    18241 ctgatgggcc agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcag
    18301 tatcagaaac tgcggcagga tttccttaac agcctgactg tggagatgtt aaatcaggat
    18361 ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg
    18421 gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atctaccgcc
    18481 gctgcgacca cctctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct
    18541 gcacagtaat gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg
    18601 gatagagggg taacacttta tccagccttt tgcatatgct gcgtttacgc tggcatcgcc
    18661 tcacgcggga taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt
    18721 ttcgccgcgt tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcgctgaaa
    18781 gagtcgccag ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgccggaaca
    18841 tcaactaacg cttcaccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc
    18901 acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc
    18961 cacagcgcgt cttcgtcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgc
    19021 tggtacacct gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg
    19081 aaaatcactt ttccgccttt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg
    19141 ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc
    19201 agctcttcgc aaatcgccgc agactgctcg ctcgccagcc agaatttggc ggcggcttcg
    19261 ttacgccagt agtagaacgt acgttcgccg gtgctgtcgg tttcaatgta gtaaaggccc
    19321 ggaagacggt tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gtgccaggcg
    19381 tccagcatct gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat
    19441 gccgcaggat cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa
    19501 ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca
    19561 atctttttgg acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg
    19621 gtggtttgct ggtcaaccat attaaaacag tgttccatta tttttttgag ccagaacgta
    19681 ttcctgaaag attccgttgt ggagtgagga aatttacccc ggccaggcgg taaagttctg
    19741 cccttacgcg ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat
    19801 ctttgatgat aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct
    19861 tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc
    19921 aaacatgcgg gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc
    19981 cttcgcaacg cctgccgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg
    20041 aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc cgacttcttt gtagagcacg
    20101 gcctgctggc atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa
    20161 aaatcctgcg ccagattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatcc
    20221 gtctgccgaa agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg
    20281 attttggtac cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca
    20341 aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc ggaaggccgc acactctttt
    20401 ctcagctttt acatctaatg aatcgctatt gtcgcggggt gattgtcgag ggcgtagaaa
    20461 caccggaaga gtggcgtgat gttcagaact cgcccgcatt cgccgcacaa ggctggtttc
    20521 tttcacgccc ggcaccgatg gaaacgctga atacggcggt tctggcgcta taagctgcct
    20581 cattttccgc cgggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa
    20641 cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg
    20701 tcgtggcgat cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc
    20761 agaaagtctc tgcggagttg aatcgtccgt tcgctatccg tggcaatctg ggcgtggtgt
    20821 gggagcggca aaaacaagaa actggctggc gcagctgggt gccgtggcct catgtacatg
    20881 cagaagacat cattcttggt aatccaccgg atattcccga agtcacgatg gtgcatttgc
    20941 cgcgagtaga ggcaacgctg gcaccgctgg cgctgctgac caaaacggtc tggctgccgt
    21001 ggatcaagct cgaaaagccc gacgcgcgcc tgattcgcct ctctgaaaag aacaataact
    21061 ggacgtttaa tcttgccaac gatgacaaca aagacgcgaa tgcaaagccg tcggcatggt
    21121 cgtttcggct ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa
    21181 gcaaagcgga tctggaaatt tttgttgatc cgttaggcaa gccgctgcca ttcagcgaag
    21241 ttactggatc gaaaggtaaa gcggataaag aaaaggtggg cgattacatt tttggcctga
    21301 aggcgcaggg acgttataac ggtgaaccgc tcacgggtac gggaaaaata ggcggtatgc
    21361 tggcgctgcg tggcgaaggg acgccgtttc cggtacaggc tgatttccgt tcaggtaata
    21421 cccgtgttgc ttttgatggc gtcgtgaatg acccaatgaa gatgggcggt gtcgatttac
    21481 ggcttaaatt ttctggcgat tcactgggtg atctctatga actgacgggc gttctgctgc
    21541 ccgatacccc gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat
    21601 cgtcggtctt tgattatcgc ggttttaatg ggcgaattgg cgatagcgat atccacggtt
    21661 ctctggtcta caccaccgga aagccacgac caaaactgga aggtgatgtc gagtcgcggc
    21721 aattgcggct ggcggacctg ggaccgttga ttggcgttga ttccgggaaa ggggcagaaa
    21781 agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggcaaagtgc
    21841 tgccttatga ccgcttcgaa accgataaat gggacgttat ggatgccgat gttcgcttca
    21901 aagggcggcg cattgagcat ggcagtagcc tgccgattag cgatctttct actcatatca
    21961 tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggcggcagca
    22021 ttgcggcgaa tattcatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc
    22081 aggctcgtcg actgaaactg aaagaactga tgcccgatgt ggaactgatg cagaagacgc
    22141 tgggggaaat gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt
    22201 taggcaacag taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc
    22261 tgatggagat tgttgggctg aatgtcggca actacattgt cggtgcgata tttggtgacg
    22321 atgaggtgcg ggtgaactgc gcggcggcga atctgaatat tgccaacggc gtggcgcgcc
    22381 cgcagatttt tgctttcgat actgagaacg cgttgattaa tgttaccggc acggcaagtt
    22441 ttgcttcgga acagctggat ttgactattg atccggagag taaagggatt cggattatca
    22501 cactgcgttc gccgctgtat gtgcgtggga cgtttaaaaa tccgcaggct ggggtgaaag
    22561 ccggaccgct gattgcccgt ggtgctgttg ctgcggcact ggcaacgctg gtaacaccgg
    22621 cggcggcgtt actggcactg atctcacctt ccgaagggga ggctaatcag tgtcggacga
    22681 tattgtcgca gatgaagaag tgatgataat tgccggatgt ctgacatccg gcaattaaac
    22741 agatttagat ttttggcaaa gtgtgctttg ttcatgccgg atgcggcgtg aacgccttat
    22801 ccggcctaca aatccttgtg aattcaatat attgtatgga aattcagtcc tgataagcgt
    22861 agcgcatcag gcttttcaca cttattacaa cgactgatgt cgcgtctcat gggtcagcag
    22921 cagggcgatt aacgtcaagc cagccatcgc cgccagatat aaccccaccg cacctaaccc
    22981 gtagttagcc tgcaaccagg ctgcgatata cggcgcaacg gaagccccga gaatcgacga
    23041 tacgttgtaa gagaacgatg ctccggtgta acgcacttct gtcggaaaca gctctggtaa
    23101 cagcgcaccc atcggcccga aggtcagccc catcagactt aaccccagca gcaggaaggc
    23161 aaaaaccaga atcgggttgc cagaaccgag cagcgggtta aaggcgaaca gcgcgaacag
    23221 gatgatcagc gtggtgatga tcaccatgct tttacggcga ccaaaggcat cagccagtaa
    23281 tccggcgact ggcaccatca cgccaaaacc aataactgcc atcatcagca tccacaacac
    23341 ttcgttacgc ggcaggccaa gcccaactgg cgcggcggcg gtactgaagg tcatagagta
    23401 gaccgtcatg atgtaaaaca gcgtataggt tgccagcata atgaacgtac ccagtacggt
    23461 tacgcgaaca tgtttggtca gcagcgtacc cagcgggatc ttcacctgtt ttttcgcttt
    23521 agcgactttc tcaaacaccg gcgactcatg cagcgacacg cgaacataca ggccgataat
    23581 gaccagcacc gccgagaaga taaatggcac acgccagccc cagctcataa actgctcatc
    23641 ggtcagtagc caggaaagca gcaagaaagt gccgttggca aagaagaagc caatcggtgc
    23701 gcctagctgt gggaaggagc catacagtgc acgtttgcgc ggtggggcgt tttcagtcgc
    23761 cagcagcgcc gcaccgcccc attcaccgcc taagcccaga ccctgaccaa atcgagccag
    23821 cgccagcagc agtggggcga aaataccaat cgtggcatag cccggcagca gaccaatcac
    23881 cacggtcgaa atccccatcg ttagcaacga ggcgaccagc gtcgctttac gcccaacgcg
    23941 atcgccaaaa tgaccaaaaa cggcagagcc aatggggcgc gcgacgaagg cgatggcgaa
    24001 ggtggcgagc gactgtagcg ttgctgccgt tggatcgccc tgcgggaaga agatatgcgg
    24061 aaacacaata acggccgcag tggcgtaaat gtaaaagtcg aagaactcaa tggccgtgcc
    24121 aatgagagag gcgacaagga ctttattacg cgagttgatc ggcgtgtatt cttgttcgtg
    24181 gtcgagtgtt gtggctgttg cttgcataat cttttcttat tttttggcta acgaatagcc
    24241 atattacgca cagcaaaagt gatatttcaa tgtgtaacaa atcgcggaaa tggtcaaaaa
    24301 ccaggcaaaa aggggataat gtttaggcca gtgatttttc ttctatgaaa tgcttcacat
    24361 aattttataa atagtggata aaactggttt caggttaaat aaaagttacc aactggattt
    24421 atatgctggg gttatgagtt ggagtgaaat tgtttgctcc caaatagatg gctgaagagg
    24481 ctctggggaa gagtttcaag aggttatgtg cgcattatag ccggatgcga tgctggcgca
    24541 tcttatccgg cctacgggga gccaggccga ataaaatcgt atgccggata aggcgtttac
    24601 gccgcatccg gcctatttta aggctgcgtt ttccctggca ttcgcgggtc gtctttatat
    24661 tcggcggtgg caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa gtagaaaaat
    24721 gccatcagcc ccagcacgga accaaatgct gcgccagagg gggatttcat cagcgatggc
    24781 agggtgtagg tcatcacgat tttaatcact tcaaaaccaa tagcagccag aaatgttccg
    24841 cgaatcaggg cttttttgcg cggacggtgg cgcggcaacc gccagaagat ccagaagaaa
    24901 agcagatagt tggcgaagat ggaaatcgcc aggccaatca atcgccacgt cggtttcagc
    24961 cactcaatgc tgttcaggtg cagggcgcta ataatcattt gctgcgccga accggcaacc
    25021 gaggtgatcg aaagcgtcac aatcagcgca atcaacaaac caatcagtga aataaaatca
    25081 cgcagatatt ttacccagaa cttctcctga tcttgcggcg agcgttccca gacatcgcgc
    25141 gactgggcac gaatcgcttc acgcaggtta cccatccagt tgatgccgga ataaagtgcc
    25201 accgccaggc cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt gatggtgttt
    25261 ttcaacgtgg cggctagtgt cggatcgctg atgttttgca gaattttgtc gaagatatcc
    25321 tgtagcaaca tcggatggga ggccagcaca aagccccctg cggcaaacga caccatcaaa
    25381 atcgggatca tcgacaaaaa tgagaaatag gtgatagccg caccaaactg gttacccagc
    25441 cgatcgttaa agcgttctgt cgcgcgaata aggtgcgcaa taaccggttg gcgttggact
    25501 ttttcggcag tggtggtgac ggtttccagc gcctggctaa ctttgccgcc tttctcgcta
    25561 ttatccaact gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt gatctcgttt
    25621 tcctgcgtca tccgctcttt tgtccttctg tcgtggtcat tcttaaaaag tatagtcggt
    25681 cagtccaggt gctctttcat tacgccagcc agccactgca taaacaggtt tacacgccgg
    25741 gaaagctccc gacgctgcgg gtaaaccagc gaaagggaga gcggctcggc acggtagcct
    25801 ggcaacactt caataagccg cccggcacgc agggcttcgc gcacggcaat gcgcgggatc
    25861 tgaataatcc ccagaccggc aagaccggcg gcgagatagt tttcgctact gtttaccgtc
    25921 aacatgccgc cagacttaaa ccactggacg ccattaacgc tggcaacctc aaaacctaac
    25981 ggatgtacac ccaggtgcgg tgtataacgc actattgcgt gtgaagtcag atcatcgggg
    26041 ctttgcggat aaccaaagcg cgtcagatag tgcggactgg cacagttgac catggttagt
    26101 ttgccgaggg gacgggcgat aacgccatct tccggtaacg cgccagtgcg tatcacgcaa
    26161 tcaaaaccat catgaagaat atctaccgga cggttatggc tactcagttc cagctcaatt
    26221 cccggatgct gatagagaaa ttccgacaga cgcggcagta acaggttttt cgcgattcct
    26281 gacgggatgt cgatgcgtaa tttaccgctg atactggtgg catcctgttg aaacagaccg
    26341 tccagttcgc tgagattact caacacatct tttgcccgtt gataataggt catgccttct
    26401 ggcgtgagtt tgacccgtcg cgtagtgcgc tggagaagct gggtgccaag ttgatgttcc
    26461 agtgcctgta tctggcgcga aacacttccc tttggcaaag caaagaaatc cgccgcgcgg
    26521 gaaaaacttt ccagctccgc gactttgatg aacaactgca ttgcgtgaat tttatccatt
    26581 tctttgaccg attgttgttc acagcgaaac agtaacgcgc atatcgcatt gtttattgtt
    26641 ttttctaaca tctaatgccg ggtttcacaa tgttgtcatg acgaatgtgg cgatttctca
    26701 gcaggaaaag ggcggaatag ctttatgaga aaggtgaatc cccttaaaca gagcgtattt
    26761 ttcagtgatt ccctttaaag tgaatagagg acgtattggc tatttgtgct tatttttgtc
    26821 tcattgccat ttgttatttt ggcttgtagt cgcggaattt catttttaat tgcatgcgta
    26881 ctaaccacca atataaggaa atcctcaaaa ttgttttaaa tagtgccctc gacatgtaat
    26941 ttcaggcgtt tagctgtaac tatttatatc ggtaactatt ttctacgcgt catctctgta
    27001 gaatggccac cgccaaatgg cgaattctta ttttttctcg cgggtaatac ccttccacta
    27061 cttaagtctt tttaacgtat tcaggtggag attcagttat gcaaatagtc atgtttgaca
    27121 ggcagtcaat atttattcat ggaataaaaa tcagtttaca gcagcgtatt ccaggagtga
    27181 gtattcaggg ggtcagtcag gcagacgagt tatggcaaaa gctggaaagt taccctgaag
    27241 ccttagttat gctcgatggc gatcaggatg gtgagttttg ctactggttg ctgcaaaaaa
    27301 ccgtggtgca gtttcctgag gttaaggtgt taatcacggc gacggattgc aataaacggt
    27361 ggttacagga agttatccat tttaatgtgc tggccattgt gcctcgcgat tcaaccgtcg
    27421 agacatttgc gttggcggta aatagtgcgg cgatggggat gatgtttctg cctggagact
    27481 ggcggacgac gccggaaaag gacattaaag atctcaaatc gttaagcgcc cgccagcggg
    27541 agattttaac catgttagcg gcgggcgaat caaataagga gatcggcaga gcgctgaata
    27601 tcagtaccgg aacggtaaaa gcgcatctgg aatccctgta tcgccgtctg gaagtgaaaa
    27661 atcgcaccca ggcggcaatg atgttaaata tctcctcctg
//
LOCUS       DAJTNV010000054        27536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNV010000054 DAJTNV010000000
VERSION     DAJTNV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO199_002313"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO199_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5536325.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO199_002314"
     CDS             complement(1614..1937)
                     /locus_tag="OO199_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5536326.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO199_002315"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO199_002315"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5536327.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO199_002316"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO199_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5536328.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLSDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO199_002317"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO199_002317"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5536329.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO199_002318"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO199_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5536330.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO199_002319"
     CDS             7537..8574
                     /locus_tag="OO199_002319"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5536331.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO199_002320"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO199_002320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5536332.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO199_002321"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO199_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5536333.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO199_002322"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO199_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5536334.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO199_002323"
     CDS             10745..11626
                     /locus_tag="OO199_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5536335.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO199_002324"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO199_002324"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5536336.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO199_002325"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO199_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5536337.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO199_002326"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO199_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO199_002327"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO199_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5536338.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO199_002328"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO199_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5536339.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO199_002329"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO199_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5536340.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO199_002330"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO199_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5536341.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO199_002331"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO199_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5536342.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO199_002332"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO199_002332"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5536343.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO199_002333"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO199_002333"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5536344.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO199_002334"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO199_002334"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5536345.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO199_002335"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO199_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5536346.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO199_002336"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO199_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5536347.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO199_002337"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO199_002337"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5536348.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO199_002338"
     CDS             24893..25273
                     /locus_tag="OO199_002338"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5536349.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO199_002339"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO199_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5536350.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO199_002340"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO199_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5536351.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27536
                     /locus_tag="OO199_002341"
                     /pseudo
     CDS             26442..27536
                     /locus_tag="OO199_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6794 a         6854 c         7219 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggtcgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgctcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggcg gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaa
//
LOCUS       DAJTNV010000055        26563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNV010000055 DAJTNV010000000
VERSION     DAJTNV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            348..641
                     /locus_tag="OO199_002342"
     CDS             348..641
                     /locus_tag="OO199_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5536352.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO199_002343"
     CDS             713..1372
                     /locus_tag="OO199_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5536353.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO199_002344"
     CDS             1464..1910
                     /locus_tag="OO199_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5536354.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO199_002345"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO199_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO199_002346"
     CDS             3390..3809
                     /locus_tag="OO199_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5536355.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO199_002347"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO199_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5536356.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO199_002348"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO199_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5536357.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO199_002349"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO199_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5536358.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OO199_002350"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OO199_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5536359.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OO199_002351"
     CDS             complement(8332..9864)
                     /locus_tag="OO199_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5536360.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OO199_002352"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OO199_002352"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5536361.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OO199_002353"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OO199_002353"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5536362.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OO199_002354"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OO199_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OO199_002355"
     CDS             complement(14765..16501)
                     /locus_tag="OO199_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5536363.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO199_002356"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO199_002356"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5536364.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OO199_002357"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OO199_002357"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5536365.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OO199_002358"
     CDS             complement(18223..18957)
                     /locus_tag="OO199_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5536366.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OO199_002359"
     CDS             19158..19412
                     /locus_tag="OO199_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5536367.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OO199_002360"
     CDS             complement(19462..20922)
                     /locus_tag="OO199_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5536368.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OO199_002361"
     CDS             complement(20859..21416)
                     /locus_tag="OO199_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5536369.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OO199_002362"
     CDS             complement(21442..22296)
                     /locus_tag="OO199_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5536370.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OO199_002363"
     CDS             complement(22296..23105)
                     /locus_tag="OO199_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5536371.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OO199_002364"
     CDS             complement(23115..23873)
                     /locus_tag="OO199_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5536372.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OO199_002365"
     CDS             complement(23870..24850)
                     /locus_tag="OO199_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5536373.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OO199_002366"
     CDS             complement(24850..25872)
                     /locus_tag="OO199_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5536374.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6683 a         6473 c         6850 g         6557 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tcc
//
LOCUS       DAJTNV010000056        26074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNV010000056 DAJTNV010000000
VERSION     DAJTNV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO199_002367"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO199_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5536375.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO199_002368"
     CDS             complement(2138..2584)
                     /locus_tag="OO199_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5536376.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO199_002369"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO199_002369"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5536377.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO199_002370"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO199_002370"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5536378.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO199_002371"
     CDS             complement(5347..6009)
                     /locus_tag="OO199_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5536379.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO199_002372"
     CDS             6182..6595
                     /locus_tag="OO199_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5536380.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO199_002373"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO199_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5536381.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO199_002374"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO199_002374"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5536382.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO199_002375"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO199_002375"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5536383.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO199_002376"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO199_002376"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5536384.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO199_002377"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO199_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5536385.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO199_002378"
     tRNA            complement(9861..9948)
                     /locus_tag="OO199_002378"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO199_002379"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO199_002379"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5536386.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO199_002380"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO199_002380"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5536387.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO199_002381"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO199_002381"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5536388.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO199_002382"
     CDS             14005..14592
                     /locus_tag="OO199_002382"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5536389.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO199_002383"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO199_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5536390.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO199_002384"
     CDS             14984..15841
                     /locus_tag="OO199_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5536391.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO199_002385"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO199_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5536392.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO199_002386"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO199_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5536393.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO199_002387"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO199_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5536394.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO199_002388"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO199_002388"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5536395.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO199_002389"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO199_002389"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5536396.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO199_002390"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO199_002390"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5536397.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OO199_002391"
     CDS             complement(22898..24037)
                     /locus_tag="OO199_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5536398.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="OO199_002392"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="OO199_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="OO199_002393"
     CDS             complement(25322..26017)
                     /locus_tag="OO199_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5536399.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6216 a         6648 c         6905 g         6305 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcg
//
LOCUS       DAJTNV010000057        25356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNV010000057 DAJTNV010000000
VERSION     DAJTNV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..1328
                     /gene="yjeM"
                     /locus_tag="OO199_002394"
                     /pseudo
     CDS             1..1328
                     /gene="yjeM"
                     /locus_tag="OO199_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1479..4790)
                     /gene="mscM"
                     /locus_tag="OO199_002395"
     CDS             complement(1479..4790)
                     /gene="mscM"
                     /locus_tag="OO199_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5536400.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4812..5780)
                     /gene="psd"
                     /locus_tag="OO199_002396"
     CDS             complement(4812..5780)
                     /gene="psd"
                     /locus_tag="OO199_002396"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5536401.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5877..6929)
                     /gene="rsgA"
                     /locus_tag="OO199_002397"
     CDS             complement(5877..6929)
                     /gene="rsgA"
                     /locus_tag="OO199_002397"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5536402.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7024..7569
                     /gene="orn"
                     /locus_tag="OO199_002398"
     CDS             7024..7569
                     /gene="orn"
                     /locus_tag="OO199_002398"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5536403.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7780..7855
                     /locus_tag="OO199_002399"
     tRNA            7780..7855
                     /locus_tag="OO199_002399"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7813..7815,aa:Gly,seq:gcc)
     gene            7891..7966
                     /locus_tag="OO199_002400"
     tRNA            7891..7966
                     /locus_tag="OO199_002400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7924..7926,aa:Gly,seq:gcc)
     gene            8199..8252
                     /locus_tag="OO199_002401"
     CDS             8199..8252
                     /locus_tag="OO199_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536404.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8235..9374)
                     /gene="queG"
                     /locus_tag="OO199_002402"
     CDS             complement(8235..9374)
                     /gene="queG"
                     /locus_tag="OO199_002402"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5536405.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9373..10920
                     /gene="nnr"
                     /locus_tag="OO199_002403"
     CDS             9373..10920
                     /gene="nnr"
                     /locus_tag="OO199_002403"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5536406.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            10892..11353
                     /gene="tsaE"
                     /locus_tag="OO199_002404"
     CDS             10892..11353
                     /gene="tsaE"
                     /locus_tag="OO199_002404"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5536407.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11372..12709
                     /gene="amiB"
                     /locus_tag="OO199_002405"
     CDS             11372..12709
                     /gene="amiB"
                     /locus_tag="OO199_002405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5536408.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12719..14566
                     /gene="mutL"
                     /locus_tag="OO199_002406"
     CDS             12719..14566
                     /gene="mutL"
                     /locus_tag="OO199_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5536409.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14559..15509
                     /gene="miaA"
                     /locus_tag="OO199_002407"
     CDS             14559..15509
                     /gene="miaA"
                     /locus_tag="OO199_002407"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5536410.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15595..15903
                     /gene="hfq"
                     /locus_tag="OO199_002408"
     CDS             15595..15903
                     /gene="hfq"
                     /locus_tag="OO199_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5536411.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            15979..17259
                     /gene="hflX"
                     /locus_tag="OO199_002409"
     CDS             15979..17259
                     /gene="hflX"
                     /locus_tag="OO199_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5536412.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17345..18604
                     /gene="hflK"
                     /locus_tag="OO199_002410"
     CDS             17345..18604
                     /gene="hflK"
                     /locus_tag="OO199_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5536413.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18607..19611
                     /gene="hflC"
                     /locus_tag="OO199_002411"
     CDS             18607..19611
                     /gene="hflC"
                     /locus_tag="OO199_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5536414.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19693..19890
                     /locus_tag="OO199_002412"
     CDS             19693..19890
                     /locus_tag="OO199_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5536415.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            19994..21292
                     /gene="purA"
                     /locus_tag="OO199_002413"
     CDS             19994..21292
                     /gene="purA"
                     /locus_tag="OO199_002413"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5536416.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21497..21922
                     /gene="nsrR"
                     /locus_tag="OO199_002414"
     CDS             21497..21922
                     /gene="nsrR"
                     /locus_tag="OO199_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5536417.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            21961..24402
                     /gene="rnr"
                     /locus_tag="OO199_002415"
     CDS             21961..24402
                     /gene="rnr"
                     /locus_tag="OO199_002415"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5536418.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24582..25313
                     /gene="rlmB"
                     /locus_tag="OO199_002416"
     CDS             24582..25313
                     /gene="rlmB"
                     /locus_tag="OO199_002416"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5536419.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5921 a         6439 c         7118 g         5878 t
ORIGIN      
        1 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
       61 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      121 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      181 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      241 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      301 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      361 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      421 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      481 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      541 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      601 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      661 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      721 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      781 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      841 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      901 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
      961 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1021 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1081 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1141 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1201 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1261 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1321 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1381 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1441 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1501 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1561 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1621 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1681 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1741 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1801 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1861 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1921 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     1981 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2041 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2101 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2161 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2221 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2281 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2341 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2401 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2461 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2521 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2581 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2641 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2701 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2761 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2821 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2881 agagcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     2941 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3001 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3061 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3121 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3181 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3241 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3301 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3361 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3421 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3481 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3541 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3601 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3661 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3721 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3781 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3841 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3901 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     3961 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4021 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4081 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4141 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4201 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4261 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4321 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4381 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4441 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4501 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4561 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4621 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4681 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4741 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4801 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4861 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4921 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     4981 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5041 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5101 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5161 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5221 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5281 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5341 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5401 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5461 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5521 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5581 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5641 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5701 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5761 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5821 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5881 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     5941 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6001 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6061 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6121 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6181 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6241 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6301 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6361 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6421 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6481 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6541 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6601 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6661 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6721 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6781 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6841 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6901 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     6961 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7021 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7081 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7141 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7201 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7261 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7321 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7381 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7441 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7501 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7561 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7621 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7681 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7741 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7801 gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccaaaatt
     7861 taaaagtgct gtaaggcaca gaccacccaa gcgggaatag ctcagttggt agagcacgac
     7921 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaatt cttctctcaa
     7981 taaaatatct acagcgacgc gatgcgttat tgctggtttt tgttgtctct gacaaactct
     8041 tgtaaacaga gttatccaca gcctcaggct gtaatcttaa tttcaaagaa acttcgcacg
     8101 gtgaatggta ttttttaacc tattgataaa taagttaaaa atcaagattc cgttttgtcg
     8161 agtcgatcac ttgacgattt tattcgtctt gaattgcaat gcgtttttat ttttattcac
     8221 aagctgtgga tgaatcaggc gtcacgcggt aacccttttt caatcacccg aaccagacgc
     8281 tgttttttcg gcaattgcac ttcgactatg cacgcatttc gtctctctat ttgctgcgca
     8341 atcgcccacg ctatgtgctc atcgagaagt gggtgctcac ctttacgact ttccagcgct
     8401 gtcaaaatcg tttcatccca aggggcattg cctaatgcca cggcgatatt acgcagccag
     8461 cgcaggtgac ctatacggcg aatcgccgat ccttccgtga cttttaaaaa cttctcttcg
     8521 ctccaggcaa ataactcaat gagttccggt gcgtgtagcg gcttacgcgg gctgaaatcg
     8581 tcttctgtag tgagttgcga atagcgattc cacgggcaga taagctggca gtcatcgcaa
     8641 ccgtaaatac ggtttcccat taacggtcgc aactcttccg ggagcgcccc ttcaagttcg
     8701 atggtgagat aagagataca gcggcgagca tcgacggtat atggctcgac gatggcaccg
     8761 gtcgggcaaa tcgtcataca ggccacgcat ttgccgcatc cttcctcgac tggttgatcc
     8821 acgggcagcg gaatatcgac cagcaattcg cctaaaaaga agaacgaacc ggcctcgcga
     8881 ttgaggataa gtgagtgctt acctgtccag ccgagcccag ctttttcagc taacgggcgc
     8941 tcgagaatag gcgcagaatc gacaaacggt ctaaaattca gcgaaacaca atgttgctga
     9001 atcatctcgc ccagcttttt gagtcggttg cgcagaagtt tgtgatagtc acggcccagc
     9061 gcataacggc taacatagcc gagtttgggg tttttcagcg tgctggcaaa tgcggcgtta
     9121 gcaggaaggt aattcatccg cacgctgatt acacgcagcg taccgggtaa taactcatgg
     9181 gggcgagcgc gcagcatacc gtgacgtgcc atccagtcca tttcgccgtg gtattgtttg
     9241 tccagccatg cttgcagttt gggctcggac tcgctgagat cggtatcggt aatacctacc
     9301 tgctgaaagc ccagttccag cccccactgt ttaatttttt gcgctaactg attgagatcg
     9361 aggggctctg acatgacgga ccatacaatg aagaaaaacc ccgtaagtat accacacacc
     9421 gtctggtacg ccgacgatat ccgccgcgga gaacgcgagg cggcagatgt gctggggctc
     9481 acactctatg agctgatgct tcgcgctggc gaggccgcat tccaggtgtg tcgttcggcg
     9541 tatcctgacg cccgccactg gctggtgctg tgcggtcatg gtaataacgg cggcgatggc
     9601 tacgtggtcg cgcgactggc caaagcggtc ggcattgagg tcacgttgtt ggcccaggag
     9661 agcgacaaac cgttgccgga agaggccgcg ctggcacgcg aagcatggtt aaacgcgggt
     9721 ggcgagatcc atgcttcgaa tattgtctgg cccgaatcgg tagatctgat tgttgatgcg
     9781 ctgctcggta ccggcttgca gcaagcgccc cgcgaatcca ttagccagtt aatcgaccac
     9841 gctaattccc atcctgcgcc gattgtggcg gttgatatcc cttccggcct gctggctgaa
     9901 accggcgcta cgccaggcgc agtgatcaac gccgatcaca ccatcacttt tattgcgctg
     9961 aaactaggct tgctcactgg aaaagcgcgg gatgttacag gacaactgca ttttgactca
    10021 ctggggctgg atagctggct ggcaggtcag gagacgaaaa ttcagcggtt ttcggcagaa
    10081 caactttctc actggctgaa accgcgtcgc ccgacttcgc ataaaggcga tcacgggcgg
    10141 ctggtgatta tcggtggcga tcacggcacg gcgggggcta ttcgtatgac gggggaagcg
    10201 gcgctacgtg ctggtgctgg tttagtccga gtactgaccc gcagtgagaa cattgcgccg
    10261 ctgctgactg cacgaccaga attgatggtg catgaactga ctatggactc tcttaccgaa
    10321 agcctggaat gggccgatgt ggtggtgatt ggtcccggtc tgggccagca agagtggggg
    10381 aaaaaagccc tgcaaaaagt tgagaatttt cgcaaaccga tgttgtggga tgccgatgca
    10441 ttgaacctgc tggcaatcaa tcccgataag cgtcacaatc gcgtgatcac gccgcatcct
    10501 agcgaggccg cacggttgtt aggctgttcc gtcgctgaaa ttgaaagtga ccgcttacat
    10561 tgcgccaaac gtctggtaca acgttatggc ggcgtagcgg tgctgaaagg tgccggaacc
    10621 gtggtcgccg cccatcctga cgctttaggc attattgatg ccggaaatgc aggcatggcg
    10681 agcggcggca tgggcgatgt gctctctggt attattggcg cattgcttgg gcaaaaactg
    10741 tcgccgtatg atgcagcctg tgcaggctgt gtcgcgcacg gtgcggcagc tgacgtactg
    10801 gcggcgcgtt ttggaacgcg cgggatgctg gcaaccgatc tcttttccac gctacagcgt
    10861 attgttaacc cggaagtgac tgataaaaac catgatgaat cgagtaattc cgctccctga
    10921 tgagcaggca acattagacc tgggcgagcg ggtagcgaaa gcctgcgatg gcgcaaccgt
    10981 aatctatctg tatggcgatt taggcgcagg taaaaccacc tttagccggg gctttttaca
    11041 ggctctgggt catcagggta atgtcaaaag ccccacttat acgctggtcg aaccctatac
    11101 gctcgacaac ttaatggtct atcactttga tttgtaccgc cttgccgatc ccgaggagct
    11161 ggagtttatg gggatccgcg attattttgc caacgatgcc atctgcctgg tggagtggcc
    11221 acaacaaggt acaggtgttc ttcctgaccc ggatgtcgaa atacacattg attatcaggc
    11281 acaaggccgt gaggcgcgcg tgagtgcggt ttcctctgcg ggtgaattgt tgctggcgcg
    11341 tttagccggt taacctttga aaggtggcgg gatgatgtat cgcatcagaa attggttggt
    11401 agcgacgctg ctgctgctgt gcacgccggt gggtgccgcg acgctctctg atattcaggt
    11461 ttctaacggt aatcaacagg cgcggataac gttgagtttt attggcgatc ctgattatgc
    11521 gtttagccat caaagcaaac gcaccgtggc gctcgatatc aaacaaacgg gcgtgattca
    11581 gggactgccg ttgttgttca gcggcaataa tctggtgaag gcgattcgct ctggaacgcc
    11641 taaagatgca caaacgctac ggctggtggt cgatcttacc gaaaacggta aaaccgaagc
    11701 ggtgaagcgg cagaatggca gcaattacac tgtcgtcttt acgattaacg ccgatgtgcc
    11761 gccaccgcct cctccgccgc ccgtggttgc gaaacgcgtt gaaacgcctg cggttgtcgc
    11821 accgcgcgtc agcgaaccgg cgcgcaatcc gtttaaaacg gaaagtaacc gcactacggg
    11881 tgttatcagc agtaatacgg taacgcgtcc ggcagcgcgc gcgacggcta acactggcga
    11941 taaaattatc atcgctattg atgccggaca cggcggtcag gaccctggcg ctatcggccc
    12001 cggtggtacg cgggagaaaa atgtcaccat cgccatcgcg cgtaaattgc gtactttgct
    12061 caatgacgat ccgatgttta aaggcgtttt aacccgtgac ggggattact ttatctcggt
    12121 gatggggcgc agtgatgtgg cacataagca aaacgccaat ttcctcgtgt cgattcacgc
    12181 tgatgccgca ccgaaccgta gtgcgactgg cgcttccgta tgggtgctct ctaaccgtcg
    12241 cgccaacagt gaaatggcca gctggctgga gcagtacgag aaactgtcgg agctgctggg
    12301 tggggcgggt gatgtgctgg cgaacagtca gtctgacccc tatttaagcc aggcggtgct
    12361 ggatttacag ttcggtcatt cccagcgggt agggtatgat gtagcgacca gtatgatcag
    12421 tcagttgcaa cgcattggcg aaatacataa acgtcgacca gaacacgcca gccttggcgt
    12481 tctgcgctcg ccggatatcc catcagtact ggtcgaaacc ggttttatca gcaacaacag
    12541 cgaagaacgt ttgctggcga gcgacgatta ccaacaacag ctggcagaag ccatttacaa
    12601 aggcctgcgc aattacttcc ttgcgcatcc gatgcaatct gcgccgcagg gtgcaacggc
    12661 acaaactgcc agtacggtga cgacgccaga tcgcacgctg ccaaactaag gacgattgat
    12721 gccaattcag gtcttaccgc cacaactggc gaaccagatt gccgcaggtg aggtggtcga
    12781 gcgacctgcg tcggtagtca aagaactagt ggaaaacagc ctcgatgcag gtgcgacgcg
    12841 tatcgatatt gatatcgaac gcggtggggc gaaacttatc cgcattcgtg ataacggctg
    12901 cggtatcaaa aaagatgagc tggcgctggc gctggctcgt catgccacca gtaaaatcgc
    12961 ctctctggac gatctcgaag ccattatcag cctgggcttt cgcggtgagg cgctggcgag
    13021 tatcagttcg gtttcccgcc tgacgctcac ttcacgcacc gcagaacagc aggaagcctg
    13081 gcaggcctat gccgaaggac gcgacatgga cgtgacggtt aaaccggcag cgcatccggt
    13141 gggaacgacg ctggaggtgc tggatctgtt ctacaacacc ccggcgcggc gcaaattcct
    13201 gcgcaccgag aaaaccgaat ttagccatat tgatgaaatc atccgccgca ttgcgctggc
    13261 gcgtttcgac gtcacgatca acctgtcgca taacggtaaa attgtgcgtc agtaccgcgc
    13321 agtgccggaa ggcgggcaaa aagaacggcg cttaggcgcg atttgcggca ccgcttttct
    13381 cgaacaagcg ctggcgattg aatggcaaca cggcgatctc accctgcgcg gctgggtggc
    13441 cgatccaaat cacactacgc ccgcactggc ggaaattcag tattgctacg tgaatggtcg
    13501 catgatgcgc gatcgcctga tcaatcatgc gatccgccag gcctgcgaag acaaactggg
    13561 ggccgatcag caaccggcat ttgtgttgta tctggagatc gacccgcatc aggtggacgt
    13621 caacgtacac cccgccaaac acgaagtgcg tttccatcag tcgcgtctgg tgcatgattt
    13681 tatctatcag ggcgtgctga gcgtgctaca acagcaactg gaaacgccgc taccgctgga
    13741 cgatgaacca caacccgcac cgcgtgccat cccggaaaac cgcgtggcgg cggggcgcaa
    13801 tcactttgca gaaccggcag ctcgtgagcc ggtagctccg cgctactctc ctgcgcccgc
    13861 ctcaggcagt cgtccggctg ccccctggcc gaatgcgcag ccaggctacc agaaacagca
    13921 aggtgaagtg tatcgccagc ttttgcaaac gcccgcgccg atgcaaaaac caaaagcgcc
    13981 ggaaccgcag gaacctgcac ttgcggcgaa cagtcagagt tttggtcggg tactgactat
    14041 cgtccattcc gactgtgcgt tgctggagcg cgatggcaac atttcacttt tatccttgcc
    14101 agtggcagaa cgttggctgc gtcaggcaca attgacgccg ggtgaagcgc ccgtttgcgc
    14161 ccagccgctg ctgattccgt tgcggctaaa agtttctggc gaagaaaaat cggcattaga
    14221 aaaagcgcag tctgccctag cggaattggg tattgatttc cagtcagatg cacagcatgt
    14281 gaccatcagg gccgtgcctt tacccttacg ccaacaaaat ttacaaatct tgattcctga
    14341 actgataggc tacctggcga agcagtccgt attcgaacct ggcaatattg cgcagtggat
    14401 tgcacgaaat ctgatgagcg aacatgcgca gtggtcaatg gcacaggcca taaccctgct
    14461 ggcggacgtg gaacggttat gtccgcaact tgtgaaaacg ccgccgggtg gtctgttaca
    14521 atctgttgat ttacatccgg cgataaaagc cctgaaagat gagtgatatc agtaaggcga
    14581 gcctgcctaa ggcgattttt ttgatggggc cgacggcctc cggtaaaacg gcgttagcca
    14641 ttgagctgcg taaaatttta ccagtagagt tgataagcgt tgattctgcc cttatttaca
    14701 aagggatggt tatcgggacg gcgaagccga acgctgaaga gttactcgcc gcgccgcacc
    14761 gattgctgga tattcgcgat ccgtcgcagg cttactcggc tgctgatttt cgccgcgatg
    14821 cgctggcgga aatggccgat atcaccgcgg cggggcggat cccactgtta gtgggcggta
    14881 cgatgttgta tttcaaggca ttgctggaag ggttgtcgcc gctaccgtcg gcagacccgg
    14941 aagtgcgggc cagaattgag caacaggcgg cagagcaagg ttgggagtca ttgcatcgtc
    15001 aacttcagga ggtagatccg gttgcggcag caaggattca tccaaatgat ccacaaaggc
    15061 tttcccgggc actggaagtt tttttcattt cgggtaaaac tttaacggaa ctgacgcaaa
    15121 cgtcaggaga cgctctaccg tatcaggtgc atcagttcgc catcgccccg gcgagccgtg
    15181 aactgctcca tcaacgcatt gagcagcgtt ttcatcagat gttggcttca ggttttgaag
    15241 cagaagtccg ggcgcttttt gcccgaggag atttgcatac ggacttgcct tccattcgtt
    15301 gcgtgggtta tcgccagatg tggtcttacc ttgaaggcga aatctcatac gatgaaatgg
    15361 tttatcgagg tgtttgcgcc acgagacagt tggcgaagcg gcagataacc tggctgcgtg
    15421 gttgggaagg ggttcactgg cttgacagtg aaaaaccaga acaggcgcgt gacgaagtat
    15481 tacaggttgt tggtgctatc gcaggctgaa tgtgtacaat tgagacgtat cgtgcgcaat
    15541 tttttcagaa tcgaaaggtt caaagtacaa ataagcatat aaggaaaaga gagaatggct
    15601 aaggggcaat ctttacaaga tccgttcctg aacgcactgc gtcgggaacg tgttccagtt
    15661 tctatttatt tggtgaatgg tattaagctg caagggcaaa tcgagtcttt tgatcagttc
    15721 gtgatcctgt tgaaaaacac ggtcagccag atggtttaca agcacgcgat ttctactgtt
    15781 gtcccatctc gcccggtttc tcatcacagt aacaacgccg gtggcggtac cagcagtaac
    15841 taccatcatg gtagcagcgc gcagaatact tccgcgcaac aggacagcga agaaaccgaa
    15901 taaggtttcg ggctgttttt ttacacgggg agccagcgat cctgcgttcc ccgctgatct
    15961 atttagaggg ttatacgctt gtttgaccgt tatgatgctg gtgagcaggc ggtactggta
    16021 cacatctatt ttacgcaaga caaagatatg gaagacctcc aggagtttga atctctggtc
    16081 tcttccgccg gtgtcgaagc attgcaggtg attaccggta gccgtaaagc gccgcaccca
    16141 aagtattttg taggtgaagg taaagcagtt gaaattgcgg aagctgtcaa agcgatgggt
    16201 gcttcggtcg ttctttttga ccatgccctg agcccggcgc aagagcgtaa cctggagcgt
    16261 ttgtgcgagt gtcgtgttat cgaccgcacc ggccttattt tagatatttt cgcccaacgt
    16321 gcgcgtaccc atgagggtaa gttgcaggtt gagctggcgc agctgcgcca tctggctacg
    16381 cgcctggtgc gtggctggac ccaccttgaa agacagaaag gcgggatagg tttgcgtggt
    16441 ccgggtgaaa cccagctcga aaccgaccgt cgtttgttgc gtaatcgcat cgtgcagata
    16501 cagtcgcgcc tggaaagagt tgaaaagcag cgtgagcagg ggcggcaatc gcgtatcaaa
    16561 gccgacgttc ctactgtttc gctggtggga tataccaacg ccggtaaatc tacccttttc
    16621 aatcgcatca ccgaagcgcg ggtctacgcg gcagaccagt tgtttgccac cctcgacccg
    16681 acgttgcggc gtattgacgt tgcagatgtc ggtgaaaccg tacttgcaga taccgtaggg
    16741 tttattcgcc acctgccgca cgatctggtg gcggcattta aagccacgtt acaagagacg
    16801 cggcaagcca cattactgct gcacgtcatt gatgcggcgg atgtgcgtgt acaagaaaac
    16861 atcgaagcgg tgaatacggt tcttgaagag atcgacgctc acgagatccc aaccctgctg
    16921 gtgatgaaca agatcgatat gctggaagat ttcgaaccgc gtattgatcg ggacgaagag
    16981 aacaaaccga tccgtgtctg gctttccgca cagaccggag cggggatacc acagcttttt
    17041 caggctttga cggagcggct ttccggcgag gtggcgcagc atacattgcg tctgccaccg
    17101 caggaagggc gtctgaggag tcgtttttat cagcttcagg caatagaaaa agagtggatg
    17161 gaggaggacg gcagcgtaag tctgcaagtt cgtatgccga tcgttgactg gcgtcgcctc
    17221 tgtaaacaag aaccggcgtt gatcgattac ctgatctaac ggcgtagcgt ctgaagcgtg
    17281 gagtcatatc ctctggcgtc gaaagacaac agggatcacc gcataacaaa tatggagcac
    17341 aaacatggcg tggaatcagc ccggtaataa cggacaagac cgcgacccgt ggggaagcag
    17401 caaacctggc ggcaactctg agggaaatgg aaacaaaggc ggtcgcgatc aagggccacc
    17461 tgatttagat gatatcttcc gcaaactgag caaaaagctc ggtggtctgg gcggcggtaa
    17521 aggcaccgga tctggcggtg gcagttcatc gcaaggcccg cgcccgcagc ttggcggtcg
    17581 tgtcgttacc atcgcagcgg cagcgattgt cattatctgg gcggccagtg gtttctatac
    17641 cattaaagaa gccgaacgcg gcgtggtaac acgctttggt aaattcagcc atctggttga
    17701 gccgggtctg aactggaaac cgacgtttat cgacgaagtc aaaccggtga acgtggaagc
    17761 cgtgcgtgaa ctggccgctt ctggtgtgat gctgacgtcg gacgagaacg tagtgcgcgt
    17821 tgagatgaac gtgcagtacc gcgtcaccaa tccggaaaaa tatctgtata gcgtgaccag
    17881 cccggatgac agcctgcgtc aggctaccga cagcgccctg cgtggagtta tcggtaaata
    17941 caccatggac cgcattctga cggaaggtcg taccgtgatt cgtagcgata ctcagcgcga
    18001 actggaagag acgattcgtc cgtatgacat gggtatcacg ctgctggacg tcaacttcca
    18061 ggctgctcgt ccgccggaag aagtaaaagc ggcgtttgac gatgcgattg ccgcgcgtga
    18121 aaacgaacag caatacattc gtgaagcaga agcgtatacc aacgaagttc agccgcgtgc
    18181 gaacggtcag gcgcaacgta tcctcgaaga ggcgcgtgcg tacaaggccc agaccatcct
    18241 ggaagctcag ggtgaagtgg cgcgctttgc taaacttctg ccggaatata aagccgcgcc
    18301 ggaaattact cgcgagcgtc tgtatatcga gacgatggaa aaagtgttgg gtaacacccg
    18361 caaagtgctg gttaacgata aaggtggcaa cctgatggtt ctgccgttag accagatgct
    18421 gaaaggtggt aacgcccctg cggcgaagag cgataacggt gccagcaatc tgctgcgtct
    18481 gccgccagcc tcttcctcca caaccagtgg agcaagcaac acgtcgtcca ccagtcaggg
    18541 cgatattatg gaccaacgcc gcgccaacgc gcagcgtaac gactaccagc gtcaggggga
    18601 ataacgatgc gtaagtcagt tatcgcgatt atcatcatcg tgctggtagt gctttacatg
    18661 tctgtctttg tcgtcaaaga aggtgagcgc ggtattacgc tgcgttttgg taaggtactg
    18721 cgtgacgatg acaacaaacc tttggtttat gagcctggtc tgcatttcaa gataccgttc
    18781 attgaaacgg tgaaaatgct cgacgcacgt attcagacca tggacaacca ggccgaccgc
    18841 tttgtgacca aagagaagaa agacctgatc gtcgactctt acatcaaatg gcgcatcagc
    18901 gatttcagcc gttactacct ggcaacgggt ggtggcgaca tttcgcaagc ggaagtgctg
    18961 ttgaaacgta agttctctga ccgtctgcgt tctgaaattg gtcgcctgga cgtgaaagat
    19021 atcgtcaccg attcccgtgg tcgtctgacc ctcgaagtac gtgacgcgct gaactccggt
    19081 tctgcgggta cagaagatga agttactacc ccggcagcag ataacgccat tgccgaagcg
    19141 gcagagcgcg taacggctga gacgaagggc aaagttccgg tcatcaaccc gaacagtatg
    19201 gcggcgctgg gtattgaagt tgtcgatgtg cgtatcaagc agatcaacct gccgaccgaa
    19261 gtgtctgaag cgatctacaa ccgtatgcgc gccgagcgtg aagcggtagc gcgtcgtcac
    19321 cgttcacaag gtcaggaaga agcggaaaaa ctgcgcgcga ctgccgacta tgaagtgacc
    19381 agaacgctgg cagaagctga gcgtcagggc cgcattatgc gtggtgaagg cgatgccgaa
    19441 gcagccaaac tgtttgctga tgcattcagt aaagatccgg acttctacgc attcatccgt
    19501 agcctgcgtg cttatgagaa cagcttctct ggcaatcagg acgtgatggt catgagcccg
    19561 gatagcgatt tcttccgcta catgaagacg ccgacttccg caacgcgtta atataacgac
    19621 tgcggtacag gtcaataaag ccaccgcatc ctcagggatg tcggtggttt tctttttcta
    19681 taaggataat gaatgaattc gacaatctgg ctggcgcttg ccctggtttt ggtactggaa
    19741 ggtttagggc cgatgcttta cccgaaggca tggaagaaga tgatctctgc gatgaccaat
    19801 ttgcccgata atattttacg tcgttttggc ggtggacttg tggttgcggg cgttgtggtc
    19861 tactacatgt tgaggaaaac gattggctga acaaaaaaca gactgatcga ggtcattttt
    19921 gagtgcaaaa agtgctgtaa ctctgaaaaa gcgatggtag aatccatttt taagcaaacg
    19981 gtgattttga aaaatgggta acaacgtcgt cgtactgggc acccaatggg gtgacgaagg
    20041 taaaggtaag atcgtcgatc ttctgactga acgggctaaa tatgttgtac gctaccaggg
    20101 cggtcacaac gcaggccata ctctcgtaat caacggtgaa aaaaccgttc tccatcttat
    20161 tccatcaggt attctccgcg agaatgtaac cagcatcatc ggtaacggtg ttgtgctgtc
    20221 tccggccgcg ctgatgaaag agatgaaaga actggaagac cgtggcatcc ccgttcgtga
    20281 gcgtctgctg ctgtctgaag catgtccgct gatccttgat tatcacgttg ctctggataa
    20341 cgcgcgtgag aaagcgcgtg gcgcgaaagc gatcggcacc accggtcgtg gtatcgggcc
    20401 tgcttatgaa gataaagtgg cacgtcgcgg tctgcgtgtt ggcgaccttt tcgacaaaga
    20461 aaccttcgct gaaaaactga aagaagtgat ggaatatcac aacttccagt tggttaacta
    20521 ctacaaagct gaagcggttg attaccagaa agttctggat gatacgatgg ctgttgccga
    20581 catcctgact tctatggtgg ttgacgtttc tgacctgctc gaccaggcgc gtcagcgtgg
    20641 cgatttcgtc atgttcgaag gtgcgcaggg tacgctgctg gatatcgacc acggtactta
    20701 tccgtacgta acttcttcca acaccactgc tggtggcgtg gcgaccggtt ccggcctggg
    20761 cccgcgttat gttgattacg ttctgggtat cctcaaagct tactcaactc gtgtaggtgc
    20821 aggtcctttc ccggccgaac tgtttgatga aactggcgag ttcctctgca agcagggtaa
    20881 cgaattcggt gcaactacgg gtcgtcgtcg tcgtaccggc tggctggaca ccgttgccgt
    20941 tcgtcgtgcg gtacagctga actccctgtc tggcttctgc ctgactaaac tggacgttct
    21001 ggatggcctg aaagaggtta aactctgcgt ggcttaccgt atgccggatg gtcgcgaagt
    21061 gactaccact ccgctggcag ctgacgactg gaaaggtgta gagccgattt acgaaaccat
    21121 gccgggctgg tctgaatcca ctttcggcgt gaaagatcgt agcggcctgc cgcaggcggc
    21181 gctgaactac atcaagcgta ttgaagagct gaccggtgtg ccgatcgata tcatctctac
    21241 cggtccggat cgtactgaaa ccatgattct gcgcgacccg ttcgacgcgt aattctggta
    21301 cgcctggcag atattttgcc tgccgggcta acagtgtgat acattgctgt gtcggttaag
    21361 ccattacgct atccgacaca gtgttaaatc ctcgcttttt tccttccccg aactgaaata
    21421 aattagcgac acagcttgtg gctggtttat catcaatata aatgtatttt ttcccgattt
    21481 cccttttgag gttgatgtgc agttaacgag tttcactgat tacggattac gtgcgctgat
    21541 ctacatggcg tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta
    21601 cggcgtctcc cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt
    21661 gactgctgtt cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg
    21721 tattggtgat gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt
    21781 ttgccacatt acacctgcct gtcggttgaa acaggcgctt tctaaggccg tgcaaagttt
    21841 tcttacggaa ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta
    21901 taaattattg ctggtggagt gacgaaaatc ttcatcagag atgacaacgg aggaaccgag
    21961 atgtcacaag atcctttcca ggaacgcgaa gctgaaaaat acgcgaatcc catccctagt
    22021 cgggaattta tcctcgaaca tttaaccaaa cgtgaaaaac cggccagccg tgatgagctg
    22081 gcggtagaac tgcacattga aggcgaagag cagcttgaag gcctgcgtcg ccgcctgcgc
    22141 gcgatggagc gcgatggtca actggtcttc actcgtcgtc agtgctatgc gctgccggaa
    22201 cgcctcgacc tggtgaaagg taccgttatt ggtcaccgtg atggctacgg ctttctgcgg
    22261 gttgaagggc gtaaagatga tttgtatctc tccagcgagc agatgaaaac ctgcattcat
    22321 ggcgatcagg tgctggcgca gccgctgggt gctgaccgta aaggtcgtcg tgaagcgcgt
    22381 attgtccgcg tactggtgcc aaaaaccagc cagattgttg gtcgctactt taccgaagcg
    22441 ggcgtcggct ttgtggttcc tgacgatagc cgtctgagct tcgatatctt aatcccgccc
    22501 gatcagatca tgggcgcgcg gatgggcttt gtggtcgtag tcgaactgac tcagcgtccg
    22561 actcgccgca ccaaagcggt gggtaaaatc gtcgaagtgc tgggcgacaa tatgggcacc
    22621 ggcatggcgg ttgatatcgc tctgcgtacc catgaaattc cgtatatctg gccgcaggct
    22681 gttgagcaac aggttgcggg gctgaaagaa gaagtgccgg aagaagcaaa agcgggccgt
    22741 gtcgatttgc gcgatttacc gctggtcacc attgatggcg aagacgcccg tgactttgac
    22801 gatgcagttt actgcgagaa aaaacgcggc ggcggctggc gtttatgggt cgcgattgcc
    22861 gacgtcagct actatgtgcg tccgccaacg ccgctggaca gagaagcgcg taaccgtggc
    22921 acgtcggtgt acttcccttc gcaggttatc ccgatgctgc cggaagtgct ctctaacggc
    22981 ctgtgttcgc tcaacccgca ggtagaccgc ctgtgtatgg tgtgcgagat gacggtttcg
    23041 tcgaaaggcc gcctgacggg ctacaaattc tacgaagcgg tgatgagctc tcacgcgcgt
    23101 ctgacctaca ccaaagtctg gcatattctg cagggcgatc aggatctgcg cgagcagtac
    23161 gccccgctgg ttaagcatct cgaagagttg cataacctct ataaagtgct ggataaagcc
    23221 cgtgaagaac gcggtgggat ctcatttgag agcgaagaag cgaagttcat tttcaacgct
    23281 gaacgccgta ttgaacgtat cgaacagacc cagcgtaacg acgcgcacaa attaattgaa
    23341 gagtgcatga ttctggcgaa tatctcggcg gcgcgtttcg ttgagaaagc gaaagaaccg
    23401 gcactgttcc gtattcacga caagccgagc accgaagcga ttacctcttt ccgttcagtg
    23461 ctggcggagc tggggctgga gctgccgggt ggtaacaagc cggaaccgcg tgactacgcg
    23521 gaactgctgg agtcggttgc cgaccgtcct gatgcagaaa tgctgcaaac catgctgcta
    23581 cgctcgatga aacaggcgat ttacgatcca gaaaaccgtg gtcacttcgg tctggcattg
    23641 cagtcctatg cgcactttac ttcgccgatt cgtcgttatc ctgacctgac gctgcaccgc
    23701 gccattaaat atctgctggc gaaagagcag gggcatcagg gcaacaccac tgaaaccggc
    23761 ggctaccatt attcgatgga agagatgttg caactgggtc agcactgttc gatggcggaa
    23821 cgtcgtgccg acgaagcaac gcgcgatgtc gctgactggc tgaagtgtga cttcatgctc
    23881 gaccaggtag gtaacgtctt taaaggcgta atttccagcg tcactggctt tggcttcttc
    23941 gtccgtctgg acgacttgtt cattgatggt ctggtccatg tctcttcgct ggacaatgac
    24001 tactatcgct ttgaccaggt agggcaacgc ctgatggggg aatccagcgg ccagacttat
    24061 cgcctgggcg atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc
    24121 gactttagtc tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag
    24181 aaagcgaaaa aaggcgatgc aggcaaaaaa ggcggcaagc gtcgtcaggt cggtaaaaag
    24241 gtaaactttg agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg
    24301 aagaaagacg cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg
    24361 accaaagcga agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa
    24421 aaagagggtt agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc
    24481 ttatccggcc tacataatca cgcaaattca atatattgca gagatcatgt aggccggata
    24541 aacgtagtgc atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg
    24601 gcatccacgc agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta
    24661 ttttaaaagg ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg
    24721 gcgtggttat ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc
    24781 atcagggcat tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg
    24841 atctgatcgc ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc
    24901 acaacctcgg cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc
    24961 cgaaagatcg ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag
    25021 aaagcgttcc gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag
    25081 agaatatctg gatcgtcggt acggcaggcg aggcggatca tacactctat cagagcaaaa
    25141 tgaccggacg cctggcgctg gtgatgggcg cggaaggtga aggtatgcgt cgcctgaccc
    25201 gtgaacattg cgatgagttg atcagcatcc caatggcagg aagtgtttct tccctgaacg
    25261 tttcggttgc aaccggaatt tgcttatttg aagcggttcg tcagcgcagc tagttcctct
    25321 gaattatgaa aagccatcca gatttggatg gttttt
//
LOCUS       DAJTNV010000058        24652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNV010000058 DAJTNV010000000
VERSION     DAJTNV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(25..597)
                     /locus_tag="OO199_002417"
     CDS             complement(25..597)
                     /locus_tag="OO199_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5536420.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO199_002418"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO199_002418"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5536421.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO199_002419"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO199_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5536422.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO199_002420"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO199_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5536423.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO199_002421"
     CDS             complement(3559..5340)
                     /locus_tag="OO199_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5536424.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO199_002422"
     CDS             complement(5333..7105)
                     /locus_tag="OO199_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5536425.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO199_002423"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO199_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5536426.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO199_002424"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO199_002424"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5536427.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="OO199_002425"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="OO199_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="OO199_002426"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="OO199_002426"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5536428.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="OO199_002427"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="OO199_002427"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5536429.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="OO199_002428"
     CDS             complement(11660..12031)
                     /locus_tag="OO199_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5536430.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="OO199_002429"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="OO199_002429"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5536431.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="OO199_002430"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="OO199_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5536432.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="OO199_002431"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="OO199_002431"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5536433.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="OO199_002432"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="OO199_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5536434.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="OO199_002433"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="OO199_002433"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5536435.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="OO199_002434"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="OO199_002434"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5536436.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="OO199_002435"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="OO199_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5536437.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="OO199_002436"
     CDS             21802..22380
                     /locus_tag="OO199_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5536438.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="OO199_002437"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="OO199_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5536439.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
     gene            24269..>24652
                     /locus_tag="OO199_002438"
     CDS             24269..>24652
                     /locus_tag="OO199_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5536440.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDIN"
BASE COUNT         5790 a         6789 c         5931 g         6142 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 ttataagtaa gcgccccatc agcgacgtct tgtgaaaatt gtcctgtctg gcaacaatcg
    24241 cgcccatcta tattgatgga cacgaacgat gaattcccag acaacaaaag atattccctg
    24301 cttccgttct tatttgcctg atgccctgcg tttaagattt gaagataaac tgaccatccg
    24361 ggccatcgct cagcgtctgg gtctcagtca ttccacaata catacgcttt ttcagcgatt
    24421 tcttgcatcc ggtatcgcat ggccattgcc cgattcagtt tcattcgctc aacttgacgc
    24481 catcctttat gccaacagaa agaaggaatt aacagagcct caaatcagag aaggctcatg
    24541 gcgaaaagaa cggcgaacca gctatagccg tgaatttaag gtccgtctgg ctaagcaggc
    24601 gttacagcct ggggctgttg ttgcccggat cgccagagaa cacgatatca at
//
LOCUS       DAJTNV010000059        24409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNV010000059 DAJTNV010000000
VERSION     DAJTNV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..189)
                     /locus_tag="OO199_002439"
     CDS             complement(<1..189)
                     /locus_tag="OO199_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536441.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTAN"
     gene            713..2641
                     /gene="thrS"
                     /locus_tag="OO199_002440"
     CDS             713..2641
                     /gene="thrS"
                     /locus_tag="OO199_002440"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5536442.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2645..3187
                     /gene="infC"
                     /locus_tag="OO199_002441"
     CDS             2645..3187
                     /gene="infC"
                     /locus_tag="OO199_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5536443.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3284..3481
                     /gene="rpmI"
                     /locus_tag="OO199_002442"
     CDS             3284..3481
                     /gene="rpmI"
                     /locus_tag="OO199_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5536444.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3534..3890
                     /gene="rplT"
                     /locus_tag="OO199_002443"
     CDS             3534..3890
                     /gene="rplT"
                     /locus_tag="OO199_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5536445.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4007..4131
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4013..4057
                     /gene="pheM"
                     /locus_tag="OO199_002444"
     CDS             4013..4057
                     /gene="pheM"
                     /locus_tag="OO199_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5536446.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4340..5323
                     /gene="pheS"
                     /locus_tag="OO199_002445"
     CDS             4340..5323
                     /gene="pheS"
                     /locus_tag="OO199_002445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5536447.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5338..7725
                     /gene="pheT"
                     /locus_tag="OO199_002446"
     CDS             5338..7725
                     /gene="pheT"
                     /locus_tag="OO199_002446"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5536448.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7730..8029
                     /gene="ihfA"
                     /locus_tag="OO199_002447"
     CDS             7730..8029
                     /gene="ihfA"
                     /locus_tag="OO199_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5536449.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8130..9110
                     /gene="btuC"
                     /locus_tag="OO199_002448"
     CDS             8130..9110
                     /gene="btuC"
                     /locus_tag="OO199_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5536450.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9173..9724
                     /gene="btuE"
                     /locus_tag="OO199_002449"
     CDS             9173..9724
                     /gene="btuE"
                     /locus_tag="OO199_002449"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5536451.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9724..10473
                     /gene="btuD"
                     /locus_tag="OO199_002450"
     CDS             9724..10473
                     /gene="btuD"
                     /locus_tag="OO199_002450"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5536452.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10551..10976
                     /locus_tag="OO199_002451"
     CDS             10551..10976
                     /locus_tag="OO199_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5536453.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11223..11936
                     /gene="ydiV"
                     /locus_tag="OO199_002452"
     CDS             11223..11936
                     /gene="ydiV"
                     /locus_tag="OO199_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5536454.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12000..13436
                     /locus_tag="OO199_002453"
     CDS             12000..13436
                     /locus_tag="OO199_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5536455.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13440..13631)
                     /gene="hemP"
                     /locus_tag="OO199_002454"
     CDS             complement(13440..13631)
                     /gene="hemP"
                     /locus_tag="OO199_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5536456.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13763..14809)
                     /gene="aroH"
                     /locus_tag="OO199_002455"
     CDS             complement(13763..14809)
                     /gene="aroH"
                     /locus_tag="OO199_002455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5536457.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14966..15799)
                     /gene="ppsR"
                     /locus_tag="OO199_002456"
     CDS             complement(14966..15799)
                     /gene="ppsR"
                     /locus_tag="OO199_002456"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5536458.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16131..18509
                     /gene="ppsA"
                     /locus_tag="OO199_002457"
     CDS             16131..18509
                     /gene="ppsA"
                     /locus_tag="OO199_002457"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5536459.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18566..20212)
                     /gene="fadK"
                     /locus_tag="OO199_002458"
     CDS             complement(18566..20212)
                     /gene="fadK"
                     /locus_tag="OO199_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5536460.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20269..20562)
                     /locus_tag="OO199_002459"
     CDS             complement(20269..20562)
                     /locus_tag="OO199_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5536461.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20559..21848)
                     /locus_tag="OO199_002460"
     CDS             complement(20559..21848)
                     /locus_tag="OO199_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5536462.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21904..22842)
                     /locus_tag="OO199_002461"
                     /pseudo
     CDS             complement(21904..22842)
                     /locus_tag="OO199_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22862..23626)
                     /locus_tag="OO199_002462"
     CDS             complement(22862..23626)
                     /locus_tag="OO199_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5536463.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23942..>24409
                     /locus_tag="OO199_002463"
     CDS             23942..>24409
                     /locus_tag="OO199_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5536464.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYT"
BASE COUNT         6035 a         5920 c         6420 g         6034 t
ORIGIN      
        1 gtttgcggta aattcctgag taacacagta tgacagcatc gaacttaagt cagaggtatc
       61 aagtagggtg atgagctggc gcgcaaattt attaataggc tctgatgaag gtgttggtat
      121 gtcgctatcg atgtgcaatc tatgaggaga cttataatga gttctgataa tgatatcgtt
      181 ttgactcata atctattcgc cttatatata tctggaggtt ttgaggatga tgattaatta
      241 ttatcgccag aacgaaatgt gcacgaaaaa ttgttataag aattgtgtca cttttatcat
      301 tgtgatatga ccacttcgtc attaatctca tgagcgttta aaatactacc gccaaatcac
      361 gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag taaacctttc
      421 agacgcaccg tgatgttatc agtggttctt taagcgtttt gctggtgtac tcactacaaa
      481 cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta
      541 acatcgttca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt acctgattgc
      601 gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat aaacaaattt
      661 ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata aaatgcctgt
      721 tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt
      781 tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga
      841 actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa
      901 agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcatgcgat
      961 taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta
     1021 ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg
     1081 gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcacga
     1141 agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtttccattc ttgacgaaaa
     1201 catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg
     1261 cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg
     1321 ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg
     1381 ggcagacaaa aaagcactta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga
     1441 ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag cgccgggtat
     1501 ggtattctgg cacaacgacg gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc
     1561 taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct
     1621 gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca catcttctga
     1681 gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca
     1741 ggggctgaag tcttatcgcg atctgccgct gcgtatggcc gagtttggta gctgccaccg
     1801 taacgagccg tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta cccaggatga
     1861 cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt
     1921 agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg
     1981 tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt
     2041 tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg
     2101 tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt
     2161 acagctggac ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga
     2221 gcgtaaggta ccggtaatga ttcaccgcgc aattctgggg tcgatggagc gtttcatcgg
     2281 tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccag ttcaggttgt
     2341 tatcatgaat attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc
     2401 aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg
     2461 cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag aggtggaatc
     2521 aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga
     2581 agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata
     2641 aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat
     2701 tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct
     2761 gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa
     2821 cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa
     2881 gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg
     2941 tcctagtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga
     3001 agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca
     3061 aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg cggtagtcga
     3121 atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa
     3181 acagtaaggc cttcaagtag cactatctgt ggagcctttg ggcttcacag gcattgttcg
     3241 cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac
     3301 cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca
     3361 cgctaacctg cgtcacattc tgactaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc
     3421 gaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata
     3481 agccgttaac gtttttaact ttttaattag aatatagata caggagagca catatggctc
     3541 gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag
     3601 gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg
     3661 gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg
     3721 cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc
     3781 tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca
     3841 aagtagcgtt caccactctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa
     3901 agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac ttttatcgcc
     3961 gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc
     4021 tgctattttc cgcttctttt tttactttag cacctgaatc caggaggcta gcgcgtgaga
     4081 agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg tatgcgcgtt
     4141 ttgaaaaata aaaagccagt ctatcaggcg taattgtcgc agccagtctg gacacggcca
     4201 gtgcggagaa accggagcgt acttaagtac gtgaggattt cgagcactgc ccgggaccaa
     4261 aatggcaagt aaaatagcct gatgggatag gctctaagtc caacgaacca gtgtcaccac
     4321 tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc
     4381 cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa
     4441 aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc
     4501 ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg
     4561 taaagcggaa ctggaaagcg ctgcactgaa tgcgcgtctg gcggcggaaa cgattgatgt
     4621 ctctctgcca ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc gtaccatcga
     4681 ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga
     4741 agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga
     4801 ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct ctggcgtaca
     4861 gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgtgttta
     4921 tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt
     4981 tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc tgcgtaactt
     5041 ctttgaggaa gatttgcaga ttcgcttccg tccttcctac ttcccgttta ccgagccttc
     5101 tgcagaagtt gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg gctgcgggat
     5161 ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc
     5221 cttcgggatg gggatggagc gtctgactat gttgcgttac ggcgtcaccg acctgcgttc
     5281 attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg
     5341 aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgatacgctg
     5401 gcgaatcaaa tcactatggc gggcctggaa gttgacggtg tagaaccggt tgccggtagc
     5461 ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa
     5521 ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgcggtgcg
     5581 ccaaactgcc gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt
     5641 gatttcaaaa ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc
     5701 ttttctgagc tgggaatttc tgacgatcac agtggcatta tcgaactgcc tgcggatgcg
     5761 ccgattggca ccgacatccg cgaatacctg aaactcgatg ataacaccat cgaaatcagc
     5821 gtgacaccaa accgtgccga ctgcttaggt atcattggtg ttgcgcgtga cgttgccgtg
     5881 ctgaatcagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac
     5941 acgttgccga ttacagtcga agcgccggaa gcctgcccgc gctatcttgg ccgtgtggta
     6001 aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc
     6061 gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtactgct cgaactgggc
     6121 cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca
     6181 aaagagggcg aaacgctggt gctgcttgat ggcactgaag cgaagctgaa tgctgacact
     6241 ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac
     6301 tctggcgtga atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg
     6361 tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca ccgttatgag
     6421 cgtggcgttg atccggtact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc
     6481 gacatctgcg gtggtgaggc tggcccggta attgatatca ccaacgaagc aacgctgccg
     6541 aagcgtgcaa ccatcactct acgtcgtagc aaactggatc gcctgatagg ccatcatatt
     6601 gctgatgagc aggtaactga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa
     6661 gacgaatggc aggcagttgc gccgagctgg cgtttcgata tggagattga agaagatctg
     6721 gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca
     6781 agcctgatta tggggactca ccgtgaagct gacctgtcgc tcaagcgcgt gaaaacgctg
     6841 ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag
     6901 cagatgatcc atccaggcgt tgaagcctta ctgctggcaa gcccgatctc tgttgaaatg
     6961 tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagcac
     7021 cgtcagcaga accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc agatactcag
     7081 gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac
     7141 gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgacctt
     7201 gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat
     7261 ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg tattggtttt
     7321 gttggagttg ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg
     7381 ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct
     7441 cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg
     7501 gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt
     7561 gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc
     7621 ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt
     7681 gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac
     7741 aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa
     7801 agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt
     7861 gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa
     7921 cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg
     7981 gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac
     8041 taaaaaggcc gctatgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa
     8101 cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac
     8161 aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc
     8221 tttgcgccgg tgaacaatgg atttcgccag gtgactggtt tactcctcgt ggcgaactgt
     8281 tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg
     8341 ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac
     8401 ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc
     8461 aactccccaa ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc atcactttaa
     8521 tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg
     8581 cattagggat tatctgcagc gcactaatga cgtgggctat ctacttttcc acctctgttg
     8641 atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa
     8701 gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccga
     8761 tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc atgtggttct
     8821 ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg
     8881 gtgctatcgg ctttattggt ctggtgatcc cacatattct tcggttgtgt ggtttaaccg
     8941 atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg
     9001 atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa
     9061 cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag ccgcaaaaag
     9121 acggtctatg attaaaagct aagattttta ctgaccacac ctaggagaaa cgatgcaaga
     9181 ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta
     9241 cgccggtaat gtgctgttaa ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata
     9301 tgagcagttg gagaatattc aaaaagcctg ggccgatcga ggttttgtgg tgctgggatt
     9361 cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg
     9421 taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg
     9481 acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga
     9541 gagcggattc tatgcccgta tggtcagcaa aggccgtgca ccgctgtacc cggatgatat
     9601 tttatggaat tttgaaaaat tcctggttgg cagggacgga aaagtcatcc agcgtttttc
     9661 cccggatatg acgccggaag atcccattgt gatggaaagc attaaactgg cgttggcaaa
     9721 ataatgtcta ttgtgatgca gttacaggat gttgcggaat ctacccgcct ggggccgctt
     9781 tcaggcgagg ttcgggctgg ggaaatcctg cacttggtgg ggccgaatgg cgcgggtaag
     9841 agtaccttac tggcgcaaat ggccggaatg accagcggta agggaagcat tcagttcgcg
     9901 gggcaaccac tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc ctatctttca
     9961 caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac
    10021 gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa
    10081 ctcggtcgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg
    10141 gtggtgttgc aaatcacacc acaagccaat cccgcaggcc aattgctgct tcttgatgag
    10201 ccgatgaaca gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct gagcgcgctg
    10261 tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac gttgcgtcat
    10321 gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag
    10381 gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc
    10441 gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcccga aaatcgggta
    10501 attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct
    10561 gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc
    10621 caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa
    10681 gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcgatcgt ttcgatttgc
    10741 atctgccccg tgaaaccaaa caacaagcct ctattggcac gcaaattgat aaagacgagt
    10801 tgctgcctgg tgacctggtc tttttcaaaa cgggttccgg acaaaatggt ttgcatgtag
    10861 gtatttacga taccaacaac caatttatcc acgcctctac cagcaaggga gtgatgcgtt
    10921 cctcacttga taatgtctat tggcagaaaa atttctggca ggcgagacga atctagtatg
    10981 tcgaaaaatt gacggcgatg atacatagcc gtcaatttac tcctgaatac agtagtattt
    11041 tgcagacaaa aattccgtaa atggattatt cgtttataat caggataatc taaaaaaagg
    11101 ccccctgaaa aagtggttaa catggaacaa atgaaagtta ctccttctgt aattgttatg
    11161 tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga gggacggtta
    11221 ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg ccgataagag
    11281 ataaccagca actccttgtc ggcgttgagt taattaccca tttctccagt gaagatggca
    11341 cggttcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa cattggcaat
    11401 tattttcaga gcagttggaa ttactaaaat catgccaaca cttttttatt caacataaac
    11461 tctttgcctg gcttaattta acgccacaag tggctacatt attattagac agggataatt
    11521 acgcaggcga actattaaaa tatccgttta ttgagctgtt gattaatgag aattacccgc
    11581 atctcaatga aggaaaagat aatcgcgatc ttttgtcctt atcgcaaatg tatccccttg
    11641 tgttaggcaa tttgggagca ggtaatagca caatgaaagc ggtttttgat ggtcttttca
    11701 cccgcgtaat gctggagaaa ggttttattc agcaacaaat cacgcatcgc tccttcgaac
    11761 cgtttattcg tgcgatccag gcgcaaattt ccccttgttg caattgcatc atcgcggggg
    11821 gcattgatac gccagaaata ttggcacaga ttaccccctt cgatttccat gctctccagg
    11881 gatgcctgtg gcctgccgtt cctataaatc agataacgac gttggttcag cgataaccct
    11941 cctgtttgct ggtgtttaag acgagagtaa ccgtctacac tatcaaacag ataggatcta
    12001 tgaccctgtc ttttattacc cgctggcgcg atgaattgcc agaaacctat acagcacttt
    12061 cccctacgcc tttaaataat gcccggctga tttggcataa taccgaactc gctaacacgc
    12121 tgagtattcc atcgtcgctg tttaaaaatg ccgcaggtgt ctggggcggc gaaaccctac
    12181 tgcctggtat gtcaccactg gcacaggttt acagtggtca tcagttcgtc gtctgggcgg
    12241 gccaactggg tgatgggcgc ggcattttac ttggcgaaca actgcttgct gatggcacta
    12301 caatggactg gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg ggtgatggac
    12361 gggcggtttt acgttcgacg atacgagaaa gtctcgccag tgaggcgatg cattatctgg
    12421 gcattccgac gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg tatcgggaaa
    12481 cggtggagcc aggcgcgatg ctgatgcgtg tggcaccaag tcatctgcgc tttggtcatt
    12541 tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg tcagttggct gactttgcca
    12601 ttcgtcatta ctggtcacat cttgaagacg atgaggacaa ataccgtctc tggtttaacg
    12661 atgttgtcgc acgtaccgcg tcgttaattg cccaatggca gacggtcggc tttgcacatg
    12721 gggtgatgaa taccgacaac atgtcgctgc tgggactgac gcttgattac gggccgtttg
    12781 gttttcttga tgattacgaa cccggtttta tttgtaatca ctcggatcat caagggcgtt
    12841 atagctttga taatcaacct gctgtcgcgt tgtggaattt acagcgtctg gcgcagacat
    12901 tgtcaccatt tgttgccgta gatgccctga atgaggccct ggacagctat cagcaggttt
    12961 tgttgacgca ttatggacaa cggatgcggc agaaactggg cttcatgacg gagcaaaaag
    13021 aggataacgc gctactgaat gaattattca gtctgatggc gcgagagcgc agcgattata
    13081 cccgcacatt ccgcatgctg agtctgaccg agcagcacag cgcggcgtca ccgctacgtg
    13141 aggagtttat tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg gggcgtttgc
    13201 aacaagacga ggttagcgat agtgagcgtc agcaactgat gcaaagcgtt aacccagctc
    13261 tggtattgcg caactggttg gcgcaacggg cgattgaagc ggcagaaaag ggtgatatga
    13321 tggaattgca ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga gatgatgact
    13381 atgtcagtcg tccacctgac tggggtaaac ggctggaagt cagctgctcg agttaaaggc
    13441 tacttagtta acagcagctt gccagcctga gttttacgga gcagatattc ttgcccgtca
    13501 tgatcgataa tcagtttgcc atccggacct aacagtgtct ggctggtaat tcgccggatg
    13561 ggctgcgggg aagcggtctt gtgattggca tccgtttcag gcgtgagttt tctgctatcc
    13621 gtataacgca taaaaattaa taataattga tataacaatg atttctatta tcaatattat
    13681 ggcaggccag agcaagcagt aatgtgttga gaagacaaca agttgcgcag aaatgcccat
    13741 cccatcagga atgggcacgc attcagaagc gggtatctac cgcaaaggcg agtttttcga
    13801 ccaggcgttc ggtatcttcc cagcccagac acgggtcggt aatggattga ccgtaagtga
    13861 gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc
    13921 cagcaatctc cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact
    13981 ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta
    14041 aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt
    14101 ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca
    14161 tctgaccatt tttgtctggc gagaggaaca tatggctggc gcgggctgcg cggatagcat
    14221 ccacggcaat ccgcgtgttg ccatcggtac catttttaaa acctaccgga caggagagtg
    14281 ctgaagccat ttcgcggtgg atctgacttt cggtagtacg agcgccaatc gcgccccaac
    14341 tgattaaatc agcaataaac tgaccggtta ccatatcgag gaactcagtc gcggtaggga
    14401 cacccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc
    14461 gatagctgcc gtttaaatcc ggatcggaga ttagcccttt ccagccgaca acagttcgtg
    14521 gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg ttgcgcagcg
    14581 actgcagacg ggtggcgtac tctattgcag cggtgagatc gtggatcgag caggggccaa
    14641 taatgaccaa cagtcgctta tcttcaccat tcagtatttt ttcaattctg cggcgggagt
    14701 cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg
    14761 ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca
    14821 gaaatttgtt gcttctacgg caggactacc ggaaagtgat gcgcatcacg ataaaccaat
    14881 cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa
    14941 aaaaacaaac caatgcacta gttctctagt acattcggcg actaaggccc atgatatcga
    15001 ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat
    15061 ttttacggta caaggcttcc acttccgcga cttccatcct gcactgacga agcgaggcat
    15121 agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg tcgatagtca
    15181 ggccgaacaa tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat
    15241 cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa ttgcattgcc agatacagac
    15301 tggtgggggt tttaccacag cgagaaacac cgagcaggat cacctgagcc tgatccagat
    15361 tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat
    15421 catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat
    15481 ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaagc
    15541 cttcactttg caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg
    15601 ggcgcacgcc tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc
    15661 tctcattttc gacaaacggc agcgtgatgc tgctgatagt gacgggaaat tgtgacatta
    15721 ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc
    15781 gatcaacagc attatccatt ttgttcttcc cgtgaagcag acataattct atgaaagcat
    15841 aaattaaaag cgcacagaag cgtaggacgt aatgtctggt ttataaaatg aaccttcatt
    15901 ttatttttta taaaaacagc atttcatttt tatggtttcg tttataccga tggtttatgt
    15961 ggaaattgtc gaatagagca ggtttgcgca acgctgggat cagtcttaaa aagtaaaaaa
    16021 atatatttgc ttgaacgatt caccgttttt ttcatccggt taaatatgca aagataaatg
    16081 cgcagaaatg tgtttctcaa accgttcatt tatcacaaaa ggattgttcg atgtccaaca
    16141 atggctcgtc accgctggtg ctttggtata accaactcgg catgaatgat gtagacaggg
    16201 ttgggggcaa aaatgcctcc ctgggtgaaa tgattactaa tctttccgga atgggtgttt
    16261 ccgttccgaa tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg gaccaaagcg
    16321 gcgtaaacca gcgcatttat gaactgctgg ataaaacgga tattgacgat gttactcagc
    16381 ttgcgaaagc gggcgcgcaa atccgccagt ggattatcga cactcccttc cagcctgagc
    16441 tggaaaacgc catacgcgaa gcctatgcac agctttccgc cgatgacgaa aacgcctctt
    16501 ttgcggtacg ctcctccgcc accgcagaag acatgccgga cgcttccttt gccggtcagc
    16561 aggaaacctt cctcaacgtt cagggttttg acgccgttct cgtggcagtg aaacatgtat
    16621 ttgcttctct gtttaacgat cgcgccatct cttatcgtgt gcaccagggt tacgatcacc
    16681 gtggcgtggc gctctccgct ggtgttcagc ggatggtgcg ctccgacctc gcatcttctg
    16741 gcgtgatgtt ctccattgat accgaatccg gctttgacca ggtggtgttt atcacttccg
    16801 catggggcct tggggaaatg gtcgtgcagg gtgcggttaa cccggatgag ttttacgtgc
    16861 ataaaccgac actggcggcg aatcgcccgg ctatcgtgcg ccgcaccatg gggtcgaaaa
    16921 aaatccgcat ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa atcgaagacg
    16981 taccgcagga acagcgtgac atcttctcgc tgaccaacga agaagtgcag gaactggcaa
    17041 aacaggccgt acaaattgag aaacactacg gtcgcccaat ggatattgag tgggcgaaag
    17101 atggccacac cggcaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg cgctcacgcg
    17161 gtcaggtcat ggagcgttat acgctgcatt cacagggtaa gattatcgcc gaaggccgtg
    17221 ctatcggtca tcgcatcggc gcgggtccgg tgaaagtcat ccatgacatc agcgaaatga
    17281 accgcatcga acctggcgac gtgctggtta ctgacatgac cgacccggac tgggaaccga
    17341 tcatgaagaa agcatctgcc atcgtcacca accgtggcgg tcgtacctgt cacgcggcga
    17401 tcatcgctcg tgaactgggc attccggcgg tagtgggctg tggagatgca acagaacgga
    17461 tgaaagacgg tgagaacgtc actgtttctt gtgccgaagg tgataccggt tacgtctatg
    17521 cggagttgct ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg gatctgccgt
    17581 tgaaagtgat gatgaacgtc ggtaacccgg accgtgcttt cgacttcgcc tgcctgccga
    17641 gcgaaggcgt gggccttgcg cgtctggaat ttatcatcaa ccgtatgatt ggcgtccacc
    17701 cacgcgcact gcttgagttt gacgatcagg aaccgcagtt gcaaaacgaa atccgcgaga
    17761 tgatgaaagg ttttgattct ccgcgtgaat tttacgttgg tcgtctgact gaagggatcg
    17821 cgacgctggg tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct gattttaaat
    17881 cgaacgaata tgccaacctg gtcggtggtg agcgttacga gccagatgaa gagaacccga
    17941 tgctcggctt ccgtggcgcg ggccgctatg tttccgacag cttccgcgac tgcttcgcac
    18001 tggagtgtga agcagtgaaa cgtgtgcgca acgacatggg gctgaccaac gttgagatca
    18061 tgatcccgtt cgtgcgtacc gtagatcagg cgaaagcggt ggttgaagaa ctggcgcgtc
    18121 aggggctgaa acgtggcgag aacgggctga aaatcatcat gatgtgtgaa atcccgtcca
    18181 acgccttgct ggccgagcag ttcctcgaat atttcgacgg cttctcaatt ggctcaaacg
    18241 atatgacgca gctggcgctc ggtctggacc gtgactccgg cgtggtgtct gaattgttcg
    18301 atgagcgcaa cgatgcggtg aaagcactgc tgtcgatggc tatccgtgcc gcgaagaaac
    18361 agggcaaata tgtcgggatt tgcggtcagg gtccgtccga ccacgaagac tttgccgcat
    18421 ggttgatgga agaggggatc gatagcctgt ctctgaaccc ggacaccgtg gtgcaaacct
    18481 ggttaagcct ggctgaactg aagaaataaa ataaatcccc ggcggcgttc agtcgccggg
    18541 gttatgtgat ccccgaagat gaaacttatt caatctcttc acagacatcc tgcgttaatc
    18601 gccgtataat atcttttctt aacaaaaact tttgtatttt atctgaggca gtgcgcggta
    18661 gtttttcgat taccacgata tgttcaggat atttatattt tgcgacccgt ttacggctaa
    18721 aaaaagccgc catctcttcc agcgataatg aatgatgcgg cgctttcagc acgacataag
    18781 cgcatgatcg ttcacctaaa cgttcatcgg gcattgcaac cacacaggca tcgtgaattt
    18841 taggatgctg caataaaata tcttccactt cacggctgct aatattttcg ccgccgcgga
    18901 caataatatc ttttttgcgt ccggtaattt ttatatagcc agcctcatcc atacggcaga
    18961 gatcgccgct gtaataccag ccttcttcat ccagcgcacg ggcggttaat tcaggttcat
    19021 caaaataccc cataaacaca ttggggccac gcgatgcttc ttcaccttcg caacctggcg
    19081 gtaaggtctt gcgtgcgtca tcgaccactt taatctctac acctgcggca gcgtaaccat
    19141 cggtatgcat aaagcgcgac aaagaattat cgagattcac caccgcatgc ggcgaacttt
    19201 ctgtggaacc atagacactt aataatttaa tgctgcgctg ctggcattcg cgcgccactt
    19261 ttttggggat tgtggtaccg ccgcaaagaa agaagcgcag cgctgaaagg tctgcgggtt
    19321 gtttctctag taaattcaaa agatcataga caaacggcgt tgcgcctagc atacaggtgc
    19381 aacgctgctg ctcaagcagc gcgagacacg catcaggagt gaaaatatct aacaacacgc
    19441 tgcgagcgcc aattaagaat ggtgccgtta cgccatgcag aaagcccgtt gcgtgaccaa
    19501 gtggcgcagg catcataaag acatccagcc aggtcagatt cagccgcgcg caataagccc
    19561 gctcactggc gagaatattg ttatgcgtta gcatcacgcc ctttggcaga ccctcggttc
    19621 cggaggtaaa cagcaccgca gctaattcat cgccgtgggt cgttatcgcc gtggtcagtg
    19681 ggatattgtc ggcgataatc tgacttaatg agagggaaga ggtggcggga gccagtttgt
    19741 ccacgccgac aatttgttgt agttgtggaa gctgattttg cagcggcagg attaaatcta
    19801 ccggacgcgt ttgtttaaac aacgtcggtg caaagaacat ttttgcctga cacttattga
    19861 gcacccacac cagttctgct tcccgccagg aaggcaacag cggcacggaa accgcaccga
    19921 ttttcaggca ggcaagatag ataacggtaa attcacacca gccaggcagt tgaaatgcga
    19981 tgcgatcgcc tgattcaata cccttcgcta acatccagtt tgccagacag ctcgcggcgt
    20041 gatcgagcgc gctataggtg tacgatgcac catgattatc gaccacggca attttgtctg
    20101 gcatcgcacg agcggtctgc tgccagtaat cggccagtga agcatcgccc catagccctt
    20161 gctgacgata cgccgcacga cgttgttcgt taaacgttaa tgtcactttc attttggact
    20221 ctcgaaacag aacatcaggc ccgagttgcg ggcctgtggg atgcatgatt agccgtagcg
    20281 aaaatcaata ccgaaggtgc ctgcgggata ttgccactgt tcgagaatag tgttaccgca
    20341 cagcacccga caggtgccgc actccagaca accggcggga tcaaaatgaa tgtttcctac
    20401 gtcatcctgc ttataaagtc cggcagggca ggctttcatt aatttacgga attcattgat
    20461 atcgggattt tccgtcagaa tgatatgcgg gtggccctca tcaacatgga atttattgac
    20521 gcctaatttg atgtcgacgt taaccgtagc gttctggctc atagcgcggt tgctccctta
    20581 atgccatctt tcagcaagtt gatcagccca attttcttcg cgtgtcccat gatcattttg
    20641 cgtaccggct ggtttggttt gccgtcaatg gtgaacatct cgttcatgat gtcggctacc
    20701 attcgtgggt attggctaaa caggcgcggg ttttccatca gcgccgggat cttgcgaaaa
    20761 tgctgcatat cacgcatgac gcagctttgt tccagctcac gtttgtattg cgccagactg
    20821 ttggcggaga aatccgcgcg ttctttggcg gcgatcaccg ttgtggcggc agcctgagcc
    20881 gatgcaatgg ctaaatccat gccgcggacc gtaaaaccca aattcaggca gaagcctgcg
    20941 gcgtcaccaa cgatcatcac gccctcgtta accagttgcg gcaccattgc cagaccgcct
    21001 tccggcacca tatgcgcgga atattcaagc agtttgccgc cgctaatcag cgggcgaatg
    21061 gcggggtgtt gtttaaaatc ttccagcatt tgcggcacgc ttttttgcgc atgggcgata
    21121 tcacccaatc cacaaaccag ccccaaggat atggaatcct tgttggtata gagaaatccc
    21181 ccgcccatca ggccgtcaga aggggaaccg gcaaacagcc aggcggcacc ttcctcgccc
    21241 gtaatattaa agcgatcgtt gatctgttct ggtgtgaggc caataacctc tttaacacca
    21301 acagcgtaat gatgcggatc ggaagcggga accattccca gcgagcggcc aagcatcgag
    21361 ttaacgccat cagccagaat caccacattc gcttcgagaa tatcatcccc agcctgcacg
    21421 ccagtgacct tgtttccttc acgaaccagc gcgtcgacgc gaacgcctgg gataaactgc
    21481 gcgcccgcct gctcggcttg ttccatcaac cacgggtcca gacgattacg caatacggta
    21541 taagatgcgt gttgaggaac atctggttgc tcgcggtgaa aatcgagggt aacggcgctt
    21601 tcttcggtta agaaggagat tttctcgcgt gtgaccttgc gttctaccgg cgctgatact
    21661 gcaaagcctg gaatgattgc ttcaagtgtg tgggcataaa gacgcccgcc ggtcatgttt
    21721 ttacatccgg cactgtcgcc gcgttctatc accaggacat ccagcccggc tcgtgccatg
    21781 accagtgcgg caacgctacc agcaacgccc gcaccgacca caatggcatc aaatttgtca
    21841 tccgacatgc gttatgctcc cttcctgctg cgtttacagc gggagatgtg gttgaaaggg
    21901 gaattagcgg ctcaactggc taatcagggc agggacgact ttgtagatat cgcccaccag
    21961 accgtagtcg gcatagttga agattggcgc atttttatct ttattgatgg cgacaatcac
    22021 ttttacgccg ttgccgccaa ccatatgttg gatctgcccg gagatcccca gcgtcaggta
    22081 gagatcggat ttcagcaaca cgccggagac accgatataa cgttcacgct ccatccagtt
    22141 ctcgccttcg gcaattggac gtgaacagcc gacttcagca ttcagcaccg ccgccagttc
    22201 gtggaccatt ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa ccacacgttt
    22261 tgctttgctc aggtccacgc tgcttgcggc tttcgcacgg cgttcgcgac agagaatttc
    22321 atgacgcgga gcaacataag gtaccgtttc tgtcgggcac tgatgagagg tatcactggt
    22381 gcacggttcc tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt tgatcttttc
    22441 ctaagcgaac gctaacccgc catacatccg gtgttcggcg caaatgtgac catcgacaat
    22501 atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt tgcacactta accgtgctgc
    22561 cagagcttta ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt tatctttcag
    22621 cagggcagca atgctttcgg cgtaattttc agtgcgttgc agcgcgtcgt tttgctcaag
    22681 aacataaata cattttggac cataaggcat aactgcctgc gcctggtcgg tattttgtac
    22741 aatggcatac acctgttggc cccattgctg agcgccgcca aacagttcag cataacgttc
    22801 aggattatcg ctaaagaccc agacgctgtt taattgactc atcgtttctt ctccgtaggc
    22861 ttcagttcag ggctttcttc agatgttccg ccagctcggc aatggcctcc ggcgaatcgt
    22921 tatcgagaat gatgtgctta cgttctgttt gcggcggtac gcgaatgccg accagttcag
    22981 caagtggcgc gctctggctc cagtcaatat cacttgcctg ccactgattt accggttttt
    23041 tacccgcgcc gagaatggct ttcatcgaag gaatgcgtgg cacgttaata tcggaggtga
    23101 cgcagagcac ggctggaaca gagagttcaa taacttcaac atcatcttca agcgtgcgtt
    23161 caatcaccag tgtattgccc tgacgctgaa tagcactcac tgcattaatc accggaagtt
    23221 gcagaatttc tccgaccagc aagccaacct gctgggcata aaggtcgccg gaaccttcac
    23281 caaagatcag taaatcgaag ccgatctttt caattgctgc cgccagcgct tttgcggtat
    23341 cgagaggcag tgcatgttca agttgcgcat cctgcaccaa atacaggctg tgcggcccgc
    23401 gggatagcac gtctttgcgc actttcgagt tctgcaacaa tgagccgcca acggtcagcg
    23461 cggctatctc atcgtcatct gttgcgagct ggcttgcagc ttcaatggca ttgagatcga
    23521 actggctgat tttggcgtcg gcattgtcga aattcagggt gtattctgga gtgacaacaa
    23581 tgtcctgttc ttcaggcacc agcttaaagc aggttattat tttcatgatt tctcctgtga
    23641 gtcgcatacg gaaccatctc cgatggaagg taaaaatggc ctgaaaatag tgtgcgatag
    23701 cggtgcgggg ggttcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    23761 gcgcttgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    23821 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    23881 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    23941 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24001 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24061 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24121 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24181 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24241 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24301 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24361 aagtaaaaat agccaaacat cttcgttatg ggatgcattt gcctataca
//
LOCUS       DAJTNV010000060        22726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNV010000060 DAJTNV010000000
VERSION     DAJTNV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="OO199_002464"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="OO199_002464"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5536465.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="OO199_002465"
     CDS             691..1317
                     /locus_tag="OO199_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5536466.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="OO199_002466"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="OO199_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="OO199_002467"
     CDS             3452..4090
                     /locus_tag="OO199_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5536467.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="OO199_002468"
     CDS             4154..4822
                     /locus_tag="OO199_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5536468.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /locus_tag="OO199_002469"
     CDS             4819..5604
                     /locus_tag="OO199_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5536469.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /locus_tag="OO199_002470"
     CDS             5608..6420
                     /locus_tag="OO199_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536470.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..8036)
                     /locus_tag="OO199_002471"
     CDS             complement(6432..8036)
                     /locus_tag="OO199_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5536471.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(8162..8467)
                     /locus_tag="OO199_002472"
     CDS             complement(8162..8467)
                     /locus_tag="OO199_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5536472.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8575..8651)
                     /locus_tag="OO199_002473"
     tRNA            complement(8575..8651)
                     /locus_tag="OO199_002473"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8615..8617),aa:Val,seq:gac)
     gene            complement(8656..8732)
                     /locus_tag="OO199_002474"
     tRNA            complement(8656..8732)
                     /locus_tag="OO199_002474"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8696..8698),aa:Val,seq:gac)
     gene            9040..10296
                     /locus_tag="OO199_002475"
     CDS             9040..10296
                     /locus_tag="OO199_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5536473.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10337..11710)
                     /gene="mdtK"
                     /locus_tag="OO199_002476"
     CDS             complement(10337..11710)
                     /gene="mdtK"
                     /locus_tag="OO199_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5536474.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11925..12566
                     /gene="ribE"
                     /locus_tag="OO199_002477"
     CDS             11925..12566
                     /gene="ribE"
                     /locus_tag="OO199_002477"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5536475.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12606..13754)
                     /gene="cfa"
                     /locus_tag="OO199_002478"
     CDS             complement(12606..13754)
                     /gene="cfa"
                     /locus_tag="OO199_002478"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5536476.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14045..15256)
                     /locus_tag="OO199_002479"
     CDS             complement(14045..15256)
                     /locus_tag="OO199_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5536477.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15369..16301
                     /locus_tag="OO199_002480"
     CDS             15369..16301
                     /locus_tag="OO199_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5536478.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16298..17323)
                     /gene="purR"
                     /locus_tag="OO199_002481"
     CDS             complement(16298..17323)
                     /gene="purR"
                     /locus_tag="OO199_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5536479.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17622..17711
                     /gene="ynhF"
                     /locus_tag="OO199_002482"
     CDS             17622..17711
                     /gene="ynhF"
                     /locus_tag="OO199_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5536480.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17877..19046
                     /locus_tag="OO199_002483"
     CDS             17877..19046
                     /locus_tag="OO199_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536481.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19192..19773)
                     /gene="sodB"
                     /locus_tag="OO199_002484"
     CDS             complement(19192..19773)
                     /gene="sodB"
                     /locus_tag="OO199_002484"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5536482.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19901..20728)
                     /gene="mepH"
                     /locus_tag="OO199_002485"
     CDS             complement(19901..20728)
                     /gene="mepH"
                     /locus_tag="OO199_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5536483.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21063..21410
                     /gene="grxD"
                     /locus_tag="OO199_002486"
     CDS             21063..21410
                     /gene="grxD"
                     /locus_tag="OO199_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5536484.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21449..21949)
                     /gene="tnpA"
                     /locus_tag="OO199_002487"
     CDS             complement(21449..21949)
                     /gene="tnpA"
                     /locus_tag="OO199_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5536485.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            21957..>22726
                     /locus_tag="OO199_002488"
     CDS             21957..>22726
                     /locus_tag="OO199_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5536486.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTV"
BASE COUNT         5901 a         5285 c         5870 g         5670 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat taactggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taactgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgctttaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct ttactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 ccaggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcatccc ctgttttctg taattgctcg
     6661 cgtagcccag ggaaaggaat atctttcact ttaagcggac gctgtccgcg ggcatcaata
     6721 aaaacgtcaa acgagtagct gttgtcttcc gttttcagga cggtgcgcga ctcattaatt
     6781 tccatttcgt agtcttcacc gagggcgaga atatgaatga tccccgcttc acgtaaggcc
     6841 agtagtcgac gaatagactc tgaagggatt gccgcataat tatcgatgaa aaccctggca
     6901 aggcctttac tgaaccgttt atggtcgtgt tcattcagat gaggaacaat ttcctgtacg
     6961 gcttcatgca ggcgcagaat gacataacgc cagggaacag tatgtttttc tcgtttattg
     7021 cgttcgactt cctggagatt tttttctgcc cagtcaaatg ggtcgcgttg tttgcgctcg
     7081 gcaaaccagg cttgagcaaa ggaatcgaca ttcaggctct ctaaggctat gcgttggctc
     7141 cagtctggat cagcaaactt gatttcctct actatcaatc taaatacccg atccaaaagg
     7201 ccctcttcac ctttttgaat ctcagcattt aatgcctgat cggtgacgat gtgtaagggc
     7261 tcgtagggaa tagggcaata gaaatcggct tcgggtaaaa tacccgtgcg cgacatcaac
     7321 gtaatattta gcttttcact tgcgttatcg cggtgaaaaa cgacgtgttg tttatcatct
     7381 tcaatgaacg aaccatgctg aatagccact gccattgccg catccagtcc gctcaaggat
     7441 gttcccataa tacccacgtt acacgcatcg acctttgctt ccatcaggcc tgaccacggg
     7501 ctgggaaaat acgttctggt tgcttcttct tcatcaggcc agacgtgacc cgtggcaatc
     7561 accgctaaat caaacgtctc gctgggtaaa tcctgatttg tagcgagcat gacgccagca
     7621 tttgtaattt gcagatcggt aacctggcat gattcataaa cagccactgc aaatttttgc
     7681 tgtcgtgcct ggtctactaa tcgtaaaaat tgatcgcgga aatattcgcc cagcagaatt
     7741 cgcggtaaaa actgacgatc gtgcaaggtt tcttttttaa cgccataacg ctggagatgg
     7801 ctggcttctt gcttttgtag ccattcgaga tacgtacaat taatcggcgg tatttcaata
     7861 ctggcaatat ttgccagcat catttttgag ttttcctcat cactgtatgg catcccgaca
     7921 ccggcctcgt cagcctgctc gaagatagaa atagaaagtg gagtttgttg ctgtagaagc
     7981 gagaataagg tgtagatccc cgtaggcccg gcacccacaa tagcaatttt tttcattgtt
     8041 acttacctgt acgcattctt tcgaatttag tgttatcaga taagtttggt tgagattttt
     8101 agaatttgag aaatttcaga cagggatgaa aagggcgcta accgcccttg attaaattct
     8161 gtcaggcgag ttttgcctga ttgatttgac taagtggctc attgacatcg aaaactttgg
     8221 cgacaacttc ctcaacgccg agatttttca gtcgggcagt atgtttttcc agataggcaa
     8281 gggcgctttt ttcatcagtg aaaaggtaga tcccaccggc ttcgtggttc ttttcacttt
     8341 ctgtccatac cttccacaga aaaccaggtt cctgattaat cgactcagca agtggtttaa
     8401 gctgctcagc cattgcgtcg ccaaatgggc cattaaaagc aaaatgaagt tgtaacaaag
     8461 ttgccatagc gattctcctt ctgataacgc atacgaagac agtgtaaatc ttttaacgta
     8521 ttggatacag agaagaaaca gagggaaaaa ggaaaagacc agattaagaa aatctggtgc
     8581 gtccgagtgg actcgaacca ccgaccccca ccatgtcaag gtggtgctct aaccaactga
     8641 gctacggacg caggatggtg cgttcaattg gactcgaacc aacgaccccc accatgtcaa
     8701 ggtggtgctc taaccaactg agctatgaac gcaacgttgt aggtgacaac ggggacgaat
     8761 attagcggca gagtggaaag gtggcaagag gcaaaacgta attttctgcg ctatttcgac
     8821 cgtttgcaga gcttttaagc aaattggcta tattttgttg atttgcaagg gcgattttta
     8881 ttcaggatcg catttacatc tgatacaacc caataattcg tgtatcagag tttaaacaga
     8941 gttgtttgcc accatctcaa atctgtcgtg gataaatcct ccgtagcctt tacacttagg
     9001 tagcaataaa ggcacacaga tgcagcaaag gaataaatca tgggatctga tgcgaaaaac
     9061 ttgatgagcg acgggaatgt gcaaattgtt aagaccggcg aggtccttgg cgcgacgcaa
     9121 cttactgaag gcgagttgat tgttgaagct ggcggaagag ccgaaaatac cgtggtcacg
     9181 ggggctggct ggttgaaagt ggcaaccggt gggatcgcca aatgcacaca gtacggtaac
     9241 aatggcacgc tatcggtcag cgacggtgcc attgccacag atattgttca gtccgaggga
     9301 ggcgcaatta gtctctctac gctcgctacg gttaatggcc gccatcccga aggtgaattc
     9361 agcgttgatc agggttatgc ctgcggtttg ttgctggaaa atggcggtaa cctgcgtgta
     9421 ctggaaggac atcgcgcgga aaaaatcatt ctcgatcaag agggcggcct gttggttaat
     9481 gggacaacct cagcggtcgt ggtagatgaa ggtggtgaat tgttggtgta tccaagtgga
     9541 gaagccagca attgtgagat taatcagggc ggcgttttta tgctggccgg gaaagccagt
     9601 gatacgttgc ttgctggtgg caccatgaat aatctcggtg gtgaagactc tgacactatt
     9661 gttgagaatg gatccattta tcgtctgggg acggatggcc ttcagctcta cagttccggt
     9721 aagacgcaaa acctgtcggt gaatgtgggg gggcgggctg aagtgcatgc cggtacgctg
     9781 gaaaatgcgg taatacaagg tggaacagtg atcctgttgt cacccaccag cgcggacgaa
     9841 aattttgtcg tagaggaaga tcgcgcaccg gttgaactga ccgggagtgt tgcattactg
     9901 gacggcgctt caatgattat tggttatggc gcagagctgc aacaatcaac gattactgta
     9961 cagcagggcg gtgtgttgat tctcgacggc agtacggtaa aaggtgacag cgtcactttc
    10021 attgttggta acatcaatct gaatggcgga aaactgtggc tgatcactgg tgcggcaacg
    10081 catgtgcaac tgaaagtgaa acgcctgcgc ggagagggag cgatttgcct gcaaaccagt
    10141 gcgaaagaaa tttcacctga cttcatcaat gtgaaagggg aagttaacgg ggatatacgc
    10201 gttgagataa cagatgccag tcggcaaact ctgtgtaacg ctctgaaatt acagccagac
    10261 gaagacggga ttggcgcaac gctccagcct gcgtaaaaac acaggcgcaa cccgtgaagg
    10321 ttgcgccttg tctttattag cgggacgctc gttgcagaat gatggctgac ggcatacgtt
    10381 gcaggaaccg catacgcagc atcatcataa tggctgccga tgtcaggccg ataataaagc
    10441 ctatccagaa gcctgctggc cccataggtt caacgaccag atcggtcagt gccagaatat
    10501 agccgcttgg caagcccagc acccagtaag ctgtaaaggt gatatagaaa atggaacgcg
    10561 tatctttata accacgcaaa atcccactgc caatcacctg gattgagtca gaaatctgat
    10621 ataccgccgc cagcaacatc aaatgcgcag ccagcgttac aacctcggga ttgtcgttgt
    10681 acaacagggc gatttgctcc cgcagtgaaa ccgtgaaaat ggccgtcagg gttgccatac
    10741 agacacccac cataagcccg gtccgcgcag cggtttgcgc atccagcgtt gagccctgac
    10801 ccagacgata acctacgcgg atagttaccg ctgccgccag cgacattgga agcacaaaca
    10861 tcagtgaact aaagttcagg gcaatctggt gtcctgcgac atcaacaata ccgagcggag
    10921 acactaacag agccacgacg gcaaacagtg tcacttcaaa gaacagtgcc agcgcaatcg
    10981 gcaaaccgag ttgaatcagt cgtttcataa ccgcaggctc gggcttagcg gtgccttttt
    11041 cgttacgaat atcgcgcatg gagcgggcgc gtttaatgta agaaaccatg gcaaggaaca
    11101 tgacccaata caccgccgca gtagccacgc cacaaccaac gccaccgagc tcaggcatac
    11161 cgaaatgacc ataaataaag atatagttca ccgggatgtt caccagcagg ccgataaagc
    11221 ccattaccat acccggcttg gtttttgcca gaccttcaca ttggttacgg gcaacctgga
    11281 agaacagata tcccggcgcg ccccacaaca acgcacgcag ataacccacg gctttgtccg
    11341 ccagagccgg atcgatgttt tccatgtagc ggataatgta acctgcattc cacagcacca
    11401 gcataatgag aacggaaaca aaacctgcca gccagaaacc ttgtcgcacc tgatgcgcaa
    11461 tgcgctcacg tcgaccggaa ccatttaatt gcgcgataac cggcgttaat gccagcagca
    11521 gtccgtgacc aaagaggatc gccggaagcc agatagaagt accgatagcg accgccgcca
    11581 tgtcggtggc actatagccg cccgccatca cggtatcgac aaaacccatc gcagtttggg
    11641 cgatttgcgc gagaatcacc gggattgcta atgctaataa cagacgcgct tcactgatat
    11701 acttctgcac gtgaacacct tttatttgta gttatatgag agactaaaaa gccgcctgaa
    11761 tgggcagcaa aaagaagaag caggggaaat tcagtctatt gtagcgagga tttattaatt
    11821 ctccagtgaa aaaatagcca ttcttacccc ttcactggta agggcgtttt ccacctgcta
    11881 ttgtgctgaa cagaatgtct taactgattt caggagttgt aagtatgttt acggggattg
    11941 tacagggcac cgcaaaactg gtgtcgattg acgagaaacc aaattttcgt acgcatgtgg
    12001 tggagttacc cgaccacatg ctggacggcc tggaaaccgg tgcttccgtg gcgcataacg
    12061 gttgctgcct gaccgtgacg gaaattaacg gcaaccatgt cagttttgac ctgatgaaag
    12121 aaacgttacg cattaccaat cttggcgatt taaaagtggg ggattgggta aacgttgagc
    12181 gtgcggcgaa attcagtgat gaaattggcg gacacttaat gtcaggtcat attatgacca
    12241 ccgctgaagt ggcgaaaata ttaacctcgg aaaataatcg ccagatctgg tttaaagtcc
    12301 aggatagtca gttgatgaaa tatatcctgt acaaaggatt tattggcatc gacggtatta
    12361 gcctgaccgt cggcgaagtc acgccaacgc gtttttgcgt ccatttaatt ccggaaacac
    12421 tggaacgcac gactcttggg aagaaaaaac ttggcgcacg cgtcaacatt gaaatcgatc
    12481 cacaaactca ggcagtggta gatacggtag aacgtgtgct ggcggcacga gaaaatgcca
    12541 tgaatcaacc aggtacagaa gcctgatctg aactcgcccc cgaagagggg gcgatagaat
    12601 agcctttagc gagcgactcg aaggccgttt tcaacaccgc gtgagaacac cacctgccag
    12661 agctgaatat cacgggcgcg gaaagcacct gcgcaggcat tcagataata ggtaaacatc
    12721 cgtttaaagc gttcactgta gttatccgca atttctggcc atgcggcgag gaatcgttca
    12781 taccacgcca tcaacgtagt atcgtaatca gcaccgaagt tatgccagtc ttccatcaca
    12841 aagtggggtt cgctggattg agcaatctgg cgtacagagg gcaggcaacc gttcggaaaa
    12901 atatatttat taatccaggg atcaacattc agatcggttt ttttcgaacc gatagtatgg
    12961 agcaggaata taccttccgg tttcaaatta cgatccacca ccgcaaaata ggtatcgtaa
    13021 tttttcggtc cgacgtgctc gaacatcccc acagaaacaa tacgatcaaa ctggtcgttc
    13081 aggtcacgat aatcttgcag caaaatggtg acatccaggc ctttacagcg ttcctgagcc
    13141 attttttgct gttcggcaga aatggtgacg ccgaccacgc ttacgtcata gttagatgcc
    13201 atgtagtgcg ctagtccgcc ccagccgcag ccaatatcca gtacgcgcat ccctggtttt
    13261 aactgcaatt tttcacaaat cattttgagc ttcgcctgct gggcagattc cagattatcg
    13321 gcatctttcc agtaagcgca ggaatattgc atgaagggat caagcatgcg gctgaacaag
    13381 tcattaccca aatcgtaatg ctccttgccg actatccagg cacgtttttt actctgcaga
    13441 ttgaagagac gagcgccggc aatacgcagc gtgtctttga aatgatgggg gagttggttc
    13501 tcgagacctg cgcgtaagac tttgctaaaa aacatatcca gtcggtcaca ttcccaccag
    13561 ccatccatat aactttcgcc taaccccaaa gagccttcct gcagaacgcg tttaaaaaaa
    13621 tcggggtttt tcacacgaat atccgccggg gcagaaccgt taatggctat accggcacgg
    13681 ctaagtaatt cgttggcgat acggtaccag ttgtcatccg gtacactgac ttcttctata
    13741 cacgatgaac tcatagtttc tccatcactg gtcggtgatc agaaccttaa aacagcgtag
    13801 acgctttttg ggctttgtga gaaatctcgc agaaaaaacc gcgagtctat aatccgacgt
    13861 agaacagagg gaaaggagga acccttgccg aaatgaccat ccatagtgag tcgggaacga
    13921 tcctgtcccc gtaaaaacat aatatttatc gtagctttaa caactcgaaa ttagtatagg
    13981 ccgctaattt caatcataca acaaaatttt gttagcagcc taagtataag tatatcgata
    14041 tagatcagtg tgattcgcta tgagcgactt cggcattgcc atgattcggg cagtcaactt
    14101 cttcacaacg ttgcatcatg taacccagcg cgaccagcac tactgttgat aacatcacgc
    14161 tggtggtggt gagcaatggc gtgctgatac taataagcca ggaaactacc agacttgcga
    14221 ggaagcacag acccagttgc agagtgttct gcaacgctgc ggcgcgaccg gttgcatgtg
    14281 ggaagggacg cagcgcctgg gcgacaacaa tagggtagat cgcgccattg gcaatcgcca
    14341 tcacacagaa tgggatcagg atttcgacca gcgacacatg gctaatgaag cccgcagccc
    14401 aggtcgcaat gacgctgaca gcaaacagca ccagcaacca cggtaataac tgcttgcctt
    14461 gccatttctg cagtgcggcg cgacagccat aaccaccaat cagaaacgcg atagtttgcg
    14521 ggacataact taaaccaata actgccgggc tgtagcccat ttcactaagg atgaacggtg
    14581 aaccggtcag ccatgcaaaa aaactggctg aacaggctgc gtatatcagc acgttgccgc
    14641 gataggtttt agaacgtagc aggtcggtaa aggtcagacc atcctgacta ttgttacggg
    14701 ccttcgtcgt gggtttgagc cagaaaatag gcagaatcag caccacggta atggcaaaca
    14761 gggtggcgaa aatcgcctgc caggaaaaat ggaccagcag ccagcttcct aacaggggag
    14821 ccagtgccgg agacagaccc accagcggca tgatggtcgc aaaaatacgg ttgactttct
    14881 gtgaaggata ataatctgtc actaatgctt gccagataac cgccgcggcg cagaccccca
    14941 cagcctgtac aaaacgcaat accagcagcg tggcggcgtt ttctacccac agcatcccca
    15001 gactacctaa cgcaaaaatt gtcaggccga ttaataacac cggtttacga ccataacggt
    15061 cggagagtgg cccccacaga agctgggctg cggcaaaacc ggcaaggaac agactaaggc
    15121 tggcactgac agcagacgca ggcgtttgca ggtcggcctg tatggcggcg aaagcaggca
    15181 gatacatatc ggttgccaga aaaccgagta cgctcaaacc cgccagccag actaaaaatc
    15241 ttttcccagg ttgcatgtat attctctaaa tctctcaatt aaacaccgcc gcaaagtgta
    15301 gggagtgaaa ttagtgttgt gaaacggtaa tatttgatga ctggtttcaa aaattttgca
    15361 ggcagactat gtggtcagaa tattcactcg aagttgttga tgcggtagcg cgtaatggta
    15421 gttttagcgc tgcggcacag gagctgcatc gtgttccttc tgcggtcagc tataccgtgc
    15481 gtcagctgga agagtggctg gcggtaccgc tctttgaacg acggcaccgt gatgtggagc
    15541 tgaccgctgc tggagcgtgg tttctcaaag aagggcgctc tgttgtcaaa aaaatgcaga
    15601 tcacccgcca gcaatgtcag cagatagcga acggctggcg cggtcagtta gctatcgcag
    15661 tggataatat tgtcaggcca gaacgtacac ggcagatgat cgttgatttt tatcgccatt
    15721 ttgatgatgt cgaacttctt gtctttcagg aagtgttcaa cggtgtctgg gatgcgcttt
    15781 ccgacgggcg cgtggaactg gcaattggcg caacacgcgc gattccggta ggcggtcgtt
    15841 atgccttccg ggatatgggg atgctaagct ggagttgcgt tgttgccagt caccacccgc
    15901 tggcgttgat ggatggcccg ttcagcgatg atacgttgcg caactggccg tcgttggtgc
    15961 gcgaagacac ctcgcgaacg ctacctaaac gtattacctg gttgctggat aaccaaaaaa
    16021 gacttgtcgt acctgactgg gaatcatcgg caacctgtat ctcggcagga ttatgcatag
    16081 ggatggtgcc aacacatttc gccaaaccgt ggcttaatga ggggaagtgg gtagcacttg
    16141 aactggaaaa ccccttcccg gattcggcat gttgtctgac atggcagcaa aatgatatgt
    16201 cgcctgcgtt aacctggctg cttgagtatc tcggcgatag tgagacgctg aataaggagt
    16261 ggctacggga gccggaagag actcccgcaa cgggtgatta acgacgatag tcgcggaacg
    16321 ggccgtcagc cacggagcgg cgttcaatca aacgcggatg cacttcaatg gactgcggtt
    16381 cttcacgttt gttgacgata cgatccaaca gcatgttgaa cgctgtttca cccagcgaat
    16441 cttttggctg atggatcgtg gtcagcgccg gcgtaaaata gcgcgcgttg cgcacgttat
    16501 cataaccgat cagcgaaaca tcctgcggga cgcgcaggcc catttcatca gcagcacaaa
    16561 gtgcgcccat tgccatgata tcgccaccac agaagacggc agtagggcga tgcggctgcg
    16621 acaggatttg ctgcatggcg cgataaccgg attcaggttc aaagtcaccc tgcacaatcc
    16681 agctttccgg caccttgatc atcgcttctt ccatcgcctt cataaaaccg gcaaggcggc
    16741 ctgcgccggt gttacgttcc agcgggccgg ggatgacgcc gatttcgcgg tgaccgcgtt
    16801 caatcagata acgcccggcc atgtagccgc cttcgaacgc gttatcaatg accgcatcgg
    16861 tgaagtcagc ttttgcttca ccccagtcca tcaccaccat tgggatatgg cgatactctt
    16921 ccagcatcgc cagcaacggc tctgggtact cagaacacat caccagcaga ccatcgacgc
    16981 gtttttgcgc catcatcgac agataagccc gctgtttctc aagattgttc cacgcattac
    17041 ccagaatcag ggtgtaacct ttctggaagc aatttttttc aactgcttca atgatctcgg
    17101 caaaataggc cgcttcgctg ctggtcgcca gcaaaccgat agacttggtg tggttaacct
    17161 tcaggctacg cgccaccgcg ctaggggagt agtgtaattc tttaatcgct gcccacacgg
    17221 cgttgcgcgt ttcttcagcg acgaaacgtg ttttgttgat cacgtgtgac acagttgtag
    17281 tggaaacgtt tgctcgtttc gctacatctt ttattgttgc cattccattt cactccagac
    17341 cctaacttca gctcctgcaa aatcgcaagg taaacgtttg cctttacaca ccttacgcac
    17401 aacaaaagtg ttgcggtacg ccggaaaaac gacacggagc gtcaggaagg ggtcaatggc
    17461 cagtacgcaa atagtgtaag cgtggaattt tggccgatct tgacgaaaag gggaagtggt
    17521 aaaaacactt atcattataa atgacgggag attttttcat cacagtgtgt aaaaatgcgt
    17581 aatatcactt tttgtgtggt aataaaaaag gagaaaactt gatgagcacc gaccttaaat
    17641 tttcactggt aacaacgatt atcgtcctcg gtttgatcgt agccgtgggt ttgactgccg
    17701 cgctgcactg atttcctctc gagggagcgc gttctccctc gcatctcctt cctgtttgtt
    17761 agcaaaatgg caaaagtgtt ttctcttttt gttattttca tttttttgtg attgctgtca
    17821 tgctttcgcc actttaatga atatcgcgta atgacgcgaa gaggtggaga taaggcatga
    17881 aaattaacta tccgttgctg gcgctggcga ttggcgcgtt tggtatcggg acaacggagt
    17941 tctcgccaat gggcttgttg cccgtcattg cgcgcggtgt ggatgtctcg attcccgctg
    18001 ccggaatgtt aatcagtgcc tatgcagttg gcgtaatggt gggcgcgccg ctgatgacgc
    18061 ttctactttc tcatcgtgcc cgccgcagtg cgttgatttt cctgatggca attttcacgc
    18121 taggcaacgt tctttccgcc atcgcgccgg attatatgac cctgatgctt tcacgtattt
    18181 tgaccagcct gaatcacgga gcattttttg gtttgggttc agtcgtggcc gcaagcgtgg
    18241 tgccaaaaca taaacaggcc agcgcagttg ccactatgtt tatggggtta accctggcaa
    18301 atatcggtgg cgtgccggcg gcgacctggt tgggtgaaac catcggctgg cggatgtcat
    18361 ttctggcaac ggcggggctg ggagtgattt caatggtaag tctgttcttc tcattaccta
    18421 aaggtggtac aggggcacga cctgaagtga aaaaagagct ggcggtatta atgcgtccgc
    18481 aggtgctgtc tgcattgctg acgacggtac tgggagctgg tgcaatgttt accctctaca
    18541 cctatatctc tccggtactg caaagtatta cccacgcaac accggtgttc gtcacggcaa
    18601 tgctggtgct gattggtgtc ggattctcta tcggtaacta tctcggcggc aaactggcag
    18661 atcgttcagt taacggcacg ttgaaaggct ttttgttgct gctgatggtg attatgctgg
    18721 caatcccgtt cctggcccgc aatgagttcg gcgcagctat tagcatggtg gtgtggggcg
    18781 cagcaacctt tgcggttgta cctccgttac agatgcgcgt gatgcatgtc gccagtgaag
    18841 cgccaggtct gtcttcatca gtcaatattg gtgcctttaa tcttggaaat gcgctgggag
    18901 cagctgctgg tggtgcggta atttccgctg ggctgggata cagctttgtg ccggtgacgg
    18961 gggcgattgt cgcgggactg gcattattgc tggtgtttat gtcagccaga aaacaacctg
    19021 aaacagtttg cgttgctaac agctaattaa acaaaaacgc agaaggataa ccttctgcgt
    19081 tacagttgct gacaacgtgc gatttatctt gccgaatgcg ctgttaacgc ctcatccgcg
    19141 ttggcatggt aagcgaagcg catcaggcaa tgctgcattt gccatcagtt attatgcagc
    19201 gagatttttc gctacgaatt cccagttcac cagcgcccag aagtgctcca gatagccagg
    19261 acgtgcattg cgatagtcga tgtaataagc gtgttcccag acatcaacgg tcagcagcgg
    19321 agtcgcatcg gtggtcagcg gagtacccgc gttagaggtt gaaacgatag ccagtttgcc
    19381 atcgctgttt ttcaccagcc aggtccagcc agaaccaaag tttttgatcg ctgcatcagt
    19441 aaactgcgct ttgaaatcgg caaagctgcc aaaagatgcg gcgatagctt cagcgacttt
    19501 tccagtcggt tcgccaccgg cgttcggtgc caggcagttc cagtagaaag tatggttcca
    19561 gacctgagct gcgttgttga atacgccacc ttcagagctg cgaataatct cttccagtga
    19621 tttaccttca aacgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg
    19681 atgcttgccg tagtgatact cgatagtttc cgcagaaatg tgcggtgcca gagcatcttt
    19741 agcatatggt agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc
    19801 atacgtacaa tagccttatt gtgcgtatgg atagggtagc aaattaaaag gtgagacaaa
    19861 agatgaactt accctgttgc cgtaacaaca gggtaaagtt ttagcgaagt gtttttgggg
    19921 tcattacccg acgagcgcca acatagtggc gctgccagta gtcttcactg agagaagtga
    19981 tttgaatttc ctgacctgtg cgcggtgact gaataaattt gccgttgccg acatacacgc
    20041 cgacatgatc ggctgtaccg cgtccctgag tacggaaaaa gaccaggtcg ccgtttttca
    20101 gctcactacg ttcgataggt cctgcatcac gcaggtgata catttcattc gccgtacgcg
    20161 gaatacgaat tttcaccaaa tctttataag cgtaataaac caggccgctg caatcaaaac
    20221 cggtatgcgg tgagctgccg ccccaacgat atggcttacc aatttgctgc atcagtttat
    20281 tcattgccac tttcgtggct ttttgcacct tcgctttgtg cgcgtcggca agggttcctg
    20341 acgcggcatt tttgactttc acacaatgcg atttatagcc cttacgtttg gtacactttt
    20401 cggtaaagct ggctgaggcc gttttattga cggcgtgttt gctgcgagtt ttggcgtttt
    20461 tagaagcggt aatggaagat tttttaacag aagaagcggt tttgcttttg gtcatacttt
    20521 tactggcggc ttttttcgct gttttctggc tggctttctg ggtctttttg gtggttgccg
    20581 tgcttttctt tttatctgct tttttggtga tatgagtggt agcagaactc tccctggctt
    20641 gctttgaagc atgggccata ggcgttaaag gcagggtggt aaaaagtaaa gcacagagcg
    20701 tgatcgaaat acggtttatc cgcgccactg agcgtccctc tgttaaggca ggtctatcat
    20761 gcctgcgtct gatttacaaa acttgatgat tctaatgcat agaagaagag aaagttaata
    20821 gcttttattg ttaaaaaata cgtttcgttg gcgagcgcaa aaaaatgcag cagtgaaagc
    20881 taaaaatggt tataaatacg ccagtttgcc gcgcatcaga gaagaaacga atatttgaaa
    20941 tgcaacaaat agagcaaagg gataaaatag caaaagcgtg atgaaaatgt tattttccga
    21001 tgccgcactg acccgctaca atgtcagatt actgccgtaa agaagtttga ggaagcaaga
    21061 caatgagcac cactatcgaa aaaatccaac gccagattgc tgaaaacccg atcctgctgt
    21121 acatgaaagg ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca gtccaggcgc
    21181 ttgccgcatg tggcgaacgt tttgcctatg ttgatattct gcagaatccg gacattcgtg
    21241 cggaactgcc gaaatatgct aactggccga ccttcccgca actgtgggtt gacggcgagc
    21301 tggtcggcgg ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga
    21361 tcaaagaaac tgccgctaaa tacaagtctg aagagccgga cgcggaataa tccgtcctgc
    21421 aatactgacg gatgcgaacg ggcatccgtc agtcctttat ccggtgcgtc gtaaaacctc
    21481 gtccttcagg gcggggatat aaggcgcggt tttccacctg accaggtgtt tgctgttgtt
    21541 caatgtactg gcggatgacg gatattggcg caccttcgca gctactggca aaatagccag
    21601 gactccacaa aacgcctttg tagtaatacc tgactgcaat atctggtcga tctcgtcgca
    21661 gtaacctatt cgatatgcct ttgaggctgt ttaccagact ggatatcgcc agtttgggag
    21721 gatagttaat taacaaatgg acgtgatctg gttcgccatc catttcaacc agttcagctg
    21781 caaaatcagc acatacattt gaaaagtaag tgcgtagttt ttctgtcgtg tcatggtcaa
    21841 aaatctggcg tcggtatttg gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat
    21901 gccttccacg ccggatatcg gtttcttttt tcacagacca aagtatcatt tgacctgtga
    21961 aacgattaca ggcatttaaa ttccagttaa gaccaggtgg tcaacaggag cgtgaaatga
    22021 ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcgctt cagaatgaga
    22081 atcatgaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg ctggttgagt
    22141 ggaaaaacgc cactgaaacg caatggctta aagattcgcc ctcacagcca ttgcaacagt
    22201 cactgaaaga ccttgagcgg gcttacaaaa acttcttcca gaagcgggct gcttttcccc
    22261 gattcaaaaa gcggggacag aatgctgcat tccgctaccc gcaggacgtt aagctcgatc
    22321 aggaaaacag ccgtattttt ctgccgaaac ttggctggat gcgctaccgg aatagccgtc
    22381 aggtcacggg agttgtgaaa aatgtcactg tcagccagtc ctgcggtacg tggtacatca
    22441 gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca atgatcgggc
    22501 tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gagcctgtaa
    22561 acagttttca aaaaaaccag aagacgctgg cgagacttca gcgacagtta agccggaagg
    22621 tcaaattcag caacaactgg cagaagcaga aacgcaaaat acagcgactg cattcctgta
    22681 tcgcaaatat ccgcagggac taccttcaca aagtcacaac gaccgt
//
LOCUS       DAJTNV010000061        22610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNV010000061 DAJTNV010000000
VERSION     DAJTNV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..423)
                     /gene="pgrR"
                     /locus_tag="OO199_002489"
     CDS             complement(<1..423)
                     /gene="pgrR"
                     /locus_tag="OO199_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5536487.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERF"
     gene            561..1481
                     /locus_tag="OO199_002490"
     CDS             561..1481
                     /locus_tag="OO199_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5536488.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1481..2416
                     /locus_tag="OO199_002491"
                     /pseudo
     CDS             1481..2416
                     /locus_tag="OO199_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2434..3072
                     /locus_tag="OO199_002492"
     CDS             2434..3072
                     /locus_tag="OO199_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5536489.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3363..4091
                     /gene="mpaA"
                     /locus_tag="OO199_002493"
     CDS             3363..4091
                     /gene="mpaA"
                     /locus_tag="OO199_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5536490.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4066..5025)
                     /gene="ycjG"
                     /locus_tag="OO199_002494"
     CDS             complement(4066..5025)
                     /gene="ycjG"
                     /locus_tag="OO199_002494"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5536491.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5144..5650
                     /gene="tpx"
                     /locus_tag="OO199_002495"
     CDS             5144..5650
                     /gene="tpx"
                     /locus_tag="OO199_002495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5536492.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5694..7235)
                     /gene="tyrR"
                     /locus_tag="OO199_002496"
     CDS             complement(5694..7235)
                     /gene="tyrR"
                     /locus_tag="OO199_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5536493.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7383..8444)
                     /locus_tag="OO199_002497"
     CDS             complement(7383..8444)
                     /locus_tag="OO199_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5536494.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8441..9838)
                     /locus_tag="OO199_002498"
     CDS             complement(8441..9838)
                     /locus_tag="OO199_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5536495.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9994..10992
                     /locus_tag="OO199_002499"
     CDS             9994..10992
                     /locus_tag="OO199_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5536496.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11103..11975)
                     /gene="ompG"
                     /locus_tag="OO199_002500"
                     /pseudo
     CDS             complement(11103..11975)
                     /gene="ompG"
                     /locus_tag="OO199_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12048..13129)
                     /locus_tag="OO199_002501"
                     /pseudo
     CDS             complement(12048..13129)
                     /locus_tag="OO199_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13143..13802)
                     /gene="pgmB"
                     /locus_tag="OO199_002502"
     CDS             complement(13143..13802)
                     /gene="pgmB"
                     /locus_tag="OO199_002502"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5536497.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13799..16066)
                     /gene="ycjT"
                     /locus_tag="OO199_002503"
     CDS             complement(13799..16066)
                     /gene="ycjT"
                     /locus_tag="OO199_002503"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5536498.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16063..16686)
                     /locus_tag="OO199_002504"
     CDS             complement(16063..16686)
                     /locus_tag="OO199_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5536499.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16683..17105)
                     /locus_tag="OO199_002505"
     CDS             complement(16683..17105)
                     /locus_tag="OO199_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5536500.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17127..17902)
                     /locus_tag="OO199_002506"
                     /pseudo
     CDS             complement(17127..17902)
                     /locus_tag="OO199_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17920..18972)
                     /locus_tag="OO199_002507"
     CDS             complement(17920..18972)
                     /locus_tag="OO199_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5536501.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19003..19845)
                     /locus_tag="OO199_002508"
     CDS             complement(19003..19845)
                     /locus_tag="OO199_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5536502.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19832..20713)
                     /locus_tag="OO199_002509"
     CDS             complement(19832..20713)
                     /locus_tag="OO199_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5536503.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20734..22017)
                     /locus_tag="OO199_002510"
                     /pseudo
     CDS             complement(20734..22017)
                     /locus_tag="OO199_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22031..>22610)
                     /gene="ycjM"
                     /locus_tag="OO199_002511"
     CDS             complement(22031..>22610)
                     /gene="ycjM"
                     /locus_tag="OO199_002511"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5536504.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5571 a         6081 c         5510 g         5448 t
ORIGIN      
        1 aaaacgttca gaaacgacat cggtcaaacc ataatcaatg gtaagctgaa tatcaatttc
       61 aggatgcgat ttcaggaatg tgcgcatggc tggtaacaat atcgtttttg ctgcatgttc
      121 tacagtagta atacgtattg tcccggatgg gcggttctgc agatcgctca gggatgtcag
      181 ggcagaatct atgtcatgca atatagggcc aagaacagac aaaagatgct cgcccgcttc
      241 agtgggtaca acgctgcgcg tggttcgcgt cagaagccgc aatcccaatc gttcttctat
      301 acggcgcact atctggctta aagctgactg caccatgctc aggcgggctg ctgcgcgagt
      361 gaagctacgc tcctctgcaa cgacgacaaa cgccatcaga tcagcgattt cttcacgctt
      421 cattcagtat ctccatacaa tctattaatc atttaaatga taggtctaat catgatttag
      481 ggtctaatca acttaatgat caatatctat agtgatatca ccaaccgtca aaacgaaccg
      541 acaactccat gaggaactga atgatgaata ataaagtcag cttcactaac agcaataatc
      601 caaccatctc tttgtctgca gtgatctatt tccctccaaa atttgatgaa acccgtcagt
      661 atccggcaat tgtggtatct cacccaggtg ggggcgttaa agagcaaacg gccggaacct
      721 atgccgaaaa gctggcggaa aagggatttg tcactattgc ttatgacgca tcttatcaag
      781 gtgaaagtgg cggcgagccg cgtcagttag aaaacccata cattcgtact gaagacgtca
      841 gtgccgtgat tgattacctg acaacgcttt cttatgtcga caatacccga attggtgcga
      901 tgggaatttg tgccggtgca ggatatacag ccaatgctgc aattcaggat cgccgcatca
      961 aggccatcgg taccgttagt gcggtcaata ttggttcgat gttccgcaat ggttgggaaa
     1021 acaatgtgaa atctattgat gctctgccac acattgaagc tggctcaaac gcccgaacca
     1081 gcgatattag cagcgaagaa tacgccatta tgccactggc tccaatgaag gaggccgatg
     1141 cgccgaacga agaactgcgt caggcctggg agtattacca cacacctcgt gcgcagtatc
     1201 caacagcacc gggttacgct actttgcgca gccttaacca gattattacc tatgatgctt
     1261 accatatggc ggaagtgtac ctgactcagc cgatgcagat tgtggcgggc agccaggcag
     1321 ggagcaaatg gatgagtgac gacctgtacg atcgggcatc aagccaggat aaacgctatc
     1381 acattgttga aggcgcaaac catatggatc tttatgacgg caaagcctat gtggctgaag
     1441 caatttcagt attagctccg ttctttgagg aaactctgta atgaaacata ttcaaatccg
     1501 caatagtgat atggcctggc atattgctgc caatattcag tttccaccaa attttgatga
     1561 gtcaaaacag tatccggcga ttatcagcgt ccatcctttt ggcagttgta aggagcagac
     1621 gtctggcaat atctatggca aggcattggc tgaaaaaggc tatgtcgttc tcgcttatga
     1681 cgccagtttc cagggagaat ctggtgggta gcctcgttgg atagaagatc cgacacagcg
     1741 cgtagaggat attagccgtg ttatcgatta tgcagtaact ctgccatatg ttgatgctga
     1801 acgcattggc gtgctgggtg tgtggggggg ggggggtggc tatgccatta atgccgcgct
     1861 gacagaaaaa cgaattaaag ctgtagtgag tattacgggt gttaatatcg ggcgtctttt
     1921 tcgggaaggt ttcagcaatt atgatccgat tggcgtattg aatgcaatgg cgtcacaacg
     1981 agcaaaagag gctcgtggtg gagaattaca gatcaacgaa ttacttcccg ccagcctgga
     2041 tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat gaagcaactg actactataa
     2101 aacaccacgc gggcaacaac cgggaggtgc gacaaaaatg ttgttctcac atgcacagaa
     2161 aacgctggcc tgggatgcct tcgcatttac tgaagtgtta ctgacccagc cggtgatggt
     2221 agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg aagtctatgg
     2281 ccgggcgatg gtttcgcagg atcgtcagtt agtttcgtta cccgatttct cacattatga
     2341 actatatgac aaaccggaag cagttcagga ggcgctggca aaagttattc cttttttcaa
     2401 cacccatctg ggataagttt catgagaaat attatgaaaa atgtactgat tcttggtgct
     2461 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac tattaaacaa
     2521 actttatttg cccgtcagcc agcaaaaata cacaaaccct atccaactaa tagccagatt
     2581 attatggggg atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggatatt
     2641 gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg
     2701 atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggtat ttatgatgaa
     2761 gttccaggaa aatttgtcga atggaataat gcagttattg gtgaacccct aaaaccattc
     2821 cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc
     2881 tggctgactg atgaagatat tattgattat gagcttacct ctcgcaatga accattcaaa
     2941 ggtacgatcg tatcacgtaa gagtgtcgca gcattaatta cagatatcat cgataaacct
     3001 gagaaacaca ttggtgagaa tataggcatc aatcaacccg ggactgatgg cgataagcca
     3061 ttcttcatgt aaatcaatta tatagatacc atcgtactgt actatgtgca gtacgattta
     3121 ttaaacttat taaggtgaac tgatcaatca gatgcgatta acatgtgtag aaccagtaag
     3181 aataaaatac gccatgtata taacattacg acaattctaa tatctgcaca tcaatgaaaa
     3241 tgcaaacaac ttccggcaaa aaagtgatac aagatcgatg acatagcgtt gaagaaaagg
     3301 atttgataat ccgcaaaatt tgcacaacac tgcctgtaat acttcctgaa ccagagacga
     3361 tcatgaccgt aacccgctca cgcgccgaac gcggcgcatt tccgcccgga acagaacatt
     3421 acggacgttc attattgggc gcgccgttaa tctggtttcc tgcccctgca gccagccgcg
     3481 aaagtggttt gattctggct ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct
     3541 cctgtgctct gcggactttg acaccttctt tgcgccgcca tcatgtggtg ctgtgtgtga
     3601 atcctgacgg ctgccagctg gggttacggg ccaatgctaa tggtgtggat ttaaacagaa
     3661 actttccggc ggcgaactgg aaggaaggtg aaacggttta tcgctggaac agcgccgctg
     3721 aagaacgtga tgtcgtatta ctgacaggtg acaaacctgg ctccgaacct gaaacccagg
     3781 cgctgtgcca gcttatacac cgcattcaac ctgcctgggt ggtctcattc cacgatccac
     3841 tggcttgtat tgaagatccc agacacagcg aattaggcga atggctggcc cagtcgtttg
     3901 aattgcctct ggtaaccagc gtcggttatg aaacgcctgg ctctttcggc agttggtgtg
     3961 cagatctcaa cttgcattgc atcaccgcag agtttccgcc catctcctcc gacgaagcca
     4021 gcgaaaaata tctctttgcg atggcaaact tgctgcgctg gcatcctaaa gatgcaattc
     4081 gcccgtcgtg aactgaagcg ccggttccac atctaccgcc agccaggtcg gtccgtcaag
     4141 atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg ctaatggcac gagaggtaca
     4201 caacatgcag cccagcatca gactgaaacc ttgtgcacgc gcttcagtcg ccagcgcttc
     4261 cgtcagacca ccggttttat cgagcttaat gttaaccatc tcatagcgcc ctttcagcgc
     4321 cttcagattg ctacgagtat gacaactttc atcagcacaa atcggcaacg gatgaataaa
     4381 attctccagc gccgcatcgt cctgcgcagg aagcggttgt tcaagcatcg caacgcctaa
     4441 atccgccaat agctggcaac gcgccgccaa cccttctgca cgccaggatt catttgcatc
     4501 aacgatcagc gtggcatcgg gcacagcggt gcgaattgcc accatccgct cactgataag
     4561 atggttatcc agcttcactt tcagtaattt cgcgcctgcc tgccagagtg ttgatgcact
     4621 attggccatc tgatcaggcg taccgatgac aaccgtctgt gcagtgataa ctgtctcggg
     4681 aagcgttatg ccgatcaaat cagccagcga ttgctgctgt tttcgcgccg cgagatccca
     4741 caatgcacaa tccagcgcat tacgtgctgc gccggcaggg agaatttttt gcaactcctc
     4801 ccgtgtcagc cctttctcta attgcggcac gacgctcata atttgcgcca ttaccgaggc
     4861 atcactttct ccataacgcg gatacggcgt gcattcgccg gtgcctttaa taccctcttc
     4921 ttccagttca accaccacca cgcgcgcttc actgcgactt ccccgggcaa tcacaaacgg
     4981 ggtatgtaag ggccaggctt cctcaaatac cttaacgatt ctcatcttta ctccttgttg
     5041 tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg tgttattgac agttagcata
     5101 aactaggtgt gacgttaact atatgtaaac aggaaagata atcatgtcac aaaccgttca
     5161 tttccagggc aacccggtta cagtcgccaa ttccatcccg caggcgggta gcaaagcgca
     5221 gacttttact ctcgtggcaa aagatctgtc tgacgtcacc ctcggtcagt ttgcgggtaa
     5281 acgcaaagtg ctgaacattt tcccgagtat tgataccggt gtttgcgccg catcagtacg
     5341 taagtttaac caactggcaa ctgagatcga caacaccgtt gtgctgtgta tctctgccga
     5401 tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct
     5461 ctccactttc cgtaacgctg aatttctgca agcctacggt gtggcaattg ctgatggccc
     5521 actgaaaggt ctggcagcgc gtgccgttgt ggttattgac gaaaatgaca atgtgatttt
     5581 cagccagctg gtggatgaaa tcaccaccga gccggattac gaagcagctc tggctgtact
     5641 gaaagcataa gttaatatgc ctgatggtgt tgcaccatca ggcatattcg cgcttactct
     5701 tcgttcttct tctgactcag accatattcc cgcaacttat tggcaatcgc ggtatgtgaa
     5761 acgccgagac gttttgccag tttgcgcgtg ctgggataat tgcgataaag ctgggttaat
     5821 accgagcgtt caaaacggct ggtgatttcg tccagcgaac cttccatcgc atcttcgccc
     5881 acggctaccg ttgcggcgtc ataatccggc aacaaaatat cctgtggacg cagctcataa
     5941 ccgtccagtt gtgtcagtgc gcgatagata gcgttcttta actgccgcac atttcccggc
     6001 cacgcataac gtgtaagtac agtattcagg tcagcggcca gtttcggacg cggcacgccc
     6061 tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg gcataatgtc ctgcggacag
     6121 tcacgtagcg gcggcagatt gagcgtcaac acgttcagac gataatagag atcttcacgg
     6181 aacacgcctt tttgcaccag ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca
     6241 tcgacatgca cctcatggtc ttcgccaacc cgacggaaag tgccatcatt aagaaaacgc
     6301 agtaatttcg cctgcatccg tggtgacatt tcccctattt catccaacag caccgaacca
     6361 ccgttcgcct gctcaaagaa ccctttcttc ccttccggag catgaccaaa cagttcactc
     6421 tcgaccgcat cttctggtat agacgcgcaa ttcagcgcca ggtaaggttt gcccgctctg
     6481 gggcttgcct gatggcaggc gtaggcaaaa agatctttac ctgtacctgt gtcacccgta
     6541 atcagcagcg gcgcgcttag catcgccagt ttctgcgcct gttcgacaac gtgcttcatt
     6601 ttcgggctga cggcgacaat ttggctgaag gcgctgacgt cctgggcggc gacattttgc
     6661 aactggcggc ccatacgaat cgttgatcgc aacatcacca ccgcaccggt caggacgtgt
     6721 tgatcatttt catcctgaag ataaacaggc gtaatctcca tcaggaaatt ctgcccatta
     6781 ataacgacat gctcgttatg cgaatcttgc ggttcgcttt ccagccaacg taaaaaatta
     6841 aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc gatccaattt ttgcccaaaa
     6901 agctgacagc tcgccgggtt cgccatatcc actttgcttt tcatatcgac agagagcaca
     6961 ggttcaggca acgcctccag taacgcgctc aacgccagat gctcacgttc ggaaggcatc
     7021 cacgggacag tacgcacatc ggtaacacct gcaatacggc gtatttcggc catcagactg
     7081 ctgaaactct caaactccag ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc
     7141 tcaataccgc gtaaatcaat gcctcttagc acgagtagat cgagtaattc gcgggtcaga
     7201 ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa ccttcacctg aaaaaagaac
     7261 aattaatatg atgatatgac actatttgga tagcaggaag gtttctgtca acaatcattg
     7321 acacaaagac ggaaaaacgg gcacttcggc gtaaagatat ccggtcagca cccgatattg
     7381 cattattttt cgctgggcgt tttgcctttt tgcagagttt ctttcacctg accgataagc
     7441 tgacgacgga aatccccaag gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa
     7501 agctccatag ctttaatccc gagtcgtacc gtcagaagtc cagcaccaat tccctgagct
     7561 gcgcgggtag acaaacgagc agcgagatct tgcgacatcc agtccatccc cacttcgcgc
     7621 accagttcgc tggctccggc aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa
     7681 cggctgtaat accccagttc aatgccatac agcgtggcaa tgcgattaat taaacgcaga
     7741 ttgcgccagg cgataaacgc catatcgacc aacgccagcg ggctgaccgc aatcatcaac
     7801 gttgattccg ccgccgaatg gctgatttcg cgccgcgcct gggcatctaa aactggctgg
     7861 accaaatgcg catacaggct gaccacttca cgatcgttct gcgtttcatg gattgaggca
     7921 taccagcgtt gcagcgccgg atgcgactga tcaatacccg cctgctgcgc cagtttttcg
     7981 caaaatgcgc gacctttgcc cgtgccgtgg ctatgcaata aatcacgcgc ttcgtcgcgt
     8041 tcatgggcgc gctgtcgcaa gcgccataag cgccgccact ctgttaccac agaacctacg
     8101 ccagcgccga taatcaatgc cccagcggca catccaccca gcgctaccca gtcctgggtt
     8161 tgccaggcat tcattgtcca ctgtataccc tgcccgacaa cgcttgcgcc aaacagagcc
     8221 agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc tgcgtccatt
     8281 accgcttcga cttgcccctc ttcttcctgc gcttcgtcga gcgtggccgg ggcaaaattt
     8341 tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg ttcgacctcc
     8401 agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat ttatctccta
     8461 ttaaaaattc cagcgcggca tcaagacgaa tatgcggcag cggtttgtcg acatccatca
     8521 cctgcgggcg aaacgcttca aactggaacc cttgcttgtc ccagaacgcc tgaccgggca
     8581 aacgcgcagg aacttcaccg gggtaaacgg tgagaggcgc accatcgctg agccggttac
     8641 cgcgcaatgc cggaattttc tcgccgttga cgtcaataat gccgctggtg gtcgcctgca
     8701 ccgaggccag ccccaggcaa tccatactaa tcctttcaaa cgccgcattt tgccaggcat
     8761 cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgacc gttacatggt
     8821 ccgctttcat cgcggcaaac agcagtttgt cgataaccgg cgagaataag cggcgaaaca
     8881 gcgtgcgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga cgcatatcat
     8941 taaatgcctg tggcccactg ttgagaggtt gcaggcaatc caccagcaca atctggcggt
     9001 caaagcgcag aaaatgattc ttatagaacc ccttcaccac tttctcgcag taataattaa
     9061 accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt ttggactcgt
     9121 cccaggcatc gacatccggc cacgggaaga attgcagcgc gggcgcacct gccatatctc
     9181 ccggcaagac aaagcgccca ggctgaataa agtgcagccc ctgctgttta cagtggtgga
     9241 gataatcggt ccacacggcg gcaatgtctg ccagccggtt ttcgtcggca ggcgctagcg
     9301 ggtcaagtcc ttcgcacatc attcgccatt tcgccgacca ttcgccgcgc tgaccattga
     9361 gtaagcccgt catctggcgc gaccagctta agtaatcctg cgccagcatc ggcaggtcga
     9421 gcaaccattc gccgggataa tccacaattt ccagatacag cgtggaggta tccttaaagt
     9481 ggcgcagcag cgaatcgttc gatttaaaac gtaacgccag gcgaatttca ctgacgccgc
     9541 gcgtaggcgt cggccaggcg ggaggatcac catataactg cgccagtcct tcatcatagg
     9601 tgaagcgcgg aatgccaaag tcacgctggg gaatgcgttt tacgcccagc aagcgctctt
     9661 cacgcaccgc gcttaacagc ggcaaacgtg ctccggcatg aatattgagc aactgattaa
     9721 ccatcgcggt gataaacgct gttttgccgc tgcggctaag tccggttaca gcgaggcgca
     9781 gatgtctgtc gacaccccga ttcaccagcg cattgagttc atttttaagt cgcttcattg
     9841 ctgtcctgtg ctgctctgaa gaccagaaaa aaaatcattc gtaaggatat gaatgtgggg
     9901 gccaggccat tgattatcaa ctgcccgtgt tgtcatcgac gggcgaactc cataagatgt
     9961 gcagcattca cgttcggacg gagttcctga cctatgtccc ctactattta tgatattgcc
    10021 agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc tgaataagca aactaatatc
    10081 tccccggaag cgcgcgaaaa agtgttacgg gccattgaag aattacagta tcagccaaac
    10141 aagctggccc gcgcgctgac ctcttcgggt tttgatgcca ttatggtgat ttctacccgt
    10201 tcgaccaaaa ctacggcggg taatccgttt ttctctgaag ttttgcatgc catcaccgcc
    10261 aaagcggaag aagaaggttt cgacgtgata ttacaaacgt cgcacaatcc ggcagaagat
    10321 ctgcaaaaat gcgaaagcaa aattaaacag aaaatgatta aaggcattat tatgctcagt
    10381 tcgccagcgg ataagtcatt ttttgcccaa ctcgataaat acgatattcc ggtggtggta
    10441 attggcaaag ttgaaggtca atatgcccat gtttattctg tcgataccga taattttggt
    10501 gacagcattg cgttgaccga tgcattaatt gaaagtgggc atcaaaatat tgcctgcctg
    10561 catgctccgc ttgatgtcca tgtttcggtt gatcgcgtta atggctataa gcaaagtctg
    10621 tccgcgcata atattgcagt gcgtgataaa tggattgttg acggcggtta tacccatgaa
    10681 acagcactgc aagcagcacg gcaattatta agtcagtcac cgttgcctga ggcagtgttt
    10741 gccactgaca gcctgaaatt aatgagcatt tatcgtgcgg cggcagagaa aaatattgct
    10801 attccgcagc agttagcggt ggtgggttat agtaatgaaa cgctgtcgtt tattttaacg
    10861 cctgcaccag gcggcatcga tgttccgacg caggagttag gtcaacaaag ctgcgagtta
    10921 ttattccgct taatttcggg aaaaccgtca ccacagaata ttaccgttgc cacgcatatg
    10981 acgctgaaat aaataccagc taccttgcct gaatgccgga tgcgatgctg gcgcatctta
    11041 tccggcctac atttatagat cggataaagt actcgcgccg catccggcaa tttagcccat
    11101 tatcagaacg agtaatttac gccgacacct gcataatgga atttatcgct gtcgccttcg
    11161 tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg aaagtccgtt ctggaaatca
    11221 taaccgtaaa ataaacccac acggttaaaa tcatggcctt cacgttcaat atcatcctgc
    11281 cagtcccagt tactctagcg atccagccca atgcgcgtat acggcgtcac cgagtggttg
    11341 ccgagcgtca gcggcaaata ggcgcgaatt tcttgtgtgg aaaactcacc gttattgcgg
    11401 ctgtcgtcca tattgaagcc gcgctcgaga taatagttca ctcgcaaagc aaccgtttcg
    11461 ttgaaggtat attgcagacc tgtttccgtt tcgacacggg tatcagcgta accggtcgtg
    11521 ttcaaatcgt tggcaaattt atacatcgac aaccaaccgt tgaaacgtaa atcatcagtc
    11581 agtttcacat cccagtctgg cgcgattttc cagcgctgca tattcgccgt gtctttgccc
    11641 ggttcatcaa cgtagtgata accataatta cggaaaccgc cggtcaggcc gaaactgaaa
    11701 tcatcgcttt cgaggaactg ataatgcacc tccagctccg ggcgatcaaa cctcgttcca
    11761 cgtttacccg cgctataatc taccggccct tcctgataat aggccagagc aattctccac
    11821 ggcccgttgg cggcattaaa atagactgaa ggctccgcca gcccatccat atcttcgcca
    11881 taaccctcga cgttttctat ttcgtacatc gcaccgatat taaagtgcca gtcgctcttt
    11941 tcctcggcct gtgcgcaggc cattcccgca cacatcacca gtgcggtaca gggtaataac
    12001 tttttttgtt ccttgtatta tcctggggga atgccttccc cctgtattta gcgaattgct
    12061 atttccgttt ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat agtgatattt
    12121 tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg cccccaaccg
    12181 tggtgtagag cataaattcc gcaccggtta attctgccac gctaattttg gcggaaatgt
    12241 tattttcctc ttgcgcgtcc ggatgaatat cttccgggcg tattcccatc acgatgggct
    12301 tatgcaaact ttcctgtgtt ttcagaaccg ctaatttctc ttcgagaatg gttaatttaa
    12361 gcgtttccgt aacgaattta tcgccatcga tcgtgccgcg aataaaattc atcgctggtg
    12421 atccaataaa tccggcaaca aacatattcg cgggttggtt ataaacggtt ttcggcgcac
    12481 ctacttgctg aacaatcccg tctttcataa tcacaatccg cgccgccatg gtcatcgctt
    12541 cggtctgatc gtgggtcacg tagatcatgg tggtgttcag tttctgatgc agcttgctga
    12601 tctctgcacg catttgtacg cgcagcttgg catcaaggtt agagagcggt tcatccatta
    12661 aaaacacgcc cgcttcgcgc acaatcgccc gcccgagcgc cacacgctgg cgttgcccgc
    12721 cggaaagcgc gcccggcttg cgtttcaggt actcacgcag gccgagaatt tgcgccgccc
    12781 agttcacccg ctcatcaatc acctctttgg cgattttttg catcttcagg ccaaacgcca
    12841 tgttgtcgta aaccgtcata tgcggataca acgcgtagtt ctggaacacc atcgcgatat
    12901 tgcgtgcttt ggctggaacg tcattcattc gtttgccgtc gatcaacaga tcgccgccgc
    12961 tgatctcctc aagcccggca atcatgcgca gggtggtcga tttaccgcag cccgacgggc
    13021 cgacaaatac gatgaactct ttatcggcaa tttccaggtt gaagtccttc accacatgca
    13081 cctggttatc gtagattttt tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt
    13141 tgctatacgt tttgccagaa ggccgataac cgcgaccagg tcagggagtc cgttgagggc
    13201 aacagtaatt gcgccccggt taagcccgca ccgatcccca ccgagcgcat accgctggcg
    13261 ttaatggcgt caatgcccgc ctgcgcatct tcaatgccga tacatgcctg cggcggcacg
    13321 cccagcccgg cacaggcagc gagaaagatt tccggatccg gtttcgagtt tttaagttgg
    13381 gaagcatccg cgcagaaggt gaaaaactcg cgcagctcca gcgccgctaa aatcgtcggc
    13441 gcattcaggg agacggaagc cagccctacc ggaatctgct gtgcacggag ctctgccagc
    13501 aaattgcgaa taccgggcag cacagcatta accgtcagct cgcgcagtga gtggacgtaa
    13561 agcagatttt tgcgatacgc cagttgcgcc cgctcctgcg ggttaaagtc gccctctttg
    13621 cccccgtgtt gcagaatgcg ccgcagagac tcatcgcggc tgatcccttt tagagattcg
    13681 ttaaactgcg catcaatact gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa
    13741 tgcagatgcg cggtatcggt gattacacca tccagatcga aaattacccc ttgcagtttc
    13801 attcatcctc ctgatgcgtg gtagcggtcc cattgaaggg caaaataaaa tcacccaaac
    13861 agaaaacaga ttcttcagcc acggatataa gctgaccgtt caaacgcagt gaaacgggcg
    13921 cagaagttcg aatcgcaata cgctgcgcgt cgagagtgac ctgtaattcg cagccctgcc
    13981 agaacagagg gaaagacaac tgttgccact gctcaagtaa cgccggattt agatgcaatt
    14041 caccgtcacg cacgctcacc ccggcaaaac cctgaatcgc ccccagccag atagcgccag
    14101 ttgcggcagc atggatacca tcatcacaac tatgcggatc agcaccaaga tcgatttcag
    14161 tcccctcgcg ccagaactga taactttggg tcagcaggcc acagcgtgca gcaacaatgc
    14221 cgtggattgc tttacttaat gacgagtcgt gaatagtgcg cggttcataa aattgcagat
    14281 tggcaagaca cgatgccgat gagaactgct ctggcagcat gtaattgagc atcaccacat
    14341 cagcttgttt gaggatctgc atctcgttca cttctgcgcg tgaataatcc agcagaatgg
    14401 tttgcttccc cgccgccgct ttgtatttcg ccagattaat cgccggctta gtcataaacg
    14461 aatcatcctg cggcaaaaca ccgtcgggct gaatttctgg cagccgcagt tctttaagga
    14521 acatttcggc acgatgaata aacgcgtcgt cactacagcc aaactggcgg gcaatactca
    14581 gcgcctgctg gacgttgtaa tacaccatat agctggtgaa ggcgttatta ttgacatgtt
    14641 cggtatattc gtctggccca ataacatcat gaatttccag acggtcgtta accctcaccg
    14701 cgcggctaat ccagaacttt gcagtttcca gaagtagcgc catgccttca tgcgcaatga
    14761 aactttcatc ccccgtggtc tgccagtatt gaataaccgc ccaggcgata tcggccacca
    14821 gatgatgttc cgcctgcgcc gaggccactt tttgccgcag cccggtgcga atgttaatgg
    14881 cggcaaattc cggcgtctct tcttcgccgc tgcgcgcgct ttcccacgga aatagcgcgc
    14941 cctgccagcc gttgcgtcgc gctttctcct gcgcgcctgg caagttgtgc cagcgataac
    15001 gcagtaaact tcgggcaacc gtcggatcgc taaacagatg gaacggcagc aaaaatactt
    15061 ctgtatccca gaaaacgtgg cctttgtagc cttcgccggt taagcctttt gccgcaatac
    15121 tgctgcgctc gccgtgagcc ggcgtcatga tgcgcagatg ataaagcgca taatctaacg
    15181 cttgctgatc gtgcgcatcg ccgccattta ccgtgatacg acgtttctgc caccattgac
    15241 gccagttttc tgttgatgct gcaagatgtt ggtcgtaact ctgttgcgcg cacatttcaa
    15301 gctggcgaag cgacacgctg ccccactcgt ctaaagcagc ttgcctgtca tcccgccagt
    15361 cgatccacac cagtttttgc aacgtcactg tctcgcctgc atgaagctgc gtactggtat
    15421 gttgcagtaa acggcgctct ttggcggtat agcattgctg cacatcaccg ctcacctggc
    15481 agcaacaact gatggccaca tcactgcgcc catcctgggt ggtgtagatc ccctgcatca
    15541 aatgctgacc aaacacccgc acctgggttt cgtcgagatg ctggcgaccg tggttggttt
    15601 gcgtggcgtc aatgcctgtt gaaatcagca ctgacgcgtc ggcgtccagt ggcataatag
    15661 tgatttccag cgcaatgagc ggcagttggt ctgccgaaac aaagcgacgg ctggcgatgg
    15721 tgtaacctgc gccgttgctg gtacgccaga caacgctacg gcgtaattct ccactggcaa
    15781 agtcaagttc acgctgccag gcttcgcggg ataacgagaa aacctcacca tttatggcaa
    15841 tctccatccc caagatatca ggcagattca ccagttcgtt gatttcacct tttcccgccc
    15901 gatgatacag ccccgccaga tacatccctc gcgtctggcg cgtgtaatct tcttcatggc
    15961 tggcgcgaag cccaagatag ccgttcccct gcgccatcag cgatgcatac ttgttcaggg
    16021 tatgctggct gaaatggggt tctgataacg ttactggcct ggtcataatt ccacacgcgt
    16081 ccctgtttct gcggattgat ataacgccgc caccagttgc tggatgatgt acccctgctc
    16141 ggcgtcggct atcatcacgg gcttgccctg tacgtgattg ataaaggctt ccatgctgcg
    16201 caaatggcgg ttgtcgtctg ccatttcccg ttgcatcagc gtcattaatt caccgttgtt
    16261 atcggtgtag atatgtgctg gaaacagcgt cgcaccggct ttatcaccac aaaagctgac
    16321 gttcataatc aactgttcgc ggatgttgag tgcaaatgac gtttccagcc acagaatgcc
    16381 gccgttatga aattcaatgg tgccaaacag cgaatcttcg acgctgtaag ttgccggatc
    16441 ccactcgcca aattgaccac agctcttttg cgtgccgatc ttttgaaaac tatgcgcatt
    16501 cacgcttttc accgccggaa agcccagcac atacatcgca gcatccagca tatgaatgcc
    16561 gatgtcgatc agcaggccac caccctgcag ttctttattg gtaaagacac cccagccggg
    16621 aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa atctctccca aaacgccgtt
    16681 ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac gatggtgaaa gtcgtaggcc
    16741 agcaccttgc ccagtttgcg cgcggtatcg cacatttccc acgcctgttc tgtcgtcatg
    16801 gcgggcggtt tttcgcacat cacatggcag cctgcttcca gtgccatcag ggtatgttcg
    16861 taatgaaaac ggttaggtga gccgacgcta accacatcag gtttcaccgc cagcagcatg
    16921 gcctgcggat cgtcccacac ggatgcattc ccgtattttt ctgccagcgc ctgcgcctgg
    16981 gaaaggcggc tgtcacagac agccaccagt tccagatcgt tgcgggtgca gtagtacgaa
    17041 gcatgaacct tatccgccac ctggcccgcg cctattatcg cgacccgcag cggagagctt
    17101 gtcattgcac ttttcacaaa gacctcttag caggtacgca accaggccaa cgaatcacgg
    17161 tacgcctggg caggatcttc tgcccggata cgcccttcat acaccacata accctgatag
    17221 ttatcggcgc gcagctgttc aaacagcgcg tggaaatcga gggtgccact gcccggctgg
    17281 taacgatggt tatcggcaat atgcacatga ccgagcaagt cacggttgtc gtgcagcgcc
    17341 tgcgccaggt tatcttcttc gatgttcatg tgatagaaat cgccgataat ctgcacatgt
    17401 ttaagattgt tttcgacgat gtaacgacga gcatcggcga gggtgttgat catatgatcc
    17461 tgatagcggt ttaacggttc gagatacacc acggttccgg tacgcgcggc gacctgttcc
    17521 agtacgcgca gggaatcact caccagtttg cggtcgccgt ccaggctacg cggcgaggtc
    17581 atcggcggta agcggaaggt aaacatgccc cacgctgccg gaaacccccc acttctgcca
    17641 gcgcctcgag aatgcgttcg atctgcttta agccattaag acgacgctct tcgataaagt
    17701 caccaatcca gccgtcatag ccgccgcagg cggtggtcac tggtaaaccg gtttctttga
    17761 tcgccgcttt gacttcttcg atgttgttga ccagcagttt gccgtcaatc tcaaaaccat
    17821 cgaagcccat ctctttgata taacgaaatc tctcgcggat attttccgga aagaatgcct
    17881 gattctgtgt gccgattttc attttttcat tcctttgaat taaaacgtaa cgcccatttt
    17941 gatgctctgt tccggatgct gatcgacata ctgcatgtag ctttccgggc tgttggcaaa
    18001 ggtcactacc gggtcgatta aatcttcgca attgagataa ccgttcatca gcagttccca
    18061 gcaggtttct tcaatacgct tacggctcca gcgcggataa tccgggttcg gttcgctgca
    18121 cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct tcgcgtccga ggttaaaacc
    18181 ttcagcaaat ggtttggcaa acgcgacata ggagatggtg ccgccgtagg ccaggccgcg
    18241 cagcgccgat tgcagcgcgt cggcgtaacc gctggtttcg ataatcacat cagccccctg
    18301 cttgccggtc agcgttttga tctctttgcc tacgtcagtg ccaatggggt taaggcagaa
    18361 atcagcaccg tggcgacggg caatatcaca gcgatgggcg ataggatcga cgccaattac
    18421 caccgatgcg ccagcgcgtt tcgccagttg gatggcaatt tgaccgatcg cgccaagccc
    18481 taccaccacg acaaaatccc ctacgcgtac gttggcatcg cgcacgccgc tcatggcaaa
    18541 ctgcgccggg tcgtaacaga cggcgttttt ccaggagctg ccttgcggca ttttgcgcag
    18601 cttgtagtta ttcactgcgt taatgatgac cgtctcggag agcgggccgt agccgcatac
    18661 gctgtcgccc accgcgtagt ctgtaacgtc gctgccgcac tcgataatgt cgccaaccac
    18721 catgttgcca agctggaatt tgccaaactc aatgccgcgc ggcgcatctg ccggacgcgg
    18781 ggtgaacatc tgccactcgc cgttaaagtc ttcatcgata aacgggctgg cggcgcggaa
    18841 gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc acgcggatct tcacttcatt
    18901 ggctaaaatc gcccgatctt catactcaac cagcgcagca acacgcggtg ctgtggctac
    18961 taactttttc atgtaaaact ccttgtaaag aaactggccg ctttagccct tcacgccacc
    19021 ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca tacataatca ccaccggcaa
    19081 tgcggtcacc agcgaggccg ccatcatccg tccccagatg taatcaggcg tactgaacag
    19141 cgcgttcagg cccactggta aggtgaaatt gctggcgctg gagaggaaaa tcgacgcaaa
    19201 cagatagtcg ttccacgcca ccataaagca gtagacgaac accgatatca gcccggacat
    19261 cgccaggggc acggtgatac ggaagatgat ttgcagacgg ttgaggccat ccatcatcgc
    19321 cgcttcttcg atctcatcag ggatggtgtc gaagtaactt ttcagcataa acacggcggt
    19381 gggcagggtt tgcgtcacca tggtaatgat cagcgccatc tcggtgtcgt aaataccaag
    19441 cgcggtgatg attttgaaca gaggcaccac cagcaaaatg ccagagaaca tatacaccgt
    19501 gtaaaagctg gcgttgatgg tcatccggcc tttaaagcgc aggcgggaaa gcgcgtaagc
    19561 tccgagaatg ccgagaaata ccgccaccac ggaggaaacc accgacacca ccatgctgtt
    19621 acggaagtag tcgacaaacg ggaaaatcat cgggttaaaa atgtcgacgt aatgctccag
    19681 cgtccactgt tgcggcagca gcgtaggatg cagcgatatc gcctctttcg cgcttttgaa
    19741 cgaggtcatc agcatcacaa aaaacggaaa cagcgtgatg atgagaaaca gcgccagccc
    19801 gcagtaaaaa ccgatacgac tgagcgtgcg tttatttgtt gccattgagg ttcacccgtt
    19861 tcctggtcag cagaatgaca gcgaaaataa tgatgaagag caccaccgag atcgccgccg
    19921 ctttccctaa gtcattaaag gcaaaggcgg ttttgtagag atagacacca agaatatcga
    19981 ccttggttgt cagcagataa acatcagcga acatgtagaa catccagatg gtgcgcaggg
    20041 tcaccaccgt cgccaggacc ggcataattg cgggcagcgt gacgatgcga aaccgctgcc
    20101 aggcattagc gccatccatc tctgccgctt cgtacagcga tttgtcgatg gtctgcaaaa
    20161 tcgccagaaa cgagataaag gcatacggga agtagcgcca gatggcgaac agcaccacca
    20221 gcacaaagct actgcccgga ttatcaaacc acagcggtgc ctgctcatag agatgcagaa
    20281 gatcgacgcc gaggtagttc acaatgccgt agccgttgtt gaacatgtat ttccaggcaa
    20341 acaccagcga aatggacggc gttacgtagg agaggatcac cagcgaacgc gcggttttgc
    20401 gcaggcggaa ctcgcggttg aaaaacatcg ccacggccag cccgagcacg gtgctgcccg
    20461 ccaccaccag cgccgtatac cagaccgtca tccacagcga gtgccagaag ccgggatcgg
    20521 agagaatgcg cacatagtta ctcaccccaa caaacgttga ctcgatgttg ggattgagcg
    20581 gcagacgtaa aaaactgatt tcgatattcg acaccatcgg ccacgccacc agaccgccca
    20641 gcagtaataa gctgggcgcg agaagcagca gcgcaaacgg catatcggaa cgacctgaaa
    20701 aaagcctgtt cattgcggat tcctgtcctg ggtttagtgc tgttcgatca gttcatccag
    20761 ctttttctgg ctcgcttgca gcgtgccagg gagatcggct ttacccacgg tgacgttatg
    20821 caccatggag ctcaccacac cagaacccgt cacatcgccc atgcgggtaa agtttttatc
    20881 ccctactgcg ccaaagacct gaatatttgg cagttcaccg attagctgat tcggtagttc
    20941 acccagcgcc ttaataacgt cgttgtcttt ccaggtggcg gtagtgacaa ccgctttatt
    21001 caccggcagc gcagcacctg gcgacatcat cacccagtcg gcaatgttgt ccgcctgctc
    21061 cataaaggtg acaaattttt ctgctgcttc ggtcttttgt ccggcggtaa tggtcagcga
    21121 ggtcaacatg ccgtagaccg cagagttttt ctcggttggc accacgaaac cgacgttttt
    21181 cgggtcgcct tctttaatca cagccggaag gatataggtg gagtaaatcg ccatcggcgc
    21241 ggtgccgttc ataaaggcgt ctttcacttc catgatgtcg ttagaacccg gcatggtgtt
    21301 ggcagcaagg tcgcggtaat aggtcagcgc ctgcatcatc tctggtgtat caagggtgat
    21361 tttgccttcg gcgttaaaga cgttggccta gttggataac gcaaactggg agaaggattg
    21421 ttccgtcagc acgctttctg ccgtaggcag cgcaataccg tattttttat tcgccgggtc
    21481 attcagtttc tgtgcaacgt ccagcagctg ttgccagttt ttcggctcct ccagccccgc
    21541 ttttgccagc acatctttgc gataccagat gtcgccaatc caggcgctga caggaacacc
    21601 ggtccatgcg ctaccatctt cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc
    21661 ttcaccaacg ttgctgatga ccgtggcaac cgctttgcga tcgataagct gctctttatc
    21721 cataactttg gcgtagtcat ggctggtttc gatcacttcc ggcagcgagc cgctacgtga
    21781 aagggtgatg actttggtgt tatgagcgtc ttcttccacg ggcacttgtt taacggtgat
    21841 acctgggttt tctttttcaa aacgggcaat caatttgctg ataactgcct gacgctcctg
    21901 ctccaccgaa gaatgcataa attcgatgga tacatgggtt ttattttctt ctttacagcc
    21961 tgaaatcagg gcgcaggaaa ccagtgctga taacagcacg atttttgatt taatcatgag
    22021 atgttccttt ttatttaatc cacataacct gccaggggcg cagcgttatt tcattaccca
    22081 atatatcaac ttcactaatc agatcccgac catgcagatc agtaatattt acatgctgaa
    22141 tatttttact gacattaaac aatccagtca gacaattacc atcagcgtta cttctttgaa
    22201 tacgcattac ggatgaatta accgtatcga tggtaaaatt attatccgga tgaaactcgt
    22261 tatggctgcg acgaagtgta attaaacgcg acaactcatg atataccgca tgccttaatg
    22321 tagcttcatc gttcagttct cgtgttatct cttcactgtg atatttttta cggttaatcg
    22381 cacggttata tccgagtttt tcgacacctg cgtaatcatt acgcgagcca agaatacttt
    22441 gaatatatat cgcgggtaca ccggggaaac ttaacaaaat cgcatgggca aggataaacc
    22501 tggtgcaacg ttcttcatcg ctactctcgc ggcggcttaa cgcattcata taggtgacat
    22561 tcatctcata gggactgcgc gtgccgtcgg gattattttt ccagtttact
//
LOCUS       DAJTNV010000062        22103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNV010000062 DAJTNV010000000
VERSION     DAJTNV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO199_002512"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OO199_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5536505.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="OO199_002513"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="OO199_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5536506.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="OO199_002514"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="OO199_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5536507.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO199_002515"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OO199_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5536508.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="OO199_002516"
     CDS             3251..5011
                     /locus_tag="OO199_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5536509.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="OO199_002517"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="OO199_002517"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5536510.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO199_002518"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OO199_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5536511.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO199_002519"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OO199_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5536512.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO199_002520"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OO199_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5536513.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO199_002521"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OO199_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5536514.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="OO199_002522"
     CDS             complement(9679..11586)
                     /locus_tag="OO199_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5536515.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO199_002523"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OO199_002523"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5536516.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="OO199_002524"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="OO199_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5536517.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO199_002525"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OO199_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5536518.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPYVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO199_002526"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OO199_002526"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5536519.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="OO199_002527"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="OO199_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="OO199_002528"
     CDS             complement(17598..18113)
                     /locus_tag="OO199_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5536520.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="OO199_002529"
     CDS             complement(18121..18663)
                     /locus_tag="OO199_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5536521.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKFT
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="OO199_002530"
     CDS             complement(18761..19744)
                     /locus_tag="OO199_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5536522.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..>22103)
                     /locus_tag="OO199_002531"
     CDS             complement(19735..>22103)
                     /locus_tag="OO199_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5536523.1"
                     /translation="LNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPAF
                     KQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINAL
                     FSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYLQ
                     RAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSNA
                     QITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVPL
                     LQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAMF
                     GLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYST
                     SGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGLF
                     YLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSLP
                     LNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGYN
                     SEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSLG
                     ETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLEN
                     AVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDG
                     SLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5478 a         5792 c         5193 g         5640 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgtag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtgaac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaa
//
LOCUS       DAJTNV010000063        21511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNV010000063 DAJTNV010000000
VERSION     DAJTNV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO199_002532"
     CDS             complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO199_002532"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5536524.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO199_002533"
     CDS             complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO199_002533"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5536525.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3125..3424)
                     /locus_tag="OO199_002534"
     CDS             complement(3125..3424)
                     /locus_tag="OO199_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5536526.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3599..4594
                     /gene="wecH"
                     /locus_tag="OO199_002535"
     CDS             3599..4594
                     /gene="wecH"
                     /locus_tag="OO199_002535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5536527.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWCGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4636..5073)
                     /locus_tag="OO199_002536"
     CDS             complement(4636..5073)
                     /locus_tag="OO199_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5536528.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5119..5460)
                     /locus_tag="OO199_002537"
     CDS             complement(5119..5460)
                     /locus_tag="OO199_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536529.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO199_002538"
     CDS             complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO199_002538"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5536530.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO199_002539"
                     /pseudo
     CDS             complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO199_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8843..9835
                     /gene="xylF"
                     /locus_tag="OO199_002540"
     CDS             8843..9835
                     /gene="xylF"
                     /locus_tag="OO199_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5536531.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9913..11454
                     /gene="xylG"
                     /locus_tag="OO199_002541"
     CDS             9913..11454
                     /gene="xylG"
                     /locus_tag="OO199_002541"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5536532.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11432..12613
                     /gene="xylH"
                     /locus_tag="OO199_002542"
     CDS             11432..12613
                     /gene="xylH"
                     /locus_tag="OO199_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5536533.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12691..13868
                     /gene="xylR"
                     /locus_tag="OO199_002543"
                     /pseudo
     CDS             12691..13868
                     /gene="xylR"
                     /locus_tag="OO199_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13976..14800)
                     /locus_tag="OO199_002544"
     CDS             complement(13976..14800)
                     /locus_tag="OO199_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5536534.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15120..17150
                     /gene="malS"
                     /locus_tag="OO199_002545"
     CDS             15120..17150
                     /gene="malS"
                     /locus_tag="OO199_002545"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5536535.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17328..18581
                     /gene="avtA"
                     /locus_tag="OO199_002546"
     CDS             17328..18581
                     /gene="avtA"
                     /locus_tag="OO199_002546"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5536536.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18732..19205)
                     /locus_tag="OO199_002547"
     CDS             complement(18732..19205)
                     /locus_tag="OO199_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5536537.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19307..20155)
                     /gene="yiaJ"
                     /locus_tag="OO199_002548"
     CDS             complement(19307..20155)
                     /gene="yiaJ"
                     /locus_tag="OO199_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5536538.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20356..21354
                     /gene="yiaK"
                     /locus_tag="OO199_002549"
     CDS             20356..21354
                     /gene="yiaK"
                     /locus_tag="OO199_002549"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5536539.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21366..>21511
                     /locus_tag="OO199_002550"
     CDS             21366..>21511
                     /locus_tag="OO199_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5536540.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5404 a         5487 c         5283 g         5337 t
ORIGIN      
        1 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
       61 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      121 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      181 ctctgccagt tcgaccagcg catcctggta acgaccttcc gcaaagtacg gctccagctt
      241 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      301 ggcattcacg cgatcgctca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      361 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      421 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      481 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      541 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cttcttcggt
      601 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      661 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      721 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      781 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
      841 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
      901 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
      961 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1021 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1081 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1141 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1201 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1261 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1321 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1381 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1441 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1501 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1561 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1621 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1681 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1741 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1801 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     1861 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     1921 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     1981 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2041 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2101 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2161 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2221 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2281 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2341 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2401 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2461 cagcgggccg tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2521 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2581 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2641 tccccaggcc cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2701 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2761 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     2821 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     2881 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     2941 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3001 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3061 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3121 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3181 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3241 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3301 cttccatatt caacgaacgc agaggatcaa cctttacccg ttgtgctttc tgcacgggtg
     3361 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3421 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3481 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3541 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3601 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3661 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3721 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3781 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     3841 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     3901 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     3961 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4021 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4081 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4141 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4201 agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg
     4261 gacattatat gaattgcagt ggtgcggaaa ttttgccgat acctggtatc tttactgtgg
     4321 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4381 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4441 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4501 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4561 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4621 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4681 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4741 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4801 catcagtaga gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     4861 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     4921 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     4981 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5041 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5101 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5161 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5221 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5281 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5341 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5401 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5461 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5521 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5581 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
     5641 tggcagaagt tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata
     5701 acgctgcgca tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag
     5761 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
     5821 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
     5881 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
     5941 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
     6001 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
     6061 attgtgtggt gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
     6121 atcagcctgt tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc
     6181 ggcccaatcc agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg
     6241 tagcgcatgg caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac
     6301 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
     6361 cacaccaact gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat
     6421 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
     6481 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
     6541 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     6601 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     6661 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     6721 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     6781 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     6841 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     6901 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     6961 cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     7021 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     7081 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     7141 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     7201 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     7261 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     7321 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     7381 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     7441 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     7501 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     7561 cagcccagtt gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg
     7621 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     7681 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     7741 tcgtaatcat attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc
     7801 tagaagccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     7861 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg
     7921 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     7981 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt
     8041 gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc
     8101 aggacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg
     8161 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     8221 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     8281 ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag
     8341 gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag
     8401 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     8461 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     8521 tcgtaatatt gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag
     8581 ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc
     8641 ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     8701 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     8761 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     8821 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     8881 acttctgctt accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga
     8941 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     9001 tctcggtgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     9061 gattgaaaac atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca
     9121 ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga
     9181 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     9241 tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg
     9301 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     9361 accttacgtt gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     9421 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     9481 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     9541 aggtttatca gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg
     9601 tattgctgcc ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac
     9661 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     9721 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     9781 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt
     9841 acgccccggc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     9901 ggtggagtcg ttatgtctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     9961 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
    10021 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
    10081 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc
    10141 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
    10201 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
    10261 gacctgatga cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct
    10321 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
    10381 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
    10441 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
    10501 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
    10561 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
    10621 atgatggtcg ggcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat
    10681 gaaatattac gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga
    10741 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
    10801 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
    10861 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcgcc
    10921 caggggattg cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
    10981 gttggtaaaa atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt
    11041 gatgacgcgg cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg
    11101 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
    11161 gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt
    11221 gatattggcg cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt
    11281 gccgttattg tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg
    11341 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
    11401 gtgatggaag ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
    11461 attggccgta ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt
    11521 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
    11581 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
    11641 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
    11701 gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc
    11761 acttaccatc attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg
    11821 ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
    11881 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
    11941 acaaattggg caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt
    12001 aatggctttt gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca
    12061 gtctccggcc tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg
    12121 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
    12181 gctgttactc ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc
    12241 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
    12301 tgccgtgttc gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg
    12361 acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg
    12421 cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg
    12481 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
    12541 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
    12601 acgccgttct tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga
    12661 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
    12721 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
    12781 caggcatcac aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
    12841 aaaatcaagg actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag
    12901 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
    12961 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
    13021 tttttgcatt taaagagaaa ggcgttaacc gctttgcttt ttatggtctt ccggaatcaa
    13081 gcggcaaacg ttgggccact gaacgcgaat atgcatttcg tcagcttgtc gccgaagaaa
    13141 agtatcgcgg agtggtttat caggggttag aaaccgcgcc agagaactgg caacacgcgc
    13201 aaaatcggct ggcagactga ctacaaacgc tgccaccgca aaccgggatt attgccgtta
    13261 ctgacgcccg agcgcggcat attctgcaag tatgtgaaca tatacatatt cccgtaccgg
    13321 aaaaattatg cgtgattggc atcgataacg aagaactgac ccgctatctg tcgcgtgtcg
    13381 ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta tcaggcggca aaactgttgc
    13441 atcgattatt agataaagaa gaaatgccgc tacagcgaat tttggtccca ccagttcgcg
    13501 tcattgaacg gcgctcaaca gattatcgct cgctgaccga tcccgccgtt attcaggcca
    13561 tgcattacat tcgtaatcac gcctgtaaag ggattaaagt ggatcaggta ctggatgcgg
    13621 tcgggatctc gcgctccaat cttgagaagc gttttaaaga agaggtgggt gaaaccatcc
    13681 atgcaatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt tcaaccacct
    13741 tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat ttctactctg
    13801 tttttaaaaa agcatatgac acaacgccaa aagagtatcg cgatgtaaat agcgaggtca
    13861 tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac agccacctga
    13921 tgcgagcaaa agccttatcc gaccgacaaa tcagataagg ctcgaggaaa tgaaattaca
    13981 tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag ttgttataac
    14041 tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca gtcacttcct
    14101 gatccgcttt acgcagctgt gcacgcgatt tacggaacga agagtaagcc gggtgcgtat
    14161 tcaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt gagtaccctt
    14221 tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg aacagattgt
    14281 tattgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca
    14341 ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg atttttcgcg
    14401 tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc gcacgctcag
    14461 caggtgacca ttggccctga tactgttttg aaatcagcca gttacgttct gcagtaatgg
    14521 ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt ttccttggtg
    14581 ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta ttgcgagaat
    14641 actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac ttttgactcg
    14701 ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg agtaacataa
    14761 gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct ggtcggattt
    14821 aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa atcatatcaa
    14881 aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt cacgagttaa
    14941 cgattcagat tggcgctaaa tcgcagaaaa tgtgggggtt atcgcaaaat tcagccgttt
    15001 tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga cttaccgctc agggatgagt
    15061 tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac tcatccacca
    15121 tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc gccagctgga
    15181 cttctccggg gttccctgcc tttagcgaac agggaacggg aacatttgtc agccacgcgc
    15241 agttgcccaa aggtacgcgt ccactcacgc taaattttga ccaacagtgc tggcagcctg
    15301 cagatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc
    15361 aatggcgatt gttcagggac ggcaaatata cgctgcaaat agacacccgc tccggtacgc
    15421 caacattgat gatttccatc cagaacgccg ccgaaccggt agcaaacctg gtccgtgaat
    15481 gcccgaaatg ggatggatta ccgctcacgc tggatgtcag cgccactttc ccggaaggag
    15541 ccgccgtacg ggattattac agccaacaaa ttgcgatagt gaagaacggt caaataacgt
    15601 tacaacccgc tgctaccagc aacggtttac tcctgctgga acgggcagaa actgacacat
    15661 ccgccccttt cgactggcat aacgccacgg tttactttgt gctgacagat cgtttcgaaa
    15721 acggcgatcc cagtaatgac cagagttacg gacgtcataa agacggtatg gcggaaattg
    15781 gcacttttca cggcggcgat ttacgcggcc tgaccaacaa actggattac ctccagcagt
    15841 tgggcgttaa tgctttatgg ataagcgccc catttgagca aattcacggc tgggtcggcg
    15901 gcggtacaaa aggcgatttc ccgcattatg cctaccacgg ttattacaca caggactgga
    15961 cgaatcttga tgccaatatg ggcaacgaag ccgatctacg gacgctggtt gatagcgcac
    16021 atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa ccacaccagc tatgccacgc
    16081 tggcggatat gcaggagtat cagtttggcg cgttatatct ttctggtgac gaagtgaaaa
    16141 aaacgctggg tgaacgctgg agcgactgga aacctgccgc cgggcaaacc tggcatagct
    16201 ttaacgatta cattaatttc agcgacaaaa caggctggga taaatggtgg ggaaaaaact
    16261 ggatcagaac ggatatcggc gattacgaca atcctggatt cgacgatctc actatgtcgc
    16321 tggccttttt gccggatatc aaaaccgaat caactaccgc ttctggtctg ccggtgttct
    16381 ataaaaacaa aacggatact cacgctaaag tcatcgaagg ctttacgcct cgcgattact
    16441 taacccactg gttaagtcag tgggtccgcg actatgggat tgatggtttt cgggtcgata
    16501 ccgccaaaca tgttgagttg cccgcctggc agcaactgaa aaccgaagcc agctccgcgc
    16561 ttcgcgaatg gaaaaaaact aaccccgaca aagcattaga tgacaaacct ttctggatga
    16621 ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg
    16681 cgatgatcaa tttcgattat caggagcagg cggcgaaagc agtcgattgt ctggcgcaga
    16741 tggatacgac ctggcagcaa atggcggaga aattgcaggg tttcaacgtg ttgagctacc
    16801 tctcgtcgca tgatacccgt ctgttccgtg aagggggcga caaagcagca gagttattac
    16861 tattagcgcc aggcgcggta caaatctttt atggcgatga atcctcgcgt ccgttcggtc
    16921 ctacaggttc tgatccgctg caaggtacac gttcggatat gaactggcag gatgttagcg
    16981 gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag ccagttccgc gcccgccatc
    17041 ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac ggctttgttc
    17101 gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtga cttttccggc
    17161 ttcccgttcg tcagtacctc gggaagccgc caaccaggat aaaatgtcag ccctaatcag
    17221 cgttgcggga taaagcaccg ctcactcttc aacagaccga tttgcacccc agcaaatgta
    17281 gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg acattctccc
    17341 tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa gatctgaacg
    17401 acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccggcg cagatcccgg
    17461 aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc aaagcgactg
    17521 atgcactgtg taactacgac ggtccacagg ggaaaacgga gctactcaca ctgcttgccg
    17581 gaatgctgcg cgagaagttg ggttgggata tcgaaccaca gaatattgca ctaacaaacg
    17641 gcagccagag cgcgtttttc tacttattta acctgtttgc cggacgccgt gccgatggtc
    17701 gggtcaaaaa agtgctgttc ccgctggcac cggaatacat tggctacgct gacgccggac
    17761 tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt
    17821 ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg
    17881 tctcccggcc gacgaatcca acaggcaatg tgattactga cgaagagttg ctgaagcttg
    17941 acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt
    18001 tcccgggtat catcttcagt gaagggcgcc cgctatggaa tccgaatatc gtactgtgca
    18061 tgagtctttc caagctgggg ctgcctggct cccgctgcgg cattatcatc gccaatgaaa
    18121 aaatcatcac tgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg
    18181 gtccggcgat gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag
    18241 tcatcaaacc gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt
    18301 taccggaaaa tcgctgcctg attcataaac cggaaggagc cattttcctc tggctatggt
    18361 ttaaggattt gcccattacg accgagcagc tctatcagcg cctgaaagca cgcggcgtgc
    18421 tgatggtgcc ggggcacaac ttcttcccgg gactggataa accgtggccg catacgcatc
    18481 aatgtatgcg catgaactac gtaccagagc cggagaaaat tgaggcgggg gtgaagattc
    18541 tggcggaaga gatagaaaga gcctgggctg aaagtcacta aaaattgcct gatgcgctac
    18601 gcttatcagg cctacatgat ttctgcaata tattgaattt ggaagaattt gtaggccgga
    18661 taaggcgttt acgccgcatc tggcataaac aaagcgcact attccggcgt tgagaaacgc
    18721 cggaaaacgt ttcaaacagg ctgctgttgt agccgatgcc gctgcacttt ctcgacatcc
    18781 atgcactgca acgcctttgt cgggcaggct tcaacacagg ccggccccgt ctcccgatgc
    18841 cagcacagat cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac ctccatcgca
    18901 ccaaacgggc aagccagcat acagctttta cagccaatgc aacgtgtttg ttcaacgaaa
    18961 atatgcccat gttcgcggct tatcgcgtca acagggcaga cattcgcgca cggtgcatct
    19021 tcacactgat gacaggctac tggcgtggtc cagctgtggt ctttaatgac acgaatacgg
    19081 gaaataaact cgtctggtga caacgcagcg caatcctgat tctcctgatg cgacactgcg
    19141 caagccactt cacaggtacg gcaaccgata catttcgtcg catccgcaat aataaaccgg
    19201 ttcatgatca ttcctttgca ctaaaacagc agagtgccag agagcgcgtc cctactgctt
    19261 tgatccaacg aaggaaatgt taaagttttg gcacttgtcc aaagcgttat gtgatcgcgc
    19321 tcaggtcatc cctgacagta aatcccagtt cattagaaat agcctgcgcg gtttcacgca
    19381 gtggtttcag gagatttttc tctcccacct gtttcagacg tgatgtcgaa agcgaaatcg
    19441 acacggcgta cggcacccgc ccatgaatat caaacaccgg aacagcaata caggagacgc
    19501 cgagttcgtt ttcttctctg tccatcgccg ctccgctttc acgaatgtgc gccagttcgt
    19561 cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa cggctggatc tcatgctgat
    19621 ggttttccca gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag atcttgccca
    19681 ttgcggaaca gtagagcggc atatgctggc caatataggc acgggttcgc agcatcccgg
    19741 ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg gagaagttaa
    19801 tggtttcacc agtggcgatg ttcagtgcct caagatgcgg agcggcgata tgaatgatat
    19861 tcagcgaaga cagcgccttc tgcccgacgg caataaattt ggtagtcagg cggtaactcc
    19921 ctgcggcggg cgcggtggtc acatagccgc aggactgtaa tccctgcaat aagcgatgga
    19981 cggtactctt atttaaacca gccagctccg aaagatgcgc caacggacaa ccgtttggat
    20041 agttgctcaa aatctcaatc agcatcaacc cgcgaaacag actctggctt ccggctggac
    20101 gctctttttc ctgcgccatc tcgttctctt tttttcccat cacttctttc cccattttgt
    20161 cgcgtcctga tggtagcgca aagtgtgccg tagttcacga tctcgacaga taatttataa
    20221 ccacttgatt tttatgtctt ttgaaattca tcaatcagat tgctttgtta aaaagtgatc
    20281 gatatatttg aaatctagtt ccgcatattg aaattttaag ccaaaaaagc gatcaaaaaa
    20341 acaaggaagc ctaggatgaa agtgacattt gagcagttaa aagcagcctt taatcgggtc
    20401 ttaatttcac gcggcgttga cagcgaaacg gctgacgcct gtgcagagat gttcgcccgc
    20461 accaccgaat ccggcgttta ttctcacggc gttaatcgtt tccctcgttt cattcaacaa
    20521 ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag cctcggcgca
    20581 attgaacagt gggatgccca gcgttcgatc ggcaacctga cggcgaaaaa gatgatggat
    20641 cgcgccattg aactggctgc cgatcacggt attggtctgg tggcacttcg taatgccaac
    20701 cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta tattggcatt
    20761 tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc
    20821 actaacccgc tgatcgtcgc cattccttcc acgccgatca ccatggtcga tatgtcgatg
    20881 tcgatgttct cttacggcat gttagaagtt aaccgcctgg caggccgtca gctcccggtc
    20941 gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat
    21001 cgccgcattt tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg
    21061 atcgctactc tcctttccga cggcgcatcg gttgccgaag tcacccagga caacagcgac
    21121 gaatacggca tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc
    21181 acccgcgatg ccaagctgca acgtatcatg gattacgtta ctaccgctga acgcgctgac
    21241 gaaaaccagg ccatccgctt acccggccac gaatttacta ccctgctggc cgaaaaccgc
    21301 cgtaacggca tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat
    21361 aagtcatgat ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg
    21421 aaaaggcgct cgattttctg cgcgctacca acttcaacgt cctggagccg ggcgttgtcg
    21481 aaatcgacgg caagaatatt tatgcgcaaa t
//
LOCUS       DAJTNV010000064        21439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNV010000064 DAJTNV010000000
VERSION     DAJTNV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..134)
                     /locus_tag="OO199_002551"
     CDS             complement(<1..134)
                     /locus_tag="OO199_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536541.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="OO199_002552"
     CDS             complement(236..1216)
                     /locus_tag="OO199_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5536542.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="OO199_002553"
     CDS             complement(1224..1934)
                     /locus_tag="OO199_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5536543.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO199_002554"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OO199_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5536544.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="OO199_002555"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="OO199_002555"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5536545.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="OO199_002556"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="OO199_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5536546.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="OO199_002557"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="OO199_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5536547.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="OO199_002558"
     CDS             8511..8621
                     /locus_tag="OO199_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5536548.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO199_002559"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OO199_002559"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5536549.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO199_002560"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OO199_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5536550.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="OO199_002561"
     CDS             11503..12135
                     /locus_tag="OO199_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5536551.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO199_002562"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OO199_002562"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5536552.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="OO199_002563"
     CDS             15667..16599
                     /locus_tag="OO199_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5536553.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="OO199_002564"
     CDS             complement(16640..18070)
                     /locus_tag="OO199_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5536554.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO199_002565"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OO199_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5536555.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO199_002566"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OO199_002566"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5536556.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="OO199_002567"
     CDS             complement(19931..20200)
                     /locus_tag="OO199_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5536557.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="OO199_002568"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="OO199_002568"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5536558.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="OO199_002569"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="OO199_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5536559.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5247 a         5383 c         5153 g         5656 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgcagc cagcgtcgca cccggcattt ttttcaccaa
    21421 cgcaaaaagg ccatcctga
//
LOCUS       DAJTNV010000065        21275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNV010000065 DAJTNV010000000
VERSION     DAJTNV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..444)
                     /locus_tag="OO199_002570"
     CDS             complement(<1..444)
                     /locus_tag="OO199_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5536560.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO199_002571"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO199_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5536561.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO199_002572"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO199_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5536562.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO199_002573"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO199_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5536563.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OO199_002574"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OO199_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5536564.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OO199_002575"
     CDS             complement(5214..6812)
                     /locus_tag="OO199_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5536565.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO199_002576"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO199_002576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5536566.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OO199_002577"
     CDS             complement(8491..9069)
                     /locus_tag="OO199_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5536567.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO199_002578"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO199_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5536568.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OO199_002579"
     CDS             10508..10687
                     /locus_tag="OO199_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5536569.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OO199_002580"
     CDS             complement(10807..11166)
                     /locus_tag="OO199_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5536570.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OO199_002581"
     CDS             complement(11528..11872)
                     /locus_tag="OO199_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5536571.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OO199_002582"
     CDS             12004..13914
                     /locus_tag="OO199_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5536572.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OO199_002583"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OO199_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5536573.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OO199_002584"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OO199_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5536574.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="OO199_002585"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="OO199_002585"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5536575.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLGQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="OO199_002586"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="OO199_002586"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5536576.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OO199_002587"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OO199_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5536577.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OO199_002588"
                     /pseudo
     CDS             complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OO199_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20409..20975)
                     /locus_tag="OO199_002589"
     CDS             complement(20409..20975)
                     /locus_tag="OO199_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5536578.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21275
                     /locus_tag="OO199_002590"
     CDS             21187..>21275
                     /locus_tag="OO199_002590"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5536579.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5267 a         5273 c         5467 g         5268 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcacc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat tgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cgctctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggga
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggaagt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gggataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgagcat agctcttgaa ttgagcattt ttctgcggta
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agaga
//
LOCUS       DAJTNV010000066        21255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNV010000066 DAJTNV010000000
VERSION     DAJTNV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..792
                     /locus_tag="OO199_002591"
     CDS             <1..792
                     /locus_tag="OO199_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5536580.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(852..2150)
                     /gene="eno"
                     /locus_tag="OO199_002592"
     CDS             complement(852..2150)
                     /gene="eno"
                     /locus_tag="OO199_002592"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5536581.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="OO199_002593"
     CDS             complement(2238..3875)
                     /gene="pyrG"
                     /locus_tag="OO199_002593"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5536582.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="OO199_002594"
     CDS             complement(4103..4894)
                     /gene="mazG"
                     /locus_tag="OO199_002594"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5536583.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4990..7224)
                     /locus_tag="OO199_002595"
     CDS             complement(4990..7224)
                     /locus_tag="OO199_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5536584.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="OO199_002596"
     CDS             complement(7272..8573)
                     /gene="rlmD"
                     /locus_tag="OO199_002596"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5536585.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8630..11386
                     /gene="barA"
                     /locus_tag="OO199_002597"
     CDS             8630..11386
                     /gene="barA"
                     /locus_tag="OO199_002597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5536586.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="OO199_002598"
     CDS             complement(11618..12958)
                     /gene="gudD"
                     /locus_tag="OO199_002598"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5536587.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12979..14319)
                     /locus_tag="OO199_002599"
     CDS             complement(12979..14319)
                     /locus_tag="OO199_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5536588.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="OO199_002600"
     CDS             complement(14321..15673)
                     /gene="gudP"
                     /locus_tag="OO199_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5536589.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16107..16556)
                     /locus_tag="OO199_002601"
     CDS             complement(16107..16556)
                     /locus_tag="OO199_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5536590.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16574..17356)
                     /gene="truC"
                     /locus_tag="OO199_002602"
     CDS             complement(16574..17356)
                     /gene="truC"
                     /locus_tag="OO199_002602"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5536591.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17356..17685)
                     /locus_tag="OO199_002603"
     CDS             complement(17356..17685)
                     /locus_tag="OO199_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5536592.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18307..18852)
                     /gene="syd"
                     /locus_tag="OO199_002604"
     CDS             complement(18307..18852)
                     /gene="syd"
                     /locus_tag="OO199_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5536593.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18920..19768
                     /gene="queF"
                     /locus_tag="OO199_002605"
     CDS             18920..19768
                     /gene="queF"
                     /locus_tag="OO199_002605"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5536594.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19880..21244
                     /gene="ppnN"
                     /locus_tag="OO199_002606"
                     /gene_synonym="ygdH"
     CDS             19880..21244
                     /gene="ppnN"
                     /locus_tag="OO199_002606"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5536595.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5227 a         5671 c         5072 g         5285 t
ORIGIN      
        1 gttgcagccc aaccaactat tgtcccacaa ttacagcaac aagttacggg tcttacgagt
       61 agcttaaact cacaagaaaa gaaagaactg acgcacaagt tagaatctat tttcaataat
      121 acccaagtgc aaattgctgt attaattgtt tccacaacca aagatgagac aattgaacaa
      181 tatgctacaa gagtttttga caattggcgt ttaggagatg ccaaacgtaa tgatgggata
      241 ctgatcgttg ttgcctggtc ggatcgcact gtccgcatcc aggtaggtta tgggctggaa
      301 gaaaaggtaa ccgatgctct ggctggagat atcatccgta gcaacatgat acccgcattt
      361 aaacaacaaa aattagctaa gggattagag ttagctataa tcgctttgaa taaccaactc
      421 acttcacaac accaatatcc ggctaatcct tcagaaagtg aatcaacgtc ttccactgat
      481 cattattact ttgctatttt ttgggtattt gcagtgatgt tcttcccttt ctggtttttt
      541 catcaaggca gtaatttttg tcgcgcatgt aaaagtggag tctgtatttc agcaatccat
      601 cttttagatt tattcctgtt ctccgataaa actttttcca ttgctgtatt ttccttcttt
      661 tttactttca ccatatttat ggtctttacc tgtttatgtg tacttcagaa aagagcatct
      721 ggtagaagtt atcattcaga caacagcggt tccgctggag gctcaagtgg cggcgcatcc
      781 ggccgctggt aactccacca taaaaaatgc cagcccgaag gctgacatta tcaaatcaga
      841 taaagtcaga cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg
      901 cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc
      961 atagaaccag ttttgatctg gcctgcagca gtaccaacag ccagatcagc gatggtagca
     1021 tcttcagttt caccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc
     1081 ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag
     1141 ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg
     1201 tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag
     1261 tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc
     1321 aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat
     1381 ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc
     1441 agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa
     1501 cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc
     1561 agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt
     1621 ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca
     1681 ccaccgttga tgatgttcat catcggaacc ggcatggagt atttgcccgg agtgccgttc
     1741 agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta
     1801 gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc
     1861 aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc
     1921 tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg
     1981 gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga
     2041 gcagctgcca tgccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta
     2101 ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc
     2161 tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaagg tacgcgttgc
     2221 cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca
     2281 aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg
     2341 caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg
     2401 gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact
     2461 tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtatagctg gcgaaccagg
     2521 ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc
     2581 ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca
     2641 accgggtact tacagtctgg cacaaattct gtagagttgg cgttctccat gttggcaaca
     2701 tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata
     2761 ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa
     2821 ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca
     2881 tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc
     2941 agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg
     3001 atggtgactt cacttaccgg attcgcttct tcgaagataa cctgttccca ttcggacaga
     3061 tttgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat
     3121 ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt
     3181 tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggtac agcgcgatct
     3241 gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc
     3301 tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc
     3361 ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatagc ttcgaggaac
     3421 ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct
     3481 tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg
     3541 gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc
     3601 gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg
     3661 tccaagtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc
     3721 atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac attgaggcca
     3781 cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg
     3841 accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa
     3901 aagacgatgg aataaccaag acgggaaagc agtatacccg aacatgacct gtgccacaaa
     3961 ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc
     4021 ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat
     4081 ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga
     4141 cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac
     4201 gctccacttc gcgaaaacga cgctcgaatt tttcgttcac tttttgcaat gcgatttctg
     4261 ctttcgtccc taaatggcga gccagattaa ccgtggcaaa cagcaggtcc cccatttcct
     4321 cctccagttt agcctggtcg acaacagcct gccgcgcctc gtacatcacc tcgtcgatct
     4381 cttcgtagac tttatcaacc accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc
     4441 aacgtttctg gattttttgc gcccgcatta aagccggtaa actacgagga atatcgtcca
     4501 gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa
     4561 gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta
     4621 atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca
     4681 tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa
     4741 aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa
     4801 tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct
     4861 gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct
     4921 gtcaggtgta ataccttcga gtaaaagata tcgctggata cggctgacgc catatccagc
     4981 tatgtaagac taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     5041 tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     5101 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     5161 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5221 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5281 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5341 tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5401 gtggtgcatc aggttaccaa caccttcaac caccacacga ccgttatctt tactgcggtt
     5461 ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc
     5521 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5581 gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg gcagcagatg
     5641 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5701 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     5761 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5821 ctgtttctgg gtgataattt caatctggtc gcccatttgc agctggtagg tgaacggcac
     5881 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5941 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     6001 gacatacacc cggtcgtcaa acacctgact acgtacttcg tcgagcattt cgccggaatc
     6061 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     6121 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6181 ctccgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt ttccacccgg
     6241 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacgtaatc
     6301 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     6361 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     6421 gaggttcttt ttctgcattt tgcgccagat gctgtagatg tgtttcggac gaccatacac
     6481 ttcagcttta acgccttcag ctttcatctc agcgcgcaga tggccgacaa actcttcgat
     6541 atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt
     6601 cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     6661 acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta cacgttcatc
     6721 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6781 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6841 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6901 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6961 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     7021 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     7081 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     7141 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7201 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7261 gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7321 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7381 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7441 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7501 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7561 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgca
     7621 ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7681 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7741 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7801 aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7861 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7921 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7981 gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct gcccagacat
     8041 gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca ttgtttgacg
     8101 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     8161 aagtagttca gacttaaacg cgcgcggcgg cgatagcccc agggaacatc ggcgatcact
     8221 tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8281 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8341 gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8401 ttatcttcag taacagcaac ttccgcgttt tcctgcggca ataatccggg gataaataac
     8461 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8521 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8581 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8641 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8701 gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8761 cggtgctagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8821 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8881 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8941 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     9001 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     9061 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgcttgagct
     9121 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcct gcgtgatgat
     9181 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9241 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9301 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9361 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9421 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc tggcgaaaaa
     9481 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9541 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9601 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9661 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9721 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9781 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9841 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9901 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9961 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
    10021 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccgggct
    10081 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
    10141 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10201 tattatcgaa gggccatcca tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc
    10261 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10321 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10381 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10441 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagttaatg ctgaaaaact
    10501 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10561 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10621 taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga aacttatcgg
    10681 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcagt
    10741 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgccggacat
    10801 ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcgac aaacgccggt
    10861 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10921 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10981 caagcctggc agcggtattt cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    11041 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    11101 tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    11161 tgaggaacag ctggctggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11221 tggcagttgc ggctatagcg gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca
    11281 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11341 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11401 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11461 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11521 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11581 aaccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11641 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11701 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11761 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    11821 tggtcaggcg ctgattacct tcctgccaga tccagtgcgt atcaatagca gtaattttac
    11881 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11941 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    12001 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    12061 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    12121 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    12181 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12241 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12301 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12361 catatttttc atacgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    12421 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12481 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12541 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12601 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12661 cgcgcccact agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12721 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12781 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12841 tattacgcgt gaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatgac
    12901 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    12961 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    13021 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt
    13081 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    13141 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13201 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13261 cacatgggta aatatcgcca gagagatatc gaaatggttg ttggaatggc agccccaggt
    13321 cagcccccag tcgtcgcaaa gctgcgccac gcggactgca ccggaaagcg tccagaagtg
    13381 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13441 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgtcgaa actccgccat
    13501 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13561 atcattcagc cctttgcata aagaaatggc ttcatcaagc agccatgcac cgttgggatc
    13621 aacggtaatc cgcgcatccg ggaagctttt cttcaatgca cgaacagtgt cgatttcttg
    13681 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13741 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13801 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13861 tatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13921 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13981 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    14041 ggctgcctga ttacctttat gtacctgctg aaccacttta ttcaggcgcg caacttcctg
    14101 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    14161 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acgatattgc gggtgaagta
    14221 tgcgttatgc gcaccaccaa tattgagcaa catgctgtca tgtccggcga ccggaatgac
    14281 tttcatatca gtaataacag gactggattg tgtcgccatc ttattgcccc gcgacaggtt
    14341 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaggg
    14401 catgaacacc aacataaatc agcgccccat taaacgagcc agtcgtgcca acgatataac
    14461 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14521 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14581 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14641 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14701 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataattcca cccagcacac
    14761 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14821 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14881 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga
    14941 tctgccccca cttcacgctg aacggcactt taactttggt gctttgctga tccatattga
    15001 tcagcgcgcc acccgcggcg atatactcca gctctttctg atttaccccc ggatgttgat
    15061 ttggctcgtg gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa
    15121 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15181 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgca
    15241 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15301 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15361 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15421 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15481 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15541 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15601 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15661 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15721 gacatcgcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15781 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15841 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15901 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg
    15961 aagaaataaa gaagagtatg aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc
    16021 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    16081 aacgacattt accgctcgct gacatatcag gacaacagcg tgccccactg ttcgacccac
    16141 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16201 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa
    16261 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16321 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16381 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    16441 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16501 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16561 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16621 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16681 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16741 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16801 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatatc
    16861 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16921 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16981 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    17041 ccaccagtgg ataatccaac accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    17101 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    17161 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17221 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17281 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17341 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17401 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17461 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17521 cagcccccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17581 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17641 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17701 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17761 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17821 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17881 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17941 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    18001 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    18061 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    18121 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18181 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18241 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    18301 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18361 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18421 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18481 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18541 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18601 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18661 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18721 agttgaaata atgcacggtg aaggaacacc gtacaattcc tcacttaacg gccagctttt
    18781 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18841 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18901 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18961 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    19021 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    19081 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    19141 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19201 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19261 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19321 tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg atgaccagga
    19381 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    19441 aaaagtagtg gaagagacgc tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19501 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19561 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19621 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg tttacgcacg
    19681 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19741 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19801 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19861 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19921 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19981 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    20041 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    20101 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    20161 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccataatac
    20221 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20281 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    20341 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct
    20401 gggcctgcgt gagctgaata tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat
    20461 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    20521 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20581 catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20641 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20701 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20761 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
    20821 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20881 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
    20941 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    21001 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    21061 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    21121 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21181 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21241 gtaatctccc ctccc
//
LOCUS       DAJTNV010000067        20714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNV010000067 DAJTNV010000000
VERSION     DAJTNV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            81..350
                     /locus_tag="OO199_002607"
     CDS             81..350
                     /locus_tag="OO199_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5536596.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO199_002608"
     CDS             344..709
                     /locus_tag="OO199_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5536597.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO199_002609"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO199_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5536598.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO199_002610"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO199_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5536599.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO199_002611"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO199_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5536600.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO199_002612"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO199_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5536601.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO199_002613"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO199_002613"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5536602.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO199_002614"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO199_002614"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5536603.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12404
                     /gene="malT"
                     /locus_tag="OO199_002615"
                     /pseudo
     CDS             9674..12404
                     /gene="malT"
                     /locus_tag="OO199_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="OO199_002616"
     CDS             complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="OO199_002616"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5536604.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="OO199_002617"
     CDS             complement(13467..14693)
                     /gene="rtcB"
                     /locus_tag="OO199_002617"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5536605.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14882..16480
                     /gene="rtcR"
                     /locus_tag="OO199_002618"
     CDS             14882..16480
                     /gene="rtcR"
                     /locus_tag="OO199_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5536606.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16462..17220)
                     /locus_tag="OO199_002619"
     CDS             complement(16462..17220)
                     /locus_tag="OO199_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5536607.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="OO199_002620"
     CDS             complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="OO199_002620"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5536608.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="OO199_002621"
     CDS             complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="OO199_002621"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5536609.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18628..20133
                     /gene="glpD"
                     /locus_tag="OO199_002622"
     CDS             18628..20133
                     /gene="glpD"
                     /locus_tag="OO199_002622"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5536610.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20402..20632)
                     /locus_tag="OO199_002623"
                     /pseudo
     CDS             complement(20402..20632)
                     /locus_tag="OO199_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4892 a         5695 c         5228 g         4899 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    10741 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    10801 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    10861 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    10921 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    10981 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    11041 atgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat caggatgaag
    11101 cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca
    11161 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    11221 tggcgctagt gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    11281 tatggagttt aattcagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    11341 gggaaacgca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa
    11401 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    11461 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    11521 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    11581 cccgtagcca gttgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    11641 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    11701 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    11761 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    11821 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    11881 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    11941 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    12001 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactt aatacgctgc
    12061 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    12121 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    12181 cagaactaat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    12241 actccggtta cagcaatgaa caaattgcgg gcgaactgga agtggcggca accaccatca
    12301 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gctgccgtgc
    12361 agcacgcgca gaaactgctg aagatgatgg gttatggggt gtgagtttag ccggataatg
    12421 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    12481 tctatcaaac caaaccgcac cggcaagaaa cgctccacca ccgcgatatt ggtcagcaga
    12541 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc
    12601 agttggtcgg cgagatattc ccccaccgcc gctgggcttg ccaggtagcg tttcacctct
    12661 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    12721 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    12781 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaaa tccccgccag tgtagcgatt
    12841 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    12901 atgttcccgc gctcgccaag ttgcaaggtg ctaaacgatg ccacgggcga gacttccgtt
    12961 gccaccacac cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    13021 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    13081 gacgggttat cggtgccgcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    13141 gcgggcagca ccgtttgcag caccagcgta caacttccgg cgctaccgat agcaaagcgg
    13201 taatcgccgc cgcgcacggt gccaggccgg aagaccagac gctgcgatcc cagctccgcg
    13261 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    13321 agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg ctggccggtt
    13381 atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcacca
    13441 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggtg
    13501 tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc
    13561 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    13621 atttggtctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    13681 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    13741 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcaccttta
    13801 cgcgtcacgt agatctcttc accaaagagc tgttcttttt gcacatagtt gtggtgacag
    13861 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    13921 aacgccgtta ccacattttc catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc
    13981 caggccacgg ctcttaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    14041 tcacgcgacg gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag
    14101 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgc
    14161 tccgactcat caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt
    14221 ttatagttgt tagtgttaag gaaacgcgga tatttttgcg ttaaccactg ataaccggcc
    14281 tccagctcag cccatttcgc atcgacgtta aaaggcgggt tttcccatgc gcctttatca
    14341 cgtttacaac gcccggtagt acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc
    14401 tccgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca
    14461 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    14521 ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    14581 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    14641 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    14701 ttctcttttc gttgtttgcg gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    14761 gcagattaat tttttaattt attgtttcta aaaagattat atctttatgt ccgtaaacgg
    14821 agatttcccg caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa
    14881 gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg
    14941 cagtcagcgc tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt
    15001 catcgatcga ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa
    15061 acgcgatatc gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca
    15121 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
    15181 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    15241 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc
    15301 aactccgcgc aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga
    15361 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga
    15421 tttcctcaaa tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    15481 cgaaaaagtg gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg
    15541 taagtcattt ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg
    15601 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt
    15661 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
    15721 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
    15781 aatgctgctg aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    15841 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    15901 aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    15961 tctacgccag cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc
    16021 cacactcact ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt
    16081 tgcaacatct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    16141 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat
    16201 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    16261 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    16321 ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca
    16381 gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    16441 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
    16501 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct
    16561 ccccatattg accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg
    16621 gcgcgagttc tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag
    16681 cgagccgtcg ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga
    16741 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc
    16801 ggcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt
    16861 ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt
    16921 gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat
    16981 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
    17041 ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
    17101 gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact
    17161 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    17221 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    17281 ctaaacccac ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    17341 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    17401 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
    17461 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca
    17521 gggcgctgat aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc
    17581 cgccgagata ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta
    17641 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    17701 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
    17761 cgatcatcat cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat
    17821 aacggcgata atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac
    17881 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    17941 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
    18001 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    18061 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    18121 gtacgccacc tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata
    18181 gaccacatcg tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg
    18241 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    18301 gtcgttggtt aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg
    18361 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    18421 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    18481 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    18541 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    18601 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    18661 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    18721 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    18781 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    18841 gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
    18901 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
    18961 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
    19021 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    19081 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    19141 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    19201 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    19261 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    19321 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
    19381 gataaacgca ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
    19441 gatgtggaat acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac
    19501 ctgctgaaag tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
    19561 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc
    19621 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    19681 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    19741 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    19801 gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
    19861 tcgctggcac gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat
    19921 gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg
    19981 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca
    20041 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    20101 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
    20161 tcaatctggc ctgagactga tgacaaacac aaaactgcct gatgcgctac gcttatcagg
    20221 cctacgtggt ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc
    20281 acgtcgcatc cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc
    20341 agggaattac cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc
    20401 ccactcgccc cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca
    20461 gaaggtaaat tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag
    20521 cccccagcat ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag
    20581 tcattaccag cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg
    20641 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
    20701 ggcgaaaaaa acgg
//
LOCUS       DAJTNV010000068        20713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNV010000068 DAJTNV010000000
VERSION     DAJTNV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO199_002624"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO199_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5536611.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO199_002625"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO199_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5536612.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO199_002626"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO199_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5536613.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKCAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO199_002627"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO199_002627"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5536614.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO199_002628"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO199_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5536615.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO199_002629"
     CDS             complement(8217..9110)
                     /locus_tag="OO199_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5536616.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO199_002630"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO199_002630"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5536617.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO199_002631"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO199_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5536618.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO199_002632"
     CDS             complement(10241..11473)
                     /locus_tag="OO199_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5536619.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO199_002633"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO199_002633"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5536620.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO199_002634"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO199_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5536621.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO199_002635"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO199_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO199_002636"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO199_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5536622.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO199_002637"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO199_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5536623.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO199_002638"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO199_002638"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5536624.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO199_002639"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OO199_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5536625.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4984 a         5725 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg catttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaatg tgc
//
LOCUS       DAJTNV010000069        20604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNV010000069 DAJTNV010000000
VERSION     DAJTNV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            88..387
                     /locus_tag="OO199_002640"
     CDS             88..387
                     /locus_tag="OO199_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5536626.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            397..921
                     /gene="ygaP"
                     /locus_tag="OO199_002641"
     CDS             397..921
                     /gene="ygaP"
                     /locus_tag="OO199_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5536627.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(968..1372)
                     /gene="stpA"
                     /locus_tag="OO199_002642"
     CDS             complement(968..1372)
                     /gene="stpA"
                     /locus_tag="OO199_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5536628.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2040..2489
                     /gene="alaE"
                     /locus_tag="OO199_002643"
     CDS             2040..2489
                     /gene="alaE"
                     /locus_tag="OO199_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5536629.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2526..2870)
                     /locus_tag="OO199_002644"
     CDS             complement(2526..2870)
                     /locus_tag="OO199_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5536630.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3022..3252
                     /locus_tag="OO199_002645"
     CDS             3022..3252
                     /locus_tag="OO199_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536631.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3599..3844
                     /gene="nrdH"
                     /locus_tag="OO199_002646"
     CDS             3599..3844
                     /gene="nrdH"
                     /locus_tag="OO199_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5536632.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3841..4251
                     /gene="nrdI"
                     /locus_tag="OO199_002647"
     CDS             3841..4251
                     /gene="nrdI"
                     /locus_tag="OO199_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5536633.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4224..6368
                     /gene="nrdE"
                     /locus_tag="OO199_002648"
     CDS             4224..6368
                     /gene="nrdE"
                     /locus_tag="OO199_002648"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5536634.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6378..7337
                     /gene="nrdF"
                     /locus_tag="OO199_002649"
     CDS             6378..7337
                     /gene="nrdF"
                     /locus_tag="OO199_002649"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5536635.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7692..8894
                     /gene="proV"
                     /locus_tag="OO199_002650"
     CDS             7692..8894
                     /gene="proV"
                     /locus_tag="OO199_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5536636.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8887..9951
                     /gene="proW"
                     /locus_tag="OO199_002651"
     CDS             8887..9951
                     /gene="proW"
                     /locus_tag="OO199_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5536637.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10009..11001
                     /gene="proX"
                     /locus_tag="OO199_002652"
     CDS             10009..11001
                     /gene="proX"
                     /locus_tag="OO199_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5536638.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11193..12372
                     /locus_tag="OO199_002653"
                     /pseudo
     CDS             11193..12372
                     /locus_tag="OO199_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12496..13233
                     /gene="ygaZ"
                     /locus_tag="OO199_002654"
     CDS             12496..13233
                     /gene="ygaZ"
                     /locus_tag="OO199_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5536639.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13223..13558
                     /gene="ygaH"
                     /locus_tag="OO199_002655"
     CDS             13223..13558
                     /gene="ygaH"
                     /locus_tag="OO199_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5536640.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13649..14179
                     /gene="emrR"
                     /locus_tag="OO199_002656"
     CDS             13649..14179
                     /gene="emrR"
                     /locus_tag="OO199_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5536641.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14306..15478
                     /gene="emrA"
                     /locus_tag="OO199_002657"
                     /pseudo
     CDS             14306..15478
                     /gene="emrA"
                     /locus_tag="OO199_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15495..17033
                     /gene="emrB"
                     /locus_tag="OO199_002658"
     CDS             15495..17033
                     /gene="emrB"
                     /locus_tag="OO199_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5536642.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17097..17612)
                     /gene="luxS"
                     /locus_tag="OO199_002659"
     CDS             complement(17097..17612)
                     /gene="luxS"
                     /locus_tag="OO199_002659"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5536643.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17762..19318)
                     /gene="gshA"
                     /locus_tag="OO199_002660"
     CDS             complement(17762..19318)
                     /gene="gshA"
                     /locus_tag="OO199_002660"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5536644.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19391..19819)
                     /locus_tag="OO199_002661"
     CDS             complement(19391..19819)
                     /locus_tag="OO199_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5536645.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19816..20382)
                     /gene="yqaB"
                     /locus_tag="OO199_002662"
     CDS             complement(19816..20382)
                     /gene="yqaB"
                     /locus_tag="OO199_002662"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5536646.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4967 a         5273 c         5149 g         5215 t
ORIGIN      
        1 cttttactct catttacgct gctgaggctg gtatatgttg catttttagt ttagagttta
       61 cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca ggccagtacc
      121 gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct gattctgtgc
      181 atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac cggactgagc
      241 gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat caacagccaa
      301 cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc cattatcgcc
      361 accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac catttcgccg
      421 catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat
      481 gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt gctggaacag
      541 tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca ggcaggtaaa
      601 cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga aatattttta
      661 ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt aaataaatct
      721 caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat attaatcggc
      781 gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt tgttggtgcc
      841 gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt agataagatg
      901 ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg acatccggcc
      961 tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc tgagcaattg
     1021 gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca tcagtgaatt
     1081 tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca gcagagctat
     1141 tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc caggtgctaa
     1201 ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca cgtctttctt
     1261 tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag aattcgcgag
     1321 ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac ataataaaga
     1381 aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg atcacttatt
     1441 atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca gctgttattt
     1501 tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga gtaatttagg
     1561 ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga caaaaccgaa
     1621 gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt gctttctgct
     1681 gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg acaaacaccg
     1741 aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa attaaaggca
     1801 agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa aatccacaat
     1861 gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa ataaaaattt
     1921 ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca gatggcaatg
     1981 agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg agcattagca
     2041 tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg atggttgttt
     2101 actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc ttcgaacagt
     2161 ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg ccatacggta
     2221 tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc tggataaaaa
     2281 atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg gcgatcttgt
     2341 tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac atcgttgttt
     2401 cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga ctgtttaaag
     2461 tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca tcgccagtcg
     2521 cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc aacacgcgaa
     2581 ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata gagcggaaat
     2641 tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt tgaactgcgt
     2701 tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc gcgattaaca
     2761 tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac atcgacagta
     2821 aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat atttgatcct
     2881 cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc tttcagaaaa
     2941 gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg aaaattacat
     3001 cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt tgatgatggc
     3061 gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc tgtattgaag
     3121 tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc tcaggcgttg
     3181 ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc cgcgcgcgat
     3241 gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt gggtacagca
     3301 gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta gtgcaaaaat
     3361 gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt ttgcctggtg
     3421 aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg atcactattt
     3481 gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg tatcaacaga
     3541 gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa tacgaatcat
     3601 gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca aacgggcgat
     3661 ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg aagcagcgga
     3721 agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg atcttagctg
     3781 gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg cggccagtgc
     3841 atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt tatcgaacgt
     3901 ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca ggtagacgag
     3961 ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc ggtgccacga
     4021 caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg cgttattgct
     4081 tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat tgcccggaaa
     4141 tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga tatcgaaaac
     4201 gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg acgcaggaaa
     4261 cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca ggtcgcattc
     4321 agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg cgtccgaaca
     4381 gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa ggttactatg
     4441 atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt gcccacgcac
     4501 acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac accagctata
     4561 cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga gtcacgatgg
     4621 tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc gatgaaatgc
     4681 tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag cagcagcgcg
     4741 gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg attggtcggg
     4801 cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt ttgctgtcga
     4861 atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct ggcgtaattc
     4921 cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc gcgcgtcagg
     4981 gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt ctcgacacga
     5041 aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc gtggtgatcc
     5101 cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt tcgccctatg
     5161 acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa cactatgacg
     5221 aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat ttcttccagc
     5281 gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa gacacagtaa
     5341 accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca gaaattttgc
     5401 aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca ggccatgata
     5461 tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc gatttttccc
     5521 gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt catatccgca
     5581 gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg gggcagatga
     5641 atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa gccctggatt
     5701 tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg atgttgctgg
     5761 cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc agtggtgaat
     5821 atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt ggcgaactgt
     5881 ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg cgcgacgacg
     5941 tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc gggtctatct
     6001 cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta gagatacgca
     6061 aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac gaaaatctgg
     6121 cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc tacgcggaag
     6181 cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat accgccacca
     6241 ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa acgctctatt
     6301 acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc gtctcctgtg
     6361 cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg aacaagatat
     6421 ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg ctaccagaaa
     6481 aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta gaacaacaac
     6541 tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat gttatcggcg
     6601 cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta tcgaatatca
     6661 gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg ctgtgccaga
     6721 ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg cagcgaaaag
     6781 ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa atcgccagtg
     6841 tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat ttttccagcc
     6901 gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat gaagcagtcc
     6961 acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct ctgggacaac
     7021 gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac aacgagttgc
     7081 aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa gcgtttctct
     7141 gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt cccgcagaaa
     7201 tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat gaaaatcacg
     7261 atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa acagaagatg
     7321 aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag cagggaaata
     7381 attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca ctgatactct
     7441 gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct atgacaaaaa
     7501 atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc ggcgatttgc
     7561 tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct cagtatgtta
     7621 gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa ataaaggaat
     7681 cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata tttggtgagc
     7741 atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa
     7801 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
     7861 ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc
     7921 tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc aaaatatccg
     7981 acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
     8041 taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg gccggaatta
     8101 atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt
     8161 atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc
     8221 gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg gcgctcgatc
     8281 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
     8341 ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac cgaattgcca
     8401 ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc aataatccgg
     8461 cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg ttcagtgcga
     8521 aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc ttcggcccac
     8581 gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt atcgaacgcg
     8641 gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta acgcagcagc
     8701 aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta
     8761 gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc
     8821 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
     8881 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgcg
     8941 caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg tggtgctgac
     9001 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
     9061 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt tgttacccat
     9121 ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag
     9181 caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct catcacctgg
     9241 cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc catcggcgca
     9301 atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc cctgctgttc
     9361 tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg agcggcgaaa
     9421 attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca
     9481 atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgcg
     9541 ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt
     9601 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
     9661 ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
     9721 gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc
     9781 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
     9841 atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc
     9901 tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta actctgcact
     9961 tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat gcgacatagc
    10021 gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc tgccgatctg
    10081 ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga aaccttccag
    10141 acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa acccagcgaa
    10201 gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt caccgccgtg
    10261 aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa gaaattttat
    10321 cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa gaaaaccgcc
    10381 gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc caaactgttc
    10441 gataccaacg gcgacggtaa agcggattta accggttgta accctggctg gggctgcgaa
    10501 ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac tcataatcag
    10561 gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg caaaccggtg
    10621 ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg gaaagatgtg
    10681 gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga taccaaactg
    10741 ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc caacaaagcc
    10801 tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt gccagtggcc
    10861 gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg cgatattcag
    10921 ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg ggtgaatgag
    10981 gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg cctcatccgc
    11041 cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta caaaccacat
    11101 cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta ttattgattt
    11161 ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat gagcttagcc
    11221 cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt aactattacg
    11281 cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt tcggcaggct
    11341 ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt gttcccctcg
    11401 gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca gcgggcggta
    11461 tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg gcattaaccg
    11521 gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg gcttcaccgg
    11581 ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg atattgctgg
    11641 cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc ttttgggttg
    11701 cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca caaatgaaat
    11761 cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg ttcatcagcg
    11821 ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac ttcagcattc
    11881 tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc gatggtgaca
    11941 ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg gcgggcggtt
    12001 ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt actttcatgg
    12061 ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat tctggtgctg
    12121 gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg aatacatcct
    12181 gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgcc
    12241 ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct
    12301 ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa
    12361 gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga
    12421 agcccatcag tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg
    12481 ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc
    12541 atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg
    12601 ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc
    12661 atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt
    12721 ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt gtatggcccg
    12781 tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct atgggcgttt
    12841 ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa taatcgccgc
    12901 tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt
    12961 acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagct
    13021 gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc
    13081 aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt aacgctattt
    13141 tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag
    13201 gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg
    13261 cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc
    13321 aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc
    13381 tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac
    13441 gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc
    13501 aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc
    13561 cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat
    13621 cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat
    13681 gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg
    13741 tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca
    13801 ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag
    13861 tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat
    13921 tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata acgatcgccg
    13981 ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc
    14041 gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct
    14101 cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt
    14161 tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa
    14221 atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg
    14281 agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca
    14341 accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat
    14401 aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac
    14461 cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt
    14521 gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc tggtcactct
    14581 cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt
    14641 tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca
    14701 aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc
    14761 caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc
    14821 gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa
    14881 actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctagctggc
    14941 gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc gtgccgtgca
    15001 accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat
    15061 gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg gtcagccggt
    15121 caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag tggttggtct
    15181 ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga ccggtaactg
    15241 gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac agctggagca
    15301 atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg
    15361 tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg cgcgtgaaat
    15421 cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc
    15481 cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga
    15541 cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt gctaacgtgg
    15601 cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg tgggtaatca
    15661 cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg
    15721 tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcttgggcgt
    15781 gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg
    15841 ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac
    15901 gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccga
    15961 tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc
    16021 cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt gaaacccgca
    16081 ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc
    16141 tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag gaaattatca
    16201 ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg
    16261 ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt
    16321 gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg cagttgttgc
    16381 aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta
    16441 ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc
    16501 tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg
    16561 aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg
    16621 tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg
    16681 cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacgtcga
    16741 taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg
    16801 taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg cttgggatga
    16861 cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg ctgattattt
    16921 ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg gggctggtgt
    16981 ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac taagtacaac
    17041 taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag
    17101 atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct gttgatgcgt
    17161 acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg
    17221 taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc
    17281 acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc
    17341 agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca
    17401 ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct
    17461 ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg
    17521 tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta
    17581 tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa
    17641 attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat
    17701 catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt tttcttttct
    17761 gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt tccatttcct
    17821 gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca
    17881 gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct gttccgccaa
    17941 taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga
    18001 agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca
    18061 gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc agtaacggga
    18121 actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcaaggatca
    18181 cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc ggtgcatcag
    18241 ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa
    18301 tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta
    18361 acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcat
    18421 acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta ccgtctttct
    18481 caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta
    18541 cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc
    18601 cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt
    18661 tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg
    18721 caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat
    18781 agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc
    18841 agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc
    18901 cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc
    18961 cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc
    19021 acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca
    19081 gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat
    19141 cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg
    19201 ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct
    19261 ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat
    19321 tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc
    19381 aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat
    19441 agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag atgcgcatcc
    19501 atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga
    19561 ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca
    19621 atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg
    19681 ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga
    19741 cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa
    19801 gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg ctgcacgggc
    19861 cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat
    19921 acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcagc
    19981 agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg cctcagcgat
    20041 ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac
    20101 ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt
    20161 ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc
    20221 aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt actgaagacc
    20281 gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggattgt
    20341 gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga
    20401 aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga
    20461 aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt
    20521 tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt
    20581 actctatcca gctgagctac ggat
//
LOCUS       DAJTNV010000070        20570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNV010000070 DAJTNV010000000
VERSION     DAJTNV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            409..1239
                     /locus_tag="OO199_002663"
     CDS             409..1239
                     /locus_tag="OO199_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5536647.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO199_002664"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO199_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5536648.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO199_002665"
     CDS             complement(2368..3828)
                     /locus_tag="OO199_002665"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5536649.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO199_002666"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO199_002666"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5536650.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO199_002667"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO199_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5536651.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO199_002668"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO199_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5536652.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO199_002669"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO199_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5536653.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO199_002670"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO199_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5536654.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO199_002671"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO199_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5536655.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO199_002672"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO199_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="OO199_002673"
     CDS             complement(12470..13009)
                     /locus_tag="OO199_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5536656.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO199_002674"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO199_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5536657.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="OO199_002675"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="OO199_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5536658.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="OO199_002676"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="OO199_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="OO199_002677"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="OO199_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5536659.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="OO199_002678"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="OO199_002678"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5536660.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="OO199_002679"
     CDS             19140..20120
                     /locus_tag="OO199_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5536661.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5477 a         4908 c         4672 g         5513 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta aatttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgattcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgacatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAJTNV010000071        20507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNV010000071 DAJTNV010000000
VERSION     DAJTNV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO199_002680"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO199_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5536662.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO199_002681"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO199_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5536663.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO199_002682"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO199_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5536664.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO199_002683"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO199_002683"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5536665.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO199_002684"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO199_002684"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5536666.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO199_002685"
     CDS             4449..4814
                     /locus_tag="OO199_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5536667.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO199_002686"
     CDS             4964..5482
                     /locus_tag="OO199_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5536668.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO199_002687"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO199_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO199_002688"
     CDS             6713..7195
                     /locus_tag="OO199_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5536669.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO199_002689"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO199_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5536670.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO199_002690"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO199_002690"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5536671.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO199_002691"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO199_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5536672.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO199_002692"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO199_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5536673.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO199_002693"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO199_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5536674.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO199_002694"
     CDS             10939..11730
                     /locus_tag="OO199_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5536675.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO199_002695"
     CDS             11751..13037
                     /locus_tag="OO199_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5536676.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO199_002696"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO199_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5536677.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO199_002697"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO199_002697"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5536678.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO199_002698"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO199_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5536679.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO199_002699"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO199_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5536680.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO199_002700"
     CDS             complement(16985..17275)
                     /locus_tag="OO199_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5536681.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO199_002701"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO199_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5536682.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO199_002702"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO199_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5536683.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO199_002703"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO199_002703"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO199_002704"
     CDS             19137..20378
                     /locus_tag="OO199_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5536684.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRHAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5353 c         4809 g         5045 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaattg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcatg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattct
//
LOCUS       DAJTNV010000072        20342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNV010000072 DAJTNV010000000
VERSION     DAJTNV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO199_002705"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO199_002705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5536685.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO199_002706"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO199_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5536686.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO199_002707"
     CDS             complement(2649..3200)
                     /locus_tag="OO199_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5536687.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO199_002708"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO199_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5536688.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OO199_002709"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OO199_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5536689.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO199_002710"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO199_002710"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5536690.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO199_002711"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO199_002711"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5536691.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO199_002712"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO199_002712"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5536692.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO199_002713"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO199_002713"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5536693.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO199_002714"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO199_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5536694.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO199_002715"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO199_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5536695.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO199_002716"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO199_002716"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5536696.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO199_002717"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO199_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5536697.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO199_002718"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO199_002718"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5536698.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO199_002719"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO199_002719"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5536699.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO199_002720"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO199_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5536700.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO199_002721"
     CDS             complement(18174..18569)
                     /locus_tag="OO199_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5536701.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO199_002722"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO199_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5536702.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO199_002723"
     CDS             19485..20078
                     /locus_tag="OO199_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5536703.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..>20342
                     /gene="tabA"
                     /locus_tag="OO199_002724"
     CDS             20223..>20342
                     /gene="tabA"
                     /locus_tag="OO199_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5536704.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIE"
BASE COUNT         4834 a         5130 c         5392 g         4986 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctagcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aa
//
LOCUS       DAJTNV010000073        20277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNV010000073 DAJTNV010000000
VERSION     DAJTNV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO199_002725"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO199_002725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5536705.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO199_002726"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO199_002726"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5536706.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO199_002727"
     CDS             complement(1728..2999)
                     /locus_tag="OO199_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536707.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OO199_002728"
     CDS             complement(3182..4387)
                     /locus_tag="OO199_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536708.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OO199_002729"
     CDS             complement(4487..4843)
                     /locus_tag="OO199_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5536709.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO199_002730"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO199_002730"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO199_002731"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO199_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5536710.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO199_002732"
     CDS             6773..9829
                     /locus_tag="OO199_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5536711.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO199_002733"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO199_002733"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5536712.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO199_002734"
     CDS             10336..10524
                     /locus_tag="OO199_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5536713.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO199_002735"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO199_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5536714.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO199_002736"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO199_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5536715.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO199_002737"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO199_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5536716.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO199_002738"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO199_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5536717.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO199_002739"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO199_002739"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5536718.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO199_002740"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO199_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5536719.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO199_002741"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO199_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5536720.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO199_002742"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO199_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5536721.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO199_002743"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO199_002743"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5536722.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5311 a         4856 c         5174 g         4936 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacaccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa actgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gtgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa ggtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gactgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagcctg gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga ttccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca taacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattga
//
LOCUS       DAJTNV010000074        20217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNV010000074 DAJTNV010000000
VERSION     DAJTNV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            11..277
                     /gene="mliC"
                     /locus_tag="OO199_002744"
                     /pseudo
     CDS             11..277
                     /gene="mliC"
                     /locus_tag="OO199_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            336..992
                     /gene="pdxH"
                     /locus_tag="OO199_002745"
     CDS             336..992
                     /gene="pdxH"
                     /locus_tag="OO199_002745"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5536723.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1121..2395
                     /gene="tyrS"
                     /locus_tag="OO199_002746"
     CDS             1121..2395
                     /gene="tyrS"
                     /locus_tag="OO199_002746"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5536724.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNRGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2454..3317
                     /gene="pdxY"
                     /locus_tag="OO199_002747"
     CDS             2454..3317
                     /gene="pdxY"
                     /locus_tag="OO199_002747"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5536725.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3361..3966)
                     /gene="gstA"
                     /locus_tag="OO199_002748"
     CDS             complement(3361..3966)
                     /gene="gstA"
                     /locus_tag="OO199_002748"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5536726.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4072..5574)
                     /gene="dtpA"
                     /locus_tag="OO199_002749"
     CDS             complement(4072..5574)
                     /gene="dtpA"
                     /locus_tag="OO199_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5536727.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6185..6820)
                     /gene="nth"
                     /locus_tag="OO199_002750"
     CDS             complement(6185..6820)
                     /gene="nth"
                     /locus_tag="OO199_002750"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5536728.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6820..7515)
                     /gene="rsxE"
                     /locus_tag="OO199_002751"
     CDS             complement(6820..7515)
                     /gene="rsxE"
                     /locus_tag="OO199_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5536729.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7519..8139)
                     /gene="rsxG"
                     /locus_tag="OO199_002752"
     CDS             complement(7519..8139)
                     /gene="rsxG"
                     /locus_tag="OO199_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5536730.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8143..9201)
                     /gene="rsxD"
                     /locus_tag="OO199_002753"
     CDS             complement(8143..9201)
                     /gene="rsxD"
                     /locus_tag="OO199_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5536731.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9202..11424)
                     /gene="rsxC"
                     /locus_tag="OO199_002754"
     CDS             complement(9202..11424)
                     /gene="rsxC"
                     /locus_tag="OO199_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5536732.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11417..11995)
                     /gene="rsxB"
                     /locus_tag="OO199_002755"
     CDS             complement(11417..11995)
                     /gene="rsxB"
                     /locus_tag="OO199_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5536733.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11995..12576)
                     /gene="rsxA"
                     /locus_tag="OO199_002756"
     CDS             complement(11995..12576)
                     /gene="rsxA"
                     /locus_tag="OO199_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5536734.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12653..13093)
                     /locus_tag="OO199_002757"
     CDS             complement(12653..13093)
                     /locus_tag="OO199_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5536735.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13179..13394)
                     /gene="ydgT"
                     /locus_tag="OO199_002758"
     CDS             complement(13179..13394)
                     /gene="ydgT"
                     /locus_tag="OO199_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5536736.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13667..13792)
                     /gene="blr"
                     /locus_tag="OO199_002759"
     CDS             complement(13667..13792)
                     /gene="blr"
                     /locus_tag="OO199_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5536737.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14035..15075
                     /locus_tag="OO199_002760"
     CDS             14035..15075
                     /locus_tag="OO199_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5536738.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15111..16112)
                     /gene="add"
                     /locus_tag="OO199_002761"
     CDS             complement(15111..16112)
                     /gene="add"
                     /locus_tag="OO199_002761"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5536739.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16216..17388)
                     /gene="malY"
                     /locus_tag="OO199_002762"
     CDS             complement(16216..17388)
                     /gene="malY"
                     /locus_tag="OO199_002762"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5536740.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17398..18990)
                     /gene="malX"
                     /locus_tag="OO199_002763"
     CDS             complement(17398..18990)
                     /gene="malX"
                     /locus_tag="OO199_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5536741.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLPHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19165..20193
                     /gene="malI"
                     /locus_tag="OO199_002764"
     CDS             19165..20193
                     /gene="malI"
                     /locus_tag="OO199_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5536742.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5140 a         5119 c         5201 g         4757 t
ORIGIN      
        1 aatatagggg caaatccagg ttgagcgaat gcaaaccgat acgctggaat accagtgtga
       61 tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaagtcagtt ttgtttacga
      121 taatcaacta ctgcatctca aacagggcct ttcagcctct ggcgcgcgtt actccgacgg
      181 gatctatgtt ttctggtcga aaggtgagga agcgactgtc tataaacgcg accgcatcgt
      241 cttgaataac tgtcagttac aaaatccaca gcgttgagat ttttccaggg gcggcgcaca
      301 atagcgtcac ccactgacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc
      361 aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatcttcccg
      421 ccgatccatt aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg
      481 accctaccgc gatggtggtc gctaccgtgg atgaacatgg tcagccttat cagcgcatcg
      541 ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc ggtagccgta
      601 aagcacatca aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg
      661 aacgccaggt gatggtgatc ggtaaagcag aacgactttc gactctcgaa gtgatgaaat
      721 attttcatag ccgcccgcgt gatagccaga ttggtgcatg ggtttcgaag cagtccagtc
      781 gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac
      841 agggcgaagt gccattgccg agcttttggg gcggttttcg cgtcagcctt gaacagattg
      901 agttctggca gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg
      961 atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc
     1021 tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg
     1081 taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat
     1141 taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga
     1201 gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt
     1261 gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa
     1321 gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc
     1381 cgagcgtaag ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa tccgtaagca
     1441 ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta
     1501 tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc
     1561 cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg
     1621 gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa
     1681 caaacagtac gatgtggtgc tgcaaattgg tggttctgac cagtggggta acatcacttc
     1741 tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct
     1801 gatcaccaaa gcagacggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga
     1861 tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga
     1921 cgtttaccgc ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctaga
     1981 agaagaagat aaaaacagag gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt
     2041 gactcgtctg gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct
     2101 gttcagcggt tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg
     2161 cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga
     2221 actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa
     2281 cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg
     2341 ttttacctta ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat
     2401 taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga
     2461 aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag
     2521 agtttccgat gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta
     2581 atcacaccca atacggcaaa tggactggct gcgtgatgcc gcccagccat ttaaccgaaa
     2641 ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct
     2701 atctgggatc ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag
     2761 cgaatccgca ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa aaaggctgta
     2821 tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca
     2881 ttgcgccgaa tctggttgag ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag
     2941 aagcggttct ggcagcgcgc gaactcattg agcaagggcc acaaattgtg ttggttaaac
     3001 acctggcgcg agctggctac agccgtgacc gttttgaaat gctgctggtc accgccgatg
     3061 aagtctggca tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg
     3121 gtgatgtgac gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg
     3181 ccctggaaca tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg
     3241 aatatgagct gcaagtggtg gctgctcagg atcgtattgc caaaccagaa cattacttca
     3301 gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gacctgtggg ccgatggcta
     3361 ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg
     3421 cataaatgcc gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg
     3481 cagaaccgta aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc
     3541 atccttcagt gcctcgttca catattgcag cttcttatcc agctgcgcgc gaactgtcgg
     3601 tttgtactct tccggtgtat ctgggcgaaa cagaggtgtg aaacctttat gcagctcggt
     3661 ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag
     3721 caactggcgg tcggggacgc tgtcggcaag atactgcata atcgcaacgc cttccgtcag
     3781 caaagtacca tcgtccagca gcaatgcagg cacctgcccc ttagggttaa cggaaaagta
     3841 atcgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt
     3901 tccgctctca cgcagggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa
     3961 tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa
     4021 aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac
     4081 agtagctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttaggcgc
     4141 ggtcagcagc atcagtactg caataacggc agtagcgaca ccaatctgca agaatactcg
     4201 accatagact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag ccatcatacc
     4261 cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat
     4321 gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca gaccagagat
     4381 catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctga cagacacgat
     4441 acctgcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat
     4501 caccatgccg attgcaaact tggttggcat cggcagggta tcacccatct tgttatagat
     4561 agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct gatactgctc
     4621 aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa agaagttcag
     4681 tgacgttggc atctggctgt acagcacgaa gaagatgatt gcttcgagca tcaggatgaa
     4741 ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaagcttctt taccgaagat
     4801 aaccacaata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag
     4861 tagccaggtg gcaacagcga tcagcgccac aacaccaata atggtcagca acaggttacg
     4921 gtagttgact ggttcaaagt ctggttttga accgtactgt ttaacccagc gttggcagaa
     4981 ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca
     5041 gccgtatttc gcggccagcc acggggtggc tatcatagag aagaaagagc cgatgttgac
     5101 ggacatgtag tacatggtga atgcaccgtc cagacgtggg tcgtttttct catagcatgt
     5161 agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat
     5221 acccatataa acaataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc
     5281 cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca
     5341 gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga
     5401 atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc
     5461 gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg
     5521 tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac
     5581 ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata
     5641 cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca
     5701 gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg
     5761 gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt
     5821 gagaggggga tgatggcttg gcggcgatat gttctgctat ttagcgtttt tcctagtgag
     5881 atagtccagt ttctgaaaaa tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg
     5941 tgtaaattta aatcatttta acctgattct tgcaatgttt ttacattgcc acagcatcgt
     6001 gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt
     6061 tggttatccg caaacggatt acctgatgat aacgctaagc aatggcatca ttgacgttaa
     6121 aaagatgttt taaaagagga taaagaaagg ttatcaatgg ggtaatcggt gttacccctt
     6181 ttcttcagat gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac
     6241 agcggggctt gcgggcaatg caggtataac gcccgtgcag gatcaaccaa tggtggcagt
     6301 cgactttaaa ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt
     6361 tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag
     6421 tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct acgccgggca
     6481 gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgttgttcc agtaatatac
     6541 ggcaggtttt gatgatattt tctgctttgc tgttataaag gccaatcgtt ttgatatagg
     6601 ttttcacccc ttcaacgcct agttcaagca tcgctgcagg cgtattcgcc accgggtaga
     6661 gtttcgccgt cgccttatta acactgatat cggtcgcctg agcggaaagc agtacggcaa
     6721 tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac
     6781 gcaggcgagt gaggatctcc agacgttttg ctttgttcat cagacattcc ctgtttcacc
     6841 gtttggcaat gcacgttcgg cagctgcttc agcacggcgc tttttcattc tttcatcaat
     6901 caggtatttt cctgccagca tcagtcccaa gccaataaat gcacctggtg gcagcatcgc
     6961 cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg cccagctacc
     7021 taacagcgca tctgcaccgt caaacaacgt gccattgccg ataatttcgc gtagtgaacc
     7081 cagcacgaac atggcgcagg ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag
     7141 cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac
     7201 aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg cgttgatcag
     7261 catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc
     7321 ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt
     7381 agtcgcaagt cctaaaccca gagcgttagt ggcagtggat gtgaccgcca atagcggaca
     7441 aaggccgagc agttggacca gtgcagagtt gtttttccac aacccctgaa caataacgtc
     7501 tttaatttcg ctcacgcttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa
     7561 cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt
     7621 cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt gcgcatcatc
     7681 cgcaccactg attttttttc ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat
     7741 tttatcgcca agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc
     7801 attaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc
     7861 ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc
     7921 tttacctaac tctggcgcag ttaccagata gcaactctgt gcgagcgcat tgttatagcg
     7981 ttcggctggc agcacctgat caaataacgt cttttgttgc agactggcct gttcagcaat
     8041 tgtcgttttg gtcatctggt tgatggccgc agttaaccct gtagaacccg ctgcaaatag
     8101 tgccagtgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc
     8161 gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac
     8221 ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataagcc
     8281 cgccagcgcg ccgaacatca gccgaccacg attggtcgta gaagcagtaa ctgggtcagt
     8341 caaaataaag aatgcgccga gcatagtcgc accagacagc agatgaattt gcggtgctgc
     8401 cagtgtttct ggtgagaaca accagcccaa cgttgcgcat aacgccagcg ttactaagaa
     8461 gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccacacgc caccagccag
     8521 ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt agatcggata
     8581 ttgcataatc tgttcaaccg aatgacctgc gcggacagag gttttgaatg tatccagcgg
     8641 tgtcgcctga ctaatgccat caatacctaa gcgtagcgta ttcatatcac caccactggc
     8701 ggtatgaccg ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat
     8761 ttcatgtggc ggtaaccagc tggtcatctg caccgggaag gagatcagta agaccacata
     8821 accaatcatt gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat
     8881 gatcaccgca aacaccgtac ccagtacgac catccaccat ggcgctaggg ggggaatact
     8941 taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg cggctaccga
     9001 ctgcttgcgt agtttgagta cgagagcttc ggctaacaga gcactgaccg acgccaacag
     9061 gatctgaacg agagtacccc aaccaaaaaa ccacagttgc gctgcgattc ctggcacggc
     9121 tgcgagcaac accaacagca taatgcgcga tgtctggcgc tggttatggg tataagggga
     9181 gctagctatt ctgaatacca tttagtcctc gtttacaacc ttttgctggg cggctttttt
     9241 ggcctgaacg cgggcaatag ccgcggcaac tgcggctttg cgcggatcga cctgttcttc
     9301 tggttccgca ttagcctgtt gctgttccag cttgcgtgct ttggcgcggg caatagcggc
     9361 ttcgacggca gctttgcgcg gatcaacctg ttcttctggc tccgcattgg cctgttgctg
     9421 ttccagcttg cgtgctttgg cgcgggcaat agccgcttcg acggcagctt tgcgcggatc
     9481 aacctgttct tctggctccg cattggcctg ttgctgttcc agcttgcgtg ctttggcgcg
     9541 ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttcttctg gttccgcatt
     9601 agcctgttgc tgttccagct tgcgcgcttt ggcacgggca atagcggctt cgacggcggc
     9661 tttgcgcgga tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg
     9721 cgctttggag cgggcgatag ctgcttcaac ggcagtttta cgtggttcag caacggttgc
     9781 tgcgtcgtta gtttgctcca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
     9841 ccgttctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
     9901 tgtagcctgg gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc
     9961 tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagtcg ctttttcgcg
    10021 ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc
    10081 ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca agggaatatt
    10141 gctcgggcaa acccacgcgc aagccccaca ttcaatgcag tcggcaatgt tatgcgtggt
    10201 agctttatcg tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccac
    10261 agggcaggcg tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc
    10321 aagttcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag
    10381 ccatggcaag gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg
    10441 gcagaatccg gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt
    10501 gcccgggcga gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc
    10561 atcaataacg gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat
    10621 atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt
    10681 agcaccgcca gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc
    10741 cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat
    10801 ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca
    10861 atcctgcatc aaacggtcat cggcggtaat gtacggctcg cactcagcag cgttgataat
    10921 caacgtttca atcttatctc caccaccctg caatttaacg cctgtcggga atccggcacc
    10981 gcccagcccg gcaacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgaga
    11041 gcgataatcg acccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat
    11101 cacgcttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac
    11161 ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg
    11221 gccgcgcaat actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg
    11281 tttcagtgga ataacaaaat gctgcgccag tagtacctgg cgcagggggg taccgttggt
    11341 ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct
    11401 gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt
    11461 tcagatccca tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg
    11521 tcgggcacgg atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca
    11581 tgacacgggt agcgccaacg atggcgtcta ccggacaagc ctgaatacat ttagtgcagc
    11641 caatacagtt attttcatca ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt
    11701 cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt
    11761 cgccacctgg ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg
    11821 ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt
    11881 tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc
    11941 caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa
    12001 cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc
    12061 aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc
    12121 ggcgaagagc accatcacca gcgagaaacc gacagcggcg gaaaaaccgt acagcgccga
    12181 ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca ctgcacagtt
    12241 ggtggtgata agcggcaaaa aaatccccag cagacggtaa agcaccgggc tggttttgcg
    12301 caccaccatc tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg
    12361 caggtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga
    12421 cgtcagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt
    12481 ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag
    12541 tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc
    12601 gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat
    12661 tcacaaacgt cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg
    12721 ccaacaaagt aaacagcaac tggcgaacgg ctatgccaac ttccacgggt gaaaaagcga
    12781 atgcttttac tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac
    12841 aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag
    12901 cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt
    12961 gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca
    13021 acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg
    13081 ttgtggtggt cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg
    13141 cgttattata aggcttttat aaccgatcgc tactttcttt attggacata gtgccagacg
    13201 gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg
    13261 tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga
    13321 tggtcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag
    13381 tcctgaacag tcataaaagg tccataaaat aattaatacc aaaggggtag gtattattag
    13441 tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt acaataataa gaatgcgctg
    13501 atggcaaata ttcagggatg aaaaactgct ctgaaaagag taacgcgctt tactatccct
    13561 gacaatcacc aacaacatcg aacaagataa taaattcctg gtttaatatc cgacaagtga
    13621 aaacatgcac ccggacgggc agcatgtcgc tccacaagtg cagagcttac ttgtgttgta
    13681 ccgaagcact ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa
    13741 tgactgaaac gacaagaacg atccaacctg ttaattcaat aagacgattc attacagccc
    13801 acatctctct tgattgatcc attaacttca gggggtaaat gttacttagc aataatagct
    13861 ccagcaagat ttttactgag gtttttgcga tatttagctt ttgtcgttgg aaaattcgct
    13921 atttttttga cttaagttaa acaacatccc ttattgctgg caggttatta aactgttgag
    13981 cgtgggtaaa ggatagtgtc aaatagccat catacttcaa tgagaggcaa tgacatgagc
    14041 gacaacatcc gtgttgggtt gattgggtat ggttatgcga gcaaaacctt ccatgcgccc
    14101 ctgattgcgg gcacgcccgg gctggaactg gcggtaatct ccagcagtga tgaaacaaaa
    14161 gtaaaagccg actggccaac ggttacggtt gtctctgagc cgaagcatct gtttaacgat
    14221 cccaacatag acctgattgt cattcctaca cccaacgata cccatttccc gttagccaaa
    14281 gcggcgcttg aggcgggtaa atatgtggtc gttgataaac cctttaccgt gacactgtca
    14341 caagcgcgag agctggatgc gctggcaaaa agcctggggc gtgtgctgtc tgtattccat
    14401 aaccgtcgtt gggatagcga tttcttgacg ctaaaaggtt tgctcgcgga aggcgtgctg
    14461 ggtgaagttg cttactttga gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt
    14521 tggcgtgaac agggcggccc aggcagcggt atctggtacg atttagcacc gcatcttctt
    14581 gatcaggcca ttacgctatt tggtttaccg gtcagcatga cggtagattt ggcacagtta
    14641 cggcccggag cgcagtcgac cgattatttc cacgccatct tgtcctatcc acagcggcga
    14701 gtcattttac acggtaccat gctggcagcc gctgagtcag cacggtatat cgtgcatgga
    14761 tcccgaggca gttatgtgaa atatggcctc gatccacagg aagaacgtct gaaaaatggc
    14821 gagcgtctgc cgcaggaaga ctggggctac gatatgcgtg atggcgtact tacccgcgtg
    14881 gaaggtgagg aacgtgtcga agaaacgctg ttgacggtgc ctgggaatta tccggcttac
    14941 tatgcggcga ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc ggcaagccag
    15001 gcaatccagg taatggagtt gattgagctg ggcatcgaat ccgccaaaca tcgcgcgacg
    15061 ttgtgccttg cgtgaaaaat ggatggtgca atttgcacca tccatttttg ttacttcgcg
    15121 gcgacttttt ctcgcagtgc gcgtttttcc tctgcgctga ggaaagccat ttccagacca
    15181 ttaatctgtg cctggcggat ttgctcacgg gataacccag cagctggcgc ggcaacggta
    15241 tattcgtgaa tgatatccac tccctgtacg ccgggatcgt cagtgttaat gctggcacga
    15301 atgccatgct caaggaacgt tttcagcgga tgtgcagcca gctctgctac ggtgctggtc
    15361 tgaatattgg aggtcagaca ggattcaata ccaatttgtt gctcggcgag aaaatccatc
    15421 agcgcccgat cttcaatggc ttttacgcca tgtccaatac gctccgcacc cagttcacga
    15481 atcgcctgcc agatgctttc cggcccggca gcttcgcctg catggacggt aatatgccag
    15541 cccgcatcac gcgcgcggtt gaagtgagaa aggaacagac ttcccgggaa tccaagttca
    15601 tcaccagcta aatcaagtgc ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct
    15661 tgctgacagg cggcttcgcc gaaggttcgg ctcataatgc cgataagctt cgcctgcaca
    15721 ccaaaggtgc ggcaaccttc acgtacgcca tcgatcaccg cttcgacaac acccgctaca
    15781 ggcagcttat gtgccattgc catgtagcct ggtgaaaaac gcagctcgac atagcgcagg
    15841 ccgttacggg ctgcatcttc aatgttttca aatgccacgc ggcggcaggc atcaagagag
    15901 gcgagaactt taacgcccca gtcaagtttg gtcagaaagc tcaccagatc gggttcgttg
    15961 gcaatgacct gaacgtgggg aatcagtgtt tcaagggatt gtgcaggaag cgagatatta
    16021 tactggcggc caagttcaag aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg
    16081 atatcagtta atggcagggt ggtatcaatc atggtcgcac tctttttctg gatataaaag
    16141 tgcgcaccat tataaaaaca aagcagggta aaaatccacg attgcgcaac gattttttac
    16201 gttgcgcaat tggggttagc gaacagcgcg gatggcgttt attaatccag ccacaccttt
    16261 ttccagtttc gaacgtgggc agccggcatt gagacggaca aaaccacgac cttcttcacc
    16321 ataggtatac cccggcatga tcgcgacttt ttcttgctcg ataagcgctt tttgcaacgc
    16381 gttgtcgtca atattcaacg gacgtagatc aagccaggcc agataagtgg attgcgggat
    16441 ctgccagttg agttcaggaa acgcggcgtt cattttatct gagatatacg tcaggttatc
    16501 tttcagatag acgcgtaagg catccagcca cggtgcacct tgctgatagg cggcgatatg
    16561 ggcagttaac gccagtaccg aaggggaaga aagcccatca cgtcctttca gtgccgataa
    16621 ataggcatca cggctgctgc tattttctat aatcccgtaa gcaccggtca gcgcgggaat
    16681 attgaaactt ttcgatcccg atgtcagcaa cgcccagtct ccccgcgcca cattactcca
    16741 gggaatatgc ggctgctcgc cccagaccat atccatatgg atttcatcgg aaataacccg
    16801 cacatcatga cgctcgcaca ggtcagccat gatctccagc tcatcgcacg tccacacttt
    16861 cccggtagga ttctgtgggt tacacaggag cataatttta cattctggtt tcgccaaaac
    16921 ggcttccagc ttgcccatat cgcaaaacca gccgtcagcc tgcttctcta aagcaacggg
    16981 cattactgtg cgctggttac cttcaatggc cttgtaaaat gcgtcatagg cgggcgtgtg
    17041 gatcaccacg ccttcacctg tttcagacca ctgacgaatc agttctgaaa ccatatagat
    17101 gacagaaggg ccatacacca ccgtctgcgt atcaattgcg gtgtaatgct gggtggaaaa
    17161 ccagtgggca atagccgcga gaaactcatc gtttttccag cggctgtagc caaatacgcc
    17221 gtgcatcagg cgctgattca gcgcctcgat aatgcagggg gcagtggcaa aatccatgtc
    17281 tgaaatcgtg aacggtaaca ggtcagcagt gccgaaacgg tcagcgacat aatcccactg
    17341 tgtacaccat gtgccatgac gatccacgac ctttgaaaaa tcgaacatat cttatcctta
    17401 tgcctggaca gtatgcatca gaccggccat ttcatctttt actgactgaa cttgtggccc
    17461 gataacaacc tgcaggttat gttgattaag ttgtactacg ccaattgccc gattgtcctt
    17521 cagtgcctgc acattaacaa gcgacatatc ttccacagac aaacgcagac gggtaatgca
    17581 gttatcgagg ctgacaatat tgtcggcacc gcctaatgct tcgaggattg caggaacgtt
    17641 gtaacctgat ttacccggcg caccggcaac ggctttttcg attgagctgg caacttcgct
    17701 atcgcgcccc ggggttttca gattgaagcg ggtgatagcg aaacggaaga tgacgtagta
    17761 aacgacaaac cagattgccg ccaccactgg caccatgtac cacttggttg acagaccatg
    17821 caaaataccg aacaccacga agtcgatgat attgccgtcg gtattaccga tggtgacgcc
    17881 gagcacagac atgacggtga agccgaggcc ggttaacagc gcgtggatga catacagaac
    17941 tggcgctacg aacaggaaca ggaattccag cggttcggta gtgccgccaa cgacgcaggc
    18001 gatcaggcca gaaatcagca gacctttaat tttatggcga ttttccgggc gcgcgcagtg
    18061 atacatagct aacgctgcac ctggtagacc gccgagaaac gcaggcattt taccttgcga
    18121 aaggaaacgc gtgacgcttt cagaaaaacc gtgagtggtc gggcaactca attgcgcctg
    18181 gaagatggtc agtgcgccgc tgacggtttg accgcagacc tcctgcgtgc cgcctgcgtc
    18241 ggtaaagcga attaatgcca ccagaatgtg aggcagacca aacggcaaca gcagacgttc
    18301 accggtacca aacagcatcg gtccgaaatc acccgcgctg tttatcatat ggcccaagcc
    18361 gctaataccc atggcgaaaa tcggccagac taatggaatc accaggccga caaggcccat
    18421 caccagcgag gagataattg gtacgaagcg cgtaccgccg aagaatgcca gcgcatccgg
    18481 caggcggata ttatggaaac gctcatgcag catccagacg ataataccgg cgatcaccgc
    18541 accgaggatc ccggtatcga tcgactggat cccaagaatg ctctggatgt tattggcttt
    18601 cagaacggcg gcatctgtgg ttggcagaat gcctttattg gtcaaccaga agtttaccgc
    18661 gaggttcatt accgcataac cgacgaagcc agcgaatgcc gctacgcctt tattttcgcg
    18721 tgccaggccc agcgggatgg cgatacagaa catgacaggc aggaaactaa aagcaaacga
    18781 gccaatctta ctcatccagg taaagatagc ctgcaacacg gggttgccca ggaccgggat
    18841 cagggttatg acatcatggc tgctaagaga actaccaatg ccgagcataa tgccgcagaa
    18901 cgacaataat gccacgggta acatgaaggt tttgcctaac tgctggaaga actcccacag
    18961 cgtgactttc ggtgctgttt tcgccgtcat aaaacgactc ctcgtagaga acaatctgga
    19021 ttcagtaaat tgcgcgagaa gataaaacgt tttatcaaat tttagtgagt cataaatcac
    19081 attacgcaac gataatagcg ggtataagat aaataaaagg taaaacgttt tatctgtcac
    19141 ataatcaggg agtaggtcat ctgcatggct accgccaaaa aaataaccat tcatgatgtt
    19201 gcgctggctg cgggcgtgtc ggtaagtacc gtttcgctgg tgcttaatgg caaagggcga
    19261 atctctaccg ccacaggaga acgcgttaac gccgccattg aagagctggg atttgtgcgc
    19321 aatcgacagg cgtcggcgct gcgcggcggg caaagcggcg tcattggttt gatcgtccgt
    19381 gatttatctg cgccgtttta cgccgaattg acggccggat tgacggaagc tctggaagca
    19441 cagggacgga tggttttttt gcttcacggc ggtaaagacg gcgagcagct ggcacagcgg
    19501 ttttcactgt tactgaatca gggtgtcgat ggtgtggtaa ttgccggggc tgcaggaagc
    19561 agcgatgacc tgcggcggat ggcagaagaa aaagctatcc cggtgatttt cgcttcccgt
    19621 gccagttatc ttgatgatgt tgatacggtt cgcccggaca acatgcaggc tgcacagttg
    19681 ttgacggagc atctcattcg caatgggcat cagcggatcg cctggctggg agggcaaagt
    19741 tcctcattaa cccgtgcaga acgggtgggg ggctattgtg caactctact aaaatttggc
    19801 ctgccgtttc acagcgattg ggtgttggag tgcacttcca gccagaagca agccgcggaa
    19861 gctatcacgg cgcttttacg tcataacccg accatcagtg ccgtggtttg ctataacgaa
    19921 actattgcga tgggggcatg gtttggtttg ctgaaagcag ggcggcaaag cggggaaagc
    19981 ggagtcgatc gttactttga gcaacaggtt tcgctggcgg catttaccga tgcgacacca
    20041 accacacttg atgatatacc tgttacctgg gccagcacgc cagcgcggga acttggtatc
    20101 acacttgcgg atcgcatgat gcaaaaaatc acccatgaag agacgcattc acgcaatctt
    20161 attattcccg cccggctcat tgcggcgaaa taatcctctc tttatctgct atacttg
//
LOCUS       DAJTNV010000075        20196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNV010000075 DAJTNV010000000
VERSION     DAJTNV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..294
                     /locus_tag="OO199_002765"
     CDS             <1..294
                     /locus_tag="OO199_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5536743.1"
                     /translation="RWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTL
                     DSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            266..2194
                     /locus_tag="OO199_002766"
     CDS             266..2194
                     /locus_tag="OO199_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5536744.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2178..2381
                     /locus_tag="OO199_002767"
     CDS             2178..2381
                     /locus_tag="OO199_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536745.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2378..3971
                     /locus_tag="OO199_002768"
                     /pseudo
     CDS             2378..3971
                     /locus_tag="OO199_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3961..5466
                     /locus_tag="OO199_002769"
     CDS             3961..5466
                     /locus_tag="OO199_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5536746.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5503..5850
                     /locus_tag="OO199_002770"
     CDS             5503..5850
                     /locus_tag="OO199_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5536747.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5908..6936
                     /locus_tag="OO199_002771"
     CDS             5908..6936
                     /locus_tag="OO199_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5536748.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6988..7374
                     /locus_tag="OO199_002772"
     CDS             6988..7374
                     /locus_tag="OO199_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5536749.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7386..7763
                     /locus_tag="OO199_002773"
     CDS             7386..7763
                     /locus_tag="OO199_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536750.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7750..8334
                     /locus_tag="OO199_002774"
     CDS             7750..8334
                     /locus_tag="OO199_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536751.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8331..8732
                     /locus_tag="OO199_002775"
     CDS             8331..8732
                     /locus_tag="OO199_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536752.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8743..9483
                     /locus_tag="OO199_002776"
     CDS             8743..9483
                     /locus_tag="OO199_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5536753.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9542..9928
                     /locus_tag="OO199_002777"
     CDS             9542..9928
                     /locus_tag="OO199_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5536754.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9937..10266
                     /locus_tag="OO199_002778"
     CDS             9937..10266
                     /locus_tag="OO199_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5536755.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10238..12976
                     /locus_tag="OO199_002779"
     CDS             10238..12976
                     /locus_tag="OO199_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5536756.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12976..13305
                     /locus_tag="OO199_002780"
     CDS             12976..13305
                     /locus_tag="OO199_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536757.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13305..14003
                     /locus_tag="OO199_002781"
     CDS             13305..14003
                     /locus_tag="OO199_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5536758.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14008..14751
                     /locus_tag="OO199_002782"
     CDS             14008..14751
                     /locus_tag="OO199_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536759.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYMLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14688..15290
                     /locus_tag="OO199_002783"
     CDS             14688..15290
                     /locus_tag="OO199_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5536760.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15351..18831
                     /locus_tag="OO199_002784"
                     /pseudo
     CDS             15351..18831
                     /locus_tag="OO199_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            18899..19498
                     /locus_tag="OO199_002785"
     CDS             18899..19498
                     /locus_tag="OO199_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5536761.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19562..>20196
                     /locus_tag="OO199_002786"
     CDS             19562..>20196
                     /locus_tag="OO199_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5536762.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         4721 a         4771 c         6374 g         4330 t
ORIGIN      
        1 agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca ggtgtcaaaa
       61 ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc gatggcttta
      121 tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga tttaacgccg
      181 cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg cgcgcgtgcg
      241 ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga gtaatatgat
      301 ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga cggctgtaga
      361 atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg gggaatggaa
      421 aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga ttcgtacagt
      481 taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg tgatcgggta
      541 ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact cagctgctga
      601 agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc tgaaaaaact
      661 ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc gtttttcctc
      721 tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg aaaaatctgt
      781 cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa aagaaggttc
      841 gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat ccatacgcgg
      901 ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg aatctgcgca
      961 tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc tgaagtttgg
     1021 cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa cggtgtatta
     1081 cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga ccggggggcg
     1141 ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt tcagtgcttc
     1201 gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg cgtacagtcc
     1261 gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag atcccaacgg
     1321 cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
     1381 actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg tgcctgtccg
     1441 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1501 ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca tgggacgtcc
     1561 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat accgccatgc
     1621 agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga tcgatggcga
     1681 aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg taaaaggtgc
     1741 ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc ggggtgtgta
     1801 tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa tgaaggccga
     1861 tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata atccggagat
     1921 tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa agtgggaaaa
     1981 aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     2041 ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt
     2101 gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac tggcagcgaa
     2161 gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc
     2221 ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc aggatcgaat
     2281 atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag tcggttctgg
     2341 gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc ctgtcctgat
     2401 tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg gcgcaggatt
     2461 tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg ccctgttgcc
     2521 tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg gtatcgcggc
     2581 taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta tcagctaccg
     2641 tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg tcggggaggt
     2701 ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg tggaagggaa
     2761 acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta acggcgaaat
     2821 ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc gttttaaagc
     2881 cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact ttctgcgggc
     2941 cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg aggatgattt
     3001 tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca ccgggcgtcc
     3061 ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg ccaatcagtt
     3121 ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc agcttcagtc
     3181 ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata ccgaaaaggc
     3241 atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta ttaatattct
     3301 ggagaagttc acctgctggt atgacacgaa taacgtgacg ctgggcggtg ttaaaattcc
     3361 gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca gacaatggat
     3421 tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc gtttcctacg
     3481 aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc tccgccaatg
     3541 agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg gccacgcaga
     3601 tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg ccacgggcgc
     3661 gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt ggctccggaa
     3721 gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt gaggccggac
     3781 tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac attttccgcc
     3841 agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg gtgtggatag
     3901 cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag acaacatcac
     3961 gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct gcttctggag
     4021 cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc agcaagcctt
     4081 tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga aacggacgag
     4141 tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg tattgctgta
     4201 ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt ttccggaatg
     4261 actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac tcaggtacgg
     4321 ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt tgactgcgct
     4381 gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa tgacacggcc
     4441 tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac ccagacatcc
     4501 cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca tctggcacag
     4561 gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg caatcagttt
     4621 gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc ggcgcgccgg
     4681 atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt tatgggaaca
     4741 gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga tgaattaatc
     4801 aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga tacaggaggc
     4861 actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg agtgatgggg
     4921 atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct ggcagggcaa
     4981 cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc gcaacaatct
     5041 ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc gaaaggacgt
     5101 gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa agcccgtccg
     5161 attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca gattatggcc
     5221 ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg tccggggatg
     5281 accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga accggtttct
     5341 gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc agcggtacag
     5401 ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct catggccatg
     5461 ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac gaaaaccatc
     5521 actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca taccgccaca
     5581 ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct ggatgaggat
     5641 accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg catactggta
     5701 ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac ctttgcgacg
     5761 gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc ctttgccggc
     5821 agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg gtagcctgac
     5881 tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca gttactcggt
     5941 tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt ccgccgtacg
     6001 gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac gccggtggcg
     6061 gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg tgaaactcgt
     6121 gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca ggcggtggag
     6181 cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg cctgcgtatc
     6241 atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga gatgcaggcg
     6301 gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga aactgttgag
     6361 gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac ggagtggagc
     6421 gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg tgattttgct
     6481 tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct gaatggcttt
     6541 aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct ggaaacggca
     6601 gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct ggcaatcgtg
     6661 gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct gccggacggc
     6721 acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc cattcaggat
     6781 gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca ctggctgacc
     6841 gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat ggtgctgccg
     6901 gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag ctaaatgctc
     6961 ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct gaaacgtctt
     7021 tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc ggcagatatt
     7081 gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt cgcagagccg
     7141 gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc agttcctgaa
     7201 cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc actggcgaca
     7261 ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc gtttcgtgtt
     7321 tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca gtaacaggag
     7381 atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc aatgaagtca
     7441 ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag gggacacacc
     7501 tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg atccggcttg
     7561 aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta cgccgtcagg
     7621 atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg gatgacgggg
     7681 gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat gccagaatga
     7741 ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct cgatacccgc
     7801 atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat tgtttctgtc
     7861 gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa aggtattccc
     7921 ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg aaaaatgtat
     7981 gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac cgcacaggtc
     8041 agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt actgaaagtg
     8101 gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg ctggcatgtg
     8161 atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat cccgatgtcc
     8221 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
     8281 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
     8341 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
     8401 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
     8461 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
     8521 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
     8581 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
     8641 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
     8701 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
     8761 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
     8821 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
     8881 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
     8941 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
     9001 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
     9061 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
     9121 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
     9181 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     9241 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     9301 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca gactgcttcg
     9361 gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatc
     9421 gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt cagcgcacca
     9481 taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc cggagaacat
     9541 gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     9601 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     9661 tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc gtatgggaat
     9721 ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt ctcaggatgt
     9781 tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg gcgcgggggc
     9841 ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg atgatgatca
     9901 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     9961 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc cactgagctt
    10021 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
    10081 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga tgctgcggtg
    10141 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
    10201 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
    10261 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
    10321 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
    10381 gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg caacagcagc
    10441 atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca tggcccgtga
    10501 ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg aggaacaggc
    10561 gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat
    10621 ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag cccggcggga
    10681 gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga ggggatccgg
    10741 gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga aaggcatgat
    10801 gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta aagcctggca
    10861 tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg gacattatgc
    10921 cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga atggtatcac
    10981 gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat ttcatggaaa
    11041 cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg gccagtcggt
    11101 cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc
    11161 gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac gcgtccttgg
    11221 ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg cagaggaaac
    11281 cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta cgctgcaaac
    11341 cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc gtgaagactc
    11401 gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa aatatccgtg
    11461 gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc ttcaggagaa
    11521 aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt accaggagca
    11581 acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa cggaagctgc
    11641 acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc aggctgtcag
    11701 ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga aaaatacacg
    11761 ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc agcaggcaca
    11821 ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg aaaggatgac
    11881 tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg acgggaaaaa
    11941 gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc aggcgctgac
    12001 gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg acctgagaaa
    12061 aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc gtgagcagca
    12121 caatctggat attgccactg caggtatggg ggataagcag cggcagcgct accaggcaca
    12181 gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg acagtcgcca
    12241 gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga aggggagcct
    12301 gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg cgcagggcga
    12361 ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg ataatgcggc
    12421 gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg cactggcgac
    12481 attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt tgtcagatat
    12541 ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg gttctttatt
    12601 cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga gtcgctacag
    12661 tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg
    12721 ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta agctgggggt
    12781 tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca acattgagat
    12841 caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg tttatgacct
    12901 cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg gccggttaag
    12961 tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat gaatgtggta
    13021 tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg
    13081 ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa aggtgaacac
    13141 ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca
    13201 ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg
    13261 ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag gatatccggc
    13321 aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa
    13381 tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag aacgaaaaag
    13441 gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag gggtcggggt
    13501 ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct aacctgcacg
    13561 gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg gtggtccggc
    13621 gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agcgacgccg
    13681 atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg
    13741 tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt ttcccggggc
    13801 gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg
    13861 gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag gacaaatgca
    13921 gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc ggtttccttt
    13981 ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat tctggcgcac
    14041 gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc ggagggagac
    14101 aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg gatggctccg
    14161 gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca cagtcatccc
    14221 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
    14281 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca tctcaccggg
    14341 cggcgctttg agcacggggt gacggactgt tacatgctgt tccgggatgc ttatcatctg
    14401 gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca cggtcagaat
    14461 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa
    14521 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
    14581 tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg agagaggtat
    14641 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg gcacgcatct
    14701 gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
    14761 ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg
    14821 gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc
    14881 ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg gtgggagcta
    14941 aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt gctatatgga
    15001 tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc agtatgacgc
    15061 ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg
    15121 ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc cagggcaatg
    15181 ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct caggagatca
    15241 gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt
    15301 ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag
    15361 gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc acgcagttgc
    15421 tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat ggattaaaaa
    15481 gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac ttttccggtg
    15541 ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg tttgaatctt
    15601 ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc acccggacca
    15661 tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca ctggtggaaa
    15721 ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag atacagcgta
    15781 acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact tcgcagtatc
    15841 tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg atgcgcagga
    15901 tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcgtacaccg
    15961 aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg caggtggatt
    16021 cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg cgcattctgc
    16081 aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg gacggaacgc
    16141 ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc actcatccgc
    16201 gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg ctgtatgtca
    16261 tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag ccgcgcatca
    16321 cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt gatttctgct
    16381 cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg caggaccgac
    16441 cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc
    16501 cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag gtgaactgga
    16561 ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg caggccattg
    16621 tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc cggggacagg
    16681 cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag accgtggact
    16741 tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa atctgcgatg
    16801 atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc cagacccgga
    16861 cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg ataagcctgg
    16921 ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg
    16981 tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg ctgaagctgc
    17041 cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac gacggcacgt
    17101 atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat aacggggcac
    17161 actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg gtgcagcatc
    17221 tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga tgggacacgc
    17281 cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg gacgacggca
    17341 gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc aggcagctgg
    17401 cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag cagggcgatc
    17461 cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt gaactgatac
    17521 cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg acggtgcagt
    17581 ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa accagcgccc
    17641 gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg ccgggccatg
    17701 attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt gtggaggccg
    17761 tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg ttgatcaata
    17821 aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt gcgccggacc
    17881 tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa accgtcaata
    17941 aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag gttgatacaa
    18001 ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac ggacgccttt
    18061 atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
    18121 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
    18181 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    18241 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    18301 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    18361 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    18421 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    18481 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    18541 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    18601 tcatgccgcc tgatagtgaa gaaaaacggt gcttaaattt ataaccgaac gaccctggat
    18661 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    18721 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    18781 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    18841 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    18901 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgcc
    18961 tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg cctcgacgaa
    19021 cgctcccggc agtgatgatc tgaacgggat taacgtgaaa taccgttatg agtttacgga
    19081 cacgctgggg ctggtgacgt cattcagcta tgccaacgct gaagatgagc aaaaaacgca
    19141 ttacagtgat acccgctggc atgaggattc cgtgcgtaac cgctggttca gcgtgatggc
    19201 ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gcgtggctta
    19261 cagccgtgtg tcgaccttct ccggggatta tctccgcgta actgacaaca aggggaaaac
    19321 gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc aacacgtctc tggcgtgggg
    19381 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gaccttgctt atgaaggttc
    19441 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggttata agttctgatt
    19501 agccaggtaa cacagtgtta tgacagcccg ctggttcagg cgggcttttt gtggggtgaa
    19561 tatggcagta aagatttcag gtgtactgaa agatggggcg ggtaaacctg tcgtaaattg
    19621 tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt gtggcacacg ttgttgccac
    19681 ttgcgtgacg gacaataacg gtgcttatgt gattgaggct gagccggggt attacgaggt
    19741 tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg gatattgatg tggcaccgac
    19801 tgatgcaccg gggacactga acgcgtttct gaatgcacca aaggatggtg atttacgtcc
    19861 ggaggtgatg aagcgctttg aggaaatggt ggcgcaggcg cagcagagtg ccggggctgc
    19921 agccggaaac gcacagcaga cggcgcagga tgtggcggca gccgcaacgg cccgtgatga
    19981 tgcacaacgt tttgcggaga aagcccgaca ggatgcaacc gtcacagctg aggacagaaa
    20041 ggccactgcg gaagatgtga caagcacagg agcaaatgca gccgcagccg gacagagcgc
    20101 acaggatgcc gcaggttatg cccgcgcagc agaacaggcc aaaaatgaca ttgatgctgc
    20161 gctgaccggc actctgaaaa tggctaacca tctgtc
//
LOCUS       DAJTNV010000076        20018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNV010000076 DAJTNV010000000
VERSION     DAJTNV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="OO199_002787"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="OO199_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5536763.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="OO199_002788"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="OO199_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5536764.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="OO199_002789"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="OO199_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5536765.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="OO199_002790"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="OO199_002790"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5536766.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="OO199_002791"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="OO199_002791"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5536767.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="OO199_002792"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="OO199_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="OO199_002793"
     CDS             complement(5911..6954)
                     /locus_tag="OO199_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5536768.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="OO199_002794"
     CDS             complement(7200..7652)
                     /locus_tag="OO199_002794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5536769.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="OO199_002795"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="OO199_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5536770.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="OO199_002796"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="OO199_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5536771.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="OO199_002797"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="OO199_002797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5536772.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="OO199_002798"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="OO199_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5536773.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="OO199_002799"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="OO199_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="OO199_002800"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="OO199_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5536774.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="OO199_002801"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="OO199_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13798..14538
                     /locus_tag="OO199_002802"
     CDS             13798..14538
                     /locus_tag="OO199_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5536775.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14509..15276)
                     /locus_tag="OO199_002803"
     CDS             complement(14509..15276)
                     /locus_tag="OO199_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5536776.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="OO199_002804"
     CDS             complement(15482..16060)
                     /gene="lpcA"
                     /locus_tag="OO199_002804"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5536777.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16300..18744
                     /gene="fadE"
                     /locus_tag="OO199_002805"
                     /pseudo
     CDS             16300..18744
                     /gene="fadE"
                     /locus_tag="OO199_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="OO199_002806"
     CDS             complement(18787..19233)
                     /gene="ivy"
                     /locus_tag="OO199_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5536778.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19414..>20018
                     /gene="yafV"
                     /locus_tag="OO199_002807"
     CDS             19414..>20018
                     /gene="yafV"
                     /locus_tag="OO199_002807"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5536779.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGC"
BASE COUNT         4888 a         5166 c         5120 g         4844 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
    13561 attaattgct tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac
    13621 atgcgctttt cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac
    13681 aggcttgcgt aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat
    13741 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
    13801 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
    13861 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
    13921 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg
    13981 ggcgagcaat atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg
    14041 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
    14101 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat
    14161 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc
    14221 ggctgctacg caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg
    14281 ctggtgtttg gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc
    14341 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc
    14401 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac
    14461 gtggtcagca agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg
    14521 ctccccgagg caaaataagt gccattcgcc tggcataatt ttttgccagg tttcattgcc
    14581 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
    14641 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
    14701 ccagtgtaaa ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt
    14761 gaaaacgccc ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc
    14821 catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc
    14881 tttttcgctg tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc
    14941 cgtcagttgt ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg
    15001 gtgagtattt tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga
    15061 acaggatttt atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg
    15121 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
    15181 atgtggcccg gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt
    15241 agggacgttg gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag
    15301 cgcatggacc ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgaa
    15361 ttattcggcc tacggttcgg gtacagattt gtaggcatga taagacgcgt cagcatcgca
    15421 tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc cttatccggc ctacgccaga
    15481 cttacttaac catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgaa
    15541 tctcctgaat gcggtcggca taaccaaagt gcggaacgcg aatttcgata tccgccgtgc
    15601 cagccatttt accgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg
    15661 ccgctgcgat cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta
    15721 catcgccttc gcgacctacc gcttcaacgt agcgggagaa aatatcattg aaaccgaaat
    15781 cattaccgac gcaggaaata tgactaacgt cagaaatagc aatcgccggg tagcccggac
    15841 ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg catagcgtcg caatgggaac
    15901 cgccgttgcc gcaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg
    15961 ccgcgcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg
    16021 ccgcttcgtt cagttcgtta cgaataagat cctggtacat gaggatatcc ttcagcataa
    16081 atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg
    16141 ctatgaccag gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt
    16201 tgtaattata ttgtaaacac attgctaaat gtttttacat ccactacaac catatcatca
    16261 caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc
    16321 gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt
    16381 ctgattttgc tcgcctagac agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta
    16441 ctggtgcctc tgaccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg
    16501 atttccgcgc cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag
    16561 aaagaagcga ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg
    16621 gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt
    16681 ctcgacggcc cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag
    16741 ctggcggatc tgccgccgga gttgtgggcg taccttaaag agcatcgctt cttcgcgatg
    16801 attatcaaaa aagagtacgg cggactggag ttctcagctt atgcccagtc tcgtgtgctg
    16861 caaaaactct ctggcgtgag cgggatcctg gcgattaccg tcggcgtgcc aaactcatta
    16921 ggcccgggcg aactgttgca acattacggc actgacgagc agaaagatca ctatctgccg
    16981 cgtctggcgc gtggtcagga gatcccctgc tttgcactga ccagcccgga agcgggttcc
    17041 gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag
    17101 gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc
    17161 gtgcttgggc tggcgtttaa actctccgac ccggaaaaat tactcggcgg tgcagaagat
    17221 ttaggcatta cctgtgcgct gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc
    17281 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgtg
    17341 ccgatcgatt acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg
    17401 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
    17461 tcggtagcgc tggcaacggg cgcgtattct cacattcgcc gtcagttcaa aatctctatc
    17521 ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg
    17581 gatgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg
    17641 tcggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg
    17701 gatattaccg gcggtaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac
    17761 cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg
    17821 atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
    17881 aagaacaatg acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt
    17941 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
    18001 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
    18061 ctgctttctg atgtctcgat ggcactgctg ggcggcagcc tgaaacgtcg tgagcgtatc
    18121 tcggcacgtc tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt
    18181 tatgacgacg aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg cgtacaggat
    18241 gcgctgtatc aggcagaaca ggcaatggac aatttactgc aaaacttccc gaaccgcgtg
    18301 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct
    18361 gacaagctgg atcataaagt ggcgaagatt ttacaagtgc cgaacgccac tcgttcacgt
    18421 attggtcgcg gtcagtacct gacgccaagc gagcataacc cggttggttt actggaagag
    18481 gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt
    18541 aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg
    18601 attgataaag atgaagccgc tattctggtg aaagcggaag aaagccgtct gcgcagtatt
    18661 aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa
    18721 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
    18781 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
    18841 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
    18901 ctcttgcgac gttttctcat caatagccga gaacagcccc gtcatctgat tagatttctc
    18961 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
    19021 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
    19081 cacccatgca ggtagcttat gcccttgcac catctgatta aacgcagctt tggtggtttc
    19141 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
    19201 cagtgcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
    19261 gttattaacc tcctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
    19321 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
    19381 tataaaaccc ttcgcataaa ggagttgtac accgtgccta gtttgaagat tacgcttttg
    19441 cagcaaccgt tggtgtgggt ggatggtcct gccaacctgc gccattttga tcgtcaactg
    19501 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
    19561 atggaagcgg cagcttcgtc gcttgcacaa gatgacgtag tgaactggat gacagctaag
    19621 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg
    19681 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
    19741 ctgttccgca tggcagatga gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg
    19801 gaatggcgcg gctggcgtat tttgccgctg gtgtgctacg acttacgttt tccagtgtgg
    19861 tcgcgcaatc tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc agctccgcgc
    19921 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
    19981 ggatgcaatc gcgtcggcag cgatggcaac ggctgcca
//
LOCUS       DAJTNV010000077        19414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNV010000077 DAJTNV010000000
VERSION     DAJTNV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="OO199_002808"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="OO199_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="OO199_002809"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="OO199_002809"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5536780.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO199_002810"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OO199_002810"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5536781.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="OO199_002811"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="OO199_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5536782.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="OO199_002812"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="OO199_002812"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5536783.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="OO199_002813"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="OO199_002813"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5536784.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="OO199_002814"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="OO199_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5536785.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="OO199_002815"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="OO199_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5536786.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="OO199_002816"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="OO199_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5536787.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="OO199_002817"
     CDS             11402..11617
                     /locus_tag="OO199_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5536788.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO199_002818"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OO199_002818"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5536789.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="OO199_002819"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="OO199_002819"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5536790.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="OO199_002820"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="OO199_002820"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5536791.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="OO199_002821"
     CDS             complement(16353..16892)
                     /locus_tag="OO199_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5536792.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="OO199_002822"
     CDS             complement(16908..17426)
                     /locus_tag="OO199_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5536793.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="OO199_002823"
     CDS             complement(17737..17928)
                     /locus_tag="OO199_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5536794.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="OO199_002824"
     CDS             17946..18098
                     /locus_tag="OO199_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536795.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO199_002825"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="OO199_002825"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5536796.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5278 g         4839 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAJTNV010000078        19375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNV010000078 DAJTNV010000000
VERSION     DAJTNV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO199_002826"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO199_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5536797.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO199_002827"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO199_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5536798.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO199_002828"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO199_002828"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5536799.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO199_002829"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO199_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5536800.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO199_002830"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO199_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5536801.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO199_002831"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO199_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5536802.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO199_002832"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO199_002832"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5536803.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO199_002833"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO199_002833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5536804.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO199_002834"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO199_002834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5536805.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO199_002835"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO199_002835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5536806.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO199_002836"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO199_002836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5536807.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO199_002837"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO199_002837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5536808.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO199_002838"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO199_002838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5536809.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO199_002839"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO199_002839"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5536810.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO199_002840"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO199_002840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5536811.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO199_002841"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO199_002841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5536812.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO199_002842"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO199_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5536813.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO199_002843"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO199_002843"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5536814.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAJTNV010000079        18904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNV010000079 DAJTNV010000000
VERSION     DAJTNV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            21..320
                     /locus_tag="OO199_002844"
     CDS             21..320
                     /locus_tag="OO199_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5536815.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            317..2065
                     /locus_tag="OO199_002845"
     CDS             317..2065
                     /locus_tag="OO199_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5536816.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2354..2632
                     /locus_tag="OO199_002846"
     CDS             2354..2632
                     /locus_tag="OO199_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536817.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2629..2934
                     /locus_tag="OO199_002847"
     CDS             2629..2934
                     /locus_tag="OO199_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536818.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2944..3105
                     /locus_tag="OO199_002848"
     CDS             2944..3105
                     /locus_tag="OO199_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5536819.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3247..3471
                     /locus_tag="OO199_002849"
     CDS             3247..3471
                     /locus_tag="OO199_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536820.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3772..4929
                     /locus_tag="OO199_002850"
                     /pseudo
     CDS             3772..4929
                     /locus_tag="OO199_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4969..5541
                     /locus_tag="OO199_002851"
     CDS             4969..5541
                     /locus_tag="OO199_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5536821.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5543..6754
                     /locus_tag="OO199_002852"
     CDS             5543..6754
                     /locus_tag="OO199_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5536822.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6751..7089
                     /locus_tag="OO199_002853"
     CDS             6751..7089
                     /locus_tag="OO199_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5536823.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7086..7382
                     /locus_tag="OO199_002854"
     CDS             7086..7382
                     /locus_tag="OO199_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5536824.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7382..7822
                     /locus_tag="OO199_002855"
     CDS             7382..7822
                     /locus_tag="OO199_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5536825.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7806..7988
                     /locus_tag="OO199_002856"
     CDS             7806..7988
                     /locus_tag="OO199_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536826.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8044..8334
                     /locus_tag="OO199_002857"
     CDS             8044..8334
                     /locus_tag="OO199_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5536827.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8354..10015
                     /locus_tag="OO199_002858"
     CDS             8354..10015
                     /locus_tag="OO199_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5536828.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10029..10310
                     /locus_tag="OO199_002859"
     CDS             10029..10310
                     /locus_tag="OO199_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536829.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11107..12567)
                     /gene="phoQ"
                     /locus_tag="OO199_002860"
     CDS             complement(11107..12567)
                     /gene="phoQ"
                     /locus_tag="OO199_002860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5536830.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12567..13238)
                     /gene="phoP"
                     /locus_tag="OO199_002861"
     CDS             complement(12567..13238)
                     /gene="phoP"
                     /locus_tag="OO199_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5536831.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13407..14777)
                     /gene="purB"
                     /locus_tag="OO199_002862"
     CDS             complement(13407..14777)
                     /gene="purB"
                     /locus_tag="OO199_002862"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5536832.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14781..15422)
                     /gene="hflD"
                     /locus_tag="OO199_002863"
     CDS             complement(14781..15422)
                     /gene="hflD"
                     /locus_tag="OO199_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5536833.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15458..16564)
                     /gene="mnmA"
                     /locus_tag="OO199_002864"
     CDS             complement(15458..16564)
                     /gene="mnmA"
                     /locus_tag="OO199_002864"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5536834.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16618..17079)
                     /gene="nudJ"
                     /locus_tag="OO199_002865"
     CDS             complement(16618..17079)
                     /gene="nudJ"
                     /locus_tag="OO199_002865"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5536835.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17089..17742)
                     /gene="rluE"
                     /locus_tag="OO199_002866"
     CDS             complement(17089..17742)
                     /gene="rluE"
                     /locus_tag="OO199_002866"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5536836.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17914..>18904
                     /gene="icd"
                     /locus_tag="OO199_002867"
     CDS             17914..>18904
                     /gene="icd"
                     /locus_tag="OO199_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5536837.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGD"
BASE COUNT         4910 a         4653 c         5075 g         4266 t
ORIGIN      
        1 cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca
       61 ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc atggtaacga
      121 ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa tatgattgcg
      181 tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg ccccgcagta
      241 aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag atgcttaacg
      301 tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac gcggaataaa
      361 acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc acgcgaaaca
      421 atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt ggcttgggcc
      481 aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa aacgcgtaag
      541 gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc agaagctggc
      601 ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc agaagtgtga
      661 aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc ttaccatgcc
      721 ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc agctgcttgc
      781 cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg tatggcgcgg
      841 cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg cgatctattc
      901 agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag cgttaaaagt
      961 tattgccgaa cccatgggcg agccgtccgg cgatttgctg ccgttcgcca atggtgcgct
     1021 tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca ccacgcacaa
     1081 cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg cgctaaactt
     1141 tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc gtatatgtgc
     1201 cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg aggccaccgg
     1261 agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg cagggaaaca
     1321 gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg cgcaggttgt
     1381 cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag gaacgggcat
     1441 caagaaaatc acgggcggtg accccgcgga aattaacccg aaatacgaaa agcgtttcac
     1501 ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat tcaccgaacg
     1561 ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg ttagtgaggc
     1621 cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca ttatccgccg
     1681 attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg aacagcgtga
     1741 cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt tttgccagtt
     1801 cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg attcagtgaa
     1861 gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt acgcaggcag
     1921 gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg cgaaagttta
     1981 cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg caataacaac
     2041 agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctaccctg tctacctgag
     2101 taaagaaaaa tacatttaat tcagtgcatt aagttaggta gatagccttt ttttactgtc
     2161 tacccactat ctaccctctc tacctgattt tacctgaatc agacagggag gtagacacgg
     2221 ggtagatagt ggataaaagc actctacccc gctgaaagca gcgccattac tggcatgagg
     2281 gccactaagg tagataaggt agacaagggg aggcacaact caaaactttt taaacgaggg
     2341 ggtaaaaata aatatgcaca cttcagggaa atttaataaa tcattcaaaa aacgcagaga
     2401 cagaacagaa ccgaaatatc gcgcgttaga catgacagag cacgctttaa aggtggcaat
     2461 cagaacgata gaccgccacg cgggggaagg atacgcgaaa gcacatcccg aactgataag
     2521 cgcattcatg accacgacgg cggcaaattt tgccacgctg acagagcggg agattgccga
     2581 agcggaacag gtaacaacaa tcaacgttaa aaccggagag gtggaatcat gagcgaacca
     2641 aaatttggtg aaaaatttta caagcacaac ggacggatca cgattctgca aataagcgca
     2701 gctacaccag gctggtgggt tgaaacagac gaaggatcct cacccgtggc atcatgggcg
     2761 ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg atattctgcc tgtgatatcc
     2821 actgacaaag gcatgaaacc cgtagacatc aaaaacatgg ggtttcagtg cgtaatgctc
     2881 actgaaaaaa tgatggaaga gatgaaaaag aacaatgccg gatcgcttca ctgaggtggc
     2941 aatatgacag cacaaattgc agcttacggg cggctggtgg gcgacccgct ggtaaaacag
     3001 agcagcaagg gcacaccgga gccgccaacg gagaactacg cagacctgag aaatgcagta
     3061 aatcgcactt acgcagacat ggggctgagc aaattagcag agtaggaata tcagaaccac
     3121 accacgcagc cggagcaatc cggctttttt tgcgtcattt gaaacggtat ggagcactac
     3181 tggcttttcg tcacggtccg gcatagttac tatctgaaac aaacagacac aacagaggaa
     3241 aacgcaatgc cgatgaaatt tgatgagata cttaaacagc gtgataaata ccatgctgac
     3301 aacatggaga cgatgaatat cactgattac cgcgcatttc tggaaacggg ggcgctgatt
     3361 gaaaaggatc atcatggttt tgttcgttgc gcgctatccg gtgaaatgct ggcagtaaat
     3421 cctgaacaga cagatgcatt gatagaattt ctgaaaggga tcagagactg agcacccata
     3481 cagcacatac agccggagca atccggcttt ttgttatttt ttgtaaatta tttgttcgtg
     3541 gttgttccac gttgttcact gagcggatcg gcatatttta cccgaactga atcatgatta
     3601 ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg tgctcgccaa
     3661 tattcattac caggcgggaa aacgatcggt gcgattactg atttccttat gaaaaacggt
     3721 tgagtgtttg ccgcgtcctg gagttcctta cttaacccca ggactttttt tatgcagaga
     3781 ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga catgctggag
     3841 aacgctgaaa aagaaaaccg cagtcttaac gatgctgagg gcacaaaatt tgacgaatta
     3901 cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat tgcagacgaa
     3961 gagcgcagca agccaggtaa aagcagccag accactgacc ccgcagaact acgcaactac
     4021 attctgaccg gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg tggttatacc
     4081 gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc caccatgcgt
     4141 cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt ttccgctggc
     4201 ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag caccccgcag
     4261 attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac cacacaggaa
     4321 atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat tggcgacacc
     4381 ttcacggaaa ccgaagaaag cgatctggtt gtgggcgacg gtgacaaaaa agcaaaaggt
     4441 tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg tacgttgcag
     4501 gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat cgacctgaaa
     4561 tttgcattac gtaaaaaata ccgcaaaaac gcggtctgag tggttaactc cacgacggcg
     4621 gcaaaacttc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga ccgtttacag
     4681 acgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt tatgcctgat
     4741 aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca cgaaacaggt
     4801 gttcgcacca gaccggacaa cctgacagaa ccaggattca tcaaaatttt cacgcaaaaa
     4861 tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggcact gccggaagac
     4921 gacgattaac agcatacaga aggggctaaa aagccccttt agtgttttat gggtgaaaaa
     4981 attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggagc cggtacgctg
     5041 acgggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga gttttacgaa
     5101 aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt tcgcggcctg
     5161 tatgagcatg accacagcat gttactgggg cgcacccgtt ccggcacgct gaaactggaa
     5221 gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagtac agggcgcgac
     5281 gttatcgaac tggttaagcg cggggacatc tcggggatga gctttggctt tcgctcccgt
     5341 aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt ggcggaactg
     5401 tacgaaatta ccgttacatc ggtaccggct taccccgatt ccggcgtgga gctggcccgc
     5461 cgttccctgt atgagcagca ccccgaaaaa atgccgcgtg cggataatcg ccgctggtgg
     5521 gcggatttag cgggggtgtg atatgtggcc tttcagacga aaaaaagagc agcgcagcat
     5581 gacgcttgat gaatttatgg cgctggccgg cacatcgaac acgggggcgg gtgagtacgt
     5641 atcatctggg acagcggaat cactccccgc cgtcatgaac gccgtcacgg tcatctctga
     5701 cgcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg ggaaggaggc
     5761 ccgcgaatgg cttgattctc atccggtcga tcacatcctc aacgagcgcc cgaacgcgtg
     5821 gcagacaccc taccagttta agcgaatgat gatccgctac tgcctgttaa gtggtaatgc
     5881 ttatgcggtt attcagtggg ggcgtgatgg ttttccggcg gctttacatc cttacccgcc
     5941 gcagtcggtg aacgtggagc agacaggtga tcataactgg cgctactgca tcactgacgc
     6001 ctacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc gttactccac
     6061 ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc tggggcttgg
     6121 gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg cggcaggggt
     6181 tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag ccgcactgga
     6241 acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg ggatgagcta
     6301 tcagcagctg ggcatgagta atcaggatgc cgaatggctg gcctcccgtc gcttcaccat
     6361 tgaagacatc gcccgaatgt tcaacgtctc gccgattttt ttgcaggaat acagcaacag
     6421 cacctacagc aacttcagcg aggcaagccg cgcatttctc accatgacga tgcgcccgtg
     6481 gctggcgaac tttgagcagc agataaaaaa cgccttgctg gtggcctcgc ctgtacctgg
     6541 tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac ctggcgaacg
     6601 ctttgccacc tatgagcgcg gcataaaatc cgacgttatg tgtccgaacg aagcccgcga
     6661 acgtgaaggg ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat ggaaacagga
     6721 agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg tgacaggata
     6781 acgattcaga ccctgaaaca gaccagggat ataaccggcg aaatactcga aacgtgggag
     6841 gacggtcaca cactctgggc aagcgtgaac atggtcagca gcaaggaggc catttcatcg
     6901 ggcgcagagc tggcgattgg taccgtaagg atctggatac ggtaccggaa ggacatcaac
     6961 gccaccagcc ggataaaggt cagtacgggg ccgctggcgg ggcgtgtact gaatatcatc
     7021 gggcagccgc tgccggatgc cgccaggaca cgccttgaaa ttctttgtcg tgagggcgcg
     7081 gaaaaatgac agaagaactt atcaccctgg aagaagtgaa actccattgc cgcatcgatg
     7141 gcgacgatga agaccagtta atcagcggat acattgccgc atcgcttgag gcgtgccaga
     7201 tacacatagg caggcgcttt gatgacgggc tggcgttcac gccagccata aagattggct
     7261 gcatgatgtt tatcgctcac ctgtatgaaa atcgccagat tgtcgcggat aacgcaaaaa
     7321 cacgcgtacc catgacgata ggcgcactct ggacggctta ccgtgatgtg ggggggtact
     7381 gatgccgtgg caaccattaa gacgatgcac agaaccaggc tgtaacaggc gcgttaaatc
     7441 cggcaagtgt gaggagcaca ggcgggcggc atggcgtgca gaggatgcca gacgaggaca
     7501 ccgccgcgcg cgtggatatt ccagacagtg ggacaaatac cgcgccatgt atctgagtaa
     7561 aaacccgtta tgcgtgcgat gccttgagaa ggggatatat acgcccgccg tggtggtgga
     7621 tcacattatc ccgattaatg gcggtgatga tgttctcttc tggcccgaat ggaaccatca
     7681 accattgtgc caggcgtgtc ataaccagaa aacgaaatgg cttgatccgg caacaaaaag
     7741 caagcgtgcc gcaggtggat ttcgtgaaga ggaagaacgg gctgctaacc gcaataactg
     7801 gatgtatgac gctgatgaat gagcgggaac aaaaccgcct tatccgtgga cttataaggc
     7861 agcgtgacgc atggaagaca caggaaacag ggcataaaga taaagcgtca ggacgcgcag
     7921 aacgcatcac agcgaagcga ttaaccgacc gtgaccgcga ggttatggaa tgtttccgca
     7981 atcgctgatg aggctgtatg acggggtggg gggcgttttc aggacaaacc cgaccccgcc
     8041 tacctggatg atatcgccgt gaagcagtgg cgggaaaaat cgcggcagct tgcggaacag
     8101 ggagacctga cccccgccga ctggagcaat ctggaactgt attgcgtcaa ctactccatt
     8161 taccggaaag ccgttgcaga ccttgcggcg cgcgggttca gcattgttaa cagtcagggc
     8221 ggcgagagca gaaaccccgc attaagcgca aaatccgacg ctgaaagagt gatgataaaa
     8281 atggcctcct tgctcggttt tgacccgata agccgccgta aaaatccacc gtaaacagaa
     8341 gaagaggacg agcttgaccg cctggaataa gtacgcagaa gacgtaaaaa cgggcaaaat
     8401 tccggcctgt aaacggttaa aacaggctgt taaacggtac ttttcggacc ttaaaagccc
     8461 cctttacacg ttcgatcgtg aggttgtgga gcggtttatt gccttttcca gggtgtgccc
     8521 gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc agcagttcgc
     8581 ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat acacgagcgc
     8641 ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc tggctaactg
     8701 gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga gccgtgacca
     8761 ggcgcggatc gtgtttgata atgcgcgtca gatgtgcctt ttatcccgac cgttacgcag
     8821 gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc tgttaaagcc
     8881 gctggcagca aaagcggcaa ccatcgaggg gacaaacccc agtctttcca tcgtggacga
     8941 ataccaccta caccccgata acggcgttta ttccgcgctt gagctgggga tgggcgcacg
     9001 tcccgaaggg ctgttatttg ccattaccac atcgggcagt aacgtcgttt cagcctgtaa
     9061 acagcactat gattattgct gccagatcct ggacggcgaa gaggtcaacg attcaatttt
     9121 tgtactgatt tacgaactgg acgacgaaaa cgaggttgat gatccggcga tgtggataaa
     9181 ggcgaaccct aatattgatg tttccgtgga tcgtgaaaaa ctggcctcaa ccatccagaa
     9241 agcgcggggt attccgtcgc agtgggtgga gatgatgaca aagcgattta atatctggtg
     9301 tcaggggact accccgtgga tgggtaatgg tgcatgggct gagtgtgccg gaacgttcac
     9361 ggaggaagat ttacacggtc aggagtgtta cgcggggctg gacttatcat caaccagcga
     9421 tatttccagc gtatgctatg cctttccggt cggtaaaacc attatgctga tttcccgtca
     9481 ttatctgccg gagttccagc tacagaaccc cgccaataaa aaccgcgctg tctatcgcca
     9541 gtgggctaaa gcgggctgga tacgcacaac gccaggtgac tgcattgatt acgaccggat
     9601 cagagatgac atcatgcagg atgcggagaa atttaatatc aggctggtgg gctttgatac
     9661 gtggaacgcc acgcatctca ggacgcaatt acagggggct ggttttgagg tggagccgtt
     9721 cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg ttcgaagttt ttgttaaccg
     9781 cagggtgatt gtgcatcgtg gcgatccggt gttgtcctgg tcgatgagta acgtcgtgat
     9841 gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcaccga acaagataga
     9901 cccgagcgta gcggcgctga tggcgtttgg tacattccag gcagagcatg aggattttgc
     9961 ttttgatata agcgacagcc accgccagaa actggaagaa tttagcgggg tgtaatgagg
    10021 tcagcagcat gacagaagcc gaaatactgc gattaatccg ccgcgtctct ggaatcagcc
    10081 agcaggctga cgaacagacc acgcagccgg acagcgtgac agccgaaaat tatgcgcgtg
    10141 ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa tgatgcggat atgcgcgaca
    10201 tacggatccg cgttctggaa atgctggcct acaatcgccg cgttgaactg tatcgggaga
    10261 aggagaaaat cacgtaccac tggaagaagc cggagcggtt acggcggtaa ctcactgaga
    10321 ttaaagcaaa gcgcaaaatt gcgctggcta catccctcac atctgaggac tgttaaccag
    10381 caggccggaa tatattcaga aattacgaaa ttttcgcagt ttaattactc gtcagcttaa
    10441 tcgctaaccc gctctgatat tttcggaaac ctcaatttga ggaagtcggc gaggtaactg
    10501 tttgatattc ccgatagcgc aaaaatgcgc ggtcgggcag aatgaccacc acctcaatcg
    10561 tgacggagag tgacaaccag tgacacaccg tgacaaaaag acggtttaac cgttcattaa
    10621 aaatcgacgt gattttgtgc tttccggatg acgtggccaa catcattttt tagcgaaaaa
    10681 ttctgattaa ggtcactggc tgaacggtca taatgaccac gcagaccatc gaggcaacca
    10741 tagcgatcat cgttacggtt ggagcttttc cgccattggt ggaaaaggtg cacatctgga
    10801 tcatcattac ggtgatcaag ggtatgtaaa acccaccagc ctgattaaag gttaaccgga
    10861 aaaaaaatcc ggtagccccg ttatggggat ccccatatcg acattaacgc ccctcatgaa
    10921 ttggtgcgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc tatttgttcc
    10981 acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagtcaat ttgcgatttt
    11041 tggcactttt tcaatgaaaa ttgtttgatc gaatcaatga cttgatgtag tggtaaaagg
    11101 acatatttat tcatctttcg gcgcagaatg ctggcgacca aaaatcacct ccatccgcgc
    11161 accgcccagc atgctctctc cggcgacgat tttaccctca tattgctcgg tgatttcgcg
    11221 ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt gaccacggtc
    11281 gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga ccacaatata
    11341 gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat atttacaggc
    11401 attatccagc acgttgccca tcacctcgac aaaatcgttc tgctcaccga caaagctgat
    11461 ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt tgttcagcgc
    11521 tgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga gcaatgtccc
    11581 gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc ggctgatttg
    11641 ctccagcatt accggctcag catcactgac gctcatcttt tcactacgca gagaacgcag
    11701 cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg tgagcgtcgt
    11761 acggtatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt ttcgtaccag
    11821 actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt cttccagttc
    11881 gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg cgacccacag
    11941 cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc tccagaccat
    12001 ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta attttggcat
    12061 ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg cgtcgtcatc
    12121 atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca gcaagaggct
    12181 ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc agtcaggctg
    12241 gatcattttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc cgttctcatc
    12301 ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact cgacatgcaa
    12361 cttattgttt tcccactgcg caagggtata gaacagattg ctctcgccac gtaacagccg
    12421 aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc cgtaggcaag
    12481 cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca gcgagagcgg
    12541 gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaggtagcc ctggccgcga
    12601 acggtggtaa tgacttcttg cggatattgt gcctgaattt ttttgcgcag acgtcccatc
    12661 agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg tagcattaac
    12721 gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat ggtgtattcg
    12781 aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga gagatcaacc
    12841 tgaaacgggg ggagcgaaat gacctgtgaa gccagaccgc tattacgccg cattaatgcc
    12901 tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc atcagcaccg
    12961 gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac cagaatcggc
    13021 agtgaaacat cgttgctacg ccagcggcga atcagtgaca gaccgtcctc gtcaggcaat
    13081 ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc ggcttctttg
    13141 gcatcttctg cgtcatcgac ctgatgacca gcatcctgaa tctgaacttt aaggtggtga
    13201 cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc ctgtcttaat
    13261 tattaaaata gtgtaacgcg attatgtgtt tatgggggta aacattaaat aaaccagcgg
    13321 ggaggggagg taaagtgaaa aaagaaaaag tggataatct taataagcag gccggacagc
    13381 atcgccatcc ggcactgatt tgaggcttat ttcagctcat caaccatcgt gatagctcga
    13441 ccaatatagt tagccggcgt catcgctttc aggcgggctt tctcttcttc tggcaacgcc
    13501 agaccatcga taaactgctt catgccttcg gcgtcaacgc gcttaccgcg agtaagctct
    13561 ttcagcttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg gattggttct
    13621 gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt cacttccagt
    13681 ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat acccacgccg
    13741 aggttacgca gcacggtgga gtcggtcagg tcacgctgcc agcgggaaac cggcagtttg
    13801 cttgccagat gctgcaatac cgcgttggaa aggcccagat tcccttcgga gttttcgaag
    13861 tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccagc aatggttttc
    13921 tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc gatcagaatg
    13981 gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg ttcaatctgg
    14041 gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct gaactgatgc
    14101 cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc gccgttgatt
    14161 ttgccgagga tctccacctg gttaagctgg cggtgctggc gctccatacg gtaggcgacg
    14221 tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt acgagacagc
    14281 agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaatcag ttgacgccag
    14341 tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga gaggttattg
    14401 atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag ttccgggatc
    14461 tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt agtggtacgc
    14521 tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat tgcatcaagg
    14581 taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg cgcggccagt
    14641 ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata ttcgctgaaa
    14701 atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga aacggcggtc
    14761 agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat ttgttttgcc
    14821 tgagtggtca ggcgattacg agaaaacatc agttgtagac gtccgccgcc gacctggtgc
    14881 cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc ctgcacttgt
    14941 gggctttgca gtacagcagg ggaaccggtg acctgaatgc gcgggccaag cgggctgatc
    15001 acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa atcgaagtgt
    15061 tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc gcctttcgct
    15121 gaggagagtt tgcgctcaag caccatcaag ctgagtgtgt agcgggttaa ttcggcgttt
    15181 aagccctggc aactgctggc attgagcacg ccgagcaggg tttccagccc gacgcgcagg
    15241 ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc aataatactg
    15301 ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag ttgttgcacc
    15361 aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta attctttgcc
    15421 acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc ggcagacgct
    15481 gctcaataat accgccaccg aggcacactt caccgttata gaagacggca gactggcccg
    15541 gcgtcacggc ggcaaccggc tcatcgaaaa tcactttaat gcgatcgtcg tccagcgcct
    15601 tgacggtgca cggaatgtcg gtctggcgat agcgggtttt taccgtgcaa cgcatagtgc
    15661 cggtgaatgg ttcgcgatcg acccagtgca gctgctgggc aatcaacccg acagacatca
    15721 gccgcgggtg ttcatggccc tgagcgacaa ccagaatgtt gttttcgacg tctttgtcca
    15781 ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga cctttacgct
    15841 gaccgagagt gtgatacatc agcccctggt gctcaccaat ttcatcgcca tcgacggtaa
    15901 tgattttgcc cggttgcgcc gggagataac ggcccaggaa ctcgcggaat ttacgttcgc
    15961 caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca agatcttcag
    16021 caatcttacg cacctgcggt ttttccagtt cgcctaccgg gaacaggctt tgcgcaatct
    16081 gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc agaccacgca
    16141 gcaggcggct cttgccatcg acatcggccc gacgtacgta atgaccggta gcgatataat
    16201 cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc tccttgttgc
    16261 acagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac agttcgaaga
    16321 cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg agcttgtcgc
    16381 agaccgcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca ccgtcgtctt
    16441 cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc aacagccagg
    16501 cagaaacgga ggaatcgaca ccgccggaca tgccgacgat tacttttttt gcggtttcag
    16561 acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg cagcctttta
    16621 gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg gataacgttg
    16681 cccgctttga taacaacgaa tactttccgc caccagcggc gaacgaagat ttgacgcctg
    16741 taaaatttct tcggcgctga cccaacggca gcagtcgata tcgctgtcat gaggctgggt
    16801 cgggcatatt tgctcaagct caatggcaaa gaggaaacgc aaaaacggcg ttttatctgg
    16861 cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc cggtttcttc
    16921 ccacagttca cgggcggcgg cttccactaa ggtttcatcg gcttccagat gcccggcagg
    16981 ttggttccat aacgctttac cattaatcgt ctcttcaacg actaaaaatt tgccttctgc
    17041 gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt aatctgtcac
    17101 ttctcgccat tcgccattgg caagattatc caaagagtaa tctcccatcg catagcgaat
    17161 cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc gattacgtcc
    17221 ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac gaattggtgg
    17281 attccgcggc cataaccacg cgggttcgtc aaccagctcc gcgcctgcgg gcagggtagg
    17341 gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg tgggaatacc
    17401 ttccacctgc acataataga tttttccggt gcgtttaccc ggctgggtta aacgcgcctg
    17461 caacgcgccg ttattggtca gcaccagcaa cccttcgcta tcgcggtcaa ggcgacctgc
    17521 tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg tttcatcggt
    17581 gaactgcgga agaacatcgt agggtttatt gaagaggatc acacgcgtgg gctggttttc
    17641 atgtttacgc ctggtagaac gttgtgagct gaatcgctta acctggtgat ttctaaaaga
    17701 agttttttgc atggtatttt cagagattat gaattgccgc attatagcct aataacgcgc
    17761 atctttcatg acggcaaaca atagggtagt attgacaagt caattacaaa tcattaacaa
    17821 aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg tggtggcaga
    17881 cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt tgttccggca
    17941 caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa tccgattatc
    18001 ccttacattg aaggtgatgg aatcggtgta gatgtaaccc cagccatgct gaaagtggtc
    18061 gacgctgcag tcgagaaagc ctataaaggc gagcgtaaaa tctcctggat ggaaatttac
    18121 accggtgaaa aatccacaca ggtttatggt caggatgtct ggctgcctgc tgaaaccctt
    18181 gatctgattc gtgaatatcg cgttgccatt aaaggcccgc tgaccactcc ggttggtggc
    18241 ggtattcgtt ctctcaacgt tgccctgcgt caggaactgg atctctacat ctgcctgcgt
    18301 ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact gaccgatatg
    18361 gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa agctgactct
    18421 gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa gaaaattcgc
    18481 ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac caaacgtctg
    18541 gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac cctggtgcac
    18601 aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta ccagctggcg
    18661 cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt taaaaacccg
    18721 aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct gcaacagatc
    18781 ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg tgactacatt
    18841 tctgacgctc tggcagcgca ggttggcggt atcggtatcg cccctggagc aaacatcggt
    18901 gacg
//
LOCUS       DAJTNV010000080        17173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNV010000080 DAJTNV010000000
VERSION     DAJTNV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..185
                     /locus_tag="OO199_002868"
     CDS             <1..185
                     /locus_tag="OO199_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5536838.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OO199_002869"
     CDS             complement(210..401)
                     /locus_tag="OO199_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5536839.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OO199_002870"
     CDS             complement(506..742)
                     /locus_tag="OO199_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5536840.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO199_002871"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO199_002871"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5536841.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO199_002872"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO199_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO199_002873"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO199_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5536842.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OO199_002874"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OO199_002874"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5536843.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OO199_002875"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OO199_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5536844.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OO199_002876"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OO199_002876"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5536845.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OO199_002877"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OO199_002877"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5536846.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OO199_002878"
     CDS             complement(10860..12233)
                     /locus_tag="OO199_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5536847.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OO199_002879"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OO199_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5536848.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OO199_002880"
     CDS             complement(13344..13583)
                     /locus_tag="OO199_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536849.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OO199_002881"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OO199_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5536850.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OO199_002882"
     CDS             13960..14247
                     /locus_tag="OO199_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5536851.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="OO199_002883"
     CDS             14826..14969
                     /locus_tag="OO199_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5536852.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="OO199_002884"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="OO199_002884"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5536853.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="OO199_002885"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="OO199_002885"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5536854.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="OO199_002886"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="OO199_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5536855.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4533 a         4154 c         4336 g         4150 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatgag
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggtctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAJTNV010000081        17030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNV010000081 DAJTNV010000000
VERSION     DAJTNV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(52..306)
                     /locus_tag="OO199_002887"
     CDS             complement(52..306)
                     /locus_tag="OO199_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5536856.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            616..2388
                     /gene="aspS"
                     /locus_tag="OO199_002888"
     CDS             616..2388
                     /gene="aspS"
                     /locus_tag="OO199_002888"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5536857.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2506..2958
                     /gene="nudB"
                     /locus_tag="OO199_002889"
     CDS             2506..2958
                     /gene="nudB"
                     /locus_tag="OO199_002889"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5536858.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2987..3727
                     /locus_tag="OO199_002890"
     CDS             2987..3727
                     /locus_tag="OO199_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5536859.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3762..4283
                     /gene="ruvC"
                     /locus_tag="OO199_002891"
     CDS             3762..4283
                     /gene="ruvC"
                     /locus_tag="OO199_002891"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5536860.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4285..4887)
                     /locus_tag="OO199_002892"
     CDS             complement(4285..4887)
                     /locus_tag="OO199_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5536861.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4956..5021
                     /gene="yobI"
                     /locus_tag="OO199_002893"
     CDS             4956..5021
                     /gene="yobI"
                     /locus_tag="OO199_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5536862.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5160..5771
                     /gene="ruvA"
                     /locus_tag="OO199_002894"
     CDS             5160..5771
                     /gene="ruvA"
                     /locus_tag="OO199_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5536863.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5780..6790
                     /gene="ruvB"
                     /locus_tag="OO199_002895"
     CDS             5780..6790
                     /gene="ruvB"
                     /locus_tag="OO199_002895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5536864.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="OO199_002896"
     CDS             complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="OO199_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5536865.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="OO199_002897"
     CDS             complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="OO199_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5536866.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8553..9485
                     /gene="znuA"
                     /locus_tag="OO199_002898"
     CDS             8553..9485
                     /gene="znuA"
                     /locus_tag="OO199_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5536867.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9501..10823
                     /gene="mepM"
                     /locus_tag="OO199_002899"
     CDS             9501..10823
                     /gene="mepM"
                     /locus_tag="OO199_002899"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5536868.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10943..11914
                     /gene="lpxM"
                     /locus_tag="OO199_002900"
                     /gene_synonym="msbB"
     CDS             10943..11914
                     /gene="lpxM"
                     /locus_tag="OO199_002900"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5536869.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="OO199_002901"
     CDS             complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="OO199_002901"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5536870.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="OO199_002902"
                     /gene_synonym="yebK"
     CDS             complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="OO199_002902"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5536871.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14821..16296
                     /gene="zwf"
                     /locus_tag="OO199_002903"
     CDS             14821..16296
                     /gene="zwf"
                     /locus_tag="OO199_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5536872.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16531..>17030
                     /locus_tag="OO199_002904"
     CDS             16531..>17030
                     /locus_tag="OO199_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5536873.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALS"
BASE COUNT         4349 a         4206 c         4429 g         4046 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg ggcatcaacc ggctgtttta atgcttcggc
      121 gtaatcggca gaccagccaa ctcgcaccag aaacacgggc tgaccgctgg cgcgaaattt
      181 cgccgccagc ttcccggcgc gattaactac ctcatcggca gtatgtggac caccggcaaa
      241 aggtaagatg ccttcttgta aatcgatcac caccaacgca gtggttttag cattaagttc
      301 aagcatgctg actcctgaaa aaacaaaatc gtgcctcaca ccttaatgcg attagccgat
      361 ggcggaacga caaattttgt taatttttat gagttcgggc aacaccttta tagcaaactg
      421 gcgtagagtc tgatttcagg cccgatataa agtggtaacg aggcgttcca gcgcgggcca
      481 ggctcttacg gtgcggcaga atttccagta taatagccgc cttttttcat tcagttgtga
      541 catacagcta acgctgcgac ttggtcacct gcgcagcagg gggcatccgc cggcagctaa
      601 gttaagggat atctcatgcg tacagaatat tgtggacagc tccgtttgtc ccacgtgggg
      661 cagcaggtga ctctgtgtgg ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc
      721 atcgatatgc gcgaccgcga aggtatcgtg caggtatttt tcgatccgga tcgtgcggac
      781 gcgttaaagc tggcctctga actgcgtaat gagttctgca ttcaggttac gggcaccgta
      841 cgtgcgcgtg acgaaaaaaa tattaaccgc gatatggcga ccggcgaaat tgaagtgctg
      901 gcgtcctcgc tgactatcat caaccgcgca gatgttctgc cgcttgactc taaccacgtc
      961 aacaccgaag aagcgcgtct gaaataccgc tacctcgacc tgcgtcgccc ggaaatggcg
     1021 cagcgcctga aaacccgcgc taaaatcacc agcctggtgc gccgttttat ggatgaccac
     1081 ggcttcctcg acatcgaaac tccgatgctg accaaagcca cgccggaagg cgcgcgtgac
     1141 tacctggtgc cttctcgtgt gcacaaaggt aaattctacg cactgccgca atccccacag
     1201 ttgttcaaac agctgctgat gatgtccggt tttgaccgtt actatcagat cgttaaatgc
     1261 ttccgtgacg aagacctgcg cgctgatcgt cagcctgaat ttactcagat cgatgtggaa
     1321 acttctttca tgaccgcgcc gcaagtgcgt gaagtgatgg aagcgctggt gcgtcatttg
     1381 tggctggaag tgaagggcgt ggatctgggc gatttcccgg taatgacctt tgcggaagca
     1441 gaacgccgtt atggttctga taaaccggat ctgcgtaacc cgatggaact gaccgacgtt
     1501 gccgatctgc tgaaatctgt tgagtttgca gtatttgcgg ggccggcgaa cgatccgaaa
     1561 ggccgtgttg ccgctctacg tgttccgggt ggcgcatcgc tgacccgtaa gcagatcgac
     1621 gaatacggta acttcgttaa aatctacggc gcgaaaggtc tggcttacat caaagttaac
     1681 gaacgcgcga aaggtctgga aggtatcaac agcccggtag cgaagttcct taatgcagaa
     1741 atcatcgaag ccatcctgga tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt
     1801 gccgacaaca agaaaattgt tgccgacggg atgggcgcac tgctcctgaa agtgggtaaa
     1861 gaccttggtc tgaccgacga aagcaaatgg gcaccgctgt gggttatcga cttcccgatg
     1921 tttgaagacg acggtgaagg cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa
     1981 gatatgactg ctgcagaact gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat
     2041 atggtcatca atggttacga agtgggcggt ggttcagtac gtatccataa tggtgatatg
     2101 cagcagacgg tgtttggtat tctgggtatc aacgaagagg aacagcgcga gaaattcggc
     2161 ttcctgctcg acgccctgaa atacggtact ccgccgcacg caggtctggc attcggtctt
     2221 gaccgtctga ccatgctgct gactggcacc gacaatatcc gtgacgttat cgccttcccg
     2281 aaaaccacgg cggcagcgtg tctgatgact gaagcaccga gctttgctaa cccgactgca
     2341 ctggctgagc tgagcattca ggttgtgaag aaggctgaga ataactgata tgactcaaat
     2401 acacgaaatc attcgcgttg catcaaggcg gcaactgagt gaactcccat gagcatagat
     2461 aactatgtga atgggatgag cgaaggcagt caacgaagag gcagcgtgaa ggataaagtg
     2521 tataagcgtc ccgtttcgat cttagtggtc atctatgcac aagatacgaa acgggtgctg
     2581 atgttgcagc ggcgtgacga tcccgatttc tggcagtcgg taaccggcag cgtggaagag
     2641 ggtgaaaccg cgccgcaagc tgccatgcgc gaagtaaagg aagaggttac cattgatgtt
     2701 gtcgctgaac aactgacctt aattgactgt cagcgcacgg tagagtttga aattttttca
     2761 catttacgtc atcgctatgc gccgggcgtg acgcgtaata cggaatcatg gttctgtctt
     2821 gcgcttccgc acgagcggca gatcgttttc actgaacatc tggcttacaa gtggcttgat
     2881 gcgcctgctg cggcggcgct cactaagtcc tggagcaacc ggcaggcgat tgaacagttt
     2941 gtaattaacg ctgcctgaac aggcagggct atctggagat atttttatgg caggtcatag
     3001 taaatgggcc aacaccagac atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt
     3061 cactaaaatc attcgtgagc tggtaaccgc ggctaagctg ggcggtggcg atccggacgc
     3121 taacccgcgt ctgcgtgcgg cggtggataa agcactgtct aacaacatga cccgtgacac
     3181 actgaaccgc gcaattgcac gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat
     3241 catctacgaa ggttacggtc ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa
     3301 ccgcaaccgt accgttgctg aagtgcgtca cgcattcagc aaatgtggcg gtaacctcgg
     3361 cactgacggt tccgtagctt atctgttcag caaaaaaggc gtgatctcct tcgagaaagg
     3421 cgacgaagac accatcatgg aagcagctct ggaagcaggt gctgaagacg ttgtgacata
     3481 tgatgacggc gcgattgatg tctacactgc atgggaagaa atgggtaaag tgcgcgacgc
     3541 tctggaagcg gcaggtctga aagcagacag cgcggaagtt tccatgatcc cgtccaccaa
     3601 agctgatatg gatgcagaaa ccgcgccgaa actgatgcgt ctgatcgata tgctggaaga
     3661 ttgcgacgac gtgcaggaag tttaccataa cggtgaaatc tctgatgagg ttgcagcgac
     3721 tctctgatga tgcctgctaa acagcaaaac ggagacgcgt gatggctatt attctcggca
     3781 ttgacccggg ttcgcgcgtg accggctacg gcgtcatccg ccaggtaggt aggcaactgt
     3841 cctacctggg tagcggatgc atacgcacca aagtggatga tttaccgtct cgtctgaagc
     3901 tcatctatgc gggcgtgacg gaaatcatca cccagttcca gcctgattat ttcgccattg
     3961 aacaagtctt tatggcgaag aacgctgact cagccctgaa actgggccag gcgcgcggcg
     4021 tggcgattgt tgcggcggtg aatcaggaat tgccggtatt tgaatacgcg gcacgtcagg
     4081 taaagcaaac ggtggtaggt atgggtagtg ccgaaaaaag ccaggtgcag catatggtcc
     4141 gcaccttgct gaaactgccc gctaatccac aggcggatgc cgccgatgcg ctggcgattg
     4201 ccatcaccca ctgccacgtt agtcagaatg cgatgcagat gagcgaatcg cggctgaacc
     4261 tgacgagagg gcgactgcgt taagttatac cgcctcggtc agttccggct gaggcgtttc
     4321 aactccctcc gcgtgaatca acaccaaacc cggatgatgc cacaaactgg ctggcgtgac
     4381 ggttttacgc tcccacggaa tagaacctaa gaaccagaat ttccagaaag tgagttttgc
     4441 attcggattg ctatttaaca gttgtccaaa cgtctctatt tcacccaccg gaatacatag
     4501 cgcctcttta taaatatcaa agacaaattt tgaacagaac tggcgcgaag attcgtattt
     4561 aaaacccgtg tggtaaagtt tgcgtagccg ggagggaacc tgttcaacaa ttcgttgttt
     4621 ttgttgttct gtcagcccag cgtctaatcg ctttatagca tagcgttgat tagacgaacg
     4681 tttaataaaa cgagatagcg tggtgatggt tgaaagggga acccggcttt ctgcaaccag
     4741 gaagtcttca ccgttatgac cgataatgat cccgacgtga ttactccagc aatttgatgc
     4801 agctgatatt tgaccaaata aggcagcacc tatacatgta aagacgatat ctccaatttc
     4861 atattcagca gggtaattaa tattcacgtt gtcattccat tgaaatagat acgaatatac
     4921 gtaatagcat aaaaaattat ttttcaggag tcgcaatgta tatttttatt acgcatttct
     4981 tcactgaata tgtaatattc aaatatttgc ttccaatata acctgttgaa taaattatac
     5041 tgtgccattt ttcagatcat cgggacacct cgcaagtttt cttcatcctt cgctggatat
     5101 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
     5161 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
     5221 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
     5281 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
     5341 acggttttaa caataaacaa gaacgcacgt tgttcaaaga gttgatcaaa accaacggcg
     5401 tcggcccgaa gttggccctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
     5461 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
     5521 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
     5581 cgccagccgc cgacctggta ctcacgtctc ctgctagccc ggcgaccgac gatgctgaac
     5641 aagaagcagt tgccgcgctg gtggcgctag gctataaacc acaagaagca agccgcatgg
     5701 tgagcaaaat cgctcgccct gacaccagca gtgaaacatt aattcgcgaa gccctacgcg
     5761 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
     5821 gccggaagat gtagcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
     5881 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
     5941 cctcgatcat ttgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaacat
     6001 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     6061 gggcgatttg gctgcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     6121 gatccaccgt ttatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     6181 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     6241 gtttaccctg attggcgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgaccg
     6301 ttttggtatt gtgcaacgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     6361 tcgcagtgca cgctttatgg ggcttgagat gagtgatgac ggtgcgcagg aagtagctcg
     6421 tcgcgctcgc ggtacgccac gtattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     6481 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgc tggatatgtt
     6541 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     6601 taagttcttt ggtgggccgg taggtctgga taacctggcg gcagccattg gcgaagaacg
     6661 tgaaaccatt gaagatgtac tggaacctta tttgattcag caaggctttt tgcagcgtac
     6721 accgcgcggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     6781 aatgccgtaa gtcggattgg tcgctcgcgc cgcatccgac actggcagtg tgtctgatgc
     6841 gacactgttc gcgtcttatc aggccagcca gagatagcga accgtaggtc ggataaggcg
     6901 ctcgcgccgc atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat
     6961 actgataata aataacagtg ccgcacatag caccaccgac ggacccgccg gcgtatcgta
     7021 aaacgcggaa aaggttaaac cgccagttac tgccaccatc cccaccaaaa cagcgacacc
     7081 agccatctgt tccggcgtac gggcaaagcg acgcgcagta gctgcaggaa taatcagcaa
     7141 cgaagtaata atcaacgcac cgacaaattt catcgctaca ccaatcgtca atgccgtcac
     7201 cagcatcaac aacaatttca cttgctgtaa tttcacacca tcaacaaacg ccagatccgg
     7261 gctaatcgtc atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac
     7321 cacgccaatc gcaatagaga tgagatcttc tggcgtcact gccagtaaat caccgaacag
     7381 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacta ggcccagcga
     7441 cagggcactg tgcgccataa tccctaataa cgtgtcgatc gccagctgtg gacgcttctc
     7501 cagccatacc agaccgcccg ccagcagcag cgtaacggca atcaccgcat agaatggatt
     7561 cacgtccagc aacaaaccaa acgcgacacc aagtaatgag gcatgagcca gcgtatcacc
     7621 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     7681 catgatcccg gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga
     7741 cgcaaaacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     7801 agttgttcag caccacgagg accaaacatt gaaataaact ccggatgcag ggaaacaact
     7861 tccggtgtgc cggaacaaca aatgtggtga ttcaggcaca gcacttcatc ggtttttgcc
     7921 atcaccagat gcagatcgtg agaaaccatt aaaacgccac aatccagttc acgacgcaac
     7981 tggtcaataa ggtcatataa cgccacttgg ccatttacat ccacgccttg agtgggttca
     8041 tccagcacta ataattgcgg gcaatttaac aatgctcgcg ctaatagtac acgctgcgtt
     8101 tcgccaccag agagcttttg catcggtgcg ttaatcaggt gcccggcctg gacacgtttc
     8161 agtgcaggca aaatatcttc tttatgtgta ccagggcgta agcgtaaaaa acggtttacg
     8221 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgcagtttt
     8281 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     8341 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     8401 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     8461 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     8521 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     8581 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     8641 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg
     8701 aggttttact gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa
     8761 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc
     8821 aaaaaccggt aagcaaatta ccagaagcga agcaggtaac gattgcgcag cttgaagatg
     8881 tgaaaccgct actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     8941 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     9001 tagcgcgggc tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc
     9061 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attggcctca accgaaacgc
     9121 aggttggtaa cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     9181 acggctattt cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg
     9241 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     9301 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag
     9361 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     9421 caagctattc agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag
     9481 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccttg gcgtttaata
     9541 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     9601 ctgtctggcg gccctatgtt tatcaccgtg atgccacgcc aattgtcaaa accattgagc
     9661 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg
     9721 cacaagaaga tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg
     9781 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     9841 gtattgatat gggtgatatc acccaacttg ctgctgccga caaagaattg cgtaacctga
     9901 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct
     9961 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
    10021 tgaccagcga aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag
    10081 ggagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
    10141 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
    10201 tattaatgtc tcgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
    10261 gtttgcgttc agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg
    10321 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
    10381 gtatctcgtc taactttaac ccgcgtcgaa ctaatccggt gacaggtcgc gttgcaccac
    10441 acagaggtgt tgatttcgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
    10501 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
    10561 gcagctacac cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg
    10621 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaact gggccgcatc
    10681 tgcactatga agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc
    10741 gtaccgaagg gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg
    10801 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
    10861 gcttttttta tttggtgcgg ggcaagttgt gccgctacac tatcaccaga ttgatttttg
    10921 ccttatccga aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga
    10981 gtttgataaa tcctttcgcc acccgcgcta ctggggagca tggctgggtg tagcagcgat
    11041 ggcgggtatc gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg
    11101 ttttgccgga cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg
    11161 ctttccagaa cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc
    11221 gccgcaagcg atggcaatga tggctgagtt ggcaatacgc gggccggaga aaattcagcc
    11281 gcgcgttggc tggcaagggc tggagatcat cgaagagatg cggcgtaata acgagaaagt
    11341 tatctttctg gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca
    11401 agggcagaaa atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg
    11461 gaacacggtg cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc
    11521 attcatccag tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg
    11581 cccagagcac agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat
    11641 tggtcgtttg atgaaagtat gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg
    11701 caagacgcat cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga
    11761 tgatcatacg attgagcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc
    11821 agaacaatac acctggatac taaaattgct gaaaactcgc aaaccgggcg aaatccagcc
    11881 gtataagcgc aaagatcttt atcccatcaa ataaaaaagc ctctcgcgag gagaggcctt
    11941 cgcctgatga taagttcaag tttgcttcag aatattcgaa atctgttgaa ctatcattga
    12001 actataggcc ggatgtggcg ttttcgccgc atccggcaac gtacttactc taccgttaaa
    12061 atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccttgggt gacaatcacc
    12121 aggtcaccag acatcaagta acctttatcg cgcagcagat taaccgcttc gctggcagct
    12181 gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccacgata gagagcagtc
    12241 aggttcagcg tacgttcatg gcgcgacatg gcgaaaattg gtagaccaga gctgatacgg
    12301 gaggtcatca gcgcggtacg acccgattcg gtcatggtaa tgatcgccgt aacgcccttc
    12361 aggtggttag ctgcgtacat tgccgacatg gcaatagctt cttccacatt gtcgaactga
    12421 acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc caggcaaacg
    12481 cgcgccatgg ctgcaacagt ttctgacgga tactgcccag cggcagtttc tgcagacagc
    12541 atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc tgcacgcgtc
    12601 ggcatcgggt tagtaatcat tgactccatc atctgggtcg ccgtgattac cgctcggttt
    12661 agctgacgcg cacgacggat caacgctttc tgaatgccga ccagttccgg gtcgccaatt
    12721 tccacaccga ggtcgccacg tgcaaccatt accacgtcag aggcgaggat gatgtcatcc
    12781 attgcatcct ggctgcaaac ggcttccgca cgttcaacct tggcaacaat tttcgcatca
    12841 catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccaca gcgtgggaag
    12901 gagacagcca ggtaatctac gccaatcaac gccgcagtct taatgtctgc tttgtctttt
    12961 tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt attgttggag
    13021 aggggaccac cgacggtgac ttcggtgaac actttcatgc cctgaacttc cagtactttt
    13081 aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacgacgtc agcaggcagg
    13141 cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag gttggcgtcg
    13201 agcaggaatt tatccccaat attgaggaaa actttgcctt ctttaaaggt ggatacacgg
    13261 attttgggcc cctggaggtc acccagaata gccacatgac gccccagttt tgcggcaatc
    13321 tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga aaagttcata
    13381 cgtacaacgt tggcacccgc cgcgataact ttttcaagat tattatcgcg atctgttgct
    13441 gggcctaacg tggtaacgat ttttgttctg cgaagccttt tggacatgta atactccgtt
    13501 gactgaaaca accaggtgtt gcttgaacat gaaatccgaa tgaaataacg ccgcgatggc
    13561 gataaattaa tctcattcgg atgtcgtata agattaggac agtgacagtc gcttttagcg
    13621 atcgtcactt aaattaagta actgcttatc aaaacgcgat tccttcagcg cttccttgac
    13681 ccgcttcaag ttatctctga attttgcacc gcgtcgcaaa gtaaatcctg tcgccagcac
    13741 atctatcacg gtcagctgtg caagtcgaga aaccatgggc atataaatgt cagtatcttc
    13801 cggtacgtcg agggtaattg ccagcgttgc ttcccgggcg agcggggtac ccgcagaggt
    13861 gagggcaatc accatggcgt cgttttcgcg tgccagctgc gccagctcga ccagattttt
    13921 tgttcttcct gtgtgagaaa tcagcaccac cacgtctccg tcgctacaat tcatacaact
    13981 catgcgttgc agcacgatat catcggagta caccaccgga acattaaaac gaaagaactt
    14041 attcatcgca tcgtgggcaa cggcggcaga agagcctaat ccgaaaaagg cgattttttt
    14101 tgcctgagtg agcaagtcga cggcgcggtt gatggcagat ttatccagtg aatgacggac
    14161 atgatcgagc gttgccattg cggactcaaa tattttcccc gtgtatgatt caacgctgtc
    14221 atcttcattg acgttgcgat taacataggg agtgccattc gccagacttt gtgccagatg
    14281 aagtttaaaa tcaggaaaac cgcgcgtgtc catgctgcga cagaaacgat tcaccgtcgg
    14341 ttcgctaaca ttggcttcca gcgccagagc agcaatactc gaatggatcg cgttatcggg
    14401 cgaagccaga atgacctcgg caactttgcg ctctgatttg ctcaaatgtt ccagctgaga
    14461 ctggattttt tccagcatat tcatgatgta aagagactca cgggtaatga cgatttccgc
    14521 actgaaagaa atcgaaatgc agttttgtca gatattacgc ctgtgtgccg tgttaatgac
    14581 aaaagcagat aaaaaagttg ttattttttt cataacatga tcagtgtcag atttttaccg
    14641 aatggaaaac gatgattttt ttatcagttt tgccgcactt tgcgcgcttt tcccgtaatc
    14701 gcacgggtgg ataagcgttt acagttttcg caagctcgta aaagcagtac agtgcaccgt
    14761 aagaaaatta caagtatacc ctggcttaag taccgggtta gttaacttaa ggagaatgac
    14821 atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac
    14881 cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagtcgg tcagctcaac
    14941 ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa
    15001 gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac
    15061 accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcaac
    15121 cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacta ttaactactt tgccatgccg
    15181 cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg
    15241 gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat
    15301 gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt
    15361 aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac
    15421 tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa
    15481 gggcgctggg gctattttga taaagccggt cagatgcgtg acatgattca gaaccacctg
    15541 ctacaaattc tctgcatgat tgcgatgtct ccgccatctg acctgagcgc agacagcatc
    15601 cgcgatgaaa aagtgaaagt actgaagtcc ctgcgccgca tcgaccgctc caacgtacgc
    15661 gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga
    15721 tatctggaag aagagggcgc gaacaaaagc agcaatacag aaaccttcgt ggcgatccgc
    15781 gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt
    15841 ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt
    15901 aaagagtcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa
    15961 ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa
    16021 atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc
    16081 tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac
    16141 gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat
    16201 gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt
    16261 acccgtgatg gtcgttcctg gaatgagttt gagtaatatc agcgcttatc ctttatggtt
    16321 attttaccgg taacatgatc ttgcacagat tgtagaacaa tttttacact ttcaggcctc
    16381 gtgcggattc acccacgagg ctttttttat tacactgact gaaacgtttt tgccctatga
    16441 gctccggtta caggcgtttc actcataaat cctctgaatg aaacgcgttg tgaatcatcc
    16501 tgctctgaca actcaatttc aggagccttt atgaatccac aattgttacg cgtaacaaat
    16561 cgaatcattg aacgttcgcg cgagactcgc tctgcttatc tcgcccggat agaacaagcg
    16621 aaaacgtcga ccgttcatcg ttcgcagttg gcatgcggta acctggcaca cggtttcgct
    16681 gcctgccagc cagaagacaa agcctctttg aaaagcatgt tgcgtaacaa tatcgccatc
    16741 atcacctcct ataacgacat gctctccgcg caccagcctt atgaacacta tccagaaatc
    16801 attcgtaaag ccctgcatga agcgaatgcg gttggtcagg ttgcgggcgg tgttccggcg
    16861 atgtgtgatg gtgtcaccca ggggcaggat ggaatggaat tgtcgctgct aagccgcgaa
    16921 gtgatagcga tgtctgcggc ggtggggctg tcccataaca tgtttgatgg tgctctgttc
    16981 ctcggtgtgt gcgacaagat tgtcccgggt ctgacgatgg cagccctgtc
//
LOCUS       DAJTNV010000082        16438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNV010000082 DAJTNV010000000
VERSION     DAJTNV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            62..1063
                     /locus_tag="OO199_002905"
     CDS             62..1063
                     /locus_tag="OO199_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5536874.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1354..1788)
                     /locus_tag="OO199_002906"
     CDS             complement(1354..1788)
                     /locus_tag="OO199_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5536875.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1760..2551)
                     /locus_tag="OO199_002907"
     CDS             complement(1760..2551)
                     /locus_tag="OO199_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5536876.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2551..3231)
                     /locus_tag="OO199_002908"
     CDS             complement(2551..3231)
                     /locus_tag="OO199_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5536877.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3228..4427)
                     /locus_tag="OO199_002909"
     CDS             complement(3228..4427)
                     /locus_tag="OO199_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5536878.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4428..4781)
                     /locus_tag="OO199_002910"
     CDS             complement(4428..4781)
                     /locus_tag="OO199_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536879.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4781..5521)
                     /locus_tag="OO199_002911"
     CDS             complement(4781..5521)
                     /locus_tag="OO199_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5536880.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5744..6034
                     /locus_tag="OO199_002912"
     CDS             5744..6034
                     /locus_tag="OO199_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCR5536881.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6058..6402)
                     /locus_tag="OO199_002913"
     CDS             complement(6058..6402)
                     /locus_tag="OO199_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536882.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6415..7479)
                     /locus_tag="OO199_002914"
     CDS             complement(6415..7479)
                     /locus_tag="OO199_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536883.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7482..7787)
                     /locus_tag="OO199_002915"
     CDS             complement(7482..7787)
                     /locus_tag="OO199_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536884.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7789..8391)
                     /locus_tag="OO199_002916"
     CDS             complement(7789..8391)
                     /locus_tag="OO199_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536885.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8391..10406)
                     /locus_tag="OO199_002917"
     CDS             complement(8391..10406)
                     /locus_tag="OO199_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR5536886.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10584..11009)
                     /locus_tag="OO199_002918"
     CDS             complement(10584..11009)
                     /locus_tag="OO199_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536887.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11013..11453)
                     /locus_tag="OO199_002919"
     CDS             complement(11013..11453)
                     /locus_tag="OO199_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCR5536888.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11464..12609)
                     /locus_tag="OO199_002920"
     CDS             complement(11464..12609)
                     /locus_tag="OO199_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR5536889.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12613..13170)
                     /locus_tag="OO199_002921"
     CDS             complement(12613..13170)
                     /locus_tag="OO199_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536890.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13145..13534)
                     /locus_tag="OO199_002922"
     CDS             complement(13145..13534)
                     /locus_tag="OO199_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCR5536891.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13521..14075)
                     /locus_tag="OO199_002923"
     CDS             complement(13521..14075)
                     /locus_tag="OO199_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536892.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14072..14479)
                     /locus_tag="OO199_002924"
     CDS             complement(14072..14479)
                     /locus_tag="OO199_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR5536893.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14478..14712
                     /locus_tag="OO199_002925"
                     /pseudo
     CDS             14478..14712
                     /locus_tag="OO199_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14709..15650)
                     /locus_tag="OO199_002926"
     CDS             complement(14709..15650)
                     /locus_tag="OO199_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5536894.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15662..16165)
                     /locus_tag="OO199_002927"
     CDS             complement(15662..16165)
                     /locus_tag="OO199_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536895.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16170..>16438)
                     /locus_tag="OO199_002928"
     CDS             complement(16170..>16438)
                     /locus_tag="OO199_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536896.1"
                     /translation="SRKMKPTVFKREVLASADKTLVRQVVGDADIRKLPKQSVDMAFN
                     AVSEIAKGRNTRSTTGDAQRPNMGMTSIASLNKQNAEFWSNRKG"
BASE COUNT         3841 a         4415 c         4037 g         4145 t
ORIGIN      
        1 aaatggacta tgacataaag atctgatatt ttcctcacaa aaacttaggg attttatttt
       61 aatgcataag agcaactgct ttgatacggc aaggcttgtg gcagctatga tggttctaat
      121 cagtcaccac tacgcgctct ctggacaact cgagccgtat atattcggct ttgaaagtgc
      181 tggcggcatt gctgttatca tattcttttc catatcagga tacctgattt caaaatcagc
      241 aatcagaagt gattccttta ttgatttcat ggctaaaaga gcaagaagaa tattccctgc
      301 tcttgtccct tgctctgtac tgacgtattt tttatttggg tggatgctta acaacttcag
      361 tacagaatat tttagtcatg acattatccg gaagaccata agctctattt taatgagtca
      421 ggctccagat gccgacatta catcacattt aattcatccc ggtattaacg gcagcttgtg
      481 gacactacca ctggagttta tgtgttacat aataactgga gtggctgttg ctctactaaa
      541 gagcggaaaa acgttcatag ttatactatt gatttttgtt tcactttcat tagttgaaag
      601 tgtatctgag aacaaggatg taatattctc cattccagta tggctgtatc ctttgcgggg
      661 gatggctttc ttttttggtg ccactatggc gatgtatgag aaatcttgga atgtaagtaa
      721 tgtgaaaatt tccgtgattt ctttgcttgc tatgtacgca tacgcgagct atggcaaagg
      781 cattgactat acaatggctt gctatattct ggtttctttc tcaacaatag ctatatgcac
      841 atcctttgga gatccactgg ttaagggtag gtttgattac tcttatggtg tatacattta
      901 tgcattcccc gttcagcagg ttattataaa tacattgcat attggctttt acccttcgat
      961 gttgttaagt gccattgtcg ttcttttctt ggctcatctt tcttggaatc taattgaaaa
     1021 aagattcctt acacgaagct cgccaaaatt atccctgaat tgaataataa aggggccatc
     1081 gagcccctga ataaatcaaa gccagatata acactgcctg gcagtggaca ccgttctgat
     1141 gcctcgtcct gcaaggacga gcgaaggtgg agttcttttc cacgcagcta tttagtacag
     1201 tactgagtca tattgtttat gtatgagtta acttccgtaa tatcaattaa ataaaataag
     1261 ttgaggccat tgtagaagtg agcattatat ccaggttatc tggacctgtt gaaaaatgcg
     1321 tattattcca acaattaccc tagattaaca agtctattta tttggatgca taggccactc
     1381 gggttttact acattaacac gcataagcag tacgcgatat tttttccatg ccagtaacag
     1441 tttcgtttca tcatctgttg ccatacctaa atcaaccgca tcctgaagcg gcgctattac
     1501 attcgttgcc tctgctatca gtgcagattt cttttcatca tttattaagg ctaattgttc
     1561 tggtgttgga gaaggacggt caacaatgac agggtgaccg ttctcaccac aggtaatcat
     1621 ttttgttaca ctctgtgagt taatcagtgt tttccactct gattccgaga tttccactgc
     1681 atcatcagga atattggtgc cgtgaaattc agtcgaataa aatccgtttg tcgatgcaga
     1741 ataaaaatat gacatttcgt taatatccca ttgcaatgta atacgcatca gcagatgaac
     1801 catatcctga accgggcgac actgtaatat caaatccagt gttatggaca tttgtcgccg
     1861 atacagtcgg ctgccctgac gcgccagtaa cgttcaatgt aactgtgaca gcaaggactc
     1921 ttgagaatgt catcgggaac ctcatcccgt atgttgaaga tacattcggg gagccattca
     1981 actgaccgca ctgtaaaatt aatccagacg gaaatttttg cgcagcagcc gttgcggtat
     2041 tacttgacgc gaacaggctc atatcaggta tctgattcgc ccctgtacct acattccttt
     2101 tagccgcttc tcccaaacca aggtatgtga gaatgtcagc aatagtattt ttcccaataa
     2161 tgtcacggcc aacagaagtt aaatcagtct gcgctgctgt atcatttcca gtgaaatatg
     2221 ggagtttatt tgcacctgtt gcgagcccag ctaatgccga cagcgtggca tcaagcgcct
     2281 ggaaatcttt cccgaaagcg gtccccattt tggatataaa cccgttcagg tctccatcat
     2341 caagcacatc cagcctgctt ttgttggcgg tgtactgcgc caacgctgcc gcgataaagc
     2401 tggcttgccg aatagctttg ttgacctgtg cgctggatgc tttacctgcc gtaaaccctg
     2461 aaagcagagc cggaagtgct tcccagtcag cttgtgaggt gacgttagcg ttcggatcaa
     2521 gcgcgaaagg tttaaagttg tttattgcca ttagagtatt gtcccccatg ctccaacatc
     2581 gaacccgccg atgtattcgt tatccatatc aaacccaaag aatttagagc cctcggatgg
     2641 tgcttctacc gaaggcgttt caacatcacc ggcccatacg ccagctgctt taacggtgag
     2701 atagccctgt ttgatagcgg caatcagttc aagagacaca tcagaaatat cagtctcggg
     2761 gaatacccaa accgatatcg tcatgtcctg gttgtcgaca atttgcatcc tcaggcctga
     2821 gccttcagtc gcagcgtcaa ggatgggagg cagagagtcg ttccgaccgt cccagttgtt
     2881 gatagcgatt ttcgctttca gaatgatgcg gtacgtctca tcgcttagcg tcgtatagcc
     2941 agaatcagga tcatatggcc ctttccagat gccctggtca tacccaagcc cgtcagtgtc
     3001 ccagctgaaa taaactccgc taattggctg gctgactatg cgactgcgtc cgatccacag
     3061 accgaggatg tcgagctgta caccgacagc agtatcaata tcgaaggctg ttataagccc
     3121 tgacatagtg ctggacacat caatcagcgg gcgggtgctc agatctatat ggtcaaaaaa
     3181 gagtggcttg gtagcgtggt agttagtgat cagttcggtg tatttgctca tgaggtcacc
     3241 gtgatactga tattcgcggt gctacaggac gcagaagcat cataggcaat atcaatgttt
     3301 gatgccgata cgctgccaga cgacttaccg atcagcaggt cggtaatatc gtaatagcgg
     3361 gcattcccgc cgctcacaac gccgaggttt gccggggaat aaatacggct cagcagaacg
     3421 tcgtcgccaa ttgttaggcc atttatataa tcggcaacag cctgtttaat ctgctcgccg
     3481 atttgagagg tatagccggt aaaaactttc agggtaatgg ctacgaaaat tggcacatcg
     3541 gtagagcgcg aaaaactgat gacgtgtgga ttaccgtaag tatccggcac cgtgacagaa
     3601 gttttaccgt aagttgcggt tccctgccct ttactccccc ggattgtttg ggctatctcg
     3661 gtaacatccc ctccatcgac gatggcggaa atagagtgtg gcggcagccc gttgctgtcg
     3721 gttgccccag tgtcgttctc atatagtttg tgacgtgtca cgccagcaac attagcgata
     3781 gcaccgtcga caccttcaaa cggtgtgatc gatggtagcg cgacgctttg cccctgccga
     3841 atgcgcagct ctgcgtcggt ttcggctggt gaaccgacag tagccgcaac tggattggtt
     3901 accgacaccc aacctcgggt cggggtgttg atagtggtaa tagtcccggc cagcgccgca
     3961 accgaaccgc tattcgcaca tgtggccgtc accagcacag taccatcaac gccgatcgct
     4021 acacttgcgg gaaaattcca gataatgccg tttttatccc gtgcggagcc attcgtgata
     4081 gtcgtgcctg ccgtaccggt taacagaagg tcagcagtag agtttgtcgc tacttttcgc
     4141 gtgatcccgt taattttcac attgctgcta agcgctgcgg cctgcgctgt cgtcggtgaa
     4201 aaagagttgt agatcccgat agcggtgttg ttagcgtcat gcacggcaag agccaccagc
     4261 gcgaccatct gcccgtcttt gctgtctggt tcgaggtagg catcactacc gtaaatctgc
     4321 ctgaaatagc tggtcagtgt atctaggatt gtctggtaat caggcgcact aatcccctgg
     4381 gcggttaccg ttgccgatag ccccagcgtg tcgaggttca aagccattta tgcctcgctt
     4441 gttacagtcg tctggccgta gattgtgtca atggaggaag tgaaggtgac gcgacggctg
     4501 gtgccgtcat aattggtatc gaaggaaaga atcgacagaa cgcccggtgt atcctgtatg
     4561 cgttcgcgta tagccaggat gtagacatct gatcgctgct tcccaagcac tgactgaaca
     4621 tacggcgtgc cttccgtcag atcgagaaac cactgaccgc gccacagctc gaaacgggtt
     4681 tttacggcct gggcgacaca ttccggactg tcgataagga aagtatcgtc accctggccg
     4741 aaagtgtaat cgccttcagt atcttcgcga cggtatcgca ttattgcggc cctccggtag
     4801 ttcccccgcc agtctgtact ccgccatgtt tatgcgtggc gacactgata ccagaagctg
     4861 ttacgtcatt cgttaccatc accggcccaa gcatcgtcgc agtaccgccg ctttcgccca
     4921 ttccctgaga caagttgcca ttaatcgtta cgttgccgtt cagcgtgata gtcggggatg
     4981 tgattgtcgt tccaccttca gccgtagctg taagctggcc cggtgtttta atggtgatgt
     5041 tatgtcctgc ggcgacttct acaaacgccg caccatcatc ggttcgcagc tgcgcggcgc
     5101 tggtactgat accgctgatt ttccgtgctt gcgactgcgg gccaacgatg gcgaacgcat
     5161 cagataagtc atgctggcgc gggtcgacgg tctcctgaac gccgccgctc tgccaccaaa
     5221 aatcgatgca acggtcagca aaaatcagga ggcactcgtc gccttctttt accggaaagg
     5281 tcagcgtgca accgccgccg cgcgggaaga tgaccggcac atccaccagc ggttttaatt
     5341 cggtggatcc atcgccaaca ataccgcgta gcgccacctc tactgtgcag gtaacagtct
     5401 caggatcgaa cgactgaatg atgccgggca tcgctacgcg catctgggta gacaccgaat
     5461 cggcaatggc ctgcgcggtc tgctgctcac cgccgatctg tgattgagtt ggaattggca
     5521 taaaaccccc ataaaaaaac cagccgaagc tggtttggta tcaattatct cttagtaact
     5581 aagctacatc agtgtgaagt aaggcttttc cgctgcaatg gcacacattc acaaggtgta
     5641 ttaggatatg tttgcaacaa gtaagcattg aaaatcaatt tcagccatga agccgccata
     5701 gagtggacct gcaccatcgc attattgact acaggagtaa caaatgggat ttagatttcg
     5761 taaaagaatc cggattgcgc ccggactcgc gatcaacatt agtaaaagcg gagtaagcac
     5821 ttcaattggt ggtaaaggtt ccaccattaa catcgggaaa aaaggcgtaa agatgacaaa
     5881 tggccttccc ggcacggggc tgtcgcatac cactaacctt tactctcccg ggaaatcgac
     5941 agaaaaaaaa caacttactc ataagcaaaa aataattaga aacatcctgt tcgtaattat
     6001 tgtgtttata ataattaaag ctcaatattt ttgacgcatg cccgcctatc tggcgggcta
     6061 ttttactttt cggcagtcgt atgttgcata ctgacgcggc gaatccatgc tggcttgcag
     6121 ccactgtgcg ttgagaatgg ctttgccgtt tcgattgatg tactcaagac caacccatct
     6181 tccaggctga tcggtagcca tacgccaatc catcttgata ttgttataat cgcctttgtt
     6241 tttcaggaag gtgatttttt gcatttctgg ctttgcgcca ttgattctgg ctaagccatc
     6301 atctgcccaa tggattttaa aatcaccaca ttgctgatcc gcaaaagcgg gtgctgatat
     6361 aagcaccgcg aacacggtaa cagtgcaaag taaatgtctc acggccccac ctcactaatt
     6421 cgtcgtccct tgcattgctt tcgggctata aagatcacga gcgccacgcg caaaacacat
     6481 caaatccatg taccacgcct gacctctggt gtcgccagta tagtcgatag ctttgacgat
     6541 ataaacgcca tccgtcgcaa tgctggcagc ctgtgacgtc gtgccggtca gcacacggtt
     6601 gccgttctct tctgtttcgg tgatacgccc gggcgactgt gctatttcgc tattgccgag
     6661 cgtggcgcgg tacaccgaag cctgatcgag ctggataaga ccattaatac ggatgtttgg
     6721 gtttatcagg catcgcacgt ttactccgcc acccatcgtt tgttgcggca taccgatcag
     6781 gccagtattg gcattcaaca caatggcttc gtgaatatat ttatcctccg gcaccatctg
     6841 gacctgacca tccaccagtt gccatgtcgc tttgcactgc gcagcaatat tatccatcac
     6901 gttgcggctg gatgagtaaa tcgcgcggcc acgaggaaac acggtatcag gaaaatcgcc
     6961 ggtaatgccc tgtgtcacgc cgaacgcgtt gaaatcctgc atcgtcgccc ggtgcagatc
     7021 cgcaacggta tatccagcgg caagcgtggt gatggtggtc gcatagagga acgcttcgtg
     7081 atcaccaatg gcctgaatca acacccagga atccgtaatg ttgtccttcc cggtgacggt
     7141 gaagcgaata tcaccgtcaa aaatcaggcc gtagttctga ccgttcacct gccctatctg
     7201 gtctggtgaa atctcccggg acacaccaac ctggctcgca tcaacatccg gcgctatacc
     7261 gtcatacccg gcaatgatgc gaattttggc aaactcctgc ccaagtatct tgttcgtggt
     7321 atcggtcgaa aggttgtaaa ttttcacgtt cgccacgcgc ggccagcgtg tgtctgccca
     7381 ctcgatctgg aacgtgacct taaagtcaga cagggaaacg ccctgcccgt tctggtccaa
     7441 cagttgcagc tcaaaatggc gcatccagtt aagagacatt tctactcctg tacgaaaatg
     7501 aggtggctgt atgtgccgag gttggttttg gtgggctcgt ctggtgcgcc cttatcggtc
     7561 gccaccacca gcgcgccatc aatgccaagc tgtggatatt gtcgtaaaag gtttacgccg
     7621 gtcagtagag gtacgccaga gagaagcgcg gcaccgccgc tatccatcac gtccataatc
     7681 cagccagccg catcacgcca gatgatcctg agtgtatacg tggtattgcc cagcaaaact
     7741 cggaactgct gattgtcagg agaaagcggt atttcgttaa actgcatatc atcccccgaa
     7801 tgctgatgta acgcttccgc ccagctggct cagcaaggat tcgtttggcg gtgtagtgga
     7861 tttcatcccg gaattctgca ccgccgatgt gctgacgcca tcctgcatat ctgatttatc
     7921 tgcaacgcta acgctctgcg tctgcgacat gatcacttca cgcagggtaa gcgtgcagtt
     7981 cagcacgttc tcgctggttt tatccgttgt cacctcgatg gctcgcacca gcatattgct
     8041 gtaaacccgc tttccggtca ctacatcgaa cggcacccta gaggactgga gatccagtag
     8101 ttgctgatag gtctctttcg ggctaagtcc ggcgctgagg ccgattgacg atgtatcaat
     8161 gaagtccagc aacgaaccgc cacctgcgaa gccgcattcc atcgtgactt cgctggggcg
     8221 cttatacgca tgatcggcga tgaaacccga cgcgctattc gttgttggct tctccaccgg
     8281 gtgctcagta atttcgagcg catcagaatg cttttcagaa acaaccacgc tggggattaa
     8341 tattccaatt cgccgggatt gctggcgaaa aattgctgat aaaatatcca ttatctcggt
     8401 cctgcgggga gttgctgggt taactgtgaa ttcacgccct tttgacggtc aacagtcaaa
     8461 cgggcagcct cgcgcggatc gaaaacgccg tggatgttaa tattcgtttc ctgctgaatc
     8521 accggggcgc tggtgggcat attgctcatt actttcggaa tgtagttgcg cgtttcctgc
     8581 ggcattagcc ccattccata acgcttaaca ttcccgatcc cccagttata tgatgccagt
     8641 gctttgctaa ggtctccgcc gttctgccgc aacagctggc tcaggtactt agcggctgcc
     8701 tgagctgact tttccgggtc gaatacatca ttcccacgca ggcccatgtc gcgcgccgtg
     8761 ccatccataa actgaaacag acctttagcg ccagcgcctg aaacggcgaa ctgattaccg
     8821 cccgattccg ttatggccac gctgcgcaat aacccctccg gaagccggta gagctgttcc
     8881 aggttggtaa acatcggctg catccatccc agcagttcag agcctgcttt ggttggctgt
     8941 ggccgcttaa ctgactggcc gagctgctca ggctcatcat caccgaacca gccgcgcacc
     9001 gttcggccca cgctgcgagg atcgaatccc cagtgctctt taatccagtc ggcggttccg
     9061 ttggcgctgt ctgttaccat cggcatcgct gacggatttt cgctgccctg attaagcatc
     9121 tgtttgccga tgctggcggc atcagcccag cggccatctt taatggcgtt gagcaggtcg
     9181 gcgatcatgt tcagcatctt gccgaactca cccatttgcg aaatgaagtt gctaaaatcc
     9241 cactttaggg accatgactt ggggtcaata ccgagcaact tagccagcgc ttttgtcagg
     9301 tccgtaacag tctttttcag gtcaccaatc attttgacgg cctgatcaat ttcagtcttc
     9361 catttccccc agtcaatcag gcttttgcca ccttctttcc acgtctggta atcatccaag
     9421 agcagaccaa gtgcaataat aagcgtcgtt atcatgccca agggtgatga catgaaagcg
     9481 gtatttagca gccgccatgc aaccaacagc ccactgaaca atgcgataag ctgctgcgtg
     9541 gctgggttca gctttttgaa ccaggcgatc acaccctcaa cagcttgccc agttcgccat
     9601 aacacgcggg taatcgcgtc acctgcccag agaattcctt tgatgatctt cgtgatgacc
     9661 gcttcaatct tcggccagtt gtcgagtatc tgcctgcgga agttatcaat actgccagca
     9721 aggccaccag ctaggttaga gccaatcttg tctttcgcct gtccaagcgt catcgtcaga
     9781 ttacgcatgg aggtcatgaa aatattggac tgtttagccg ctgactcagc attaaaacca
     9841 atacgctttg ccgtcaacgc gtactcagaa ctgagctgcc ccatccctct gcgcatcgcc
     9901 atcagcgtgt tttcatcgat gccgagcatc tgcgcgtatt gcttcgcgcg gtaatacggc
     9961 atgttgttga gcttttggcc aacgccagta aagatggcag cagtatcacg catctttccg
    10021 ctggcatcgc gggtctggac accaagacgg ttcaggaacc cttccgcccc cggattgcta
    10081 cgcatgaagc cagccaaccc ttcgagggag gacatagccg actcggcgct ggcaccggtt
    10141 tgcgatgcgg cgtatcccag cgctttgatg ccctggacac tggcccccgt ccgctgggat
    10201 gcccagtaaa ttttatccag accgttcgcg atctgagtgg taaatccgac aatgctcagc
    10261 gctgcgcctt tcaccaccgc gccgaccttc agaacgttcg cggtaacgcc tttcagcacg
    10321 gcttcaaact tattagcgcc agcctgatcg atatcgaatc ccagcgaaac aaggaaatct
    10381 ttaatcgtat ctgcgttacc gctcattggc cgctctccat ttatctaccc gggcgtcgtt
    10441 atcctcgcgc atgtcgaggt agtcattgag aagcgcgatg cggcaaagat ctaccgcacc
    10501 gctgttaagg tctttctggt caatatggaa ggcaagcgcc gggcgaagaa taaaatcttc
    10561 accgcccggc aggctgttga aggttattcc gctagcgggg tgggcgtctc gctggtaggg
    10621 agtccttgca aaaaatttcc cagcgagtcg gcgaccaccc gcgccaccag ttgcagcatg
    10681 gtaagcaggt cgatatcgtc aaacgccatt tcgccatgct ggcagaccgg cacccagcct
    10741 ttcatgtgct cgcgtgaaac aacggaaaga caggggaaca ggatagcgtc cacgtcgcca
    10801 tcactcagat cggacacagc attggcaatc tttggcagga tagtagccat cgcgccttcg
    10861 gtgtctttgc tgctgatctt ctcctgaacg ctccggaagt ccgaaaccat cccggccaga
    10921 accggcaaca gcttgcggga caccttcagc tgttcgaaaa cgctgagctt tgcggtgcga
    10981 tatttcacgc ctttaatttc gaattccatg cgttaaaact ccccgagaag ctggtcaatc
    11041 ttgccgcagt cgaataccca ggcgacggtt ccgccctctt tagcgttatt gaaatcaggc
    11101 tgtttctgga atgcacacga acgcgcagta gaaatatcac ccgatgccgt gttgcgaatg
    11161 acgatcacgt tattgcccca ggtggcagag gactggcttt gcgcgttata cgccagagac
    11221 agctttttat tcaccgggga ggttttgagt agcgtcaccg taatggtgcc tgacttatcg
    11281 gcgtgcaggc tgtgcatcac ctcgccatcg gcaccgatag tcatggtgtt tttgttgccg
    11341 cccatggtct gggtgatacc ttcctcagag ttcgcagaac cctgaccaag atcgataacg
    11401 ccggtcggcc cggtgagcga cgcggttaca tcgagaaaag aataagttgc catttatcgc
    11461 tccttagcga accacgttga tctgcacatc ggcataatga actgcgccag ccagcttaca
    11521 ggccacctgg attaacggtg ctttgcgcgc ttcacggtcg gcctgcgcct gttcggacag
    11581 cggttgcgca tacacgtaat agcctttggt cagcgtatcg ccggaattca gctgtccgat
    11641 agggccacca ttccacacgc cagcagctac cagaccgttc gtgacggact gatccatgga
    11701 ctgttcaacg ttggaaagca gacgggtcac accggcatca gtctgcggaa ttttggtggt
    11761 gctggtgtaa agcaggttat agaggttggt ctgaacgtag ttctgcaacc agtcgagccc
    11821 gtggcgctcg tcgaagaagt caccgttcgc catgacaccc tgttgcagga tcgccgtgtc
    11881 gttggcgtag tacacgaata cattgcagtt ttttgcatca agtgccgatg cctggctgac
    11941 tgtcagtgtt tcatacccga cacccggctc ctgcttaaac ttgagcgtaa tcgcggtatt
    12001 actgccattg aaattaaccg tgaatgcccg gccaaatgca gataacgcag cgtatttatt
    12061 acccgatgaa tattgaataa aactgcgtga atatccggcg gttttcagtt ttgatgccaa
    12121 atcatcgctg gatgcagtct gcaggcattt ctcatcgctt gtcgtaatcg ccagaatacg
    12181 gcttacagaa gaggattcga tcgccgcagc cactttcagc cagtctgcat ccggaatatc
    12241 ttcatcgtct gcaatcccca gcccatacca tgaagtataa tcgagcatgg cattcacagc
    12301 ctgctccagc gtctcaggcg tggcctgttc gctgtctccc ttcgttttca cccaacgacc
    12361 aacaaaaacc tcctgaggtt tcggtgattg tgagaaaaac acctgcgcag ccttatattc
    12421 tggtgattcc acgccaaaat cttttccaat atcttccgcg gcagaataac ggcgaatgcg
    12481 ctcacttacc ggaatgattg tggacgggcc gagaatgagt aatgcaccaa aatttcgccc
    12541 tgatgctgca cgcggcgaca tgatcacatc aacattaaca acgtttgata caggcaagcc
    12601 ctgtgccata gcttaatctc cgaaaaagat gactggtgct tccaccagcg atttaatacc
    12661 gtactcgcgc acaaccttcc ggcgcagacg caccgtcata tcgtagcggc ggacccattg
    12721 ctgattaata agttcaggga agagacctgt gtaatcgcca agagacagcc ccagcgcatt
    12781 cagtgctgcg ttgttctgcg gtacagatat accgtcacga aaccgggacg catacaccat
    12841 ccccgccgga ccataaaacg aagccataca ctcaatcgtt tcatgccgcc agagctgaga
    12901 gccatcatcg gtctgtctgg tgaatgccgg actgtcatca cctgaccatc cgataacccc
    12961 aaacgcacac cagttcgttt caaccggtag cagtggcggc tgctctttct gccagcgcgg
    13021 gcgaaccatc ccggcagaca gaccggaaac gttacgcatc cactggctta acagcctgtc
    13081 gagcgcttcg tcataatccg gatcgccact ggttggtatt aaccatccgc gctctgtact
    13141 ggtgttattg ctcaaccgga gttcccccat caaacggcat caactcacaa tgcgcctgaa
    13201 cgaatccggc cccataagct gtatacgggt cgacgaaggt cacacgataa tcacggccct
    13261 gatacgtcac gatatcggca tcacggccag tctgtccctg cgtcagtcgc tcagtcgtca
    13321 caatcagaat tgcaccgctg attacctgcc ctgcctgcat acggcggttt tccagagagc
    13381 gatcaacagt tacgactccg gcaaactgct ttttaacttc actgtcgctg ccgatcccgt
    13441 cctcatccac cgtttgcact cggcgtgtta cccacaaatt gaagtcgcaa aaatcggggt
    13501 caaaaagcac atctgttaca tcaagagtcg gcatctttat ccctcacaac atgggtaata
    13561 gctctgcgat attgcccggt gtcaattaat ggtttcgcca gatcggttcc gggagattcg
    13621 ccagcaacac gccgagcaag ttccagtgtt gcccccttgc gcccccgacg agcccgggct
    13681 tcaacggtgc tatcagcaag cggtgtaaag ccggtaatgg tcatgtaacg cctgacgcca
    13741 ttcgcggcca gcgttccggc gcggttaagc gctctttccg cacccgccgc atttccatca
    13801 agcgcagcct gcgccgctgc tttgagctgc ggcaccgtct gttcctctac ggatttaacg
    13861 ccggggatca ggtgcgggcg tgggggtatg ttttgcgctg gtgagccgta ttcgttgacg
    13921 taaccgatcc cggcattacc aaacggaaca tcctcacgct cgctgtcttc ttccgggatg
    13981 cccaccagca catccttttt ggttagcgac cggagcgcat ccagaatggc ctgagcgtta
    14041 tcaaccctcg ttgttacacc acttttgaaa ctcatagctg gcgaccgccc gcaccgaaca
    14101 tcgtgatcag ctgataaaat tcagcgccat atcgggtgtt attccagaag cctgcgtcag
    14161 ggtttagcgt cgcgctggtg tcatagctga cgcttacctt gtcaacggac ttggaggact
    14221 gaacaccatt ggttgaaccg cccgggccgc caaccagcat tgcccgacta tctgccgccc
    14281 atagcgtcat gtagtgagcc acgaacaacc cggcaaagta cggaaacaaa tctttgccgg
    14341 tgacgttttc gctcagcagt tcatcggcca gattcagacg aaactggatt tgcgcttcgg
    14401 gatatttggc agggtcagca aactgcggga agtcgcggcg aaaatcactt accgctggca
    14461 ggctttgatt ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat
    14521 acttccgcgt gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac tgcatgacgg
    14581 cctttaacaa actcgcgccg cgaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg
    14641 tgtattactg cattattttt tgccatcggg tcatccttaa tggccccgcc aggggggcca
    14701 tgtggctgtt aaatgccatc aacgtacgaa atggtttctt tgtacactgg ctcaaccgca
    14761 cccagcttgc cgtagtaggt cgcaatctga tacagaccgc gatactggat aggaacgctc
    14821 tgcaacggca ccagcggata gcggacgtat tttttatcgt tggtgtacgc aaccatgcga
    14881 tccttattcc ccacaccacg gcctttcagc catttaaccg cgcggatatt cagcggaaca
    14941 ccgttctggt gatagctgat ggtgttggtc tgaaggtacg tcaacaggga ctggttaccc
    15001 gcagatgaaa cgatgatgct ggacaacaga gcaaactgct ctggcgggat cagcaaatca
    15061 cgcggaacca cagagtaacc ggaagcggcc cacgcatcag acagcacctg gttaatgctt
    15121 gcgcggattt cgtccggtgt tgagattgcc cacgttttgg cagcgttgtt gacaggcacg
    15181 ccgtccaggg taacaaggcc tttcaggttt aatgcggaat cgccaacata cacctgctcg
    15241 tcgttgtcca tctgccattt gagctgcatc ccgtcgtact tctgggtgtc aatcgggcga
    15301 cctacctgct gagctgctgc cagctctaca acggtccagc ccagttccat gccccagaga
    15361 ttcagcgggt taccggattt gccgatatcc acgtttacgc cagcaatagc ggttgagtct
    15421 ttgcctaccc agtttttgcc attcggattt gcaccagtac ccgcagcggc gaagctggta
    15481 ttcgtccagc tggaaatgtc atctgcgata gagacatctt cacgcaactg aatatcgcgg
    15541 gtccaggtgt accccgccag tggcaggttc agagtctggt cgagtcgctc cagctctccg
    15601 atgagaaagg caccagagct gtcaacggtt gcctgatcaa aagtaatcat tcgtctgttc
    15661 cttaaatctt ccaggaaatt tctgcattgc cgttagcatc accggcacct gtgaattcag
    15721 cgttggtcag caccacattt ttgccactga ctgacgtgga catgaatcca cccagcggca
    15781 ctttgatgga ttcatcagtg gagacgacaa cgtataccgg gtcgcctttt ttgatggtgc
    15841 tggcatcaaa atcagaaccg agattaacgg tcacgtagcc acgcttcatg gcgtcgcccg
    15901 ggaagttctt gcctgttccc acctggcgaa ccatgtccgg ctgcgaagtg gtcggataag
    15961 ggcgcacgta gatccccttc accttgtctg cggtatcgcc atctgccagc ggcacgaaaa
    16021 aaccgtcagc atcgtattta ccagccagcc cataggcagc aaaggcgtta tcggatttaa
    16081 ggaccaccgg ttcgacggtt aagtcctgcg ggcgagagat agccccggca atgccaacag
    16141 gcatccggta cagatatgca gtcattggat tatcctttgc ggttagacca gaattcagcg
    16201 ttttgtttgt tcagggaagc gatgctggtc atgcccatgt ttggacgttg tgcatcgccc
    16261 gtggtgctgc gggtgtttcg ccctttggca atctcagaca cagcgttaaa cgccatatcg
    16321 accgattgct taggcaattt gcggatatcc gcatcaccga caacctggcg aaccagcgtt
    16381 ttgtcagcag aagccagaac ctcgcgtttg aacacggtcg gtttcatctt acggctca
//
LOCUS       DAJTNV010000083        16375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNV010000083 DAJTNV010000000
VERSION     DAJTNV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO199_002929"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO199_002929"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5536897.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO199_002930"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO199_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5536898.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO199_002931"
     CDS             2337..2552
                     /locus_tag="OO199_002931"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536899.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO199_002932"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO199_002932"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5536900.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO199_002933"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO199_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5536901.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO199_002934"
     CDS             5918..6415
                     /locus_tag="OO199_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5536902.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO199_002935"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO199_002935"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5536903.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO199_002936"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO199_002936"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5536904.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO199_002937"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO199_002937"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5536905.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO199_002938"
     CDS             9100..9663
                     /locus_tag="OO199_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5536906.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO199_002939"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO199_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5536907.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO199_002940"
     CDS             complement(10255..11235)
                     /locus_tag="OO199_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5536908.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO199_002941"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO199_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5536909.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO199_002942"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO199_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5536910.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO199_002943"
     CDS             12538..12828
                     /locus_tag="OO199_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5536911.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO199_002944"
     CDS             12836..13429
                     /locus_tag="OO199_002944"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5536912.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO199_002945"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO199_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5536913.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="OO199_002946"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="OO199_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..>16375
                     /locus_tag="OO199_002947"
     CDS             15880..>16375
                     /locus_tag="OO199_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5536914.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQE"
BASE COUNT         4054 a         4218 c         4123 g         3980 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggagg
//
LOCUS       DAJTNV010000084        16197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNV010000084 DAJTNV010000000
VERSION     DAJTNV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..236
                     /locus_tag="OO199_002948"
     CDS             <1..236
                     /locus_tag="OO199_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5536915.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="OO199_002949"
     CDS             246..884
                     /locus_tag="OO199_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5536916.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="OO199_002950"
     CDS             887..2050
                     /locus_tag="OO199_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5536917.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="OO199_002951"
     CDS             2134..3759
                     /locus_tag="OO199_002951"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5536918.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OO199_002952"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OO199_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5536919.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OO199_002953"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OO199_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5536920.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="OO199_002954"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="OO199_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5536921.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OO199_002955"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OO199_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5536922.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OO199_002956"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OO199_002956"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5536923.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLTADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="OO199_002957"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="OO199_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5536924.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="OO199_002958"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="OO199_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5536925.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="OO199_002959"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="OO199_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5536926.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="OO199_002960"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="OO199_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5536927.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="OO199_002961"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="OO199_002961"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5536928.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="OO199_002962"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="OO199_002962"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5536929.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="OO199_002963"
     CDS             complement(12356..12631)
                     /locus_tag="OO199_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5536930.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="OO199_002964"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="OO199_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5536931.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="OO199_002965"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="OO199_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5536932.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="OO199_002966"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="OO199_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5536933.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="OO199_002967"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="OO199_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5536934.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..15031)
                     /locus_tag="OO199_002968"
                     /pseudo
     CDS             complement(14468..15031)
                     /locus_tag="OO199_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15095..>16197
                     /locus_tag="OO199_002969"
     CDS             join(15095..15332,15332..>16197)
                     /locus_tag="OO199_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5536935.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSP"
BASE COUNT         3819 a         3945 c         4364 g         4069 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag tcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgtta ctgaatcgcc acgggtttaa cagacacctc
    15061 agagtcattt aagatggctt aaagagaggt gcccatgagc ggtaagcgtt atcccgaaga
    15121 gtttaaaact gaagcagtca aacaggttgt tgatcgcggt tattctgttg ccagcgttgc
    15181 aacacgtctc gatatcacca cccacagcct ttatgcctgg ataaagaagt acggtccgga
    15241 ttcttccact aataaagaac agtcagatgc tcaggccgag atccgccgtc tccagaaaga
    15301 gctgaaacgg gttaccgacg aacgggacat attaaaaaaa gccgcggcgt acttcgcaaa
    15361 gctgtccgac tgaggtacgc ctttatccgt gacaactcct gttgctggcc tgttcgcctg
    15421 ctctgtcggg tgctggatgt tcatcccagt ggtttttacg cctggcttca gcagccgcat
    15481 tcacaacgcc atcaggcaga cctgagactg acaggacaga ttaaacagtt ctggctggaa
    15541 tcgggatgcg tctatggtta tcgcaaaatc catctggatc tgcgtgacag cgggcaacag
    15601 tgcggagtaa acagagtctg gagactgatg aaacgtgtcg gaataaaggc tcaggtcgga
    15661 taccgaagcc cgcgggcacg taaaggcgag gccagtatcg tgtcacccaa caggctccag
    15721 cgacagttca atccggatgc tcctgatgag cgttgggtaa cggacataac ctacatcagg
    15781 acccacgaag gctggctgta tcttgccgtt gttgttgatc tgttctcacg caaaattatc
    15841 ggctggtcca tgcaatcccg gatgacaaag gacattgtcc tgaacgcact gctgatggct
    15901 gtatggcggc gtaatcccga aaaacaggtg ctggttcatt cggatcaggg cagtcagtac
    15961 acaagccatg agtggcagtc gttcctgaaa tcacacggcc tggagggtag catgagccgt
    16021 cgcggtaact gccatgataa tgcggttgca gaaagttttt tccagttgtt gaaacgtgaa
    16081 cggataaaga aaaagatcta cggaacgcgg gaagaagccc gcagtgatat ttttgattac
    16141 atcgaaatgt tttataacag taagcgtcgg catggttcta gcgaacagat gtcaccg
//
LOCUS       DAJTNV010000085        16144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNV010000085 DAJTNV010000000
VERSION     DAJTNV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(114..380)
                     /locus_tag="OO199_002970"
     CDS             complement(114..380)
                     /locus_tag="OO199_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5536936.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="OO199_002971"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="OO199_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5536937.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="OO199_002972"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="OO199_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="OO199_002973"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="OO199_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5536938.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="OO199_002974"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="OO199_002974"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5536939.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO199_002975"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO199_002975"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5536940.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO199_002976"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO199_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5536941.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="OO199_002977"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="OO199_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO199_002978"
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO199_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5536942.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12642..14096)
                     /locus_tag="OO199_002979"
     CDS             complement(12642..14096)
                     /locus_tag="OO199_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5536943.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14093..14488)
                     /locus_tag="OO199_002980"
                     /pseudo
     CDS             complement(14093..14488)
                     /locus_tag="OO199_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            14601..14789
                     /locus_tag="OO199_002981"
     CDS             14601..14789
                     /locus_tag="OO199_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5536944.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            14799..15997
                     /locus_tag="OO199_002982"
     CDS             join(14799..15615,15615..15997)
                     /locus_tag="OO199_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5536945.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
BASE COUNT         4252 a         4079 c         3935 g         3878 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaactgct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaagatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatccggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 gcaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg
    13861 gatagagtaa aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata
    13921 acaataatct cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg
    13981 gacagaccac cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca
    14041 ataaataatc ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct
    14101 catcaacact gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt
    14161 cgtcaaatgc aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat
    14221 tttctatcgg aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac
    14281 cgtgaaacac ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca
    14341 ttcccgctat ttggttttct ttataatttg catcaaccca caactcactg actggtacta
    14401 gtgaaactaa cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga
    14461 caatgccatc aatcggcgca cgaaccacgg cagcctgacg gctgcaaatt gaaaaccgcc
    14521 cctgttcgtc ctgttgttgc cgcacgctcc gcagaatatg tatgctctga ggacctgttt
    14581 tattaaggac tcatcctctt atgttgaccc gcaaaagtat agataccgtt ctgctctctg
    14641 ttggtgctga gaagctctca caacgtgaat gggactggat gaagatgctc aaacccatgg
    14701 acccaccacc tgcgatggtc gcggcctcca tcctggagcg ccgtggcgac acagcagccc
    14761 ttacccggct acaggatacc gggggttaat ccgtcctcat gttgtccgct tttactcccc
    14821 gcccgctgaa acgtctgttc acggccaacc agtgctggac atccttcctg gatgcgggcg
    14881 gtctgcgcga tatcgaggtt gaggctgtca caaaaatgct ggcctgcggc acgcggatac
    14941 tgggcgtgaa ggaatacatc tgcgataaac ctgagtgccc ccacgtcaga tacgtcacta
    15001 actcatgcgg cagccgtgcc tgcccgtcct gcggaaaaaa ggccacagac ctgtggatag
    15061 cgacacagct gaatcgtctt cctgactgcg actgggtaca cctggtcttc accctgccgg
    15121 acacgcagtg gccggtgttc gaaagcaacc ggtggctgct gaatgacgtg tgccgtctgg
    15181 cggtggagaa tctgctgtat gccgcccgga aacgggggca ggaacccggt atcttctgcg
    15241 ccatccacac gtatggccgt cgtctcaact ggcatccgca tgtacatgtg tctgtaacct
    15301 gtggaggtct gaataagcat ggtcagtgga aaaagctgag cttcctgaaa gacgcgatgc
    15361 gttcgcggtg gatgtggaat atgcggcaac ggcttctgaa agcgtggtca gaggggctgg
    15421 cgatgccgga gtcgttgtca catatcacga cggaatcaca gaggagaagc ctggtgctga
    15481 aagccggagg aaaatactgg catgtgtaca tgtcgaaaaa aacggccggc gggaggaata
    15541 cggcgcgcta cctgggtcgt tatctgaaga agccgccgat agcggcctcc cggctggcac
    15601 attacaacgg agggggagcc tgagcttccg ttacctggac cacaaaacgg gagaaacggc
    15661 gacggaaacg ctgacacagc gtgagctggt cgcgaggctg aaacagcaca tcccggagaa
    15721 gttttttaag atggtgaggt acttcgggtt ccttgccaac cgggtgtgtg gagagaagct
    15781 gccgcaggtg taccgtgctc tggggatgga taaaccggaa ccagtggcga aagtgtgcta
    15841 tgcacaaatg gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg
    15901 ccggatggta taccgccggg ccatcgcggg actgaatgtg gaggggctga agaaaaacgc
    15961 gcgggatatc agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa
    16021 tcggtgcaag tataaaaaac agcctgttgc ggtataagaa tcgaccatgc agagagagaa
    16081 aacaccggag tggagggaaa agcagaaaag ctcacgtggc ataaggcgtg gacagcggta
    16141 tcgt
//
LOCUS       DAJTNV010000086        15632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNV010000086 DAJTNV010000000
VERSION     DAJTNV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1048
                     /locus_tag="OO199_002983"
     CDS             <1..1048
                     /locus_tag="OO199_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5536946.1"
                     /translation="VQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIYARQ
                     GVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGR
                     LWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETG
                     RVKEAGCLAHARRKIHDKDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKA
                     RSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGEN
                     ALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSN
                     QVHELLPWNFSSVK"
     gene            1103..1648
                     /locus_tag="OO199_002984"
     CDS             1103..1648
                     /locus_tag="OO199_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5536947.1"
                     /translation="METGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVEHLCPLCIA
                     DGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGWQQ
                     EFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDCQG
                     YLFRCLHCGKLRLWGDYFVVI"
     gene            complement(1702..2424)
                     /locus_tag="OO199_002985"
     CDS             complement(1702..2424)
                     /locus_tag="OO199_002985"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5536948.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(2426..3597)
                     /locus_tag="OO199_002986"
                     /pseudo
     CDS             complement(2426..3597)
                     /locus_tag="OO199_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(3624..5240)
                     /gene="bglH"
                     /locus_tag="OO199_002987"
     CDS             complement(3624..5240)
                     /gene="bglH"
                     /locus_tag="OO199_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5536949.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(5326..6720)
                     /gene="bglB"
                     /locus_tag="OO199_002988"
     CDS             complement(5326..6720)
                     /gene="bglB"
                     /locus_tag="OO199_002988"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5536950.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(6739..8616)
                     /gene="bglF"
                     /locus_tag="OO199_002989"
     CDS             complement(6739..8616)
                     /gene="bglF"
                     /locus_tag="OO199_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5536951.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8750..9571)
                     /gene="bglG"
                     /locus_tag="OO199_002990"
     CDS             complement(8750..9571)
                     /gene="bglG"
                     /locus_tag="OO199_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5536952.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(9858..10583)
                     /gene="phoU"
                     /locus_tag="OO199_002991"
     CDS             complement(9858..10583)
                     /gene="phoU"
                     /locus_tag="OO199_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5536953.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(10598..11371)
                     /gene="pstB"
                     /locus_tag="OO199_002992"
     CDS             complement(10598..11371)
                     /gene="pstB"
                     /locus_tag="OO199_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5536954.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(11463..12353)
                     /gene="pstA"
                     /locus_tag="OO199_002993"
     CDS             complement(11463..12353)
                     /gene="pstA"
                     /locus_tag="OO199_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5536955.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLVEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(12353..13312)
                     /gene="pstC"
                     /locus_tag="OO199_002994"
     CDS             complement(12353..13312)
                     /gene="pstC"
                     /locus_tag="OO199_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5536956.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(13399..14439)
                     /gene="pstS"
                     /locus_tag="OO199_002995"
     CDS             complement(13399..14439)
                     /gene="pstS"
                     /locus_tag="OO199_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5536957.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(14687..>15632)
                     /locus_tag="OO199_002996"
     CDS             complement(14687..>15632)
                     /locus_tag="OO199_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5536958.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         4046 a         3968 c         3630 g         3988 t
ORIGIN      
        1 cgttcaggca ccacttcctc ctaaaccgat cgaacgcggt tatgccagtg cagggttact
       61 tgcacggatc ctggtcagca aatatatgga acatatccct ttatatcgcc agtcagaaat
      121 atacgcgcga cagggcgtgg agctgagccg taataccatg gtgcgctggg tatcagaaat
      181 ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac tatgttctgg aggcaggaaa
      241 ggtgcacgca gatgacactc cggtgaaagt actggccccg gggaacggaa agacgaaaac
      301 gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg ggttcatccc tgccggcagc
      361 cgtctggttc gcgtattcgg cagatcgcaa aggagaacat ccgcagctcc acctggcaaa
      421 gtatcagggc gtactgcagg ctgatgccta tgcaggttat aacgtactgt acgaaacggg
      481 ccgggtgaag gaagccgggt gcctggccca cgcccgccga aaaatccatg acaaggatgt
      541 gcgccgtccg acagaaatga ctcaggaagc gctcagacgg atagcagagt tatacgacat
      601 agaagcggag atacgtggca gtccggcaga ggaacggctt gcagtcagaa aagccagaag
      661 cgtccagttg atgcagtcgt tgtacgactg gatacagttg cagaggaaaa cgctgtcgaa
      721 acatgcggag atggcgaagg cgttcgacta tatcctgaat cactggaatg cgctgaacga
      781 gttctgtcgt gacggctggg tggaaataga caacaacatc ggtgaaaacg cgttacgatc
      841 ggtggcggtt ggaagaaaaa attatctctt tttcggctca gacaagggag gagaaagtgc
      901 ggcgatcatc tacagtctgc tggtcacctg caaacagaac gaagtggagc cggaggactg
      961 gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg aaccaagtgc atgaactgct
     1021 gccctggaac ttctcgtctg taaaataatc cttacgctac gtacttttcg gggcgcttac
     1081 attcaaatat catcccaagc cactggaaac aggcgcattt gaacaggata aaaccgtaga
     1141 gtgcgattgc tgtgaacaac agacgtcagt ttattactcg ggtccctttt attgcgttga
     1201 tgaagttgaa catctctgtc cgttgtgtat tgcggacggt tctgctgcag aaaagtttgc
     1261 aggtagtttt caggatgatg ccagcataga aggtgttgaa tttgagtatg atgaagagga
     1321 cgaatttgcc ggtattaaga acacatatcc tgatgaaatg ctgaaagagt tggttgaacg
     1381 cacgccaggt tatcatggat ggcaacagga attctggctt gcgcattgtg gcgatttctg
     1441 tgcttttatc ggctatgtgg gctggaatga tataaaagat cgcctcgatg aatttgccaa
     1501 ccttgaagaa gattgtgaga atttcggtat tagaaattct gatctagcta aatgcctgca
     1561 aaagggtggt gattgtcagg gttatctctt ccgctgtctc cactgcggca agctgagact
     1621 gtggggtgat tatttcgtag ttatttaaat aatgagaaca ggccagagcg taattcatac
     1681 atccggcctt atttcttcag cttaaccgaa cgctaattct gcggcagcgg ctttatcagc
     1741 gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt cggtaaccgg
     1801 accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa ttatcagcaa
     1861 gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag gcactaacga
     1921 aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct ggatcgggaa
     1981 ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa aatgaccatc
     2041 tgcacctaat cccagcacca ccaaatccag gccgccttca cgcgccagtt tctgatcatg
     2101 ctcgcggtag ttatcaatag tgagcttctg gatattctct tctttgatcc ccgcaggggt
     2161 gaaaaacaga ttacgcagat tggtaatcgt tacgccttct ccctctttgc cgcgaaaggg
     2221 aatttcatcg aaattataga aatagcagtt atcgtaccaa ggcttacctt taaccagggt
     2281 ggtgaggtat tcatacatgc ctttgggcgt gctaccggcg gtaattgcca ggttaacacg
     2341 acgcgtcttc gacatataac caagcagatg gtgtgccgcg acacggctca tttcctggta
     2401 atcttcggta atgattaatt tcatcttaat gccttatttt aaatttctgt acgaaggctg
     2461 cataggcagg ccgccagaca tccatttcat ggttcaggcc gggatattcc tggtaatcaa
     2521 acttaatttt tttctgctca agctcagttt tcagcccggc gatatccttg ccggttacga
     2581 catctttatc accaacgacc acagtaaaat tacgtagttg ctggttaata gctgccggat
     2641 cgttcagccg ggccgcgaca ccttcatccg gtacggttgt cgtggtaaca ccactgaatg
     2701 tggccagcca aagctttcca gatgattcat tccggaaacc agcgcctggt acccgccttg
     2761 tgaaagccct gccagcgcgc gaccatcggc atctttacgg acactaaaac gcttgctaat
     2821 cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattca gcggataaaa
     2881 gactttacgt ctttcctgag gcacgaaatc ttcgggaata atgcccttcg catcggtttc
     2941 ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca ggttatccat
     3001 gatttgcggg atacgcccct gatcgatagc ggaacgtccg gtatcaccaa aaccgtgata
     3061 gaaatagagc actggcaaag gctcgcccat gccggtgtat cctggcgggg tccagacata
     3121 catctgacgt tcagattgca atgcgttgga gtggtaagtt atggcgatca aatcaccatg
     3181 cgcaacagaa cgcgtatcca gataactgcc tggcaccaga atcatactgg agttaacctg
     3241 gcgctgaggc ttagtcattg cggtgcctgt atcaatgctg cgtacaccat caacattgaa
     3301 aaaatactcg tacaggttgc ctttcaggat aggtgtgcgc cacgaccaga ctcctgcttc
     3361 gtctttggtc atcgggtgaa tattgtccgg aaccggaaca cccaccacta cagagacatt
     3421 ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggcattga cctgagtcac
     3481 atactgtttc accgggatag tgggtgccgg gctggctggc atatccgctg ctatggccca
     3541 ctgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta tattcatcgt
     3601 aatattcctt tcattaagtt ccattaccac cagatttcag cctgggcacc gacagcaaac
     3661 tgggcgtctt tattgtcttc gaaggtgaag ccatccagtt cgttttccag ggctttgata
     3721 tacgtggcgt agaaacgaat ttccagacgc gaggtcaaca tactggtatt gactttaaag
     3781 gtatggaaga gcgtggtttt ataaccagac tcattaaatt tattactatt cgcatctttg
     3841 ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc ccaaatatag
     3901 gccgggcgaa caaccgcacg aatagattcg aaatcagagt gtgcgcctgt ttcgtagcta
     3961 taaatattgt taccgaagga gtaaacaatc gcgttagcca caataaaatg atcgccgata
     4021 taggcttcgc cctgcgaggt cagacgaact gctgttccgc cggtgtgatc accataataa
     4081 cgaccattaa aggtggtaaa tggacttgcg ccagcataac ggccaaagtt cctggcgata
     4141 gagttattcg caaccaggaa ggagaattcg ttgaagccac ctttatcaaa tttctgcgtt
     4201 aaagatgtgc cgaacatcca ggtatctttc cagtcataat acccgttatt atcctgatta
     4261 tctttttccg atgcgctttc gtttgccgtg acataacgac cactcaccat taaggtcgct
     4321 ttatcccata acgggatatc tttatagcgt aaatcaatag tatgggtatt aatctgctgt
     4381 ttgttttgca ggctgcgatc gtaatcatcg acatcttcgc gaaccagcgc gatatcaatt
     4441 ttacccggac cgactttcca gttttccaga cctacacccg ctgcagcatc agtacgctgc
     4501 gttttccagt ccagcatctg gatttcaatt ttcggcgcac cgtgtttacc cacccagaaa
     4561 tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc ggagaactgc
     4621 ataaagttct cgccaccggc attatcgcca aaccagccgg tagagtactg ctgaccaacg
     4681 ttaccatcta tcatcacaac ggcatcaacc cgtttgccgt tttcgttgta gacacgttgt
     4741 tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag agaaccaatc
     4801 gcccatgatt taggtgaacc atgagaggcg gtcccccaac cagaacggta atagccgttg
     4861 taactaaagc caatttcatc tttcacaaac ttactgaaat ctttcagagt catggaagaa
     4921 taaatcgagc ctgtctcact ggcatttttc tcttcatttt tgaccagtac agcatcaggt
     4981 tttgcggcac tactggtcgg gaacggattg gcggcatacc cttgatcatt cgtacttacg
     5041 ctacgattca ccgtcgctgg cgtatatttt ttcttctctt catctttata ctttttgagt
     5101 tcgctttgcg tttctcttaa cgctttttct aataactcaa ggcgttgttc caccgttaat
     5161 gattcagcca atgattgtgc agaaaaggct aacggtgcca ttaataagat ggcagaggta
     5221 ataagatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt aacgtatcgc
     5281 atccagactg ttctgaatgc gacgataatt aaggtgcttt attggttatt tttttaatga
     5341 cagcccccgc gttttgatca cttctgcata ccatccgaag cttttcttac gcgttcttgt
     5401 gaggcttcct tcgccattat catcacgatc cacataaata aagccgtagc gcttagacat
     5461 ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc ccataatatc
     5521 cacaccatcg gcaatcgctt catttacctg taccaggtga tcgtttaaat aggcaattcg
     5581 ataatcgtcc tgtatcgaac catccgcttc aacgctgtct tttacgccta atccattctc
     5641 gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa cccgtaatcc
     5701 aaccggatca atttgccacc cccactctga acttttcaga tgcggattgg ggatcatatt
     5761 cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac aaccagtcat
     5821 gtaataacta aaagagatga aatctacagt atgttttaaa tcttctgcgt cactttcagt
     5881 catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg gatattggcc
     5941 acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg cctgcaacat
     6001 atcctgtggc tggcaggtga gggggtaaac cagcccaccg agaagcatat tgccgatttt
     6061 cgcttcgggg atcaggctat gataggcttt aactgcccgc gcactggcaa ccagttgatg
     6121 atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca cgcccgtgaa
     6181 tggcgcgtgt aacgacatat tgatttcatt aaacgtcagc cataacgcta ctttatgttg
     6241 gtagcgggta aagaccgtgc gggcgtaatg ctcgaagtga tcgatgaccg cccgattagc
     6301 ccaaccgccg tagtttttca ccagcccata tagcatttcg taatgggata acgttaccag
     6361 cggcttgatc cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg ctaaccccgc
     6421 ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa tggaaatacg
     6481 cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac ggtaataaaa
     6541 atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca tttttcccat
     6601 tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcca gccaggcacc
     6661 ttccacctga ttggcagctg ttgcaccacc ccaaagaaat gtttctggaa atgctttcat
     6721 aattaactcc ttttatcgtt agcgaatgat ggataacagc ggttcacctg cgcttatctg
     6781 cgccgtgccg tggggtaata cgtctgtaaa atcatcgcta ttactgatta ataccggcgt
     6841 cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca gccgatcgcc
     6901 tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt ccagttttac
     6961 ggtgtcgata ccgacatgaa tcaggatctc cacaccatca tctgactcaa tgccaatggc
     7021 gtgtaatgtg gcgaacaacg aagcaattcg acccgcaacc ggagaacgca cttcaccaac
     7081 cgagggcaga atggcgatac ctttacccaa caggccactg gcaaacgtgg tatcagcgac
     7141 gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag ataccgccct gctcaggtgg
     7201 tgtaataacc tctggtgttt tctcttgcgg ggcaccctgc gctggctgac gtttactggt
     7261 gatgaaatga agcatcaacg taccaacaaa tgcgcaacct atggcaatga caccgccaat
     7321 aacgctggcc cagacggtga aatcaatccc cgttgacggg atggtctgca tgaaggtgaa
     7381 aatacttggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga tggtggcccc
     7441 caatgctcca ctgatacagg cgataacaaa ggggtactta cgcggcaggt tgacgccata
     7501 taccgctggt tcggtgatac caaacagacc cgtcaacgcc gctgatcccg ccaccacttt
     7561 tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc gccccgacct gcgccataat
     7621 ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt tattgataca
     7681 cagcggcacc aggcccctgt gcagtccgaa catgacgaag atttgccaga agccgcccat
     7741 taccgcgccc gcaaatgcag gaaccgcctg ataaagccag agataaccgg cggcaatcat
     7801 ttcgcttatc caggttgata gcggccccac cagcagaaag gtgacgggtg tgataaccat
     7861 cagacatagc aatggtgtga agaaattttt gattgccgac ggtaaccacg cattaagtcg
     7921 gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg atgacgagta
     7981 attcaacaat gtgaccggaa tacccaggaa atccagcccc ggcgcatccg ctttttgccc
     8041 gttctcgaag gcagtcagaa ttaatggatg cactaacgct ccaccaatca ccatggcagt
     8101 aaatggatta ccgccgaagc gtttgcccgc ggtgtatccc aggattatcg ggaagaaccc
     8161 aaacaaggca tcactggcgc tgaataaaat taaataagta ccactttgtt cggtcgtcca
     8221 ctgaaaagtg agcgccagag ccagcatacc tttcaggatc ccggttgccg ccatcagacc
     8281 gatcagaggc gtaaaaatac ctgaaataac ataaacaaag cggtttagca gattaccttt
     8341 ctcatcattt tccggtgcct gttgcgcttt ttcgccaagg cctgccacgc tgttaaccgc
     8401 caggaagaca tcggccacat ggttacctat gaccacctga aactgaccac cgctttccac
     8461 caccataata ataccggggg tctttttcag tacctcagct tgcgctttgc tttcatcctt
     8521 taacttaaaa cgtaatcgcg ttgcgcaatg catcagactc acaatgttat ctgcgccccc
     8581 gactcctgcg actatttttc tggctaactc cgtcataact tgccctctac cgctttgcgg
     8641 caaaactcca aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca atttttttca
     8701 ggttttgccc gcttagtgcg gtaacaatcc tttactcagt aataatattt cagtgttctt
     8761 tgcgcacgcg ctctatattt atggctaaaa acataatctc tgcgggtgaa attttacgtt
     8821 gatactgcaa accaataaaa atggcgatcc gttccgcaca ttgccatgct tgcgggtaat
     8881 tttgttttac tgcttgttgt aatgattcat cactatcgtt aattgaagca tgttcaagaa
     8941 tacgccaaga taaaaacttc agatgtgtaa ccagtcgctg ataactcaag ctttcttcct
     9001 ggtaattaag gctgaactga aattttatta attgcagcat ttcgcgcatt aactgcgtga
     9061 cacctgcaac atcctccata tttccgctca tttgggcact gaccagatgc atggcaataa
     9121 agcccacttc atctttcggt aactgcacgc ccaaccgttt atcaataatg gttaacgctt
     9181 cttcccctag ctggaactct ttcgggtaaa gccgctggat atcccacagc aacacgtttt
     9241 gctgaaagcg tttaatcgca aactggcaat ggtcagttag cgagatataa atactgtcct
     9301 gtaattttcc caagcgctcc tgcgctaaag agataagacg atcacaggtt gccatcacct
     9361 caagaggaat atgacttaag agttcgctta atcgcccgtt cagttcatga ctgctcaagg
     9421 catactcttt ttctattcca cttgagttaa ttctttcgcc agcgcgtttt tgaaagccaa
     9481 ttccgcgccc catgacgact ttttcccgct gttgatcatc aataaccacc acaacattat
     9541 tgttgagaat tttggtgatt tgcatgttca tagcaaggac cttttttata aacaaaaaaa
     9601 aaccgacttc accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat
     9661 ccagtcattt attaatggtt tttataacga acatccaggt tcggaaatta atttaattgc
     9721 gtgcagagaa aatagcaatg cgctattgat aaaaatatga ccatgctcgc agttattaac
     9781 tttatgtaat tttaggaatt tataaagtta tatataacaa atcccaataa ttaagttatt
     9841 gggatttgtc tggtgaatta tttgtcgcta tcttttcccg ccagcagttt atccagctca
     9901 tcaccgccta catgacggaa atcctgaccc ttcacgtagt agaagataaa ctcacaaata
     9961 ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac agaacagcgc ggtaagtacg
    10021 ctcggaatag tgcgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
    10081 tattcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
    10141 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
    10201 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
    10261 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc
    10321 agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
    10381 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg
    10441 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
    10501 atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt
    10561 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
    10621 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
    10681 tcaattcgcc caggtacata aatgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
    10741 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
    10801 gcccggtaga gatggggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc
    10861 gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag agagagtaac
    10921 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
    10981 gcacgcgctc gtccatgtcg gcacaggaga gcttctcaaa cagacgaacg ccaaaggcga
    11041 tgttgtcgta gatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
    11101 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt
    11161 caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
    11221 tcgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg gcgatatcca
    11281 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
    11341 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tacctgatgc
    11401 ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt tcacgccgca tccggcaaag
    11461 tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca gaatgttcag cagcaggacg
    11521 cacagggtaa tgatcaatac cccggcccag gccagttgtt gccattccgc aaacgggctc
    11581 atcgcaaact taaagatcgt caccggcagg ttggcgatcg gctgcatcat gtccgtgctc
    11641 cagaactggt tggagagcgc ggtaaacagc agcggcgcgg tttcaccggc aatacgggca
    11701 atcgccagca ggataccggt cataatcccg gacaccgacg ctttcagcgt aatcgcggag
    11761 atcatcttcc acttcggtgt acccagcgca taagccgctt cacgcaggct gtccggcacc
    11821 agtttcagca tgttctcagt ggtgcggata acgatcggca cctgcaacag cgccagcgca
    11881 attacgcccg cccagccgga gaagtgctcc atctgtgcca ccacgatggt gtagacaaac
    11941 agaccaacca caatcgacgg cgcggagagc agaatgtcgt taatgaagcg aatcacttct
    12001 gccagccagg atttacgacc atattccacc agataaatcc ccgccataat gcccagcggc
    12061 gtaccgaata ccgtggccca caaaattaac agcccgctac ccgccagggc gttcgccaga
    12121 ccaccacctt ccgtattggg cggcggcgtc atttcagtga acagcgccag cgacataccg
    12181 tcgataccgc gagtgatggt ggacattaaa atccagatca gccagaacag gccgaaggcc
    12241 atcgtcgcca tcgagagcgt cagcgcaata cggtttttga ggcggcgacg cgcctgcatt
    12301 ttgcggcgag attcagccag cgccgcagtg gtttgcattt caaccatagc cattagcgtg
    12361 ccccctcatt cttagccagg cgcataatca taaacttcga tgcggcgagg acgatgaagg
    12421 taatcacaaa caggatcagg cccagttcca tcagtgcggc aacgtgcaga ccggattccg
    12481 cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt gcccggcatg tacagcgagg
    12541 cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac cgccatggtt tcaccgagcg
    12601 cgcggcccag ccccagcatg atgccgccga taacaccatt tttggtgaac ggaagaacga
    12661 tacgccagat aacttcccag gtggtgcagc caataccgta ggccgactct ttcatcatca
    12721 ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat gtacggaata atcatgatgg
    12781 cgaggatcac gcctgccgcg aggataccga taccaaatgc ggggccagag aacagcgcgc
    12841 caacaatcgg gatattcgac atgatattgc cgaccggctc ctgaaagtaa acggcgaaca
    12901 gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat acttggaatg gctgccagca
    12961 gctcaatggc gatacccagc gggcgtttca gccagccagg cgcaagctca gtcaggaaca
    13021 gggcgatacc gaaactcacc gggacggcga tcagcagcgc gataaacgaa gtcaccaacg
    13081 taccgtagat cggcaccagc gccccgtaga tatcgttcgg tgcatcccac tctttggtcc
    13141 ataggaaagc cagaccaaat ttctgaatgc tcggccagga ggagatgatc agagagacaa
    13201 taatgccacc caacatcaat agcacaatca gcgccgccag ttttaccagc acgctgaaaa
    13261 ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt ggttgcagcc ataagttact
    13321 cttcagttaa acgcgtttac aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt
    13381 acccggcctg gagttttatt agtacagcgg cttaccgcta ctgtctttaa tattggtctt
    13441 ccacgcagcg cgaacctgtt caactacact atccggcagg ctggcgtaat ccaggtcgtt
    13501 cgcctgttta gccccggttt tgtacgccca gtcgaagaat ttcagcacct ctgtgccttg
    13561 ttctggtttc ttctgatctt tgtggatcag aatgaacgtg gtagaggtaa taggccatgc
    13621 atcttcgcct ttctggttgg tcagatcctg agcgaaggtt ttgctccagt ctgcaccttt
    13681 tgctgcatta gcgaagtttt cttcggtcgg actaaccggt ttaccatcag cggagatcag
    13741 tttggtgtac gccaggttgt tctgcttcgc gtaagcatat tcaacataac caattgcacc
    13801 cggcagacgc tgaacgaacg cggcgatacc gtcgttacct ttaccgccca gaccgatcgg
    13861 ccattttacg gtagagccag taccaacgtt gtttttccac tcttcgttca ctttcgccag
    13921 gtagctggtg aagacgaagg aagtcccgga gccatctgcg cggcgtacta cagcaatgtt
    13981 ttgtgaaggc agtttcagac ccggattcag tttggcgatg gcttcatcat cccacttctt
    14041 gattttgccc aggtagatgt cgccgagggt tttaccatcc agcaccagtt cgccagactt
    14101 cagccctgga atgttaaccg ccagcaccac gccgccaatc acggtcggga actggaacag
    14161 accttcctga gccagttttt cgtcagacag cggcgcgtca gaggcaccaa aatcaacggt
    14221 attagcgata atctgtttta cgccaccgga agaaccgata ccctggtagt taactttatt
    14281 accggtttct ttctggtaag tgtcagccca tttggcatac accggcgcag ggaaggttgc
    14341 acctgcacct gtcaggcttg cttctgcaaa cacagagaaa gcactcatcg ataaggtcgc
    14401 ggcgacaaca gttgcgacgg tggtacgcat aactttcata atgtctcctg ggaggattca
    14461 taaagcattg tttgttggct acgagaagca aaataggaca aacaggtgac agttatatat
    14521 aaggaatatg acatttttat gacagaaagg aattaccaaa taacttactg attttaaatg
    14581 tgaagatgaa aatcccacat gccatctttt tgtcatgatg catttaaaag tgataaagaa
    14641 atgggtgaga tgacaacgat gtgatgaaca atcgttgtca gacctattag cgcacaatct
    14701 caacagttat cgtcgctgtt gcagtaaatt gccccggggc tgggcgagct ccagtaaggc
    14761 tcgcaggagc agctgaaaaa gttacactac ctgatttttc accatacact gttgatttat
    14821 ccatgtctgt tggcaactca ccaccgttca ctttgatttc cttattattt ttgtcgtaaa
    14881 tttttatccc cacgttagag tttgaagtta cgaccattga attgtccgta ttgggttcgg
    14941 tagcataaaa agacgtactg atatcatcgg tattcagtat ccccaggttt tcacactcaa
    15001 tagtgactgt tttagtaatt ttatgtgttg ttaccgcact tccaacaaca gatgaaaaat
    15061 ctgcagctgg tatttcttta aaatcaaaac aaactggctt taaattattt atttcgcaac
    15121 tttgcggagc tgtaatatcg ccccgaatat aaacatcagc catcggctgt gagtaatcca
    15181 ccggggtgtc acggctaatt gttccataca atgtcactat ctttgtttca ggaataatca
    15241 attttcctgc aaatgtcttc ttgatataaa aatatactat cgctttagcc ccactggata
    15301 tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt accagacggg ttgttagcat
    15361 gatattcaaa aggtgcattc acatacccaa ccccaagaat atctatagca atcccaacat
    15421 caaggttcgg attaagatca taataattaa tattagagtg aaccccagca taagttaatg
    15481 cctggtttat ttttgacgta taatagatat aggggaattc tcctacgtta ccagtatcac
    15541 aatgacagcg tccaagatat gaggatccat tagttacagt ggtgttaaat gtcgttccaa
    15601 ccttattttc gttcgcggta ataagatatc cc
//
LOCUS       DAJTNV010000087        15523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNV010000087 DAJTNV010000000
VERSION     DAJTNV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO199_002997"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO199_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5536959.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO199_002998"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO199_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5536960.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO199_002999"
     CDS             complement(941..1408)
                     /locus_tag="OO199_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5536961.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO199_003000"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO199_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5536962.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="OO199_003001"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="OO199_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5536963.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="OO199_003002"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="OO199_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5536964.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="OO199_003003"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="OO199_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5536965.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="OO199_003004"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="OO199_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5536966.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="OO199_003005"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="OO199_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5536967.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="OO199_003006"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="OO199_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5536968.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="OO199_003007"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="OO199_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5536969.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="OO199_003008"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="OO199_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5536970.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="OO199_003009"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="OO199_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5536971.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO199_003010"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO199_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5536972.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="OO199_003011"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="OO199_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5536973.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="OO199_003012"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="OO199_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5536974.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="OO199_003013"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="OO199_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5536975.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="OO199_003014"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="OO199_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5536976.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="OO199_003015"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="OO199_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5536977.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="OO199_003016"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="OO199_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5536978.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="OO199_003017"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="OO199_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5536979.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="OO199_003018"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="OO199_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5536980.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="OO199_003019"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="OO199_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5536981.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="OO199_003020"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="OO199_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5536982.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="OO199_003021"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="OO199_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5536983.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO199_003022"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO199_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5536984.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="OO199_003023"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="OO199_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5536985.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="OO199_003024"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="OO199_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5536986.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="OO199_003025"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="OO199_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5536987.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="OO199_003026"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="OO199_003026"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5536988.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15060..15443
                     /gene="rplQ"
                     /locus_tag="OO199_003027"
     CDS             15060..15443
                     /gene="rplQ"
                     /locus_tag="OO199_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5536989.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3644 c         4130 g         3741 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt actattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccaatcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcga
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15061 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15121 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15181 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg
    15241 ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta
    15301 acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg
    15361 gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga
    15421 aagcagaagc tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt
    15481 ttttataccc gtagtatccc cacttatcta caatagctgt act
//
LOCUS       DAJTNV010000088        15508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNV010000088 DAJTNV010000000
VERSION     DAJTNV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..73)
                     /locus_tag="OO199_003028"
     CDS             complement(<1..73)
                     /locus_tag="OO199_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5536990.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO199_003029"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO199_003029"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5536991.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO199_003030"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO199_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5536992.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO199_003031"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO199_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5536993.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO199_003032"
     CDS             2703..2939
                     /locus_tag="OO199_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5536994.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO199_003033"
     CDS             2942..3799
                     /locus_tag="OO199_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5536995.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5796
                     /locus_tag="OO199_003034"
                     /pseudo
     CDS             3799..5796
                     /locus_tag="OO199_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="OO199_003035"
     CDS             complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="OO199_003035"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5536996.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6399..7295)
                     /locus_tag="OO199_003036"
     CDS             complement(6399..7295)
                     /locus_tag="OO199_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5536997.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7344..7583)
                     /locus_tag="OO199_003037"
     CDS             complement(7344..7583)
                     /locus_tag="OO199_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5536998.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7686..8285
                     /gene="nemR"
                     /locus_tag="OO199_003038"
     CDS             7686..8285
                     /gene="nemR"
                     /locus_tag="OO199_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5536999.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8322..9419
                     /gene="nemA"
                     /locus_tag="OO199_003039"
     CDS             8322..9419
                     /gene="nemA"
                     /locus_tag="OO199_003039"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5537000.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9500..9907
                     /gene="gloA"
                     /locus_tag="OO199_003040"
     CDS             9500..9907
                     /gene="gloA"
                     /locus_tag="OO199_003040"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5537001.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10010..10657
                     /gene="rnt"
                     /locus_tag="OO199_003041"
     CDS             10010..10657
                     /gene="rnt"
                     /locus_tag="OO199_003041"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5537002.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10750..15208
                     /locus_tag="OO199_003042"
                     /pseudo
     CDS             10750..15208
                     /locus_tag="OO199_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15252..>15508)
                     /locus_tag="OO199_003043"
     CDS             complement(15252..>15508)
                     /locus_tag="OO199_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5537003.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3554 a         4108 c         4078 g         3768 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa
     5101 tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc
     5161 aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact
     5221 aatcccccgg tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc
     5281 gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc
     5341 cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca
     5401 ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc
     5461 caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct
     5521 catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg
     5581 gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca
     5641 ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa
     5701 cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt
     5761 gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata
     5821 gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg
     5881 gaccatcagc gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc
     5941 atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt
     6001 accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc
     6061 cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg
     6121 gaagccatgt tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc
     6181 ggtttcagta atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt
     6241 catctcgact ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc
     6301 cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca
     6361 gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt
     6421 gcagctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca
     6481 gctgcccgta cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa
     6541 cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc
     6601 acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca
     6661 aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc
     6721 agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc
     6781 agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc
     6841 actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac
     6901 gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac
     6961 tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct
     7021 tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga
     7081 ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca
     7141 gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg
     7201 cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc
     7261 gggccttccg gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa
     7321 gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac
     7381 gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct
     7441 tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag
     7501 ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga
     7561 aagaactcta attgctccgc cacctcgccc tcctcagata agattattac cattattgaa
     7621 gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct actacactcc
     7681 aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc
     7741 aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg
     7801 aagtgccgaa agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca
     7861 tgcttgagcg tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg
     7921 aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc
     7981 aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt
     8041 cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc
     8101 aggctctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac
     8161 aggcacaagt gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca
     8221 gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg
     8281 tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt
     8341 attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga
     8401 cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     8461 gccaacgtgc cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa
     8521 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     8581 tcaccgctgg cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac
     8641 gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta
     8701 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat
     8761 ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg
     8821 ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt
     8881 atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca
     8941 gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg
     9001 gtgccgatcg cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg
     9061 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     9121 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     9181 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     9241 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     9301 ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc
     9361 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     9421 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     9481 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     9541 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     9601 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     9661 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     9721 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     9781 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     9841 agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg
     9901 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     9961 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
    10021 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
    10081 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
    10141 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
    10201 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
    10261 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
    10321 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
    10381 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
    10441 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
    10501 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
    10561 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
    10621 gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg
    10681 caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga
    10741 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
    10801 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
    10861 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
    10921 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
    10981 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
    11041 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
    11101 cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt
    11161 gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga
    11221 attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat
    11281 cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta
    11341 ccggtcgcca atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat
    11401 gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg
    11461 cgccatcgct cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca
    11521 cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg
    11581 gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt
    11641 attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg
    11701 ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat
    11761 atgggcgcgg tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta
    11821 caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc
    11881 cgtacccggc gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg
    11941 ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc
    12001 gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca
    12061 ccgtggaaag atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc
    12121 tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg
    12181 atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg
    12241 gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg
    12301 gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc
    12361 ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct
    12421 ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa
    12481 ttaggcgaga tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg
    12541 actattcccc cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac
    12601 gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc
    12661 cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat
    12721 gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct
    12781 gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc
    12841 gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta
    12901 ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg
    12961 ccgggccgca cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag
    13021 ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa
    13081 tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt
    13141 ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta
    13201 ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg
    13261 gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg
    13321 ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct
    13381 cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca
    13441 atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat
    13501 ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt
    13561 cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag
    13621 tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc
    13681 tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt
    13741 attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg
    13801 caacaaaaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa
    13861 gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa
    13921 cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcca
    13981 ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt
    14041 ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca
    14101 gaaatgctcg atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct
    14161 gggtgaagat gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac
    14221 tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct
    14281 ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt
    14341 cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt
    14401 cattaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg
    14461 cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt
    14521 ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt
    14581 ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    14641 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    14701 gcaaacactt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    14761 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    14821 gacacaagcc acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc
    14881 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg
    14941 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    15001 cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca
    15061 ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa
    15121 tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    15181 ttcgccacaa gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt
    15241 ccctgctacg ttcaagctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
    15301 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    15361 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    15421 gacaggcggt tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct
    15481 ggcggaacag caagcacctg accgccac
//
LOCUS       DAJTNV010000089        15381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNV010000089 DAJTNV010000000
VERSION     DAJTNV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..1175)
                     /locus_tag="OO199_003044"
     CDS             complement(<1..1175)
                     /locus_tag="OO199_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5537004.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO199_003045"
     tRNA            complement(1374..1458)
                     /locus_tag="OO199_003045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO199_003046"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO199_003046"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5537005.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO199_003047"
     CDS             2802..4304
                     /locus_tag="OO199_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5537006.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO199_003048"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO199_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5537007.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO199_003049"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO199_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5537008.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO199_003050"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO199_003050"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5537009.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO199_003051"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO199_003051"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5537010.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO199_003052"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO199_003052"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5537011.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO199_003053"
     CDS             complement(11869..13065)
                     /locus_tag="OO199_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5537012.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO199_003054"
     CDS             13258..13761
                     /locus_tag="OO199_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5537013.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO199_003055"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO199_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5537014.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..>15381
                     /gene="argF"
                     /locus_tag="OO199_003056"
     CDS             14385..>15381
                     /gene="argF"
                     /locus_tag="OO199_003056"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5537015.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATL"
BASE COUNT         3961 a         3930 c         3965 g         3525 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc a
//
LOCUS       DAJTNV010000090        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNV010000090 DAJTNV010000000
VERSION     DAJTNV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO199_003057"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO199_003057"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5537016.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO199_003058"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO199_003058"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5537017.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO199_003059"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO199_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5537018.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO199_003060"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO199_003060"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5537019.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO199_003061"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO199_003061"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5537020.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO199_003062"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO199_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5537021.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO199_003063"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO199_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5537022.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO199_003064"
     CDS             complement(8065..9207)
                     /locus_tag="OO199_003064"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5537023.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO199_003065"
     CDS             complement(9216..10229)
                     /locus_tag="OO199_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5537024.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO199_003066"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO199_003066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5537025.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO199_003067"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO199_003067"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5537026.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO199_003068"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO199_003068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5537027.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO199_003069"
     CDS             complement(12827..13951)
                     /locus_tag="OO199_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5537028.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO199_003070"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO199_003070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5537029.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4378 a         3446 c         3146 g         4374 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg gaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgctaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcagc aagaattggc gggtaaattg ctatttaagt caaa
//
LOCUS       DAJTNV010000091        15124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNV010000091 DAJTNV010000000
VERSION     DAJTNV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(172..1068)
                     /gene="yhaJ"
                     /locus_tag="OO199_003071"
     CDS             complement(172..1068)
                     /gene="yhaJ"
                     /locus_tag="OO199_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5537030.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1173..1874
                     /locus_tag="OO199_003072"
     CDS             1173..1874
                     /locus_tag="OO199_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5537031.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1897..2061
                     /locus_tag="OO199_003073"
     CDS             1897..2061
                     /locus_tag="OO199_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537032.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2273..3583)
                     /locus_tag="OO199_003074"
     CDS             complement(2273..3583)
                     /locus_tag="OO199_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5537033.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3611..4942)
                     /locus_tag="OO199_003075"
     CDS             complement(3611..4942)
                     /locus_tag="OO199_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5537034.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5217..6581)
                     /gene="tdcG"
                     /locus_tag="OO199_003076"
     CDS             complement(5217..6581)
                     /gene="tdcG"
                     /locus_tag="OO199_003076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5537035.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6653..7042)
                     /locus_tag="OO199_003077"
     CDS             complement(6653..7042)
                     /locus_tag="OO199_003077"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5537036.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7056..9350)
                     /gene="tdcE"
                     /locus_tag="OO199_003078"
     CDS             complement(7056..9350)
                     /gene="tdcE"
                     /locus_tag="OO199_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5537037.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9384..10592)
                     /gene="tdcD"
                     /locus_tag="OO199_003079"
     CDS             complement(9384..10592)
                     /gene="tdcD"
                     /locus_tag="OO199_003079"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5537038.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10618..11949)
                     /gene="tdcC"
                     /locus_tag="OO199_003080"
     CDS             complement(10618..11949)
                     /gene="tdcC"
                     /locus_tag="OO199_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5537039.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11971..12960)
                     /gene="tdcB"
                     /locus_tag="OO199_003081"
     CDS             complement(11971..12960)
                     /gene="tdcB"
                     /locus_tag="OO199_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5537040.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13059..13997)
                     /gene="tdcA"
                     /locus_tag="OO199_003082"
     CDS             complement(13059..13997)
                     /gene="tdcA"
                     /locus_tag="OO199_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5537041.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14186..14530
                     /gene="tdcR"
                     /locus_tag="OO199_003083"
     CDS             14186..14530
                     /gene="tdcR"
                     /locus_tag="OO199_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5537042.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14786..>15124
                     /locus_tag="OO199_003084"
     CDS             14786..>15124
                     /locus_tag="OO199_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537043.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPF"
BASE COUNT         4018 a         3988 c         3473 g         3645 t
ORIGIN      
        1 tgacaaagtg cgctttgttt atgccggatg cggcgtaaac gccttatcca gcctacaaat
       61 tgccaaaatt caatatattg cagtgactgc gtaggcctga taagcgtagc gcatcaggca
      121 gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg tgcttaatag attattttcc
      181 agcgaacaac tttggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg
      241 acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg
      301 cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc
      361 cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag
      421 cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata
      481 tttcacacgc gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc
      541 ggcaacgtag acgttcatta acgtatagag cttgcgcgag ttgatctccg acgaggaacg
      601 aaaatgcata tccggcgcga taacgatatc cgcccgcccc tgctccagcc gttcccacgc
      661 ccccgccagc acttctgtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc
      721 gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt
      781 ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt ttatctgcgg cttccagcaa
      841 aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga
      901 gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc
      961 ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa ctgccccggc gatcgatcgc
     1021 atccataacc cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt
     1081 caggaaattt gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta
     1141 ccataaaagg aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt
     1201 gtggacaagc agactacgga tggttgcagg cccggtatac tttttccttt ggacactact
     1261 tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct taaccaggaa gtgctggccc
     1321 caggtgccgc ctttcagccg cgaacctatc ccaaagtcga tattttaaat gtgattctgg
     1381 atggagaagc agagtatcgc gacagcgaag gcaatcatgt tcaggccagc gccggagagg
     1441 cgttgctgct ctctacccag ccgggtgtca gctatagcga acacaatctc agcaaagaca
     1501 aaccgttaac gcgaatgcag ctttggctgg atacctgccc gcagcgagag aatccgctga
     1561 ttcaaaagct ggcgcttaat atggacaagc agcaattaat cgcctcgcca gagggtgcga
     1621 tgggaagcct gcaattacgc caacaagtgt ggctgcacca tatcgtgctc gacaaaggcg
     1681 aaagtgcgaa tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat
     1741 ttcatgcgct tacgcatcat gaagagaaag cggcgctaac ctgcggtgat ggggcgttta
     1801 ttcgtgacga ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag
     1861 atttgcctgt ctagttgttt ttacagggag atgatgatga gtaaaaaatc gaccaaaaag
     1921 cgccagccag cgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa aaattttggc
     1981 tatgaagaga tgttgagcga gctggaagct atcgtcgcgg atgctgaaac gcgtttagcc
     2041 gaggatgaag ctaccgcgta aagacaggtt gccggatgcg gcgtaaacgc cttatccggc
     2101 ctacaaaaca caacaacatc aacaaattaa acaccccgta ggccggataa gatgcgccag
     2161 catcgtatcc ggcatcaggt acaaaacaca acaacatcaa caagttgagc accccgtagg
     2221 ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc gtttatctgg
     2281 ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta
     2341 acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg
     2401 tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg
     2461 aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga
     2521 tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc catgccatcc
     2581 cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa cgcggtaact
     2641 ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agtcgttcat
     2701 catccgctcc gaagtgttct gctacaacca ccacaggcat tattgcagtg atcccctggt
     2761 tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg
     2821 ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc
     2881 actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg
     2941 ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca aatgggactt
     3001 cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggcgact
     3061 cttgctcgcc ctctgccaca cacgcctggt gggtaaacac cacaccattg tgcgtctcaa
     3121 tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc
     3181 agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta acagagactt
     3241 tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca
     3301 cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc
     3361 ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa
     3421 cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca
     3481 gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga
     3541 ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat
     3601 ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca aagcaacaaa ccagtgacga
     3661 taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc
     3721 acgccgggat caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca
     3781 acaccggtgc gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc
     3841 cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca
     3901 ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga
     3961 cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa
     4021 taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt accgcttcgt
     4081 catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc
     4141 caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc
     4201 gcgagcgata agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg
     4261 taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga
     4321 cgttatacag atgccacatt cccaccatcg acacgcccag cgcagcgact accagcagct
     4381 tggtcagcac catgtcggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg
     4441 ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg
     4501 tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga
     4561 acatccaaat caccagcatt acgaaataga gcgcacctaa caggatgccc cagtttttac
     4621 ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat
     4681 taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa
     4741 atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg
     4801 ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc
     4861 gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc
     4921 ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt
     4981 ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa
     5041 agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttaa aataacggag
     5101 atggataaga attttctact aaattaatcg caagagaata ttcattctct atttgcggca
     5161 gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag
     5221 ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata
     5281 tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag
     5341 gtgcggcgca tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt
     5401 tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc
     5461 tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt
     5521 aaccctgccg ctgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg
     5581 ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag
     5641 cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc
     5701 ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt
     5761 tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta
     5821 gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgaca cggcacattg
     5881 agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact
     5941 tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg
     6001 tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct
     6061 gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct
     6121 tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt
     6181 cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag
     6241 ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc
     6301 ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga
     6361 ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt
     6421 gacaacgacc cgtacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt
     6481 tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag
     6541 ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc
     6601 cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac
     6661 tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca
     6721 cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat
     6781 tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca
     6841 cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgtgcctgat
     6901 cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga
     6961 agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt
     7021 gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt
     7081 gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag
     7141 acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc
     7201 cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa
     7261 tacccatcca gcaggccaac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc
     7321 gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc
     7381 gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg
     7441 cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg
     7501 tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt gcgataggtt
     7561 ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg
     7621 cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg
     7681 ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc
     7741 gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc
     7801 gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg
     7861 ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg
     7921 taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc
     7981 tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca
     8041 aagaactgca tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg
     8101 tcgctgttga agtcagtacg catcagatcg tcattttcat actgcaagga agaggtgacg
     8161 atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg
     8221 gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag
     8281 tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag
     8341 attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg
     8401 atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag gacgccagct
     8461 ttaaagtcgc gctcaatgta gatatccagg aacgatgccg tgcgccccag cgacatcgcg
     8521 ccgccatttt gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc
     8581 tgtgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc
     8641 tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga
     8701 tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgcacc
     8761 agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacgacca
     8821 tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag
     8881 taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat
     8941 tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg
     9001 aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc
     9061 ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa
     9121 tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacctt ttcccacaat
     9181 tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat cgccttcata cggtgtatag
     9241 ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc agtccgtacc tttaaagcca
     9301 agccatgcgt cagcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat
     9361 ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac tttacctaaa
     9421 tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacgac gcaaatgaca
     9481 tgcgcatttt cactggaaac aattcgctcg ccaaaagagt tagatcgatt attcatttct
     9541 gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaaactt
     9601 gaattctctc ctattccgcc ggtgaatata attccatcca gccgacgtaa tgaagctgca
     9661 tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt
     9721 tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata
     9781 cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt
     9841 tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc
     9901 aagccttcca gcggcgtcat tcccattgag gtgtcaacac tctgaccgtt gcgaactgcg
     9961 cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga
    10021 ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa
    10081 cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga
    10141 gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat
    10201 tgctgcgccg attcaatacc acttaaattt gcgtaattat gcaggggtgc cagtggagaa
    10261 acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg
    10321 ccgccgtgag cgatgcggtg gccaattaag gccacgctgt catttaaatt ccgtttttcc
    10381 agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc
    10441 tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac
    10501 attaatactt cacaatcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag
    10561 ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta
    10621 aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt
    10681 atccagacga ccacggtatt tcgccagaga cggcgcttta cggatagcat acatcggcaa
    10741 caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt
    10801 cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgagagtatt
    10861 cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca gaatcagacc
    10921 attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag
    10981 tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttcg taccggtcat
    11041 ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc
    11101 catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca ccactgcaac
    11161 catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc
    11221 gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa
    11281 gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg
    11341 accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
    11401 gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat
    11461 cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa
    11521 cagcgccaca aagccgcgat tcagcggtgc aaatccgagc tggttttccc agaacgtcat
    11581 aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta
    11641 caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat
    11701 gttgccggaa gggttagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa
    11761 tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc
    11821 gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca
    11881 tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga
    11941 agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga
    12001 gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat
    12061 atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt
    12121 gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac
    12181 cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga
    12241 gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg
    12301 gaaagaagcc gccatgccgt gaacgttttc tgactgtacg ccaataacac gaatggtcgg
    12361 gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccaccaccac caattggcac
    12421 aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc
    12481 agcaatcact ttcggatcat cgtaaggtgg gataaaaata cgaccttcca tttcgacaat
    12541 ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga cttctgcgga
    12601 gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc
    12661 gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca
    12721 ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat taaatgcgcc
    12781 acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt
    12841 gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc gcccagccag
    12901 tcgttgtttc gcttcaataa tatcatcaat agcaaccggc agatcgtatg taatatgcat
    12961 tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca
    13021 gacaggtgga ttatttatgt ttaaataaaa acaaacaact aaccaacttc tattaattgc
    13081 cttcgtctac acccattata agatgaatac tctttggcta attccaccaa aaccgatgct
    13141 gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac aggtaatgtt
    13201 tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat atcacaagga
    13261 attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt
    13321 ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag ttcgctgtag
    13381 taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg
    13441 gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc gaacagcggc
    13501 tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt
    13561 cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc
    13621 gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt aaaaccaatc
    13681 aatgaaggaa aaccaaatga gacttccacc accgcttcag aagacatacc gcttatctca
    13741 ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg
    13801 gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct
    13861 tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc ttttgcagcc
    13921 gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga
    13981 agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc
    14041 aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt aactttcatt actttccaag
    14101 gtatcgttat tacaattaaa tatctttaaa tatcaacaag ttaaagcata aaaatcagca
    14161 taaaccctga tttttatcaa aaaaaatgac ggggataacc atattttatg gcgataacat
    14221 cattcgttat gtggttaata caaaaaaggg gctgagacca tatttcaagc aattaccgga
    14281 taattaccag gcaaaatttg agttaaattt aatgagcaag ttctcaaatt ttattataaa
    14341 taaaccattt tcagcgataa atactgcggc acgtcacatt ttttcacgct atttgttgga
    14401 gaacaaacat ttattttatc aatattttaa aatttcgaat acaggtattg atcatctcga
    14461 acaattgatt aacgtcaact ttttctcttc tgacaggacg tcattttgtg aatgcaatcg
    14521 ttttccataa attcttctcc cctcataggc gacgaatagc attttgtgtt gaggatcaca
    14581 aaacgaataa ttgctggtcg ccgcgataag gtcagacgaa gtcaacaagg gggattttca
    14641 ctgtgcttta catcagcgta ggacacagaa tgttgcttcg tttactgtaa cgccgggtaa
    14701 atgagcgttt tttgatagtg cgtaagtgtc gtgcgaggaa aataagcatc tattattgtt
    14761 tgtcatgacg acaaggcagg catcaatgaa aggcttccca attgcacata tttttcaccc
    14821 ttcaatcccg ccaatgcacg cagtggttaa caatcacaat agaaatattg attattggac
    14881 ggtaaaaaga aagtttgcag aaattgtctc caccaatgac gttaataaaa tttacagtat
    14941 aagtaatgaa ctccggagag tattatctgc aattactgca ttgaatttct atcaaggcga
    15001 tgttccttct gtcatgatcc gaatccaacc ggaaaatatg agtccattca tcatagatat
    15061 ttctacagga gaacatgatg attatatcat acaaacatta gatgtaggca cttttgcacc
    15121 tttt
//
LOCUS       DAJTNV010000092        15100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNV010000092 DAJTNV010000000
VERSION     DAJTNV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OO199_003085"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OO199_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5537044.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO199_003086"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO199_003086"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5537045.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OO199_003087"
     CDS             1533..2480
                     /locus_tag="OO199_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5537046.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OO199_003088"
     CDS             2550..3356
                     /locus_tag="OO199_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5537047.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OO199_003089"
                     /pseudo
     CDS             3366..3773
                     /locus_tag="OO199_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3773..4267
                     /locus_tag="OO199_003090"
     CDS             3773..4267
                     /locus_tag="OO199_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5537048.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OO199_003091"
     CDS             4333..5130
                     /locus_tag="OO199_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5537049.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OO199_003092"
     CDS             5141..5929
                     /locus_tag="OO199_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5537050.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OO199_003093"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OO199_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5537051.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OO199_003094"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OO199_003094"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5537052.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OO199_003095"
     CDS             complement(8372..10003)
                     /locus_tag="OO199_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5537053.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO199_003096"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO199_003096"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5537054.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEVLTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OO199_003097"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OO199_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5537055.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3592 a         3982 c         3936 g         3590 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatag cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt tagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaga agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtagaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg catggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggcgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctagtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgtacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca ggttctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt aagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcact tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgctcaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tgtcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 caggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa
//
LOCUS       DAJTNV010000093        15051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNV010000093 DAJTNV010000000
VERSION     DAJTNV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..177)
                     /locus_tag="OO199_003098"
     CDS             complement(<1..177)
                     /locus_tag="OO199_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537056.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHGL"
     gene            complement(593..1534)
                     /gene="fabY"
                     /locus_tag="OO199_003099"
                     /gene_synonym="yiiD"
     CDS             complement(593..1534)
                     /gene="fabY"
                     /locus_tag="OO199_003099"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5537057.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="OO199_003100"
     CDS             complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="OO199_003100"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5537058.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2013..2885)
                     /locus_tag="OO199_003101"
     CDS             complement(2013..2885)
                     /locus_tag="OO199_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5537059.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="OO199_003102"
     CDS             complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="OO199_003102"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5537060.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3577..4362)
                     /locus_tag="OO199_003103"
     CDS             complement(3577..4362)
                     /locus_tag="OO199_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5537061.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="OO199_003104"
     CDS             complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="OO199_003104"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5537062.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5460..6356
                     /gene="yihU"
                     /locus_tag="OO199_003105"
     CDS             5460..6356
                     /gene="yihU"
                     /locus_tag="OO199_003105"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5537063.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6380..7258
                     /gene="yihT"
                     /locus_tag="OO199_003106"
     CDS             6380..7258
                     /gene="yihT"
                     /locus_tag="OO199_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5537064.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7275..8516
                     /gene="yihS"
                     /locus_tag="OO199_003107"
     CDS             7275..8516
                     /gene="yihS"
                     /locus_tag="OO199_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5537065.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8654..9556
                     /locus_tag="OO199_003108"
                     /pseudo
     CDS             8654..9556
                     /locus_tag="OO199_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9755..11791
                     /gene="yihQ"
                     /locus_tag="OO199_003109"
     CDS             9755..11791
                     /gene="yihQ"
                     /locus_tag="OO199_003109"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5537066.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11837..13211
                     /locus_tag="OO199_003110"
                     /pseudo
     CDS             11837..13211
                     /locus_tag="OO199_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13254..14657
                     /locus_tag="OO199_003111"
     CDS             13254..14657
                     /locus_tag="OO199_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537067.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14725..>15051
                     /gene="ompL"
                     /locus_tag="OO199_003112"
     CDS             14725..>15051
                     /gene="ompL"
                     /locus_tag="OO199_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5537068.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3700 a         3875 c         3743 g         3733 t
ORIGIN      
        1 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
       61 gcaaagtgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      121 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      181 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      241 agtaaatgaa tgatactgac aatgtatgcc agcccattat ttttgcataa tatttgtgag
      301 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      361 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      421 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      481 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      541 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      601 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      661 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      721 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      781 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      841 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      901 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
      961 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1021 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1081 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1141 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1201 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1261 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1321 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1381 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1441 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     1501 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1561 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1621 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1681 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1741 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1801 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1861 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1921 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1981 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2041 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2101 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2161 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2221 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2281 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2341 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2401 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2461 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2521 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2581 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2641 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2701 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2761 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2821 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2881 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2941 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3001 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3061 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3121 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3181 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3241 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3301 cctctgcgaa cgcttcatcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3361 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3421 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3481 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3541 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3601 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3661 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3721 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3781 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3841 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3901 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     3961 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4021 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4081 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4141 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4201 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4261 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4321 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4381 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4441 gagcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4501 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4561 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4621 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4681 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4741 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4801 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4861 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4921 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     4981 ctcgcctttg gtatccacca tgatagcgga ttgcgaagat ttcgcctggt tataccgttt
     5041 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5101 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5161 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5221 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5281 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5341 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5401 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5461 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5521 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5581 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5641 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5701 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5761 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5821 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5881 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     5941 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6001 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     6061 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     6121 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     6181 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     6241 cctcacggga ggtttatagc caggcgcgcg cagcgggtca cggtcgccag gactggtccg
     6301 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     6361 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg
     6421 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc
     6481 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6541 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6601 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6661 tcctggcaac ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca
     6721 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga
     6781 tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct
     6841 ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga
     6901 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     6961 tgaaatgccg ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct
     7021 gaatgaccat atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt
     7081 cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc
     7141 agtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc
     7201 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     7261 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     7321 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     7381 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     7441 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7501 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7561 cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7621 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7681 attacaccat tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg
     7741 agtcctggga cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc
     7801 acgcggtgga agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc
     7861 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg
     7921 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     7981 cgcatcgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     8041 tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag
     8101 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg
     8161 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt
     8221 ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta caccgtcact ggcgatcgcc
     8281 agtatgaaac ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg
     8341 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg
     8401 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     8461 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8521 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8581 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8641 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8701 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8761 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8821 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca
     8881 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     8941 ctggcgagac tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct
     9001 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     9061 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     9121 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     9181 ccagcttccg gcaaactagg tttatgccgt cgatgaacac gtaaatccga caacgcttca
     9241 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     9301 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     9361 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     9421 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9481 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9541 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9601 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9661 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9721 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
     9781 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9841 tttaacccat aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga
     9901 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
     9961 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
    10021 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
    10081 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
    10141 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10201 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10261 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10321 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10381 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10441 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10501 gcccgactgg atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10561 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga
    10621 ctggtctggg atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa
    10681 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca
    10741 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
    10801 acaacacggc tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    10861 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
    10921 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    10981 tctgcccacc gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg
    11041 gcctgcgctg tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga
    11101 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    11161 ggcgggcgac cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc
    11221 ggcgctgtcg ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac
    11281 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    11341 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    11401 cggcgatgcg gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa
    11461 accttatctg aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc
    11521 gctgttcctg cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt
    11581 aggccgcgac attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11641 tctgccggag gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt
    11701 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
    11761 ggcactgttc gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg
    11821 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11881 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    11941 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    12001 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    12061 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    12121 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
    12181 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    12241 gatgaactgc tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc
    12301 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    12361 attcgtgcca gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc
    12421 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
    12481 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
    12541 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
    12601 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12661 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12721 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    12781 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    12841 ggcgttctcc tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca
    12901 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    12961 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    13021 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    13081 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    13141 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    13201 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    13261 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    13321 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    13381 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    13441 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    13501 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    13561 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt
    13621 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    13681 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    13741 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    13801 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    13861 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    13921 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    13981 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    14041 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    14101 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    14161 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    14221 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    14281 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    14341 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    14401 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    14461 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    14521 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    14581 tgattattga ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg
    14641 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    14701 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    14761 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    14821 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    14881 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    14941 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    15001 ggcttaatta atgataagag tatcagttcg ggtggtgcag tgtatctgga c
//
LOCUS       DAJTNV010000094        14941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNV010000094 DAJTNV010000000
VERSION     DAJTNV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="OO199_003113"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="OO199_003113"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5537069.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OO199_003114"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OO199_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5537070.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OO199_003115"
     CDS             complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OO199_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5537071.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2998..3081
                     /locus_tag="OO199_003116"
     CDS             2998..3081
                     /locus_tag="OO199_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5537072.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OO199_003117"
     CDS             complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OO199_003117"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5537073.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OO199_003118"
     CDS             complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OO199_003118"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5537074.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5604..7646
                     /gene="prlC"
                     /locus_tag="OO199_003119"
     CDS             5604..7646
                     /gene="prlC"
                     /locus_tag="OO199_003119"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5537075.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7654..8406
                     /gene="rsmJ"
                     /locus_tag="OO199_003120"
     CDS             7654..8406
                     /gene="rsmJ"
                     /locus_tag="OO199_003120"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5537076.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OO199_003121"
                     /pseudo
     CDS             complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OO199_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OO199_003122"
     CDS             complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OO199_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5537077.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11070..11405
                     /gene="uspB"
                     /locus_tag="OO199_003123"
     CDS             11070..11405
                     /gene="uspB"
                     /locus_tag="OO199_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5537078.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OO199_003124"
     CDS             complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OO199_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5537079.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13207..14406
                     /locus_tag="OO199_003125"
     CDS             13207..14406
                     /locus_tag="OO199_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5537080.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3719 a         3793 c         3933 g         3496 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccaaga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2281 gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat gtgtaagtgc
     2341 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaaggc ggtttgaatc
     2401 aggacgtaaa aaaaccgact ttgcgtcggt tttttactct tcagcctgag ttggtggctc
     2461 tggctggagt gagaatgccc acatctaacg gttcaatact attagccagt ggctaacatt
     2521 gcaagcgtta gcgatttctt cagctggaga agggacagtt tgctatacat ggtggtgaaa
     2581 gaggatttat atactttcat cgggtcgctg ccgtaccaag ccttgtccga atccggacgt
     2641 aaaaaaaccg acttcgcgtc ggttttttta ctcttcagcc cagagttggt ggctctggct
     2701 ggagtgagaa tgccatcatc taacgcatca atgctaatac catactgaaa ctattgcaag
     2761 gttatgctgg ttatttagcc taattacact gtatgattat ccagttagct ctgaggcatt
     2821 ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc
     2881 gttaactgat gctctggccg gagtgagatt gcccacatct aacaatgagg catgcgccgt
     2941 gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag aaaacgggtg
     3001 attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact gatccgcttt
     3061 ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc acactactct
     3121 tagcccttta acatttaacg catggtcacg aactcttctg ccgccgttgg gtgaatggcg
     3181 acggtattgt cgaagtcttt tttggttgcg cccatcttca gcgccaccgc gaagccctgc
     3241 aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc ttctggccca
     3301 acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt atacatcgcg
     3361 gtgaaagagg atttatacac tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt
     3421 tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg aatgttgctg
     3481 taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg gcgacccgct
     3541 gcaactgcca ccggtgtcag ttccactgca cccgtgttat cgcccaccgc gtaaatacct
     3601 tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt ggttttaacg
     3661 ccagcggctt cgaggttgat gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg
     3721 caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc atcggcattt
     3781 ttcactaccg ctttcgggat ggcgttggta tgcaactgtg ggccttcggc gttcatcact
     3841 tcgaccagcg tttcagagat catcgggtcg aagctgcgca gcggcgcatg tttacgcaca
     3901 aacagatgcg ttttcgcgcc gaggccgtta atcacgccag ccagctcaac ggcgatgtaa
     3961 cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa
     4021 tcaataccgt attccacgcc tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc
     4081 agaatatgat cggccgtgat ggtttcgccg ttaacttcca gcgttttggc atcaacgaag
     4141 cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc ataggaagta
     4201 tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt gaacttgttg
     4261 atggtggtat cgaaaccata atcagggccg tacatatgga tcgcttcacg gatttgcgcc
     4321 gcgtgccaca tcactttttt cggcacgcag ccgacgttca cgcaggtgcc gcccagctct
     4381 ttggcttcaa tcagtgcgca tttctggccg tacatagccg cgcggttgat ggaggcgata
     4441 ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct
     4501 tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt
     4561 actccggcac gatccagctt acggtggcgt gcccggtacc tgccggaacc agtttgctgt
     4621 gcagccacga cagcacgtta ttcatctgct gttccagctt ccacggcggg ttaatcacaa
     4681 tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagcaccgcc agttcaattt
     4741 gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat catgcgctta atttgctggc
     4801 gcagcaccac cggataccac aatgcgtaag tgccagtggc gaaacgtttg taaccttctg
     4861 ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg tcgataagga
     4921 ttaaaccacg gcgagaaacc ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt
     4981 tttcgacgcg tgcacggctg tctttctgaa atccagaacg cagcaacggg taatcgctcg
     5041 ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga gcaatcaacg
     5101 gcgaaccggg gtagtaacgc aactggccgc tacggttgaa gtgttttacc acattgatgt
     5161 acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgata ccttcgagat
     5221 attcgccggt acgctcggca tgttcgctac ctaactgata acgcccggcc cctgcgtggg
     5281 tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc aggctctgaa
     5341 cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga taactgagca
     5401 tgggtaaagg tgttccggta agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat
     5461 caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct cattgaaatt
     5521 cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc gcttccttaa
     5581 cctctttaac caggactgcg ctaatgacga atccgttact gactcccttt gaattgcctc
     5641 cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggca ttgaacgact
     5701 gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc gtacacctgg gaaaatctct
     5761 gccagccgtt ggcggaagtg gacgatgttt tggggcgtat cttctccccg gtcagccacc
     5821 tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc
     5881 tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg taccgcgacc
     5941 tgcgcgatgg cgatcattac gccacgctga acacggcgca gaagaaagcg gttgataacg
     6001 ccctgcgtga ttttgaactc tctggcatag gtctgccgaa agagaaacag cagcgttacg
     6061 gcgaaattgc tacccgtctt tctgaactgg gcaaccagta cagcaacaac gtcctcgatg
     6121 cgacgatggg ctggaccaaa ctcgttaccg acgaagcggg gctggcgggg atgccagaaa
     6181 gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc tacctgctga
     6241 cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag gctctgcgcg
     6301 aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaat gccggtaagt
     6361 gggataacag caaggtgatg gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc
     6421 tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa aacccgcagc
     6481 aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc
     6541 tggagcaact gcgtgccttc gctaaagccg aatttggtgt cgatgagttg cagccgtggg
     6601 atatcgctta ctacagcgaa aaacagaaac agcacctcta cagcatcagc gacgagcagc
     6661 tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta
     6721 tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt
     6781 tcttcgaact gtatgacgaa aataacgaac tgcgcggcag cttctacctc gatctgtatg
     6841 cccgtgaaaa caagcgcggc ggggcgtgga tggatggctg cgtaggccag atgcgtaaag
     6901 ctgatggttc tctgcaaaaa ccggtcgcgt atttgacttg taacttcaac cgcccggtaa
     6961 atggtaaacc ggcgctgttc actcacgacg aagtgatcac cctgttccac gagttcggtc
     7021 acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt atcagcggtg
     7081 tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc tgggagccgg
     7141 aggcgctggc gtttatctct ggtcactatg aaaccggcga accgctgccg aaagagttgc
     7201 tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg cgtcagctgg
     7261 agttcggcct gtttgatttc cgccttcatg ccgagttccg cccggatcag ggggcaaaaa
     7321 tcctcgaaac tctggcagaa atcaagaaac tggttgccgt ggtgccatct ccgtcctggg
     7381 gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgcggca ggttactaca
     7441 gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag gaagagggca
     7501 ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt ggcggttcag
     7561 aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc
     7621 tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca
     7681 ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga gcacgatgaa
     7741 gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag
     7801 ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca ccgacgcaaa
     7861 ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg cgattatttg
     7921 ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtact ggcttcagtc
     7981 ggctgccgcg tgcggatgct ggagcgtaat ccagtggttg ccgcgctact cgacgacggc
     8041 ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta
     8101 attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca ggtggtctat
     8161 ctcgatccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc
     8221 tttcagtcgc tggtggggcc ggatcttgat gccgatggat tactggagcc tgcacgcttg
     8281 ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc
     8341 gccacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc aggtacgcca
     8401 gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga ataattaatg
     8461 gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca tcactactgc
     8521 aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg gcaacgtctc
     8581 aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa
     8641 gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca aatgctgcgg
     8701 caccagggca gcaatcatcg ccaggccaag ggcgctaata aacaattcac ccaggctcta
     8761 gaataaatac accagcacga taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca
     8821 cattcccgca gcagcagccg tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa
     8881 tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag
     8941 tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga ccgggttgat
     9001 ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca gcgatgttgg
     9061 catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa
     9121 cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga tgatagtgac
     9181 gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca tcagccatgc
     9241 acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc tgaagctcat
     9301 cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac
     9361 cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat aaccgaatct
     9421 atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga tcgacatata
     9481 gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact tcgaaagcaa
     9541 gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca gggcgatgaa
     9601 aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag
     9661 tgctccaaga acaatggtgc gtttggtccc caacaggtgg tcgccgacat agccgccaat
     9721 ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg
     9781 cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc
     9841 gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt gctgcagcat
     9901 ccccatgggt gttgttgtat taatatttat taaccttcca ataccatcct gaaaagtatg
     9961 aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt aatattgatg
    10021 cggtaaatag attaatacac tattacaaca gaaaataacc agctgatcac actaatgatg
    10081 gaaaatatga tggtgttaaa ttttgtattg gcaggagatt ttgattgcgt acgcagatcg
    10141 taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc
    10201 ccgcatatgc gggcgttcag gaacacgtcg tggagggatg attattcttc ttcgtcgcgt
    10261 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    10321 agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc gtatttcttg
    10381 attgcatcga ccagaacctg gcctaggtcg ccgctgccgc tcagggtttc agtgattgga
    10441 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    10501 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
    10561 acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc tttctctacc
    10621 agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata agccatagtg
    10681 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    10741 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    10801 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    10861 taaacaaatt ttcgacatga atattatatt gatagtggtt aaccttctgg aaaaaaaaca
    10921 acctgatctc ctacactatc tatagagccg ctcggatgtt ggtcaccaca aaagcggttt
    10981 ctccaggacc tggtgttaac tgattggcag cgtatcggga gcggtagccc cggggtggaa
    11041 ctccgcgggc aggtcgccgg ggaggaggta tgataagcac cgtcgcatta ttttgggctt
    11101 tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc atcactacgc gcgttgttag
    11161 tggtactgcg taactgcgat ccattgctct atcaatatgt tgatggaggg ggctttttta
    11221 cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt
    11281 atcgcgatca tcacgatgat gagtttattc gccgctgtga gcgggtgcgt cggcagttta
    11341 ttctgactag cgcattgtgt ggtctggtgg tggtcagcct gattgcattg atgatttggc
    11401 attaagcact tcagcaataa taacgtataa aaaaagcggg ccagtaaact gacccgctcg
    11461 ttttggtgcg tacgattaca ggaactgcaa ggagaaccag tacagcccgc cggaaagtag
    11521 taccgcagcc ggaagggtaa acacccaggc catcagaata ctggttacgg ttttacgctg
    11581 taagccgccg ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt
    11641 ggaaaccggc atcccggtat aactcgccag gccgattgac actgccgccg tcatctgggc
    11701 agacatcccc tgagcgtagg tcatgccttt cttaccgatt ttctcaccga tagtcgttgc
    11761 cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat
    11821 ccacaccggc gcatactcga tggtgctaag catgtcggac ttcagtttct tcaacaggcg
    11881 ctgatcgtca gcactcacac caggcatctt caccactttg tcgatagtgt cagaaacgca
    11941 cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac
    12001 atcggtggtc aacatacctt tcaggcggtt gagcgcgtta atggtattcg acggatggca
    12061 gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc
    12121 ggtagcctgt ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc
    12181 atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga agcctgctgg
    12241 cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc
    12301 gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg
    12361 ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt
    12421 cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc
    12481 gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggga tattgagtgc
    12541 atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc
    12601 aatcagcgta tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat
    12661 aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag
    12721 cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa
    12781 aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg
    12841 ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta
    12901 gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc
    12961 aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag
    13021 tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg
    13081 atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat
    13141 tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt ctaagaggag
    13201 cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg tatgttctgt
    13261 tctgcgctgg caggtcaggt aggacgccgg gttctgctga tcgataatgg taaaatacta
    13321 gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa
    13381 ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc acgttttacc
    13441 cagtgggatt tcattgatct ggtcaataaa cacggtatcg cctggcacga gaaaacgtta
    13501 gggcaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc
    13561 gagaagggca atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa
    13621 acaggcttca cgcttgatct gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg
    13681 accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa gattgccgaa
    13741 caatttggcc tcaacgtgct gccgacccgc gcgggtctgg tgccattcac tctgcataaa
    13801 ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct
    13861 gaaaacggca ccgttttccg tgagaactta ctcttcaccc accgcggctt atctggaccg
    13921 gcggtgttgc agatttcaag ctactggcaa ccgggtgaat ttgtcagtat caatctgcta
    13981 ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc gaatcaaagc
    14041 ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt acagcaactc
    14101 gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt
    14161 agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa
    14221 gtgacgctcg gcggcgtgga caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa
    14281 gtgcctgggc tgtacttcat cggcgaagtg atggacgtta ccggctggct ggggggctat
    14341 aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg
    14401 aaataaccat gacgttttcc cgtcagccgt ttgtatatgt cgttattaag tcattttggt
    14461 acggttctgc atccgacatt ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat
    14521 tgccgttgtg gttattttat agtgtattta ttctcagaga atatatatta ctgaaataaa
    14581 aaacacaaag aataaccaaa ggaaattatt tgaaacgtaa aaggtatata tttttaatct
    14641 gtaaaattta aaatcacctc ctctggattg aatcaacttg ctctatttaa gacgattgtt
    14701 tttacatttt caccacctgt atcaggtggt gtttttttat tggcggcaat gcctttatct
    14761 tatttttatt acgttgtaaa tatggctatt cctaaatgaa atttgatttg ctcagtttat
    14821 ttgtgatgtc aggggagtgc ttgccatgag agtcgtgata ttattattca tcatttttag
    14881 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggtgc ttgcgtggct
    14941 t
//
LOCUS       DAJTNV010000095        14555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNV010000095 DAJTNV010000000
VERSION     DAJTNV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(334..579)
                     /locus_tag="OO199_003126"
     CDS             complement(334..579)
                     /locus_tag="OO199_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537081.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1224..1299)
                     /locus_tag="OO199_003127"
     tRNA            complement(1224..1299)
                     /locus_tag="OO199_003127"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1264..1266),aa:Phe,seq:gaa)
     gene            complement(1405..2112)
                     /locus_tag="OO199_003128"
     CDS             complement(1405..2112)
                     /locus_tag="OO199_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5537082.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2510..4645
                     /gene="speC"
                     /locus_tag="OO199_003129"
     CDS             2510..4645
                     /gene="speC"
                     /locus_tag="OO199_003129"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5537083.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4694..5950)
                     /gene="nupG"
                     /locus_tag="OO199_003130"
     CDS             complement(4694..5950)
                     /gene="nupG"
                     /locus_tag="OO199_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5537084.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6152..7231)
                     /gene="mltC"
                     /locus_tag="OO199_003131"
     CDS             complement(6152..7231)
                     /gene="mltC"
                     /locus_tag="OO199_003131"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5537085.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7296..7571)
                     /locus_tag="OO199_003132"
     CDS             complement(7296..7571)
                     /locus_tag="OO199_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5537086.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7599..8651)
                     /gene="mutY"
                     /locus_tag="OO199_003133"
     CDS             complement(7599..8651)
                     /gene="mutY"
                     /locus_tag="OO199_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5537087.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8812..9531
                     /gene="trmB"
                     /locus_tag="OO199_003134"
     CDS             8812..9531
                     /gene="trmB"
                     /locus_tag="OO199_003134"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5537088.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9531..9857
                     /locus_tag="OO199_003135"
     CDS             9531..9857
                     /locus_tag="OO199_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5537089.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10041..10760
                     /locus_tag="OO199_003136"
     CDS             10041..10760
                     /locus_tag="OO199_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5537090.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10936..11982
                     /gene="ansB"
                     /locus_tag="OO199_003137"
     CDS             10936..11982
                     /gene="ansB"
                     /locus_tag="OO199_003137"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5537091.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12099..13060
                     /locus_tag="OO199_003138"
                     /pseudo
     CDS             12099..13060
                     /locus_tag="OO199_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13161..14474)
                     /locus_tag="OO199_003139"
     CDS             complement(13161..14474)
                     /locus_tag="OO199_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5537092.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPK
                     LILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLL
                     IVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGC
                     SISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3812 a         3485 c         3740 g         3518 t
ORIGIN      
        1 gaggagtccc cagaaaagaa gacaggcata gagttttgtg atctactgat tgcgctaaca
       61 aattcaatga gatcacctct atgcctgccc gttaagtatg tcaggctttt ttccgtaatg
      121 ctctaacttc cacgcattgg tttctgacat caaataaggc tatgactttc tctccctttt
      181 aatctcattc tgatctaaaa ccatatctta aaaaaatgca tgggtatttt aattattttg
      241 atgttgatct aacgcactca aggaccattt tattaccgat tatccattct atttaactgt
      301 tgcgatataa atgtttgttt taggtgcttt aattcagtac aagtcatcaa accgtaccag
      361 accctgactg tcagtgacac cgacaggtgg gggcggcggt gtggggtcaa actgagtgtg
      421 tctgcctctg gcccacagag ccagctctgc cgccattgcc cgtggatgat actccagctg
      481 cacgttgaga ccggaaactg ggttgttgtt ctcatccacg aactgcagcg ccagccagtg
      541 catccgctgc ttaccgcagg cgacttcttt gtacttcatt ttgtgactca ggcaggtgac
      601 tgtcgtgttg cctgttgcgt ccattttgcg gttaaaccct tttaattcaa tttgttaaat
      661 tgctttccac cttttgcggg gcgcgactta accaaaatat tcactgttat cataatatgc
      721 aatgggctgg aggttgcagg tcatttaggg attttctgta agccccgttt tgcagaacgt
      781 tctatctccg attgagatcc tgtctctcgg tattaccggt agtctggcca taatccccta
      841 tggatcatat tgatatagta ggcataacac ccaaaagttg actaaagcta aatcaatcag
      901 agtgaaaaat gttcggtttg atgtaggggg ggactgagga ggggccaacg caggtggtat
      961 gcgttttact tcagaccgga caaagaccgg atgtatccgt gcagtaatga atccgttagc
     1021 caggtgctca tgtggaaata gtgtctattg gggatttgaa gtttgacaga aaaggccaca
     1081 gtgaccagaa gggggctatt tagaagaaat gttcaggctg gatggaagag gtaaagcagt
     1141 gacatcagca ttcaaagtga ccggatatct accagaaccc cagaaagcaa aaaaccagcc
     1201 cgtaggctgg ttcttctaaa tagtggtgcc cggactcgga atcgaaccaa ggacacgggg
     1261 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     1321 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     1381 actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     1441 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     1501 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     1561 cggtgtgatc agcggtaata tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     1621 taatgggata ctaattaccg acaccgcaat acccagcgag caggcgaaga tcatcgccgt
     1681 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     1741 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     1801 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     1861 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     1921 tagcgtagct aaaaccatcg ctggaaggtt ggcgcatttc acaaccagta aaataccaat
     1981 ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt ccggtaagcg
     2041 ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt tgataaaagg
     2101 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     2161 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcctta gcgaaaggtg
     2221 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     2281 tacattctct cgcttatcgc cgtttcgcgc gaaacgtttc cctttctatg ttactgctca
     2341 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
     2401 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     2461 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     2521 gaatattgcc gccagtagtg aagtggtatc ccgactttct tctcatcgcc gcgtggtggc
     2581 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     2641 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     2701 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     2761 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     2821 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctggacatca
     2881 acatggtgcg ttctttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     2941 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
     3001 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     3061 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     3121 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     3181 gattcaggcg gggacgacgc cggtctatct ggaagcttca cgtaacccgt ttggtttcat
     3241 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     3301 tgcgccagaa aaagccgacc tgccgcgccc gtttcgcctg acgattattc agctgggaac
     3361 ctatgacggc actgtctata acgcccgtca ggtgatcgat accattgggc atctgtgtga
     3421 ttacattctg tttgattccg cgtgggtcgg ttacgaacag tttatcccga tgatggcgga
     3481 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatttttg tgacccagtt
     3541 ggtgcacaaa cagcaggctg gattctcaca gacgtcgcag atccataaaa aagataacca
     3601 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     3661 tgcttctacc agccctttct atccgctgtt tgctgcactg gatgttaacg ccaaaattca
     3721 tgaaggggag agtgggcgtc ggctgtgggc tgagtgcgtt gagttgggta ttgagtcgcg
     3781 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
     3841 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     3901 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     3961 tccgtgcaag ctgttactca ctacaccagg tatcaatgcc gaaaccggcg aatatagcga
     4021 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     4081 gaagtgcgat ctcaactcca ttctgttctt attaactccg gcggaaagcc acgagaagct
     4141 ggcacaactg gtggcgatgc tggcgcaatt tgaacagcat attgaggatg actcgtcgct
     4201 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
     4261 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
     4321 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
     4381 tagcgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
     4441 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
     4501 ctggggtggg gcggttcaac gttatttcct tgcgctggaa gaaggcgtga atttgttgcc
     4561 gggattttcg ccggagttgc aaggtgtcta tagcgaaacc gatgcggatg gcatgaaacg
     4621 attgtacggt tatgtgttga agtaagaata aaaaaacggg tcaccttctg gcgacccgtt
     4681 tttctttgcg taattagtgg ctaaccgtct gtgtgcctgt cgggacacga acgtgtttat
     4741 atttgaacat cgccatgaac gcgaaggcca gaaccacgga ataaccagcg aaaatcaacc
     4801 atacggtctg ccagtcggta atgccgtttt gggtgtacat ctcaacaact ttaccgctca
     4861 cgatgccgcc gaggatacag ccgaagccgt tagtcatcat caggaacatc ccttgtgcac
     4921 tggcgcgaat tgccgggcta acttcttttt cgacaaacac cgaaccagag atgttgaaga
     4981 agtcgaatgc gcaaccgtaa acgatcatcg acagtaccag cagtacagta ccgaacggag
     5041 tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca cgccacaata ctgatcatca
     5101 ttacgttctt aataccgtag cggcttaaga agaacgggat ggtcagaatg aacagggttt
     5161 cagagatctg cgaaatcgac atgatgattg acgcatgctg cacgatgaag ctgctggcaa
     5221 acatcggatc tttgtcgaag ctgtgcagga aggtattacc gaacatgttg gtaatctgca
     5281 gttccgcgcc cagcagcatt gagaagatga agaagattgc catacgcttg tttttaaaca
     5341 gcgcgaatgc atcgaggccc agcagggttg tccagctctg attcgcttgc tgtttagcaa
     5401 ccggaatatg cggcagagtc agggtaaaca gaaccagaat ggcggaaagt gctgcgccaa
     5461 tatacagctg catgtggctt aattcgaagc cagacaggct caccacccac attgccatga
     5521 taaagccgat ggtgccccag atacggattg gcgggaagtc agtaacgata tccatcccgg
     5581 cattttgcag gcgatagtaa gagatggtgt tgattaaccc aagcgttggc atataagcaa
     5641 acgagttaat caatatcaca aggaacatcg cttccggcgt cgtgacctgt gccgccatga
     5701 acagcgtgat agcgccaatg gtgtggcaaa tggcatatac ccatttcgca cttaaccatt
     5761 tgtcggccac aatccccagc agcgcaggca taaataccgc ggcgataccc agtgagctat
     5821 aaactgcgcc aatagaagca ccgtcaaact tcagggtaac aaacatatag gagccgaggg
     5881 tcgtcagcca acttccccac agacagaact gcagaaaaga gaggattttc agctgcagct
     5941 taagattcat gttaatttcc tcacatcgtg atgcggatga gtgtttgaaa aagcacattt
     6001 gaccgtgcga gatgatggga ttctatcaac ttcgcggact ggttttttgt tacctgtggc
     6061 aaataattgc aaacattccc aatttgcaaa acgaaattgt gatgtggata acatttttgc
     6121 ccctgagcat cgtcaggggc ggttaatgga attatcggcg gcggtaggat ttttgcgcgg
     6181 tattcacttt ataaagataa cggcgagatt ccgcagaggg atggcgggtc gtcagcgtct
     6241 gataaacatc gcccggcgtc atggtgttaa taatattggc agcctgaatc ttatcattcg
     6301 aaaagactcg cagcacgctg cctgcgccgc cgttataggc ggtgatgacg gcataacgcc
     6361 gcgatgttgg gttatcaatt ccgccgagat aaacattgtt cagcatcgcc agatacgcgg
     6421 tgccggtatc aatattgctg gcaggatcaa acaagaaact gcggctcggc gtgccggatt
     6481 tcccctgcga gcggaacaca tctttcccgg cagtattttg taccacctgc attaatccca
     6541 gcgcatcgga acggctgacc gcatacgggt taaaggaaga ttcagtctgc ataattgcca
     6601 gaatcagcga ctcatcaacg ccatatttcc gtgacgcctg gcggaccatg ccgagatatt
     6661 tgtgcgcacg tttatcaagg tggttcggca ccatgttaat ggtgacgctg tagatgatac
     6721 gtagcccgtt gctgcggctt ttcagacggt ttttcagcag ataatccgcg aagttgcttg
     6781 cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac cacctgaccg taaaggaaag
     6841 gttctttcga aattgtaata tcatcaacgt cggaatagag atcgaccgaa ctcggatcgt
     6901 cacccatcag caacgttttg ataattgccc ggcgcaaatg cgcggcaggt tctgtcccgg
     6961 cgatggtttc gatagtaatc gtaccgtcat cgaagttgat gtggctgcgg gtctgatatt
     7021 gatcggtgta tttcacgtag tccttaggac cggcgatcac cacctctttg aagccccaga
     7081 tgttctcaat attgtgggca aattgcccca tcagaatatc aaaaccgttg gtatctttga
     7141 cccaggcttc gttataggta tcgccttttt tggtcgtcga acaggagatg agcaacggcg
     7201 caatcaaagc cagcgcgaga tattttttca tcattccggg tgcgtgttgt gtttgttttt
     7261 atgcaagctg ccggaggcgt gctccggcac tgtttttatt ttttatcttc cggcgtatag
     7321 ccctcgatat gcacctcttt accctcgaac aggaagttga ccatctcctg ctccagcagc
     7381 ttgcggtgct cggcattcat catgttgagt ttcttttcat taatcagcat ggtttgtttg
     7441 tgctgccact gcgcccaggc ttctttggag atctcgttat agatgcgttt tcccagctcg
     7501 ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt gcaggaaagt acaaaaaatc
     7561 gttctgctca taaatcatcc tctttatcga ctcacgcgct aaaccggcgc gccagtgcgt
     7621 aactgctgta ataaacgctc cacgggagcc gccaggccaa ctgacggcgg ttgcgctaag
     7681 ttataccaga gcgcattgcc ttcatccatg cagccggtga atgacgacac gggaagccac
     7741 ataggcacaa tatctaagtg gaaatggctg aaggtatggc gaaacgcggt cagttgcgtc
     7801 aggttatcgg cagcaatctg ccgttgtgcc agccaatgcc gcaaactttc ttcgtcggca
     7861 aactgcggga aacagtataa accgccccac aatccgctcg gcggacgctg cgccagcaat
     7921 acttcatctt cgtgctgtaa caggagaaag tagccggtgc gctccggcag cgtctgtttc
     7981 ggttttttgc ccggataaag cgaccagcta ttgttggtgg cggcaataca tccgttttgt
     8041 agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa tcatcgcgcc caaatccatc
     8101 atcgcctgat taaaccgttc cacgccgacc gcgggcgtca cctgctcgct caaactccat
     8161 agtttattct cgacctcttt tttcccaggc cagccgctta cagcatagca gcgcgccagc
     8221 acgcgtttga cgttaccgtc gagaatcgga aagtgcttac ccagagaaag cgagagaatc
     8281 gcgcctgcgg tggaacgccc gacgcccggt aacgccgcga cttcttcaaa ggtttccggg
     8341 aatttaccac cgtgtaaggt cgccacttgt tgtgccgctt tatgcaggtt gcgcgcgcgg
     8401 gcgtaatagc caagcccggt ccacaagtgg agaacttcgt cgagcggcgc attggcgaga
     8461 tcggtcaccg tcgggaagtg cgccataaag cgttcaaaat aggggataac ggtcgcaacc
     8521 tgagtttgtt gcaacatcac ttctgagagc catactttgt agggcgtctt gtcaatttgc
     8581 cagggcagcg ttttccgccc gtatttatcg taccagtcca gaacctgggc tgaaaattgc
     8641 gacgcttgca tggtcaccga cttcactgtt gttgggggca agattgcagc acagcgacgg
     8701 cagggtgtaa accggaactt tccgcagcca cggccttatc atgcttgcat ccggcaacta
     8761 actttggata atgcccgttt tcagaacact ttcacaagcg actaaatctt tatgaaaaac
     8821 gacgtcattt caccggaatt tgatgaaaac ggccgcccac tgcgccgtat ccgtagtttt
     8881 gtgcgccgcc aggggcgact gaccaaaggc caggaacatg cgctggaaaa ctactggccg
     8941 gtgatgggcg ttgagttcag tgaagatatg ctggatttcc ccgcgctttt tggccgtgaa
     9001 gcgccggtga cgcttgagat tggttttggc atgggggcgt cgctggtggc aatggctaaa
     9061 gatcgccccg agcaggactt cctcggcatt gaagtgcatt caccgggcgt tggtgcgtgc
     9121 ctggcttctg cgcatgaaga aggtttaagc aacctgcgcg tgatgtgtca cgatgcggtt
     9181 gaagtgctgc ataaaatgat tcctgacaat tcattgcgca tggtgcagct ctttttccct
     9241 gacccgtggc acaaagcgcg ccataataaa cgccgtatcg ttcaggtgcc gtttgccgaa
     9301 ctggtaaaaa gcaaactgca gctggggggc gtattccata tggcgaccga ctgggaacct
     9361 tatgcggaac atatgcttga agtgatgtct tctattgacg gttataaaaa cctgtcagag
     9421 agcaatgatt acgtaccgcg tccggcatca cgtccggtga cgaaatttga acaacgtggt
     9481 catcgtcttg gtcacggagt atgggactta atgttcgaga gggtgaaata atggcaaaga
     9541 accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga attccaggaa ttaggatttt
     9601 cggtggcatg gcgattcccg gaaggtacat cggaagaaca gattgataaa atcgttgatg
     9661 actttattaa cgaggttatc gaaccgaaca aactggcctt tgacggcagc ggttatctgg
     9721 cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg caccgaagaa catcaggcga
     9781 ttgtgcgtaa gtggctggaa gagtgcaaac tggatgaggt acgcaccagc gaacttttcg
     9841 acgtttggtg ggactaagaa agcatacggg cgatgacaaa tgcaaaactg cctgatgcgc
     9901 tacgcttatc aggcctacag agatgcacga tcgagtaggc gggataaggc gtttacgccg
     9961 catccggcat ggaaaacgcg tactttgtta tcaatctggg gccagcatat gctggcctga
    10021 tttgttcttg agggaagact atgatgcgca aaatgctgct ggcggcagca ctttcagtga
    10081 cggcaatgac cgctcacgcc gactaccagt gcagcgtcac gccgcgtgac gatgtgattg
    10141 tcagcccgca aaccgtgcag gtgaagggcg aaaacggcaa tctggtgatc acgccagacg
    10201 gcaacgtgat gtataacggt aagcaatatt ccctgaatgc cgcccagcgc gagcaggcga
    10261 aggattatca ggctgaacta cgtagcacgc tgccgtggat tgatgaaggc gcgaaaagcc
    10321 gcgtcgagaa agcccgtatt gcgctggata aaattatcgt tcaggagatg ggcgacagca
    10381 gcaaaatgcg cagccgtctg accaaacttg atgcgcagct taaagagcag atgaatcgca
    10441 ttatcgaaac gcgcagcgat ggcctgacgt tccactataa agccattgat caggttcgcg
    10501 ccgaaggcca gcaactagtg aatcaggcaa tgggcggaat tttacaggac agcattaatg
    10561 aaatgggcgc gaaagcggtg ctgaaaagcg gcggtaaccc attacagaat gtgctgggaa
    10621 gcttgggcgg tctgcaatcc tcaatccaaa ccgagtggaa aaagcaggaa aaagatttcc
    10681 agcagtttgg caaagatgtt tgtagccgcg ttgtgactct ggaagatagc cgcaaagccc
    10741 tggtcgggaa tttaaaataa tcctctattt taagacggca taatactttt ttatgccgtt
    10801 taattcttcg ttttgttacc tgcctctaac tttgtagatc tccaaaatat attcacgttg
    10861 taaattgttt aacgtcaaat ttcccatgca aagctaaggg ataatgcgta gcgtccacgt
    10921 aactggagga atgaaatgga gtttttcaaa aagacggcac ttgccgcact ggttatgggt
    10981 tttagtggtg cagcattggc attacccaat atcaccattt tagcaaccgg cgggaccatt
    11041 gccggtggtg gtgactccgc aaccaaatct aactacacag cgggtaaagt tggcgtagaa
    11101 aatctggtta atgcggtgcc gcaactaaaa gacattgcga acgttaaagg cgagcaggta
    11161 gtgaatatcg gctcccagga catgaacgat aatgtctggc tgacactggc gaaaaaaatt
    11221 aacgccgact gcgataaaac cgacggcttc gtcattaccc acggtaccga cacgatggaa
    11281 gaaactgctt acttcctcga cctgacggtg aaatgcgaca aaccggtggt gatggtcggc
    11341 gcaatgcgcc cgtccacgtc catgagcgca gacggtccat tcaacctgta taacgcggta
    11401 gtgaccgcag ctgataaagc ctccgctaat cgtggcgtgc tggtggtgat gaacgacacc
    11461 gtactggacg gtcgcgatgt caccaaaacc aacaccaccg acgtagcgac cttcaagtct
    11521 gttaactacg gtcctctggg atacattcac aacggtaaga ttgattacca acgtaccccg
    11581 gcacgtaagc acaccagcga tacgccattc gatgtctcta agctgaatga gctgccgaaa
    11641 gtcggcatcg tttataacta cgctaacgca tccgatcttc cggctaaagc actggtagat
    11701 gcgggctatg atggcatcgt tagcgctggt gtgggtaatg gtaacctgta taaatccgtg
    11761 ttcgacaccc tggcaaccgc cgcgaaaaac ggcactgcag tagtgcgttc ttcccgcgta
    11821 ccgacgggtg ctaccactca ggatgctgaa gtggatgatg cgaaatacgg cttcgtcgcc
    11881 tctggcacgc tgaacccgca aaaagcgcgc gtcctgctgc agctggctct gacgcaaacc
    11941 aaagatccgc agcagatcca gcagattttc aatcagtact aatcacctcg tcccggcatc
    12001 gtgccggggc tttttcactt cagacttacg tccattgcca attttcatta ccctaatgat
    12061 aatcaccgga ataaattatt ccgcgcgagg gttttcgggt gaaaaagcaa tggattgttg
    12121 gcacggcgct gcttatgttg atgactggta atgcctgggc agatggcgaa ccgccaactg
    12181 aaaatatctt aaaagatcaa ttcaaaaagc agtatcacgg cattctcaag cttgatgcca
    12241 tcagcttaaa aaatcttgat gctaagggta atcaggccac ttggtcagcg gaaggcgatg
    12301 tctcttccag tgacgatctc tatacctggg tcggtcagtt ggcagattac gaactgctcg
    12361 aacagacctg gacgaaagat aaaccggtaa aattctcggc gatgttaacc agtaaaggaa
    12421 cgcccgcgtc tggctggtcg gtgaactttt actcttttca ggcggcagcc agcgatcgtg
    12481 ggcgggtggt cgacgatatc aaaacgaata ataaatatct gatcgtgaat agcgaagatt
    12541 tcaattatcg ctttagtcag cttgaatctg cgttgaatac ccagaagaac tcgattcctg
    12601 cgctggaaaa ggaagtgaaa gcgcttgata agcaaatggt ggcggcgcag aaagcagcgg
    12661 atgcatattg gggaaaagat gcaaacggta agcaaatgac ccgcgaagaa gcctttaaaa
    12721 aaattcacca acagcgtgat gagtttaata aacagaacga tagcgaggcg ttcgcggtta
    12781 aatatgacaa agaggtttat caaccggcga ttgcggcatg tcataaacag agtgaagagt
    12841 gttatgaagt gccgattcag cagaaacgag atttcgatat caacgaacaa cggcgacaga
    12901 cttttctgca atcacaaaaa ctcagccgta aattgcagga tgactgggta acgctcgaaa
    12961 agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt
    13021 gatgaaaatt gacgatatca accaagctaa tgaacgctag aaaaaagata ccgaacaact
    13081 gcggcgcaat ggggtgatta agtaactcat gttaagtgcc tttgccggat aatataaaaa
    13141 gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa caaggatatt
    13201 tgcggaatgt aagtgatcat catcaacgct actagcaatg cagcataaaa tagcagcaat
    13261 ggtttgatta aatgttgaat cttcacaccc gagatagaac aaccaacaaa tagcgcacta
    13321 cccaccggcg gtgttaacag cccaatacaa aggttggcca ccatcataat accgaaatgt
    13381 actgggtcca ttcccagctc ttgtgcaatc ggcaagaaga tcggtgtaaa gattagtaca
    13441 gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat cagcagcaat
    13501 atgatcagtg ggttatcaga aatccccatc aatgcgtcgc tgatcatata aggaatatca
    13561 gcattagtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa tacaatcgaa
    13621 gtggtaacaa ccgactccag aatcagtttg ggtaaatggc gccattttac ttcccggtaa
    13681 atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc agttgcggta
    13741 aaaatgccgc ccagaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa
    13801 cttggtaagg catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagc
    13861 gggtagccac ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag
    13921 atccctggca aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg
    13981 gaaaacacaa ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg
    14041 tttacagcgg tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg
    14101 ccaccaacgg cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc
    14161 gccaggacgt tcacatgacc aagcgaacca ggaacgcggc caaccattac ctgggcgagg
    14221 ttaatcaaac gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag
    14281 gggattgcca acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt
    14341 gccgcgatat cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag
    14401 aacgaaatgg gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg
    14461 atataactat ccattggtaa tgctgccaac ttactgattt agtgtatgat ggtgatttta
    14521 aggtgcttgc gtggcttcca tttccatcag atgtc
//
LOCUS       DAJTNV010000096        14545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNV010000096 DAJTNV010000000
VERSION     DAJTNV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(91..627)
                     /gene="ssb1"
                     /locus_tag="OO199_003140"
     CDS             complement(91..627)
                     /gene="ssb1"
                     /locus_tag="OO199_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5537093.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            881..3703
                     /gene="uvrA"
                     /locus_tag="OO199_003141"
     CDS             881..3703
                     /gene="uvrA"
                     /locus_tag="OO199_003141"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5537094.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3738..4094)
                     /locus_tag="OO199_003142"
     CDS             complement(3738..4094)
                     /locus_tag="OO199_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5537095.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4098..4514)
                     /locus_tag="OO199_003143"
     CDS             complement(4098..4514)
                     /locus_tag="OO199_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5537096.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="OO199_003144"
     CDS             complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="OO199_003144"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5537097.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5740..5944
                     /locus_tag="OO199_003145"
                     /pseudo
     CDS             5740..5944
                     /locus_tag="OO199_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="OO199_003146"
     CDS             complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="OO199_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5537098.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7893..8972)
                     /gene="alr"
                     /locus_tag="OO199_003147"
     CDS             complement(7893..8972)
                     /gene="alr"
                     /locus_tag="OO199_003147"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5537099.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="OO199_003148"
     CDS             complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="OO199_003148"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5537100.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10523..11506
                     /locus_tag="OO199_003149"
     CDS             10523..11506
                     /locus_tag="OO199_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5537101.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="OO199_003150"
     CDS             complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="OO199_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5537102.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="OO199_003151"
     CDS             complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="OO199_003151"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5537103.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13448..14433)
                     /locus_tag="OO199_003152"
                     /pseudo
     CDS             complement(13448..14433)
                     /locus_tag="OO199_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
BASE COUNT         3730 a         3686 c         3484 g         3645 t
ORIGIN      
        1 agcctccata acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa
       61 aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat
      121 cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc
      181 gctgaactga ttgccaccct gcggctgctg aggttgaccc caaccgccct gcggctgacc
      241 accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat
      301 ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg attgatcggt
      361 ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc
      421 gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt
      481 catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac
      541 tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat
      601 aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata
      661 agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt
      721 ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc attccaatac
      781 tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc
      841 atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg
      901 gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat
      961 tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc
     1021 cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact
     1081 gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca
     1141 gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta
     1201 tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct
     1261 ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg
     1321 tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga
     1381 gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc
     1441 cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt
     1501 caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggaact
     1561 ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt
     1621 ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact
     1681 gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg gcgtacagca
     1741 atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat
     1801 ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca
     1861 ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt
     1921 gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac
     1981 ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga
     2041 gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc
     2101 cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc
     2161 gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa
     2221 actcgctggt cagcgggcga agattgcgga aaaaattctt aaagagatcg gcgatcgcct
     2281 gaaattcctc gtaaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact
     2341 ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg
     2401 cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct
     2461 gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga
     2521 cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca
     2581 cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc ctgaatcgtt
     2641 gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc
     2701 gaatccagaa aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac
     2761 gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt ccggtaaatc
     2821 gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat
     2881 cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata aagtgatcga
     2941 tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt
     3001 gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac
     3061 gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg
     3121 tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgcaa
     3181 aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga
     3241 agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc cagctctggc
     3301 gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg ggcagtccgc
     3361 aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg
     3421 cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga
     3481 tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata ccattgtggt
     3541 gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga
     3601 aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg
     3661 cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc
     3721 tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa
     3781 ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa
     3841 ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg
     3901 ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg
     3961 cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct
     4021 ttccagtcgt tatgcacgct ctgttctgcg cttggttttg ccatgcaata ttgtagcaac
     4081 tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg
     4141 tggatgcgat gttcccccag ccagatgcct tgccaagtgc cggtctgaat acgcccttta
     4201 tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg
     4261 tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa
     4321 cactccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag
     4381 gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga
     4441 ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc
     4501 ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg cagcaagaca
     4561 gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac tctctcccgc
     4621 tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct tgtggcaagg
     4681 gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg
     4741 cggcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta tcctgcagcc
     4801 attgcgattt tgtattttgc cctggtttat cgcccgcaaa gattaccgga ttcatgttgg
     4861 tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt ttcgtcgggc
     4921 tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca atcagctggc
     4981 gagcgacctc ttttggaatg ctgaattcat cccagccatt attcattttt tcccagaaca
     5041 caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg cgccagaatc
     5101 ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc
     5161 ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa
     5221 gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg
     5281 aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat
     5341 ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg caaaaaccat
     5401 ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt gtgatgaaaa
     5461 gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta taaaaaatgt
     5521 ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc ctcggcaaat
     5581 gagcaaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa tgatacagca
     5641 ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc ctaccctaca
     5701 atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc atatgtgaat
     5761 agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa agatatctct
     5821 tatgccaaag atttcttttt atacatagaa acacagttaa aaattgcaaa agatttttgt
     5881 agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc atttattgat
     5941 ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct ttgctgtttt
     6001 gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga tattggtaat
     6061 caatataagg ttaaacttac acaaagcgga aaaaagagtt ctctttaaca ttagaattta
     6121 acgatactat aattgaatct cagaaagtca caggcaataa ggcaaagcat attcttgagg
     6181 atatagaaat attctatcgt aacaaacccg atacttatta ttaaatttaa taatggttag
     6241 atgtgcagaa attattactc cacacgaaaa aatagggaaa ggaattccag ataattggga
     6301 taaacttaac aataaaagaa ctgacaacgt tttattatct ctcacgtaga acaacggcat
     6361 caaaaaaatc atctacaaag aaatagtgaa agcgacgaca gtttaatttc actgcaggct
     6421 gggtaactcc agcctgcttt cctgcattac atcaccgcag caaacgcctt tgccacacgt
     6481 tgtacatttg ccgtatttaa cccggcgaca cacatgcgac cgctggcgat gagatagaca
     6541 ccaaattctt cacgtagtcg gtcaacctga gcggcactta aaccggtata actgaacatg
     6601 ccgcgctgat taagcagata atcgaaattg cgttctggca tctctgtgct taataccttc
     6661 accaattcct gacgcattgc cagaatgcga gtacgcatct cttctacttc cgccagccag
     6721 ctggctttca atgcctcgtc attcagcact gcagccacca cctgcgcacc aaaattcggc
     6781 gggctggagt agttgcggcg aactgttgct ttcaattgcc ccagtacgcg gccagcggct
     6841 tcggcatctt cacacagaac agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt
     6901 ttcgagaacg aattgctcac cagagcgggt aatccagcgc tggcaatggc gcgaatagcg
     6961 taggcatcct cttccatacc ggcactaaat ccttgatagg caatatcgag gaatgggata
     7021 agctcgcggg ctttgagaat ttcaatcacc gcatcccact gatcattagt gagatcggca
     7081 cccgttgggt tgtggcaaca tggatgcagc aacacaatac tgcgggcagg taatgttttc
     7141 agcgtcgcca acaggtcatt aaagcgcacg ctgttagtca cttcgtcata ccaggggtaa
     7201 gtactcactt cgaatccagc cccggcgaat attgctacgt ggttttccca agtaggatcg
     7261 ctgacccaga cgcctgattc cgggaagtag cgtttcagga aatccgcgcc cactttcaat
     7321 gcccctgagc cgccaagggt ttgaatggtt gctacgcgct gttgttgcag taccggatgg
     7381 tccgcaccaa acagcagcgg cgcaatggca tggcgatagc tgttaagccc ttccatcggt
     7441 aaataaagcg aagcgccatg gggctgcgca ttcaggcgcg cttccgcctc cgccacggct
     7501 ttcaattgtg gaataattcc gtcttcgttg tagtacagac cgatacttaa attcactttg
     7561 tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc gccagcgtag
     7621 gcgtcaactt tttgaaacac gcgatggttc tccaggttta cgggcaggtg gttaaaacac
     7681 aataaaccgg aagaaggcga agatcgagtg gatgttcagg agcgaacggc aattagcaac
     7741 agagtgagac taatgataaa cgtacatccg ccagaggcac gacctttata agagcatgaa
     7801 agaacatcaa cttattgaat ttttaggatt ttattggccg gataaggcat tcacgccgca
     7861 tccggcacag acaatcaaat attacagaac gattaatcca cgtatttcat cgcgaccctt
     7921 gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg tcatttcagc gatacgttct
     7981 acgggcaaac cttcgcccca taaaatgacc ggatccccgg ctttgtcctg cgcctgtgga
     8041 cctaagtcta cgcagatcat atccatcgcc acgcgcccga caatcggtac ttcgcgaccg
     8101 ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc atagcccatc
     8161 gcgactacgc caagacgggt atcacgttcg cttacccagg ttccaccata accaacaggc
     8221 tctccggctt tatgctcacg cacggcaatc aggctggagg ttagtgacat cactggctga
     8281 cagccaaaat cggcaccggt ggagcgatct tccagcggcg agacgccata aagaatgatg
     8341 cccgggcgca cccagtcaaa atgcgactgt ggccacagca gaatgccacc cgacgcggca
     8401 atggaacgtt gaccaggttt gccttcgcaa aaggtattaa agatagcgag ttgtttctcg
     8461 gttgcgccac attttggttc atccgcgcgc gcaaaatggc tgacgatatt caccggctga
     8521 cgaacgtttt tgcactgggt caggcgatga taaaacgcct cagcctgttc cggccttacg
     8581 cccagacggt gcataccggt atcgagtttc atccagacgg taaccggctc gtccaagcta
     8641 gcctcttcca gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca
     8701 gaaatcgtcg gcagatctct ggcatcaaaa aagccttcga gtaacagtac aggtttggtg
     8761 attccccccg cacgcagtcg cagagcttct tcgagacggg ctacgccaaa ggcgtcagca
     8821 tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc
     8881 gcaaccattt tactggcagg tgccagttca cgaagacgtt gcaggttgtg tcgcagagcg
     8941 cggcggttaa tcacaacagt tgccgcttgc atttgtgttc cttgataatt gtttgcttta
     9001 attacctaat tcataaaata attattattc gtcgtcgtac tgcggccccg catagttgtc
     9061 gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg
     9121 tttaccgata ataatttccg cgatgccttt taaatcactg ttttcgtgat acacctcatc
     9181 acgatagata aacatgatca agtccgcatc ctgctcgata gagccagatt cacgcaggtc
     9241 ggagttgacc ggacgtttgt cggcacgttg ttccagagaa cggttcaact gggacagcgc
     9301 caccaccggc acgttcagtt ctttcgccag tgctttcagc gagcgggaga tttctgcaat
     9361 ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat
     9421 gataagcccg atgccgccgt gttcacgggc aatacggcgt gcgcgggaac gcacttccgt
     9481 tggcgtcagg ccggaagagt catcgatata gatattgcgt ttttcgagca aaatacccat
     9541 ggtgccggaa atgcgcgccc agtcttcatc atcgagctgc ccggtacgga ttttagtctg
     9601 gtcaacgcgc gacagcgacg ccagagaacg catcatgatc tgttctgatg gcatctccag
     9661 cgagaagata agtactggtt tatcctgcaa cattgccgcg ttttcgacga ggttcatcgc
     9721 aaatgttgtt ttacccatcg acggacgcgc ggcgacgatg atcaaatccg acggctgcaa
     9781 gccagcggtt tttttgttga gatcgtcata accggtgttt accccggtaa cgccatcgtg
     9841 tggctgctga aacaactgct caatacgcgc cacggttgcg tcgagcacat cggcgatgtt
     9901 cttcggccct tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc gggattcagc
     9961 aaggtccagc agatcttcgc tggtacgccc ctgcggatca aaaccggctt cagcaatctc
    10021 attcgcaacc gagatcatct cacggacaac ggcacgttca cgcacgatgt ccgcataagc
    10081 actgatgttc gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc
    10141 gacgctatcg agttgcccct ggcgttccaa cgattccgca agggtaatca gatcgatagg
    10201 actaccgctt tcctgcaaac gcgccatttc agtaaagata tgacggtgtg ggcgggtgta
    10261 aaaatcgtct gctaccacac gctcggctac atcatcccag cgttcgttat ccagcattaa
    10321 accgcccaac accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac
    10381 ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat
    10441 agtgaatgga gttaccgaaa taaagaatgg gtcgaaactt taccatatga agcagaccct
    10501 gacgatacgt tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc
    10561 ggaagtactt caagccgtag agttcactcc tgccgatccg gcggagaatg aaatccaggt
    10621 cgaaaataaa gccatcggca tcaattttat cgacacgtat atccgcagcg gcctttaccc
    10681 gccgccatcg ctacccagcg gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg
    10741 cagtggtgta aagcatatta aggctggcga tcatgtagtc tatgcgcagt cggcgttagg
    10801 cgcttacagc tctgtgcata acattaatgc ggataaagcg gcgattctgc ctgcggcaat
    10861 ttcttttgag caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg
    10921 caaaacctat gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcat
    10981 tggcttaatt gcctgccagt gggcaaaagc cctgggcgcg aaactcatcg gcaccgtagg
    11041 aaccgcgcaa aaagcgcaga gcgcgctaaa agccggcgcg tggcaggtta ttaactatcg
    11101 tgaagagaat ctggtcgagc gattgaaaga gatcaccggc ggtaagaaag tgcgcgtagt
    11161 gtacgattcc gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg
    11221 cttaatggtc agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct
    11281 caatcaaaaa ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg
    11341 ggaggaatta accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa
    11401 ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat
    11461 tctggaaagc cgggcgacgc aaggttccag cctgctgatt ccataaaaga aatagggctt
    11521 ccacctggga agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat
    11581 ctgttagagg cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa
    11641 aaccttaggt tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat
    11701 atttcggcat ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta
    11761 gtaaccacgg cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg
    11821 tcgccacaac cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga
    11881 caaaaaagcc aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct
    11941 tgctggcatt acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg
    12001 cgacggtgcg cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc
    12061 gccaccagtt tgagcgcgtg ttccagcacg ttaatgtcag cacccgcttt atgggcattt
    12121 tcacttaaat aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa acccagcata
    12181 tgccggataa tatggccgag atacgtcccc tggctgagtt catgctcaat gtacggatac
    12241 atggcacgca ctactgccac cggatcggca tcggtatccg aggaaccaaa gatctcccgg
    12301 tctaccgccg ccagaatacc cggattctga tacgcctcgc gcccgaccat cacgccatcc
    12361 atatgttgca aatgcgcttt agcttcttcc agcgacttga taccaccgtt aatcgacatt
    12421 gtcagatgcg ggaagtcacg cttcagttga tacacacgcg gataatcgag cggcgggatt
    12481 tcacggtttt ctttcgggct taaccccgaa agccaggctt tacgtgcgtg gatgatgaac
    12541 atctcacact cgcctttgcc ggaaacggta ttgatgaaat cgcagagaaa ttcatagctg
    12601 tcctggtcgt cgatgccaat acgcgttttc accgtcaccg gaatcgacac cacatcgcgc
    12661 atcgctttca cgcagtcggc aaccagctgc gcattaccca tcagacacgc accaaacatg
    12721 ccgttctgca cccggtcaga cgggcagccg acattcaggt tgatctcatc atatccgcgc
    12781 gcttctgcca gctttgcaca ctgcgccagc gccgccggat cgctaccgcc cagttgcaac
    12841 gctaccggat gttcttcttc actgtacgcc agataatcac ctttaccgtg aataatcgcc
    12901 cctgtggtca ccatttcggt ataaagcaac gtattgcggg aaagcagacg caagaaatag
    12961 cggcaatgtc tgtccgtcca gtcgagcatt ggtgcaacac taaaccgacc actccagtga
    13021 acgtcagttt tttcaggcat tgcgctggtt tggttgattt tttgcatttc agaattaccg
    13081 tgcattttca aatgtagaga ttattttatc gatatatcat ggggttatgt tattcagctt
    13141 cactgttcag gaggctcaat agcggggtac tataccataa caacaggaag cgcctgtctc
    13201 attgcaaaag aaaattgaga tcctctcaag gcatgaagct ctcacgaagt gatggaaata
    13261 atcttattag ccgttaactt tgttaaggcc aatgataaac aatccaggtt cgacgataaa
    13321 taaaaaatca cacattaaac tctggtgata tatctccctg ctaatagcat tgatagagaa
    13381 aaaagaaccc aataaatatt gggtcctttt atataatgcc ttccatacta tcgaagaact
    13441 tcacatatta tttctccgat ttaaccccga acaagtcata aattaattta gaagtgtctg
    13501 taagtatttt aatctcttcc tttgaggttg ggtcaaacga cttcgcgaag ctaattaatt
    13561 gtggtgcagc atctcgcatt ttgcttaaaa tatcaggatc gagcgttcct tcattcacca
    13621 gatgtgacat cttatccaga tagccatcaa aattcatttc tcccccatct gccagacgct
    13681 tcatctctcc caggtacttc ttcatatcac gttgatataa tttttcaaac tgagctttca
    13741 ggtagctctc attattttca ttaatataca ctgtttctga taaatctgcg taggcactag
    13801 aatatgaaag tgaaattaac agcgaagcaa ataattttaa gctgttgtca tcatgttcct
    13861 ggcaggcatt aacaaacgtt aaaaaaccag aacctattgc ttgaaaatgt atattcgcta
    13921 aaggttcatt atccttagat tcctcataaa atagctgaac cgtggaggga aggccattct
    13981 tagtttctac atcaaaagta cattgttcaa tgggcattgc atctttatta tccccaggat
    14041 tacatatact attaatcgcg aaatgaaaaa tagcccggtc aacaactgat gcaataaagt
    14101 cacgatcgtt taaatcctca tcagtgcaat cgtcagtata cttattgacc cacattttga
    14161 gatcatttat gtgctcaatt aaaaacacat ttttttacgt gcatgccata aattatgcgt
    14221 gattcgttga ataaacaaat ctatttttgc aataattata tcccgatgac gcagcgctgc
    14281 ataatcaata gatttaccat atatattatt taatttacct ttgattgaac ctctattatc
    14341 caatttatct ttggttgcat ctttattttc gaattgattt gaattgagtg ttttattaaa
    14401 gtatccagtt gcaccgggta ttatcttcaa catgattcat ctaccttttt tgtcgtaccg
    14461 tgtcctcctt ataattcttt tgccatctgg tcactatcaa aaatcgctca taatttctga
    14521 actacaggta tgcgtttaaa cccag
//
LOCUS       DAJTNV010000097        13956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNV010000097 DAJTNV010000000
VERSION     DAJTNV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="OO199_003153"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="OO199_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5537104.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="OO199_003154"
     CDS             complement(1604..1777)
                     /locus_tag="OO199_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5537105.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="OO199_003155"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="OO199_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5537106.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="OO199_003156"
     CDS             3415..3744
                     /locus_tag="OO199_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5537107.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OO199_003157"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OO199_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5537108.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OO199_003158"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OO199_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5537109.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OO199_003159"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OO199_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5537110.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OO199_003160"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OO199_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5537111.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OO199_003161"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OO199_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5537112.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OO199_003162"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OO199_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5537113.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="OO199_003163"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="OO199_003163"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5537114.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3244 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAJTNV010000098        13808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNV010000098 DAJTNV010000000
VERSION     DAJTNV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..205
                     /locus_tag="OO199_003164"
     CDS             <1..205
                     /locus_tag="OO199_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537115.1"
                     /translation="FGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNK
                     TCITSDCWQILFFSPDHFNNDFY"
     gene            227..574
                     /locus_tag="OO199_003165"
     CDS             227..574
                     /locus_tag="OO199_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537116.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            743..1393
                     /locus_tag="OO199_003166"
     CDS             743..1393
                     /locus_tag="OO199_003166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537117.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(1901..2277)
                     /gene="rnpB"
                     /locus_tag="OO199_003167"
     ncRNA           complement(1901..2277)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO199_003167"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2310..3455)
                     /gene="garK"
                     /locus_tag="OO199_003168"
                     /pseudo
     CDS             complement(2310..3455)
                     /gene="garK"
                     /locus_tag="OO199_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(3552..4442)
                     /gene="garR"
                     /locus_tag="OO199_003169"
     CDS             complement(3552..4442)
                     /gene="garR"
                     /locus_tag="OO199_003169"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5537118.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4472..5242)
                     /gene="garL"
                     /locus_tag="OO199_003170"
                     /pseudo
     CDS             complement(4472..5242)
                     /gene="garL"
                     /locus_tag="OO199_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(5258..6592)
                     /gene="garP"
                     /locus_tag="OO199_003171"
     CDS             complement(5258..6592)
                     /gene="garP"
                     /locus_tag="OO199_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5537119.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6967..8538
                     /gene="garD"
                     /locus_tag="OO199_003172"
     CDS             6967..8538
                     /gene="garD"
                     /locus_tag="OO199_003172"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5537120.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(8590..9414)
                     /gene="agaR"
                     /locus_tag="OO199_003173"
     CDS             complement(8590..9414)
                     /gene="agaR"
                     /locus_tag="OO199_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5537121.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            9663..10943
                     /gene="kbaZ"
                     /locus_tag="OO199_003174"
     CDS             9663..10943
                     /gene="kbaZ"
                     /locus_tag="OO199_003174"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5537122.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10966..11439
                     /gene="agaV"
                     /locus_tag="OO199_003175"
     CDS             10966..11439
                     /gene="agaV"
                     /locus_tag="OO199_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5537123.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11450..12229
                     /locus_tag="OO199_003176"
     CDS             11450..12229
                     /locus_tag="OO199_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5537124.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            12219..13028
                     /locus_tag="OO199_003177"
     CDS             12219..13028
                     /locus_tag="OO199_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5537125.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            13115..13549
                     /locus_tag="OO199_003178"
     CDS             13115..13549
                     /locus_tag="OO199_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5537126.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13546..>13808
                     /locus_tag="OO199_003179"
     CDS             13546..>13808
                     /locus_tag="OO199_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5537127.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSW"
BASE COUNT         3510 a         3557 c         3351 g         3390 t
ORIGIN      
        1 ttttggtgaa caatgtactt gctcagccgt caataaaaaa gagctggaat gtattaagga
       61 gacgatttct aagtattgcg caaagttcac ccaaaaagaa gccatcttaa caccccctag
      121 gcactttaat aaaacctgca ttacttcaga ttgttggcaa attctctttt tttcacccga
      181 tcattttaat aatgattttt attgattatt tataaaggat aaaataatgt tccctgtttc
      241 atcaattggt aatgatatta gtagtgattt agtccgtcga aaaatgaatg accttcctga
      301 aagccccata ggaaataacc tcgaagcact agcaccaggt atagaaaaac taaaacagac
      361 ctctattcaa atggtcactt tacttaacgc gttacaacca agtggaaaat gcattatcac
      421 tggtgatttt caaaaagaat tagcgtactt acaaaatgta attctttata atgactcgtc
      481 tcttcgtctg gattttttag gttataacac ccaaattatt caacgatcgg acaatacctg
      541 cgaacttacc attaatgaac cgttaaaaaa ctaggaaata tctacaggca atatcaatgt
      601 taattgccca ttaaaagata tttacaatga aatcaggagg ttgaacgtaa tctttagttg
      661 tgggactgga gatatagttg atctatcctc tctggactta cgtaatgtcg atttagatta
      721 ttatgatttc acagataaac atatggctaa tactattcta aatcatttta aattggattc
      781 aacaaatttt actaatgcca acatgtttca ggttaatttt gttagttcaa cacaaaacgc
      841 cacaatctcc tgggattatt tactaaaaat aacgcctgtt ttaataagca ttagcgatat
      901 gtattctgaa gaaaaaatca agtttgtcga aagttgttta aatgagcttg gagacattac
      961 cgaagaacaa ttaaaaatta tgagatttgc aattataaaa tctataccaa gggcaactct
     1021 tacagataaa ttagaaaatg aattaacaaa agaaatatat aaaagctcat cgaaaatcaa
     1081 caattgcttg aacagaatta aattaccaga gatgaaagaa ttctcatcaa aaaaaatata
     1141 tgattacatc gatacaatca ttgaagatta tgaaaatatc aaagaaaatg cttatctggt
     1201 cgtcccccaa attaattata ctatggattt aaacatagaa gactctagct cagaagagtt
     1261 aataccctcg agaaagacga aaattctccg gacaatggct ttgaggtcgg ggaatataac
     1321 acatatgaag catataactc agagaatcaa tattttacca gagaggacta tacgtataat
     1381 tacgaccttt taaatgcaat atagacttaa taacttctta caaagtctat tattaatacc
     1441 gtagatattc atcttaccct gtgcaccaaa agcaaaaact tatcgacgcg ggtttgaaac
     1501 tgatggcaac cgcaaaaatg cctgatgcgc tacgcttatc aggcctacgc catctctgca
     1561 atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgcagc atccggcatg
     1621 aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta catcaggtga
     1681 aactgaccga taagccgctt tcttttgggt atagtgtcgt ggacagtcat tcatctttct
     1741 gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtatatcagg tgaaactgac
     1801 cgataagccg ctttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc
     1861 caaaagcaaa aacccgccga agcgggtttt tacgtaaaac aggtgaaact gaccgataag
     1921 ccgggttctg tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag
     1981 cctacccggg ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg
     2041 gagtttaccg tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca
     2101 cccttacctg atcccgcttg cacgggccat cggcggtttg ctctctgttg cactggtcgt
     2161 gggtttcccc cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc
     2221 tccgcccgtc tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc
     2281 gcgcagtata gagggtttgc gcgcccttgt caccccgtgt tgcgcattcc aatcgccagt
     2341 gtcgcggcaa tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg
     2401 tccaacgtac ctatgctggt caatacgctg aagaccgcat caatgccatg ctgatgtaca
     2461 acgccaacat catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc
     2521 gccacgtttg cgacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct
     2581 tcaccggtga tcaccagcgt acaatcgtga atatgttcct ccagattcag cgccgtagtg
     2641 acgatttcaa taccactttt cagttccgca ccaagaaacg ccatcagcac cgcacccata
     2701 ccgcccgcag ctcctgcgcc ggggacatct tttacatcaa catgcagcgc ttttttaatg
     2761 acatcggcat agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc
     2821 ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca
     2881 caagcgacgc gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca
     2941 ttcagagtat taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat
     3001 ttcgccccca gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct accgccaatg
     3061 ccgataataa tgtttgtcgc gccgctctcc agcgcctaca ggattaactc gcctgtgccg
     3121 cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc
     3181 gccgccattt caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact
     3241 ttctcgccca gcggccctgt aacccaggcg tgacgttcag aaccctgggt ggctgcaatc
     3301 atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca
     3361 ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt
     3421 tctttataag agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc
     3481 cggagggcgt ttctcttatt cggcctggat tccaggcccg gattgcaata cgccatccgg
     3541 gcacgacgtc attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacgccagg
     3601 gcgctatgat ccgccgttcc taaaccatct gctcgcagtg cctgcatcat ctccataacc
     3661 gcagctgtga gcggcagttg cgcgccgacg ccgtgggaag tatccagcgc atttgccaga
     3721 tccttaatat gcagatcaat acggaagccc ggcttgaagt tgcggtccat caccatcggc
     3781 gctttggcat ccagcacggt actgcccgcc agtccaccgc gaattgcctg atacaccagg
     3841 tccgggttaa cgcccgcttt agttgccagc gttaacgctt ctgacatcgc ggcaatattc
     3901 agcgccacaa tgacctgatt cgccagtttg gtgacgttac ctgcaccaat tgccccggta
     3961 tgcaccacgg aacccgccat tgctttcatc aaatcatagt atttgtcgaa aatagccttg
     4021 tcgccgccca ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc
     4081 ggagcatcca gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc
     4141 agcggtgcga tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg
     4201 ccattctcac ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg
     4261 acgtcgcact gttcagcgat cgctttagcc gtagaggctg tttctgcacc tgcagcaatc
     4321 acgtcagcaa tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga
     4381 aggtttttac tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc
     4441 atatcaatca tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttat
     4501 gagtggcaga gcggaagacg ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt
     4561 ccagataacg acgcgcatcg gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg
     4621 cgctggcacg gttaaaaatg tgctgaattg ctttttgtac atccgggtgt gatgcattgc
     4681 cgagatggcc taatgccgcg gccagatcgc tggggccgac gaagatgccg tctacgcctt
     4741 cggtagcggc aatggcatcg acgttatcta cgccctgctg actttctatc tggaccagaa
     4801 tagtgatgtt cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc
     4861 ggtgagaaac ggagacgccg cgaatgcctt ccggtgggta acgggttgat gccaccgcct
     4921 gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca
     4981 gaagacgctt aataatttcc ggctcgttgg tcggcactca cactactggc gcgctggcgc
     5041 tgcctttcaa ggccattaac tgcggaataa acgtggagat atcgtttggc gcatgttcgc
     5101 catccagcac cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag
     5161 agagtgctga ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat
     5221 tcgggaaaac atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt
     5281 taatgtcgcc aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga
     5341 aaaccagtgc tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag
     5401 tgacaatgga ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct
     5461 ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa
     5521 aagccagcgc catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga
     5581 tggtggaagc cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata
     5641 aaccgcgttt gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac
     5701 acagtgccgg aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca
     5761 ccagataaat cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc
     5821 cgaaaaatac gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttga
     5881 gaccgcttgc tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt
     5941 cagagataaa cttcagctct tcctcagaca tacgtgggtg atctgtcggg ttatgaatca
     6001 acttgatcca cagcgccgtc agcacaaaac aaatcacccc cataacggta aagacgtgct
     6061 cccatcccca ggcgaaagtc agccagccaa gcagaggcga aaagagcgcc agcgagaaat
     6121 attgtgccga gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg
     6181 cgacaatacg ggcgttcgcc gggaatgatg gagcttccga gaatcccagc ataaagcgca
     6241 taaagaacat ggagatccct gcccaggcca gcgggaacat atcaacaaag ccttgcagga
     6301 aggtgaacag cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat
     6361 caagcaacca gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcggagaaga
     6421 tgtaacccat cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag
     6481 acagcgttgc acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta
     6541 aatagcgggt atgcacacct ttcctttttt cgtcaacggt gtccagaatc attttattta
     6601 cctcgggtac ttatgctgat ttttattatt atggggaagg tgttatttat aagtttcatt
     6661 tatgccgtaa cgacaatgaa ctcgggaatt agtataagca gcgcgagaat aataatcatt
     6721 gtgcaaatgc taatttaatt aatacttttt aaatattatt ttgagcatat gcacataagg
     6781 ttgcgcgcta aagcacagat ttgcgcttta ccttgccggg cggcactgca atccctgaaa
     6841 tgattgacat tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga
     6901 aatcatttat ttttaaatga gaccaggtcc tcattttaat aacccctggc tggagaatat
     6961 tgcacaatgg ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt
     7021 cacgacacag ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt
     7081 tttccggatg ggctggaatt aattgaacat attccccagg ggcataaagt cgcattgctg
     7141 gacattccgg ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgt
     7201 gcaatcccac gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg
     7261 ttacacacgc tgccactggc aaccaaagtc ccggaaccct taccgccgct ggaaggatac
     7321 acctttgagg gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc
     7381 accaccagcg tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc
     7441 gatctgctac cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggt
     7501 tgtggcgtgg cgattaacgc accggcagca gttgtaccta ttcgtaccat tcacaatatt
     7561 tcgctgaacc ctaactttgg cggcgaagta atggtgattg gcctgggttg tgaaaagttg
     7621 cagcctgagc gcctgctgac tggaacggat gatgtgcaag ctattccagt agaaatcgcc
     7681 agcattgtca gtttgcagga tgaaaagcat gtcggttttc agtccatggt cgaggatatt
     7741 ttgcaggtcg ccgaacgcca tctacaaaaa ctgaatcaac gtcagcgaga aacctgtccg
     7801 gcttcagaac tggttgtcgg catgcattgc ggtggcagcg atgcgttttc tggcgtaacg
     7861 gcaaacccgg cagttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg
     7921 ttttcagaag tcacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac
     7981 gaagaggtcg gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg
     8041 ggaaaaaccg accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaac
     8101 gtggtagaga aagcgctcgg ctccattgct aaatcgggca aaagtgcaat tgttgaagtg
     8161 ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc
     8221 gattttgtct gtggcacgca acaggtggct tcgggtatca ccgtgcaagt gtttacgacc
     8281 ggtcgtggta cgccgtacgg cctgatggcg gtacccgtca ttaaaatggc aacccgcacc
     8341 gagctggcga accgctggtt tgatttaatg gatattaatg cgggcaccat cgctaccggc
     8401 gaagaaacta ttgaagaggt gggctggaag ttgttccact ttattctcga cgtcgccagc
     8461 gggaagaaga aaaccttctc ggatcaatgg ggactgcata accagctggc ggtgtttaac
     8521 ccagcaccgg tgacctgata attcaattcg tattatgccc gatagcacac gtttatcggg
     8581 catattgagt cacagcgaag acgcgtgatc cccgaccaga atcacttcaa ccccagcctt
     8641 tcgcagtcct tccagactat ccgcaggaat gccttcatca acaatgatca tgtcgatacg
     8701 ttgagtatca atgatcttat gtaaactgga acgattgaat ttactggaat cggtgaccac
     8761 gatgatccgt tccgcaactt cgcacatccg acggtttaaa cgggcttcat cttcattatg
     8821 tgtgctgacg ccgcgctcca gatcgatcgc atctacacca agaaacagca tatcgaagtg
     8881 gtaattttgc agcgattgct cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa
     8941 atgcccgccg gtcatcagca gctcaacgcc ttccgcttcc agcaacgcat tagccacgtt
     9001 cataccgttg gtcatcgcaa ttacgtcagt gtacttgcgc atcagacgag caatctcaaa
     9061 agtggtggtc ccggaatcga ggatcacccg atgacctggc tgaatcaact caacggcagc
     9121 tttcgcaacg ctgcgtttca tcgcggtgtt cagtgcgctt ttatcttcca ctgatggctc
     9181 gactgacggc gtcgtgctat cgcagatcaa cgcgccacca taggcacgca cagcgatccc
     9241 ctgcttttcc agaaacgcca gatcattgcg gatcgtcaca gtagatacgc catacaatgc
     9301 cgacagatcg ttaacctgca cactcccttg ctgtcgcaga cgctgaatga tctgttctcg
     9361 tcgctcgctg gtgcctgtca ctcgcttctc acctgaagcg tcggtattac tcatagtaag
     9421 tcctttcgta aaactttcgt ttcatttcgt tttgcctatt aacgcctttc tattaagcaa
     9481 atgcaagccc accttgccca ttggcgcaag ctactctcgt ttcagtgact ttcattacgt
     9541 ttcttttgtg aatcagatca gaaaactatt atctttcgtt ttatttttat ctcaccatga
     9601 cgcagtatca attgaaacaa aacgaaagat caatatcgca gtaatctgaa ctggagagga
     9661 aagtgaaaca tctgacagaa atggtgagac agcacaaagc gggcaaaaca aatgcaattt
     9721 atgccgtttg ttccgcacat ccgctggtgc tggaggctgc aatccgctac gccagtgcaa
     9781 accaaacgcc gttactgatt gaagcaacct ccaatcaggt agaccagttc ggcggttata
     9841 ccggaatgac gcccgccgat tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt
     9901 tcccgcagga tgcgctgatt ctgggtggtg accatctggg gccaaaccgc tggcaaaacc
     9961 tgccagccgc tcaggcaatg gccaatgccg atgatttgat taaaagctac gttgcggcag
    10021 gattcaaaaa aatccacctt gattgcagca tgtcctgtca ggacgatcca attcccttaa
    10081 ctgatgacat cgtggctgaa cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc
    10141 gcgaacactt tggcgaagcc gatctggagt atgtcattgg taccgaagtg ccggtacctg
    10201 gcggcgcgca tgaaacctta agcgagctgg cggtcaccac gccggatgcc gcccgcgcca
    10261 cgctggaagc ccatcgtcac gcctttgaaa agcaaggttt gaatgccatc tggccacgca
    10321 ttattgccct ggtggttcaa cccggcgtcg aattcgatca caccaacgtt attgattatc
    10381 agcccgccaa agcgagcgcc ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg
    10441 aagcgcactc taccgattat caaacgccgc aatctctgcg ccagctggtg attgaccact
    10501 ttgccattct gaaagttggc ccggcgctga ccttcgccct gcgtgaagcc cttttttctc
    10561 tggcggcgat tgaagaagaa ctggtaccag cgaaagcctg ttccggtctg cgtcaggtgc
    10621 tggaaaacgt gatgctcgac cgcccggaat actggcaaag ccactaccac ggtgacggca
    10681 acgcgcgtcg tctggcgcgt ggttacagct actcagatcg cgtgcgctat tactggccgg
    10741 acagccagat tgatgacgct ttcgctcatc tggttcgtaa tctggcggat tcaccaattc
    10801 cgctaccgct catcagccag tatctgccgc tgcagtacgt gaaagttcgc tccggcgagc
    10861 tgcaaccgac gccacgggaa ctcattataa accatattca ggacatcctg gcgcagtacc
    10921 acacagcctg tgaaggccaa taagcaaaac aaagaggaac atgctatgcc aaatattgtt
    10981 ttaagccgga ttgatgaacg cttgattcac ggtcaggtcg gcgttcagtg ggtcggattt
    11041 gcgggggcaa atctggtgct ggtagccaac gatgaggttg ccgaagatcc ggtacaacaa
    11101 aacctgatgg aaatggtact ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa
    11161 gttatcgaca acattcatcg cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca
    11221 cccgccgatt tcctgacgct ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc
    11281 aatatgcact acgccaatgg caaacaacaa atcgccaaaa cggtttctgt ggatgcgggc
    11341 gatatcgcag catttaacga cctgaaagcc gctggggtgg aatgcttcgt tcagggcgtc
    11401 ccgacagagc ctgctgtgga cctctttaaa ttactttgag ggattcatca tggaaatcag
    11461 cctgttgcag gcatttgcgt tgggcattat cgcctttatc gctggcctag atatgtttaa
    11521 cggcttaacc catatgcacc gcccggtggt cctcggcccg ttggtcgggc tggtacttgg
    11581 cgatctgcat accggaattt taaccggcga tacgctggaa ctagtgtgga tggggctggc
    11641 cccgctggcg ggcgcacagc cgcctaacgt gattatcggt actatcgtcg gcacggcgtt
    11701 tgccattact actggcgtga aacccgatgt cgcggtcggt gtcgccgtac ctttcgctgt
    11761 cgcagtacag atggggatta ccttcctgtt ctcggtgatg tctggcgtga tgtctcgctg
    11821 cgaccggatg gcggagaacg ccgatactcg cggcattgaa cgcgtgaact atctggcact
    11881 gctggcactc ggcactttct attttctctg cgctttcctg cctatctact tcggcgcgga
    11941 acatgcaaaa accatcattg atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc
    12001 gggcggcatc atgccagcaa tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt
    12061 ctacataccc tacttcatcc tgggctttgt tgccgcaacc tggctcaagt taccggtgct
    12121 ggctattgct gccgccgcgc tggcgatggc gctgatcgat ctgctgcgta aatctcctga
    12181 accgactcaa cctgcggcac agaaagagga atttgaagat ggcatctaat caaaccaccc
    12241 tgccgaacgt cagcgaaaat gaagaaacac tgctgactgg cgtcaatgaa aacgtgtatg
    12301 aagatcggag cattgccgcg gagctgacga aaaaagatat caaccgcgtc gcctggcgtt
    12361 ccatgctgtt acaagcgtct tttaactacg aacgtatgca ggcttctggc tggttgtacg
    12421 gtctgctgcc tgctctgaaa aagatccaca ctaataaacg cgacctggcg cgcgccatga
    12481 aggggcatat gggtttcttc aatacccatc cgtttctggt gacgtttgtt atcggcatta
    12541 tccttgcgat ggagcgttct aagcaggacg ttaacagtat tcagagcacc aaaattgccg
    12601 tcggtgcgcc gcttggcggg attggcgatg cgatgttctg gctaacgcta ctgccgattt
    12661 gtggcgggat aggcgcaagc ctcgcgctac aaggctctat tcttggcgca gtcgtcttta
    12721 ttgtgctgtt caacgtggta cacttggggc tgcgttttgg tctggcgcat tatgcttacc
    12781 gcatgggcgt ggcggcgatt ccccttatta aagcgaatac caaaaaagtc ggccatgctg
    12841 cgtcaatcgt tggaatgacg gtaatcggcg cactggtggc aacctatgtt cgtttaagca
    12901 ccacgctgga aatcacggcg ggcgacgcag tagttaagtt acaggctgat gttatcgaca
    12961 aactgatgcc tgccttctta ccgctggtct acaccctgac catgttctgg ctggtacgcc
    13021 gcggctagag tccgctgcgc ctgattgcgg ttaccgtggt tctagggatc gtcggtaaat
    13081 tctgccattt cctttaaata caatgaggta ttagatgtta agtattattt tgacagggca
    13141 tggcgggttt gccagtggca tggaaaaagc aatgaagcaa atcctcggcg atcaatcgca
    13201 gtttattgcc atcgattttc ccgaaacctc aagcgccgcg ctgcttacat cgcagcttga
    13261 agaggccatc gcccaactgg attgtgaaga cggcattgtt tttctgacgg atttgctggg
    13321 cggcacgccg tttcgcgtag cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat
    13381 taccggcacc aatttgcaac tgctactgga gatggtgctg gagcgcgaag ggttaagcgg
    13441 tgaagaattc cgtgtacagg cgctggagtg tggacaccga gggctgacca gtctggtgga
    13501 cgagttaggc cgctgccatg aagaatgtcc ggtcgaggaa ggaatatgac acacgttctg
    13561 cgcgccagaa ggctgctgac tgaagaggga tggttcgatg accatcagtt gcgtgttgct
    13621 gacggtgtca tcgcagcaat cgaaccgatt ccagtgggcg tgactgaacg cgatgcggaa
    13681 ctgctctgcc ccgcttacat cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg
    13741 gatgacgcgc cggatgtact cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc
    13801 agttggtt
//
LOCUS       DAJTNV010000099        13798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNV010000099 DAJTNV010000000
VERSION     DAJTNV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO199_003180"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO199_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5537128.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO199_003181"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO199_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5537129.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO199_003182"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO199_003182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5537130.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO199_003183"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO199_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5537131.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO199_003184"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO199_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5537132.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO199_003185"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO199_003185"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5537133.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO199_003186"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO199_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5537134.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO199_003187"
     CDS             7490..7639
                     /locus_tag="OO199_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537135.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO199_003188"
     CDS             7840..9423
                     /locus_tag="OO199_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5537136.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO199_003189"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO199_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5537137.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO199_003190"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO199_003190"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5537138.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO199_003191"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO199_003191"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5537139.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..>13798
                     /locus_tag="OO199_003192"
     CDS             13382..>13798
                     /locus_tag="OO199_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5537140.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3494 g         3471 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagtat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgttgggg
//
LOCUS       DAJTNV010000100        13712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNV010000100 DAJTNV010000000
VERSION     DAJTNV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO199_003193"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO199_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5537141.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO199_003194"
     CDS             2220..2768
                     /locus_tag="OO199_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5537142.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO199_003195"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO199_003195"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5537143.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO199_003196"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO199_003196"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5537144.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO199_003197"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO199_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5537145.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO199_003198"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO199_003198"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5537146.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO199_003199"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO199_003199"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5537147.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12381
                     /gene="pepN"
                     /locus_tag="OO199_003200"
     CDS             9766..12381
                     /gene="pepN"
                     /locus_tag="OO199_003200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5537148.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO199_003201"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO199_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5537149.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..>13712)
                     /locus_tag="OO199_003202"
     CDS             complement(13352..>13712)
                     /locus_tag="OO199_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5537150.1"
                     /translation="HGIRNIDRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFA
                     LGLMWLTLIVAETISANSGIGYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLE
                     RLWLRWNPAYHLKEATI"
BASE COUNT         3481 a         3485 c         3464 g         3282 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat gggaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg agctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgctaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca aggatgccgt caatgctgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcgttttac gcttaagatc attaatgaaa taagcccggc ggcgaatacg
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaatcaa atatcccttc ctgctttcca atggtaaccg cgttgcgcaa
    10261 ggcgaactcg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgatacctt taccacgcgt
    10381 tctggtcgcg aagtagcact ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt tggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catagacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg taatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgtc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct ttacgagaag
    12361 ataactaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ctaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actgaccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtatcatcct gaatttcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac cggccagcag gtgcagcagg
    13201 gtacttttac caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcatatcgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc tgtttgcaaa aactctcgcg
    13501 cattcatcgc cagataacca atgccggaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcatcgg
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatctcca
    13681 ccagcccgcg atcgatatta cggatgccat gc
//
LOCUS       DAJTNV010000101        13443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNV010000101 DAJTNV010000000
VERSION     DAJTNV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..801)
                     /locus_tag="OO199_003203"
     CDS             complement(<1..801)
                     /locus_tag="OO199_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5537151.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMP"
     gene            1042..1842
                     /locus_tag="OO199_003204"
     CDS             1042..1842
                     /locus_tag="OO199_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5537152.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1920..2690
                     /locus_tag="OO199_003205"
     CDS             1920..2690
                     /locus_tag="OO199_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5537153.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2738..4096)
                     /gene="mltD"
                     /locus_tag="OO199_003206"
     CDS             complement(2738..4096)
                     /gene="mltD"
                     /locus_tag="OO199_003206"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5537154.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4168..4923)
                     /gene="gloB"
                     /locus_tag="OO199_003207"
     CDS             complement(4168..4923)
                     /gene="gloB"
                     /locus_tag="OO199_003207"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5537155.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4957..5679
                     /locus_tag="OO199_003208"
     CDS             4957..5679
                     /locus_tag="OO199_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5537156.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5676..6143)
                     /gene="rnhA"
                     /locus_tag="OO199_003209"
     CDS             complement(5676..6143)
                     /gene="rnhA"
                     /locus_tag="OO199_003209"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5537157.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6208..6939
                     /gene="dnaQ"
                     /locus_tag="OO199_003210"
     CDS             6208..6939
                     /gene="dnaQ"
                     /locus_tag="OO199_003210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5537158.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7072..7148
                     /locus_tag="OO199_003211"
     tRNA            7072..7148
                     /locus_tag="OO199_003211"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7106..7108,aa:Asp,seq:gtc)
     gene            complement(7279..8325)
                     /locus_tag="OO199_003212"
     CDS             complement(7279..8325)
                     /locus_tag="OO199_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5537159.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8336..9321)
                     /gene="tssG"
                     /locus_tag="OO199_003213"
                     /pseudo
     CDS             complement(8336..9321)
                     /gene="tssG"
                     /locus_tag="OO199_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9318..11201)
                     /gene="tssF"
                     /locus_tag="OO199_003214"
     CDS             complement(9318..11201)
                     /gene="tssF"
                     /locus_tag="OO199_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5537160.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11217..11711)
                     /gene="tssE"
                     /locus_tag="OO199_003215"
     CDS             complement(11217..11711)
                     /gene="tssE"
                     /locus_tag="OO199_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5537161.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11708..12538)
                     /locus_tag="OO199_003216"
                     /pseudo
     CDS             complement(11708..12538)
                     /locus_tag="OO199_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12525..13439)
                     /gene="tagK"
                     /locus_tag="OO199_003217"
                     /pseudo
     CDS             complement(12525..13439)
                     /gene="tagK"
                     /locus_tag="OO199_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3077 a         3501 c         3587 g         3278 t
ORIGIN      
        1 tggcatttcc actggcaaca ctttatctgc cattaactcc accaattctc cgcgagcgat
       61 ttctttgtcg atcatgtagt cggacaaaca cgcaatcccg ttgccactca ggcaaagctg
      121 tttcagtgtt tccccactat tggatgacaa accgtacttc acctcatgta attgtccatc
      181 actacaggct atcggccagg tattgaggga agcgggttca gtgaatccca ggcaaacatg
      241 ttgctttaaa tcgtcgatcg tttctggctt cccgtagcgg gaaatataat cgggggaggc
      301 gataattttt cgataactgt taaataacgg cctggcacgt aagctggagt ccgttaacgt
      361 accagcgcgt atcgcgacat ccacttttct ttcgatcaaa ttaataatcg tttcggagga
      421 gactagcgat aaagtgactt ccggatagcg ttcacggaaa ggcttaatta acggcatcag
      481 aaagtgcagc accactggag ttgcggcatc gatccgtaac agtccacgcg gtgtattacg
      541 cgtctccata atttctgatt ctgccgctgc catctcctgc aaaattgact gtacgcgacg
      601 aaaataacgc tcgccttctt ccgtcaggct aagttgccgc gtggtccgat taagcaggct
      661 aacgccaagt ttcatttcca gctttttcac cgcccggctt accgctgagt ttgcttgccc
      721 taattgttcc gctgcccggc taaagctgcc gctttcgacg accgaaacaa aaatggcgag
      781 ttcttccgac gtggctttca tttttgctcc tgttgcaaaa tagatgagat attttgaatt
      841 tatttgtcat taaaccatca ggatgcgtga tatgtcatcc gatttaatgt tctcaataat
      901 gagcaaattt ctgacaggtg taagcacttg cttacataac aatatacaat tgctcgttga
      961 aagagtgagc taaaatccct ataacagtag aaccctcccg agtgcggaag ggttgacgta
     1021 atagaggttt caaagtcaaa agtgcgaaaa aacacctatg ccatgcgcta tgttgccgga
     1081 caacctgcgg aaaggatctt accgccgggg tcttttgcga gcatcggcca ggcattacca
     1141 cctggagaac cgttaagtac cgaagagcgt attcggatcc tggtgtggaa catatacaaa
     1201 cagcaacgcg ctgaatggtt gtcggtatta aagaactacg gcaaagatgc acatctggtg
     1261 ttattgcagg aagcgcagac aacgccagag ttagtacagt ttgcgaccgc taactatctt
     1321 gccgccgatc aggtgcccgc tttcgtgctg ccacaacatc cttccggtgt aatgaccctt
     1381 tcggcggcgc atccagtgta ttgctgcccg ttacgtgaac gagaacccat tttgcgtctg
     1441 gcgaagtcgg cactggtgac ggtctatcca ttacctgaca cccgcctgtt gatggtggtt
     1501 aatatacacg ccgtcaactt cagtctgggc gtggatgtct atagtaagca gttacttcct
     1561 attggtgatc agatagctca ccacagcggc ccggtcatta tggcgggaga tttcaatgcc
     1621 tggagccgta gaaggatgaa cgcgttatat cgctttgcgc gggaaatgtc gctgcgccag
     1681 gtgcgtttta ccgatgatca gcgccgtcgg gcgtttggcc gcccgctcga ttttgttttc
     1741 taccgtggtc tgaacgtcag cgaagcttct gtattggtta cgcgcgcttc cgatcacaat
     1801 ccgctactcg ttgaattcag tcccggcaag cctgataaat aaggtatgtc aggtctgcca
     1861 cagggcagac caacgtttgg cgctgtcctt ttatactcaa acaacaaaag gacagcgcaa
     1921 tgacaacaca atcccaccat gaccacgtag aaaagcagtt tagctctcaa gcctgcgaat
     1981 atctgaccag taccgtacat gcatccggac gagatttaca gcgcctggcg gtgcgtctgg
     2041 ctgattatcc tggtgcaagt gcacttgata tgggctgcgg agcagggcat gccagctttg
     2101 ttgctgcgca aaacgtgagc gcggtggtgg cgtatgactt atctgcccaa atgctggatg
     2161 tcgtggcaca agctgccgaa gcccggcaac tgaaaaatat caccacccgc cagggatatg
     2221 ccgaaagtct gccatttgcc gataacgcat ttgatattgt tatcagccgt tattctgccc
     2281 atcactggca tgatgttggt gcagcactgc gtgaagtgaa ccggatattg aaacctggcg
     2341 gtaggatgat tgtgatggac gtaatgtctc cgggtcatcc agtgcgcgac atctggttac
     2401 agacggtaga agcattacgc gatacctctc acgtacgaaa ctacgccagc ggtgagtggt
     2461 tgaggttaat caatgaagcc aatctgatag ttgataattt aattacagat aagttaccgc
     2521 tggaattttg ttcatgggtc gcgagaatgc gtacgccaga agcgttagta gacgctattc
     2581 gcatttacca acagagcgca tcgacagagg tgagaacgta ttttgccttg cagaatgacg
     2641 gctctttcac cagtgatatc atcatggtag aagcacataa agcggcataa ataaaaaagg
     2701 caccggggga atcggtacct ttttattatc tggtttgtca ggaatctggc atgttgttgt
     2761 ttttcacaaa caacgtcagc ttgtcgcctg gttgcagatt cgcagtgtcg ctgttccagc
     2821 gcatcacatc tttgatgttc acgccgtggc gtttagcaat gcttgaaagc gaatcgcctt
     2881 tgcgcacacg atacgtaatg ctatcgctgt tgtttgccag tcgctgtgcg ctactgcctg
     2941 caccaatcgt caaactttgg cctggcttca gcttagatcc gcgcagtttg ttccactgct
     3001 gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct tgaaagcgtg tcgccagagc
     3061 gtacggtgta aacacggctg ttaagcggcg tattgttggc aaccagcgtc gactgtacag
     3121 cagcaatttc gcctgaagcg agagattcac gcagttgatc tgcatgcttc tttggcacca
     3181 tcacgtactg cggcccactt gcgcccagcg tggagccttt cacgccagcg ttgaatgtct
     3241 tcagcttgct gacggaaatc cccgccatat ctgcaacctt cgccatttca accgggctac
     3301 tcaggtgcac acgcgccaga gcacggcttt catcggtcgt tggcagacgt acgccataac
     3361 gcttgctgtt tttgagaata tcactcaatg ccagcatttt aggcacgtac tgcttcgttt
     3421 cctgcggcaa cggtaacgac cagaagtccg tggatttccc acgcgctttg ttcgttttaa
     3481 ttgccttcat gacccgacct tcgccgctgt tataagccgc tacggtcaga agccagtcgt
     3541 cgtcaaacat tttgttcaga cgctgcatca tattcagcgc ggcagttgtt gaagcaacaa
     3601 catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc ataattgcgc cccgtgctcg
     3661 gaatgatctg ccagatgcct gcggcattgg cgccagacgt tgcgtgagga tcaaaagcgc
     3721 tctccactat gggtagtagt accagttcca taggcatgtt acgtttttta acttgccctg
     3781 ctatccagta catatacggc tctgcccgta aagttacatc gtggagatag ctcttattgc
     3841 gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc cggaattccc atctttagct
     3901 cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat agacgtccca tcgtccatcc
     3961 atcgtgcctg acttgtaaac tttgctgctt ccccttgacc agctgcagaa aggctctgtg
     4021 cgtgctgttg aacgttgccg gtactctggc aacccacgag caggacagag gcgagtaata
     4081 tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa gacgaccgat cataacggcg
     4141 aacggagccg atgacaagaa agttttatca gaacctatct ttctttgacc ttaaccatgc
     4201 aaaacgctct tcaggttgtt gcaataatgt ttcttcatta attccattaa ttaaatcaat
     4261 atcttccgtt cttaaaaaaa cattaatttg ccgctcattt ttcagaatta cgggtagtgt
     4321 tatttgattt tttgcccgta actccttaac tttacgataa taatcattta tggacaaatc
     4381 gtgcggaaaa atactcaaag caaacttcat atttgataac gtatattcat gagcacaaca
     4441 taccaatgta tcgtcaggta acgcacttag ctttttaagt gattgataca tttgtaaagc
     4501 tgtcccttca aacaaccgac cacacccacc agaaaacagc gtgtcgccgc aaaatagata
     4561 aggtttactg aagtaacaga tatgtcctaa agtgtgaccc ggtgtggtaa ttacactaaa
     4621 ttcatgccct aaaacgaagg cagtttcgcc atctttgact acctgtgttg ttcccttatc
     4681 ttgtgtctct tgtggaccat acaccacaat ttgcggaaac ttttccacca gttcttttac
     4741 gccgccaacg tgatcgtggt gatggtgggt tagaaatatg gcctccggtt gccagttatt
     4801 ggcggtaatg gcgtttaata ctggctctgc gtctccggga tcgacaatca ggcagcgacc
     4861 tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag gcgggaatac tgttaagatt
     4921 catagattac ctctcagtgt aaaacggaag gttgtgatga aaccggcaag agtccctcaa
     4981 actgtcgtgg ctcctgattg ctggggcgat ttgccctggg gagagcttta tcgcaaggcg
     5041 ctggagcgcc agctcaaccc gtggttcact aaaatgtatg gttttcatct gcttaagatt
     5101 ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg tttctcatca agtgaatgtt
     5161 tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac ttcatcttcc ttttgccgat
     5221 aaatccgttg atatttgtct actggcacat accttgccgt ggtgcaccga tccgcatcgt
     5281 ttattacgtg aagccgatcg ggtactgatt gatgatggct ggctggtgat tagtggcttc
     5341 aaccccatca gtttaatggg attacgcaaa cttgtgccgg tattgcgcaa aacctcgccc
     5401 tataatagcc ggatgtttac tctgatgcgg caactggact ggctctcttt gttgaatttt
     5461 gaagtgctgc acgccagccg tttccacgtt ctcccgtgga acaaacacgg aggaaaacta
     5521 ttgaatgcgc atattcctgc gcttggttgc ttacaactta ttgttgcccg gaaacggact
     5581 attcctttaa cgctaaatcc gatgaaacag agtaaaaaca agccacgaat tcgccaggcg
     5641 gttggagcca cccggcaatg tcgtaaacca caggcttaaa cttcgacttg gtagcccgta
     5701 tcttccagtg tgggattcat cgccgcggca cgagccagtt catcacagcg ttcgttttcc
     5761 gggtgtccgg catggccttt aacccattcc catttgattt gatgctgccc caatgcagca
     5821 tcaagacgtt gccagagatc gacatttttt actggttttt tgtctgctgt tttccagcca
     5881 cgttttttcc agttatggat ccactgggtg ataccctggc ggacatactg gctgtcggta
     5941 ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg cgacaatagc ggccatcaac
     6001 tccatacggt tgttggtggt gcgggtgtag cctgcgctaa aggttttctc gcgtccgcga
     6061 tagcgtaaaa tagcgccgta acccccaggt ccaggattac ccagacacga accatcggtg
     6121 aaaatttcta cctgtttaag catctctggt agacttcctg taattgaatc gaactgtaaa
     6181 acgacaagtc tgacataaat gaccgctatg agcactgcaa ttacacgcca gatcgttctc
     6241 gataccgaaa ccaccggtat gaaccagatt ggtgcacact atgaaggcca caagatcatt
     6301 gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg gcaataactt ccatgtttat
     6361 ctcaaacccg atcggctggt ggatccggaa gcctttggcg tacatggtat tgctgatgaa
     6421 tttttgctcg ataagcccac gtttgccgaa gtagccgatg agtttatgga ctatatccgc
     6481 ggcgcggagt tggtgatcca taacgcagtg ttcgatatag gctttatgga ctacgagttt
     6541 tcgttgctta agcgcgatat tccgaagacc aataccttct gtaaggtcac cgatagcctt
     6601 gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc tcgatgcgct atgtgcccgc
     6661 tacgaaatag ataacagtaa acgaacgctg cacggggcat tactcgatgc ccagatcctt
     6721 gcggaagttt atctggcgat gaccggtggt caaacgtcga tggcttttgc gatggaagga
     6781 gagacacagc agcaacaagg tgaagcaaca attcagcgca ttgtacgtca ggcaagtaag
     6841 ttacgcgttg tttttgcgac agatgaagag ctggcagcac atgaagcccg tctcgatctg
     6901 gtgcagaaga aaggcggaag ctgcctctgg cgggcataaa tacatgtaaa aggcgctaaa
     6961 aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa gatgagaaaa atcgttgacg
     7021 aaggtcgagg caatccgtaa tattcgcctc gttcccaacg gaacacaacg cggagcggta
     7081 gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc gcgggttcga gtcccgtccg
     7141 ttccgccaat attcagaaag cccgaatcag taatgattcg ggctttcgtc gttttggtga
     7201 agtgagaatt tttcactccg aagtaaaacg gatatgattt aaaaaatggc cagacattat
     7261 aaattctggc cacttctgtt actccagcgc atcccatttc tccagcagtt ctactggaat
     7321 ttcatttatt acctccgaga cgcgttttcc gaccaggcgt tctgcctggc gcagcaaagg
     7381 aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga cggatgacac gcaggcgttc
     7441 cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg ccggtcatcg gtgatatttc
     7501 ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc atctgttcag tatgaacgat
     7561 ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg atctccggcg tggtgctctg
     7621 cactgctccc ggtaacaggg ccagcagttg cgtcagacgg gagaagtccg gtgcctggtc
     7681 attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg gtctctgctg cctggcgaaa
     7741 ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc agttgctggc gtaccgattc
     7801 tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa cgctccacat cccgcacctg
     7861 aaggcgcatg gcagcattat ttgacagtgt aatgccacga atatccgcca ttacaccttc
     7921 gtggtccagc aacgccgcca gcgcgttact gcgtgccacc gcagcatctt cggcggtgat
     7981 atcttcagtc gcgagcagtt gaggatggat ggcgtccggg tacgtggagc acaacgtttc
     8041 cagcagcgtg agcgcctccg acagtcccct ggcaccggcc tgctggatac ggcagcgcag
     8101 caggacaacc agaatgcgga tatctttgct gcgcatcagc agacgatggg cgtcccgctc
     8161 aagctccgcc cagttaatgt tttccggcgt actgacaaaa tcgccatatt gcgcttcttc
     8221 acgcggtgcg gcgcgggtga acagcagcag gtattccgga tcgtactcca gatccgggcc
     8281 gcaggggcat tccgccgaaa ttgggtcaga caggaatatt tccatttaat tatcctcagt
     8341 gcgccaggta ctgttctggt tcgaaaatca tgcccgtcac cgggttgtcg tgcatggctt
     8401 tacccgccca tgcagtccaa cccagttggt acggaccacc ggtaacagcc ggaggggcgg
     8461 cgtggggctt tagctgtagc tcaatttccc agcagtattc ataaccacta aaggcgcgga
     8521 ccagttctgt caggacgggc aggttattgc tgccaggcag aaaacgtcgg taatcctcca
     8581 gcgtcagcgg gccgataacc agccggaact tatactgcat gtccggcacg gcctggccga
     8641 tgagtgcacc attgccaata acggaagagg gggcaggcat ccccagccgg gtgatctcat
     8701 cgtccgccac ggctatccag tgcaggacat actcctgtat ccggaaaggt acaccgaaat
     8761 agtgcgccag cgtggtggcc agaccgtccg gattgcggga ctcacgcacc agatgggccg
     8821 atgccgccag acgagcgtga tctggcaacg ggctgtcagc gctctcccgc aaatcctgcc
     8881 cgctgaggct ggcgacataa aaggcaaagc ggtcatgttg cggtttatcc agaccgcccc
     8941 cagcggactg ggcgctgcgc catgcctggt aaaactgcgt cagccagcgg tgatgaaaaa
     9001 gatcagaaaa acgggtcagc gtcggctttc agtccggtta cgcgccagtt cggtgtagtg
     9061 cagcggcagt ggaccgttcg gcccccacag cccgaggctg tacagactga tgtgcaggcg
     9121 cccttcctgc cagcccacac tggcgatttc tcgtggcgca aaggtcagcg cgggcgtttg
     9181 tccgaggcga aatttttcca tccgtggttg ccaggtgttg cccgccggaa cggtacacaa
     9241 ctgcacatcc acgcggcgca tcaggttcag aaaaccgtaa cgccaggggg gggcctgcgc
     9301 ctgagtgaaa tcgttgctca tacgcttccc cgctgtcccg ccctgacagg ccaggtcata
     9361 attttgccgc gctgcatgct gtgcagcgtc atctgcgaga aggtattaat ggaaacatgg
     9421 cgggcgatat agtgttccag caccaggccg aacagatacg gactgatgcc ggaaaaacct
     9481 tcttcatcca ccgtcagttc acaactgacg ccacgcccat agaccagtag tccggaaccg
     9541 ggcagacggc gggtgaccgg ggtcgtttta caaccaatca ggctgcgcac ctggcgcgat
     9601 tgcggactgt catgagcagg aataaacagg ttcagcagat ctcgcaacgc ctggccgccg
     9661 gtacggtgat ccagatcggc cagcggcaga taattaaaag ataactggcg gatcaaccgc
     9721 catgccatct cacgttcagc cagcgggggc tgcggcgagc gtggcgggcg gataagccct
     9781 acgcccgcca ccgggatggc tgcatccacg gttagatcgt cccggccatt gcgggcaata
     9841 agacagggca gatcgcggtt agtgaccatg gccgtgatgg tgatgtggcg cagattttcc
     9901 gggtacggcg cttcatactg gtcaaccagc gacaggaaca cttccgagcc cgtatagggg
     9961 gtacgggtgc cgtaacggcg ggcgttttct gataagcggc ggggctcccg gcgcagggaa
    10021 aaataacgtc cgtgattgcc ttcatcatta ttgcgggtgt ggtagagcgg gcgaaaggcc
    10081 atcttccgtg tggtgtcggt ttccagccct tccacttcct ggaccgagaa cacctcataa
    10141 tccagcggat gggttctgtc caccaccaga tgctgttccg tcgtgctgtg cgtgacatcg
    10201 atccgggcgg tgacgcgggg aaacaggttg ataaccgggg tacagaacag gctgaactgt
    10261 gccgcatttg tctggtggat cagccagtcc ggcggcaggc ggttcagcag gataacaatc
    10321 tccgccacgc caccgtcaat tttctgtaat ccggcagaca ggcctgtcgg ggtgaaaaaa
    10381 tagaagcgtt ccggacaggc aaaatattca tgcagcaggt tatgaccgtg aaacacgttc
    10441 cacgccagcg gcagcagccc ctgatccggc tccaggcctt cgtacataac gggctgttgc
    10501 agattcacat ccagcgcgcc gtcaaaatgc cccggaatgc cagccagggt cgcgatggcg
    10561 ctggtatgta gaagttctaa caggtgtgag gccgtacgct cctcgccgca gagataaaac
    10621 ggcagcctgt ccagtccggc aagctgactg aacgttagtt caccaaaggt gcgcagggta
    10681 atacgcagcg ccccggcgac gtgaatgttg gccggcaggt agcggtgcag ggcaggcata
    10741 tccggtggcg cggcggtcag tcgcgcctct tcgatggcga gcggccacag cgttacatcc
    10801 tggctgctgc gaaactggca ggcagtgctt tcgccttccg gaatggcgga gaaaaaggct
    10861 gtgtcgcgcg ggacggtaaa tcctttcgcc agatcgcctt cttccgtatc gggatgcaac
    10921 tgcgccacgc tcattgaggg cgtgggtgtg acgtaattgg ggctgaccac ttccagcagg
    10981 cgctgggtga aacgggggaa ttcagcgtca atttttagct gcgtgcgggc ggtcaggaag
    11041 ctgaaggcct cgaccattcg ctctacatac ggatcggcaa tatccgttcc ctgcatgccc
    11101 agccgggcgg cgactttggg atgacgggcg gcaaattcgg ctcccgtttc ccgcagatag
    11161 ctcagttcgc ggttgtagta ttccagtagc cgtggatcca tcggtctctc tctgaattaa
    11221 aaaaacgtaa tgcgactctg ctccatatcc agcgcgctac gtacccggaa tgcagtcgga
    11281 tacggctgcg tgaggatctc gccgcgaatt tcaaactgta atgtgttatg gccactctgg
    11341 cggcgctcat cctgaagtgg gatgatttgc agagtggacg ctttcagccg gggttcaaac
    11401 cgggtgatgg ttcgcaaaat ggccctgcgg atatcgtccc acttgtgctc atacagaaag
    11461 ctgcccgcca gtggcggcag gccgtagtta agtacggacg ctgccgcgta cggataacgt
    11521 gcagcatcaa tatcatcttc atggctgacg gtgttgagca gaaatgacaa atcccggcgg
    11581 atgatctcct taatctgaac cggactgacg ctgataccgt ggtcgcgttt ctggtttggg
    11641 gcgtcgtcac acaggcggtc aaacagcgtg ggcagcaggt gattgcctgg acgctggcgg
    11701 gaagcgctca cgcgtcgctt ccttcagcgg tgttaaaggt acaggtggac aaatccagca
    11761 ggctgatatc gccgtgaccg gtgagccaca ccctctgacc cagcgcctgt accagtgtct
    11821 ctcccggacc gtcctgccag accgtttccc ggcacagacg cagggagtcg gcagcctttt
    11881 cagagccgct gtagcgggga aacagctagg cactgtgtgt atcgccattg ttcagggtga
    11941 cgttgacggg tttccagatc aaatcgatca gatgcgcagg ccgcggcgat ttcagcgaac
    12001 agatttgtga aaacggcagc cagatatatg ctccgcccgc gatcagttcg agtaccggac
    12061 cggtacggga gtcgctgtcg ctaatccagt caaatgcgcc gccattccac tgcccggaag
    12121 tgtctgttat ggcctcaagc gcgcggctgc ggtgagcatc agcttcctgc gcttcaccac
    12181 gggcgttaca ggcgagggcg gtcagcagat cctccatcca ggcgggcggc gggagaataa
    12241 caccgggccg ctgttcgccc tgaaagcagg cgtggcggta tatctcacag cggatcagct
    12301 cgccaaatac ctgcgcttcg cgagtataat ttgcgtccat ccgggcgcat agctggatct
    12361 gttgcagtgc ccgcgaccag tcgcctgcca cgcacaataa ctgaaacagg ctgtggcggc
    12421 gggaggcctg cgctgggtgc atcctgacct gttgctctgc catctgaatg ccctccacca
    12481 cggaataatc ccgcagcagc gccgacaagg taatcggtag ctcgttattg tttgtcatga
    12541 tatatctctc catgttgtgc cgggtttatc cggtaatggc tgtcaatgcc aatgatgcga
    12601 tgctctttcc gtgtcagatc tggcagtacg gtgttatgcg ctgtttccgg ggcgtattcc
    12661 ggggaaagta aatgcaggat atcaggggga gtcttttcga caggccagat agtttcatct
    12721 tcgccagtgg catccagtac attgagaata gcgtcaatgc ttaacccacc agtgaccata
    12781 tcctgcaatg tctcggcttc ggtgaccagg tcacgcaatg aatccagatc ctgcgaggag
    12841 ggctcttcgt taaaaggaaa aggatcagca tggcgattaa gatcttgttc gggagataac
    12901 aacgcctcgc ggtattcctg ataaagtcgg gtcagggcat ttcctttttc cgcaccttct
    12961 ccggtggagg aggctccatg aacaggaatg atatcgaatg gattttgtga tgtcagatgc
    13021 tgctgcctga accaatccag atccagatat tcggttattg cttccgacgt gatggcagtc
    13081 tgcgatatcg tttttactgt gctcaggttc ggagatgatg gattgccatg tacaaggcac
    13141 tatgaagata acccccattc tatcaggtcg ccttcattca gacgcatacg gtgatgaggt
    13201 tccacaatct ggtcgtttac cgtacagcaa tattcggtag actgattgat aatccaccat
    13261 gcttcttcgt ggcgggtcag agtgagggca atctttacat catcattcac cttaaaaaca
    13321 ggaatccagg gagaggtaac ggtgaagata atttgtgttc cttcagtcgc gtaaattgtg
    13381 ttatcggcga tcccgtgcgc ctgtattttt tggagtcccc acgtaatatt tggtttcatt
    13441 gtg
//
LOCUS       DAJTNV010000102        13124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNV010000102 DAJTNV010000000
VERSION     DAJTNV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(109..429)
                     /locus_tag="OO199_003218"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="OO199_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="OO199_003219"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="OO199_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="OO199_003220"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="OO199_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5537162.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="OO199_003221"
                     /pseudo
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="OO199_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="OO199_003222"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="OO199_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5537163.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="OO199_003223"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="OO199_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5537164.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="OO199_003224"
                     /pseudo
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="OO199_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="OO199_003225"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="OO199_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="OO199_003226"
     CDS             10723..11613
                     /locus_tag="OO199_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5537165.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="OO199_003227"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="OO199_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5537166.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12603..12679)
                     /locus_tag="OO199_003228"
     tRNA            complement(12603..12679)
                     /locus_tag="OO199_003228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13124
                     /locus_tag="OO199_003229"
     CDS             12922..>13124
                     /locus_tag="OO199_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5537167.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3217 a         3402 c         3331 g         3174 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctactgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgag
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcct
//
LOCUS       DAJTNV010000103        12874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNV010000103 DAJTNV010000000
VERSION     DAJTNV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..627
                     /gene="csgD"
                     /locus_tag="OO199_003230"
     CDS             <1..627
                     /gene="csgD"
                     /locus_tag="OO199_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5537168.1"
                     /translation="HGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQRSLDDISS
                     GSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWPHINGVFYA
                     MEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHREKEILNKLR
                     IGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            632..1021
                     /gene="csgE"
                     /locus_tag="OO199_003231"
     CDS             632..1021
                     /gene="csgE"
                     /locus_tag="OO199_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5537169.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1046..1462
                     /gene="csgF"
                     /locus_tag="OO199_003232"
     CDS             1046..1462
                     /gene="csgF"
                     /locus_tag="OO199_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5537170.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1489..2195
                     /locus_tag="OO199_003233"
                     /pseudo
     CDS             1489..2195
                     /locus_tag="OO199_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2328..2819)
                     /locus_tag="OO199_003234"
     CDS             complement(2328..2819)
                     /locus_tag="OO199_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5537171.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2921..3475)
                     /gene="ycdY"
                     /locus_tag="OO199_003235"
     CDS             complement(2921..3475)
                     /gene="ycdY"
                     /locus_tag="OO199_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5537172.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3499..4236)
                     /gene="ycdX"
                     /locus_tag="OO199_003236"
     CDS             complement(3499..4236)
                     /gene="ycdX"
                     /locus_tag="OO199_003236"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5537173.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4291..5229)
                     /gene="ghrA"
                     /locus_tag="OO199_003237"
                     /pseudo
     CDS             complement(4291..5229)
                     /gene="ghrA"
                     /locus_tag="OO199_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5464..5551
                     /locus_tag="OO199_003238"
     tRNA            5464..5551
                     /locus_tag="OO199_003238"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5498..5500,aa:Ser,seq:gga)
     gene            complement(5701..5982)
                     /locus_tag="OO199_003239"
                     /pseudo
     CDS             complement(5701..5982)
                     /locus_tag="OO199_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6264..7268)
                     /locus_tag="OO199_003240"
     CDS             complement(6264..7268)
                     /locus_tag="OO199_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5537174.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7340..7900)
                     /locus_tag="OO199_003241"
                     /pseudo
     CDS             complement(7340..7900)
                     /locus_tag="OO199_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7985..8182)
                     /locus_tag="OO199_003242"
     CDS             complement(7985..8182)
                     /locus_tag="OO199_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537175.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8194..8685)
                     /locus_tag="OO199_003243"
     CDS             complement(8194..8685)
                     /locus_tag="OO199_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537176.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8682..9056)
                     /locus_tag="OO199_003244"
     CDS             complement(8682..9056)
                     /locus_tag="OO199_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5537177.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9103..9477)
                     /locus_tag="OO199_003245"
     CDS             complement(9103..9477)
                     /locus_tag="OO199_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5537178.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9640..9861)
                     /locus_tag="OO199_003246"
     CDS             complement(9640..9861)
                     /locus_tag="OO199_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5537179.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(9924..10400)
                     /locus_tag="OO199_003247"
     CDS             complement(9924..10400)
                     /locus_tag="OO199_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5537180.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10416..10889)
                     /locus_tag="OO199_003248"
     CDS             complement(10416..10889)
                     /locus_tag="OO199_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5537181.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11231..12052)
                     /locus_tag="OO199_003249"
     CDS             complement(11231..12052)
                     /locus_tag="OO199_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5537182.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12152..12325)
                     /locus_tag="OO199_003250"
     CDS             complement(12152..12325)
                     /locus_tag="OO199_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5537183.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(12445..>12874)
                     /locus_tag="OO199_003251"
     CDS             complement(12445..>12874)
                     /locus_tag="OO199_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537184.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3245 a         3201 c         3177 g         3251 t
ORIGIN      
        1 catggtcata cattattgtt gatcactaaa ccttctttgc aggcgacagc tctcttgcag
       61 caccttaaac aatcgctagc aattacagga aaattacata atattcaacg ttctctggac
      121 gatatctctt caggctctat tattcttctg gatatgatgg aagcggataa aaagcttatc
      181 cattattggc aggatacttt gagcaggaaa aacaacaata tcaaaatatt gttgctaaat
      241 acgcctgaag attacccgta ccgcgacatt gaaaactggc ctcatatcaa cggcgttttt
      301 tatgccatgg aggatcaaga acgtgttgtc aatgggttgc aaggcgtcct gcgtggcgaa
      361 tgctacttta cgcaaaagct tgccagttac ctgattacac attctggtaa ctatcgttat
      421 aacagcacgg aatcagccct ccttactcat cgggaaaaag agatcctgaa taagctgcgt
      481 atcggcgcgt ctaataacga gatcgctcgt tcgttgttca tcagcgaaaa tacggttaaa
      541 acgcatcttt ataatctttt caagaagata gccgtaaaaa accggacaca agcggtttcc
      601 tgggcaaacg ataacctcag gcgataaagc catgaaacgt tatttacgct ggattgtggc
      661 ggcagaattt ctgttcgccg cagggaatct tcacgccgtt gaggtagaag tcccgggatt
      721 gttaactgac catactgttt catctattgg ccatgatttt taccgagcct ttagtgataa
      781 atgggaaagt gactatacgg gtaacttaac gattaatgaa aggcccagtg cacgatgggg
      841 aagctggatc acaataacgg tcaatcagga tgttattttc cagacttttt tattcccgtt
      901 gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt caaactgaag aagcactaaa
      961 tcgtcgccag ataaatcagg cgttattaag tacggacgat ttggcgcatg atgaattcta
     1021 aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat gcagtagttc tactcatgct
     1081 tatttcgcca ttaagttggg ctggaaccat gactttccag ttccgtaatc caaactttgg
     1141 tggtaaccca aataatggcg cttttttatt aaatagcgct caggcccaaa actcttataa
     1201 agatccgagc tataacgatg actttggtat tgaaacaccc tcagcgttag ataactttac
     1261 tcaggccatc cagtcacaaa ttttaggtgg gctactgtcg aatattaata ccggtaaacc
     1321 gggccgcatg gtgaccaacg attatattgt tgatattgct aaccgcgatg gtcaattgca
     1381 gttgaacgtg acagatcgta aaaccggaca aacctcgacc atccaggttt cgggtttaca
     1441 aaataactca accgattttt aagccccagc ttcataagga aaataatcat gcagcgctta
     1501 tttcttttgg ttgccgtcat gttactgagc ggatgcttaa ccgccccgcc taaagaagcc
     1561 gccagaccga cattaatgcc tcgtgctcag agctacaaag atttgaccca tctgccagcg
     1621 ccgacgggta agatctttgt ttcggtatac aacattcagg acgaaaccgg gcaatttaaa
     1681 ccctacccgg caagtaactt ctccactgct gttccgcaaa gcgccagggc ttacaaaacc
     1741 tgcttaacga gcgcaaggtt attcgtgcgg cacaagaaaa cggcacggtt gccattaata
     1801 accgaatccc gctgcaatct ttaacggcgg caaatattat ggttgaaggt tcgattatcg
     1861 gttatgaaag caacgttaaa tcgggcggtg ttggggcaag atattttggg atcggtgcag
     1921 acacgcaata ccagctcgat cagattgccg tgaacctgcg tgtcgtcaat gtgagtaccg
     1981 gtgagatcct ctcctcggtg aacaccagta agacgatact ttcctatgaa gttcaggccg
     2041 gggttttccg ctttattgac taccagcgct tgctggaagg ggaagtgggt tacacctcaa
     2101 acgaacccgt tatgctgtgt ctgatgtcgg ctatcgaaac aggggtcatt ttcctgatta
     2161 atgatggtat cgaccgtggt ctgtgggatt tgtaaaataa agcagaacgg cagaatgaca
     2221 ttctggtgaa ataccgccat atgtcggttc caccggaatc ctgacagata aaaatgccgg
     2281 aattaataat ccggcattta ctgtttaagc gaaagcgtga gtgcccctca cgctttattt
     2341 ttgatttcct gctcacggtg agccgtcttt gcactacgcg ccgccagcca caggccgctg
     2401 tttttcattg cgtaaccaaa taccagccca agcgccagcg aaggtaacac cagtttccag
     2461 tcaccctgcc cggcaaacgt cgcgcatgcg cctataaacg tccccggaac aaatgaaagc
     2521 agcagctgtt tggcctgaat acacatcaga aacgcgacaa taccggttat gacataaccg
     2581 agaatttcca gatgtggtgc cagtgcacta ccgtaaataa tgaccatcgc ccacaccacg
     2641 ccactaagca gggttgcagc ggagatcgcc agccctttca gcccaccttg cgggcaagca
     2701 aagtaggccg tacagcccag gaagcccgcc cagcttagta agcccaggga aacagccacc
     2761 catccccaga taccggagag aatgcctgtt gtgattgcaa tagagagaag tatgttcatg
     2821 ccgcgcatga tagcagaaaa cgcggccatg aatgagatcg agcacacatt ttatgcaaca
     2881 acaaattatc tgttgcagtt atgagtgacg atgatcacat ttactcttca gaatcttctt
     2941 ccagctcgtc ccacattgca ctaatggcat cacgggttag cggtgccatg gtgcgccaga
     3001 aaggcgtggt tgcatgggcc tccactttgc cgaggaacgc accacaccag ggtaacagat
     3061 actcactgaa cagtgtttcc agtgcttcgc tctcatcttc cgttgactga tcttccagcc
     3121 aggaagccgc aagcagcaat gtgccgatgt gatcggctgg cgtatccgcc agtggcatcc
     3181 ctcgttcgga aagaaaagcg cgcacttccg cttccgttgc gtcctcaacc catgcgctac
     3241 gatatggcgg cacggcacat tcatcgccga taaacaacgc attgtaatcg gcagagactt
     3301 gcgtcatatc acagcttttc tgtaaacgcg tcagtaactc atcctgctcc agtggccagt
     3361 tcgcagccag tttcccctca cgaatcagag taaacagcgg caccagtaaa ggatcttgcg
     3421 gttggcggta atagagcgaa cccagcacac gacagaggat agaaaactcg ttcattaata
     3481 tatttccatt actagtaatt aaaggtctgc aaattccgca atcggtgcca taccgcgaga
     3541 ttcaaggaag ttcagtaagc ggcgcggaga aacattcaaa atgcgctcta gcggaaaatc
     3601 taccgcgtcg aggattttaa gacactcttc aaattccccc atggtaaacg cggtgtgaga
     3661 atccgagcct aatgccaccc aacctccagc atcacgtacc gctgcagcta ccgcacgaca
     3721 gttgtcttca ctgcccttac gtgagtgtaa aaatgaggaa ttattgattt ccagcgccac
     3781 ctgatgtttc gcggctgctt cagcaacggc tttaacatct atttcatatt tcggatttcc
     3841 gggatggctt attatatgca cattgccgct ggcgatagtg gagatcatcg cttgtgtatt
     3901 ggtcgctttg tcatgtggcg caaaaaccgg ctcatgaaaa ccggcaataa tcagatccag
     3961 cgagtcaaac attttaccgc tgcagtcaat ttcaccatca acatttttaa tgttagcttc
     4021 aatgccgcgc aggatcccta ccccatcaac cactcgcggc cagatacgca tgttaatgaa
     4081 gtgccagtga tgcggcgcat cttccatatc cgggccatga tcggtgatcg caaaaagttt
     4141 aatgcccttt tgtttggcct gggcaatgta atcactcaat gtgctatatg catgtgtgct
     4201 ggcaacggta tgcatatgaa ggtcgacggg atacataact ctctcctgta ttcaattccc
     4261 gccaaggata gcaggaatcc tgatgcttta ttagtagcca cgtgcgcggt cgacttgccc
     4321 gcagaccctc tccccttttt cgagctgggc aatagtgcga gaaatgtact ccacagcttc
     4381 agcgggacgg gtaatcgcgg cgacatgtgg tgttatcgtc acgcgtggat gttgccagag
     4441 cggactttca ggcggtaagg gttcacgatt aaaaacatcc agcattgcgc ctttaacttt
     4501 cccgctatcc agcgccgcga gcaggtcatc ttccacaaca tgaacaccac gcgccaggtt
     4561 gaggagatac gcgccatcag gtaatttttc gagtaattgt tgattaataa tgccgacggt
     4621 ttcaggggta ttcggtaaca aattaatcaa tacccgacat tggctcagaa atgcagacag
     4681 ttcttcccgt ccggcaaagc tttgcacgcc aggccacgat ttacgggttc gactccagca
     4741 acgcagcgga aagcgctagg tttgcagact ctgagcaact ttacagccca gtacgcctgc
     4801 gcccaaaatg ccgatggtaa aatcttcccg atgatattca ggcagcggtt gccaatgcga
     4861 actattttgc tggatgcgat aatcgtcaaa acgtcgaaac caatgcagca cctgactgac
     4921 agcatattcc tgcatttgct cgcccatacc ggtatcttcc aggcgaaaaa gtggaacaga
     4981 agggttcagc atttcagggt gtgcctgtag cttgctcaaa atagaatcaa caccggcccc
     5041 gagtgcgaac accgctttaa gatcgcgccc tgccagcatt tcaacaggag gatgccagac
     5101 taaaacataa tcagcagagt cattatctcc gcttttccat gccctgactc ttgcctgagg
     5161 aatagctttg cgcagtgcct caatccacca ttgggtatcg aacgttgggt gataaaagat
     5221 gatatccata ctgactcccg aaaagcgttg tgcgaaattt attcgcactt atcgttatga
     5281 tctacaaagg ccaccagcat aacaaatccg tggtcggtgg caaaaaaagc agatttcgct
     5341 tattaaaacc acacattgat tgaatgtttg aataaacgcg cgattttttc aaaaagtttg
     5401 ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca ggaacgacaa
     5461 tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat acggcaacgt
     5521 atcgggggtt cgaatccccc cctcaccgcc agattataag tgcatgttat tgtttaacat
     5581 gcgctttttt atttttcctt ccagacagta tgccatcggg tatgccatct gaaaccggtt
     5641 tatatgcccc gcgatcatat cttatcggac aatttccgat ctttgagcat actggcaaag
     5701 ctatactgcc agcatatcag gatagtgttt tgcgataata tcattcagtc tgtcaaccag
     5761 ttctggtttt ctgaacgtga tatgcgcgga gcccttctta aagtatctga tgctgaacat
     5821 ctcatcttca tagctctctt taccctgaac agactgaatg tgatcatcca gacgaagggt
     5881 gatgtttccc cggttgtcgg ggattggttt accgctgaac aggtgcaaca ttctttccag
     5941 atcggcgagt cggtcagcct gttgtccggt gataagatga aatctatggt cacccccgtt
     6001 tttgcaacac tgatttttga ttaatggttg gcttgcttaa atctatccgg cgtctctgtg
     6061 ggcaaggatg cccgcgcccc gatgagttcc gcacctgata atcctaaaaa tcccctcggc
     6121 ttcagtgagc catttcatct gttaggtttt taatcaggtc gatttgccgt tcatgtcatc
     6181 aataatcagt cttcgcaaaa ccagaaaggt aacttgtatc ttattgttta aaataaacta
     6241 attgagttat atcagcatct atatcaggcc tgatattctt ttttctgagt agtgattgcc
     6301 caggcaattc gagccagttt atttgccaga gcgcaggtaa ccacattcga atgcttcttg
     6361 ttgagttgct cccgaaccca ctcagcaagt tttccatgct tgcgttcaag tcgcattatg
     6421 aatgagcggg cacactgaac cagtaaccgg cgtaaatttt tatcacctcg cttactgata
     6481 cccaacagag tcgattttcc tccagtgcta tactgccggg gaacaagtcc tgtggaagct
     6541 gcaaaatccc tgctgcatga gaactgtttc ccgtcaccaa gttgcgacga tagtaagctg
     6601 gcagtaatcg gaccaacacc aggaattgtc ataatgcgct gagcggtatc atcagcgttg
     6661 attgactgac ttaattctga ttccagctct gctatttgct caacaaggta aaagtagtgg
     6721 gtatgcaacc tcaccaaaag gcggctcaga tattcgggga tttcgtgttc agccaacacg
     6781 agagacaacc gttttattac agcatcacct atcggcaggc tgattccaaa ttccagcaag
     6841 aaaccgtgga tctggttggt tgttttcacc ttatctctga tgagcgattc tctgacccgg
     6901 tgtaacgccc tcatggcctg ctgggtctca gttcttggct ggacaaaacg cattgatggt
     6961 cgtgaagccg cttcgcatat ggcttctgca tcaacaaaat cattcttatt acttttgaca
     7021 aacgggcgaa cgaactgtgg agaaatcagc ttagtttcat gcccaaaatc agagatacag
     7081 cgagccataa agtgtgcacc cgcacaggct tccattacaa caactgaggg cggacatgtc
     7141 gccagaaact gcatcagttt agtacgggta aattttttac gcagaagagc cttaccagac
     7201 ttgtcttgac aatgaatgtg gaaggagtgc tttccgagat cgataccaat aagtgtgatg
     7261 ttctgcatga tggtctctcc gttaaaggga tttaccttaa cagcgtactg ctgttaaggt
     7321 gggggtgacc atatcattat ccccaccggt cccatctcac cagattgttg acgataattt
     7381 tactgccaaa tttacagggg caattggttt tgtaattcca gttcagccct ctgaagacgt
     7441 tgatcacccc tcgctcaaac acctcatcct tattctggtg cagttgttca aatgtgctca
     7501 gaatgttcgc ttcgctgatt tccggaaaat tatcgtactc cagtgaccgg taccatgtat
     7561 cacgggcctg cgtatccatt aatgacagca tcccggaacg ttgcatcagg tcacgccaga
     7621 tttcgcgatc aagattccgg gtaatggctt tcattgctgt ttcgggtttc tccatcagcc
     7681 agcagccgca gcgaaaatcc tgtttcatgg cccagtccgg ggcagtttta cctccgatgc
     7741 tttccgtcag caccgagatt tcagccagtt gtcggataag tgtgtcgatt tgcaacagtg
     7801 tactctttcg tacagccaga atgtggcgaa tgctggttga gcagataacg tccgtatgcc
     7861 cgttgaccac ttccggacgg gagattgtga tatctgacat aatcgatatt cctttttgca
     7921 gataaatgaa aacgccagcc ctgtgcaggc tggcgtcaga ggaatgaagt ctggtgagtg
     7981 agcttcaccg gttctggtgc agaagctgca gatgacgcag ggtacggaca tcctcccggg
     8041 cctgttcgat acagggcaac agtgctgctg aatccgtttt atcctcatcg ttgtcaaaga
     8101 taattcccga tccgcagtcg atattgtcct gcagccacgc aatcagaata tccagcgctg
     8161 tttccgtggt taatgatttc atgttgttcc tggttaccag tcgaaagtgg gtaaacccgg
     8221 caggcattca gcgctggcat ccagatgaat gagactgacg ccataacgcc ggatgagtgt
     8281 gacgaccaga cgacggaacg tttttgacag ccccaggcgt ttaagccgca gaaccgggta
     8341 tgaccacgca tcggtacgta acagatatcc gctacctgta aaatgaatcc attctgattc
     8401 accgaagtca ctggtctggt gtgacagcga gtacaaccag gcgttgtccg tttccgtgat
     8461 atgtgcggta ctgcagcgta tgccggtaag ggtagcaaat ggtggcgtgc attctgctga
     8521 ggtctggtca gattcatcaa caacacgaag ggagtagccg ttatcgcaga ccatgttaat
     8581 cagttcagcg agctcaatac catcgacgtc aacgacaatg cgccctatgt tcagtgcctg
     8641 tacgttaacg ctgtcggctt ccagtgtcag ggccagtttc atcatttcgc ctccggatac
     8701 ttacccaggg taatgttatt taccgttctg taattgtcgc gggtcatcag gccggtcgcc
     8761 cggcgagccc ggaggatatc aatactgttt attaactgag agcggggaca ggtgctgaat
     8821 cccggctggt cggtacgcac cagcgcgtat ttttccacga gaaaattaac tgcatcacac
     8881 agtgaaatgc ctgcctcaat atgctgctca atcacacgtt catcgacaaa cggtgtgtca
     8941 ttcagtgtga ggccataatg ctggtccagc agtcggatga gcagtgtctg ccagatggtg
     9001 acgggagacg ggcagcgaga tacctcccgt acatgtgtgt cggataatgt tttcatctta
     9061 agttctctgt aactggtagt gctaaaggtg agagcttcgg ggttatgagg tggttgcggg
     9121 tgctggtgtc ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcttc
     9181 gcaggtcagc ccttttgcat acagcgtgac ggtatgctga tatcggggat tcagttcacc
     9241 gctggtgagc atgagttcca gttgtttcat cagcagcgga aaggcctggt ccagatggta
     9301 tgcatctgcg tcgctgaagc ggcctctgat accggcgcgg tcggcaaggt aatacaggcg
     9361 gttaccctct tgcaccagac gagcgccaaa acagggcctc actgtgcagg gaagccccca
     9421 ccaggggcgg tcgtgattgt catcgggatg agttatcccg gagagtttgt ctgacacgat
     9481 aaaatcctct ctgaaaagtg gttaagaatg gtttaacgat gacaggtcag ccggcgcgct
     9541 gaccggtttc ggtgaagatg tatgcattga tgagaatggc ctcatccaca gcggcgtctg
     9601 aggtcatctg gtcgtactcg tttcccgggt gacggaacat cagttcaggg tgacactcat
     9661 cagccggaca tacgggccaa aactgtcctt acggcgttct gcaaacacgg caagaacgcc
     9721 gggaatatcc tgcacttcac gaccagtata cgcttcagca ctgccgtgcc agcgatactt
     9781 accggtacag aacggaaaaa gacgggatgc aggatgctgt tggtgaatac gcatggcttc
     9841 accacgggtg atgattctca tgatgtgata cctctgaaga cagaagataa aagtgaaaac
     9901 aggtgtgatg tggttgtgac gggttaaagc agaccatgtt cggcaaagga gaaaacctgg
     9961 ttgccaccga ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc
    10021 agacgttcgg tgataagccg gtctgcctta ctgggtgtga cttcaccgga cgggtgattg
    10081 tgcgccagca ccacggcagc ggcattgtgg tacagggcgc gtttaatcac ttcccgggga
    10141 tgaacttccg tacggttgat ggtgccggtg aagagggttt caccggcaat cagctgattc
    10201 tggttgttca gatacagcac ccggaactct tcccgctcca gtcccgccat gttcagaatc
    10261 agccattccc gtgccgcacg ggtggaggtg aaggccacgc cgggttcatg aagatggcgg
    10321 tccagggttt gcagggcccg cagaatgaga ctgcgctcgc cgggcgtcat gtttccgggc
    10381 agaaaggaaa gctgctgcat tgtccctgcc tccattcagt cgatgatgcg cataatggcg
    10441 ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt aataatggac cgtcatggca
    10501 taacactccg tacgacaggc atgatgactg tacgtcatca gacaggcagc aatgccggcg
    10561 gcttccgggc tcatttcagc gcggttaccg ttcatggcat tgaacagtac ccatgtttcg
    10621 tcatcatcgt catccggttc gggtgccata aatgccccgc cgttgttcag ggtgtacaga
    10681 ttccagatac caccgcagta gtcttcacac agacggtcca tccagccgaa gacacggggt
    10741 tccagggtga cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt
    10801 tcatcaggga cgggagtggc aaccagttgt tgcaggccag tctccggtgc acaggccgaa
    10861 gcggtggtgt tctgagacaa tgttttcatg tatgaaatcc ttgtaatgtg aataaatgag
    10921 agggagtaaa ggtcgggagc tggcaacatc acgttcatca ctgaccgtga tgacccggta
    10981 atatcaggga tgaatgatat tcggctgaaa ttccggacag ccgtacgcgt aaccgttccg
    11041 gtcacagacg gatggccaga tgccattgcc tgtatactca gtctgcacag actgatttct
    11101 ccgggacggt aaagcagacg ggtacaccag gcgcacaggt catcatcaga aatggaacga
    11161 caccccggca gcgcctccgg ggcgtcgggt gattctggtg cctgaatcac gccttcaggg
    11221 acatgctgcc tcagcgcagg ctctccagca gcgtttctgc cattacccac aatgcgcggt
    11281 tgagcttaat atcggtgtcg atgttgtgaa tggcacgggt gtggatacgt tttccttttg
    11341 cactgcggcc ggaaatcccg cctttcagca tattctcctg gatggtctga tatgcgctcc
    11401 acaggtcttt accgtaatcc tccctgcgtc gtggtgtcag aatgtcagcg gtggtgactg
    11461 gctgatgttc gtcaccataa cggtaagtca gtgccgcctg tgccagcgcc tggcgtgccg
    11521 gtggcggcag gaccagcgac tgcatggcat cacgcttctc ctctatccgg tcaaacacgc
    11581 ccaccacctc gtaagcccct tcaataactt tctccaccac atttccccgg tgtggaacac
    11641 gcaattctcc cagagactga ccgcagacca gtccattggt acaaatggca cgaaaatatc
    11701 cgggtaacat ctggtagctg gatgaaccgt catgagagtt gagcagaata atttcaggga
    11761 catgctgacc ggttatctgt ccggcccgcc gcagacgcag catgtgtttt gtgtattccc
    11821 ggcgacccgg gtcacgcaca cgtgtctggc aggcgaagaa tggctgaaag ccttcccgct
    11881 gcaggctttc cagtacggtg atggtgggag tgtacgcata gcgtttactg cgggaagtat
    11941 gccggtcttc accgaaaata ctgggaacat agtgcatcag ttcttcgtgt gtcagcggac
    12001 ggtcacggcg tatctggttt gcagcataac caaaacgact ggctagtcgc ataatttgct
    12061 ccttatcggt ggttaagatt tactggtgta ataaatgaaa aagccacgtc tcccggagaa
    12121 gacgcggcct gatggacgaa attaatgagg tttattgtct gagaagaccg tcactggcga
    12181 accttccggc gtcagtctct gcgttgcgga taacccgacg gaaatgagtt ccctggtcat
    12241 cgtcgataaa cacattgcgg taagctgttg tgatgacaac agcctgctcg cgtgagaaag
    12301 ggacttcggg caataaacgt gacatacaac cagacctgta tttggttccc tgaaaatata
    12361 agtccctgtc atcatggcag agccgtattt acttttgata tcaataaatt aataacgcga
    12421 agatgaaccg cataacttac gcctttaatt tgttttgttt acgtagtccc tgaccaggta
    12481 cgcgaattcg tgaacgccag tttcaccaaa ccaaatcagg taaccagggt cgatgttgta
    12541 atagtaacca acgactttat gcgtttttgg gttatagagt gttacgatat agtcccagcc
    12601 gcaggcgtgg gctttacaac cgccaccttc tacccaggtc gttccgttaa tgtttacagg
    12661 atacgcacca ccatcaggac catccatata gccgtcttcc ccaaacattc cgtttaaccc
    12721 aacagggttt aatgctttgt cgatagcagc tctgtaggta gcgtcacctt cgtataactc
    12781 gtacgttgtt ggacatgcac caggctggtc agcccgggct tttgagtcgc atttaagtgt
    12841 ctgatttaca gtggcggcgt ttgtgtcagg actt
//
LOCUS       DAJTNV010000104        12871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNV010000104 DAJTNV010000000
VERSION     DAJTNV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO199_003252"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO199_003252"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5537185.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO199_003253"
     CDS             complement(1806..3224)
                     /locus_tag="OO199_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5537186.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO199_003254"
     CDS             complement(3378..3722)
                     /locus_tag="OO199_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5537187.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO199_003255"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO199_003255"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5537188.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO199_003256"
     CDS             complement(4712..5647)
                     /locus_tag="OO199_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5537189.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO199_003257"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO199_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5537190.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO199_003258"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO199_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5537191.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO199_003259"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO199_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5537192.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO199_003260"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO199_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5537193.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO199_003261"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO199_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5537194.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO199_003262"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO199_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5537195.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO199_003263"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO199_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5537196.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO199_003264"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO199_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5537197.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO199_003265"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO199_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5537198.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3391 a         3288 c         2943 g         3249 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata a
//
LOCUS       DAJTNV010000105        12862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNV010000105 DAJTNV010000000
VERSION     DAJTNV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="OO199_003266"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="OO199_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5537199.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="OO199_003267"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="OO199_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5537200.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="OO199_003268"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="OO199_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5537201.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..3249
                     /locus_tag="OO199_003269"
     CDS             1993..3249
                     /locus_tag="OO199_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5537202.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3542..4783
                     /gene="agp"
                     /locus_tag="OO199_003270"
     CDS             3542..4783
                     /gene="agp"
                     /locus_tag="OO199_003270"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5537203.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4821..5048)
                     /locus_tag="OO199_003271"
     CDS             complement(4821..5048)
                     /locus_tag="OO199_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5537204.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="OO199_003272"
     CDS             complement(5069..5665)
                     /gene="wrbA"
                     /locus_tag="OO199_003272"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5537205.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6038..6145
                     /locus_tag="OO199_003273"
     CDS             6038..6145
                     /locus_tag="OO199_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537206.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="OO199_003274"
     CDS             complement(6227..7555)
                     /gene="rutG"
                     /locus_tag="OO199_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5537207.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="OO199_003275"
     CDS             complement(7576..8070)
                     /gene="rutF"
                     /locus_tag="OO199_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5537208.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8081..8671)
                     /locus_tag="OO199_003276"
     CDS             complement(8081..8671)
                     /locus_tag="OO199_003276"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5537209.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="OO199_003277"
     CDS             complement(8681..9481)
                     /gene="rutD"
                     /locus_tag="OO199_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5537210.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="OO199_003278"
     CDS             complement(9489..9875)
                     /gene="rutC"
                     /locus_tag="OO199_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5537211.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="OO199_003279"
     CDS             complement(9887..10579)
                     /gene="rutB"
                     /locus_tag="OO199_003279"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5537212.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="OO199_003280"
     CDS             complement(10579..11670)
                     /gene="rutA"
                     /locus_tag="OO199_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5537213.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11958..12596
                     /gene="rutR"
                     /locus_tag="OO199_003281"
     CDS             11958..12596
                     /gene="rutR"
                     /locus_tag="OO199_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5537214.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3275 a         3235 c         3274 g         3078 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgactg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccgcgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagcctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag atgatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat
     2581 aaaacatatg gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc
     2641 atactggttt taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag
     2701 caagtatacg aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat
     2761 acggcattct ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct
     2821 gacaacaaaa ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa
     2881 caattcattg atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata
     2941 tttaatgata acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaagcg
     3001 gtgaagaatt ttcttgctgt tctttcagat agcatttgct cattatttat tgttaatagt
     3061 aatgtggcat caatctcctt gggaaatgaa tccttttcaa cagatgaaga tcttgagtat
     3121 ggttatttaa caaacactgg caatcattat gatgtttacc tccctcctga acttttttct
     3181 caggcttata agttaaaaaa taaggaaatg aatgcgcaac ttgactattt aaatcgttat
     3241 gcaacttaat ggcaaaggca tatgctaaaa accattgtta ttagtctcac acttttttat
     3301 tggtaaatat tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc acaggtgcaa
     3361 ttatcagcgg cgtacgcgag gcaggggcta atcaggcata gtttgcgtca aaccttgcct
     3421 gtttttgaag atgtatatag aaaaacaggc gttcaacaag ccattttgcg aacctgttcc
     3481 cggaaaaaag tcatatttct gtcacactct ttaatgattg ataacaaaag aggtgccagg
     3541 aatgaacaaa acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc
     3601 tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca
     3661 taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa
     3721 atggcccgaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt
     3781 gtatatgggc cattacatgc gtgaatggct ggcagagcag gggatagtga aatcggggga
     3841 atgcccgccg ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgtcgctac
     3901 cgcccagttc tttattactg gtgcattccc tgggtgtgat attcctgtgc atcaccagga
     3961 aaaaatgggc actatggacc caacctttaa cccgatgatc accgatgatt ccgccgcatt
     4021 cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag
     4081 ctaccagcta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca
     4141 acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg
     4201 tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta
     4261 cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa
     4321 ggtattgtcg aagctgaaaa acggttacca ggacagtctg tttacctcac cggaagtggc
     4381 gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcgc
     4441 cagtgctccg aaaatcacgg tgttagttgg gcacgactcc aacatagcct ctctgttaac
     4501 ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg
     4561 caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga
     4621 atatgtgtac cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc
     4681 tgcgcagcgc gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc
     4741 gatggataag tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg
     4801 agaagcgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa
     4861 agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcact accttcttcc
     4921 caaatctttt ctgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt
     4981 tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga
     5041 gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga
     5101 catattcccc ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt
     5161 caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa
     5221 ataattcctg cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg
     5281 tccaggtgga tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc
     5341 tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg
     5401 tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt
     5461 aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt
     5521 caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca
     5581 ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat
     5641 aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag
     5701 cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc
     5761 acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt
     5821 ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata
     5881 tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgt ccgcgtcagt
     5941 tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct
     6001 atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc
     6061 cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggcaagag
     6121 cagtcacggc aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta
     6181 agctcgtcaa aaagtcatca ccgccggtaa cccggcggtt ttctgtttat ggctcctgat
     6241 gaacaacttc tggcggtgga acgtcaacca accttctgct taataacgca ttgagtaaaa
     6301 tcgcgccaaa ggttgctgta ccgatccctc ccaacgtaaa accgccaagc gtgagagcaa
     6361 aatcacccgc gcccagcacg aaggttactg cgaccataat caaattagcg ttctggctta
     6421 aatcgacacg gttttgtacc catatccttg cgcctgcgac ggcaatcagc ccgaacacaa
     6481 caattgatgc accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg
     6541 gcgaaaagcc caacagcata gcgatgacgg cagctgcaac aaacaccagc gtcgagtaga
     6601 cttttgtcac ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga
     6661 cagagccgga aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt
     6721 ccatattgcg tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca
     6781 gaatcaccgc cacgggcgca atcaacatca tcgcttggcc attaaaacca ggagtggaaa
     6841 aatgcggcag accgaaccag gcagcatagc tgacgagagt aaaatcgacg gcttttccca
     6901 gccctaaaac gttggtcatc acgccataca gcagacaggc gacaattaat cctacgagaa
     6961 tcaataaccg ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg
     7021 tcattaccgc catccagcta tcaaaggccg aagccgatac acttttcact gcgataggcg
     7081 ctaagttcag gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt
     7141 caatccagcg tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac
     7201 aggcgataat cccgcccagc gcgatgctga tattcgggtt aatgccctga ccgttaaagc
     7261 ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc
     7321 gcccgccggt aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat
     7381 tgggatccag ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt
     7441 gttgaacgcc cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc
     7501 cgctctctgt agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct
     7561 gtctccttaa ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca
     7621 caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc
     7681 gtgggtgccg acgctcacca cctggctgat acggcagtca aacgaaacca gagcctcttc
     7741 cagttgcggg catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat
     7801 gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac
     7861 acacagcgtt cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac
     7921 cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg
     7981 cccggctgga ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc
     8041 gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt
     8101 cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat
     8161 taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac
     8221 ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat
     8281 aagccgcctg catggaactg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg
     8341 taaaccaact gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac
     8401 tgtcccaggc gacgatagcg gtgacgggcg cggtcagggt tttttgcaga ttgccgctgg
     8461 aaagcgccgg gcgcagacgt tcttttcctt ctgcagtgcg gataaacacg atccgtgccg
     8521 gagaacagtt agctgatgtc ggcccccatt tcatcagggc ataaagctcc tgtaacgtct
     8581 catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc
     8641 taagcgcacc tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt
     8701 gatgtaacag gctggcaagc ccgttgagta acagagcatt aaacgtttcg ggatcggtca
     8761 cgttgcaggc gtgtccgcca tagggcatca ccattttctg gctatcgggc agggcggcat
     8821 gaagttcact ggaacatgct gttggcacca gcagatcatc actggcgcag atgatttgca
     8881 ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc
     8941 gacgtagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcca
     9001 ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca
     9061 cccatgcttg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg
     9121 tatgggcgtt tattcgtagc cagccgttaa cgctgaccag catagttacc gacgcgggat
     9181 aatccagcgc cagctgcatc cccaccagcg caccgagcgc atggccgacc accgcgtaac
     9241 gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt
     9301 aatcttctgc cagtgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct
     9361 gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac
     9421 ccgaaatcaa caccactacg ggtgcatcag cataaggggg aggtgagagt gaaagtttca
     9481 tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt
     9541 accagtccgc actgaatgta gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag
     9601 atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca
     9661 tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg
     9721 gtttgcgcct ttgggtcatc ggcaaacagc acgttattat gttgatcaaa agccagcgta
     9781 ccggagacat acaccacgcc atcagccagc gtgccgggaa cgaagggggc cagcggtgcg
     9841 ctgctgccag cgggaataat tacggatttt ggcatcgcta aactccttaa gcgatacgag
     9901 caaaggacgt gggagaaagc gcgtcgcaga acgtttcgac gtcgctgacc cagccaaaaa
     9961 aggtttcgat attgaacaac gcggctttct gtgcaaattc cggccccgcc tggtgtgttg
    10021 cgtcttccag caccacgccg aaatgctcca gaaaaaagcc atcgcgtagc gtcgattcga
    10081 cgcagacgtt ggtagcgata ccggtgaaaa ccagatggcg tattccacgg ctgcgtaaaa
    10141 tgctgtccag cggcgtattg aagaaaccgc tgtagcgcgg cttcggcagc acaatatcgc
    10201 caggctgcgg caccagttca tccaccagtt gataatccca ggagcctttc gccagcaatt
    10261 tcccctgcag ctgcggctgc ttacgcatgg ttttcagggc gttcgattta tgaaaattcg
    10321 gtgagccggg gccgccagcc tcgacatact gttcatccca gccattttga aaccagatga
    10381 tcaacatccc tgccgctcgc gctgcggtca cagcggtttg aatgttggca atgaccgggc
    10441 gagtggttga gacatcaaac ccggcgagat ctaagtagcc gcctggcgtg gcataagcgt
    10501 tttgcatatc caccacgatc tgcgcacttt gctgcggatc gaaggtaatg gcttccggtc
    10561 gagcggttaa ggttgtcatc atgccacctc ctgagtcagc gcagagagat gggcgcggca
    10621 ctgcatcagt ggttgaatgc gctcgccgaa ggtttcgatt cccgacagaa aatcatcgaa
    10681 ggtcaacagc acgccttctg caccaggcac gcttgcgact tcatctaaca tgcgcgcgac
    10741 actggcgtaa gaaccgacta acgtccccat attgatgttt accgccgaag tgggatcggc
    10801 catctgacga acgttggtgt cagtaccgga gcgggtatct ttctgacttt gttcggttag
    10861 ccagcttaac gcctcttcat ccgcgcccgc tttgtagtgt tcccatttgg cgcgagtggc
    10921 atcgtcggtt tcatcggcaa tcaccataaa caacacataa gagccaacgt cgcgtccggt
    10981 ttgctctgtg gcctgtttca tccgcgcagc ggtcggggcg aaagccgtgg gtgtatttac
    11041 gcctttgccg aaacagaagt tgaaatcggc atactgggcg gagaacgcca tgccagcgtc
    11101 gctttgcccg gcgcagatca ctttcatggg gatactcggt tgcggactga cgcgacaatc
    11161 attcatggtg aaaaaatcgc ctttaaaatc gcttttcccc gtgccccaca ggtcgcgcag
    11221 cacctgaaca tattcggtga gatagtcgta acgacgggag aaatagtcat cgccaggcca
    11281 gatacccatc tgctcatact cgggcttttg ccagccagtc acgaggttga cgccaaaacg
    11341 cccgccagag atggagtcga tggttgcggc catacgggcg acgattgccg gaggtaacgt
    11401 taaggtggca gcagtggcgt aaatctgaat gcgcgaggtc acggctgcca gacccgccat
    11461 caaggtgaac gactcaaggt tgtgatccca gaactcagtt ttgccgccaa agccacgcag
    11521 tttgatcatc gacagggcga aatcgaaatg gtaatgctcc gctttttgcg caatggcttt
    11581 attcagttca aaggtcggca tgtactgcgg cgcgtgggtc gaaatgagcc agccgttgtt
    11641 gccaataggt acgaatacgc caattttcat catcaacctc tcttcgtctc gtaaagtgaa
    11701 agtcagacgg ggcgctgcat cctgcatatc cttttcagcc gcgtattggc ttgtttgcaa
    11761 agcggatgcc agtttttaaa aagtgaatgt tattaatgtg ttagcattgt gttatctaaa
    11821 atatctggta aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg
    11881 cgcgatgaga gtgcagaagg tcgaggccgg gcgggggttt tgctatcctg ttaccaatct
    11941 acaagagggg agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg
    12001 cagtaagcgc gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg
    12061 gttttcacgg cacaagactg gagcagaccg cagagttggc gggtgtttca aaaaccaatc
    12121 tgctgtatta cttcccgtca aaagaggcgc tgtatattgc cgtgctgcgg cagattctcg
    12181 atatctggct ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgataa
    12241 aagagtacat ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcgcgcctgt
    12301 tctgtatgga gatgctggca ggcgcgccgt tgttaatgga tgaactgacg ggcgatttga
    12361 aggcattaat tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg
    12421 cgccgattga tccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg
    12481 atttcgcccc tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca
    12541 atcaaacggt tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat
    12601 gacggaggag gttgtaacat cctccggcta cctgtttaac cgatcgtcat taaactggcg
    12661 ttaccgccag cggcagcggt attcacactc agcgaacgtt catcgatagg aattaaaacc
    12721 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat
    12781 atgaacggaa ccgacgatta aactggatgg ctctgattga ttcagggtat gaatggcggt
    12841 tttttgctcc gtttccctca aa
//
LOCUS       DAJTNV010000106        12425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNV010000106 DAJTNV010000000
VERSION     DAJTNV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="OO199_003282"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="OO199_003282"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5537215.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="OO199_003283"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="OO199_003283"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5537216.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO199_003284"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OO199_003284"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5537217.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="OO199_003285"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="OO199_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="OO199_003286"
     CDS             3602..3958
                     /locus_tag="OO199_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5537218.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="OO199_003287"
     CDS             4285..4944
                     /locus_tag="OO199_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5537219.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="OO199_003288"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="OO199_003288"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5537220.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO199_003289"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OO199_003289"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5537221.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="OO199_003290"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="OO199_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO199_003291"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OO199_003291"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5537222.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="OO199_003292"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="OO199_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5537223.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="OO199_003293"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="OO199_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="OO199_003294"
     CDS             10287..10628
                     /locus_tag="OO199_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5537224.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="OO199_003295"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="OO199_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="OO199_003296"
     CDS             complement(12063..12344)
                     /locus_tag="OO199_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5537225.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3081 a         3033 c         3141 g         3170 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aataatctga
    12421 ggtgt
//
LOCUS       DAJTNV010000107        12212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNV010000107 DAJTNV010000000
VERSION     DAJTNV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..482)
                     /locus_tag="OO199_003297"
     CDS             complement(<1..482)
                     /locus_tag="OO199_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5537226.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     "
     gene            complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OO199_003298"
     CDS             complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OO199_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5537227.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OO199_003299"
     CDS             complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OO199_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5537228.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2448..3314
                     /gene="folD"
                     /locus_tag="OO199_003300"
     CDS             2448..3314
                     /gene="folD"
                     /locus_tag="OO199_003300"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5537229.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3316..3528
                     /gene="ybcJ"
                     /locus_tag="OO199_003301"
     CDS             3316..3528
                     /gene="ybcJ"
                     /locus_tag="OO199_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5537230.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3636..4145
                     /locus_tag="OO199_003302"
     CDS             3636..4145
                     /locus_tag="OO199_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5537231.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OO199_003303"
     CDS             complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OO199_003303"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5537232.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5740..6234
                     /gene="ppiB"
                     /locus_tag="OO199_003304"
     CDS             5740..6234
                     /gene="ppiB"
                     /locus_tag="OO199_003304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5537233.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6237..6959
                     /gene="lpxH"
                     /locus_tag="OO199_003305"
     CDS             6237..6959
                     /gene="lpxH"
                     /locus_tag="OO199_003305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5537234.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7077..7586
                     /gene="purE"
                     /locus_tag="OO199_003306"
     CDS             7077..7586
                     /gene="purE"
                     /locus_tag="OO199_003306"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5537235.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7583..8650
                     /gene="purK"
                     /locus_tag="OO199_003307"
     CDS             7583..8650
                     /gene="purK"
                     /locus_tag="OO199_003307"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5537236.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8787..9680)
                     /locus_tag="OO199_003308"
     CDS             complement(8787..9680)
                     /locus_tag="OO199_003308"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5537237.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9677..10324)
                     /locus_tag="OO199_003309"
                     /pseudo
     CDS             complement(9677..10324)
                     /locus_tag="OO199_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10327..11559
                     /gene="allC"
                     /locus_tag="OO199_003310"
     CDS             10327..11559
                     /gene="allC"
                     /locus_tag="OO199_003310"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5537238.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11570..>12212
                     /gene="allE"
                     /locus_tag="OO199_003311"
     CDS             11570..>12212
                     /gene="allE"
                     /locus_tag="OO199_003311"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5537239.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2968 a         3154 c         3114 g         2976 t
ORIGIN      
        1 aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc aaagttaatt
       61 gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg ctgggctaac
      121 tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt aaaacagggc
      181 attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac gatatcctgt
      241 gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc aggttgatga
      301 ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa taaaaatgcc
      361 gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag agatgaataa
      421 caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt gggtattttc
      481 ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc tctgaccggg
      541 gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac tcctgagggt
      601 aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt ttggctgccc
      661 acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt gatatagtta
      721 ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg taattgaatt
      781 ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt cctgccgttc
      841 aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag agactcacga
      901 tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct tttgggttca
      961 ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct gctgttttca
     1021 atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca taccgcagtc
     1081 tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc tccggcagca
     1141 tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt tattttagtc
     1201 atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat aaagaaatga
     1261 ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
     1321 agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg gtacattact
     1381 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
     1441 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
     1501 cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt tgagaagcta
     1561 ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc gacacccgtt
     1621 gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat tgcatcagcc
     1681 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
     1741 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
     1801 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
     1861 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
     1921 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
     1981 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
     2041 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
     2101 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa cacaaatata
     2161 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
     2221 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgta
     2281 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
     2341 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
     2401 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
     2461 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
     2521 ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
     2581 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
     2641 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgatg
     2701 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
     2761 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
     2821 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
     2881 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
     2941 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
     3001 ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
     3061 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
     3121 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
     3181 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
     3241 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
     3301 cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
     3361 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
     3421 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
     3481 ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc cctttcccgg
     3541 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
     3601 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
     3661 ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt gccggaatca
     3721 tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt gcttacggca
     3781 atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt gtcccgctct
     3841 tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc tggctgtttt
     3901 taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc ggtaagggcg
     3961 ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag
     4021 tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg attatttccg
     4081 aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac
     4141 gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga cgccaggtgg
     4201 tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca cgtgccgcat
     4261 ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa
     4321 tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt
     4381 gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg
     4441 ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac agcacggaat
     4501 aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt
     4561 cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag aggcgctcca
     4621 gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc
     4681 ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg
     4741 taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt
     4801 gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg atttcgttct
     4861 cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg ttacccagct
     4921 gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg ccccacggag
     4981 acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc
     5041 gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga tcctgacgcg
     5101 acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgcca
     5161 cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt
     5221 gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca
     5281 tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca ttggcgcgtt
     5341 tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga
     5401 aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac
     5461 agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg tgaataggct
     5521 taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt
     5581 agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata
     5641 cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa
     5701 ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat
     5761 cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc
     5821 ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc
     5881 tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg
     5941 atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc aatggcacgt
     6001 actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc
     6061 ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt
     6121 gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa cggtatgcac
     6181 caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg
     6241 cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt
     6301 ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt cttggcgatc
     6361 tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag atggcggcag
     6421 cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc aaccgtgatt
     6481 ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg gaagaaaagg
     6541 tgctcgaact ttatggtcgc cgggtgttaa ttatgcatgg cgacacgctg tgcaccgatg
     6601 acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc
     6661 tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg aacagcaaag
     6721 aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct gtggtcagtg
     6781 cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc ccggcggtgc
     6841 atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc tggcatacgg
     6901 aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt ccgttttaaa
     6961 aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg
     7021 ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt
     7081 cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta
     7141 ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg
     7201 tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg
     7261 gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc atgattgccg
     7321 ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg
     7381 atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg
     7441 gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata
     7501 aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa gtgctggaaa
     7561 acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg
     7621 ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg tcgggctgga
     7681 cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg
     7741 gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga accgcgatgt
     7801 gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc tccacctgcc
     7861 gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt
     7921 aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggag
     7981 tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat gtattgtcga
     8041 gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct ttgatggcag
     8101 caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt
     8161 cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat
     8221 tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg
     8281 tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaacgg
     8341 tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca
     8401 accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga
     8461 ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg
     8521 taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga
     8581 agccttaatc ccgctgctgc cgccagagta tgcctgcggc gtgatttggg cgcagagtaa
     8641 gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt
     8701 accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt
     8761 gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc
     8821 agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg gcttctgaca
     8881 taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggagct
     8941 aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat
     9001 accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac
     9061 gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc cgtcacaggc
     9121 acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct
     9181 tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca
     9241 tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa
     9301 accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg
     9361 cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc
     9421 atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata cggttctacc
     9481 tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca
     9541 atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg
     9601 cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga
     9661 gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca
     9721 gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg
     9781 tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt
     9841 gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg
     9901 aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac caggccgcca
     9961 gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct ttacccagcc
    10021 agtaccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga cgaaactggc
    10081 gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa agtccggttt
    10141 cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc ggcgtaatac
    10201 gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag
    10261 ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg aattgcgtgc
    10321 ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct ttcctctttt
    10381 ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg gctggaaacc
    10441 cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt cgatgaagtg
    10501 gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt tctgagcggt
    10561 tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg cgcgctggcg
    10621 gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg tacggtcgaa
    10681 gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg gggcagtaaa
    10741 aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc caaaggaaat
    10801 agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc actaactccg
    10861 cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt gctggaaagt
    10921 aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta tacggtgacg
    10981 ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt
    11041 tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat gggcgatccg
    11101 ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt ggtgccgggt
    11161 aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg cgatttcacc
    11221 caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg tattgatatc
    11281 gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc caccctgaca
    11341 gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc cgggcacgac
    11401 gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag catcaacggg
    11461 atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg ggtcaaaacg
    11521 ttggcactca tgctttatca acttgcctgg cagaaataag gagtcataaa tgggatattt
    11581 aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg ttaaacacgg
    11641 taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg actttgaaaa
    11701 ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg attatctggt
    11761 cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt
    11821 cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat ttgccttaag
    11881 cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta acgcccaggc
    11941 cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag gccatgcacc
    12001 gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag gtatggatga
    12061 tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca tgcatatcct
    12121 ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg aacatggtgc
    12181 ctatattctt tccggtcagg gggtttataa cc
//
LOCUS       DAJTNV010000108        12027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNV010000108 DAJTNV010000000
VERSION     DAJTNV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(104..2443)
                     /locus_tag="OO199_003312"
                     /pseudo
     CDS             complement(104..2443)
                     /locus_tag="OO199_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2377..3000)
                     /locus_tag="OO199_003313"
     CDS             complement(2377..3000)
                     /locus_tag="OO199_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5537240.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2997..4685)
                     /locus_tag="OO199_003314"
                     /pseudo
     CDS             complement(2997..4685)
                     /locus_tag="OO199_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4834..7461)
                     /gene="gyrA"
                     /locus_tag="OO199_003315"
     CDS             complement(4834..7461)
                     /gene="gyrA"
                     /locus_tag="OO199_003315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5537241.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7608..8330
                     /locus_tag="OO199_003316"
     CDS             7608..8330
                     /locus_tag="OO199_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5537242.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8458..10389)
                     /gene="yfaL"
                     /locus_tag="OO199_003317"
     CDS             complement(8458..10389)
                     /gene="yfaL"
                     /locus_tag="OO199_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5537243.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10402..11997)
                     /locus_tag="OO199_003318"
     CDS             complement(10402..11997)
                     /locus_tag="OO199_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5537244.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2779 a         3332 c         2967 g         2949 t
ORIGIN      
        1 aactctgcgc taaaatttct ttttttcatt ggagcacctg tgttgttctg aggtgagcat
       61 atcacctctg ttcaggtggc caaattcagt gtgccacttc acattcgaca ttgaccagtt
      121 caccaggctg gtaatgggtt ttgtccgttt tcacccggat atcaagctga ggaacggcaa
      181 ctttgatccc ggcgttctga aaactgtact gaccattacg ggtatacagc accgaaaaag
      241 tgatgttagg cgcaaaggaa ttgctcactg gtacccgggc ttcatacagg gtatcgttta
      301 aacgttgtag cgttagccag tttgccggat gcgaaagcag cgactgctgt tccacgcgat
      361 cgcgttccag cgtcaataac gcttcatcaa ttggctccgg gaaagtaatc agcatcttcg
      421 cggtttcgcc tggctggtac agcgttttat ccgccacgat atctaccgta ccagtatgcg
      481 ccgtgctgcc cttaccgctg acggcatgac ttaacccagc gagaattaag ccgtctttat
      541 cgcgtaatgt cagattgtag ttgccaggtt tagcgaaatt gacggtaaag gatttgccgc
      601 ctgacggtag ctctccgcta tggctcgtgc ggtcttcgag acgcaaccat tcatacgtaa
      661 caggaacctg ttttgaagat tccagcgcgg cataacggaa cacaaccgac tcgccgctat
      721 tactgtattg tgcggcggta cttaaagagt aatgcgccag gccgcgttca atgaggatct
      781 ctttggtggt ggtgacgcga tacgccgcgc cgtcactggc ggagacggtt aacaaatagc
      841 ggctcggttt atcggcggcg gggagattta acgccacatg accgctggcg tcggacaccg
      901 tttcgctgcc ttccagcgac acggggaaac gtccggcata acgcaaatcg ttaccgacca
      961 ttgataattg ctgagcgcgc aaacttaact gcacgcgggc attttttacc ggctcgccat
     1021 ccgggtagag cagttgcagt ttgccgctga ccgcttcgcc agttttgaac tcttttttgt
     1081 cgagagctaa accaatctcg aaatgtggct tgatgtagtt tgccacgcga aaactgctgc
     1141 tatagacctg attgcggtaa gcaagacgta actcataacc tccggcaacg gcattttctg
     1201 gcaggcggaa acttccctgc ccgccattgc gcgcatccag cgtgacatcg acggtttgca
     1261 acagactgcc gttagcgtcc agcaccgaaa gcttcgccgg ggcgctgacg atgggggatg
     1321 aatgcaacgg atcgcggaac tcgcggccca tcactttaac atcgacacga tcgcctgcgc
     1381 gatatagcgg gcgatcggta aaaatataca agcgggtgtt gtagatttcg ctttcgtaga
     1441 agaagttctc ggagacaaaa acgccgcctt cagcatcctt acccagaatg tatgaacgtt
     1501 ctggcgatat atgttgtaac tgcaaagtac cgctgtcatc ggtcaccccg cgggtcatca
     1561 cgccaagacc gtcagtccac aagatctctg agccgggctt cgcttcaccc tgttttttac
     1621 ccgcggtcca taccagaagc tcgttacctg acactttgct aagcgccacg gtatcggaaa
     1681 caaacaccac cgtcgtcgcc cgatacccac ctaccatcgc ctcgacgagg tacagccccg
     1741 gctcttgttg gccgagagga atataaatat tacccggctg cggcgagatg aaattgctgg
     1801 atgcgccttc cagttttacc ccttgctgcg gctcgaccgg tttagcctgc catagtggat
     1861 aacggaactg ttccaccaag ggatattttt tcagcgggga aaactggttg ttctgtacat
     1921 aacgggaagg tttaataatg gcattgccga gatgtaattc gggtaatgcc tgagtcacat
     1981 tctgccgtga ctgagaagag aaagtacgct gcatcacgcg gcgagatttg ccgtaccagt
     2041 tatcccacag ccaggtcagc gtattgttca gcccgtcgcc cagatattgc ggttgcacca
     2101 caatgcgatg caggtttttc tgctggcgca aaaatgccat cgggtcagga atacgatata
     2161 ggcgaacgtc cacgccgccg tactcttcca tctgatagcg ccgataatca cgccccggcg
     2221 cttccagtcg cactttcgcc tcttcactgc tgctaaaact gctgtcagca agcaaaaaga
     2281 atgttccccc ggcgggcggc gcatagttac tggaaggaag cgaatcatca gctttagcaa
     2341 gccctgttcc cacaagagac agggacaagc acgctgctat cgcgccagaa aatttaaacg
     2401 ataaatgcca atgaaattgg gattggaatc gttgggtatc catcgggtgt ccttccatgt
     2461 cataagttgt tgcagactga ctgcgcgcat tccgttgtca gttttcgtgg cgcttccggt
     2521 gtggtagatg acgtaacgcc ccatccagac cattaagtgc tgggcatcgc cctgatcgaa
     2581 aaaaatcata tcgccaggca gcgcctggtt tatgtcttgg ccaataaact ggctgttgta
     2641 ctgaatcaaa ttaatcgcgg tcacgtaggg gccgtttttc ccgctcccct gattccagtt
     2701 ttgcgccagt tgacgctgtt caggtgttag cgtcatctct ggcggcaaat actggctaga
     2761 taaaccgtta ctttttagcc atttactgtc gtgaactttc agcgtctcgt tcgccgcaaa
     2821 tcgcaccagg cccgcacaat cctgctgata ccagcgtgga cttggcccct ggcggagctg
     2881 ttcttgtgca atgcgtacaa accaggcgcg aaacagcctg gattgttcga cattcagcat
     2941 ttcgctatgg gcaacaacac aacacagcca gcaaatcagc gccagcagcc cgtgtctcat
     3001 agcggctgcc aggttatggg tagccactgc caggcgacac cgggttccat ctgggccagc
     3061 ttcatgacat aacgcggttg ttgagataaa gcgtccagct tcggcattaa taaagtttgt
     3121 gcggcgttat aaaaaaccgg ttcgagattc ttcggcagac tggtcaacgt ttcgttacgc
     3181 agcagtttcg ccacgccttg tggattgata tagagcggaa cgatgccatc agtgggtatt
     3241 acatccacca ttgccgggcg ggttttattc agtgtttgca gggcgttatt aaccagcgtg
     3301 tcatcgaggg agaaaagcag cgttttgttt tgcatcgcca gcgacacacg gaaaaaataa
     3361 tccgacatta attgatcggg ttgcgccgcc tgcgctttcg gatactgacc gtatcgggaa
     3421 ctcacttcgc gacaccagat ttgcgcttcg ccctgctgag tctggcttac cggcaaaaca
     3481 ccttctggcg ctttgctttc gtgcgcacca atattttgcg taaacagttt ccctggcaat
     3541 tgcgcctgtt cggcagtgcc atcaaactga ccgacaaaca gcggggtttg caattttgag
     3601 tcttcatacc agcacagccc cgcggcaccg tctaacgccc cattcagctt gtcgttttcc
     3661 tggctgatgt gcgaaagcat ctcttcggca ataccgtgtg aatacggcac cgccacacag
     3721 aagctggctc cggcaggcat actgttccag accggcgtaa aatcgaaact ggcatctacg
     3781 ctggcggatt catcatttaa cgccacaaaa ctgtgccagc cgtcgttacc catttcgaag
     3841 cgtacgccag caaaagaagg cattaaccgc tggtagccaa aacccagcaa cctggcgctg
     3901 accacgatgc gcaggcgtac tggcgttttt tcggctgtac gctcttccag gccaaagctg
     3961 gcttgccagc gttttttacc gctcaacaaa tcacttgcga tcgccgtggc ttcggtatcc
     4021 tgctgatcat ctttaaacaa catatccgta ctggaaaaca ccagcatctt gtcctgatat
     4081 gtcgcgaaca tcagggcgtt attgccgtta tagcgcaact gataaacagg aatagtttca
     4141 ctatttatct tgatgctact gatttccgtt ttgcttaact agctgtcgct ggtagcggca
     4201 aacaacaatg gctccagcag tttgcttaaa ccactgcgct ggatcagcac catgtaatgt
     4261 gaaagatggc cttgtttatc gtgccatagc gccgcctgcg cgggctgatc taagagtgac
     4321 gaaaagagct tatctttcag cgtgagatcg tgttcataga caatgcggcg aatgctgcct
     4381 tcaatgccca gacgatcggc atgattctga taatagaaaa cgaaatcttc gctcagaaca
     4441 tcgtggagaa acggaatggt gaggagatct ttgggaagct ggctcagaga gtcgctgtct
     4501 agaaagaggt ccggctcatt gagatcgatt tgcagattgt tgtgcaccac cagcggcgac
     4561 aacgtttttt ctggcccact gccagcatat tgcaacgccc agacgccagc ggaaagcagt
     4621 gctattgcgc caaaacctac aagaccatag aaccgccagc ctttcgcctt tttttcacca
     4681 ctcattgcca cattccttgt gtatagccag ccatttttta cgggcacagc caaactttac
     4741 cgcgccctaa tacgacaaaa gcccagactt tgcagcctgg acttttcaat tcaaacaagg
     4801 gagatagctc ccttttggca tgaagaagta aaattattct tcttctggct cgtcgtcaac
     4861 gtccacttcc ggagcaattt catcatcccc ttccgcggca ctgccgtcga tggtatccag
     4921 atcttcctcg tcaaccggtt cagcaacacg ctgcagaccc actacgtttt catcttccgc
     4981 agtacggatg aggatcacgc cctgggtgtt acggcccacg atgctgattt ctgaaacgcg
     5041 agtacgtacc agcgtaccgg catcggtgat catcatgatc tggtcgcagt catctacctg
     5101 caccgcgcca accactaaac cgttacgttc ggtaaccttg atggagataa cccctttcgt
     5161 cgcacgcgac ttggttgggt attccgccac tgcagtacgt ttaccgtaac cgttttgcgt
     5221 tgcggtgagg attgcgccat cgccacgagg cacgatcaga gagacgactt tatcgccttc
     5281 acctaagcga ataccgcgca caccagtggt attgcagccc atcgcacgga cagaagactc
     5341 tttaaagcgc accactttac cttcggcgga gaacagcatt acttcatctt cgccactggt
     5401 cagatcaacg ccgatcagct catcgccgtc aaccagtttg atcgccactt taccggcggt
     5461 acgcagacgg ttgaattcgg tgaggacggt tttcttcaca gtaccgttag cggtcgccat
     5521 gaagactttc acgccttctt caaactcggt caccggcagg atcgcggtga tacgttcgtc
     5581 ctgttccagc ggtagcaggt tgacgatcgg acgaccgcgc gcgccacgag tggcttccgg
     5641 caactggtaa actttcatcg aatagacgcg accacggctg gagaagcaca gaatatggtc
     5701 gtgcgtgttc gccaccagca gtcggtcgat aaagtcttct tctttaatac gtgcggcaga
     5761 tttacctttc ccgccacgac gctgcgcttc gtattcagaa agcggctgat atttaacgta
     5821 gccctggtga gagagcgtca cgaccacatc ttcctgggtg atcagatctt ccaggttgat
     5881 gtctgcgctg ttggcggtga tttcagtacg acgtttgtca ccgaactgtt cacgaaccag
     5941 ctccagctct tcgcggatca cttccatcag acgatcggcg ctaccaagaa tacgcaacag
     6001 ttccgcgatc tgatccagca gctctttgta ttcgtcgagc agtttttcgt gctcaaggcc
     6061 ggtcagtttc tgcaaacgca gatccagaat cgcctgagct tgctgttcgg tcaggtagta
     6121 cagaccatca cgcacgccga actcaggctc cagccattcc ggacgcgcag catcgtcgcc
     6181 agcacgttcc aacatggcgg caacgttgcc cagctgccac ggattagcaa ccagcgcagt
     6241 tttcgcttct gcaggcgtcg gcgcatgacg gatcagttcg atgatcgggt caatgttcgc
     6301 cagcgccacg gctaatgctt caaggatatg agcacgatcg cgagctttac gcagttcgaa
     6361 aatagtacga cgggtcacca cttcacggcg gtgacgaaca aacgccgcga tgatgtcttt
     6421 caggttcatg atcttcggct gaccatggtg caatgccacc atgttgatac cgaaagaaac
     6481 ctgcaactgg gtctgggagt agaggttgtt gagcacaact tcaccgaccg catcgcgttt
     6541 cacttcaatc acgatgcgca taccgtcttt gtcagactcg tcacgcagcg cgctgatgcc
     6601 ttccacgcgt ttttctttta ccagttccgc aatcttctcg atcaggcgcg ctttgtttac
     6661 ctgatacgga atttcgtgga cgataatggt ttcacgtccg gttttggcgt caacttccac
     6721 ttcagcacgg gcgcggatat ataccttgcc gcgaccggta cggtaagctt cttcaatacc
     6781 gcgacgaccg ttaatgattg ccgccgtcgg gaagtccggc cccgggatgt gttccatcag
     6841 cccttcaatg ctgatgtctt catcatcgat atacgccaga caaccgttga tgacttccgt
     6901 caggttgtgc ggcgggatgt tggttgccat acctacggcg ataccggaag aaccgttcac
     6961 cagcaggtta ggaattttgg ttggcatgac gtcgggaatt ttttccgtgc cgtcatagtt
     7021 atcaacgaaa tcgaccgtct ctttttcgag atcggccatc agttcatggg caattttcgc
     7081 cagacggatt tccgtataac gcattgccgc cgcagagtcg ccgtcgatgg aaccgaagtt
     7141 accctgaccg tctaccagca tgtaacgcag cgagaatggc tgcgccatac ggacgatcgt
     7201 gtcataaacc gccgagtcac catggggatg gtatttaccg attacgtcac caacgacacg
     7261 ggcagatttt ttataggctt tgttccagtc attgcctagt acgttcatgg cgtaaagtac
     7321 gcgacggtgt accggcttca ggccatctcg gacatctggc agcgcacggc caacaatgac
     7381 cgacatcgca taatccagat aggagctctt tagctcttcc tcaatgttga ccggtgtaat
     7441 ttctctcgca aggtcgctca tctaaccgct atccctctac tgtatcccgg attcaaaggt
     7501 cgcaaattat aacacagccg cgcagtttga ggtaaaccta tacgctttat tcacatccaa
     7561 tgcctgatat actcgtttgt cttgccaatt acggagtaga agtgccaatg aatgccgaaa
     7621 aatcgccgga aaaccataac gtagaccacg aagagatcgc taaatttgaa gccgtcgcct
     7681 cccgctggtg ggatcttgaa ggtgagttca aaccgttgca ccgcattaac ccgctgcgtc
     7741 tgggctatat tgccgagcgt gctggcggtt tatttggcaa aaaggtgctc gatgtcggtt
     7801 gtggcggcgg cattctggcc gagagtatgg cgcgcgaagg cgcgacggtg accggtctgg
     7861 atatgggctt tgagccattg caggtggcaa agctgcacgc gctggaaagc ggcattcagg
     7921 tggattacgt gcaggaaacc gtggaagagc acgcggcaaa acatgccggg cagtatgatg
     7981 tggtgacctg catggagatg ctggagcacg tacccgatcc gcagtcagtg gtcagagcct
     8041 gtgcgcaact ggtgaaacca ggcggcgatg tctttttctc gacgcttaac cgcaacggca
     8101 agtcatggct gatggcggtg gttggcgcgg aatatatttt gcgcatggtg cccaaaggta
     8161 cgcatgatgt gaagaagttt attaaaccgg cagagctgct tggctgggtg gatcagacca
     8221 gtttaaaaga gcgacatata accgggctac attacaaccc gatcactaat tcctttaagc
     8281 tcggccccgg cgtggatgtg aactatatgc tgcacacgca gaataagtga ggttgatgtt
     8341 tggccgcgcc aatgcctgat gcgacgcttg cagcgtctta tcaggcctac aaatgctctc
     8401 cgtaggccag ataaggcgtt tacgccgcat ccggcaattg tgatggtcag acagcgatta
     8461 ccatttcacc gtcatcgaca aaaaccctgc cgtctgggca aaatcatcac tccctttctg
     8521 ccacgccacg ctgccgcgca gggacactcg ctgactgata ttgcccgtga ctcccacttt
     8581 tatttcaccc cgtagcttca ccgcatcgtc actgatagtg ctgccatctt cttcaatttc
     8641 cgtcgcatgg ggatcgtgat agtaattcag atctaatgtt ggtgtgacac caaccgcggt
     8701 acgccattcg ctgtgtaaac ccagccgcgt ctgaatatca tccccctgcg attgtgacac
     8761 gcgcgcatgg ttagcggcgg taaaatcatc ctgctgcacg ccctgataaa tcacctgcgc
     8821 ctgcggttca atcaccacac cacgccccgg taaccactga tacccgactt ccagcgaggc
     8881 gataatcccc gacgaatggt aacgatccac gccatcttca tgttcggaaa catcattgtt
     8941 aaaccacgcg tattgcagcc agctatccag ccaggccccc tgcttctgat taccgtgctg
     9001 ataccagctt gaggtcagcc caacggcata accgtggttc tggttatcgg cgcgagttcc
     9061 ggtcatattc gagcggctgt cgccctgatt atcactgtag ccaccaaccg ccccaagcat
     9121 ccactcgcca tccgtgcccc agcgcccgct aaacaagccg ccgctaagct gcaccgtaga
     9181 agtgtcttcc tgctgagcca gttgccctgc cgccgtgtaa tgataacgtc cgccgataac
     9241 acgtaaattc agcgtctgac catcgccacc agcgtgatcg cgtcgctcca tcataaacgc
     9301 ctgatttgcc gcccgcaggt tattgagata accgccaact ttggcattca gcaccggctg
     9361 gtaagcaggc gtaggggccg ggtcgggggt tgggtcagga tccggcgtgg gatcaggatc
     9421 gggagtcggg tctggctcag gtggcgatgg cggcgttact tcttgcgatc gcagatacca
     9481 gtcgttgtta tcttccacta gcgtgtagtc atacgctccc atgttgacgt agccactgcc
     9541 agccagactg aactgtgcat tgttttgaaa ctgcgtggga tcagctgcga aatcaaccac
     9601 tttaatgcct gtcgatgtcg gctcaccaat ccctgtaatg gagttaacca ccacagttgt
     9661 gttgccagca gtattaccgt tcatcaccaa ttgatcgctt accgagtcat cgccgtttaa
     9721 ttcgctgtcg aatagtagcg taccgccgcc ggtataatca ccgtttaccg tcagcgtatc
     9781 gccagcgacg ccattttgca ggctaaccgc accggacgta ttggtcagat caccattaac
     9841 cgtcacgtca gcaagatagg tcaggctgtc agtttcaagg ttggtattaa gctcgcctgt
     9901 caacgtcacg ccatcggtag catttaccag cgtcccatcg ccgttaaata gtgaggcatt
     9961 aagggcgacg gaagtatcct gcccggtcag acgcaaaacc gtaccatcgc tgatgtcgat
    10021 agtgccgctg gaagtagcat caattgactg aatatcctga tccgcgccag taacgaacgt
    10081 tgccccatca ccaacccaca gtgacgaagc ataaggaaag atatccgcaa catcgccttt
    10141 cagggtgcct tcttcgacac gtaccgccga ctgcgttgta ccgctggcgg tcagctccag
    10201 cgtacccgcc cccgttttag taaatgtgct accctcatcc tgatgctgcc cgctgctgtc
    10261 agccatcagt gcgccccact gcgtgtctac ccccgcatca actgccactt caccgtcggc
    10321 gcgcatttca atatcacgcc catgctcagt cgtcgtagag cgagtatcga cattggcagt
    10381 aatctccatc acgctttctt gttacttatc ggtaaagata acctggcggt tatagtgggt
    10441 atctccaagc tgcgagttgt cgctcaccat cagcgtaccg gaggcgattt gtgttgtgcc
    10501 caaataacta ttattgtttg ccagacttac ctgcccgtcg ctcgcatcga ccaccacgta
    10561 gacgccatct ttcccgtcgc cgccgatatg caaattgtcg ccacctgcaa gatctaccac
    10621 atcctgactg ccgataaccg ttccgctgct gccactgact tcaaggccat cattgtatga
    10681 tgtgccgctc tgccaagtgg agaaatcaga gagatcgagc acaccgccgt ttaacacgat
    10741 cgactgcgga tcgtcctgga gagcggtaag atctgccgcg tcgccttcca gcgaaagcac
    10801 cgcaccatca tcaacgacta tatcgccggt tagcgccatc gactgcgccc ctgccagcac
    10861 gtagctgccg ttttgcgcaa tggtgagctg accagcacct tcgatgatgc cagcaaaact
    10921 gccctgatta acagtaacgt tgccaccagc atcgatattt aaagtgccat tctgaaagcc
    10981 cgtcaatgcg tgcacaaaag tttgttgggt cgagccaacg tttagctcag cctgattctg
    11041 atactgatca atactcccta tcgtcagacc gtagcagtct tgcggatcgt cctggcaatg
    11101 cgtatcgccg acattcatca gggagttgct gcgacccagg gtaacttcac cgttttcaat
    11161 ctgcatctca ccagtaaagt cattgttatc tgcattaagt accagatcgc cggaacctgt
    11221 tttggtgatt aacccggtac cagcaataga gtcaacagct ccgtcattct ctgtattgcc
    11281 gataaccagc gtttttccgt cggcaatatc aaaggtaact tcacttaagc cgagatacat
    11341 aaagccaccc gccgcagtgg agggaccatc tccatagcct gctgcgctat tgttctcatc
    11401 gactaacacg ccgccgttct ggctgtagct gtcatcaaca gaaatatcaa taagataggg
    11461 agccgtcgcg ctattggtat atatcgcccc gccataacct tcggctgtgt tatttgtaaa
    11521 ggcggtgtta tttattatcg tataacctga aggatgcttg ctgtcgctat tattatcggt
    11581 aacatcgatt gccccgccat cgccatcact gtaacttgtt gatgtatatg cctggttatt
    11641 gttaaaaaca tcatcactta aatagacgtc attattaatg gtataaattg cgccaccttt
    11701 gccatcatta gcgatattat tgatggcgtt agtgacacgt aaatcgacgg caccagtatc
    11761 attagtacca gaagaataga ttgcgccacc atagccgcct gcgacgttac cggaaaaaat
    11821 aacatcagta agattcagcg ttgagttttc tttagcaaat atggccccgc cgttattata
    11881 ttctcctgta acggtgttat tggcaaacag agtcattcca gtttcatttt ctggtaatag
    11941 tgaaaattcc gctccttgtt gcaaaaatac ggccccaccg ctggcattat tggtcatatc
    12001 cgaaaaaacc agccattgcc catcggc
//
LOCUS       DAJTNV010000109        11802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNV010000109 DAJTNV010000000
VERSION     DAJTNV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            28..417
                     /locus_tag="OO199_003319"
     CDS             28..417
                     /locus_tag="OO199_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5537245.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            482..1780
                     /gene="tilS"
                     /locus_tag="OO199_003320"
     CDS             482..1780
                     /gene="tilS"
                     /locus_tag="OO199_003320"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5537246.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(1829..2089)
                     /gene="rof"
                     /locus_tag="OO199_003321"
     CDS             complement(1829..2089)
                     /gene="rof"
                     /locus_tag="OO199_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5537247.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(2076..2276)
                     /locus_tag="OO199_003322"
     CDS             complement(2076..2276)
                     /locus_tag="OO199_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5537248.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            2442..2987
                     /locus_tag="OO199_003323"
     CDS             2442..2987
                     /locus_tag="OO199_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5537249.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            2984..3406
                     /gene="arfB"
                     /locus_tag="OO199_003324"
     CDS             2984..3406
                     /gene="arfB"
                     /locus_tag="OO199_003324"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5537250.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            3420..4130
                     /gene="nlpE"
                     /locus_tag="OO199_003325"
                     /gene_synonym="cutF"
     CDS             3420..4130
                     /gene="nlpE"
                     /locus_tag="OO199_003325"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5537251.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(4285..5109)
                     /locus_tag="OO199_003326"
     CDS             complement(4285..5109)
                     /locus_tag="OO199_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5537252.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(5162..6880)
                     /gene="proS"
                     /locus_tag="OO199_003327"
     CDS             complement(5162..6880)
                     /gene="proS"
                     /locus_tag="OO199_003327"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5537253.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(6991..7698)
                     /gene="tsaA"
                     /locus_tag="OO199_003328"
     CDS             complement(6991..7698)
                     /gene="tsaA"
                     /locus_tag="OO199_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5537254.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(7695..8099)
                     /gene="rcsF"
                     /locus_tag="OO199_003329"
     CDS             complement(7695..8099)
                     /gene="rcsF"
                     /locus_tag="OO199_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5537255.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(8217..9032)
                     /gene="metQ"
                     /locus_tag="OO199_003330"
     CDS             complement(8217..9032)
                     /gene="metQ"
                     /locus_tag="OO199_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5537256.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(9072..9725)
                     /gene="metI"
                     /locus_tag="OO199_003331"
     CDS             complement(9072..9725)
                     /gene="metI"
                     /locus_tag="OO199_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5537257.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(9718..10749)
                     /gene="metN"
                     /locus_tag="OO199_003332"
     CDS             complement(9718..10749)
                     /gene="metN"
                     /locus_tag="OO199_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5537258.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            10937..11512
                     /gene="gmhB"
                     /locus_tag="OO199_003333"
     CDS             10937..11512
                     /gene="gmhB"
                     /locus_tag="OO199_003333"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5537259.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         2873 a         3011 c         3105 g         2813 t
ORIGIN      
        1 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
       61 attattgcga cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg
      121 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
      181 gggcaatatg tgattgagct tttctctttt ccgttcccgc cggagcgccc cagccgaccg
      241 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg
      301 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa
      361 cgcttcacct tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
      421 ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat
      481 catgacactc acgctcaata gacaacttct cacctcacgc cagattctgg tggcctttag
      541 cggcgggctt gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc
      601 gggcgtcact ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg
      661 ggttacgcat tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt
      721 acaacttgcg caagaggggc tggggattga ggcccaggcg cggcaggcac gttatcaggc
      781 atttgcccgt accctattgc ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca
      841 atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
      901 ggcggaagtt tcggagtttg ctggaacacg gcttatccgc ccgttgcttg cccgcacgcg
      961 gggggaactg gagcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa
     1021 tcaggacgac tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca
     1081 gcgttggccg cattttgcgg aagcaacggc ccgcagcgcc acactttgtg ctgaacaaga
     1141 gagtctgttg gatgaactgc tggcggatga tttagcacac tgccaaacgt cgcaggggac
     1201 gctgcagatt gcgccaatgc tggcgatgag tgatgcccgt cgcgcggcga ttattcgccg
     1261 ctggctggca gggcagaatg caccgatgcc ttcccgcgac gcgctggtgc gcatctggca
     1321 ggaagtggcg ctggcgcggg aagatgcttc cccctgttta cgtttgggcg cctttgaaat
     1381 ccgccgctat cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gcgaaaccat
     1441 tgtgctgtgg cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaaccgtaca
     1501 gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
     1561 caaagcgcca ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat
     1621 ctggcaagag ttaggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg
     1681 agaaacgctg attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga
     1741 gaatggcgta agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc
     1801 atcaaacgtc gcatccggcg aaagtcaatc aggattcgct taccaccacc gtaccgattt
     1861 cagggtggct aaagctggta attttatcca gacgcagctc gcgggtttcg ccagcggcct
     1921 cgacgaccag atactccaca tttttgcggg agactaaatc actggctttt gcctgcaatt
     1981 tttcgccatc tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat
     2041 tatcgtaatc atcacaatta attggttgat acgtatcatt cattgacata atcgctcacc
     2101 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
     2161 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
     2221 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
     2281 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
     2341 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
     2401 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
     2461 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc
     2521 tgacgctggc gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt
     2581 ggctgaaata tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc
     2641 cggaagcgtg gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc
     2701 cggatgagcg acgaattaag aaagcctgca cccaggccgc agaagtggcg ctgtttacct
     2761 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg
     2821 ccaatctttc cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg
     2881 atcgtaccat gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata
     2941 agaataatct ggaagttaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg
     3001 acatgttgct attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgctgg
     3061 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc
     3121 cagcctgcca gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag
     3181 tgatggcgtg attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc
     3241 cgctctggcc cggctggtgg cagtgattaa agatttaaca aaagaacaaa aagcccgacg
     3301 acccacgcgg cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc
     3361 aagcgtgaag gcgatgcgcg gcaaagtacg cagcggtcgg gaataaaaag aaggaatgga
     3421 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat
     3481 gtaataatcg ggccgaagtc gatacgcttt ctccggcgca ggctgccgaa ctgaaaccga
     3541 tgccgcaaag ttggcgcggc gtgctaccgt gtgccgattg cgaaggaatc gaaatctctc
     3601 tgttcctcga aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag
     3661 aaccttcctc cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa
     3721 ccgacagcaa aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg
     3781 atcgtgaagg caatccaatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca
     3841 gtttacctat gacgccgatg accctgcgag gcatgtattt ttatatggct gatgcggcga
     3901 ccttcactga ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc
     3961 gtagctacct ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc
     4021 actttacgct tgaggctaat ccggatactg gtgcgccaac caaagtatta gcgcccgata
     4081 cggcgggtaa attttacccg aataaggatt gcagtagttt ggggcagtaa acctgcttaa
     4141 ggcagaaaca aacgcaaaat tgcctgatgc gctgcgctta tcaggcctac agcatataat
     4201 gcaacatttt gaattagcac gattttgtag gccggataag gcgtttacgc cgcatccggc
     4261 attttgtgca cgaggctggc tttatcactg agtcagccca acaaagcgac ccgccttgat
     4321 ataaatccca ggcttcaggt gcccaacata ctgtaactgc gtttctggct taaacgcaga
     4381 gacatcacca atacgcatat gcatcagatc ggcagggcta atccagcccg attgcgccag
     4441 tgtaagtgga tgcccggatt tggcaaatgc atcagtgaca aattccgaac aaaaccacga
     4501 ctttttgtct ccttcgcaca cactgcttaa ctgcgctttc gccaggtcgc tgacgcactg
     4561 ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca ccataaaggg
     4621 aataaattcg acaatgccgc gatagttata accgctatct ttgattttat tggcaaacgc
     4681 ggtgatatct gtggcttgtt gcggggtaag atccgggact cgtaagacga aaagcttatc
     4741 actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg tcgcttctgc
     4801 aacgttatta tcacccagaa agattgcaac gtgactcaca gaggaagtgc tgaagacgcg
     4861 gattccaaat gaggttaccc caaggcttga ggagaacagc aaatcgccgg gtttgagatc
     4921 aggtgccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct ggaatttgac
     4981 agcccatgtt tttgcctcgg catctactgc tgtggctgat gggtctggct ggctgatatc
     5041 aacggtacaa gccgaaagta aaagaaagct ggggagaagc aggcggcagt acgcctttgg
     5101 tttatccatt ttatacaatc catgtaaaaa agggccctga aattcaggac cctttctggc
     5161 atcagccttt aatctgtttc accagatatt cgacgatgtc accagtctta attaactgtt
     5221 tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca
     5281 gcacaatagt gtgcggaata ccgatcagtt ccatatcagc aaacatcacg cccggacgct
     5341 ctttgcggtc atccagcagc acttcgatac cttgtgcacg cagttcgctg tacagtttct
     5401 cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat cggcagaatc gccacctgga
     5461 acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg
     5521 cagctaccac acgcgttacc ccgataccgt agcaacccat cgtcaggatt tggttacggc
     5581 catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc agctggaaga
     5641 tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc ctggccatcc gggcttggat
     5701 cgccagccac cacgttacgg atatctgcaa cttccggggt agcgacatcg cgatcccagt
     5761 tgataccgaa gtagtgttta ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg
     5821 caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt gaacccggac
     5881 cggctttaac cacagcacga atttcttctt cggtcgcgaa agtcagcggg cttgcaacct
     5941 gcggcagttt ttctgcttta acttcgttca gctcgtgatc accgcgcacc agcagcgcaa
     6001 ccagcgggaa gctactgcct tcaaccgctt taaccagcag agtcttaacc gttttctcaa
     6061 tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc gtatcaacca
     6121 gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt
     6181 caatgttcgc tgcatagtca gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg
     6241 ccagcacctg gaattcgtga gaggcgctgc cgccgataga accggtgtcg gcctgtacgg
     6301 cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac attgcatcgt
     6361 aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc atcaggaatt
     6421 cgcgggaacg catgacgccg aaacgcggac gcacttcgtc gcggaacttg gtctggatct
     6481 gatagaagtt cagcggcagc tgtttgtaag agctaagctc gttacgaatc aggtcagtga
     6541 taacttcttc atgagttggg ccgagtacga acggacgctc gccacggtca acaaaacgca
     6601 gcagttccgg accgtactgt tcccaacgac cactctcttg ccacaaatcg gctggctgaa
     6661 ccaccggcat cgacacctcg atcgcaccgg cgttgttcat ctcttcacgc acgatgtttt
     6721 cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg gaggccagct
     6781 tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg tcggcaggtg
     6841 tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg ttccagttgg
     6901 aaggtagaac aggctcaagg cgagcctggg acaaaaaaag tgatttagtt taccagtgca
     6961 aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa agacttcaaa
     7021 acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt agccagacgg cataggtttt
     7081 gcccgtttcc tcacctttac gataggccgg gcgcgggtcc tgcgccagta cttcgcggat
     7141 aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa agctgctttt cgacctctgc
     7201 ggtaaaactc accgccatct ctgcagctgg cgcactttgc gcatagctgg cactggcatc
     7261 gggaagcgat tcggcaaagg ggagatacgg tttgatatcc actaccggcg taccatcgac
     7321 cagatccaga ctgccgagct tcagaatcac gctgccttta tggcaaacaa cctctttcag
     7381 ctctaccagc gacatgccaa ttgggttagg gcggaaagta gagcgtgttg cgaaaacccc
     7441 cattctggcg ttaccgccga ggcgcggtgg acgcacagtc ggacgccagc cgccttccat
     7501 cgtttgatga aagacgaaaa ggatccataa atggctgaat gcttccaggc cgcgaacggc
     7561 gtcggcctgg ttgtaggaag caatgagatg cagttcgccg ttggcgcttt ttaccagacc
     7621 tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacgc ctatttgctc
     7681 gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag agccgataca tacagcctga
     7741 cgatagcagc ccggcgtacc gctggtgact tcgcagctat gcagtaatac agcattggct
     7801 ttcattttag aggcattgat ttgcatctgc ttacgtgcgg ttggaatgct cggcggagag
     7861 tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg gaacggtttg
     7921 ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg cgctttcggt
     7981 tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac aggggatctg
     8041 cttaacatgg aacagccgct tagcatgagt gctactaaac agatcggtaa agcacgcata
     8101 gtatttcctc aatgtatgat caaaacgtca atattgaatc aggagcttgt aaaaatgaca
     8161 agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc taaaaattac
     8221 cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac ttcatcagac
     8281 tgataagcct ggacgaattt cttcacgttc tcggcgtctt tgttatcttc acgcgttacg
     8341 atcaggttta cgtatgggga gtctttatct tcgacaaaga taccgtcttt cgccggagtc
     8401 aggccaatct ggctggcata ggtggtattg ataactgcca gagcgatttg cgcgtcgtcc
     8461 agagaacgcg gcagttgcgg tgcttccagt tcaacaattt tcagattttt tgggttctca
     8521 acaacatcaa gaacggtcgg cagcaggcca acgccatctt tcagtttgat caagcccact
     8581 ttttgcagca gcagcagtga acgaccaaga ttagttggat cgtttggcac ggcaacctgc
     8641 gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc aatcggataa
     8701 acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg ctgatcaagg
     8761 tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc ttcgtttggc
     8821 agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc tttcgcaact
     8881 ttctgcgcga cttctgctac ctgctgttcg gcaccaacaa tcacgccgac tttaatgtgg
     8941 tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat cagggctccc
     9001 actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa ttatttatgt
     9061 tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc gaactgaatt
     9121 aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc gttgtagccg
     9181 atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc accacccatc
     9241 gcggaataac cgaccagggt aatcagggtg atagttgccg cattcaccag acccggcaac
     9301 gcttccggta acagcacttt acagacgatc tgcattggcg tggcacccat tgcgcgggaa
     9361 gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg ggcaataaac
     9421 ggtgctgcac caacggttaa cggaacaatt gctgcctgca aaccaatcga tgtaccgaca
     9481 ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat ggaacggaaa
     9541 atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt tagcaataat ttgccccgga
     9601 cgcgtgacat aaagcagaac gccaaccggc agaccaatca caaacccaaa aaatccggat
     9661 acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat catcggctca
     9721 gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa tggcggcttg
     9781 cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaatttaa cgccaccggc
     9841 gtagtccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg cggtttcaga
     9901 aagcagtggg gcatcgaccg attgaccggt aaactccaga cgcagcatcg gcacgcagtc
     9961 agtaaatggc tccgtttgca gacgttcctg gtaatcttcc gggatatcca gatgcagggt
    10021 cgactgaata aacttctgcg ccagcggcgt tttcggatgc gagaacactt cacttaccgt
    10081 gtcctgctcg atcagttcgc cattgctgat gaccgccacg caatcacaaa tacgcttcac
    10141 aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt taatgtcttt
    10201 cagcagttcg agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg cttcatcaca
    10261 cagcaatact ttgggattgc tggctaatgc acgggcaatc gccacacgtt gtttctgccc
    10321 accggaaaga ttcgacgggt agctatcatg cttatcgcca agaccaacca atgacagcaa
    10381 ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg tccagctcca gcggcagagc
    10441 cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa tcataccaat
    10501 ctggcggcga gctttggtca actcggattc tgacagcgtg gtcaattcct ggccatcgac
    10561 cagcacgcta ccctcggttg ggcgctccag caggtttaca caacgtataa gcgtactctt
    10621 acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat gcaggctgac
    10681 gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga tattcgaaag
    10741 ttttatcatt gattatttat tatcgtcatt aagttagtcg tagcatctcg aatgcctgaa
    10801 acgggcaacg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt
    10861 ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtcc catgccttat
    10921 taaggagcta taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc gtgatggcac
    10981 cattaatgtc gatcacggct atgtccatga gatcgacaac tttgaattta tcgacggtgt
    11041 tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag taaccaacca
    11101 gtctggcatt gctcgcggta aatttaccga agcacagttt gaaacgctga ccgagtggat
    11161 ggactggtcg ctggcggacc gagatgtcga tctggatggt atctattatt gcccgcatca
    11221 tccgcagggg agtgttgaag agtttcgcca ggtctgcgat tgccgcaaac cacatccggg
    11281 gatgcttttg tcagcacgcg attatttgca tattgatatg gccgcttctt atatggtggg
    11341 cgataaatta gaagatatgc aggcagcggt tgcggcgaac gtgggaacaa aagtgctggt
    11401 gcgtacgggt aaacctatta cacctgaagc agaaaacgcg gcagattggg tgttaaatag
    11461 cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa ccggcgcaat gattaaaaga
    11521 tgaacggttg aaataaaaat gcatttttcc gcttgtcttc ctgagccgac tccctataat
    11581 gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacagccggt tcggttgaag
    11641 agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagtgtgata tacgccacct
    11701 cgcgacagtg agctgaaagc tgcgtcgcaa ctgctcttta acaatttatc agacaatctg
    11761 tgtgggcact cgaagatacg gattcttaac gtcgcaagac ga
//
LOCUS       DAJTNV010000110        11694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNV010000110 DAJTNV010000000
VERSION     DAJTNV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            116..985
                     /locus_tag="OO199_003334"
     CDS             116..985
                     /locus_tag="OO199_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5537260.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1188..1541)
                     /locus_tag="OO199_003335"
     CDS             complement(1188..1541)
                     /locus_tag="OO199_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5537261.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO199_003336"
     CDS             complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO199_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5537262.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3369..3662)
                     /locus_tag="OO199_003337"
     CDS             complement(3369..3662)
                     /locus_tag="OO199_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5537263.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3938..5194
                     /gene="yjeH"
                     /locus_tag="OO199_003338"
     CDS             3938..5194
                     /gene="yjeH"
                     /locus_tag="OO199_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5537264.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO199_003339"
     CDS             complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO199_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5537265.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6023..7459
                     /gene="aspA"
                     /locus_tag="OO199_003340"
     CDS             6023..7459
                     /gene="aspA"
                     /locus_tag="OO199_003340"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5537266.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7577..8878
                     /gene="dcuA"
                     /locus_tag="OO199_003341"
     CDS             7577..8878
                     /gene="dcuA"
                     /locus_tag="OO199_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5537267.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8994..9332
                     /gene="cutA"
                     /locus_tag="OO199_003342"
     CDS             8994..9332
                     /gene="cutA"
                     /locus_tag="OO199_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5537268.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9308..11005
                     /gene="dsbD"
                     /locus_tag="OO199_003343"
     CDS             9308..11005
                     /gene="dsbD"
                     /locus_tag="OO199_003343"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5537269.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGSKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11042..11617
                     /locus_tag="OO199_003344"
     CDS             11042..11617
                     /locus_tag="OO199_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5537270.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2810 a         3022 c         2877 g         2985 t
ORIGIN      
        1 atagagtgag cacggctatt tatgttattt taacaactat tactaccaag cccatttatc
       61 tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa cctacatggc
      121 cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact
      181 ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga
      241 actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata aactggatgc
      301 tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa tggcagaaaa
      361 catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac
      421 gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg
      481 tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc gagcaatcat
      541 tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct cactgttaga
      601 tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat atgacaccta
      661 ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc tttaccgctt
      721 taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac
      781 gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa
      841 aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca
      901 accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac aatccaccag
      961 caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca
     1021 gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc
     1081 cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg
     1141 aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca gttcgggcac
     1201 ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc
     1261 gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga
     1321 tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg
     1381 ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca
     1441 atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt
     1501 agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat
     1561 gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga tgaccaaaag
     1621 aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg cagggcaatt
     1681 acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg
     1741 catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg ccagccacag
     1801 aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga
     1861 tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa
     1921 cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag
     1981 cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca
     2041 ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt
     2101 cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt ttctctttca
     2161 tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta
     2221 cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga
     2281 tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg
     2341 tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca
     2401 gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca
     2461 gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc
     2521 cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct tcagcgatga
     2581 tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt
     2641 tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt
     2701 ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca
     2761 cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga
     2821 ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg
     2881 taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct ttcagttctt
     2941 caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg
     3001 cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg
     3061 taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca
     3121 tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga
     3181 tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga ccgagggtaa
     3241 ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac
     3301 cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg
     3361 tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct
     3421 tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca
     3481 actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc
     3541 agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca
     3601 gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc
     3661 attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg
     3721 gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa
     3781 gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat
     3841 cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc
     3901 agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact
     3961 ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc
     4021 cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt ggcccgtttt
     4081 gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag
     4141 cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac
     4201 cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac aaattgccgc
     4261 cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg
     4321 tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg ctaatctaca
     4381 aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat
     4441 caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc
     4501 tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc
     4561 ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc tgctgctggc
     4621 aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca
     4681 aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt
     4741 atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca tttatataca
     4801 gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg
     4861 cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt
     4921 gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta tttatgccaa
     4981 tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg
     5041 tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt
     5101 cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt ttctgccaaa
     5161 acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc
     5221 gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac tcaccatcaa
     5281 aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca
     5341 aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac
     5401 cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata atcagcgaaa
     5461 cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc tgcatcagca
     5521 cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa
     5581 ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct
     5641 caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta
     5701 atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca gcttatattg
     5761 tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg
     5821 gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga
     5881 ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa
     5941 tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg
     6001 cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg
     6061 taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa
     6121 cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat
     6181 ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta aaagtgtagc
     6241 gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt
     6301 cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt
     6361 gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa
     6421 cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg
     6481 tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg
     6541 ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca
     6601 ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga
     6661 agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac
     6721 agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact
     6781 ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga
     6841 ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa
     6901 aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa
     6961 cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc
     7021 ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc
     7081 agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc aggccatgtt
     7141 cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg
     7201 catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac
     7261 ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga
     7321 aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact
     7381 tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac
     7441 tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac
     7501 gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa
     7561 aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg cgatcttctt
     7621 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
     7681 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
     7741 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
     7801 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
     7861 ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
     7921 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta ctgcagtggt
     7981 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
     8041 cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
     8101 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
     8161 tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc gcggtgaaaa
     8221 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
     8281 tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
     8341 gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt
     8401 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
     8461 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
     8521 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
     8581 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
     8641 gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt
     8701 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
     8761 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
     8821 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
     8881 atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt
     8941 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
     9001 atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga
     9061 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
     9121 tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
     9181 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
     9241 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
     9301 acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca cttccgtttt
     9361 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc
     9421 ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta
     9481 ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgagca
     9541 gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
     9601 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
     9661 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
     9721 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca
     9781 gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
     9841 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtagtaa
     9901 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
     9961 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
    10021 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
    10081 aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat
    10141 gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg cgattgccgg
    10201 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca
    10261 aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct
    10321 gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat
    10381 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
    10441 gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt
    10501 tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat
    10561 aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
    10621 gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa cggtagatga
    10681 gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga
    10741 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
    10801 agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt
    10861 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
    10921 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
    10981 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
    11041 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
    11101 caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg
    11161 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
    11221 agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
    11281 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt
    11341 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
    11401 gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg aagttgacga
    11461 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
    11521 ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg
    11581 cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat
    11641 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg aaat
//
LOCUS       DAJTNV010000111        11670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNV010000111 DAJTNV010000000
VERSION     DAJTNV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            597..794
                     /locus_tag="OO199_003345"
     CDS             597..794
                     /locus_tag="OO199_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537271.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO199_003346"
     CDS             complement(744..1937)
                     /locus_tag="OO199_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537272.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO199_003347"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO199_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5537273.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO199_003348"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO199_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5537274.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO199_003349"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO199_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5537275.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO199_003350"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO199_003350"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5537276.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO199_003351"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO199_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5537277.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO199_003352"
     CDS             complement(10573..10716)
                     /locus_tag="OO199_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537278.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11670
                     /locus_tag="OO199_003353"
     CDS             10966..>11670
                     /locus_tag="OO199_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5537279.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2962 a         2900 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggactca
//
LOCUS       DAJTNV010000112        11631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNV010000112 DAJTNV010000000
VERSION     DAJTNV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(282..357)
                     /locus_tag="OO199_003354"
     tRNA            complement(282..357)
                     /locus_tag="OO199_003354"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO199_003355"
     CDS             916..3168
                     /locus_tag="OO199_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537280.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="OO199_003356"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="OO199_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5537281.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="OO199_003357"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="OO199_003357"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5537282.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="OO199_003358"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="OO199_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="OO199_003359"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="OO199_003359"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR5537283.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="OO199_003360"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="OO199_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5537284.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="OO199_003361"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="OO199_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5537285.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO199_003362"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO199_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5537286.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="OO199_003363"
     CDS             complement(11389..11547)
                     /locus_tag="OO199_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5537287.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2992 a         2855 c         2900 g         2884 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DAJTNV010000113        11562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNV010000113 DAJTNV010000000
VERSION     DAJTNV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            231..290
                     /gene="ibsE"
                     /locus_tag="OO199_003364"
     CDS             231..290
                     /gene="ibsE"
                     /locus_tag="OO199_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5537288.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(410..1843)
                     /gene="hldE"
                     /locus_tag="OO199_003365"
     CDS             complement(410..1843)
                     /gene="hldE"
                     /locus_tag="OO199_003365"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5537289.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1891..4731)
                     /gene="glnE"
                     /locus_tag="OO199_003366"
     CDS             complement(1891..4731)
                     /gene="glnE"
                     /locus_tag="OO199_003366"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5537290.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4754..6055)
                     /gene="ygiF"
                     /locus_tag="OO199_003367"
     CDS             complement(4754..6055)
                     /gene="ygiF"
                     /locus_tag="OO199_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5537291.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6297..6917
                     /locus_tag="OO199_003368"
     CDS             6297..6917
                     /locus_tag="OO199_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5537292.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6981..8219
                     /gene="cca"
                     /locus_tag="OO199_003369"
     CDS             6981..8219
                     /gene="cca"
                     /locus_tag="OO199_003369"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5537293.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8382..9203)
                     /gene="bacA"
                     /locus_tag="OO199_003370"
     CDS             complement(8382..9203)
                     /gene="bacA"
                     /locus_tag="OO199_003370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5537294.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9294..9662)
                     /gene="folB"
                     /locus_tag="OO199_003371"
     CDS             complement(9294..9662)
                     /gene="folB"
                     /locus_tag="OO199_003371"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5537295.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9767..10384
                     /gene="plsY"
                     /locus_tag="OO199_003372"
     CDS             9767..10384
                     /gene="plsY"
                     /locus_tag="OO199_003372"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5537296.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10397..11328)
                     /gene="ttdR"
                     /locus_tag="OO199_003373"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10397..11328)
                     /gene="ttdR"
                     /locus_tag="OO199_003373"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2721 a         3308 c         2860 g         2673 t
ORIGIN      
        1 taacacatat ccctggccca aaaagccggg gattttttat atctgcgttc cgctaaaagg
       61 tgcaaatgct caggacgttg cagcgttttg cgtgaccgct cggggaaggc aaaactgctt
      121 cagggaaagc gttgcgcggg gtccggcgct catcaacaat cggggagtca gcaaggggct
      181 gaaacgggaa agcccctccc gaggaagggg ccttatagaa ggaaagggtt atgatgaagt
      241 tcgtcatcat actggttgtg ttgttactgt taagtttccc gacttactaa caactgatca
      301 gaggggggag aaatcctccc ttacccttgt tcctttactt taggttgaaa aaacaacagc
      361 gtcaataggc ctgcctgcta cgaagcgaga tctgtgaacc gctttccggt tagccttttt
      421 tatcctgttg gatcttcttg atgatgttag tggtcgagca accgtcttcg aagttgagca
      481 ccagcacttc gccgccgttg gcccagactt ctttactccc ggcgatctct tctggtttat
      541 agtcgccgcc cttcaccagt aaatctggca ggatcccggc gatcaagcgc tgcggcgtgt
      601 cttcttcaaa cgacaccacc cagtcgaccg cttccagtgc gcccagcaca atcatacgct
      661 gttcgagcgg gttaacgggg cgagaatccc ctttcagccg tttggtggag gcatcgctgt
      721 tcacagcgac aatcaagcgg tcacccagtt tgcgggcatt tgccagatag aagacgtgtc
      781 cggcgtgcag gatgtcaaag acgccattgg tcatcaccac tttttcaccg cgtttacgcg
      841 ctgccgctac ggccagcttc agttcctctt cggtcatcac gccaaagccg gtatctgcgc
      901 gcccacgcac tgcattttcc agctcgatcg gcgaaaccgt ggaggttccc agtttgccga
      961 ccaccacgcc agccgccgca ttggcaaaga agcaggcttc ttccagcgaa ttacccgctg
     1021 ccagcgttgc cgccaggacg ccaatcaccg tgtcgcccgc accggtaacg tcatacactt
     1081 cctgcgcctg ggttggcata tgcagcggcg ctttacccgg ttgcagcagc gacataccct
     1141 gttcggaacg ggtcactaac agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc
     1201 gctcaacaat ctcttcttcg gtcttacatt taccgacaac agcttcaaat tccgagagat
     1261 tcggtgtcag cagcgtagcg ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca
     1321 gcaccggaac gcccgcttta cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac
     1381 ctttggcgta gtcagaaagc accagcgcgc caatcgaact cagcgcctga ttaatccgct
     1441 cgtgcagcgg ctgcggatca acaccttcga aaccttcttc aaaatccaga cggatcagct
     1501 gttggttgcg ggaaagtacc cgtaatttgg tgatggtcgg atgcgtcggt acagaaacga
     1561 agtcgcattt gacgttgacg tcggccagag atttactcag cgcgcgcgct gcatcgtcaa
     1621 tgcccgtcaa cccgaccagg cgtgcattag caccgagaga agcgatattc atcgccacgt
     1681 tagccgcgcc gcccggacgt tcttcgatgg tattcacttt aaccacgggc accggcgctt
     1741 ccggcgagat acgactggtg gggccgtacc agtaacgatc cagcatcaca tcaccaacca
     1801 ccatcactcc tgcacgttca aactctggca gcgttacttt cattcctgtc tcctgagaga
     1861 ttcaaaattt gcgcgcgata ataccatact tcattcttcc accagccact tctgccagct
     1921 tgcccgcacc agatcacgct ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc
     1981 ctgtaatgcc agatgatgaa gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat
     2041 cgcttcctgc tcttccataa tgtcgttttg cgccagcagc tccagaatac gcacgttgtc
     2101 tgaccagcgc gttaacttcg gtttttcatg agcgtagcgc aacaccagat attgggtaat
     2161 aaattcgata tcggtaattc cgccttcatc ggctttgata tcaaagcgat tacgatgttt
     2221 attgccgaga tgagcgcgca ttttctcgcg catttcccgc acttccgttt gcagagtttt
     2281 accttctcgc ggcagtgtca taatctcacg acgcaccgcg tcaaagtgcg cagtgagctg
     2341 cggatcgccg tacactacac gcgcacgcac cagcgcctga tgttcccacg tccaggcctc
     2401 gtttttctga taatcggcaa atgcttctgc ggatgtcacc agcattcccg ccgccccgga
     2461 cggacgcagt cgagcatcca cttcatacaa aatgccggaa gaggtacgcg tactgaacag
     2521 atgcataatg cgttgcgcca gacgcagata aaactgccgc ccgtcgattt cccgctcgcc
     2581 gtcagtcatc gcatccattg ggcaatcatg gaggaagata aggtcaagat cggaactgta
     2641 gcctaactcc cagccgccca gcttgccgta gccgaccacc gcaaaaccgc gcccttcacg
     2701 ttcgttgagg tgatttggct taccgtagcg ggccaccatt tgaccccacg cctgctgaac
     2761 gacggcatct atcatagctt ctgccagcca ggttaagtga tcgctcactt tcattaccgg
     2821 tagcgtaccg gcgatatccg ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg
     2881 cagcgcctca agctgttgtt cttcgtcatc ttccggcacg cgcagcaaat actggcgcag
     2941 ttcatcacgg taggcatcgg tcgccgtcgg ctgataaagg gtatttggat cgagcaattc
     3001 atccagcaat aatggataac gcgctagctg gctggcaatc atcggcgatg cggcacacag
     3061 agaaatcaaa tgtttaagcg ccgcagggaa ttcactgagc aattctaaat aggttgtgcg
     3121 ggtaacaatc cccaccagca aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg
     3181 cgcacagaca tcacttagca gatgtggcat cagatggtcg agcacctgac gtcctcgcgg
     3241 cccgatagta cgcttatcca gctctttgcg gaaatcagca atcagcgtta ggacctgttt
     3301 gcgatcatcc tcgctaagat gcgccagcac tggcgtagtg tcatcttcct gcaacgcatc
     3361 ctgccacagc tcacgccact gttccgacag cgactcttcc tgagtttcac tttcgtcgtc
     3421 gccaatcaat tcattaaaca cccggcgcac attggtcata tgtgcagtca gcgccccggt
     3481 cagttgcggc cagtcagcaa agtccatcgc ccacgccagc cgcgcacggt taagctcatc
     3541 agaaggaagc gtttgggttt gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg
     3601 caggaacaga tatgccactc gcaattgttc agcatcgttt tcagaaagca gatgcagcgc
     3661 ggcaatggcg ctgagcgttg gcagtaaaga gcgcgactgc agcaacggtt cgcgcccacc
     3721 gcgaatgagc tggaacacct gaacgataaa ttcaatttcg cgaatgccgc ctgcgccgag
     3781 tttgatattg tcggtcaaac cacgtcgacg cacttcacgg gcaatcatcc ctttcatgtt
     3841 gcgcagcgac tgaatcacgc tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat
     3901 cgcacgcaac tcgttagcat agacgccttc gctatcgccc ataatccgcg ctttgaccat
     3961 cgcgtaacgc tcccagtcgc gtccctgctc ctggtaataa tcctccaacg cggcaaagct
     4021 cagcaccagc gggccacttt cgccaaacgg acgcagtcgc atatccacgc gatagacgaa
     4081 gccatcctgc gttggttgat ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa
     4141 ctgcgcgtta tccagttccc ggcgtccgcc ctgcgtacaa ccatgttccg gccaggcaaa
     4201 aatcagatcg atatcagagg agaaattcag ttccccgccg cccagcttac ccatgcctaa
     4261 gatcagcagc ggttgcgctt cgccctgcgc attgcacggc gttccccact cgcggcagca
     4321 ggcgtcatac agccagtcac gcgccgcaac aatcagcgtc tccgccagat aactgagctg
     4381 ctgcaatatg ctctcttcag taaccagtgc cagcgtttgc gcccatgcga tgcgcaccat
     4441 aatgcgccgc cggaatagcc gcagctcgcg cattaacccg gcttcgtcgc tcacattact
     4501 gagcgcctcc tgcaaccaca cgctgtaatg ttgccattcg tcagcctgtg gcggttggct
     4561 ttccagttcc gtcagccact ctggatgcgc aatcacgctg tcctgcacaa aatcactgaa
     4621 agtaagtact gacttcgcct gtgcactaag tgattcctcg gctaaaagct ctggcagccg
     4681 ctcaacaacg gtctgccagt actgctgtaa cggtgaagag agcggcttca taagcgattt
     4741 tatccttgcc tgattaacgt tttccactgt gcaaccagaa cggttcctga ttgtttgcct
     4801 cattacggaa atgttcaatt tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct
     4861 gccagttctc cagccaggct tgcgcgacga caggatcata gtaacccgcc agcaacagta
     4921 ttgagtcaat atcacgcatc aggcgtggca actggtcacg gtagcgatcg cctaacggct
     4981 ggcagaaaac gcttttcagt tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt
     5041 tgaaggagtc gctaattttg ccctgcgctt tggcatctaa aaacggctgc catgctttgc
     5101 ttaccagcca ttcggtcaac gccagcttcg ccattgccgt ttcggtagag tagaccgccg
     5161 tcacggcaga aaccgcagaa gcgatggtcg cttcgcattg agtcagcaaa tcacgtaagt
     5221 gagtactcgc tttacgcggc acaatgccac cgaacagcat cagtgtatga cggactaggc
     5281 taatggctgc cagcacctgc tctttcgccg catcgttgcc gcgtacccac agttcttcat
     5341 gatactgcca ttgcgctaac accagctcga gcgccgcttc cagcccctgt tccacatcgg
     5401 cttttgccgc aacatgcaaa atggtggtcg gtttgatttc acgcgccgga ttgccctgcg
     5461 ccagatgata gccacgcgcc gctttgctca ggctgccctg gcgtaatccg gtttgcgata
     5521 ccagttggtt cgccagtttc agcaccgcgc gcgtgtcgcc gctaagcagc tccagttcca
     5581 gctcgcagat aggctcagca aattcacccg ctttcacttc cccctggtcg agagcgattt
     5641 caattcggct gccattgatc tctactagcc atttttcgcg ataaaaatcg gtgctaaaca
     5701 gcgactgcac gcgggaagcg agatcggcag gcaattcgcc gttcggccag atttccgtcg
     5761 gtaactgcgc caggtcgagc gtcggttcgc tcaacgccac gttatattcc gggcgctggt
     5821 gtaagccgcc tgtcactctt cctgcaactt tcatggtcat ctcatagcga ccgttttcgc
     5881 cacgaatacg taagcccata tcgtgcctac gcagccagtt atccggcgtt tcgtagtaaa
     5941 tattcagcaa ctgcacgggg tcatggtgct cgccgcccag cgtattgaga tggtcacgca
     6001 acgcctcaac ggcactgtga ttaacaataa actttaattc gatttcctga gccatggcct
     6061 ttacttatcg gttatgtcac ataagtgacg atgaacggcg aacttaatgc gatctttttt
     6121 gtcagtagat agtattttgc gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa
     6181 gtttgaagca gtggcaaggc ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt
     6241 agcgacactg atgccactac tatcgttcca ctttccatga caataacgac agcctgatgc
     6301 caaaattacg cctgatcgga ttaactttac tcgcacttag cgcgactgcc gtctcacacg
     6361 ctgaagaaac gcgctatgtt tctgacgaac tgaatacctg ggtccgtagc ggtccgggag
     6421 atcattatcg cctcgtgggc acggttaacg ccggcgagga agtgacctta ttacaaactg
     6481 acgccaacac caattatgcc caggtgaaag acagctctgg ccgtaccgcc tggatcccgt
     6541 tgaaacaact tagcactgag ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg
     6601 tcaaaaccct gaccgataaa ctcaccaata tcgataacac ctggaatcag cgcaccgcag
     6661 aaatgcagca aaaagtggcg cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc
     6721 agaaactgaa aaacgagctg attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc
     6781 tggatgacaa acagcgcacc atcatcatgc aatggtttat gtatggcggt ggcgtgctgg
     6841 ggcttggctt gctgctcggt ctggtactgc cgcacctgat cccaagccgc aaacgcaaag
     6901 atcgctggat gaactaaatc gccttcttcg ttgcactgtc ttattattag gaattgtgta
     6961 tttgacgaag ggatgatggc gtgaagattt atctggtcgg tggtgctgtt cgggatgcat
     7021 tgttaggact atcggtcaaa gacagagatt gggtggtggt cggcagtacg ccacaggaga
     7081 tgctcgacgc gggctaccag caggtaggcc gcgattttcc tgtgtttctg catccgcaaa
     7141 cgcatgaaga gtatgcgctg gcacgtaccg aacggaaatc cggttccggt tacaccggtt
     7201 ttacttgcta tgccgcaccg gatgtcacgc tggaagatga tcttaagcgt cgcgatctga
     7261 ccattaatgc gctggcccag gacgataacg gcgagattat cgacccgtac aacggtctgg
     7321 gcgatctgca aaatcgtctg ttgcgccatg tttcccccgc ttttggcgaa gatccgttac
     7381 gcgtactgcg cgtggcgcgt tttgctgcgc gttatgccca cctcggtttt cgtattgccg
     7441 atgaaactct gacgctgatg cgcgagatga cccatgcggg tgaactggaa cacctgacgc
     7501 ctgaacgggt atggaaagag acggaaagcg ccctcaccac ccgcaatccc caggtgttct
     7561 tccaggtact gcgcgattgc ggcgcacttc gcgttttatt cccggaaatt gacgcactgt
     7621 ttggcgttcc ggcacctgcc aggtggcatc cggaaatcga cacgggtatt cataccttaa
     7681 tgacgctctc aatggcggcg atgctgagtc cgcaggttga tgtccgtttc gcaactttat
     7741 gccacgatct cggtaaagga ctgacgccgc cagaactttg gccgcgtcat catgggcatg
     7801 gcccggcggg tgttaagtta gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa
     7861 ttcgcgattt agccagactg gtggctgagt ttcacgatct catccgcacc ttcccaatgc
     7921 tgaacccaaa aaccatcgtc aaattatttg attccatcga tgcctggcgt aaaccgcagc
     7981 gtgtcgagca actggcgctg accagcgagg ctgacgtgcg cggcagaacc ggttttgaat
     8041 cagcggacta cccgcaaggc cgttggttgc gcgaaggctg ggaagtggcg cagtcagtgc
     8101 cgacaaaagc ggtcgttgaa gcgggattta aaggcgtgga gattcgcgag gagctgaccc
     8161 gacggcggat tgcggcggta gccagctgga aggaacaacg ttgcccaaag cctgactgag
     8221 tataaaacta aacgttagca cgaaccgtcc cctcgcccct tcggggagag ggttagggtg
     8281 aggggaacag accagcaccg gtgcgaacaa tcacccccac cccggccctc tccctacaga
     8341 gcgagggaga aaacccggcc tgctcccgaa ccttttctca atcaaaagaa cacgacatac
     8401 accgcagccg ccacaataaa gcgataaatg gcgaacggga taaacgaaat gcgcttaatc
     8461 aattgcagga aggttttaat cgctatcagc gccaccacaa aagcggtgat aaacccaacg
     8521 gcaaacatcg ggatatcgcc ggttgtcagg aagccccagc ttttgtagag atcgagcgcc
     8581 gttgcgccca tcatcatcgg taccgccagc aggaacgaga actcggaagc agcgtaacgg
     8641 ctcacgccca tcagcatccc acctgaaatg gtcgccccgg aacgggagaa acccggccac
     8701 agcgccagac actggaaaca gccaatcata aacgcctgac gataggtcat atcatcaaga
     8761 cccggcgcac gcggctcttt cggcttcagg cattcggcgg caatcagcaa caaaccgcca
     8821 acgaccagcg catacatcac atttatcggg ttaaacaatg acttaatcgt gtcgtggaat
     8881 aatagcccca gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag cgttaaacga
     8941 cctttgcttt caccttcgtg ctgcaacggg cggccaaagt ggatgccaat caggccaaac
     9001 agacgccgcc agaacatcac tactaccgcc agaattgatc ctaactggat cacaacttca
     9061 aaggttttcg ccgtgtcgcc ctcaaacccc aacaagtgac cgacaataat catatggccc
     9121 gtgctggata ccggcagaaa ttctgtcaat ccttcgacca cacccaatat tgccgctatc
     9181 agcagcgagt gcatatcgct catcaataaa cccctaaatt attaaaatgt accgcttgtc
     9241 cgaactactg cagtatgacc aggttataac cgtttggttt aacagctgta aaattaatta
     9301 ttttctttca gattattgcc acgctcaatg attacgccaa cattcgccgc ccgcgccact
     9361 gcgcctggct tgctgagttt gatacgcacc cacggcgagt tgaagcgtgc taacagcagc
     9421 tccgccacct cttcagccac gcgttccacc agcgcaaaac gcgccccctc gacgtggctg
     9481 accaccgttt ctgcaatgtc agcgtaactg aggcaatccg ccacatcatc acttttcgcc
     9541 gctttacggt tatcccatgc catttcgata tcgaacacta acttctgttc gatggtctgt
     9601 tcccagtcgt aaacaccaat agtggtgatt accgaaagtt gctctataaa tacaatatcc
     9661 atcacgtcct gcctgctttt tggctaaccg gataccactt ccggcgaaat gtgcgtatta
     9721 tccacagatt catcgttgaa cacgaatttt caaaacggaa cagcttatga gtgcaatcgc
     9781 gcctggaatg atcctcatcg cgtacctctg cggctccatt tccagtgcca ttctggtttg
     9841 ccgcttgtgt gggctgcccg atccgcgaac cagcggctcc ggcaatccag gcgcaaccaa
     9901 tgtgttacgt atcggtggca agggagcagc cgtagcagta ctgattttcg acgttctgaa
     9961 aggaatgttg cctgtctggg gcgcgtatga attaggtgtc agccccttct ggctaggctt
    10021 aattgccatc gccgcctgtc ttggacacat ctggcccgtt ttcttcggtt ttaaaggagg
    10081 aaaaggcgtt gctaccgctt ttggagcaat cgcaccaatt agctgggatc tcaccggagt
    10141 aatggcggga acctggttac tgaccgtgct attgagcgga tactcgtcgc tgggagcgat
    10201 tgtcagtgca ctgattgctc cgttttatgt ctggtggttt aagccacaat tcaccttccc
    10261 ggtttcgatg ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg
    10321 gcgtcgtcag gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa aggatcccga
    10381 gtgatttctg gtggatctac atgacctgat agccttcatc gggcttgccc agccgttgct
    10441 ggcaccatgc cgccagaaat tccgcgcaga cacgaatttc atgctgcgat agagcagctc
    10501 ccggtaaaca gcccagatat tggcgctttg tgcatactcc ggcaatactt gcaccagttt
    10561 gccactctcc agaaacggca acacatccca ctcggaacgc agcataatcc ctttgccctc
    10621 cagcgcccat tgcaacacta tctcgccgct attggaggaa agatgcccgc taaccttcac
    10681 cgattttttc tcctgaccgt tccccaactc ccatattccg tgggtcatat cgcgctcttt
    10741 ggtcaccaga cagtcatgac gacttaattc ctgtaatgat tgtggctgcg gatatttttg
    10801 cagatactca ggtgctgcgc ataatattct tttatttttc gttaacagat gcgcaatata
    10861 ataatcagga atttcgtcat taatacgaat atccagatca atattatcct gcactaaatc
    10921 aatttgccga tcgaacaatt caaaatgcac ctgtaactca ggataattgc gcataagttc
    10981 ggtaatggct ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat
    11041 cccctccggg cgcgttttga tttgcgtgac gtcatcgacc agtcgctgat attgggtgag
    11101 gatttccagc gcatgttcgt aacaacgttg gccactttca gtcagcgcca cgccgcgagc
    11161 ggagcggttc agcagcgttg ttgccagcgt attttcaagg atttggatcc gcttggtgac
    11221 aaatgcaggc gtttgcccaa gcgtcgccgc tgcagcacta aaactcccgc tgtggacgat
    11281 ttctaccagt acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc
    11341 gcagttgtga taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat
    11401 attcattctt attttcgcgt tgattcacga actgttaatt acatattcac ggtggcaaaa
    11461 aatataaaac cacattttta gtgttagttt gtggcggtga atttttccag acaaatacaa
    11521 aaactggagt tgccatgatg agcgaaagta ataagcaaca gg
//
LOCUS       DAJTNV010000114        11536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNV010000114 DAJTNV010000000
VERSION     DAJTNV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..457)
                     /gene="intS"
                     /locus_tag="OO199_003374"
     CDS             complement(<1..457)
                     /gene="intS"
                     /locus_tag="OO199_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5537297.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRG"
     gene            complement(619..693)
                     /locus_tag="OO199_003375"
     tRNA            complement(619..693)
                     /locus_tag="OO199_003375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(658..660),aa:Arg,seq:cct)
     gene            complement(767..1699)
                     /locus_tag="OO199_003376"
     CDS             complement(767..1699)
                     /locus_tag="OO199_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5537298.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1993..2748
                     /gene="mlaA"
                     /locus_tag="OO199_003377"
     CDS             1993..2748
                     /gene="mlaA"
                     /locus_tag="OO199_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5537299.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2930..3604)
                     /locus_tag="OO199_003378"
     CDS             complement(2930..3604)
                     /locus_tag="OO199_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537300.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="OO199_003379"
     CDS             complement(4355..5695)
                     /gene="fadL"
                     /locus_tag="OO199_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5537301.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6067..6351
                     /locus_tag="OO199_003380"
     CDS             6067..6351
                     /locus_tag="OO199_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5537302.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6531..7841
                     /gene="fadI"
                     /locus_tag="OO199_003381"
     CDS             6531..7841
                     /gene="fadI"
                     /locus_tag="OO199_003381"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5537303.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7841..9985
                     /gene="fadJ"
                     /locus_tag="OO199_003382"
                     /pseudo
     CDS             7841..9985
                     /gene="fadJ"
                     /locus_tag="OO199_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            10188..10673
                     /gene="sixA"
                     /locus_tag="OO199_003383"
     CDS             10188..10673
                     /gene="sixA"
                     /locus_tag="OO199_003383"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5537304.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMGPCNLKMAKA
                     I"
     gene            11357..>11536
                     /locus_tag="OO199_003384"
     CDS             11357..>11536
                     /locus_tag="OO199_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5537305.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIG"
BASE COUNT         2894 a         2618 c         2836 g         3188 t
ORIGIN      
        1 caccgcgatc ttcaaatctg cggattactt ccagcagttg catcggctca atatcctgaa
       61 tctcaaggcc gccgatgatg ggtaaaatgt cgtcatcaaa catttttgca agttcagtcg
      121 cataccctac tgaccagact tgcttcttgt gttcgtacca ttccttgtaa atcgcactaa
      181 atgaattgtt gttagacgaa gcctttttcg cctttaccgg atcgatgcca gccgagatgt
      241 ctttcctcgc agtccatgct ttatcccttg cctcctgcaa agtcataagc ggatattttc
      301 cgacggtcag gattttctcc ttaccgtcaa tcttgtagcg aagctgccat acctttttcc
      361 cggatacagg gacataaagg tacaggccat taccatcgag aaggcggtat ggtttttctt
      421 tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg taaaaatccg gtgggtaaaa
      481 ttattttatc cactttttac ccgtcatgga gtgcggctgt caacgatctg acgcgaacca
      541 tgacgaactg tgaatatacg gaaggcttga tattcagggg attttgcgga ctggtacgga
      601 tgggagcgaa ctgataaatg gtgtcccctg caggaatcga acctgcaatt agcccttagg
      661 aggggctcgt tatatccatt taactaagag gacaatgcgg cgagtatacc cgctaatgga
      721 ctgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gctgggtttt
      781 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
      841 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
      901 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
      961 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     1021 cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     1081 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     1141 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     1201 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     1261 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     1321 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     1381 ttatgacgat aataaaacca aaggtataac cgagattctc cagcaagaag ctgcccggca
     1441 caccttccag ttcggcatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     1501 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttctagctct ttttcaccat
     1561 cctggcggat atgctcatga attgccatcg ctcgggaggg gagtcggtct tcatctattt
     1621 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     1681 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     1741 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     1801 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     1861 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     1921 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     1981 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     2041 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     2101 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     2161 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     2221 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     2281 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     2341 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     2401 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     2461 acgctgcgtg atgacggtgg tgatatggcg gatgctcttt acccggttct ttcctggctg
     2521 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     2581 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     2641 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     2701 aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga aacaaataaa
     2761 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     2821 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt tgtcactgac
     2881 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     2941 caaaattaac acctagtgca gatagttgat taatttcatt ttgatatttc gtctggcgat
     3001 tttgaagtct tttttcaact ttatcttcga gattcttaaa gtctttgtat ttattttcag
     3061 gatggtcaac gtcaactttg ttagcgaata aagtagaaat tatttctttt acattatcgc
     3121 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     3181 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     3241 aaccagttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcac
     3301 tcctggcgtt ttcactacaa tattgggtga gactatcacc attaatacga attcctcttt
     3361 gaatgtggta gtcgtcctga tcgtagtaac cgagaaataa gtaatcattt ccgtttttat
     3421 ttttaattac aatagcgaga gagtcgcctt ttttatcttt accgtaccag gtaaataagt
     3481 ccgttggttc gcttttcccg ctctcatcaa attccgttac cgtatgtctg agtttgtgtg
     3541 atatcttatc gaagataaaa ccaggatctc catcatatat ttcttttaat gcgctgaaga
     3601 tcatttcctt gttattgata tcatgagtct tctcaactat gttgtcatta atgatagtac
     3661 ggacgagttc tttgtcttta tagttcgcag gtaattctat tggttcttgg ttgtcgatac
     3721 caatagtaat atggtctgta tcattggagc caatatcaat ataagtaact gaggaattat
     3781 taatattaga tctataagga tcgtcgttta ctcgtatggc gttttttata agctcaagta
     3841 gatgtttaat acaggtgctt attccttcct taccattaaa aatggttttt gaactcttgt
     3901 actaatccat taatttcatc attgttattt tcattgatgg attctgggta agctgcgctg
     3961 gtattgttga ttgaaataga tggtagcatg attttattcc atgttgattg atttgttaca
     4021 aatatatatt acctaatgaa atagactatc aaaaaacgct catttattta gaacctatct
     4081 ttaatttgag tgtataagcg cacaatagcc aggggggttg agtatcctgg aaatgcagaa
     4141 aagtaaactc tttgagaagg acgagcagaa cgcttgaaaa tatgcatttc ccttcaggtt
     4201 tgtctgtgga tactacttac tgatttgaaa gggcaagctg ccatcaggct gtttgcaaaa
     4261 ctcaggaggc aagcaatgag gaaactactt tcggtgaagt gggggagtta aagtcacctg
     4321 catagcaggt gactttatct aggcgaacgc gttatcagaa cgcgtagtta aagttagtac
     4381 cgaacagcca ggctttacct tcagactcga actggtatgg gccttcgtta attttcacgc
     4441 tctgaccgtg catataagaa acaccaacgt cgactgaagc atctttatta aatgcgtaag
     4501 tcgtacctgc actcagccag aaacggtcct ggtccggaat ggagatagaa cgattctgag
     4561 ccggaactgg gctgtcatca aaggcgatac cggtacggaa ggtccagtta tcatcgtagt
     4621 aataagtggt acccaacgcg atgcggtaag catctttaaa gccttcatgt ttctggaaca
     4681 gcgtgtcgcc actggttgag gtcgctttca gctgctggaa ctgactccag ctggtgtaag
     4741 ccaggctata gtgaatcgcc cactgtggat caacacggtt ataacctgac acttcccaca
     4801 tttcaggcag gttcagcgtc agataacccg attgcgttgc gccacctgtc gcggtaggaa
     4861 ttggcaaacc gtagttatta aacgcacgat taagatcgct gctgtagtta cctttgaagt
     4921 caattttcac ttcagaacgg taggtcagtg catagcggtt atttttatcc agttcataca
     4981 ggattccggc gttccagcca aagccccact ggttaccgtt cagatgagcg attttggtat
     5041 tactgtcgat accgttggcg gtagctgcca atgcttgccc ttgaggagtt tgcccggcag
     5101 gagattgcat aatctgacca gcaaccaact gccccagatc gcctgcgaaa cgttcaattt
     5161 tcgcgcgagc gtagatggcg ttgaaaccaa gaccaaagct ccatgcatta tttaagcgat
     5221 acgcaccgct taagttcagg ttcatggttt caaggtcagt tgtacccccg acagagccgc
     5281 ctgcataagt atcattaaac tctgttgcca gaccatagtt agaggtaata gaagcgcccc
     5341 aaccaaattg gtcgttaatc ggtgcaacaa agtgcatgtt cggaacccat gccgtaggcg
     5401 cgatgttatc ggctttcagg ctacgaccag acggagacgt tccgctgata ttcacatccg
     5461 ggtcaatata aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta atcaatgcgg
     5521 ggttacggct aacgttacct gcatcatcgg caattgcgcc ttcccctgaa taagcccggc
     5581 ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc tgggtggaga
     5641 taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg ctcatgacca
     5701 taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg agcgcgaagt
     5761 gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg tcggaccagc
     5821 tagaatgttg caagtatgtt tccatctttt ttcaaatatg atctaagaaa cgcgatccag
     5881 ctggcaaaat ttggaggttg cgcactcgcg gattcgggtg aattttgttt tagatcattt
     5941 ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga gcagcacttt
     6001 ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat acagaggaaa
     6061 tcaacgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat ggatgttggc
     6121 accattatgg acaactccga ctgcaccgcg tcttacagcc gcgtgtttgc aaaccgcgca
     6181 gaagcagaac aaacgctggc ggcgctgact gaaaaatccc gtagcgtgga atccgaaccg
     6241 tgcaaaatct ccccgacttt cactgaagag tctgacggtg tgcgcctgga tatcgatttc
     6301 acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta attcctctcg
     6361 ggtacgtacg cccctgtcag ttgctggcag gggcgttttc ttttctttct tctgtcttca
     6421 ctgtgtcata gctcccactt tcctttttct ccgcttggct aaatgtaaaa aaatggttaa
     6481 gactatgatc aggtcagacc actttattta tttttttaca ggggagtgtt atgggtcagg
     6541 ttttaccact ggttacccgc cagggcgatc gtatcgccat tgttagcggt ttacgtacgc
     6601 cttttgcccg ccaggcgacg gcttttcatg gcattcccac ggttgattta gggaagatgg
     6661 tggtaggcga actgctggca cgcagcgaga tccccgctga agtgattgaa cagctggtct
     6721 ttggtcaggt cgtacaaatg cctgaagccc ccaacattgc gcgtgaaatt gttctcggta
     6781 ccggaatgaa tgtgcatacc gatgcttaca gcgtcagccg cgcttgcgct accagtttcc
     6841 aggcggttgc aaacgtcgca gaaagcctga tggcgggaac tatccgagcg gggattgccg
     6901 gtggggcaga ttcctcttcc gtattgccaa ttggcgtcag taaaaaactg gcgcacgtgc
     6961 tggttgatgt caacaaagct cgaaccatga gccagcgcct gaaactcttc tctcgcctgc
     7021 gtttgcgcga cttaatgccc gtaccgcctg cggtagcaga atattctact ggcttgcgga
     7081 tgggcgacac cgccgaacaa atggcgaaaa cctacggcat cacccgagaa cagcaagatg
     7141 cattagcgca ccgttcgcat cagcgtgccg ctcaggcatg gtcagacgga aaactcaaag
     7201 aagaggtgat gactgccttt atccctcctt ataaacaacc gcttgtcgaa gacaacaata
     7261 ttcgcggtaa ttcctcgctt gccgattacg caaagctgcg cccggcgttt gatcgcaaac
     7321 acggaacggt aacggcggca aacagtacgc cgctgaccga tggcgcggca gcggtgatcc
     7381 tgatgactga atcccgggcg aaagaattag ggctggtgcc gctggggtat ctgcgcagct
     7441 acgcatttac tgcgattgat gtctggcagg acatgttgct cggtccagcc tggtcaacac
     7501 cgctggcgct ggaacgtgcc ggtttgacga tgagcgaact gacattgatc gatatgcacg
     7561 aagcctttgc agctcagaca ctggcgaata ttcagttgct gggtagtgaa cgttttgctc
     7621 gtgaagtact ggggcgtgca catgccactg gcgaagtgga cgatagcaaa tttaacgtgc
     7681 ttggcggttc gattgcttac ggacatccct tcgcggcgac cggcgcgcgg atgattaccc
     7741 agacattgca tgaacttcgc cgtcgcggcg gtggatttgg tttagttacc gcctgtgctg
     7801 ccggtgggct tggcgcggca atggttctgg aggcggaata atggaaatgg catcagcgtt
     7861 tactcttaat gttcgtctgg acaacattgc tatcatcacc atcgacgtac cggatgagaa
     7921 aatgaatacc ctgaaggcgg aatttgcctc gcaggtgcgc gccattatta agcaactccg
     7981 tgaaaacaaa gagttgcgcg gcgtggtgtt tatctccgcg aaaccggaca attttatcgc
     8041 cggagcagac atcaacatga ttggcaactg caaaacggcg caagaagcgg aagcactggc
     8101 gcggcagggc caacagttga tggcggagat tcatgctttg cccattccgg ttatcgcggc
     8161 tattcatggc gcttgcctgg gcggtgggct ggagctggcg ctggcgtgcc acggtcgcgt
     8221 ttgtactgac gatcctaaaa cggtgctcgg tttgtctgaa gtacaacttg gattgttacc
     8281 cggttcaggc ggcacccagc gtttaccgcg tctgataggc gtcagtacag cattagagat
     8341 gattctcacc ggaaaacaac ttcgggcgaa acaggcatta aagctggggc tggtggatga
     8401 cgttgttccg cactccattt tgctggaagc cgctgttgag ctggcgaaga aggatcgccc
     8461 atcttcccgc cctctacctg tacgcgagcg tattctggcg gggccgttag gtcgtgcgct
     8521 gctgttcaaa atggtcggca agaaaacaga acacaaaact caaggcaatt atccggcgac
     8581 agaacgcatc ctggaggttg ttgaaacggg attagcgcag ggcaccagca gcggctatga
     8641 cgctgaagct cgcgcctttg gcgaactggc gatgacgccg caatcgcaag ctttgcgtag
     8701 tatctttttt gccagtacgg acgtgaagaa agatcccggt agtgatgcac cgcctgcggc
     8761 attaaacagc gtgggaattt taggtggtgg cttgatgggc ggcggtattg cttatgtcac
     8821 tgcttgtaaa gcggggcttc cggtcagaat taaagatatc aacccgcagg gcataaatca
     8881 tgcgctgaag tacagttggg atcagctgga gggcaaagtt cgccgtcgtc atctcaaagc
     8941 cagcgaacgt gacaaacagc tggcattaat ctccggaacg acggactatc gcggctttgc
     9001 ccatcgcgat ctgattattg aagcggtgtt tgaaaatctc gaattaaaac aacagatggt
     9061 ggcggaagtg gagcaaaatt gcgccgctca taccattttt gcttcgaaca cgtcatcttt
     9121 accgattggt gatatcgccg ctcacgccgc gcgacctgag caagttattg gcctgcattt
     9181 cttcagtccg gtggaaaaaa tgccgctggt agagattatt cctcatgcgg gtacatcggc
     9241 gcaaaccatc gccactacgg taaaactggc gaaaaaacag ggcaaaacgc ccattgtcgt
     9301 gcgtgacaaa gccgggtttt acgtcaatcg catcttagcg ccttacatta atgaagctat
     9361 ccgcatgctg accgaaggtg aacgggtaga gcacattgat gccgcgctag tgaaatttgg
     9421 ttttccggta ggcccaatcc aacttttgga tgaggtagga atcgacaccg ggactaaaat
     9481 tattcctgta ctggaagccg cttatggaga acgttttagc gcgcctgcaa atgttgtttc
     9541 ttcaattttg aacgacgatc gcaaaggcag aaaaaatggt cggggtttct atctttatgg
     9601 tcagaaaggg cgtaaaagca aaaaacaggt cgatcctgcc atttacccgc tcattggcgc
     9661 acaagggcag gggtgactct ccgcaccgca ggttgctgaa cggtgtgtga tgttgatgct
     9721 gaatgaagca gtacgttgtg ttgatgagca ggttatccgt agcgtgcgtg acggggatat
     9781 tggtgcggta tttggcattg gttttccgcc atttctcggt ggaccgttcc gctatatcga
     9841 ttctctcggc gcgggcgaag tggttgcaat aatgcaacga ctggccacgc agtatggttc
     9901 ccgttttacc ccttgcgagc gtttggtcga gatgggcgcg cgtggggaaa gtttttggaa
     9961 aacaactgca actgacctgc aataagaagg tcaaagctat atgaatccgc gctgaatggc
    10021 ggagtgttgg tcaaaatgta aacgcatatt gactatactt acgccattga ggtaaaaaac
    10081 agcgtttcat tcggtgaatg gataaggcac aatgccggcc accgttttct ttctctggtt
    10141 tcagatgaaa gaaaacgggc gaatctggtt aacaaaagcg gtgcaatatg caagttttta
    10201 tcatgcgtca cggcgacgca gccctcgatt ccgccagtga ttcggttcgt cctctgacca
    10261 ctaatggttg tgacgaatct cgcctgatgg cgaactggct gaaaggtcaa aaagtggaaa
    10321 tcgaacgcgt tctggtgagc ccgttcctgc gagccgagca aacactggaa gaagtagggg
    10381 attgtctgaa cctgccttcc agtgcagaag ttctgccgga gttaacgccc tgcggcgatg
    10441 ttggtctggt cagtgcctat ttgcaggcgt tgaccaatga aggtgttgct tcggtactgg
    10501 tgatctctca cttaccgtta gtcgggtatc tggttgccga gttatgtccg ggcgaaacgc
    10561 cgcctatgtt taccacctcg gccattgcca gcgtaacgct tgatgaaagc ggaaacggta
    10621 cattcaactg gcagatgggc ccgtgtaatc tgaagatggc aaaagctatc tgatgtgttc
    10681 caaattatgc ccaatgaggt agattcattg ggcataattg ctcattaggc agcggatatc
    10741 tgtcaacctc actactatcg aagactctcc ttatctataa ataaaacact ttctacattc
    10801 attcggaaat tattcttctg cgcgtatctt gatttttccc aaagttacat gttgaaatga
    10861 ataatatctg tttgacactc attgtcattt attaatacag ataatagaga aaatagagtt
    10921 gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt gtttatatat
    10981 tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt gttttttaag
    11041 ctgttgatat tcattattga tatactttct attttttcta aatacttatt ccgtattttt
    11101 taaggatgtc cttaagataa cctttcttct gtcaatatat taattaaatc tacatttact
    11161 ctttagtaaa attaaattgg atcaggggtg tggaaacatc actatgcggc cccacaccaa
    11221 tgaatacaat tttctacgac ctgttctttt tgggtcgcat gccgtattca atggctgggt
    11281 ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt aattatttat
    11341 agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg caatggtgat
    11401 gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg cacgttttta
    11461 cggcaccatt gaagactcag tgtgttctat cgttccagac gatcacaaac tggaagttga
    11521 tatgggtgat atcggt
//
LOCUS       DAJTNV010000115        11069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNV010000115 DAJTNV010000000
VERSION     DAJTNV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            50..1540
                     /locus_tag="OO199_003385"
     CDS             50..1540
                     /locus_tag="OO199_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5537306.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1592..2749
                     /locus_tag="OO199_003386"
     CDS             1592..2749
                     /locus_tag="OO199_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5537307.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2715..2837)
                     /locus_tag="OO199_003387"
     CDS             complement(2715..2837)
                     /locus_tag="OO199_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537308.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3040..4725
                     /locus_tag="OO199_003388"
                     /pseudo
     CDS             3040..4725
                     /locus_tag="OO199_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4763..7123
                     /locus_tag="OO199_003389"
     CDS             4763..7123
                     /locus_tag="OO199_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5537309.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7180..9963
                     /locus_tag="OO199_003390"
     CDS             7180..9963
                     /locus_tag="OO199_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5537310.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10324..>11069
                     /locus_tag="OO199_003391"
     CDS             10324..>11069
                     /locus_tag="OO199_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5537311.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGG"
BASE COUNT         3248 a         2465 c         2427 g         2929 t
ORIGIN      
        1 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
       61 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      121 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      181 aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg gctatgtggc
      241 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      301 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      361 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      421 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      481 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      541 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      601 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      661 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctacc
      721 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
      781 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
      841 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
      901 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
      961 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1021 ttatgacaag ctgatttccg caatggattt ctacccaaca gctcttgatg cagccgatat
     1081 cagcattcca aaagacctta agctggatgg tgttcccttg ctgccctggt tgcaagataa
     1141 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1201 tgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
     1261 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1321 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1381 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1441 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1501 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1561 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1621 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1681 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgaagcgtc
     1741 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     1801 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     1861 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     1921 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
     1981 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2041 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt atcctcttga
     2101 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     2161 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2221 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2281 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     2341 ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2401 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2461 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2521 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2581 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2641 ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta agaacagagt
     2701 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     2761 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     2821 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     2881 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     2941 atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag taaatcattg
     3001 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     3061 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     3121 aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg cgtggttaaa
     3181 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     3241 accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat ctttgtatta
     3301 atatccgtaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3361 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3421 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3481 atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3541 tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg tggaacagaa
     3601 tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg tggaacttta
     3661 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     3721 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     3781 aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat taaagagaac
     3841 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat ttattcgcgc
     3901 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     3961 ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa tttaagctgg
     4021 tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     4081 ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca acatgcggta
     4141 caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga gaacttgaac
     4201 tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc gggaaaaacc
     4261 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     4321 gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact gaaagagatt
     4381 atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt actgcatcag
     4441 gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4501 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4561 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg cttactgcgt
     4621 ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4681 tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca ataaaagccc
     4741 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     4801 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat
     4861 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     4921 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     4981 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa
     5041 aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc
     5101 aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     5161 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     5221 ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg
     5281 cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt
     5341 aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac
     5401 ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc
     5461 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5521 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5581 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5641 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     5701 gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact
     5761 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     5821 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt
     5881 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     5941 attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     6001 tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa
     6061 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct
     6121 gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga
     6181 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     6241 ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat
     6301 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6361 aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt
     6421 gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag
     6481 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     6541 caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg
     6601 ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     6661 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     6721 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     6781 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     6841 agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     6901 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     6961 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     7021 caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc
     7081 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     7141 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     7201 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     7261 gatgaaaagt taattaccgg gcaactggac aatgacttgc gatatatgat ttatccgcat
     7321 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     7381 gaagacaatg agctcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
     7441 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     7501 gatgttaatg cctatatcag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     7561 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     7621 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     7681 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
     7741 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac gccggcacaa
     7801 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     7861 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     7921 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     7981 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
     8041 gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag
     8101 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     8161 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     8221 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     8281 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     8341 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     8401 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     8461 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     8521 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
     8581 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
     8641 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
     8701 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag
     8761 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
     8821 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     8881 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     8941 aactcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     9001 cggactaata accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg
     9061 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     9121 gaccagcgtc cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac
     9181 cgcacgaaat tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct
     9241 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     9301 gcagaagaca aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat
     9361 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
     9421 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     9481 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     9541 ctacgcgtta atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg
     9601 gttgatcctc aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     9661 catgatgaac agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc
     9721 agtgagcaag aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     9781 cgtagcgttc aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct
     9841 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     9901 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
     9961 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
    10021 gtaataattt tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg
    10081 cctgattttt acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa
    10141 cacctttcca ccacccaaga attctctatt acattgtttt taatgaaaaa atatcattcc
    10201 aacaatatct cacgataaat aacattagga ttttgttatt taaacacgag tcctttgcac
    10261 ttgcttactt tatcgataaa tcctactttt ttaatgcgat ccaatcattt taaggagttt
    10321 aaaatggata agaagcaagt aacggattta aggtcggaac tactcgattc acgttttggt
    10381 gcgaagtcta tttccactat cgcagaatca aaacgttttc cgctgcacga aatgcgcgac
    10441 gatgtcgcat ttcagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    10501 ctggccactt tctgccagac ctgggacgac gaaaacgtcc acaagttgat ggatttatcc
    10561 attaacaaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
    10621 tgcgtaaaca tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt
    10681 ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg
    10741 cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc
    10801 ggtccggtgc aaatctgctg gcataaattc gcccgctact gggatgtgga gctgcgtgag
    10861 atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga agcctgcgac
    10921 gaaaatacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa ctatgagttc
    10981 ccgcaaccgc tgcacgatgc actggataaa ttccaggccg acaccggtat cgacatcgac
    11041 atgcacatcg acgccgccag cggtggctt
//
LOCUS       DAJTNV010000116        10918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNV010000116 DAJTNV010000000
VERSION     DAJTNV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            146..496
                     /locus_tag="OO199_003392"
     CDS             146..496
                     /locus_tag="OO199_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5537312.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            549..944
                     /locus_tag="OO199_003393"
     CDS             549..944
                     /locus_tag="OO199_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5537313.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            985..1728
                     /gene="cmoA"
                     /locus_tag="OO199_003394"
     CDS             985..1728
                     /gene="cmoA"
                     /locus_tag="OO199_003394"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5537314.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1725..2696
                     /gene="cmoB"
                     /locus_tag="OO199_003395"
     CDS             1725..2696
                     /gene="cmoB"
                     /locus_tag="OO199_003395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5537315.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2861..5290)
                     /gene="torZ"
                     /locus_tag="OO199_003396"
     CDS             complement(2861..5290)
                     /gene="torZ"
                     /locus_tag="OO199_003396"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5537316.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5315..6415)
                     /locus_tag="OO199_003397"
     CDS             complement(5315..6415)
                     /locus_tag="OO199_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5537317.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6803..7549)
                     /gene="cutC"
                     /locus_tag="OO199_003398"
     CDS             complement(6803..7549)
                     /gene="cutC"
                     /locus_tag="OO199_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5537318.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7563..8129)
                     /locus_tag="OO199_003399"
     CDS             complement(7563..8129)
                     /locus_tag="OO199_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5537319.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8345..10078
                     /gene="argS"
                     /locus_tag="OO199_003400"
     CDS             8345..10078
                     /gene="argS"
                     /locus_tag="OO199_003400"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5537320.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10255..10743
                     /locus_tag="OO199_003401"
     CDS             10255..10743
                     /locus_tag="OO199_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5537321.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2668 a         2787 c         2602 g         2861 t
ORIGIN      
        1 ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca ctcatagcta
       61 accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt taggaaccat
      121 ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg ttcatgcatc
      181 acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag ttccggtaca
      241 tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg atatgacgca
      301 aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc agaccactac
      361 gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg tacataccct
      421 gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta ttccacagca
      481 atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc attttaaagg
      541 gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta atgaagttct
      601 cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac ggtggtttta
      661 gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt cccatcgcga
      721 ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg catatttgcg
      781 gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac cgtctgtttc
      841 gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg gtgctggcga
      901 atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca atacgccact
      961 ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc tgcccctatc
     1021 gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc ggatatgatc
     1081 cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt agccgagcgc
     1141 ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc ggcgacgctc
     1201 tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga caactccccg
     1261 gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac gccagtagac
     1321 gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt ggtgctgaat
     1381 tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa aatttatcaa
     1441 ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga agatgccaaa
     1501 gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg ttacagcgaa
     1561 ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga ttccgtggaa
     1621 acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg gttccagtgc
     1681 tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc gactttggta
     1741 acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg ctgcccgcgc
     1801 agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg tccaacacgg
     1861 tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc gtaaccgccg
     1921 aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt atgcggaacc
     1981 tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat accgaatggc
     2041 gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc ggacgcacca
     2101 ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc gcaggcgcgc
     2161 acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa gcggtgcgta
     2221 aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa cagcttccgg
     2281 cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc cgttcgccgc
     2341 tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg gtgctggaaa
     2401 cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt tacgcgcaaa
     2461 tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg aagaagtgtg
     2521 gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag cagcgacgca
     2581 ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat ccgagtaaga
     2641 cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag ccgtaaaggt
     2701 ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc cttgctaatt
     2761 caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca gttttacgtt
     2821 tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga acagcaggct
     2881 tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac accagcgcag
     2941 agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt aacacgttcg
     3001 cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca tgcacgcata
     3061 ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga ccacgtttgt
     3121 tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct tcggtgtgaa
     3181 tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca tagttaagct
     3241 gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc tcgtcggcgg
     3301 tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc ttatagccaa
     3361 atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc agcgcatttt
     3421 tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc tcgctgcggc
     3481 gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata gtgacgcgtt
     3541 gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc atttcatctt
     3601 taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga tcggcaaaca
     3661 cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc ggcacaatat
     3721 gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat gtggtgatcg
     3781 gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa acgacgatca
     3841 tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg gtaaagttac
     3901 cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta ccgttatgct
     3961 gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga atcgccgtca
     4021 ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac atttccggca
     4081 gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag aaaccaaaac
     4141 cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc atccagtgtt
     4201 tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt gtgcgattcg
     4261 ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta atttccgccg
     4321 tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct tcaaactgcg
     4381 gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt gtcatcaggg
     4441 tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg atccaggtgg
     4501 cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca atcactggtt
     4561 tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta ctgctccagg
     4621 caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg ctgttttcca
     4681 gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc acatgcggca
     4741 tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa ccgcccgcca
     4801 ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa gagcgccagc
     4861 cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc tcatgttgct
     4921 catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct tcgccacgac
     4981 ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga tgctgaatac
     5041 gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt atggttttcg
     5101 ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc atcgctcccc
     5161 aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg agcggtgctg
     5221 ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat tcacgtcttg
     5281 ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta atatctttgg
     5341 cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg ctggttcgtg
     5401 cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg gcggcaatag
     5461 aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc catagcggtt
     5521 tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga cgccattgat
     5581 tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata ccttcttcac
     5641 ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt tcactgccct
     5701 gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca acgacggtga
     5761 gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg aaaggataaa
     5821 tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc gccgcgttgt
     5881 catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg atacaggttt
     5941 cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc gaggcaatat
     6001 ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct tttaattctt
     6061 tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca ctgtctattt
     6121 tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca aataaataat
     6181 ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct ttctggttcg
     6241 aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg caggaaaggc
     6301 aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac ccgccgccac
     6361 caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct cgtatagatt
     6421 cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca acgaattaac
     6481 cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat gatttattac
     6541 aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatata tatttatcag
     6601 ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta gcacaacgct
     6661 atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat actgctcagg
     6721 gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca tcatattggg
     6781 cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt cctttcattt
     6841 cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt tcatctgatg
     6901 acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct cccgcggagc
     6961 tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca cggactcctg
     7021 ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt tttgataaac
     7081 cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc aattccgcaa
     7141 gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga aaagtcactg
     7201 ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca ttcccgtcaa
     7261 catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg cgcacatcct
     7321 caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt gggcgaatta
     7381 tcggatgcac agggatcgtc acccgctggc gcacggattt cagtaccccc aacgacggcg
     7441 ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg ttttgctgcg
     7501 ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt tttgctcctt
     7561 aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg aaacttaata
     7621 gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg ctcacctttt
     7681 ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga aagcaatgcc
     7741 agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat atgttcccaa
     7801 ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct ccacattgga aaactgccag
     7861 tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc attgatcatt
     7921 ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc agcggtgacg
     7981 ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc caaccccaga
     8041 cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc aatatcctgc
     8101 agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt aaaagcgtta
     8161 atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct gatgcaatag
     8221 tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg cagtatactc
     8281 ccgccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa tgtaaggtat
     8341 tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga ttgcggcagg
     8401 cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt tcggcgacta
     8461 tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc gacaattagc
     8521 agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg agatcgccgg
     8581 tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg ttcagcaggc
     8641 gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg tggttgacta
     8701 ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta ccattattgg
     8761 tgacacagca gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg caaaccacgt
     8821 cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc agcagcagga
     8881 aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg cgaaaaagca
     8941 ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag
     9001 cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca tgacgcagaa
     9061 ccagatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga tgggcgaaag
     9121 cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag gtctggcagt
     9181 agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg aaggcgaacc
     9241 gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca ctgatatcgc
     9301 ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt acatcgactc
     9361 ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag gctatgtacc
     9421 ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag acggcaaacc
     9481 gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac ctgctggatg aagccctgga
     9541 gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg agctggaaaa
     9601 actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac
     9661 cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata ccgcgccata
     9721 catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa ttaacgaaga
     9781 gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac tggcagctcg
     9841 cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc cgcatgtaat
     9901 gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc actgcccgat
     9961 cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac aactgacagc
    10021 gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc gtatgtaatc
    10081 gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt tattgccatc
    10141 taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa gaggccagaa
    10201 gcgtaatatc caggcccagg aaaacgataa cggttcaatt taaggaatac cgaagtgttt
    10261 aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga agatacggtt
    10321 atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa ttcagatgat
    10381 gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatct cgtcgatgcg
    10441 caattaaata aagcctatgg tgaagcttat cgttatatag aacaagtgcc acgcacaggt
    10501 gcaaaaaaac ttgatactga acaacttaac ttgcttaaaa aatcacagcg agcctggctg
    10561 gattttaggg acaaagaatg tgaattaatc ctttcaaatg aggatgttca ggatttaagt
    10621 gacccctatt ctgaatcaga atggctatca tgtatgatca tacagaccaa tacgcgaact
    10681 cgccagttgc agctataccg taactctgaa gatttttatc caagcccttt gacaagagga
    10741 taattcacat ctttttggca tgttttgttg caagctattc ctgataaatg attgcaacaa
    10801 aacatccagc cttttgactg agttattaaa catactcgcg agcgcgtaat tttttgtctt
    10861 tcagcgataa ttcacaatca cttcattccg ttgtaccttt agcggtggaa ttaaccgc
//
LOCUS       DAJTNV010000117        10858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNV010000117 DAJTNV010000000
VERSION     DAJTNV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            238..1692
                     /gene="amn"
                     /locus_tag="OO199_003402"
     CDS             238..1692
                     /gene="amn"
                     /locus_tag="OO199_003402"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5537322.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2035..2751
                     /locus_tag="OO199_003403"
     CDS             2035..2751
                     /locus_tag="OO199_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5537323.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3207..3282)
                     /locus_tag="OO199_003404"
     tRNA            complement(3207..3282)
                     /locus_tag="OO199_003404"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3247..3249),aa:Asn,seq:gtt)
     gene            complement(3383..5010)
                     /gene="yeeO"
                     /locus_tag="OO199_003405"
                     /pseudo
     CDS             complement(3383..5010)
                     /gene="yeeO"
                     /locus_tag="OO199_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5015..5090
                     /locus_tag="OO199_003406"
     tRNA            5015..5090
                     /locus_tag="OO199_003406"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5048..5050,aa:Asn,seq:gtt)
     gene            complement(5128..6078)
                     /gene="cbl"
                     /locus_tag="OO199_003407"
     CDS             complement(5128..6078)
                     /gene="cbl"
                     /locus_tag="OO199_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5537324.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6180..7097)
                     /gene="nac"
                     /locus_tag="OO199_003408"
     CDS             complement(6180..7097)
                     /gene="nac"
                     /locus_tag="OO199_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5537325.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7425..7500
                     /locus_tag="OO199_003409"
     tRNA            7425..7500
                     /locus_tag="OO199_003409"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7458..7460,aa:Asn,seq:gtt)
     gene            complement(7556..8491)
                     /gene="ldtA"
                     /locus_tag="OO199_003410"
     CDS             complement(7556..8491)
                     /gene="ldtA"
                     /locus_tag="OO199_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5537326.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8553..9632)
                     /gene="cobT"
                     /locus_tag="OO199_003411"
     CDS             complement(8553..9632)
                     /gene="cobT"
                     /locus_tag="OO199_003411"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5537327.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9644..10387)
                     /gene="cobS"
                     /locus_tag="OO199_003412"
     CDS             complement(9644..10387)
                     /gene="cobS"
                     /locus_tag="OO199_003412"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5537328.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10384..>10858)
                     /gene="cobU"
                     /locus_tag="OO199_003413"
     CDS             complement(10384..>10858)
                     /gene="cobU"
                     /locus_tag="OO199_003413"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5537329.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3027 a         2771 c         2337 g         2723 t
ORIGIN      
        1 atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact ggcatagcat
       61 cgccatttat ttgctggctg gatgcctgat ttctgcaatg accgctttat tgatgaaaga
      121 caatcaacgc tcgtgatagc ctggcgaaga tcatccgatc ttcgccttat acttttgttt
      181 cacattcctg tgacatacta tcggatgtgc ggtaattgta tggaacagga gacacacatg
      241 aataataagg gctccggtct gaccccagct caggcactgg ataaactcga cgcgctgtat
      301 gagcaatctg tagttgcttt acgcaacgcc attggcaaat atattacaat tggcgaatta
      361 cctgatgaaa acgcccgtaa acaaggtctt tttgtctatc catcactgac cgtaacctgg
      421 gacggtagca caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac tcacgcaggc
      481 agctacacca ccacgattac tcgccctact ctctttcgtt cgtatctgaa tgagcaacta
      541 acgttgcttt atcaggatta tggtgcgcat atctctgtgc aaccctcgca acatgaaatc
      601 ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat gagcgctgat
      661 ttaactcgct acttcccgac aacagaactg gcgcaaatcg gcgatgaaac tgcagacggc
      721 atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg cgtcgatttt
      781 tccctcgcac ggttgcgcca ttataccggt acgccagtag aacattttca accgttcgtc
      841 ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg cagccagatc
      901 ctcgatcctg atagtcccta cattgccctt tcttgtgctg gcgggaactg gatcactgcc
      961 gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca ccagatgcca
     1021 gcatggcatt taattaccgc tgatggtcag ggtattactc tggtgaatat tggcgtggga
     1081 ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga tatctggttg
     1141 atgattggtc actgtggcgg attacgtgaa agccaggcca ttggagatta tgtccttgca
     1201 cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga tatacctatt
     1261 ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt cagcggcagg
     1321 cctggtgagg aagtcaaaca gcggctgcgt acaggtactg tggtaaccac agatgacagg
     1381 aactgggagt tacgttactc agcttctgca cttcgtttta acttaagccg ggccgtagca
     1441 attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt gccatacggg
     1501 acactactgt gtgtttcaga taaaccgtta catggtgaga ttaagcttcc cggccaggct
     1561 aaccgttttt atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg ggcgatcgat
     1621 ttgctgcgcg cagaaggcga ccgactgcat tcacgtaaat tacgaacctt taatgagccg
     1681 ccgttccgat aataagttat gaaccttctt atcagaaata gatgtgaagg attaagtaag
     1741 acatctggca aatagcctgc aataaacgcg ggctgcttgc ttccttgggc agatgcgagt
     1801 tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt
     1861 ataaaaaatc ccgccaacaa cattagtttt tataaaatta aatataagat tatggcttta
     1921 gaatattttt atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa
     1981 cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga
     2041 cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt
     2101 tatgcaaaat tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa
     2161 ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac
     2221 gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtacaggga
     2281 cgttatgaag gctttggtcc taatggctca atgattattg ctgagacatt gacttcaaat
     2341 gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga
     2401 gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca
     2461 gaccctgacc atatttttga aattttgctt gaagctgaag ttgatgttcg tgatgtaact
     2521 gaagaagaag gtaatattgt tatttatact gaacctgctg accttcataa aggaatcgcg
     2581 gcgctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa
     2641 tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt
     2701 gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatttt
     2761 aaagaaatct gtctttatgg cagatttctt taatctcata taattcttat aaaaaatata
     2821 atatttaact cgtcatattg cttataaaaa atccccgtcc ccagagaaat aatatttatt
     2881 aaaatggcag gtcttctttt tctgattacc gaagacaaag tggcaattac gtatagtttc
     2941 ccgataaaga cgcgacagct acgtcccgca taaggcattt ttctctttat ctttgtacgg
     3001 tacttcatgg aacagaattt ttgaccttgt gaatcgtgat atctgttggg gaggggcaat
     3061 ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg
     3121 ccactttttt acaaagtggc cgggtaaaaa taatttttat tttttaactg tcctgagtct
     3181 catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac cagtgacata
     3241 cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga
     3301 atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa atcgtttgct
     3361 gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg
     3421 gctcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca
     3481 cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca ccccagccaa
     3541 gcatgattcc ctgcacataa ccgactacaa cccggcaacc ccacatgctc aacatagaaa
     3601 cccacatggc gtaacgggca tcacgagcac ccttaaatcc agcgggtagc acccatgagg
     3661 cggaccaaat aggcataaat aaagcattta gctaaatcag aatcacaacg acgtgtttaa
     3721 cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg
     3781 cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga atctctgctt
     3841 gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg
     3901 cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt ccggcaataa
     3961 cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca
     4021 ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg ctaaaattca
     4081 gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca
     4141 cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac ccagccccga
     4201 ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct
     4261 accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat
     4321 aagagtgatg gcagcggctg gataactgag tacggtcagc tccagataag tcaacgccag
     4381 tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg
     4441 atgaattagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt
     4501 cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgctacaa cgacagtagt
     4561 accaagatcg atagctgcaa aaaaagccat aatgaccata ttgaagctgt ccgccaaccc
     4621 cacgccggcc atcgcatctt ttcccagcca actgaccaga aaagtgctca ggccccccat
     4681 caacaggaca caggcgttct ccatgaagat aggaacagca agcggggtta tctcgcgcta
     4741 gaacaacact ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg
     4801 taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa
     4861 tgatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca
     4921 acaaactgtt gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat
     4981 atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg
     5041 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa
     5101 aagcccgctt ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt
     5161 ttccatcacc tggcgcttga tatcctcaac tgacagtcct gcattacaaa attccagaaa
     5221 gcgccaaaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga cagtattagc
     5281 atcgaaaaga tgccgcgtat ccaggcggat taaattctct tcctcttgtt cgccgctgga
     5341 ttgttcggca actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga
     5401 atcctgtgca cttaatacaa tatctgccgg caaacctttg cgggtaaatg cgtcatcagt
     5461 gcgtgagcgc cccgtaattc cctgtcggta agtaattaac ggccacttcg ctattgattc
     5521 cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg
     5581 ccaacaaaac cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat
     5641 accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat
     5701 tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct
     5761 ataacgtgcc tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa
     5821 caggtctgca agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag
     5881 taatgctttg cccggttcag tcatccccag cagtcgctta cctcgtcgaa caaatatttc
     5941 gatgccaaat tcatcctcca gttcccgaat atgacggcta acgcctgact gtgaggtaaa
     6001 aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt
     6061 tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta ttgttaaagt
     6121 ctaatgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct
     6181 tagctcacca attgccactg ccttttttcc atcactggag aactaatcac tgacattaac
     6241 aactctttca ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa atttaaggac
     6301 aaagagagac tcattgaagg agtggtaatg cgtgacatcc acccatttac tgcgccacat
     6361 aacgaacgcg cagccgattc aggtaatact gcaacgccca tgccgctggc aatcgctgcg
     6421 gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa
     6481 aaagcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa aaggttcatt
     6541 tgcgcaatag cattaacatc aacgttttgc cccgggcaat cttgagttcc taccaggaaa
     6601 agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc
     6661 accgccatat cgagttggtg atttatcagt ttttcgttaa gcactgcacc actattttca
     6721 tgaagataga taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg
     6781 atggatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga
     6841 ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg
     6901 gtatagataa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc
     6961 tgttgattta actcaccttc cagtatggca acctgctggc tgagcgctgg ttgtgcgata
     7021 tgcaatactt cagcagcctg ggtcaggcta ccaatatcta caatttttac gaagtatttc
     7081 aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg ccgccattac
     7141 ttacaaccag tatgcaagat gcctgccagt tctattttgg cgttgatgcg caagctaacc
     7201 aactgtcaaa taagagatta tgatagattc gtcattggcc ctttttatca gcagtcgcat
     7261 tcacctgccc tataaaagga gggtatgcac cacgatggtt cattacccaa taagattgaa
     7321 agctcaccac tttgttgaaa ttggcagcaa acaaacaaaa aaaatgcatt tcaccctttg
     7381 acatcaccat gcactgccat taatatgcgc cccgttcaca cgattcctct gtagttcagt
     7441 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     7501 aattcaaaaa agcctgcttt cgagcaggct ttttactttc taattaccaa cgctcttaaa
     7561 acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt tgcaacgcag
     7621 catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa gatggggtta
     7681 ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac agcggctcat
     7741 gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc tgatcaataa
     7801 tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg tcattacgca
     7861 gacgaataca gccctgactt acacggagcc caataccaaa attggcattg gtaccgtgga
     7921 tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg ggccccgcag
     7981 gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccgggtg ttcggcgttg
     8041 gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta cgcggggttt
     8101 ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg tctggcggat
     8161 agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacgaaca gtggcgggca
     8221 aaatcagttg ctgcggaatg gtgagttgtg agccaggctt cggcaaaaaa acatcagcgc
     8281 ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca aaagtctcca
     8341 gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact aaacggctac
     8401 cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa agcagagcaa
     8461 atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg aatactatcc
     8521 gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga agtcgtattc
     8581 cccggtagaa caatattact ggcagcaagt tcgcccatgt tgttgtatat cgcacaagca
     8641 gcttcgatga tggacatcgc cagagctgca ccactcccct cacctaaacg catatccata
     8701 ttgagataag gctccagccc caaatgcgag agcgctatac gcgcgccttt ttctgccgac
     8761 aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc tgcgagcgca
     8821 gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc agcacctaac
     8881 atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac atcaacacca
     8941 tcctgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat tttattagcc
     9001 agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc ccggccagtg
     9061 attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc acctacacca
     9121 aacagcgtga caccgttttt cgccagctcc cgcgtataac atatgacgtc caaaagcaac
     9181 ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct acctcgtgcg
     9241 acacgcatgt tgataagccc ggggataggc tcagcagtat caataccaac atcaattacg
     9301 tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc acgggtcata
     9361 ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc ctcccagacg
     9421 ccgtgatcgg cacacataac cagtaccgct tttttgccca catgcggtat gccattcacc
     9481 cccggcattc ctgccagttg tatggcaagc gcctccagct ttcctaggct accaacaggt
     9541 ttgaataacc cgtcaacatg ccgttgtgca cgcgacatag cggcagaatt gatggctggg
     9601 atcgtattca gtaaatcggc aattatttgc atgtcacgtt tctttatagc agtgccagca
     9661 ggaagaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt tgcccgccca
     9721 gcgtacgttt gagaagctga ccgaggatga aaatcgccac catcgtcacc accatagcag
     9781 ccacaccatg catacccagc aataatacgg cagcaaaaat agcggctaag ccgagtgtga
     9841 cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct tcttcccgcg
     9901 cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca catgctgccg
     9961 ccagcgatgc aaggatcggt tcgccacgca gagccaactc gcttaacacc agaatctttg
    10021 ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta tcacgcatga
    10081 tttccaacat gcgatcgcga ctacgtgcag aaaatacgcc atcacaggta tcagcgaggc
    10141 cgtccagatg gaattccccg gtcatcagcg ccagcacaag tacgctaaac agtgccgcca
    10201 gtggtgcgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc gcgccaagca
    10261 ataatccaat caaaggaaaa gtaataatac cgcgagaata atgctcgaaa tccagtccct
    10321 gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc caaaataatt
    10381 tgctcatttg atttttactc caatacccga aaccaccagc catacttcat ttgccgcagc
    10441 ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc
    10501 cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg ggcaacgttg
    10561 gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg cctgataatc
    10621 ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca ccattgtggt
    10681 aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta attcatcaag
    10741 atgttgccag cgctccactg tgcgccagtg ctccgggcgg ctttgccgat gatgttctat
    10801 ccgtgcagcc atctcatcat caaggatttg cgaggtagcg atatacagaa cctgtgaa
//
LOCUS       DAJTNV010000118        10683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNV010000118 DAJTNV010000000
VERSION     DAJTNV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(69..1418)
                     /gene="lysC"
                     /locus_tag="OO199_003414"
     CDS             complement(69..1418)
                     /gene="lysC"
                     /locus_tag="OO199_003414"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5537330.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1943..3592
                     /gene="pgi"
                     /locus_tag="OO199_003415"
     CDS             1943..3592
                     /gene="pgi"
                     /locus_tag="OO199_003415"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5537331.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4054..4296
                     /locus_tag="OO199_003416"
     CDS             4054..4296
                     /locus_tag="OO199_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537332.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4410..5048
                     /locus_tag="OO199_003417"
     CDS             4410..5048
                     /locus_tag="OO199_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5537333.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5045..5782
                     /locus_tag="OO199_003418"
     CDS             5045..5782
                     /locus_tag="OO199_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5537334.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5782..7878
                     /locus_tag="OO199_003419"
     CDS             5782..7878
                     /locus_tag="OO199_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5537335.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7925..8203)
                     /locus_tag="OO199_003420"
     CDS             complement(7925..8203)
                     /locus_tag="OO199_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537336.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8204..8347
                     /locus_tag="OO199_003421"
     CDS             8204..8347
                     /locus_tag="OO199_003421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537337.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8473..8883
                     /gene="psiE"
                     /locus_tag="OO199_003422"
     CDS             8473..8883
                     /gene="psiE"
                     /locus_tag="OO199_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5537338.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8927..10402)
                     /gene="xylE"
                     /locus_tag="OO199_003423"
     CDS             complement(8927..10402)
                     /gene="xylE"
                     /locus_tag="OO199_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5537339.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2714 a         2728 c         2766 g         2475 t
ORIGIN      
        1 ctcaggctac ctcatagtga caagaaaatc aatacggccc gaaatatagc ttccaggcca
       61 tacagtattt actcaaacaa attactatgc agtttttgca ccacctgctc ggcatcttcg
      121 ccgggcacca ggaagcacag gttatggctg gatgcgccgt aacaaatcat gcgaatgttg
      181 aacggttcca gtacgccgaa tacctctttg ccaacgccgc aggcttttga caggtcattg
      241 ccaatcaacg cgaccagcgc cagaccttct tccacctcca cccggcacag tgcggaaagc
      301 tccatcagca gagattgcgt cagcaacgta tcgccagtgg aggttgaacc ggtggtatca
      361 agggttaatg ccacgctcac ttctgacgtg gtgattaagt ctaccgaaat attatgccgc
      421 gcgaggatgc cgaaaacttc cgcgaggaag ccgcgagaat gcagcatatt caggctgtgc
      481 aaagtgagca gagtctgatt gcgacgaagc gccagcgcgc ggaacagcgg cggattttca
      541 gttttattgc acaccagcgt accacctgcg cgtgggtctt tgctggagcc gacaaagacc
      601 gggatatcgc tgcgtactgc gggtagcaac gttgccggat gcagtacttt tgcaccaaaa
      661 gttgccatct ctgccgcttc ggcaaacgcg atttcatcaa tgcgttttgc tgcggaaact
      721 acgcgtggat cggtggtgta gatgcccggg acgtcggtcc agatatcaac acgagatgcg
      781 tgtaaagcct ccgccagcaa ggctgccgta taatcgctgc ctccacggcc aagcgtcgtt
      841 gtacgacctt tattttcgct accgataaat ccctgggtga tcactaagcc ttcattgagg
      901 cgtgggagca gctgcagcgc ggccagttcc gccagcgcgg ctacatctgg ctctgcacga
      961 ccaaatcggt cgttggtacg catcacttta cgtacatcaa accactgtgc ctgaacatcg
     1021 cgttcgcgca ggatttcgac aaacagcagg gtcgacatca gctcgccgtg gctgaccagc
     1081 tcatctgtca gcgccggaga cgttgccagc gccgccgctt ctgccagaac agtaatgttc
     1141 tccagcagac gttcaatctc ttcacggata acgttcgggt aacgcagacg ttccagaatg
     1201 gcaaactgga tgttgcgaat agcgtcgagt ttttcgaatc gctcgccaag ttccagccct
     1261 tccgctaaag cgaccagcag attagtgata ccagcagaag ccgagaggac tactaaacgc
     1321 acgttggcat cagaaagcac aatatcagcg ctgcggttca tggcgtcaaa atcagctacg
     1381 ctggtaccgc caaatttgga gacaacaatt tcagacataa ctacctcgtg tcaggggatc
     1441 cattttcagc cttggcacaa gggaagagcg gaagacgggt gggcgcagag cgatacttcg
     1501 ctactatttt cacccagaag tgctccacca cttgcgaaac gcccgactgc gaacgcttct
     1561 ggtgacaacc caggggattc agcccctgta gccgatgatg aacgtggcca gccgttcaat
     1621 cacctcggcg atgcaccccc tcaggtgtta tcacaggact ggctcctcca acaccgttac
     1681 ttgggcaacg cgcctcttct ggcctgcgct agcgcaggta gtacatttat aaataaaggg
     1741 tgagcggggc ggttgtcaac gacggggtca tgcggatttt tcatccactc ctggcggtca
     1801 gtagttcagc taataaatgc ttcactgcgc taagggttta cactcaacat tacactaacg
     1861 gcactaaaac catcacattt ttctgtgact ggcgctacaa tcttccaaag tcacaattct
     1921 caaaatcaga agagtattgc taatgaaaaa catcaatcca acgcagaccg ctgcctggca
     1981 ggcactacag aaacacttcg atgaaatgaa agacgttacg atcgccgatc tttttgctaa
     2041 agacggcgat cgtttttcta agttctccgc aaccttcgac gatcagatgc tggtggatta
     2101 ctccaaaaac cgcatcactg aagagacgct ggcgaaatta caggatctgg cgaaagagtg
     2161 cgatctggcg ggcgcgatta agtcgatgtt ctctggcgag aagatcaacc gcactgaaaa
     2221 ccgcgccgtg ctgcacgtag cgctgcgtaa tcgtagcaat accccgattc tggttgatgg
     2281 caaagatgtt atgccagaag tcaacgcggt gctggagaag atgaaaacct tctcggaagc
     2341 gattatctcc ggtgagtgga agggttatac cggcaaagca atcactgacg tagtgaacat
     2401 cgggatcggc ggttctgacc tcggcccgta catggtgacc gaagcactgc gtccgtacaa
     2461 aaaccacctg aacatgcact ttgtttctaa cgtcgatggg actcacatct cggaagtgct
     2521 gaaaaaagta aacccggaaa ccacgctgtt cctggtagca tctaaaacct tcaccactca
     2581 ggaaaccatg accaacgccc atagcgcgcg tgactggttc ctgaaagcgg caggtgatga
     2641 aaaacacgtt gcgaaacatt ttgcggcgct ctcaaccaat gccaaagccg ttggcgagtt
     2701 tggtattgat actgccaaca tgttcgagtt ctgggactgg gtcggcggcc gttactcttt
     2761 gtggtcagcg attggcctgt cgattgttct ctccatcggc tttgataact tcgttgaact
     2821 gctttctggc gcacacgcaa tggacaagca tttctccacc acgcctgccg agaaaaacct
     2881 gcctgtcctg ctggcgctga ttggcatctg gtacaacaat ttctttggtg cggaaactga
     2941 agcgattctg ccgtatgacc agtatatgca ccgtttcgcg gcgtacttcc agcagggcaa
     3001 tatggagtcc aacggtaagt atgttgaccg taacggtaac gttgtggatt accagactgg
     3061 cccgattatc tggggtgaac caggcactaa cggtcagcac gcgttctacc agctgatcca
     3121 ccagggaacc aaaatggtac cgtgcgattt catcgccccg gctatcaccc ataacccgct
     3181 ctctgatcac caccagaaac tgctgtctaa cttcttcgcc cagaccgaag cgctggcgtt
     3241 tggtaaatcc cgcgaagtgg ttgagcagga atatcgcgat cagggtaaag atccggcaac
     3301 gcttgactac gtggtgccgt tcaaagtgtt cgaaggtaac cgcccgacca actccatcct
     3361 gctgcgcgaa atcactccgt tcagcctggg tgcgttgatt gcgctgtatg agcacaaaat
     3421 ctttactcag ggcgtgatcc tgaacatctt caccttcgac cagtggggcg tggagctggg
     3481 taaacaactg gcaaaccgta ttctgccaga gctgaaagat gataaagaaa tcagcagcca
     3541 cgatagctcg accaatggtc tgattaaccg ctataaagcg tggcgcggtt aatcatcatg
     3601 cgtgcctgat gccgatatgt aggccggata aggcgtttac gccgcatccg gcaaccggag
     3661 cctgatgcca atatcgacat taatgcatag tatgaggagt taaataacct taaaaacaat
     3721 gccgacaata agtcggcatt gttttatcag ataaatcccc ttgtctgtga tttaacggaa
     3781 atcataccgt gaggttaatc ctaaaataga tttttaatcg ttgtttattt cggagaatac
     3841 gcagattaat tacttttgtt tttattttaa gcttatgatt tttattgtta tttaaatata
     3901 agttgaaact tatatttgat attcattcca attatcctaa aactccatcg ctaattcccc
     3961 gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc cgcccgaaat gtttttgggt
     4021 aaatctccat tcattcaatg aagggaaatt gttatgaaaa aagttctgta tggcattttt
     4081 gccatatctg cgcttgcggc gacttctgcg tgggctgcac ctgtacaggt gggcgaagcg
     4141 gcagagtcgg cagcaacgtc ggtttcggcg gggagttcct ccgcgaccag cgtcagcacc
     4201 gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg gcggtgatgg ttctaatacc
     4261 gggaccatca caaccacgac caccagtacc cagtaataaa gtatgtatcc ccaaaataat
     4321 ttgagtcatt gcatctgtgg cttgaagtat gaagggattt accataacca cactccggtg
     4381 tggttattct gcccctctgg agaagagtcg tgaagcgacc tgcactcatt cttatctgcc
     4441 tgctattaca ggcctgttca gccacgacta aagagctggg caattcactg tgggacagtc
     4501 tgttcggcac gccaggcgta cagctgacgg acgatgatat tcaaaatatg ccctacgcca
     4561 gccagtacat gcagcttaat ggcgggccgc aattgtttgt ggtgctggcc ttcgctgaag
     4621 acggacaaca aaaatgggtc actcaggatc aagccactct tgtcacacaa catggtcgtc
     4681 tggtgaagac tttgcttggc ggcgacaacc tgattgaagt gaataacctt gccgccgacc
     4741 cgctgattaa acccgcacaa attgttgatg gtgcaacatg gacgcgcacg atgggctgga
     4801 ccgagtacca gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg gatggcaccg
     4861 ataccgtcaa ggtcggcagc gatgaaaccc tggttcgcgt gctggacgaa gaagtctcca
     4921 ccgaccaggc gcgctggcat aaccgctact ggatcgacag cgaagggcaa attcgccagt
     4981 cggaacagta tctcggcgcg gattatttcc cggtgaaaac cactctcatc aaggcggcaa
     5041 aacaatgatt aaacaaacta ttgtcgcgtt gcttttgagt gtgggagcgt catcggtctt
     5101 cgcggcagga accgtcaagg tgttcagcaa tggcagcagt gaggccaaaa cgctaacggg
     5161 cgcagagcat ttaatcgatc tggtagggca acctcgactg gcaaacagct ggtggcctgg
     5221 tgcggtgatt agcgaagagc tggcaacggc ggcagcattg cgtcagcagc aggcgttgct
     5281 gacacggctg gcagaacagg gcgcagattc cagcaccgac gatgccgccg ccattaacgc
     5341 cttacgccag caaatccagg cattgaaagt gacaggcagg caaaaaatca atcttgatcc
     5401 cgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg cagggcaact acacgctgtg
     5461 ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt atcagccatc ctggcaatca
     5521 gccattcact ccaggtcgcg acgtggcgag ctatctctct gaccagagcc tgctcagcgg
     5581 tgcggatcgc agctacgcgt gggtcgttta cccggacgga cgcacgcaaa aagcgccggt
     5641 ggcttactgg aacaagcgtc acgtagagcc gatgcccggc agcattattt atgttggcct
     5701 cgcagattcc gtctggagtg agacgcctga tgcccttaac gccgacattc ttcagactct
     5761 gacgcagcgg ataccccaat aatgaaaaaa agacatctgc ttagcttact ggcgctgggc
     5821 attagcacag cttgctacgg cgaaacatat cctgcgccca ttggtccgtc gcagtcggat
     5881 ttcggtggcg taggattatt acaaactccc accgcgcgta tggcacggga aggggagttg
     5941 agtctgaact atcgcgataa cgatcagtac cgttattact cagcttcagt gcaactcttc
     6001 ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca cccggcagta cagcagcgtc
     6061 gaagcgttct ctggcgatca aacgtataaa gataaagcct tcgatctcaa actgcgtttg
     6121 tgggaagaga gttactggct gccgcaagtg gcggttggtg cgcgggatat cggcggtacg
     6181 gggctgtttg atgcggaata tcttgttgcc agcaaagcct gggggccgtt cgattttacg
     6241 ctcggcctgg gctggggata tctgggcacc agcggtaatg taaaaaatcc gctctgttca
     6301 gccagtgata aatattgcta tcgcgataac agctacaaac aggcgggatc tatcgacggt
     6361 agccagatgt tccacggtcc tgcctcactg tttggcggcg tggaatacca gacgccctgg
     6421 caaccgctgc gtctgaaact ggagtatgaa ggtaataatt atcagcagga ttttgccggg
     6481 aagctggagc aaaaaagtaa gtttaacgtc ggggcgattt atcgcgttac cgattgggcc
     6541 gacgttaacc ttagctatga acgtggcaac acctttatgt ttggcgtcac gctgcgcacc
     6601 aactttaacg atctgcgccc gtcctacatc gataacgccc gcccgcaata tcaaccgcag
     6661 ccgcaggatg ccattttgca gcattcggta gtggcgaatc agttaacgtt gctgaaatac
     6721 aacgccggac ttgccgatcc gcagatccag gcgaaaggcg atacgctgta tgttaccggc
     6781 gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc gtgctaatcg gatcgtgatg
     6841 aacgatctgc cggatgggat caaaacgatc cgcattacgg aaaatcgcct taacatgccg
     6901 caagtgacga cggaaaccga tgtcgccagc ctgaaaaatc atctcgccgg agagccgttg
     6961 ggccacgaaa cgacgctggc gcacaaacgc gtcgagccag tggttccgaa gtccaccgag
     7021 cagggctggt atatcgacaa atcacgcttt gatttccata tcgatccggt gctgaaccag
     7081 tcggtcggtg gcccggaaaa cttttacatg tatcagctgg gcgtgatggg aacggcagat
     7141 ttgtggctga cggaccatct actgaccacc ggcagcctgt ttgcaaatct tgccaacaac
     7201 tacgacaagt ttaactacac caatccaccg caggactcgc acttaccgcg cgttcgtacc
     7261 catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata acctgcaagc caactacttc
     7321 cagcatctgg gcaacggctt ctacggtcag gtctatggag gttatctcga aaccatgttt
     7381 ggcggtgcgg gggccgaagt gttgtatcgt ccgctggaca gtaactgggc gtttggtctg
     7441 gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa aagatatgat gaaattcacc
     7501 gactacagcg tgaaaaccgg acatctgacc gcctactgga cgccatcttt cgcccaggac
     7561 gtgttggtga aagccagcgt cgggcagtat ctggcagggg ataaaggcgg cacgctggag
     7621 atcgctaaac gctttgacag cggcgtggtg gtgggtggct atgccacgat caccaacgtt
     7681 tcgaaagagg agtacggcga aggggacttc accaaaggcg tgtatgtctc ggtaccgttg
     7741 gatctcttct cgtctggccc gacacgcagc cgtgcggcga ttggctggac gccgctgacg
     7801 cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt atgacatgac cagcgaccgt
     7861 agcgtaaatt tccgctaagt catgggaaag gtgccagttt tcgcactcac tggcaccctc
     7921 attcttaatg acctgttgag ccatcaggaa cgcagttcac ccccggttta cgatctggtg
     7981 tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat cggttgcagg tctggttgcc
     8041 tgagcgactg ggccatcatt gggctggaga cattgctttg cggtaggtcg gctttatcag
     8101 cagtaccgag cggaccagca taagcaggaa gaacagagac tgataacatc aaagcagcaa
     8161 aataaggctt catttttacc acctttatca ggttacgttt catttgttcc agaggaacat
     8221 tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct gaatgggtga tttttgaatt
     8281 accggctttg gtgcggtttg tcttgccgga tgcgccgcca ggcgcggctt atccggccta
     8341 cgggtaggta tatccggctt tggtggaggc gcgctccaaa tccaggttga acaaaacata
     8401 cacaaaaaat atagatctcc gtcacatttt tgcgttatac aggaagctcg ccactgtgaa
     8461 ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg agtttatctc caccattttg
     8521 cagaccgtac tcaatcttgg cctgctgtgc ctcggcctga ttttggttgt cttcctcggc
     8581 aaagaaacgg tgcatctggc tgatgtgctg ttcgcgccag aacaaaccag caaatatgag
     8641 ctggtagaag gactggtggt ttactttctc tatttcgaat ttatcgcgct gattgtgaag
     8701 tactttcagt ccggttttca cttcccgcta cgttactttg tctatattgg gatcaccgcc
     8761 attgtgcgct tgatcatcgt cgatcataaa tcgccactgg atgtgttgat ctactcggct
     8821 gcgatcctgt tactggtgat caccctgtgg ctgtgtaatt cgaaacggct gaagcgggag
     8881 taaaaagaca gcacgccgaa atggcgcggc gtgctggaca ggaagattac agcgtagcag
     8941 tttgttgtgt tttcttcgtt tccggttccc agagcgcttc cagctcctca agggttttac
     9001 ctttggtttc cgggacaaat ttccacataa acagtgctgc cagaacgccc atacaaccgt
     9061 aaatccagta ggagaaaccg ttgtggaaat gggccaccag ccaggagttt ttgtccatca
     9121 tcgggaaggt ccaggagacg aagtagttcg ccagccactg ggccgccacc gcgattgcca
     9181 gcgctttacc acgaatagca ttcgggaaga tttccgacag cagtacccag cataccggac
     9241 cccaggacat ggcaaaggcg gcaacataga acagcatcga cagtagcgcc acaatacccg
     9301 atgcctgagt gtaaaacgcg gtaccgaggc taaacatacc gattgccatt ccgagtgcgc
     9361 cgataatttg cagtggctta cgaccaaatt tatccacagt cataattgcc agtacggtaa
     9421 aggtgaggtt gataactccg acaataatgg tctgcaacag cgcgatatcc gtgctggccc
     9481 ccagcgtttt gaacacttcc ggcgcgtagt acagcaccac attgatgccg acaaattgct
     9541 ggaagatgga gagcataacg ccgattacaa tcacgcccac gccaaacatc agcagacgac
     9601 caccggtttt gcggccatga tccagggagt gtttaatttc ctgtactgcc tgagttgcaa
     9661 gcgtgttgcc cataattttg cgcaggatac tttccgtctg ttcttgcttg ccgcgtgaca
     9721 tcagccagcg aggactttct ggcacggtat acagcagcat taagaacagc agtgcaggga
     9781 tacattccga ggcaaacata taacgcctgc cgtcagtatt cagccagctg gcatcaccgg
     9841 aacgggcaat aaaatagttt acgcagtaaa ctaaaagttg cccgaaaata atcgcaaact
     9901 ggttaaatga aaccagtttt ccgcgaatat gagctggagc cagttccgca atatacattg
     9961 gtgaaagcat tgaggctaaa ccaacgccaa taccgccaat aatacgataa ataacaaatt
    10021 ccgggacata acctgccaga taaataggta ctgtgttgtc cgggtttata gaggtaaaac
    10081 caagttctgg ccaggcagaa cctacaccag aaataaaaaa caggacagca gaaatcttaa
    10141 gtgaatcacg acggccgaag cggttactgc aataaccacc gagggcaccg ccgatgatgc
    10201 aaccaatcag agcgctggct acgcaaaatc ctaacaggga gttggcagcg gattcactta
    10261 agttttgtgg agcaacaaag acggtattga gtgactcaac agtaccggaa ataacggcgg
    10321 tgtcgtagcc aaataataaa ccacctaatg tagcgactaa ggtaatcgaa aatatataac
    10381 tggaattata ctgggtattc attcagacct gccttagacc attctgatct tttatcagtg
    10441 cattgatgtt ggaattgtca ggcagttaag gcaattatca ttttttacaa tgcgtttacg
    10501 taatgtcttt tctgtgatct taattgtgat aattatcaaa aattgatgaa aacaatacta
    10561 ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa
    10621 ataataagtt ctatgatggg aatattaacc ggcgaacgat tcagattgca gacgaaagaa
    10681 aaa
//
LOCUS       DAJTNV010000119        10651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNV010000119 DAJTNV010000000
VERSION     DAJTNV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..213)
                     /locus_tag="OO199_003424"
     CDS             complement(<1..213)
                     /locus_tag="OO199_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5537340.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="OO199_003425"
     CDS             704..835
                     /locus_tag="OO199_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5537341.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="OO199_003426"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="OO199_003426"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5537342.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="OO199_003427"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="OO199_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3268..4320
                     /locus_tag="OO199_003428"
     CDS             3268..4320
                     /locus_tag="OO199_003428"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5537343.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4396..5829
                     /locus_tag="OO199_003429"
     CDS             4396..5829
                     /locus_tag="OO199_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5537344.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6012..8192
                     /locus_tag="OO199_003430"
     CDS             6012..8192
                     /locus_tag="OO199_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5537345.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8333..9616)
                     /locus_tag="OO199_003431"
     CDS             complement(8333..9616)
                     /locus_tag="OO199_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5537346.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9751..10632)
                     /locus_tag="OO199_003432"
     CDS             complement(9751..10632)
                     /locus_tag="OO199_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5537347.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2612 a         2456 c         2655 g         2928 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accatatcac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
     3301 ggaacctcga gggccgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
     3361 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
     3421 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
     3481 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
     3541 aactgcggca atatgctgtc tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca
     3601 gcgacttcgc cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa
     3661 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc
     3721 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc
     3781 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
     3841 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
     3901 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg
     3961 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
     4021 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
     4081 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
     4141 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa
     4201 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     4261 cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga
     4321 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     4381 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     4441 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     4501 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
     4561 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
     4621 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     4681 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacttat tgcctatctg
     4741 ctgattggta aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat
     4801 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     4861 atcatcaaca gcgtggcgat ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     4921 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     4981 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     5041 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
     5101 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
     5161 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     5221 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     5281 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     5341 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     5401 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
     5461 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     5521 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     5581 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     5641 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     5701 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     5761 accttcttgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     5821 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     5881 ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt cctgcaactc
     5941 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     6001 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     6061 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     6121 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     6181 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     6241 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     6301 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     6361 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     6421 tgatggctag cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     6481 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     6541 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     6601 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     6661 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     6721 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     6781 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     6841 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     6901 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     6961 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     7021 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     7081 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     7141 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     7201 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     7261 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     7321 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     7381 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     7441 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     7501 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     7561 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     7621 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     7681 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     7741 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     7801 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     7861 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     7921 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     7981 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     8041 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     8101 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     8161 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     8221 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     8281 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     8341 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     8401 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     8461 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     8521 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     8581 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     8641 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     8701 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     8761 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     8821 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     8881 taacgttgtt aatctgtact tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     8941 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     9001 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     9061 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     9121 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     9181 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     9241 ccgttccctg atactcccca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     9301 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     9361 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     9421 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     9481 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     9541 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     9601 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     9661 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     9721 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     9781 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     9841 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     9901 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     9961 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    10021 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    10081 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
    10141 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
    10201 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    10261 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    10321 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    10381 cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt atccattgag
    10441 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    10501 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    10561 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    10621 tctcgctgtc agaggcttgt gtttgtgtcc t
//
LOCUS       DAJTNV010000120        10538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNV010000120 DAJTNV010000000
VERSION     DAJTNV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..518)
                     /locus_tag="OO199_003433"
     CDS             complement(<1..518)
                     /locus_tag="OO199_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5537348.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLS"
     gene            complement(515..1918)
                     /gene="baeS"
                     /locus_tag="OO199_003434"
     CDS             complement(515..1918)
                     /gene="baeS"
                     /locus_tag="OO199_003434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5537349.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(1915..3330)
                     /locus_tag="OO199_003435"
     CDS             complement(1915..3330)
                     /locus_tag="OO199_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537350.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3331..6396)
                     /gene="mdtC"
                     /locus_tag="OO199_003436"
     CDS             complement(3331..6396)
                     /gene="mdtC"
                     /locus_tag="OO199_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5537351.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6397..9519)
                     /gene="mdtB"
                     /locus_tag="OO199_003437"
                     /pseudo
     CDS             complement(6397..9519)
                     /gene="mdtB"
                     /locus_tag="OO199_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9519..>10538)
                     /gene="mdtA"
                     /locus_tag="OO199_003438"
     CDS             complement(9519..>10538)
                     /gene="mdtA"
                     /locus_tag="OO199_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5537352.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAK
                     KGARS"
BASE COUNT         2422 a         2994 c         2896 g         2226 t
ORIGIN      
        1 gagagcgttt taagcagacg aaattccgca ggcgtcaggt caagcatttt accgcgccat
       61 gaagcctgaa aacgaccttc gtcgataatc aacgggcttt cggcatcctg ttgctgaagt
      121 tcgcgttgcg gtttacaacg gcgcaaaatg gttttgacac gcgctaccac ttcccgtggg
      181 ctgtacggct tgcagatgta gtcatcggcg ccaatctcca gccccagcag gcggtcgatc
      241 tcttcgattt ttgccgtcac catcacgatc ggaacatcgg aaaaacgacg aatttcccgg
      301 cataatgtca ggccatcggt gccagggagc atcagatcca acaggatcag atccggtggt
      361 gtctggcgca catacgccag tacctgatcg ccgtggctga taagcgtcgg cgcatagctc
      421 gcagcacgca gataatcaat gagcaactgc cccagcttcg gttcatcttc cacgatcaaa
      481 atacgcggtg tgttttcgtc gattggtaac tcggtcatac ttctctctgt aaatcccgtt
      541 ccagcggtaa ctctactgta atgcttaccc cgccaaaagg cgaatgggcg gcaataatgc
      601 ggccattatg ggcttcaaca atgttcaggc aaatcgccag ccccagcccg gaaccgccgc
      661 tggcgcggtt gcgggaacct tcggtgcgat aaaaacgttc aaacaatttt tgtagctgat
      721 cgtcactgac acctggcgca ctgtcggcaa aggtcaggcg caccgttttg tcgtgctgct
      781 cggcagagat tttcaggctg ccgccgctgt cagtgtagcg caggctgttt tccagtaagt
      841 tattgaataa ctgcattaaa cggtcgcgat cgccaaatac ggtgatactg tctggcaggg
      901 aaaattgcag tttcaggcca cggctggcga atcgttcgcg aaatgcgccg cccgccactt
      961 ccagcagtgg gatcaaatct accggtgctt tttgataggc gagagcgcct tcatcagaca
     1021 tcgacaactg atgaagatcg tcaaccagtt tggtcagtgt accgacctcc gcctgtaaag
     1081 acgccaccgt ctccggcgtg aatttgcgca cgccatcctg aatggcttcc agttcaccgc
     1141 gcagcaccgc taatggcgta cgcagttcgt gagaaatatc ggccataaaa tcgcgccgca
     1201 tttgctggtt tttctccagc gtgctggcga gctggttgaa gtcttgcgcc agtttgccca
     1261 gttcatcttc actggtgggc gttacgcggg tagtgaaatc gcccgccgcc agtttgtgcg
     1321 tgccatcgac aagtcgtttt accggtgcca gcaaaccgcg cgccagcaga aaggtggcga
     1381 gcgccgcgag taacgttgcc agggcgacga tcagccagct ggtttgccgc tgttgtttat
     1441 cgaaattgat atcagtattg cgggtcagcc gctcaacagg ggaggcaatc accgcgccca
     1501 cttccgcacc gttgaccaga atgggtcgcc gtgtaccgtc aggtggaatc ggcgctcgcg
     1561 gaccaaccag tactttgttg ttttgatcga ccacccagaa ctgggtacgc cagccgtgcg
     1621 gcggcatacc cgggccgggt ttatcttccg aattatcgtg ttcaaatgaa cgcaggatct
     1681 gaaagacaaa gcgatcgttg ttgcgcagga agcgccagtt accatgctgc gcatactgct
     1741 cgccaagcgc atcacttaac agctgtaatc gctgttcatt gccgtgtttg atgtaatcaa
     1801 taaagccacg ctcaaaactg atacgcaccg cccagtgcat actgatcagc aagacaatgc
     1861 aggtggcgaa aatcgccaga aacagtttgc cggtaatacc gggtcgccag aacttcattg
     1921 cgcgctcctt tttcgccgcg aaatagctac attttgatgc gtatcgttcg gcactctggc
     1981 aaagatgaac gccggaaggg cgatgataaa cgccatgcta agccaggtgt acataaagac
     2041 ggtttgtgtg gtgccgctgt cgacgctgat atgctgcgaa ccaaaaagcc ccagcaacag
     2101 cccggcgatg gtgacgccga tactcatcga caattgcata atcatcgaca gcaggctgtt
     2161 tccgctgctc gccagattgt ccgggagatc tttcagcgtc agggtgttca tggaggagaa
     2221 acgggtcgag ttgaccatcc cttgtaaaaa caggacgaac ggcaaaacgt agtaccagcc
     2281 caacagagcg gtagtcataa acaacagggt gaccagcgac agacccagcg tggtcgctac
     2341 cagtacccga cgataaccaa agcgattcac cacctgtacc acaattcgct tcattcccat
     2401 gctgccaagc accatcggga tcatcatcag tccggcatga aacggcgaga aaccgaggcc
     2461 aatttgcagg aaaaccggtg tcataaaggg caacatgcca ctgccaatac gtccggcaaa
     2521 gctccccgcc aggcccagcg aaaaggtacg agtgcggaac agtttcagac tgaacagggc
     2581 gcggttgtta tttctggcgt gcagcagata aagcaccagt gccaccacgc caactgcgac
     2641 caggccagca atcgccagcg gcgataaacc tgtaccttta ctgccgtcca gcgccagggt
     2701 taataccgcc atgccaaccg ccagcaataa aaagccggaa agatcaaagc gccgcgtctg
     2761 catggtgtag ttcggcatta acatcaatgt ggcgatcgca ccgataatcc ccaccggaat
     2821 gttgatcaaa aagatccagt gccacgacgc gtattctacc agcagaccgc cgagcgccgg
     2881 accgagcagc ggaccgacct gaccgggtaa cgtgacaaag gtcatcgccg ccatatattg
     2941 ctcgcgcggt acgattttca tcaccgtcaa cctgccgacc ggcaccatca tcgcaccgcc
     3001 aacgccctgt aacgcgcgtg ccagcaacag ttcgttcagc gtgccggaaa gcgcgcaaaa
     3061 cagtgaaccg agagtaaaca gcacgatggc ggtaaagaaa atattgcgca cgccgacttt
     3121 gtccgccagc cagccgctgg cgggcagcat caccgccacg gtcagcacat aagagacaat
     3181 gaccatgtgc atatgcaacg gactttcccc gaggctttgc gccattgagg gaagggcggt
     3241 gtttacgatg gtggtgtcca gcgactgcat aaagaagccg aaagccacaa tccataattg
     3301 ccagcgggtg ctgtcgggaa gatctgtcat ttactcggtt accgtttgtt taggtttacg
     3361 cgaaaaacgc agccgcagac ggtcgaaaaa gagatacacc accggcgtgg tatacagcgt
     3421 caggagctgg ctcatcacca gaccgccgac aatggtgatc tccaggggtt gccgcagctc
     3481 cgagccgtca ccgcccgaca ataccagcgg cagcgcgcca aacagcgccg ccagggtggt
     3541 catcataatc gggcgaaaac gcagcagaca ggcctggaaa atagcttcct gcggcgtcag
     3601 gttaccgtgc cgttgggctt caagcgcaaa atcgaccatc ataatggcgt ttttcttcac
     3661 gatgccgatt aatagcatga tccctatcag ggcgattagg ctgaacgggg cattgaacag
     3721 ctccagcgcc aacagcgctc caacgcccgc cgagggcagg gtggagagaa tcgtcagcgg
     3781 atgtacgtaa ctctcgtaaa ggatacccag cacgatatac accgtggcga tggcggcgat
     3841 aatcaggatc acctgcgagt tcatcgtctc ctggaaaacc tgcgccgtgc cggcaaaact
     3901 gccgcgcacc gtcgaaggca caccaagctg ggtcattgcg cgatcgatcg ccgcactggc
     3961 gtccgagagc gattttccgg tcggcaggtt gaacgagatg gtcgaggccg ccgataatcc
     4021 ctgatgattc accgatagtg gggcattcgc cggttgccat ttagcgaaat acgacagcgg
     4081 gatcgctttg ccttcgttat tgataacgaa cattttttcc agcgcactga tgtcctgggt
     4141 atagcgcgga tccacttcca tcaccacttt atactggttc atcggctggt aaatggtcga
     4201 gatttgccgc tgaccgaagg cgttatttaa cagactgttg gcggcctgta cgtcgattcc
     4261 cagccgtgcc atggtgtcgc ggtcgtaaac cagattcatc tccgcgccgt tatcctgctg
     4321 atcggagttc acgtccgcca gttccggcaa cgtcgccagt tttttgcgga ttttcggctc
     4381 ccattcgcgc agtgccgcca ggtcgtcgga taacaacgtg tactggtagc tggcgttcga
     4441 ctgacgccca ccaacgcgaa tatcttgtac cgccatcagg aacagattcg cccccggttc
     4501 tttcgccagc ttcacgcgca gacggtcgat aatttgctgc gccgtttcgc tgcgttcgtc
     4561 gcgtggcttg agggtgataa acatcatccc actgttaact cgtgaaccgc cggtaaagcc
     4621 ggtgacatta tccactgccg gatcgtcacg gataattttc atgaaatcct gcaatttgcc
     4681 gcgcatcgcc tgaaacgaaa tactctgatc cgcctgtatc ccacccatca gtacgccggt
     4741 gtcctgctcc gggaagaagg ttttcgggat cgagatattc agcgcaatgg tgccaagcag
     4801 caccacgccc accagacggg tatgattgag cacccatttc agcgacttgc cgtagccttg
     4861 ttgcagggct accaacatgc ggccaaaacc acgcagtcgc ttttgctcac gcggcttgct
     4921 ggcttttagc atccagccgc acatcattgg cgttagcgtc agggaaacca gcaacgaaat
     4981 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5041 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5101 gccgacttcg cgagtacctt gcagcgcggc ctgtagtggt ttcatccccg cttccagatg
     5161 acgggcaatg ttttccagca ccacaatggc gtcatccacc acgaaaccag tagcaatggt
     5221 gagcgccatc agcgaaagat tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5281 accaatcagc gaaaccggca ccgcaacggc gggaataata gtggcgcgac ccgagcgcag
     5341 gaataaaaag accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5401 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5461 cggaatggtc tcctgcaact ctggtaattt tgcccggatg ctgtctaccg tctggataat
     5521 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5581 ggcgttgcgc acatcctgca ctgagtcagt caccgtcgcc acatcgccca gacgaaccgc
     5641 gccgccgttg ttgtagtgaa taatcagcgg ctgatattca gcggcggttt tcagttcatc
     5701 gttggtctgg atctgccagc ggtgagtgcc atcttccagc gccccctgcg gtttacgcac
     5761 gttggcattg ctgatggcag tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     5821 ctgcggggtc agcccgacgc gcacggcggg cagcgagctg ccaccgacat cgacatcacc
     5881 gacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     5941 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6001 cgctttgcga taggtcgggc ggctgggcat cccgctgggc agcaaacttt gtgcagcgtt
     6061 gatcgccgcc tgcacatcac gcgctgcgcc gttgatatcc cggtcaaaat caaactgcaa
     6121 aataatgcgc gtgctgccga gcgaactgct ggaggtcatt tcactaaccc cggcaatgcg
     6181 cccaagtgag cgctccagcg gcgtggcaac ggaagacgcc attgtctccg gcgacgcacc
     6241 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6301 cagcatacgg aaacccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6361 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6421 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6481 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6541 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6601 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6661 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     6721 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     6781 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     6841 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     6901 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     6961 ctagaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatcgc
     7021 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg ttgttaccgg
     7081 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgctcaattt tggcaattga
     7141 gcttaacggc accacgccac cgtcgctgct ggtcaggcga atggtatcca gcgccgcgag
     7201 gcctggggta ttttcggtat tgtgctccag caccacgcga tactggttag cctgagtata
     7261 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga cgtccgccat
     7321 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttt
     7381 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7441 ctgtggcacc caggtactga gcgcatccag tgacgtggcc tgcaaggtaa actggtactg
     7501 ggtgcggctg acctgagtat cgatggtcag gtcctgcgtt ggttgcagga agagatcgac
     7561 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7621 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7681 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     7741 cgcgacctgg cgttgtcgct gggccatatt ggcaaaggag ctggattgcg gtgcctgcaa
     7801 agtgccctga ataatgccgt tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     7861 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     7921 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     7981 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8041 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8101 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8161 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8221 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8281 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8341 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8401 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8461 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8521 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8581 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8641 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     8701 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     8761 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgaccgcacg
     8821 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     8881 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     8941 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9001 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9061 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9121 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9181 ggcgacattg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9241 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9301 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9361 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9421 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9481 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9541 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9601 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9661 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
     9721 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
     9781 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
     9841 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
     9901 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
     9961 ttcgaattgg tgcgatccca ggcttctacc atcagcggtt ttccggcttt ttgcgcctgc
    10021 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10081 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacttgc
    10141 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10201 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10261 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10321 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10381 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10441 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10501 gtaacagtat tagcggcggt aatggtgcca agcccggt
//
LOCUS       DAJTNV010000121         9942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNV010000121 DAJTNV010000000
VERSION     DAJTNV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO199_003439"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO199_003439"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5537353.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO199_003440"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO199_003440"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5537354.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO199_003441"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO199_003441"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5537355.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO199_003442"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO199_003442"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5537356.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OO199_003443"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OO199_003443"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5537357.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OO199_003444"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OO199_003444"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5537358.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OO199_003445"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OO199_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OO199_003446"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OO199_003446"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5537359.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OO199_003447"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OO199_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5537360.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9942)
                     /locus_tag="OO199_003448"
     CDS             complement(8974..>9942)
                     /locus_tag="OO199_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5537361.1"
                     /translation="GDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSHLYG
                     GVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSSVAV
                     YGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQSIIE
                     QTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVSVPG
                     VYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMPFEE
                     AARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2345 a         2675 c         2504 g         2418 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atagcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg cc
//
LOCUS       DAJTNV010000122         9932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNV010000122 DAJTNV010000000
VERSION     DAJTNV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..339
                     /locus_tag="OO199_003449"
     CDS             <1..339
                     /locus_tag="OO199_003449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537362.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="OO199_003450"
     CDS             527..1642
                     /locus_tag="OO199_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537363.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="OO199_003451"
     CDS             2321..2539
                     /locus_tag="OO199_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5537364.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="OO199_003452"
     CDS             2757..2999
                     /locus_tag="OO199_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537365.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO199_003453"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO199_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5537366.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO199_003454"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO199_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5537367.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO199_003455"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO199_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5537368.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO199_003456"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO199_003456"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5537369.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="OO199_003457"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="OO199_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5537370.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2491 a         2396 c         2545 g         2500 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttcgt ga
//
LOCUS       DAJTNV010000123         9783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNV010000123 DAJTNV010000000
VERSION     DAJTNV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..143
                     /locus_tag="OO199_003458"
     CDS             <1..143
                     /locus_tag="OO199_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5537371.1"
                     /translation="AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            complement(194..514)
                     /gene="psiF"
                     /locus_tag="OO199_003459"
     CDS             complement(194..514)
                     /gene="psiF"
                     /locus_tag="OO199_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5537372.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(633..2048)
                     /gene="phoA"
                     /locus_tag="OO199_003460"
     CDS             complement(633..2048)
                     /gene="phoA"
                     /locus_tag="OO199_003460"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5537373.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="OO199_003461"
     CDS             complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="OO199_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5537374.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2591..2794)
                     /locus_tag="OO199_003462"
     CDS             complement(2591..2794)
                     /locus_tag="OO199_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537375.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2872..3966
                     /gene="ddlA"
                     /locus_tag="OO199_003463"
     CDS             2872..3966
                     /gene="ddlA"
                     /locus_tag="OO199_003463"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5537376.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3990..4202)
                     /locus_tag="OO199_003464"
     CDS             complement(3990..4202)
                     /locus_tag="OO199_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5537377.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4462..4770
                     /locus_tag="OO199_003465"
     CDS             4462..4770
                     /locus_tag="OO199_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5537378.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="OO199_003466"
     CDS             complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="OO199_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5537379.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="OO199_003467"
     CDS             complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="OO199_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5537380.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7508..8665
                     /gene="ampH"
                     /locus_tag="OO199_003468"
     CDS             7508..8665
                     /gene="ampH"
                     /locus_tag="OO199_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5537381.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8666..9169)
                     /locus_tag="OO199_003469"
     CDS             complement(8666..9169)
                     /locus_tag="OO199_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5537382.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9257..>9783)
                     /locus_tag="OO199_003470"
     CDS             complement(9257..>9783)
                     /locus_tag="OO199_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5537383.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2437 a         2493 c         2333 g         2520 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 tggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcaatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
      241 cacctttcag agcttgttgc gttgcttgat tattacattc acgcatcttt tgctgctgtg
      301 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
      361 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
      421 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
      481 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
      541 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
      601 ccggcagcga aaattcactg ccgggcgcag ttttatttca gccccagagc ggctttcatg
      661 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
      721 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga attcccgtaa
      781 ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc cggcgctttg
      841 gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
      901 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
      961 tcgccaattt gcccacaagg gttcgcagcg tgatcctgtt tatcgattga cgcaccttca
     1021 acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     1081 tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtataggt gactgcgggc
     1141 ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg catattgccg
     1201 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     1261 gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     1321 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
     1381 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     1441 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga agtcacatgt
     1501 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     1561 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     1621 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     1681 tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt gtattgcccg
     1741 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     1801 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     1861 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     1921 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     1981 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     2041 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     2101 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacaagg
     2161 atgcttcaat aacttcttta cgtaatcgcg cagcagctct gtatcgtcgt caggaatgct
     2221 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     2281 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     2341 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     2401 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     2461 cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg caaatatttt
     2521 ttgtttgatt accagcgaaa cgtttctctt atttaaataa ttacccacac aaaatatagg
     2581 cttttaatta tcaaccttta ttataaataa aagctatgaa atatattctc ttcgttaact
     2641 tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc
     2701 cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta
     2761 cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg
     2821 ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa
     2881 ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg
     2941 gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt
     3001 gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat
     3061 cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat
     3121 gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc
     3181 ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc
     3241 aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc
     3301 accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc
     3361 gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt
     3421 gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag
     3481 tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg
     3541 atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc
     3601 tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa
     3661 gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg
     3721 gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg
     3781 tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc
     3841 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     3901 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     3961 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     4021 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     4081 taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat
     4141 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     4201 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     4261 gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca
     4321 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     4381 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     4441 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     4501 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     4561 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     4621 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     4681 accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta
     4741 tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg
     4801 ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     4861 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     4921 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     4981 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     5041 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     5101 ttttcctggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     5161 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     5221 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     5281 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     5341 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     5401 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     5461 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     5521 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     5581 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     5641 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt
     5701 tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg
     5761 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     5821 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     5881 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     5941 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     6001 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     6061 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     6121 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     6181 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     6241 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     6301 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     6361 gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg
     6421 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     6481 gcgttaccag caccggcagg aaggcgatca acgtcattat ggcgttgata aaactgacgc
     6541 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     6601 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     6661 agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc actgcgatca
     6721 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     6781 gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga
     6841 ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc
     6901 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     6961 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     7021 ggatctgccc ggaagcgccg gtgatatgtg ccacccagtc acccccaccg gcttgccaga
     7081 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga
     7141 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     7201 ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     7261 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     7321 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     7381 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     7441 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     7501 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     7561 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     7621 tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg
     7681 taaccagcgc gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca
     7741 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     7801 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     7861 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     7921 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     7981 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     8041 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     8101 gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa
     8161 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     8221 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     8281 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     8341 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     8401 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     8461 agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     8521 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     8581 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     8641 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     8701 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     8761 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     8821 gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta
     8881 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     8941 aaccatcagc cagccccata atataaggag cctgggtaat accgataagt acgttttctt
     9001 ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac
     9061 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     9121 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     9181 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     9241 ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat
     9301 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     9361 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     9421 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     9481 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     9541 aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     9601 caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca
     9661 gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt
     9721 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     9781 agc
//
LOCUS       DAJTNV010000124         9646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNV010000124 DAJTNV010000000
VERSION     DAJTNV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO199_003471"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO199_003471"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5537384.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO199_003472"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO199_003472"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5537385.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO199_003473"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO199_003473"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5537386.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO199_003474"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO199_003474"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5537387.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO199_003475"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO199_003475"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5537388.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO199_003476"
     CDS             5060..6193
                     /locus_tag="OO199_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5537389.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO199_003477"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO199_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5537390.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO199_003478"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO199_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5537391.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO199_003479"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO199_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5537392.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO199_003480"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO199_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5537393.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO199_003481"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO199_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5537394.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO199_003482"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO199_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5537395.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2263 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctttt
//
LOCUS       DAJTNV010000125         9627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNV010000125 DAJTNV010000000
VERSION     DAJTNV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO199_003483"
     CDS             complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO199_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5537396.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO199_003484"
     CDS             complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO199_003484"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5537397.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2165..3199
                     /gene="rhaT"
                     /locus_tag="OO199_003485"
                     /pseudo
     CDS             2165..3199
                     /gene="rhaT"
                     /locus_tag="OO199_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO199_003486"
     CDS             complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO199_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5537398.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO199_003487"
     CDS             complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO199_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5537399.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5242..6711
                     /gene="rhaB"
                     /locus_tag="OO199_003488"
     CDS             5242..6711
                     /gene="rhaB"
                     /locus_tag="OO199_003488"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5537400.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6708..7967
                     /gene="rhaA"
                     /locus_tag="OO199_003489"
     CDS             6708..7967
                     /gene="rhaA"
                     /locus_tag="OO199_003489"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5537401.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8209..9033
                     /gene="rhaD"
                     /locus_tag="OO199_003490"
                     /pseudo
     CDS             8209..9033
                     /gene="rhaD"
                     /locus_tag="OO199_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9043..9357
                     /gene="rhaM"
                     /locus_tag="OO199_003491"
     CDS             9043..9357
                     /gene="rhaM"
                     /locus_tag="OO199_003491"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5537402.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2490 g         2270 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
     1081 gacattcatt taatcaataa aagtgatcaa aatcacatca attgaaacgc tgttttattt
     1141 ttataagacc attgttttta tttttaaatt gatcacaaaa aaaccaccgc cgttggcgat
     1201 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat
     1261 tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct
     1321 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
     1381 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
     1441 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
     1501 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
     1561 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
     1621 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
     1681 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
     1741 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
     1801 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
     1861 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
     1921 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
     1981 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
     2041 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
     2101 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
     2161 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
     2221 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
     2281 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
     2341 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
     2401 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat
     2461 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
     2521 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact
     2581 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
     2641 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
     2701 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
     2761 tgaagccgct gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt
     2821 catcatgggc ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt
     2881 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
     2941 gttactctcg gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg
     3001 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
     3061 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg
     3121 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg
     3181 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
     3241 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
     3301 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
     3361 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
     3421 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
     3481 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
     3541 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
     3601 agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac
     3661 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
     3721 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
     3781 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
     3841 aatataaaag agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc
     3901 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc
     3961 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
     4021 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
     4081 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc
     4141 cctggcgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
     4201 tactgtcgct gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta
     4261 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg
     4321 tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg
     4381 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
     4441 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
     4501 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
     4561 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag
     4621 acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
     4681 atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca
     4741 gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc
     4801 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
     4861 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag
     4921 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
     4981 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aactacgtaa
     5041 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
     5101 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
     5161 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
     5221 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
     5281 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
     5341 gaaatccatc gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg
     5401 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
     5461 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
     5521 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag
     5581 gcacaacagc aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc
     5641 ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac
     5701 attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac
     5761 tgggaatata ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac
     5821 gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg
     5881 ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
     5941 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
     6001 tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
     6061 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
     6121 ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac
     6181 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat
     6241 cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt
     6301 cgggaaacgg cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac
     6361 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
     6421 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
     6481 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
     6541 atcggcatcg agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
     6601 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
     6661 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
     6721 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
     6781 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
     6841 tttccggttt tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt
     6901 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
     6961 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
     7021 tctcgcgcga ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
     7081 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
     7141 ttacgctctc gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
     7201 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
     7261 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
     7321 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
     7381 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
     7441 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
     7501 cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc
     7561 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
     7621 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
     7681 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata
     7741 tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag
     7801 cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc
     7861 aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc gaatggctgg
     7921 agagcgtgcg ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg
     7981 cggcacgagc gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc
     8041 ccggccctct ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg
     8101 aacaccgctc cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa
     8161 gccgcaccat gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt
     8221 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgccta gctgaaaggc
     8281 tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt
     8341 tatcacgaca acttccaccc acaaccgcgc tatattccgc tcagccaacc catgccttta
     8401 ctggcaaata cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc
     8461 gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt
     8521 ctctgggggt taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc
     8581 cactgcgagc gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc
     8641 accaacctga tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa
     8701 ctgtgggaag gcagcaccga atgtctggta gtattcccgg atggcgttgg cattctgccg
     8761 tggatggtgc ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat
     8821 tcgctggtgt tgtggccctt ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc
     8881 tttggtttaa tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg
     8941 ggcggcatga aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc
     9001 gttacaccac tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt
     9061 gtcatgcagg taaaccccga cgcccacgaa gagtatcaga gtcggcataa tcccatctgg
     9121 ccggaactgg aagcagtgtt gaaatctcac ggtgtgcata actacgccat ttacctcgac
     9181 aaagcgcgta atctgctgtt tgccatggta gagattgaat ctgaagaacg gtggaatgcg
     9241 gtagccagta ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct
     9301 aacccagata acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg
     9361 cccaatgtgc cggagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac
     9421 atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata
     9481 atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc
     9541 gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca
     9601 tcacttaatc gctccaccat tacaatt
//
LOCUS       DAJTNV010000126         9615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNV010000126 DAJTNV010000000
VERSION     DAJTNV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..620)
                     /gene="pflA"
                     /locus_tag="OO199_003492"
     CDS             complement(<1..620)
                     /gene="pflA"
                     /locus_tag="OO199_003492"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5537403.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELG"
     gene            complement(812..3094)
                     /gene="pflB"
                     /locus_tag="OO199_003493"
     CDS             complement(812..3094)
                     /gene="pflB"
                     /locus_tag="OO199_003493"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5537404.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3149..4006)
                     /gene="focA"
                     /locus_tag="OO199_003494"
     CDS             complement(3149..4006)
                     /gene="focA"
                     /locus_tag="OO199_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5537405.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4412..6172)
                     /locus_tag="OO199_003495"
     CDS             complement(4412..6172)
                     /locus_tag="OO199_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR5537406.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6302..6928
                     /locus_tag="OO199_003496"
                     /pseudo
     CDS             6302..6928
                     /locus_tag="OO199_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7127..8215
                     /gene="serC"
                     /locus_tag="OO199_003497"
     CDS             7127..8215
                     /gene="serC"
                     /locus_tag="OO199_003497"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5537407.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8286..9569
                     /gene="aroA"
                     /locus_tag="OO199_003498"
     CDS             8286..9569
                     /gene="aroA"
                     /locus_tag="OO199_003498"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5537408.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2440 a         2305 c         2418 g         2452 t
ORIGIN      
        1 ttgcccagtt cgtggtaggg gagaagttcg attttctcaa cgttacccat atcacgggta
       61 aattcaccga ggcgatgtgc cgaatcgtca tcgtcagacc agcctgggac aacaacgtag
      121 cggatccaca ccttcacatt tttgttcgcc agatatttag cgaactccag cgtgcggtgg
      181 ttggaaactc caaccagatt ttggtggatc tcgtcgttca tctgtttgag atcgagcatt
      241 accaggtcgg ttacttccag cagttcatca atcaccggat cgtaacgacg aacaaaaccg
      301 ttggtatcca gacaggtatg aatgccttct tttttacagg cgcggaacca gtcacgaaca
      361 aactcagctt gcaggattgc ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc
      421 ataaagtggc gataggtcac cacttccttc atcaaatctt caacggtaac ttctttaccg
      481 ccatgcgtgt cccaggtgtc gcggttatga caatacaggc agcgcatcag gcagccctgg
      541 aaaaaggtga taaagcgaat acctgggccg tctacggttc cacaggattc aaaggagtga
      601 atgcgaccaa taactgacat tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt
      661 ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag tcaccttaaa gtatagatag
      721 ctgacaaaaa aggctctcgt gctaaaaaag gccccacttt cgtggagcct ttattgtacg
      781 ctttttactg tacgatttca gtcaaatcta attacataga ttgagtgaag gtacgagtaa
      841 taacgtcctg ctgctgttct ttagtcagcg agttgaaacg tactgcgtag ccagatacac
      901 ggatggtcag ctgcggatat ttttccgggt tttccatcgc gtcgagcagc atttcacggt
      961 tcatcacgtt aacgttcagg tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac
     1021 catccatcag accagccagg ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg
     1081 gaacgataga gaaggtgtag gagataccat ctttagcgta agcaaacggc agtttagcaa
     1141 cggaagtcag agacgctaca gcacctttct ggtcacgacc gtgcatcggg ttagcacccg
     1201 gtccgaacgg cgcgccagca cgacgaccgt ctggggtgtt accagttttc ttaccataca
     1261 caacgttaga agtgatggtc agaacagact gagtcgggat agcgtcacgg taggtgtgca
     1321 gtttctgaat tttcttcatg aaacgttcta ccaggtcaac agccaggtca tctacacgcg
     1381 gatcattgtt accaaactgc gggtattcgc cttcgatttc gaagtcgata gccagaccgt
     1441 cttcgtcacg aatcggttta actttcgcat atttgattgc agacagggag tcagcagcaa
     1501 cggacagacc agcgatacca cacgccatgg tgcggataac gtcacggtcg tgcagcgcca
     1561 tcagagaggc ttcgtagctg tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga
     1621 tgtactgttt agccagccag tccatgaagt gatccatgcg ctccatcact tcatcgtagc
     1681 tcaggagatc gcctttgatc ggttcagact tcggaccaac ctgcattttc agtttttcgt
     1741 caacgccgcc gttgattgcg tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga
     1801 actgcatttg tttaccaacg atcatcgggc ttacgcagca agcgatagcg tagtcatcgt
     1861 tgttgaagtc cggacgcatc aggtcatcgt tctcatactg cagagaagag gtgtcgatgg
     1921 acactttagc ggcgaatttc ttgaagttca gcggcagttt ttcagaccac agaatggtca
     1981 tgttcggttc cggagacggc cccatagtgt acagggtgtt caggaaacgg aagctgtttt
     2041 tggtaaccag ggtacgacca tcgaggccca taccaccgat agattcggtt gcccagattg
     2101 ggtcgccaga gaacagttca tcgtattccg gagtacgcag gaagcgaacc atacgcagtt
     2161 tcatgaccag gtggtcaacc atttcctgcg cttcttgttc ggtgatcttg ccagctttca
     2221 ggtcacgttc gatgtacaca tccaggaagg tggaggtacg accgaaggac attgcagcac
     2281 cgttctgaga cttaacagca gccaggtagc cgaagtaagt ccactggata gcttcctgag
     2341 cgttggtagc cggaccagag atgtcgtagc cgtatttcgc agccatttct ttcatctgac
     2401 ccagagcgcg gtgctgttca gcgatttctt cgcgcagacg gatagtctgt tccaggttta
     2461 cgccgttttc cagatcagcc tgtagagagg tgaactgagc gtatttgtct ttcatcaggt
     2521 agtcgatacc gtacagcgca acgcgacggt agtcaccgat gatacggcca cggccataag
     2581 catctggcag accggtcaga acaccggatt tacggcaacg caggatgtcc ggagtgtaaa
     2641 cgtcgaacac gccctggttg tgagttttac ggtattcagt gaagattttt ttgatcatcg
     2701 ggtccagttc gcggttgtac gctttgcagg aaccttcgat cattttgata ccaccgaacg
     2761 ggataagagc acgtttcagc ggagcttcag tctgcagacc aacaactttt tccaacgctt
     2821 tattgatgta gccagcgtcg tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa
     2881 ctggcgcgtg agtgcggttt tccagtttaa caccttccat tactttgtcc cacagggtgg
     2941 tggtcgcttc agtagcgcca gccaggaagg actcgtcacc ctcgtacggc gtgtagtttt
     3001 tctgaatgaa gtcacggacg tttacttcat tctgccagtc acctttggta aaaccttccc
     3061 aggctgtggc taacttttca ttaagctcgg acatgtaaca cctaccttct taagtggatt
     3121 ttttatttac tgcgtacttc gacaaccatt aatggtgatc gttttcacgc aggtaaatga
     3181 cccagtatgt caacccaacc aacaaaccac cgccgataat attaccgatc gtaaccggga
     3241 tcaggttatc agtgatgaag ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac
     3301 cgacagcggt ccagaattcc ggagatgcga agtcgcggat tacaataccc atcgggatca
     3361 taaacatgtt tgcgatactg tgctcaaaac cgctggcaac aaacatcgcg accggcagca
     3421 ccataatgaa cgctttgtcc atcaggctgc ggccagaata actcatccat actgccagac
     3481 ataccatcag gtttgccagg ataccaagac agacggcctc aataaaagta tggtgcactt
     3541 tgtggtcggc ggtttgtagg acgtttagtc cccattgacc atttgcggtc atatactcgc
     3601 cggaaagcca cattaaaagt acaaacagca gtgcgccgac caggttgcca aaatagacat
     3661 ttagccagtt tttagccaac tgacaccagg tgatgcgccc actcgcctta gcaacaacaa
     3721 tcaacactgt ggaagtaaag agatcggctc cgcagacaac acaaagaatc agccccagag
     3781 agaagcaaat gccgccaacc agttttgcca tgccgaaggg cattgtgcct gtgccagtgg
     3841 ttgctgtgat atagaagacg aatgcgattg agatgaaaac accggcggta atcgccagat
     3901 agaaagtctt aagcggatgt ttcgttgctt tatagacacc cgcctcttcg gccactttgg
     3961 ccattgcagc aggaagtaaa agatcaaaag ggttgtcagc tttcacacta actctctctt
     4021 tattaagtcg gcgacgagat actaacaaag cattatagat gagaaattga tatagatcat
     4081 atctcgcctg gcttataggc ccgtaactcg catggttttt atgcaaatac ggagtaacta
     4141 tttgattatc caaataaaaa taaattttaa aaattaacaa atgagttgaa ttttttccgc
     4201 atcctccgct aaaacagtta attaaaaggg agcatcaggc gaataaagta acaatatcga
     4261 tcgtatttat taaatacaaa ttaccgatat ttaaccttat aattacaatt attttattaa
     4321 tgcaaatata tgtaaagcgg ggcattaaaa aaacgccccg taatataact cagactaatc
     4381 attaagccta cattgcgtag gctatttgat tttattttgc ccagaatgct gctttggcgc
     4441 gctgcagctt ttcgtaggcc ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc
     4501 tatctaccgg ttgcaggccg taaaacgccg cttcgccgct cattgccgca ctggcggctt
     4561 ctacggcatc tgcgccgtac atgcgaacaa acgcattcag atattgcagc ggctggcgat
     4621 cttcttcctg tgccagtaat aacaacgttt gcaggcagcg ataatagttg gcgcgttccg
     4681 gactaaatac tgatgagtta aactccatcg tccattcggt ccagaccaga gcctgttcca
     4741 gatcgccacc agccagcgcc agcatggctt ttaattcacc gatacgcagg gtgtaccaac
     4801 cgttatccga cccggtcgcc agacccaaca gctcacgcac gcgggtaaag tcatcaaaat
     4861 cttcttcatc cagttgctcg atgaggttca ggtaatcttc tttttcccac tcgctgcctg
     4921 gtagcgaaag aatcgtttca cgtaaatggc tgcccatact gttattcgcg agccacagat
     4981 cttcagccgg ataaatatcg gacatgccag gcacgataat acggcaagca taaacgccca
     5041 gatgctcgta atcggcaata taaacttctt tatcttcttt gttgaagata gccatcagcg
     5101 tagcaaactc ttcttccgtg gtgccggaga aattccagtc cacaaacgga taatcggcat
     5161 cctgcttgaa caggtcccag gagattaaac cgctggaatc gataaagtgc gtttcgaggt
     5221 tggtatgttc agcgacttct tcatcatcga aggttggcgg agtaaacaca tccaaatctt
     5281 tcaggccacg accttgcagc agctcggtca cggtacgttc cagtgctacg ccaaaatcag
     5341 gatgcgcacc gaaagaggca aagcaggtgc cgtttgcagg attaaacagt accacgcaaa
     5401 tcaccggata ctggccgcca agggaaccat catatgcgaa gatcgggaaa ccttctgttt
     5461 ccagtgtttc gatcgcttca accaccgctg ggtaacgcgc cagcacgtct gccgggattt
     5521 ccggcaagct gatgctttca gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg
     5581 acaacccctg aacgcgtgct tcgttgcggg tattgccagc ggacataccg ttagaaacgt
     5641 acaggttacc aatgatattc atcggaatat aaacggtctg attgtcggac tggcgcgtaa
     5701 acggcagacc gcaaatacca cgatcttcgt taccggattg taggtcaatc agcatactgc
     5761 cggtcaattc attctccgga tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg
     5821 gcacatcgtc attttcgctc agtgggaacc atttttcgtt gggataatgc acaaatggac
     5881 cgttggcgat ggtttcgccc agccagaaat cagcaaaaaa gtagttggtt gagagacgct
     5941 cgaaatattc accgagtgca gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg
     6001 taaaacacag tgcgcactct ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat
     6061 tcagccagga ggcctcttca atctgaaagc cgaggtctga aagtttttgc tggaagcgag
     6121 cgatggaatc ttccagagcg gcatctttgc cggggataaa tgtttgcgtc atgaaaatca
     6181 ctttagtcgt acggaaagcg cgcaataata cgggttttat ctcaaaggcg ctatcaccgc
     6241 cgccatgccg atgaactgtt gactatgctt ttagcggata aaccacgtta agagcataaa
     6301 aatgaaggcg ttcgatctcc accgtatggc atttgataaa gtgccttttg atttccttgg
     6361 cgaagttgca ctacgtagtc tttatacctt tgtactggtt ttttgttcct caaaatgacc
     6421 ggaagacgcg gtgtgcgaca gatgtcgctg tttgaagtgt taatcattct cacgctgggt
     6481 tcagcggcgg gagatgtggc gttttatgat gatgtgccga tggtgccggt acttatcgtc
     6541 tttatcactc tggcgttgtt ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa
     6601 actcaataac tccaacatga cggaatttga gttctttatg gagctacgat tgcgtggcgt
     6661 ggagcagctg gggcaggtac gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta
     6721 tttctttgaa gatgacaagg tgaaaccggg tttacttatt ttacccagtg attgtacgca
     6781 gcgttacaaa gtggtgccgg agtcggcgga ctatgcctgc attcgttgta gtgaaatcat
     6841 tcatatgaac gcgggggaaa aacaattatg tccgcgctgt gcaaatccag aatggacgaa
     6901 ggcaagtcgg gcaaaacggg tgacctgaca gtaaaaacat cggctttttg ctaataatcc
     6961 gagagattct tttgtgtgat gcaagccaca tttttgccct caacggtttt actcattgcg
     7021 atgtgtgtca ctgaatgata aaaccgatag ccacaggaat aatgtattac ctgtggtcgc
     7081 aatcgattga ccgcgggtta atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt
     7141 caattttagt tctggtccgg caatgctacc ggcagaggtg cttaaacagg ctcaacagga
     7201 actgcgcgac tggaacggtc ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga
     7261 gttcattcag gttgcagagg aagccgagaa ggattttcgc gatcttctta atgtcccctc
     7321 caactacaag gtattattct gccatggcgg tggtcgcggt cagtttgctg cggtaccgct
     7381 gaatattctc ggtgataaaa ccaccgcaga ttatgttgat gccggttact gggcggcaag
     7441 tgccattaaa gaagcgaaaa aatactgcac gcctaatgtc tttgacgcca aagtgactgt
     7501 tgatggtctg cgcgcggtta agccaatgcg tgaatggcaa ctctctgata atgcggctta
     7561 tatgcattac tgcccgaatg aaaccatcga cggtatcgcc atcgacgaga cgccagactt
     7621 cggcaaagat gtggtggtcg ccgccgactt ctcttcaacc attctttccc gtccgattga
     7681 cgtcagccgt tatggtgtaa tttacgctgg cgcgcagaaa aatatcggcc cggctggcct
     7741 gacaatcgtc atcgttcgtg aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat
     7801 tctggattat tccatcctca acgataacgg ctccatgttt aacacgccgc cgacatttgc
     7861 ctggtatcta tctggtctgg tctttaaatg gctgaaagcg aacggcggtg tagctgaaat
     7921 ggataaaatc aatcagcaaa aagcagaact gctatatggg gtgattgata acagcgattt
     7981 ctaccgcaat gacgtggcga aagctaaccg ttcgcggatg aacgtgccgt tccagttggc
     8041 ggacagtgcg cttgacaaat tgttccttga agagtctttt gctgcaggcc ttcatgcact
     8101 gaaaggacac cgtgtggtcg gcggaatgcg cgcgtctatt tataatgcca tgccgctgga
     8161 aggcgttaaa gcgctgacag acttcatggt tgagttcgaa cgccgtcacg gttaatgccg
     8221 aaattttgct taatccccac agccagcctg tggggttttt atttctgttg tagagagttg
     8281 agttcatgga atccctgacg ttacaaccca tcgctcgtgt cgatggcact attaatctgc
     8341 ccggttccaa gagcgtttct aaccgcgctt tattgcttgc ggcattagca cacggcaaaa
     8401 cagtattaac caatctgctg gatagcgacg acgtgcgcca tatgctgaat gcattaacag
     8461 cgttagggtt aagctatacg ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg
     8521 gcggtccatt acacgcagaa ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa
     8581 tgcgtccgct ggcggcagct ctttgtctgg atagcaatga tattgtgctg accggtgagc
     8641 cgcgtatgaa agagcgcccg attggtcatc tggtggatgc gctgcgcctg ggcggggcga
     8701 agatcactta cctggaacaa gaaaattatc cgccgttgcg tttacagggc ggctttactg
     8761 gcggcaacgt tgacgttgat ggctccgttt ccagccaatt cctcaccgca ctgttaatga
     8821 ctgcgcctct tgcgccggaa gatacggtga ttcgtattaa aggcgatctg gtttctaaac
     8881 cttatatcga catcacactc aatctgatga agacgtttgg tgttgaaatt gaaaatcagc
     8941 actatcaaca atttgtcgta aaaggcgggc agtcttatca gtctccgggt acttatttgg
     9001 tcgaaggcga tgcatcttcg gcttcttact tcctggcagc agcagcaatc aaaggcggca
     9061 ctgtaaaagt gaccggtatt ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg
     9121 tgctggaaaa aatgggcgcg accatttgct ggggcgatga ttatatttcc tgcacgcgtg
     9181 gtgaactgaa cgctattgat atggatatga accatattcc tgatgcggcg atgaccattg
     9241 ccacggcggc gttatttgca aaaggcacca ccacgctgcg caatatctat aactggcgtg
     9301 ttaaagagac cgatcgcctg tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag
     9361 tggaagaggg gcacgattac attcgtatca ctcctccgga aaaactgaac tttgccgaga
     9421 tcgcgacata caatgatcac cggatggcga tgtgtttctc gctggtggcg ttgtcagata
     9481 caccagtgac gattcttgat cccaaatgca cggccaaaac atttccggat tatttcgagc
     9541 agctggcgcg gattagccag gcagcctgaa tgaacaacgg gcaataaata gccaaatctt
     9601 tctttatcaa aacgt
//
LOCUS       DAJTNV010000127         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNV010000127 DAJTNV010000000
VERSION     DAJTNV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..190)
                     /locus_tag="OO199_003499"
     CDS             complement(<1..190)
                     /locus_tag="OO199_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5537409.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO199_003500"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO199_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5537410.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO199_003501"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO199_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5537411.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO199_003502"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO199_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5537412.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO199_003503"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO199_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO199_003504"
     CDS             complement(3262..3381)
                     /locus_tag="OO199_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5537413.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO199_003505"
     CDS             complement(3342..3527)
                     /locus_tag="OO199_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537414.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO199_003506"
     CDS             3628..3801
                     /locus_tag="OO199_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537415.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO199_003507"
     CDS             3803..4147
                     /locus_tag="OO199_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5537416.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO199_003508"
     CDS             4151..4486
                     /locus_tag="OO199_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5537417.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO199_003509"
     CDS             complement(4543..6864)
                     /locus_tag="OO199_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5537418.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO199_003510"
     CDS             complement(6981..7190)
                     /locus_tag="OO199_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537419.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO199_003511"
     CDS             complement(7941..9464)
                     /locus_tag="OO199_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5537420.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTNV010000128         9277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNV010000128 DAJTNV010000000
VERSION     DAJTNV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..1364
                     /locus_tag="OO199_003512"
                     /pseudo
     CDS             1..1364
                     /locus_tag="OO199_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
     gene            1587..2084
                     /gene="ubiC"
                     /locus_tag="OO199_003513"
     CDS             1587..2084
                     /gene="ubiC"
                     /locus_tag="OO199_003513"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5537421.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2097..2969
                     /gene="ubiA"
                     /locus_tag="OO199_003514"
     CDS             2097..2969
                     /gene="ubiA"
                     /locus_tag="OO199_003514"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5537422.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3124..5547)
                     /gene="plsB"
                     /locus_tag="OO199_003515"
     CDS             complement(3124..5547)
                     /gene="plsB"
                     /locus_tag="OO199_003515"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5537423.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5718..6086
                     /gene="dgkA"
                     /locus_tag="OO199_003516"
     CDS             5718..6086
                     /gene="dgkA"
                     /locus_tag="OO199_003516"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5537424.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6196..6804
                     /gene="lexA"
                     /locus_tag="OO199_003517"
     CDS             6196..6804
                     /gene="lexA"
                     /locus_tag="OO199_003517"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5537425.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6877..8202
                     /gene="dinF"
                     /locus_tag="OO199_003518"
     CDS             6877..8202
                     /gene="dinF"
                     /locus_tag="OO199_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5537426.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            8318..8527
                     /locus_tag="OO199_003519"
     CDS             8318..8527
                     /locus_tag="OO199_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5537427.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8569..9084)
                     /gene="zur"
                     /locus_tag="OO199_003520"
     CDS             complement(8569..9084)
                     /gene="zur"
                     /locus_tag="OO199_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5537428.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2229 a         2146 c         2423 g         2479 t
ORIGIN      
        1 ttagtaatga tactataagt gtaaaattct acagaaatga aaaaatagaa tgcgcttgta
       61 attttctgat ggataaagat gcgcaggggt atatcgacct gtctgatttt gatttaacaa
      121 attgtcattt taaaggtgac gttatttcta aggtgtcttt tttatcatca aatctacaac
      181 atgtaacttt cgaatgtaaa gaaattgaga attgcaattt tactaaggca acagttgata
      241 atgtcatatt taaatgtcga cgtttacaca atgtgatttt tctcaaaacg agtggcgaat
      301 gtgttgattt tagccaaaat attcttgata cagttgactt ctcgcagagt caacttggtc
      361 atagtaattt tcgcgaatgt cagattagaa attcaaactt cgataattgt tatctttacg
      421 cttcgcactt caccagagca gagtttctgt ctgccaaaga aatatcattt attaaatcga
      481 atttgacagc tgttatgttt gatcatgtgc gaatgtcgac agggaatttt taagattgca
      541 ttacagaaca attggaatta actattgatt attcagatat atttgggaat gaagatctcg
      601 atggttatat caataacatt ataaaaatga ttgatacatt gccagataat gcaatgatat
      661 tgaaatccgt tctggcagta aaactggtga tgcaattaaa aattctcaat attgttaata
      721 aaaactttat taagaatatg aagaaaacat ttagccattg tccttatata aaagatccaa
      781 ttatacgtag ttatatacat tctggggaag atgacaagtt cgataatttt atgcgtcaac
      841 atcgattcag taaggtgaat ttcgataccc aacagatgat ccattttatt aacagattta
      901 atatgaataa agggctggtt gataaaaata acaatttttt tatccaactt atcgatcagg
      961 ccttacgatc aacggatgat atgatcaaag caaatgcctg gtatctttat aaagagtgga
     1021 ttcgtagtga tgatgtttca cctatattta tagaaactga agaaaagtta agaaccttta
     1081 acactaataa attaacacga aacgataata tatttatcct gttctcctct gtcgatgatg
     1141 ggcctgttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
     1201 ccaattggaa ttccacgtgt atctataaat ccagacataa gatgttgcct attaatctta
     1261 ctcaggaaac acttttcagc tccaaatctc atggtaaata tgcgcttttc ccaattttta
     1321 ctgcgagttg gcgagctact cgtataaaga ataaaggtat ttaagtaaag gaaaacatca
     1381 ccgttcctag catcatgggc ggtgatgccc ctatggttgc cctcgccagc acgggcatcg
     1441 gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt ttttgttgcg
     1501 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
     1561 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
     1621 tgcgctattt taaagagatc cctgccctgg atccgcaact gctcgactgg ctgttgctgg
     1681 aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagtgtg acgatgatcc
     1741 gcgaggggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt
     1801 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
     1861 gtaccgtcgt tcctgtatca acattaagcg ggccggaact agcgttacaa aaattgggta
     1921 aaacgccgtt aggacgctat ctgttcacat catcgacact aacccgggat tttatcgaga
     1981 taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc
     2041 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
     2101 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
     2161 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
     2221 gcgttcctca gctctggatc atggcggtgt ttgtcgcggg tgtctggctg atgcgcgctg
     2281 ctggttgcgt ggtgaatgat tatgctgatc gcaagtttga tggtcatgtt aagcgcacgg
     2341 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag
     2401 tgctggtact gatttcgttt ttactggttc tgacgctgaa tacgatgacc attctgttgt
     2461 cgattgccgc tctggcattg gcgtgggtgt acccgtttat gaagcggtat acccatctcc
     2521 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gccgctgtga
     2581 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
     2641 cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt ggcattaaat
     2701 ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
     2761 tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt
     2821 caattgtggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
     2881 gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctgtttttag
     2941 gactggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
     3001 ttcttctggg ttgcctgatg cgttgcgctt ctcaggccta cccggtgaat cgcaatttat
     3061 tgaatttgca ggtcatggta ggccggataa ggcgttttcg ccgcatccgg caattctctc
     3121 tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
     3181 ctccgccagc aactgataaa ccttcatcgt ttctgctggt tcggcatcgc cgctatcgct
     3241 gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct tgtcgaagaa
     3301 ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcaacggtgc ggctctcttt
     3361 ctccagcgta ccgcggttga tcgacgggtt ggcactcaac aaccagaagg tgatggcata
     3421 acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agggtgcgag aatgcgccgg
     3481 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
     3541 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
     3601 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
     3661 ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg
     3721 atagtaggtc atcagcaccg cttgctcgcg tggcagaatg atgatgtcgc cgatagtgtc
     3781 tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
     3841 tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca ggtagcagtt
     3901 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
     3961 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
     4021 agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga tgggatcgat
     4081 agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
     4141 gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
     4201 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
     4261 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
     4321 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtgcg
     4381 ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca gttcgccgag
     4441 atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc gaataaagaa
     4501 cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccggcggc
     4561 gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca ggtagtccat
     4621 gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca gctggcgaac
     4681 gcgctcggcg ttatggacgt tgatgccctg ataaaatcgg ttccaggtga agcccagaat
     4741 gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc
     4801 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
     4861 cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcag
     4921 acgtgggcct acggcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
     4981 tttctgagcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgacactga
     5041 cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa atttctgtac
     5101 gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc
     5161 aaacatcacc gatactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc
     5221 gagatagtcg tggaaaagct taatagactc ttctttcggc gtgtaatagg tgaatacacg
     5281 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
     5341 taacgggtca ggcaaatcat gtgccagaca ctgggcgcgc aacgtcagca aatctgcttt
     5401 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
     5461 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
     5521 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
     5581 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
     5641 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
     5701 gtaaaaaggt tctttcaatg gccaataata caactggatt cacccgaatt atcaaagctg
     5761 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
     5821 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcaatta
     5881 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
     5941 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcaa
     6001 aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt acctggtgca
     6061 tcctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc tggtttattg
     6121 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagtata actgtatata
     6181 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     6241 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     6301 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt
     6361 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     6421 ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa
     6481 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
     6541 agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc agtgcataaa
     6601 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
     6661 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     6721 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt
     6781 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgct tggcgtcgcg
     6841 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
     6901 aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg
     6961 ggactggtcg atacggcggt aattggtcat cttgatagtc cggtttattt gggcggcgtg
     7021 gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc
     7081 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
     7141 ctggtgcaac cgttgctgtt ggcgttgggg gccggggcgt taattgcgct gttgcgtacg
     7201 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct ggaacaggcg
     7261 cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa tctggtatta
     7321 ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
     7381 aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat gaacgtgcag
     7441 ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg tctgctaatg
     7501 gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga
     7561 ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg
     7621 caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga cattatcgct
     7681 gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgcttga tggttttgcc
     7741 tacgcggttg aagcgcactc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg
     7801 gatgtatggc gggcagcgtg ccgccagtcg gggattgtag cgttactgtt ttcggtggtt
     7861 tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag
     7921 ctggctgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt ctggtgttat
     7981 ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg
     8041 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg taatcatggt
     8101 ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg
     8161 cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa aattctgaat
     8221 aaattatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
     8281 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
     8341 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
     8401 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
     8461 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
     8521 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
     8581 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
     8641 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
     8701 catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact cttctttcac
     8761 cgcgccgcag cgatcgcaaa taaacatggc tgacgtatgc gtgggctggt cgaacagatg
     8821 acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
     8881 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagtaaatc
     8941 aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
     9001 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa
     9061 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
     9121 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgattttct
     9181 gttttgtagg taaggtgttg tgttgccttg tcgtaccatt atcaacacga taataattaa
     9241 taattcattt ttaaataaat tgcttcaagt taactcg
//
LOCUS       DAJTNV010000129         9244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNV010000129 DAJTNV010000000
VERSION     DAJTNV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO199_003521"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO199_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5537429.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO199_003522"
     CDS             1828..3147
                     /locus_tag="OO199_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5537430.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO199_003523"
     CDS             3445..3486
                     /locus_tag="OO199_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537431.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO199_003524"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO199_003524"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5537432.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO199_003525"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO199_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO199_003526"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO199_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO199_003527"
     CDS             5815..6711
                     /locus_tag="OO199_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5537433.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OO199_003528"
     CDS             6708..7694
                     /locus_tag="OO199_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5537434.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OO199_003529"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OO199_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO199_003530"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OO199_003530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5537435.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2258 c         2318 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atat
//
LOCUS       DAJTNV010000130         9232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNV010000130 DAJTNV010000000
VERSION     DAJTNV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(4..210)
                     /locus_tag="OO199_003531"
     CDS             complement(4..210)
                     /locus_tag="OO199_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537436.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSVSGTESSATRQH"
     gene            complement(427..960)
                     /locus_tag="OO199_003532"
     CDS             complement(427..960)
                     /locus_tag="OO199_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5537437.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1026..1655)
                     /locus_tag="OO199_003533"
     CDS             complement(1026..1655)
                     /locus_tag="OO199_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537438.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1659..1874)
                     /locus_tag="OO199_003534"
     CDS             complement(1659..1874)
                     /locus_tag="OO199_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5537439.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2167..2241)
                     /locus_tag="OO199_003535"
     tRNA            complement(2167..2241)
                     /locus_tag="OO199_003535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2206..2208),aa:Gly,seq:tcc)
     gene            complement(2245..2320)
                     /locus_tag="OO199_003536"
     tRNA            complement(2245..2320)
                     /locus_tag="OO199_003536"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2285..2287),aa:Thr,seq:tgt)
     gene            complement(2323..2399)
                     /locus_tag="OO199_003537"
     tRNA            complement(2323..2399)
                     /locus_tag="OO199_003537"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2363..2365),aa:Arg,seq:tct)
     gene            complement(2401..2477)
                     /locus_tag="OO199_003538"
     tRNA            complement(2401..2477)
                     /locus_tag="OO199_003538"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2441..2443),aa:Ile,seq:cat)
     gene            complement(2677..3057)
                     /locus_tag="OO199_003539"
     CDS             complement(2677..3057)
                     /locus_tag="OO199_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5537440.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3050..3250)
                     /locus_tag="OO199_003540"
     CDS             complement(3050..3250)
                     /locus_tag="OO199_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5537441.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3345..3602)
                     /locus_tag="OO199_003541"
     CDS             complement(3345..3602)
                     /locus_tag="OO199_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537442.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(3599..4990)
                     /locus_tag="OO199_003542"
     CDS             complement(3599..4990)
                     /locus_tag="OO199_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5537443.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(4987..5865)
                     /locus_tag="OO199_003543"
     CDS             complement(4987..5865)
                     /locus_tag="OO199_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5537444.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(5876..6784)
                     /locus_tag="OO199_003544"
     CDS             complement(5876..6784)
                     /locus_tag="OO199_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5537445.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(6771..7004)
                     /locus_tag="OO199_003545"
     CDS             complement(6771..7004)
                     /locus_tag="OO199_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537446.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7001..8017)
                     /locus_tag="OO199_003546"
                     /pseudo
     CDS             complement(7001..8017)
                     /locus_tag="OO199_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            7949..8212
                     /locus_tag="OO199_003547"
     CDS             7949..8212
                     /locus_tag="OO199_003547"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5537447.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8202..8510
                     /locus_tag="OO199_003548"
     CDS             8202..8510
                     /locus_tag="OO199_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5537448.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8552..8728)
                     /locus_tag="OO199_003549"
     CDS             complement(8552..8728)
                     /locus_tag="OO199_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5537449.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(8725..9135)
                     /locus_tag="OO199_003550"
     CDS             complement(8725..9135)
                     /locus_tag="OO199_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5537450.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
BASE COUNT         2240 a         2481 c         2131 g         2380 t
ORIGIN      
        1 ttctcaatgc tgtctggtcg ctgatgattc cgtcccggat accgagaacg tttcgtccag
       61 caactggaga gagttcgacg gtggcatcat tgcccatgcc ggaggcgctg gaggtttcgg
      121 ctgaggatgg cacagggcat tttcctttga cgagcacccg accaccatta tcaagcttgc
      181 gccgaagagc atcattttca gctttcgcat cagctaactc cttcgtgtat ttagcatcga
      241 gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat ggttgcgttc gccagcttca
      301 gttctccggc gtttttgtcg cgctgctctt tgtaggtaat ggcgttatca cggtaatgat
      361 taacagccca tgacaggcag acgatgatgc agataaccag agcggagata atcgcggtga
      421 ctctgctcat tgatctatcc cccaacaggc taatgcgctt tcctggtcac gacgaataac
      481 ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc ccaacatctt ttatccacca
      541 gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc agccgcttat aaaacgtcga
      601 cgggaaacac ttaccggggc caatgttata ggggcaaaat gacgctatac ccgctttctg
      661 gggttcggtc agaggtactt taatattgcg ctccacccat gccagcgcct tatcacgttc
      721 aatagcgtta acctggtcac atttttcctt cgacagcttc attcccggta tgacaggctt
      781 accatccacc attgtggcac cacgacagat ggtccatatg ccagaaccat cacggtatgc
      841 cgttgtgtga ttaccctctt tttcgttcag aaactggtca agtatctggg gagcagacgc
      901 gcctgcggca atcagcgcca gaacagcagc cgacaggccg tatttgattt ttgcgctcat
      961 ggatatttat caggatttat cggcaacaaa taacgagcca acttatatac gccttttaag
     1021 ataagtcagc cccggctgga tccagtcatc aggctctttc ttaaaggggg agtattgaaa
     1081 tcacgaagaa gagcctcccg caccgcagca tccatgtctg caccactggc aagtgcttca
     1141 atctctgccg ccacctgcaa ataccccatg caacggccaa tgcgtttcat aactctggtg
     1201 ttttcgtctt tcaggcatct gatagcgaga ttaataattt tcaggcagtg atgatgcgat
     1261 gcccggacag gttttccgtt ctcataaacg acgaactcac cctcttcaca ggaaaccctg
     1321 aaaggccgat ggggttcctg gtctttatcc ggtgcagacg caataaaatc acttttatgc
     1381 tcttcctctc cggcaccgtt aaccgtaatg acgtactcag gccgcaggta agccgcctct
     1441 ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa acttcataaa agtctcgtga
     1501 gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct catcgcggcg tttgttttca
     1561 tcttcagaac tcaccaacgc cgacaatttt ttattaagct cagtgatttc attttccagg
     1621 tgattgaatc gctgattcat ttcttcatgc ttcattattt actctccccg tgcagcctta
     1681 cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat aagtcagaag ccccagaagc
     1741 agacttccca gcacaccaat cgcagcccac tgtgatggcc tgacctgatc caaccactgc
     1801 aaaaaccagt agccggcact gccggcggag gtgccgtagg caatgcccgt tgaaattttg
     1861 tccatggatt tcatagcctc acctccgcaa ataacggatg gcgtagtttt acactgagaa
     1921 atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg aaacgatata tacagtaagg
     1981 aaagcactct atccaacaaa ccacccacag ttaatcggaa taaaagcaga gtgcttatga
     2041 atgatcgcac gcccgaaggt tagtatttct gcacagcaat tttgcaaaaa aaggcgatca
     2101 ttcataactt aaacgtcttt cagtcactcc gggatttccc atcatcgcag actgaaagac
     2161 tctgactgga gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa
     2221 tagccattat acgatgcccg catatggtgc cgactaccgg aatcgaactg gtgacctact
     2281 gattacaagt cagttgctct gcctgctgag ctaagtcggc gctggcccac caccgaggac
     2341 tcgaacctcg gaccgtcaac ttagaaggtt gatgctctat ccggatgagc tagtggtggt
     2401 tggtggccct tgctggactt gaaccagcga cctggcgatt atgagtcgct cgctctgacc
     2461 aactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca
     2521 tctgaccacc agcgtgttta acgtcctgta ccgtttttca ggcataaaaa acccgcccgg
     2581 aggcgggttt aagctgtgtg gcgaagcaac cactcttaac agaatatccc gttttttacg
     2641 tacgtaaaat attttctaga aagtcgcccc ttaccatcag ggatgttcaa tatatttgtc
     2701 catttctaac cggacaccca acatcatcaa cattccttcc accaccccct cggctttttg
     2761 tagcttcttt cctatataac catctgagca tccatgcttc cgcgccagcg tcatgaatgt
     2821 catcccaaac acgtagtagt caaccagcaa gtcatgtaga tcgttgttgt tccggttaag
     2881 gcgggccata cacccacata taaccatcgc gtcatcgtca cagcattgcg ggcgggattt
     2941 tacttttgaa gggattaatc ccttaaaacc ggcagcaatg gacgaccagg tcacatcctc
     3001 atgattatta gccacccacg cgccccagcg ctcaagaacc tgctgaatat cacgcatcac
     3061 tgtctttacc cctgtcccat ccacgatgaa caatcagaac accatcaaca atggcgtgcc
     3121 ctttgccctc tttatcttca gcatattttc ttaccgtggc gcgattacag ttcagcattc
     3181 gtgaaacttc ggtcatatta cctcgtgtct ggataagcag ttccggtatc gtttgaattt
     3241 tgacgctcat caaatactct ccggttcggt gatttttatc ccgggctttc caccagggac
     3301 ataatgatcg cgcacaagcc agggcaaaac aaactcatgg cgcatcagcg cagcgcctcc
     3361 tgcaccagtt tttcaaactt tccgactctg gtttccagct ctgccacaca gtccaccagc
     3421 tcatctactg ctttctgtgc gcgatgcttc gcctgcatca gttcccgaag cgcgggtacc
     3481 atatctttac ggatagcatc ttttgttatg cccgtttttt cgagttgttc agcatgacgc
     3541 agcatttcct gcgcgtgttt acgcaattgt tcaggggtaa aagtcattgt ctggttgttc
     3601 aaaagaaacg ctccatctta ctgctgtcag ttcgtttatt actgtatctg cgcggattgc
     3661 cgggcttcat gggagtggaa agcacccgtg cactttcctg gtccacaggc agaaaatgtc
     3721 cgttataaaa acgccggtaa atcgttccca gagaaccgtt acgttgtttc gtgatattga
     3781 tttctgcgat gcccctggcc tgcgtatccg ggttgtacac ttcatccctg taaagcatca
     3841 gaatgatgtc tgcatccgcc tctatttctc cggaattttt caggtctgag ttcatgggac
     3901 gtttattggg tctggactcc acaccgcggg agagctggct cagcgcaatc aacggaaaac
     3961 caccggattt tgccaggcct ttaagcccct ttgagatttc acccacggca aggtcatgac
     4021 gccccgtggt tcgggttttt atcagcccga gataatcaac caccaccagc gccgtttccg
     4081 gatatttaat caggtggtgt ttcgttgttg cgcatatctc atcaatggcc aggttcgcct
     4141 ggtccaccat ccagatattg cgcccggtca tccgccccac cccttgtgag aaacgcgtcc
     4201 agtcttcatc ttcaaagtga gccacagatt tcaggcgtga tactggcatc cctccagccg
     4261 cagacaccat acgttcacca atctggatgt tcgccatctc catggtgaac agaagcacac
     4321 catgcccctg ctcagtcacc ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg
     4381 aaggacgggc ggcaatgaat accaggtctc cgggctccat accgcctgtt tttgcgtcca
     4441 gttcatcaat accggtcatc aacgtcctgg atttctccag cccctgattc cggcattcaa
     4501 cacgctcaac cacttccgga agcacatcat caatatgtac cggctgaatg acgccctttc
     4561 ctgtcgacag tgtgaccatc atgttctgcg catccttcag ggcatcttca gctgcttcac
     4621 aggtatgcgc atcacgtaat ttctgcagcg cctcattcag tgttttttct gcatcgcgca
     4681 gtgcggcatt gcgccgcaac gctgcaacat agtgctccag tgaagacttc acccaggttt
     4741 tacgcccggt atcagtaatc accggggcaa gttccggcat ctcattacac aacagcacgg
     4801 ggtcaatcac tcctgaaaca cgggcctgtc tgcagatgcc tgtatagata tcctgatacg
     4861 ctcgtacaga aaaaacgtcc gctggcagtg tggccagaat atccatcact tcaggatctg
     4921 ctccgcgcag aaagaacgcg ccgatgacag cgccttcaag gtcatcgtta cgccatactg
     4981 gagttgtcat gcagccacac ctctgatacg agaacggtaa ctgggccagt taaacgacaa
     5041 ccagttgcgt cccccgtctg tgatcctgtc ggcaattcgg ggactgatga acgcccacaa
     5101 ctcttccggt gagaggttac tgataagaat ggtgggcaag atactttcgt acctggcgtt
     5161 gataatttcc tgcaaaatag ccatttcagc cgcgctgcca aactgaacgc caacttcgtc
     5221 gatgatcagc aaatccattg acgcataacg ctcaataact tcatccgctg ttttttcgct
     5281 gtcattccgc cagcaatttt tcacagcacg ggtaaggcgc atcacgtcgg tgatctccac
     5341 actggccaga tagttacgga tgatgtgttt tgccatagcc acagccagat gattttttcc
     5401 ggtaccacaa ctgccggtca taacaagact ggtaccgttc tccagcatat ctggccagtt
     5461 ctccgcatag cggcgacagg ccgcaagatt tctggctgcg ccaggattaa cctccagata
     5521 attatcaaac tcgcagtccc gaaaacgcag ggcaattccg gcgttatcag tcagctcttc
     5581 cgccttgatg gacgacagct ccatggtcaa atcgttggcc tcagcgatta agcagtcagg
     5641 gcagcatgaa atttcttctc tgtcctcgcc attacgatcg ctccacacca gtatatgcgt
     5701 gtgatattcg ccatgttttt cgcaatatcc gcgaccttca cgcatcaggc aggaacgata
     5761 aggccatggc ttttcgccct tctgagcaaa tgcaatctct gcccgtaact catccattcg
     5821 cgcctgtagt cttttttgtt gttcacgcag gttaaacgtc atcatcgctg tcacctcaga
     5881 atgtcagttt gtcactggat ttaccgaatt tgtcagacat ggctcccagg ccagccagga
     5941 catcgacctg tcgctgtcgc ccacctctgg gagcggctgg ctgttgccag aagtcttcga
     6001 agtgacgatc gggtccaaag aacgtcgcag cctgcttcac gaactgtgtg ccggtatttc
     6061 ctgtggcacg tacccaggca gcatagcgtt tcacgccatc aagcatggct tcaggtttta
     6121 ttccctccct gagccgggct ttccaggctt tgaaggctgc ggattttgag ttaccaccag
     6181 cacgttttgg atattcctgc caggcctgtt caaattccgg tgaatattcc tgtcgggaag
     6241 aacgcgctgg tgcagacgcg tcagcggatg cgccaatagt gtttttactc tctgtagtat
     6301 tctctgaagt aatctctgtt gtattctctg taagatcgaa attggttttc ccttctccgc
     6361 ggcgaggggt ttcccgtgtc cgcggtgaag gctttccctc ctccgcgaaa ttgggtttta
     6421 cagtttcccg aaaacgggtt tccccatttc gggaaaactg attgttttca ttgataattt
     6481 cattaaggcg ctcacaatct atacggtaga acattttgtg ctcaagacgc ttgttggttt
     6541 caaccaaaat gcctctggac acaagatgct tacgcgctac agcctgttgt tcaaatgtaa
     6601 gtccggtttc gtgttgtatc tcttcacgcg ttttatgtac gccttccgct gcatgtgctt
     6661 tatcctgcca gtaaaaaatc tgaccaaaga aaataacagc gtgcggactt cccatgtatt
     6721 taacgagccc agggtaataa gcaaccggat gtccaaaatc gagcagaaga tcagacggac
     6781 gcatagccac ctcccaggcg tttaaacatt tttccgggct gaaacgccac cagcggataa
     6841 ctcagggtat gagtacgtcc ctgaacctgg cagacaacct tctggctttc ggtactgacc
     6901 aggcaaaccc gcagaacgta gccgttgctg gtggtgaacc actgccccac acgggggcaa
     6961 tggttgtatc ggtgatacag gaaattaacg atgtggcgga tcatgggcgc acctccttgt
     7021 cagaaccatt cagcctggaa tcaacaagtg cagcaccaaa aacagcatca cctacacggt
     7081 cgtacagttt gccagccagc ggagattcaa cggccttaag cattgggtaa agctggcttg
     7141 tccagatttg atggatttca cgcaaatgca ggtatacgcc tctggcgttt tgtgcgacag
     7201 atgacatgtc agccgcacca gctcctgata agctcctctc catctggtta aaggcattga
     7261 tgtatgcctc tttgaaccgg gcggcacgtt taccagtgaa gcccatagca aggaaggcga
     7321 agccgccgcg ggtaatttgg tagcagggaa gtttgcgagt accgccgttg ggctggcgta
     7381 ccaaaattga tgtctccgca aaattgcggg cacaaaactc tggagaacaa tccaaaatgc
     7441 ggatcttttt cagaacatcg tcatgacgtt tagagaagaa gttggcaaca gccagggatg
     7501 aagtaacagc ctaaccatca acgatggcaa tttcaggttg agagagggtt gggagagtag
     7561 tcatggtgac agcccctatg ttgaattcaa agaactcacc acatgggacg ccaatcacag
     7621 aggtggtgag acgtacaggg ttggcgtaac cggtcaacat agaacccggc gcatcttgcg
     7681 atgtccctgc acgccccacc ataatttggg cgtagcaatg ctcatgacac gaaaaaaccg
     7741 catgagcgcg gttatgctct atattgaatt tcaggacgcc aatcccggcc cccgctttat
     7801 aaggtgcaga gacagtgtaa cgtcccgaaa ttgcagaatc aatatattgt gcagatataa
     7861 cgattttgtt atgttttgct gtatgaacta cactatcgaa tactacagtg aagaggtccg
     7921 actggaggtc gatcagcttc cattgagtat gcgtgccaga taccagcatc ttgttgaacg
     7981 catgaaggta tacggcagca atctcggaga acctcacacc agcgcctttg gtgacgggct
     8041 tttcgaactc agaattaaag gcaacgatgg gatcgcgcgt gttttttact gcactctgac
     8101 agggaaacgc atcatcatgc tgcatagttt tgtaaagaaa gcgcagaaaa caccgccagc
     8161 cgaacgcaag aaagctgaaa ccagaatgaa ggaggttaag catgactggt aaacgcactc
     8221 tccccaccat gacacacgac gaaatggcag ccaaatggat ggaagacccg gcttttaaag
     8281 cagaatacga cgctatcgct gacgaattcg cactgcttga tgaaatgctg gcagcacgca
     8341 aagaagctgg cttaactcag gctgaagttg ccgagcgaat gggaacaaaa gcgaccgcga
     8401 tcaccagaat ggaaagtaat ctcgcatcag gtatcagcgg cccatcattt gccacactaa
     8461 aaaaattcgc ccgcgctact ggaaaaaaac tccagatccg cttcgtttaa ccacaccgcg
     8521 ccgtcattct ggcggcgcac cggataaata gtcatttcct cgcacgatgt cttagccacc
     8581 ggatatccca caggtgagtc gtgtagttga aggtttttac gtcagattct tttgggattg
     8641 gcttgcgttt atttctggag cgtttcgttg gaaggtattt gcagttttcg cagatgatgt
     8701 cggtgaaact tcgtcgatgt cgcctcattc atacctcctg tcggtaaatc tgacaccctg
     8761 accaatagcc caggctgttg tgtactcgat cagacttgcc atgcgcttca cgctcatctg
     8821 cgcgctgctt tcgcgaatgt tgacgcattc tccttcaagg ccgggcaaaa catcagcttc
     8881 ctgttttgtt gccactgcat gaccgctgat caacaaaacc ttccattgtt ctggttttaa
     8941 ccatttgccg caccattgaa cctgacgagc gatatccgcc agcatcgcgt gaaattttgc
     9001 gttctggtca agatttcgct tgtagtcagt aatgcggatg gtgactggct tgtctttatc
     9061 gagaggagtt gagaggatgg catttattgc ggcttgctgt tgttgcttag ttcggaggaa
     9121 gattgtttgc ttcatcgaaa attcttctct ttaattccag cggctctgat agctttcatt
     9181 actgcaatta ccgttttgtc cttcccatcc tcatgcccca tcgcataagc ac
//
LOCUS       DAJTNV010000131         9014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNV010000131 DAJTNV010000000
VERSION     DAJTNV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO199_003551"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO199_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5537451.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO199_003552"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO199_003552"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5537452.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO199_003553"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO199_003553"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5537453.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO199_003554"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO199_003554"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5537454.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OO199_003555"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OO199_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5537455.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OO199_003556"
     CDS             5545..6018
                     /locus_tag="OO199_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5537456.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OO199_003557"
     CDS             6047..6589
                     /locus_tag="OO199_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5537457.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OO199_003558"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OO199_003558"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5537458.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OO199_003559"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OO199_003559"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5537459.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OO199_003560"
     CDS             7986..8243
                     /locus_tag="OO199_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5537460.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OO199_003561"
     CDS             complement(8219..8773)
                     /locus_tag="OO199_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5537461.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2322 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAJTNV010000132         8898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNV010000132 DAJTNV010000000
VERSION     DAJTNV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..2887
                     /locus_tag="OO199_003562"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO199_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO199_003563"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO199_003563"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5537462.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO199_003564"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO199_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO199_003565"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO199_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OO199_003566"
     CDS             complement(7280..8041)
                     /locus_tag="OO199_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5537463.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OO199_003567"
     CDS             complement(8038..8697)
                     /locus_tag="OO199_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5537464.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2175 a         2357 c         2318 g         2048 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctaccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAJTNV010000133         8861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNV010000133 DAJTNV010000000
VERSION     DAJTNV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..2172
                     /gene="espL4"
                     /locus_tag="OO199_003568"
                     /pseudo
     CDS             1..2172
                     /gene="espL4"
                     /locus_tag="OO199_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2141..3878)
                     /gene="aceK"
                     /locus_tag="OO199_003569"
                     /pseudo
     CDS             complement(2141..3878)
                     /gene="aceK"
                     /locus_tag="OO199_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4062..5365)
                     /gene="aceA"
                     /locus_tag="OO199_003570"
                     /pseudo
     CDS             complement(4062..5365)
                     /gene="aceA"
                     /locus_tag="OO199_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5395..6996)
                     /gene="aceB"
                     /locus_tag="OO199_003571"
                     /pseudo
     CDS             complement(5395..6996)
                     /gene="aceB"
                     /locus_tag="OO199_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7265..8194)
                     /gene="metA"
                     /locus_tag="OO199_003572"
     CDS             complement(7265..8194)
                     /gene="metA"
                     /locus_tag="OO199_003572"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5537465.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8351..8794
                     /locus_tag="OO199_003573"
     CDS             8351..8794
                     /locus_tag="OO199_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5537466.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2307 a         2157 c         2151 g         2246 t
ORIGIN      
        1 ttcctcgtag ttctttctct gcaaatatta ataatacagc ccagacaaat gaacaccaaa
       61 ccctgagtga attgttttat aaagaactcg aggataaatt ttctggcaag gagctggcga
      121 cgcctctatt aaaaagcttc tcagagaact gtagacaaaa tgttcgtcat atctttagca
      181 acaaggattt tgtcattaaa ttttccacgt ccgtcttaca agctgataag aaagaaatta
      241 cgataattaa taaaaacgaa aacacgacac tcactcaaac cattgctaca atatttgaag
      301 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
      361 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
      421 tcccggggcg ccccaaaacc gtatagtata ccgacacatt gctgcacaat atattaatga
      481 tatttatcag aatgttgatt acaaacccca tcaagatgat tactcttcag ctgaaaaatt
      541 tctcactcac ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc
      601 tgaggggcgt tgcgttgctg cctgcggtga tttcgggcta gttatgaaag catattttga
      661 caagatggaa tcaaatggca tcagtgttat ggcagccata ttactggtgg ataaccatgc
      721 tttgacggtc cggctaagaa taaagaacac aactgaagga tgtacccatt acgtggtttc
      781 ggtttatgat cctaatgtaa ctaacgataa aataagaatt atgagcgaaa gcaaagagga
      841 tattaaacac tattctctga tggattttat gaatgtagat tatagcctcc tgaaatggtc
      901 aaatgatcat gttattaacc aatctgttgc aataattcca gcacttccga aagaacagct
      961 attgatgtta aaaggaactg tggatgaaat aacccctcca ttatcaccag caacgatgaa
     1021 tttgctaatg gcaattggtc agaatcacca actcacgcaa ctgatgattc agctccagaa
     1081 aatgccagaa ctacatagaa cagaaatgtt gactgcctat aatagtatta acctccccgg
     1141 tttatatttg gctataaatt atggcaatgc ggatatcgtt gagactattt tcaattcatt
     1201 gtcagaaaca ggatatgaag ggttactctc gaaaaaaaat ctcatgcata ttctggaggc
     1261 aaaagataaa aatggttttt ctggattatt tttagcgata tcacgtaagg ataaaaatgt
     1321 tgtaacctcg attctgaacg tcttacctaa actggccaca acacatcatt tagataatga
     1381 acaggtgtat aaattcctga gtgccaaaaa tagaacgtcc agccatgttt tataccatgt
     1441 tatggcgaat ggtgatgccg acatgctgaa aatttttttg gtcgcgttac ctttgttaat
     1501 tcgcacatgt catttgacta aagaacaggt actcgatctc ctgaaggcaa aggattttta
     1561 tggttgccca agactatacc tggcgatgca aaatggacat agcgatatcg tgaaagttat
     1621 tctcgaagca ttgccctgtc tggcccagga aattaacatt tcagcttccg atattgtaga
     1681 tcttctgacc gctaaaagtc ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg
     1741 acatatgaac gttattaata ctatttttaa cgcattaccc actcttttta atacgtttaa
     1801 attcgataaa aaaatatgaa gcccctcctc ctggcaaata attctaatga atatcccggt
     1861 ttgttttcag cgatacagca taaacaacaa aacgttgtag agacggttta tcttgcttta
     1921 tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt ttggcaacac
     1981 aaagcgccac aaaaatactc tgcctttgag ttggcttttg aatttggtca ccgggttatt
     2041 gctgaattaa tccttaatac attaaataag atggctgaaa gctttggctt tacggataac
     2101 cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc atctccccat
     2161 accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca tgcccttcac
     2221 gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg tgcatctctt
     2281 caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct tccgggaaaa
     2341 catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg
     2401 gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg taatcataaa
     2461 aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa
     2521 tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact
     2581 gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc tcgattatag
     2641 agatggcgaa ttacaatctg ctcaccaaga tcggtgattt tttccgctgc ttcctggagc
     2701 agtaattcca ttaataccgg ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc
     2761 tgggtgtccg ccattcggcc cacacggtcg tgctctttca ccagttgata gcaggcacga
     2821 acgtgagcgg cagacatctc tttctgcggc gcgaacttgt ctttgatgac tttgaatacc
     2881 cgatcaaagc ccggcagcgt aaacaccaac atcaccattc cacgaatacc cggcgcttca
     2941 atgaactgct cattacagcc ctgtagataa acgagatatt cgcggtagct ttcggttttg
     3001 gcgtgcttct ggcagccgat agccatatac aattcagcag tggttttacc tggcagaatt
     3061 tcccgtagcc actcgaccag cgctgcgggc agcggtgcat aaaccataaa ataagaacgc
     3121 gcaaagccaa aaacaatgct cgcttcggcg gtcgtcgtca ggcaggtatc aataaataac
     3181 tcgccgtcgt ccgtctggtg gatcggcagt aagaagggca acgtgccgga aggcgtgatc
     3241 agtttgccca ccagccaggc ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa
     3301 tgactttccg cgaggttgtc tgtccccagc gtttccgtca gatggcgaat gatgtaatga
     3361 atgtcacggc ttttattctg ccagcgcagg cgcagcggca ggtcgctgat aacgcgcatc
     3421 agtagagatt cccagccgtg atcggggtga aagtctttcg ccagcgggcg gggaatggta
     3481 cgaaagcggc gctctggctg agagctaaag ataaaaagcc gctcgggagt aagcgagcgg
     3541 tggtcaaata accgacagta cacggagtta aaaaagctct ccgcaatctc gaagcgcggg
     3601 taatccggca acagccgcgt gtaatgctct ttaacgcgca gtaaaaatgc cgcgtccgta
     3661 ctttggccgt tagtaatgca gcgcagttgc tccacgacca gaccaacgtg atgatcgtaa
     3721 agatggatac ggtttttcat cgcctgctgg acagcatgcc agtcggcctg ttcgaaacgc
     3781 tgctgcgccc cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa gccttgcaaa
     3841 atggtttgag caatcagtaa ttccaggcca cgcggcattt agcgccctca tcaggagcag
     3901 agaattgccg gatgcggcgt aaacgcctta tccagcctac gttcggcaac ggttgtaggc
     3961 atgataagac gcgcaagcgt cgcatcaggc atcgtgcacc gattgccgga tgcggcgtaa
     4021 atgccttatc cggcctacag tcagcaacgt ttgtaggttg cttagaactg cgattcttca
     4081 gtggagccag tcagcgcggt gactgaagac gtgccgccct gaataatagt cgtcacttta
     4141 tcgaagtaac ctgtacccac ttcctgctgg tgagatacga aggtatagcc atctttcgcg
     4201 gcggcaaatt ccggctgctg cactttctca acatagtgct tcataccttc gccctgcgca
     4261 taagcgtttg ccaggtcgaa catgttaaac cacatgctgt ggatacctgc cagggtgatg
     4321 aactggaact tgtagcccat atccgacagc tgctgctgga agctggcaat agttttgtcg
     4381 tcgaggtttt tctgccagtt gaacgacggc gagcagttat aagccagcag tttgcccgga
     4441 tatttcgcgt ggatagcttg tgcaaagcga cgcgccagtt ccagatccgg cgtggaggtt
     4501 tcacaccaga ccaggtcagc atatggcgca tacgccaggc cacggctgat cgcttgctca
     4561 atgcccgcat gagtacggaa gaagccttca ctggtacgct cgccggtaat aaattcgctg
     4621 tcatacgggt cgcaatctga ggtgatcaga tccgccgcat cagcatcggt acgtgcaacc
     4681 agcagggttg gaacgcccgt cacgtcagct gccagacgcg ccgcgaccag tttctgaata
     4741 gcttcctgag ttggcactaa aactttgccg cccatgtgac cgcatttctt cactgacgcc
     4801 agctgatctt cgaagtgaac tgccgcagca ccggcttcaa tcatcgcttt catcagttca
     4861 aaggcattca ggacaccgcc aaaaccggct tccgcatcgg caacgatcgg caggaagtaa
     4921 tcgacatagc gcggatcgcc cggctcaatg cccacggacc attggatctg atcggcacga
     4981 cggaaggtgt tgttgatccg ctccaccaca gctggcaccg agtttgccgg atagagcgac
     5041 tgatccggat acatgctggc cgccaggtta gcgtccgccg ctacctgcca tcccgacaga
     5101 tagactgctt caatacccgc tttcgcctgt tgcagcgcct gaccgccagt cagtgcaccg
     5161 aggctgttga tgtagctttt ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc
     5221 ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg taatttcacc acatcttccg
     5281 cactgtatgg gcgagtaatg ccttcccaac gcggttgagt ccactctttc tgtaattctt
     5341 caatttgttg tgtacgggtt ttcatgtgca gatgctccat agttatgtgg tggtttacgc
     5401 taacaggcgg tagcctggca gggtcaggaa atcaattaac tcatcggaag tggtgatctg
     5461 ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag aaacgttctt cgcccagttc
     5521 gctggcaatg actttcatct cttcgcccag catctggcgg aacaaggctt tggtcaccgg
     5581 tttgccattg ctcaacgttt tttgatgatg gatccactgc cagatcgagg tacgggaaat
     5641 ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc acgcagccgt tgccggagat
     5701 ccacgcttcg atgtactgca cagccacgcg aatgttagcg cgcatacctt cttcggtgcg
     5761 ttcaccatca caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa
     5821 tacttcaagc tgatttttac gggagccgag aatgtcgttg aataccgcca ttgccgtgtc
     5881 cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt
     5941 atccgctttt actttgttga gcacctggtt attacgctct tcatctttgc tcggaataaa
     6001 cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg caggttttaa tcagcaggcg
     6061 tgagtaagca ttcaggaatg gtttatccat cgtcacggcc tgtctgtctg gcaggacacg
     6121 atcgggatag tttttcaacg ttttgatata gctgaagatg taatcccagc gaccgcagtt
     6181 cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg tccatctgga acacggcggg
     6241 cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc
     6301 tgcatagctg aagacttcgc tccaccaggc cgcttcctgc taggactggg ttttcggcag
     6361 atagaaatag ggaccgctgc cctttgccaa cagtgcctga tagttgtgga agaaatagag
     6421 cgcaaaatca aacaggctac cggggattgc ctcaccacgc caggtgacat gtttttccgg
     6481 caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga gctgataaat
     6541 tttgcctgct tcattggtat agctgatggt accgttaacc gcatcgcgca ggttaatttg
     6601 cccgtcgatc actttgttcc agtctggtgc cagtgaatct tcgaaatcgg ccataaagac
     6661 tttcacattg gcgttcagtg cgttgatcac catcttgcgc tctaccgggc cagttatctc
     6721 tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat
     6781 ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta tcaatatctt gctgctgctg
     6841 aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag
     6901 aaattctacc gcttcggcag taagaatttg cttctcctgc tcgccatacg gccttgtgaa
     6961 agccagttca tcggttgttg ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc
     7021 gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt ttcgttcagc acaactaaga
     7081 ctactcattt aaaatgcaaa atcaaaaaca atttccattt taattaaaaa tacttgataa
     7141 cttattgata acaatatgaa taaaatttat tcacttgctg aggtgcgttt cggaaataaa
     7201 aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc ttaacgatcg actatcacag
     7261 aagattaatc cagcgttgga ttcatgtgcc gtagatcgta tggcgtgatc tgatagacgt
     7321 aatagttgag ccagttggta aacagcaaat taccgtgact acgccagctc gctcgcggtt
     7381 tgttttgcgg atcgttgtgc gggaaatagt tatacggtac atccgggtct agtccggctt
     7441 ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc atattcggga tggcccgtca
     7501 caaaggcaat gcgcttatct ttgctggcaa acagatatgc atccccttct tccgtctctg
     7561 ccagaatttc cagatcggtg taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg
     7621 aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa
     7681 tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt ttgcttagga atgccgtaga
     7741 ggatattgag cgcggcctgt accgcccagc agacaaacag cgtcgaggtg acgtgatctt
     7801 tcgaccactc cagcacctgt ttgatctgcg gccagtaagc gacatcatta aactccacca
     7861 ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt ctgttcctga atatcttcaa
     7921 agttacagta gaagttgttc agatgctctg cgggcgtgtt gcgcgattca cgggaatcga
     7981 tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga aagcaggcgc agaaactgat
     8041 tttcagtttc aatcttcttc ggcatcaggt taaggatcag aaccttaagt ggacgaattt
     8101 cctgaccaga cgcacgagaa gttgtcatca caaagacgtt ttcttcacgc aagaaattga
     8161 cggcgggtag ctcgtccggc acacgaatcg gcataacctg attacctcac tacatacgct
     8221 tatacgttta gacatccaga tagctgaaga taaccagaaa atttgccaat gtcgagcctg
     8281 catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt agaatataag aatgacgaaa
     8341 aggagagaaa atggttatta gtattcgccg ctcacggcat gaggaagggg aggaactcgt
     8401 tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt ttatcagcag agtatcgggc
     8461 cgagctggaa gagctggtgc gttcctttct gccggaagca ccgttgtggg tcgcggttaa
     8521 tgaacgggag cagccggttg ggtttatgtt gttaagtggg cagcatatgg atgcgctgtt
     8581 tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg ctggtggagc atgcgctctc
     8641 gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat gagcaggcgg ttgggttcta
     8701 taagaaggtg ggttttaagg ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta
     8761 tccgttgctg aatctggcgt atgtggggga ataagccgcc cctgccgtgc ctgcccaagc
     8821 tggagaagac gcgccagcgt cgcacccggc atttttttca c
//
LOCUS       DAJTNV010000134         8784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNV010000134 DAJTNV010000000
VERSION     DAJTNV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..554
                     /locus_tag="OO199_003574"
     CDS             <1..554
                     /locus_tag="OO199_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537467.1"
                     /translation="FSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVG
                     MWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALF
                     CVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLAL
                     VIAACVTFTGALCYFFIVKDRIE"
     gene            806..1723
                     /gene="oxyR"
                     /locus_tag="OO199_003575"
     CDS             806..1723
                     /gene="oxyR"
                     /locus_tag="OO199_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5537468.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1706..3106)
                     /gene="sthA"
                     /locus_tag="OO199_003576"
     CDS             complement(1706..3106)
                     /gene="sthA"
                     /locus_tag="OO199_003576"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5537469.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3427..4077
                     /gene="fabR"
                     /locus_tag="OO199_003577"
     CDS             3427..4077
                     /gene="fabR"
                     /locus_tag="OO199_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5537470.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4077..4436
                     /locus_tag="OO199_003578"
     CDS             4077..4436
                     /locus_tag="OO199_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5537471.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4476..5564)
                     /gene="trmA"
                     /locus_tag="OO199_003579"
     CDS             complement(4476..5564)
                     /gene="trmA"
                     /locus_tag="OO199_003579"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5537472.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5678..5868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5933..7777
                     /gene="btuB"
                     /locus_tag="OO199_003580"
     CDS             5933..7777
                     /gene="btuB"
                     /locus_tag="OO199_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5537473.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7722..8579
                     /gene="murI"
                     /locus_tag="OO199_003581"
     CDS             7722..8579
                     /gene="murI"
                     /locus_tag="OO199_003581"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5537474.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2021 a         2131 c         2353 g         2279 t
ORIGIN      
        1 gtttctctgg cgtcaattat accggttggc tttacattgc gtggttaccc ggttatttgc
       61 aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt cctttcctcg
      121 cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg aaaaaaggct
      181 acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg tcggcattag
      241 gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt atctcaatgg
      301 cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt
      361 cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt gtttttgctt
      421 cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt aatctggcgc
      481 tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt attgtcaaag
      541 atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag aataaaaaaa
      601 agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact
      661 ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc tgtgagcaat
      721 tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta tcgccatgaa
      781 ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc tggtggcatt
      841 ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc agccgacgct
      901 tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg agcggaccag
      961 ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct
     1021 gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga tgtccggacc
     1081 gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata ttatcccgat
     1141 gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga cccaccagtt
     1201 actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag
     1261 cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta tctatgaaga
     1321 tcacccgtgg gagaaccgcg aatgcgtact gatggccgat ctggcagggg aaaaactgct
     1381 gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct ttgaagccgg
     1441 ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca acatggtggc
     1501 ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc gcaaacgcga
     1561 tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg gcctggttta
     1621 tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca tccgcgcaag
     1681 aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt aacgcagcta
     1741 cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag tactcaatag
     1801 tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt tcggcagcgc
     1861 gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac aaaattttca
     1921 gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac tgggcgcggc
     1981 ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc acagagctga
     2041 tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg gtggcttcgc
     2101 cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat gccaggctcg
     2161 gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt
     2221 tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac gccagcgaat
     2281 cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt tttttacccg
     2341 acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac tcttcgttgt
     2401 gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac atctcttgat
     2461 cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca tccataccgc
     2521 ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt acatggcgcg
     2581 gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa
     2641 catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca gcggttagtg
     2701 tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt
     2761 tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt
     2821 gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg agcagtcggg
     2881 aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg gcgtgacgga
     2941 gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt tgataacgct
     3001 cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg
     3061 ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta gggcttacct
     3121 gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc caattgatcc
     3181 ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct tacttttgcc
     3241 ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag gtttccgctg
     3301 tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt cacaatactg
     3361 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
     3421 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
     3481 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
     3541 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
     3601 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgccaggcg
     3661 cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac atttatggag
     3721 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
     3781 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
     3841 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
     3901 gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga acaacgtcgg
     3961 caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc ttattactgg
     4021 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
     4081 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt ttatcgatta
     4141 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
     4201 ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc atgtcggtag
     4261 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
     4321 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
     4381 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
     4441 tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag taatacgccg
     4501 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
     4561 gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga gatatacaga
     4621 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggtggg
     4681 tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag gcggttaaac
     4741 tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
     4801 ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg tttggcgatt
     4861 tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt accgttgccg
     4921 cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
     4981 atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg acgatagatc
     5041 atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag cgcgattttg
     5101 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag tgcttccgct
     5161 tcctgacgcc actcatcatc cagcttttta tggtacagca gggaaaccac cgcctgatta
     5221 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
     5281 acacccgcaa tcatcgccgt catcaactgg ttgatgagtt cactggcggc ggggaagcta
     5341 tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
     5401 tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga gcgaaacact
     5461 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
     5521 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
     5581 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
     5641 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc
     5701 tgtgagttaa tagggaatcc agtgcgaatc tagagctgac gcgcagcggt aaggaaaggt
     5761 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
     5821 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
     5881 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
     5941 aaaagcttcg ctgctgacgg cgtgttccgt cacggcattt tccgcttggg cacaggatac
     6001 cagcccggat actctcgtcg ttactgctat ccgttttgaa cagccgcgca gcactgtgct
     6061 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
     6121 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
     6181 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ctggtgttaa ttgatggcgt
     6241 ccgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
     6301 tgtccagcgt attgaatata tccgtggacc gcgctccgct gtttatggtt ccgatgcaat
     6361 aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
     6421 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
     6481 aacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
     6541 tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
     6601 tggggcgctg gagcataact ttactgatgc ctggagcggc tttgttcgcg gctatggcta
     6661 tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc tcgatacccg
     6721 taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac tgattaaatc
     6781 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
     6841 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
     6901 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
     6961 gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca tctatctgac
     7021 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagcg acgataactc
     7081 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
     7141 tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc aactgtatgg
     7201 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
     7261 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
     7321 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
     7381 cgtcgaggcg acagccaatt ttgataccgg accactgacg catactgtga gttatgatta
     7441 tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
     7501 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
     7561 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
     7621 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcacctga cagttcgtgg
     7681 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
     7741 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
     7801 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
     7861 tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt
     7921 ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat ccccttgcgc
     7981 tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta cgcgaaaagt
     8041 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
     8101 atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat actcatgagc
     8161 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
     8221 agttggctga agcgaagctg catggcgaag atgtttctct ggatgcacta aaacgtatcc
     8281 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
     8341 tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg ctggtggatt
     8401 ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc ccggatgcaa
     8461 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
     8521 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
     8581 ttggttgaat gttgcgcggt cggaaaatta ttttaaattt cctcttgtca ggccggaata
     8641 actccctata atgcgccacc actgacacgg aacaacggca aacacgccga cgggtcagcg
     8701 gggttctcct gagaattccg acagagaaag caaaaataaa tgcttgactc tgtagcggga
     8761 aagcgtatta tgcacacccc gcgc
//
LOCUS       DAJTNV010000135         8730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNV010000135 DAJTNV010000000
VERSION     DAJTNV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(857..2053)
                     /gene="hemY"
                     /locus_tag="OO199_003582"
     CDS             complement(857..2053)
                     /gene="hemY"
                     /locus_tag="OO199_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5537475.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2056..3267)
                     /gene="hemX"
                     /locus_tag="OO199_003583"
     CDS             complement(2056..3267)
                     /gene="hemX"
                     /locus_tag="OO199_003583"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5537476.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3289..4029)
                     /gene="hemD"
                     /locus_tag="OO199_003584"
     CDS             complement(3289..4029)
                     /gene="hemD"
                     /locus_tag="OO199_003584"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5537477.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4026..4967)
                     /gene="hemC"
                     /locus_tag="OO199_003585"
     CDS             complement(4026..4967)
                     /gene="hemC"
                     /locus_tag="OO199_003585"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5537478.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5354..7900
                     /gene="cyaA"
                     /locus_tag="OO199_003586"
     CDS             5354..7900
                     /gene="cyaA"
                     /locus_tag="OO199_003586"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5537479.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7940..8260)
                     /gene="cyaY"
                     /locus_tag="OO199_003587"
     CDS             complement(7940..8260)
                     /gene="cyaY"
                     /locus_tag="OO199_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5537480.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8308..>8730)
                     /locus_tag="OO199_003588"
     CDS             complement(8308..>8730)
                     /locus_tag="OO199_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5537481.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1993 a         2250 c         2242 g         2245 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttatttaagt gatactggtt
       61 gtctggagat tcagggggcc agtctagcag ttgacggggt atctgctgcg ctggccatga
      121 gaggaagagc ggcggcgaca gcaacaacaa gacgtttggg tgaaaacgaa aattccatgc
      181 aaaatgctcc ggtttcatgt catcaaaatg atgacgtaat taagcattga taattgagat
      241 ccctctccct gacaggatga ttacataaat aatagtgaca aaaataaatt atttatttat
      301 ccagaaaatg aattggaaaa tcaggagagc gttttcaatc ctacctctgg cgcagttgat
      361 atgtaaggca ggtttattat atcgcgttga ttattgatgc tgtttttagt tttaacggca
      421 attaatatat gtgttattaa ttgaatgaat tttatcattc ataataagta tgtgtaggat
      481 caagctcagg ttaaatattc actcaggaag ttattactca ggaagcaaag aggattacag
      541 aattatctca taacaagtgt taagggatgt tatttcccga ttctctgtgg cataataaac
      601 gagtagatgc tcattccatc tcttatgttc gccttagtgc ctcataaact ccggaatgac
      661 gcagagccgt ttacggtgct tatcgtccac tgacagatgt cgcttatgcc tcatcagaca
      721 ccatggacac aacgttgagt gaagcaccca cttgttgtca tacagacctg ttttaacgcc
      781 tgctccgtaa taagagcagg cgttttttta tgcatcagaa aggtcccgga ggtgctttcc
      841 tccgggtgag aaggaactac tgtggcgggt tattctgcaa cgttaacatc aaaccgtcgc
      901 gacgcatcgc tgcagcttct tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg
      961 cgtaatcgta ggcgtccgga cgttgtttca gcgccgcgcg gaaggcgagc gatgcttcct
     1021 gccattcacc gtgcttcatc agtgactgac ccaatgtgct ccacaacagc gggcgatcac
     1081 cgacgttttt gatttgctgg cgtagcactt tttccagctg ttccgggtta ttggttttca
     1141 gacgcgggat cggcagcagt aggcgatcgt catactggcg tttcaggccg tcgatgataa
     1201 tttgctgggc agtatcatga tcgtcacatt caataagatg ttccgccatt gccacctgca
     1261 acgctacctg atgacgcgtt ttccggcttt ggtttttcca ccagttacgc aaaccttcac
     1321 taccgttatc ggcacgcgcc tgatccatca ggccaatcca tgcctgttgt tccagcattg
     1381 cacgatgttc ttcatcacca acatgggctt tcgccattga tgggatgata tccagcagag
     1441 aactccatgc ccctgtgcgg atatacgcct gttccgccag acgtaatact tcaggatggc
     1501 gtggcgtaac ttccagcagc ttatccacgc cgtggcgtgc agcatggttt tcattacggg
     1561 ccagttgcag acgtacacgg gtgatttcta ccggaatggt gtcattgccg gccagctccg
     1621 ctgcgcgttc cagatgttgg ttggcgcgtg cttcatcacc acgttgttgc gcggcttcgg
     1681 cagccagtag atagttcacc accggttgtt cagcgtgatc ggcatttttc gccatcagct
     1741 tttcaacttg ctgataatca ccttccgcca gtttcagcag cgcctgttcg gtctgcttac
     1801 gcgcacggcg acgcttacgt ccgacaaacc atccacgggt gtgcgcgcca gtgcggaaga
     1861 tccgccgcag tagccactca atggcaaaca gcactaccat cgccagaatc aatatgatcg
     1921 ccaggcccgt gacgctggtt tcgatattgt agttgtcggt ctggatcagc acataaccct
     1981 gatgaccggc aatcatcggg ccaaccacta tccccgcaat cagcaaaaca aagagtaata
     2041 acacttttag catggttatt ctccttgcgg cgcggctgcc ggagtatcac cttgcggtgc
     2101 aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccccgc
     2161 tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca gcatcgcctg
     2221 gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc
     2281 gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga
     2341 gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag cttgtgctgc
     2401 gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg gcgctaacag
     2461 cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa agttctgcca
     2521 gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt caccgtctga
     2581 atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta cctgatttga
     2641 aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg cagaaaggct
     2701 ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat tcatatccgc
     2761 caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct gatcgctcca
     2821 cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc aggttttagc
     2881 atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt ttgccagcgt
     2941 ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa tgccttccag
     3001 ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag ccagggcatc
     3061 gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac cgacacccgc
     3121 cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt tcttactctt
     3181 tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac
     3241 ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc
     3301 ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc agcccagttc
     3361 ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta gtaaccagtg
     3421 ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac
     3481 aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg
     3541 gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca ccgtcagggt
     3601 atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat
     3661 attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gtgggtagag
     3721 aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa tggcgaaata
     3781 agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac
     3841 cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca gttgatcagc
     3901 aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg
     3961 ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc gggtgaccag
     4021 gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg cgcgccgtta
     4081 ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg
     4141 cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca tagccagatt
     4201 tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg
     4261 gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggca
     4321 agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc accttgtcct
     4381 accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc cagacctaaa
     4441 cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc tagtttgctc
     4501 aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc
     4561 agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg taacgcatcc
     4621 agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc gcaaatagtg
     4681 accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg tacggcgata
     4741 tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc ttttccgcct
     4801 actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca
     4861 acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt
     4921 gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc
     4981 attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt tgtggtgaaa
     5041 cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac aggcgtttca
     5101 cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc
     5161 tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc
     5221 gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat
     5281 cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat
     5341 caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa
     5401 tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag
     5461 tctactgccg acattgttgc actatcacca tccgctaatg ccgggttacc ttgatggtaa
     5521 cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga
     5581 gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc ttccaattac
     5641 tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga
     5701 tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac tacaacgtaa
     5761 atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat
     5821 tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc
     5881 cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc tcgccggtaa
     5941 gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat
     6001 gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg gtggcttaag
     6061 ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga
     6121 ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc
     6181 gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg agattgtatc
     6241 gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc tgacggcgat
     6301 tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag tgtgcgaaaa
     6361 actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag
     6421 cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta actggaagat
     6481 tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga gctaccgtaa
     6541 tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc aggatatcgg
     6601 cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag tgacgctggt
     6661 aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt atgtgccgcc
     6721 gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcgat
     6781 catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg cgtgggcatg
     6841 gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg gcattgtcga
     6901 tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc tgcgcttacc
     6961 tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg cgattatcgt
     7021 taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt ttgatttccg
     7081 taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct
     7141 gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat
     7201 gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac cgccagatag
     7261 cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc gcgtgcagca
     7321 actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa ccgggcgttt
     7381 caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc tgaatgtatc
     7441 ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca
     7501 cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt
     7561 tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga atggctttaa
     7621 tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa
     7681 agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc gcttcaccta
     7741 cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg
     7801 tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc ccgccaatca
     7861 ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc ggaaagcgag
     7921 gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt tgcgtcgccg
     7981 cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat tcatcgcctt
     8041 tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc agcggctcct
     8101 ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg ccgccgttga
     8161 tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca atggtcagcc
     8221 acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag
     8281 tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct ctgctgctgc
     8341 gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa cttcacgtac
     8401 gttctcctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc ttacctctgt
     8461 atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt tagttagcga
     8521 accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa agtaagcatg
     8581 ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca gcgggattat
     8641 ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat ctttatattg
     8701 cgcaatattg ccagtaatca gatggaattg
//
LOCUS       DAJTNV010000136         8394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNV010000136 DAJTNV010000000
VERSION     DAJTNV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            2..815
                     /gene="ttdA"
                     /locus_tag="OO199_003589"
                     /pseudo
     CDS             2..815
                     /gene="ttdA"
                     /locus_tag="OO199_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            812..1417
                     /gene="ttdB"
                     /locus_tag="OO199_003590"
     CDS             812..1417
                     /gene="ttdB"
                     /locus_tag="OO199_003590"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5537482.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1465..2928
                     /gene="ttdT"
                     /locus_tag="OO199_003591"
     CDS             1465..2928
                     /gene="ttdT"
                     /locus_tag="OO199_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5537483.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2971..3984)
                     /gene="tsaD"
                     /locus_tag="OO199_003592"
     CDS             complement(2971..3984)
                     /gene="tsaD"
                     /locus_tag="OO199_003592"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5537484.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4222..4437
                     /gene="rpsU"
                     /locus_tag="OO199_003593"
     CDS             4222..4437
                     /gene="rpsU"
                     /locus_tag="OO199_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5537485.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4548..6293
                     /gene="dnaG"
                     /locus_tag="OO199_003594"
     CDS             4548..6293
                     /gene="dnaG"
                     /locus_tag="OO199_003594"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5537486.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6488..8329
                     /gene="rpoD"
                     /locus_tag="OO199_003595"
     CDS             6488..8329
                     /gene="rpoD"
                     /locus_tag="OO199_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5537487.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2253 c         2252 g         1862 t
ORIGIN      
        1 agtgaataag ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc
       61 tgatgacgtg gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa
      121 aattatctac catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc
      181 ctgtcaggac accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct
      241 tggcgagctg caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact
      301 acgtcacaat gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg
      361 cgtaccgtgg gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta
      421 catggcaggc ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg
      481 ctacgaaggc gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg
      541 tccaccggta ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc
      601 gcgtaaagcc attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact
      661 ggagctacgc ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg
      721 caacagttca gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct
      781 ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa
      841 agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt
      901 gacctgccgc gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga
      961 tctcaacggc aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gtgacaaatg
     1021 ggagatggtc tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt
     1081 tattgagcag accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga
     1141 agaaggctgc cagaaattca aggcgttaca tgtgattttc ccagcaggct gcgcggtgct
     1201 ggcggcaacc caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga
     1261 gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca
     1321 cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt
     1381 ggaagagatc tgcgagcacg tccattacat caaataaccc tcccggagag gctcacccct
     1441 ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgcg
     1501 ccgctggcgg tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat
     1561 acctggctct actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg
     1621 ccgggtgccg tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg
     1681 ctgttcagcc ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc
     1741 tgggcagttt ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt
     1801 ggcacaggct atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag
     1861 atggggcatc gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca
     1921 cctgtaacac cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac
     1981 ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac
     2041 atcatgtgga tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg
     2101 gcacctaact tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg
     2161 ggcgactggt tcctcgggat gttgccgctc agcattttac tggttctgct ggttccctgg
     2221 ctggcttacg tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca
     2281 gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg
     2341 atggtaggcg cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc
     2401 ggttacagcg tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc
     2461 agtaataaag cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc
     2521 ggactcaaca ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt
     2581 ggttattcgc cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac
     2641 tttttcgcca gcgccacggc gtatacctgc gccctcgcac cgatgatgat cgccgccgcg
     2701 ctggcgatgc cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt
     2761 ctggggagca ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt
     2821 tatctgccaa cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg
     2881 ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg
     2941 ccggaaattg ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca
     3001 gcgcggacgc acgctaacac cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc
     3061 ggcataggcg atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc
     3121 gccgcggcgt tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac
     3181 gccgcccgcc atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat
     3241 catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc
     3301 gtcggtgccg ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa
     3361 atccagcccc ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg
     3421 agccgccatt ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt
     3481 tttatcaaac gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc
     3541 aatgccagtc acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg
     3601 aaattccggc gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac
     3661 cgggattgcc ggaacgttcc aggcaaacgc cagagaacgc cccacggttg cgccaaccag
     3721 cagtgcgccg actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt
     3781 taaaccagac tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg
     3841 ggaggccagt tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa
     3901 ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga
     3961 agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta
     4021 ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc
     4081 ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat
     4141 tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa
     4201 ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac
     4261 gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt
     4321 cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg
     4381 aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc
     4441 cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag
     4501 gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa
     4561 tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg
     4621 cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg
     4681 agaaaacccc gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg
     4741 gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa
     4801 cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc
     4861 ccagccagat cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata
     4921 cgttttacca acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa
     4981 aacgcggatt aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct
     5041 gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg
     5101 cggggatgtt ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga
     5161 tgttccccat tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca
     5221 acgatacccc caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc
     5281 tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg
     5341 aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt
     5401 taggtacgtc aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca
     5461 tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg
     5521 cgctgcctta catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag
     5581 accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg
     5641 cgatgccact ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc
     5701 ctgacgggcg cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg
     5761 aaacgctgcg aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca
     5821 gccagcttga acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc
     5881 agctaaaacg cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag
     5941 cgacgttggt cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat
     6001 tcagagaact ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag
     6061 agcactatcg tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata
     6121 tagcagataa gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt
     6181 cgctgcttga actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca
     6241 acgaagaacg cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg
     6301 gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa
     6361 atatataagt acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt
     6421 aacggctgaa ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata
     6481 ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga
     6541 gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc
     6601 agatcagatc gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga
     6661 agcaccggat gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga
     6721 agccgccgcg caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt
     6781 acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga
     6841 catcgctaag cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc
     6901 ggaagcgatc acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct
     6961 gtccgatctg atcaccggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc
     7021 cactcacgtc ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga
     7081 agaagatggc gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg
     7141 cgaaaaattt gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa
     7201 aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca
     7261 gttccgcctg gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga
     7321 ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc
     7381 gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc
     7441 ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca
     7501 tcgcgccctg caaaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt
     7561 taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga
     7621 gatggttgaa gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg
     7681 cttgcagttc cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa
     7741 attcgaatac cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc
     7801 gatcacccgt tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga
     7861 gactatcaac aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc
     7921 gacgccggaa gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct
     7981 gaagatcgcc aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca
     8041 tctgggggat ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga
     8101 aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt
     8161 tctgcgtatg cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa
     8221 acagttcgac gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact
     8281 gcgtcacccg agccgttctg aagtgttgcg tagcttcctg gacgattaat cggtaggccg
     8341 gatcaggcgt tacgccgcac ccggcactag tctctctgca caaacgccac cttt
//
LOCUS       DAJTNV010000137         8264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNV010000137 DAJTNV010000000
VERSION     DAJTNV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            69..317
                     /locus_tag="OO199_003596"
     CDS             69..317
                     /locus_tag="OO199_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5537488.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            382..750
                     /locus_tag="OO199_003597"
     CDS             382..750
                     /locus_tag="OO199_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5537489.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            844..1497
                     /gene="nfsB"
                     /locus_tag="OO199_003598"
     CDS             844..1497
                     /gene="nfsB"
                     /locus_tag="OO199_003598"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5537490.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1605..2852
                     /gene="ybdG"
                     /locus_tag="OO199_003599"
     CDS             1605..2852
                     /gene="ybdG"
                     /locus_tag="OO199_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5537491.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2920..4296)
                     /gene="pheP"
                     /locus_tag="OO199_003600"
     CDS             complement(2920..4296)
                     /gene="pheP"
                     /locus_tag="OO199_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5537492.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4397..7540)
                     /gene="cusA"
                     /locus_tag="OO199_003601"
     CDS             complement(4397..7540)
                     /gene="cusA"
                     /locus_tag="OO199_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5537493.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7552..8070)
                     /gene="cusB"
                     /locus_tag="OO199_003602"
                     /pseudo
     CDS             complement(7552..8070)
                     /gene="cusB"
                     /locus_tag="OO199_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2016 a         2307 c         2013 g         1928 t
ORIGIN      
        1 taagccggga attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta
       61 acaatgttat gaaacaccct ttagaaacct tgaccaccgc agcaggcatt ttgctgatgg
      121 ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac
      181 tggtgaccat gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt
      241 ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt
      301 tctcgctggc ggattaaacg tcgattatcg ccgaacttcg cggcatactt tgcttattct
      361 ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgccta
      421 gctcttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt
      481 aaaattggcg gcaagatttt tatgctgtcg tcggagctac gcggcgtccc ctttatcaat
      541 ctgaagtccg atccacaaaa atccctgtta aatcagcaaa tatacccaag cattaagcca
      601 gggtatcaca tgaataaaaa gcactggatt tcggtgtatt ccggcgagga aatctccgaa
      661 gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa
      721 cgcgatcaaa aaagagtgcg tccaggctaa agcgggaatc tatagcgcat ttttctcgct
      781 taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattacttac acatggagtc
      841 tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagc
      901 aaaaaactta ccccggaaca ggccgagcag atcaaaacgc tactgcaata cagcccatcc
      961 agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt
     1021 gttgccaaat ccgctgccgg taattatgtg ttcaacaaac gtaaaatact tgatgcctcg
     1081 cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt
     1141 gaccaggaag atgccgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa
     1201 ggtcgcaagt tcttcgccga tatgcaccgt aaagatctgc atgatgatgc agagtggatg
     1261 gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc gctgggtctg
     1321 gacgcggtgc ccatcgaagg ttttgacgcc gcaatcctcg atgcagaatt tggtctgaaa
     1381 gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt
     1441 aacgctacgc tgccgaaatc tcgtctgccg caaaacacca ccttaaccga agtgtaattc
     1501 tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct
     1561 gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat
     1621 cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt
     1681 tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc
     1741 ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc attacccaga
     1801 ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg
     1861 tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc gcgcagttgt
     1921 ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt ttgaatctgg
     1981 cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac
     2041 tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag
     2101 cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc
     2161 cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg aaactgggcg
     2221 actggctgga aatgccgaaa tacggcgcgg atggcgcggt gatcgatatt gggttaacca
     2281 ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt
     2341 ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaagcgca
     2401 gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga
     2461 ataaagcgca tttgttaaag ccttatttaa ccagccgcca tcaggaaatt aatgagtgga
     2521 atcgccagca aggttctacg gagtcggtat taaacctgcg ccgaatgacc aatattggaa
     2581 cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga
     2641 ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag atctacgcgt
     2701 ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca
     2761 tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc ggcaatgata
     2821 ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat
     2881 gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt
     2941 aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca agcgcatatc
     3001 atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa gatagttgcc
     3061 gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca tcgccgcgcg
     3121 aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcaacgttg ctaccaccag
     3181 cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga
     3241 agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc tgacgcgagt
     3301 caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta
     3361 aaccccgctg ttatacaccg acagcgatgc cactagaatg acgaagttca gcgcagaagc
     3421 taccacattg ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac
     3481 ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa acagcaggat
     3541 gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc
     3601 ggctgtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa
     3661 aatcagccca ttccagccgg tggcgaagaa accaccgtag cgccagaggt tgtcgatact
     3721 ggctttctcg ccgccgtaac cagaaaacag cagccacagg ccaaagccga tcataccgat
     3781 gatcgccagc actttaatca gcgcaaacca gaactcggtt tcgccatata agcgcacgtt
     3841 caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac
     3901 atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag
     3961 cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt
     4021 ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct cgccaagctg
     4081 gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc
     4141 cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg cgccacccag
     4201 cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga
     4261 cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt
     4321 gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta
     4381 aacgaggtat cctgctttaa ttccgtaccc gatgtcggtg cagccacatc agcttatacg
     4441 ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggtg
     4501 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
     4561 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat
     4621 acaacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac gacggctcgg
     4681 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg
     4741 caagggcaat aaagccggta cccgtcgcca cagaaagatg aaagcccatc caccacagta
     4801 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
     4861 cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga
     4921 gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
     4981 tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca
     5041 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
     5101 tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct
     5161 gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg cgccagcttt
     5221 gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg
     5281 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg
     5341 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc cgctcagcaa
     5401 gcgcagaagc tacgcctggc accgttcgcg ccacttcttc aatttgctca gccatcgcgt
     5461 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
     5521 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagac
     5581 gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc cgccactgat
     5641 cctgtggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg
     5701 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
     5761 tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
     5821 gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
     5881 gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt ttcggccagt
     5941 gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcggattac
     6001 tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg atcactacga
     6061 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac gggccaaaca
     6121 gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg ataatcagca
     6181 gactgataaa cagcgccggc cccacttcaa cagacgcatc ggtgatcacc tgccagcgcg
     6241 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg
     6301 cattctcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccac
     6361 ccagcgacat aatattggca ttcagcccct ggaagggcat gacaataaaa gcaatacaca
     6421 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
     6481 cgcagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
     6541 cccggtcaat gagctggctg cggtcgtatg ttgtaactat ctccacgcct tccggcagac
     6601 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
     6661 cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc
     6721 cccggcgcat cttcgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc
     6781 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc
     6841 tggcgcgcac catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt
     6901 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
     6961 gaggatcgat aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa
     7021 cgtcaggaat ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg
     7081 caagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
     7141 tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
     7201 ggtactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat
     7261 acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca
     7321 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgcggt gcctggccgg
     7381 gatagctggt tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg
     7441 tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa
     7501 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
     7561 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
     7621 aatcaggaac aggccgccgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
     7681 ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc
     7741 atcaacggta atcacccgct gttcgttgcc ggtatcaatc agcgcctgtg acggaatgag
     7801 cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg gttttagcgc
     7861 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
     7921 aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag
     7981 ggtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg cagcagtgac
     8041 ccacaccggg tccataccct gaattcgcga ttacgcgcaa tccccctgag aaccgccatg
     8101 aacaagggtt tcatgatttg tgattttagt ttggtacgca atttggtaca caacactatt
     8161 ttcacttcag cgggtagtca tcaaactcac cataacgagc atcgttgatg atgtatgtga
     8221 tcaccacaaa tatcacatct ggactagttc cgtcttcatg cgca
//
LOCUS       DAJTNV010000138         8059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNV010000138 DAJTNV010000000
VERSION     DAJTNV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO199_003603"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO199_003603"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5537494.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO199_003604"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO199_003604"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5537495.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO199_003605"
     CDS             3115..3399
                     /locus_tag="OO199_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5537496.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO199_003606"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO199_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5537497.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO199_003607"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO199_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5537498.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..>8059)
                     /gene="fdnG"
                     /locus_tag="OO199_003608"
     CDS             complement(5170..>8059)
                     /gene="fdnG"
                     /locus_tag="OO199_003608"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5537499.1"
                     /translation="SVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAG
                     LLDYVNSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTV
                     NRWLSTGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAM
                     TNHWVDIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADI
                     YAPIRSGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAE
                     KRQYDKSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADF
                     LKVCEVLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNAL
                     RGHSNIQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPK
                     FFVSLMKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVA
                     SFPDKNKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCF
                     AEEDGSIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMK
                     MSWNYKQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASS
                     CWIYTGSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDP
                     KRMLIQWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYE
                     PIETPLGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHAL
                     LNAIAQPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQV
                     ETVGIPIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         1920 a         2166 c         2065 g         1908 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaa
//
LOCUS       DAJTNV010000139         7783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNV010000139 DAJTNV010000000
VERSION     DAJTNV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            53..586
                     /gene="ymdB"
                     /locus_tag="OO199_003609"
     CDS             53..586
                     /gene="ymdB"
                     /locus_tag="OO199_003609"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5537500.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            528..2009
                     /gene="clsC"
                     /locus_tag="OO199_003610"
     CDS             528..2009
                     /gene="clsC"
                     /locus_tag="OO199_003610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5537501.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2017..3174)
                     /gene="mdoC"
                     /locus_tag="OO199_003611"
     CDS             complement(2017..3174)
                     /gene="mdoC"
                     /locus_tag="OO199_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5537502.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3568..5103
                     /gene="mdoG"
                     /locus_tag="OO199_003612"
     CDS             3568..5103
                     /gene="mdoG"
                     /locus_tag="OO199_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5537503.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5096..7639
                     /gene="mdoH"
                     /locus_tag="OO199_003613"
     CDS             5096..7639
                     /gene="mdoH"
                     /locus_tag="OO199_003613"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5537504.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1927 a         1864 c         2059 g         1933 t
ORIGIN      
        1 aaccaaaaag cctgctgtac acttaagaaa caagaaggtg taaggaggcg ttatgaaaac
       61 gcgtattcat gttgtgcagg gtgatattac caaactggcc gttgatgtga ttgtgaatgc
      121 ggctaatccg tcattaatgg gaggcggcgg cgtcgatggg gccattcatc gcgcagcggg
      181 tccggccctg ctggatgcct gtttaaaagt caggcaacag cagggcgatt gccctacggg
      241 gcatgccgtt attacgcttg caggcgatct tcccgctaaa gccgtagtac acaccgtcgg
      301 gccagtctgg cgtggtgggg aacaaaacga agaccagctt ttgcaggatg cctatctgaa
      361 tagcctacga ctggtggcgg caaacagcta tacgtcagtg gcttttcctg caatcagtac
      421 tggggtttat agttaccctc gtgcggcagc ggctgaaatc gcagttaaaa ccgtttcaga
      481 atttattacc cgtcacgctt tacccgaaca ggtatacttt gtctgttatg atgaagaaaa
      541 cgcccacctc tacgaaagac tccttaccca acaaggagat gaatgatttg ccccggctgg
      601 cgagcgcggt gctgccactg tgttcgcaac atcccggtca gtgtggtctt ttccctctgg
      661 agaaaagtct ggatgcgttt gccgcccggt atcgtctggc cgaaatggca gagcatacgc
      721 tggatgttca gtattacatc tggcaggacg atatgtcggg gcggttactg ttttccgccc
      781 tgttagccgc agcaaagcgt ggcgttcgcg tccgtttgtt gctggacgac aacaatacgc
      841 ccggacttga cgacattttg cgcttgcttg acagtcatcc acgcattgaa gtccggcttt
      901 ttaatccttt ctcgtttcgc ttgctacgtc cgcttggata tatcaccgac ttttcccgtc
      961 tcaatcgccg gatgcacaat aaaagtttca ctgtcgatgg cgtggttacc ctagtgggag
     1021 gacgaaatat tggtgatgcc tattttggtg caggggaaga gccacttttt tcggatttag
     1081 atgtcatggc aataggcccc gtggtagagg atgttgccga tgatttcgcc cgctactggt
     1141 attgcaaatc ggtttcaccc ttacagcagg tgctggatgt cccggaaggt gaaatggcgg
     1201 atcgcatcga gttacccgcc tcctggcata acgatgccat gacgcatcgt tatttacgca
     1261 aaatggaatc cagtccattt ataaatcatc tggttgatgg aacgttgccg cttatctggg
     1321 cgaagacacg tttattaagt gatgatccgg cgaaagggga gggcaaggca aaacggcatt
     1381 cactgttacc gcagcgcctg ttcgatatca tgggctcacc cagtgaacgc atcgatatta
     1441 tctcttccta ttttgtaccg acacgcgcag gtgtggcgca actcttacgg atggtgagaa
     1501 aaggggtaaa gattgcgatc ctaaccaatt ctcttgccgc taacgatgtt gctgtcgtcc
     1561 atgccggata cgcgcgctgg cgcaaaaaat tgctccgcta tggcgtggaa ttatatgaac
     1621 tcaagccgac gcgtgaacaa agcagtacgt tacacgatcg cggcataacc ggtaattccg
     1681 gtgccagcct gcatgctaaa acttttagca tcgatggtaa aacggtgttt atcggctctt
     1741 tcaatttcga tccgcgttca acattgctca atactgaaat gggcttcgtg atagagagcg
     1801 aaacgctggc acagttaatt gataaacgct ttattcagag ccagtacgat gcggcctggc
     1861 agctccgtct ggacaggtgg ggacggatca actgggttga tcgtcatgca aagaaagagg
     1921 ttattctcaa aaaagaaccc gccaccagtt tctggaagcg ggttatggtc agactggcgt
     1981 cgatattgcc cgtggaatgg ttattgtaaa tgtggcttaa cgggctggtg ctttatcgtt
     2041 ctcacgctta acaaccggtt taccagagaa caaaaacttc agtaacggga tgcgtaaatg
     2101 aatttcatac agaattatcg caatccctac tacgaatatc aggccacaga gaaaaccaag
     2161 ccagttggag gtgatgtgcg gtgtaatgta tgcgccgaaa aacagcgtta acgggtggtg
     2221 aaccagatag ataaacagcg atgcgttaac aaaataagtc acccgcgctg actggaagtt
     2281 aagcaaacgg tggccaaagg agaagaccac attcaccatc cacaggccga ggaccatggt
     2341 gatcaccgac tcggtttcgt acatccaggc atcgccactg ccatagcgct ggttgagtaa
     2401 ataagcgaca aacgccaatg ctgcagcgag ggtacagcca cgagacggcg tggtaaacaa
     2461 ggctttaaga tgagggaaaa tgaaagccag tgcgccgagg ataaagaacg gtaaataaaa
     2521 tagcgtttgc atgacaataa aattgaacat gccattactc agaatgggtg gatacacaat
     2581 aaaaatcgtt cttcttatta ccgcatagcc gatgccgagg cataaaaaaa tcaccgatag
     2641 ttttaccatc gagaattttt tattcgtttt atcagaattt tctaaattat ttctgatgcg
     2701 cttaaatatc catacacaca gcgtcgtcat gactactaac accagtaaaa accacaggtg
     2761 tgatattaat tcccaggcca acgtattata tttgtcatac aatgatagcc caggccaact
     2821 ttccgctttc cctttgacat attgcagcat aataaattgc ggtaatgtca gtagggggat
     2881 ggctgttaac atcgggatac ctacacgttc gacacgtact ttccaccatt ttttcaaggg
     2941 atagcgtaaa aaaagcatgt aggaaaagta tccggatata acgaaaaata cctgcatgcg
     3001 gaacgagtgg atgaagtcat taaaaagggt tagccacaat gacggttcgg cgctattcac
     3061 atgccatgta tggctcgaat agattaaaga aatatgaaaa gggatcccta acaacatcag
     3121 ccaggcgcgg atggagtcga ggaaatattc acgttgcgcg ggtactggat tcatatatgg
     3181 ttaactaatc tcggattttt cgtcttatcc ctgtcgggtt atgcctttag gcttgttgcc
     3241 atagcgacac cgacctgacc gcgccaggcg caggcttcaa ggtttttatg catagcatca
     3301 tcgctaccac taaccagaat ggaagcgtct gtaagacggt tgataaataa atttgctggc
     3361 aaaccctaca cgaagtcgat gcttctgtct ttaggagaag cacggaaagt gaaaacggtt
     3421 gcaatcaggt gcttaatcca tgagccagcg tgctgaacga taccgggatt cagttgtcgg
     3481 aatggctggt tatccattaa aatagatcgg atcgatataa gcacacaaag ggggaagtgc
     3541 ttactaatta tgaaacataa actacaaatg atgaaaatgc gttggttgag tgctgcagta
     3601 atgttaaccc tgtatacatc ttcaagctgg gctttcagta ttgatgatgt cgcaaagcaa
     3661 gctcaatcct tagccgggaa aggctatgag gcgcctaaaa gcaacttgcc ctccgttttc
     3721 cgcgacatga aatacgcgga ctatcagcag atccagttta atcatgacaa agcgtactgg
     3781 aacaatctga agaccccatt caaactcgag ttctaccatc agggtatgta cttcgatacc
     3841 ccggtcaaaa taaatgaagt gactgccacc gcagtcaaac gaatcaaata cagcccggat
     3901 tatttcactt tcggcgatgt tcagcatgac aaagacacgg taaaagacct tggttttgcc
     3961 ggttttaaag tgctttaccc gatcaacagc aaagataaaa acgatgaaat cgtcagcatg
     4021 ctcggggcca gctatttccg cgtgattggt gcaggtcagg tttatggcct ttctgcccgc
     4081 ggcctagcaa ttgataccgc tttgccatcg ggtgaagaat ttccacgctt caaagagttc
     4141 tggatcgagc gtccaaaacc gactgataaa cgtttaacca tttatgcatt gcttgactcg
     4201 ccgcgtgcga caggtgctta caaattcgtg gttatgccag ggcgtgacac ggttgtggat
     4261 gtgcagtcga aaatctatct gcgcgataaa gtcggcaaac tgggggttgc accgttaacc
     4321 agtatgttcc tgtttgggcc gaaccaaccg tcgcctgcaa ataactatcg tccggagttg
     4381 cacgactcta acggtctgtc tatccatgct ggtaatggcg aatggatctg gcgtccgttg
     4441 aataacccga aacatttagc ggtcagcagc ttctccatgg aaaacccgcg aggctttggt
     4501 ctgttgcaac gaggtcgtga tttctcccgc tttgaagatc tcgatgatcg ttacgatctc
     4561 cgtccaagcg catgggtgac tccgaaaggg gagtggggca aaggcagcgt tgagctggtg
     4621 gaaattccaa ccaacgatga aaccaacgat aacatcgtcg cttactggac gccggatcag
     4681 ctgccggagc cgggtaaaga gatgaacttt aaatacacca tcaccttcag ccgtgatgaa
     4741 gacaaactgc atgcgccaga taacgcatgg gtgcaacaaa cgcgtcgttc aacgggggat
     4801 gtgaagcagt cgaacctgat tcgccagcct gacggtacta tcgcctttgt ggtcgatttt
     4861 accggcgcag agatgaaaaa actgccagag gataccccgg tcacagcgca aaccagcatt
     4921 ggtgataatg gtgagatagt tgaaagcacg gtgcgctata acccggttac caaaggctgg
     4981 cgtctggtga tgcgtgtgaa agtgaaagat gccaagaaaa tcactgaaat gcgtgctgcg
     5041 ctggtgaatg ccgatcagac gttgagtgaa acctggagct accagttacc tgccaatgaa
     5101 taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag cagcattgcc
     5161 gaagactgat atccgcgccg ttcatcaggc gctggatgcc gaacaccgca cctgggcgcg
     5221 ggaggatgat tccccgcaag gctcggtaaa ggcgcgtctg gaacaagcct ggccagattc
     5281 acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctta aggcgatgcc
     5341 agaagcaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg taggccgttt
     5401 ctgggatcgc ctgcgtggac gcgatgtcac gccgcgctat ctggctcgtt tgaccaaaga
     5461 agagcaggag agcgagcaaa agtggcgtac cgttggtacc atccgccgtt acattctgtt
     5521 gatcctgacg ctcgcgcaaa ctgttgtcgc gacctggtat atgaagacca ttcttcctta
     5581 tcagggttgg gcgctgatta atcctatgga tatggttggt caggatttgt gggtttcctt
     5641 tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctttttg cggtactgtt
     5701 ctgttgggtg tccgccggat tctggacggc gttgatgggc ttcctgcaac tgcttattgg
     5761 tcgcgataaa tacagtatat ctgcgtcaac agttggcgat gaaccattaa acccggagca
     5821 tcgcacggcg ttgatcatgc ctatctgtaa cgaagacgtg aaccgtgttt ttgctggctt
     5881 gcgtgcaacg tgggaatcag taaaagccac cgggaatgcc aaacactttg atgtctacat
     5941 tctgagtgac agttataacc cggatatctg cgtcgcagag caaaaagcct ggatggagct
     6001 tatcgctgaa gtcggtggcg aaggtcagat tttctatcgc cgccgccgtc gccgcgtgaa
     6061 gcgtaaaagc ggtaatatcg atgacttctg ccgtcgctgg ggcagccagt acagctacat
     6121 ggtggtgctg gatgctgact cggtaatgac cggtgattgt ttatgcggcc tggtgcgctt
     6181 gatggaagcc aacccgaacg ccgggatcat tcagtcgtcg ccgaaagcgt ccggtatgga
     6241 tacgctgtat gcgcgctgtc agcagttcgc gacccgcgtg tatgggccac tgtttacagc
     6301 cggtttgcac ttctggcaac ttggcgagtc gcactactgg ggacataacg cgattatccg
     6361 cgtgaaacca tttatcgagc actgcgcact ggctccgctg ccgggcgaag gttcctttgc
     6421 cggttcaatc ctgtcacatg acttcgtaga agcggcgttg atgcgccgtg caggttgggg
     6481 ggtctggatt gcttacgatc tcccgggttc ttatgaagaa ttgccgccta acttgcttga
     6541 tgagctaaaa cgtgaccgcc gctggtgcca cggtaacctg atgaacttcc gtctgttcct
     6601 ggttaagggt atgcacccgg ttcaccgtgc ggtgttcctg acgggcgtga tgtcttatct
     6661 ctccgctccg ctgtggttta tgttcctcgc gctctctact gcattgcagg tagtgcatgc
     6721 gttgaccgaa ccgcaatact tcctgcaacc acggcagttg ttcccggtgt ggccgcagtg
     6781 gcgtcctgag ctggcgattg cactttttgc ttcgaccatg gtgctgttgt tcctgccgaa
     6841 gttattgagc attttgctta tctggtgcaa aggaacgaaa gaatacggcg gcttctggcg
     6901 cgttacatta tcgttgctgc tggaagtgct gttttccgtg ctgctggctc cggtacgcat
     6961 gctgttccat acggtcttcg ttgtcagcgc gttccttggc tgggaagtgg tgtggaattc
     7021 accgcagcgt gatgatgact ccacttcatg gggcgaagcg ttcaaacgcc acggctcaca
     7081 gctgctgttg gggttagtgt gggctgttgg gatggcgtgg ctggatctgc gtttcctgtt
     7141 ctggctggca ccgattgtct tctcgttgat cctgtcaccg tttgtttcgg tgatttccag
     7201 ccgtgccacc gttggtctgc gcaccaaacg ctggaaactg ttcctgatcc cggaagagta
     7261 ttcaccgccg caggtgctgg ttgataccga tcggttcctt gagatgaatc gccaacgctc
     7321 ccttgacgat ggctttatgc acgcagtgtt taacccgtca tttaacgctc tggcaaccgc
     7381 aatggcgaca gcgcgtcacc gcgccagtaa ggtgctggaa atcgcccgtg accgccacgt
     7441 tgaacaggcg cttaacgaga cgccagagaa gctgaatcgc gatcgtcgcc tggtgctgct
     7501 aagcgatccg gtgacgatgg cccgtctgca tttccgcgtc tggaattccc ctgaaagata
     7561 ttcttcatgg gtgagttatt acgaagggat aaagctcaat ccactggcat tgcgtaaacc
     7621 ggatgcggct tcgcaataaa aacgtagttg cctgatgcgc tacgcttatc aggcctacat
     7681 cgttcctgca atttattgat tttgcaagat tttgtaggtc ggataaggcg ttcacgccac
     7741 atccggcaaa aacaacgagc caataaaaat accggcgtta tgc
//
LOCUS       DAJTNV010000140         7555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNV010000140 DAJTNV010000000
VERSION     DAJTNV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OO199_003614"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OO199_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OO199_003615"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OO199_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5537505.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OO199_003616"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OO199_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OO199_003617"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OO199_003617"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5537506.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OO199_003618"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OO199_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5537507.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO199_003619"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO199_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5537508.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OO199_003620"
     CDS             complement(6511..7203)
                     /locus_tag="OO199_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5537509.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1923 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgactatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tctggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg taggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggta ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagagaa agcaaaaata aatgattgac tctgtagcgg gaaagcgtat
     7501 tatgcacacc cccgcgccgc tgagaaaaag cgaagcgcac tgctctttaa caatt
//
LOCUS       DAJTNV010000141         7553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNV010000141 DAJTNV010000000
VERSION     DAJTNV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO199_003621"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO199_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5537510.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO199_003622"
     CDS             1102..2562
                     /locus_tag="OO199_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5537511.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO199_003623"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO199_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5537512.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO199_003624"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO199_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5537513.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO199_003625"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO199_003625"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5537514.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO199_003626"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO199_003626"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5537515.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO199_003627"
     CDS             complement(6727..7245)
                     /locus_tag="OO199_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5537516.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1886 a         1767 c         1908 g         1992 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 gacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt ttt
//
LOCUS       DAJTNV010000142         7355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTNV010000142 DAJTNV010000000
VERSION     DAJTNV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(303..2138)
                     /locus_tag="OO199_003628"
     CDS             complement(303..2138)
                     /locus_tag="OO199_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537517.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            2239..2526
                     /locus_tag="OO199_003629"
     CDS             2239..2526
                     /locus_tag="OO199_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5537518.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            2498..4027
                     /locus_tag="OO199_003630"
     CDS             2498..4027
                     /locus_tag="OO199_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5537519.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(4197..5414)
                     /locus_tag="OO199_003631"
     CDS             complement(4197..5414)
                     /locus_tag="OO199_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5537520.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(5781..6767)
                     /locus_tag="OO199_003632"
     CDS             complement(5781..6767)
                     /locus_tag="OO199_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537521.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(6764..7292)
                     /locus_tag="OO199_003633"
                     /pseudo
     CDS             complement(6764..7292)
                     /locus_tag="OO199_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2273 a         1711 c         1370 g         2001 t
ORIGIN      
        1 gattatgtat taatgtcaaa actggcatat tttacagata caatgaacat gcaacccatc
       61 aatctaatag gggcggtatg tacatctgcc catgaatggg atggcgctaa gaatacaaaa
      121 caattagaca tagatcacaa aaacacacca ttctaattga aaaacaaaac aaacaaataa
      181 atcatactat tatagaattt tctcttgcca gacatagata atacccctgc taattttgct
      241 aatggttagc aaaattagca gggtatataa agattattga cccaaaacac actcagatga
      301 attcacgaca gctcaggagg gttattcctg atgtcatctt cacaaataat ggttaacgca
      361 tcatatatca ttctgcgtct gttcctgttt tcggccttat cgccatacaa catcatcaca
      421 tttttaattt catcgaccgg tgatttcaca tagtgaacta atacaccaag cttctcaaca
      481 cattgcagta aagtatattt cttactgcct gtgaaaagcc ctatcatagt ttctttacgt
      541 tcgcgactgg ataatacttt gttttccata atgacttctg ccagcgtctt accatcataa
      601 acatactctt tatctatcag aatatttatc ttatccagca ccttctctac gtcatcaagc
      661 aaacctgtaa tatatggggg cgcaatatat ggtttcgtac tgatcttttc aagcatacaa
      721 ttatacatct taataacacg catcagggta aaattgttcg catcatactg tatttcaccc
      781 tccttatata caggatcaat aagtggtatt ttttcaataa atgcatcctg ataggctttt
      841 acatggtcaa gccaataaaa caacgagcca tactttatat tttttctatt tactttaacc
      901 tccagtgctg tccgaagcaa cgataatgcc cccctgacat atcctgaagg taattcacca
      961 aacaatcctt ttctttccat gtcatcataa tgctgcagca catcattaat tttatttttc
     1021 cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc tggtcacatg aacagcctca
     1081 ttctgcaact gcccaaaata gcttataatc tttttcagat atgcgtcatc acaaccattc
     1141 ttatcattaa tattaaatac aagatctttc agacgggaaa tatattcctg agatttatca
     1201 tcaagtgaaa cactataacg agacaatgtt ccttctgcac tgttttgaaa cttataaaac
     1261 atttccagta atttttttgc ataaggcgtt gtatcaaagc tggtaaagtc ataaagatat
     1321 tgcgcagcgt aaccatacaa accatgaatc cagagtcctt ctctcaccaa cactctgaga
     1381 taatatgcaa ggctttgaag tatcttcgtc tttcgttgta tatcaagtaa gacacttaat
     1441 gaataaaatg cattatagaa tttatcaata ttcctgagca ctttctccag ttcatcctta
     1501 tccagcttct tctcccggta atccttttta tattcatttt tgatagccat gatatattgt
     1561 tgcatcaggc tcttgctttt cccgttatcc ttccgtatag ttcctgtata tttccccaga
     1621 gcatacagct ttccattttc atgaaaatct tccttgttga atcccagttc agccagatat
     1681 tcccgaagat aacgtttaac agcagcttca tcgctttctg tatcagcgtg atcaagacaa
     1741 ggacaatacg atttctgtaa aaaattataa atatgataat aaaaagcaga gtcattctct
     1801 ggtctgtatg tattatacat atctacaaca agaaaaataa ccccaaaagt gcgcagacaa
     1861 tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg gataagaatt catcatgtaa
     1921 taaaaatgtt catatcgggc atatatgatc cgccgttgct cctgactgaa caccccttca
     1981 gcatcaataa tacgactgat ctcattataa cgttgatccc agaatttttg atgcatttca
     2041 aaattggtac tcttccgctt gtgtgccgga ttctcgcctt ccttaaacaa ccccaaaaca
     2101 gtacaaatat catacaacat atttgtaaat gtgcgcatat gctgtgctct ccggttggtt
     2161 tgcattttat cgcagaggac attctgagct taacgtatca aactgacaca acgacatgca
     2221 atgcatatgg agaacaatat gtcaaatcaa accggacagc tttacacgcc ttcacgcatc
     2281 ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa gatctactat ttacgtgaaa
     2341 ataaatccgg cctcgaaata ccacgaccct acctttccca aaccaataag gcttggtagt
     2401 gcatccgtag gctggcgggc atctgccatc gatgagtggc tgatgttaca tacggcacca
     2461 gcctggtctg aacctgaaaa caaactaaag gaacttaatg atgacgacaa aactatcact
     2521 ccctgaactt gacacaacta aactgcctga caggtgccag gcattacttg atgaaatgca
     2581 tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt atgctgacgg tcatggctgc
     2641 ctcggtccag gatacgcatg aagttgaact ttccggaggg caacgtacca gccttcagat
     2701 atttatgtgt ctttcatcag catcaggcag tggaaaaacc tctgcctgcg caaaattaat
     2761 cgctcctgtt catgaaacag aagaagaact acatcaggca tatatcgacg ataaaaaaaa
     2821 ctatgaccga atgatggaaa tgtggacaac agataaaaaa atcctggagc ggagatataa
     2881 aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca cgagcagcac ttgaaaagtg
     2941 tatagcaaat aaacccgaac ctcccgtaca acaggttctc atcgtgaatg acgcgacacc
     3001 agaagggata gctttgaaac tcagccagtc accctctctt ctgttgctgt ctgatgaagg
     3061 aggtactatt ctggataagc gtttcgaacg caaatcggca ctgtacaaca cattatggag
     3121 tgggcaacca gtcaacgttg aacgggcatc cagacccggg ttccggctga atgatgtccg
     3181 tctcactata cttatactga ctcaaccggt aatatttaat aagtttttta ctctcactgg
     3241 cgatcagatc cggggcaatg gctttctggc ccggttgcta ttttgcgaac ccggagcaat
     3301 taaaataatg acgacagaac cgcatactgc cactcctgtt gtgacgcaac aatgtgcaag
     3361 ctgttttcgt aagaaaagtt ttggcagtca gatcagagac tctctgagag catccagaga
     3421 aagacgtgca aaaagtaaag agcgcatctg catgacatta tcaaatgcag catcccgcgc
     3481 actggagcgc tttcacaaag aaaatatgga tactgtcaga cagaatcctc atatgggtac
     3541 atttgaagac attattgtca gaaaaagaga gcaggttgtc aggatagcag cacttctgga
     3601 actggagaaa gatccagact cgacagtaat aacgctggaa agtactaata gcgccattta
     3661 tctcattgaa ttttatttca agcatcttat atataaactt gaatcccttc gggaaatatc
     3721 cccggctgag aaacttgata aatggcttca gaaaagaatt atcacaacag caggttacat
     3781 attccagaaa agttatattt tacaatatgc cccatatgct ctcaggaaaa aatgtgttct
     3841 ggatgaagca cttgacatac tggcggaaca aaggaaaata aggattgatg ataacctggt
     3901 tgtttatata ggaaacacga ttacaccatc cgaactggca aaaaaactaa atattcctgc
     3961 attcgatgcc ggagttttca tttgtgatca tcaaaacata ctgaagtatc accgaaacag
     4021 actatagata ctatcggaca aaaaataaat tatcacaagt gtattattcg ttataataat
     4081 gaataatcgt tatctgagtg caatgccagg ttttcattac gaactggcat tgcatttatt
     4141 tttcaaaaaa gaaagcagta accatttggc tactctgagt atgccagtcc atgaaactaa
     4201 gcgaattcct tagtgttttt agcaagttga gacatcacat aatccgccca tgcctgcatc
     4261 atcggtacac gctgctccag tagatcagta cgatgatatg ccgcctcaac cttatttttc
     4321 agcgtatgag cgagcgccct ttccgccaga tcccgcgaat acccctgttc actacaccag
     4381 tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc gccccggaat gtcactgacg
     4441 gctttctttt tacgcagaaa acttgtcaac accatatcgg aaaggatctg ctgctttctg
     4501 ggtgaaggga acaccagttc atcatgcagg ccacgtatat tttccagaat gtaaatagcc
     4561 tgccgggata aaggaacacg atgctgtagc ctagctttca ttctttctgc aggtatagtc
     4621 catacccgct tatgaaaatc aatttcagcc cagcgcattc ccctggcttc gcccgagcga
     4681 gttgctgtaa gtatcaccat taataacagt gcgcgggtaa cattataagg ttcatcacta
     4741 tacacactgg tcgccacaaa aagcggtaac tgcctccagg gcattgcggg ttggtgttca
     4801 tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca catcaacagg atttgccaca
     4861 caaaaaccgt gcgcccatcc ccactgcata acaacatgaa tgcgctgttt aacccggctt
     4921 gccgtttctg acaaggttaa ccagactgga cgcagtgttt ctgccacgtc cgcagccgta
     4981 atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt aattctcaag cgtcgataac
     5041 cactgcctta catgctttgg attctcccat ccaggagaca gttctgcatg tacacgcctg
     5101 gctgcatcgg caaatgttgg gataacgact ttctcagatt cagccttttt aatctccaga
     5161 ggatcatcac ctgcagcaag ttgctctcgc attatccggg cagtacgtgc agcttcagca
     5221 atactgacct ctgggtaagt tcccaatcca gcattacgtc ttttttgtgt caccggactt
     5281 acataacgaa aaacccattt cccccgcccc tttactgaag aaggatgaag ggtcagtccg
     5341 gtaattcccc catggggcaa tggtttgtca tcaggtttga tatgtcttgc tttcgtatcc
     5401 gtcaatactg ccatacgcta attcccgtca ctctggtatg ccatccagta tgccattagt
     5461 ccctcgcttc actaagaata cattagataa catcggacaa caaaaagtgt aatacattgt
     5521 ttataaagaa attatataac acccctggat taagtcagat ttatttcagg cggtccccct
     5581 caccgccata tttaaagaag agcgcgtacg aaagtacggg cttttttttc gtatattgta
     5641 cacaccgggt gcccccgccc acgtctgtta gggcgagggg aaattgtgca catcaactgt
     5701 ctggttactc agtcaacaat caaaatgcgt aagatagtac agaacatata tgcgttatct
     5761 ggtcacttcc tgtaacttcc tcacttatta aaatgccccc aacggaaata catgtcacat
     5821 gccatcataa aatcgttatg ccagaggaat cgacaatgga aaaaatagta aattaagcac
     5881 ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattccac aagcgttgcc
     5941 aataccgaca ccagcgtaat ccacatcatt tcaataaccc gcaatatgaa taaccgaagc
     6001 caaagcggct tggcaaaaaa ggtcgacaac gtattttcac tggagaaaaa cagatcgatt
     6061 tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg agaagcgcag aaaaaacaga
     6121 ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt aaacgttaac
     6181 cactgcccct ctgtcacaaa agaatcactg ttacgcaacg ggctgatagt aagcaggtta
     6241 acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt atattttact
     6301 tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat ccgaaacagc
     6361 agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa cgagggtaga
     6421 agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa tcctgccacc
     6481 agaggttgtg taagaaataa ttctattttg tggttctgct ttttcttaaa ccacgaaaaa
     6541 tcgatattct tcgtgacgca tttataagcc cagtacgcct tgagatcaaa aataaaatta
     6601 tacagcacca gggccagaat cccactaact acaccaatat cctctgatgg aaaagtaaac
     6661 cctgcatttt tccaataaaa gcctatcaga ataagtccaa tagcataaat gtatatcaga
     6721 tttcgtttat cctgaaagcg aataaaagtt aaataatcag gaatcatttg aatacctttg
     6781 catcactaaa tgctttacga caatgcataa ctcgttgctg atccacgcca gcaaaatcat
     6841 ttaacatatt ccccaaatag ggcttattac ttatttcaag gaaagctacc ggggttggca
     6901 tattcagaat atgtatcgag agattcagtc catttcaccg tatgagttaa gtgtaatgag
     6961 agattttctt tgataattga cggagcgacc tcgctttttg ctgttacgtt catcagaacc
     7021 tgatgctctg gcgaagcgat atccagtccg gcaagataat cgcgcattgc ctgaacgccg
     7081 tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga gtttaaccgg ttcgtatcca
     7141 gcggccatca gcagcgtggt aaattcattc aacgcagcct gcgttccccc aataacctgc
     7201 tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc ctgattccgt aatcatcgtc
     7261 tgcagttgtt cgcggttgat gcctttaacc gcctgatctt acccagcaat agtggacacg
     7321 cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAJTNV010000143         7192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNV010000143 DAJTNV010000000
VERSION     DAJTNV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..319)
                     /locus_tag="OO199_003634"
     CDS             complement(<1..319)
                     /locus_tag="OO199_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5537522.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            501..1925
                     /gene="sbcB"
                     /locus_tag="OO199_003635"
     CDS             501..1925
                     /gene="sbcB"
                     /locus_tag="OO199_003635"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5537523.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1968..2195)
                     /locus_tag="OO199_003636"
     CDS             complement(1968..2195)
                     /locus_tag="OO199_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5537524.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2209..3267)
                     /gene="yeeE"
                     /locus_tag="OO199_003637"
     CDS             complement(2209..3267)
                     /gene="yeeE"
                     /locus_tag="OO199_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5537525.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3446..4804)
                     /gene="plaP"
                     /locus_tag="OO199_003638"
     CDS             complement(3446..4804)
                     /gene="plaP"
                     /locus_tag="OO199_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5537526.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4794..4856)
                     /gene="yoeI"
                     /locus_tag="OO199_003639"
     CDS             complement(4794..4856)
                     /gene="yoeI"
                     /locus_tag="OO199_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5537527.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5017..6000)
                     /locus_tag="OO199_003640"
     CDS             complement(5017..6000)
                     /locus_tag="OO199_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5537528.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6046..6870)
                     /locus_tag="OO199_003641"
     CDS             complement(6046..6870)
                     /locus_tag="OO199_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5537529.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6953..>7192)
                     /gene="yoeB"
                     /locus_tag="OO199_003642"
     CDS             complement(6953..>7192)
                     /gene="yoeB"
                     /locus_tag="OO199_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5537530.1"
                     /translation="SEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLK
                     HNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1946 a         1875 c         1688 g         1683 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccaacgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcggcatc agccacttct tgtgggcccg aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatag tttggcacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgct tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataaaca acctaccttc ctctttcacg
      541 attacgaaac ctttggcacg caccccgcgt tagatcgccc tgcacagttc gcagccattc
      601 gcaccgatga cgaattcaat gtcatcggcg aacccgaagt cttttactgc aagcccgcgg
      661 atgactattt accccggcct ggagcagtat taattaccgg tattaccccg caggaggcac
      721 gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt tttaccgtac
      781 cgaagacctg tattctgggc tacaacaatg tgcgtttcga cgacgaagtc acacgcaacg
      841 ttttttatcg taatttctac gatccttacg cctggagctg gcagcatgat aactcgcgct
      901 gggatttact ggatgttatg cgtgcctgtt atgccctgcg cccggaagga ataaactggc
      961 ctgaaaatga tgacggtcta ccgagctttc gccttgagca tttaaccaaa gcgaatggta
     1021 ttgaacatag caacgcccac gatgcgatgg ctgatgtgta cgccactatt gcgatggcga
     1081 aactggtaaa aacgcgtcag ccacgcctgt ttgattatct ctttacccat cgtaataaac
     1141 acaaactgat ggcgttgatt gatgttccgc agatgaaacc cctggctcac gtttccggaa
     1201 tgtttggggc atggcgtggc aataccagct gggtggcacc gctggcgtgg catcctgaaa
     1261 atcgcaatgc cgtaattatg gcggatttgg caggagacat ttcgccatta ctggagctgg
     1321 atatcgacac attgcgcgag cgtttatata ccgcaaaagc cgatcttggc gataacgccg
     1381 ccgttccggt taagctggtg catatcaata aatgtccggt actggcccag gcgaatacgc
     1441 tacgcccgga agatgccgac cgactgggaa ttaatcgtca gcattgcctc gataacctaa
     1501 aaattctgcg tgaaaatccg caagtgcgtg aaaaagtggt agcgatattc gcggaagccg
     1561 aaccgtttac accttcagat aatgtggatg cacagcttta taatggcttt ttcagtgacg
     1621 ccgatcgtgc agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta ccggcactgg
     1681 atatcacttt tgttgataaa cgaattgaaa agttgttgtt caattatcgg gcacgcaact
     1741 tcccggggac gctggattat gccgagcagc aacgctggct ggagcaccgc caccaggtct
     1801 tcacgccaga gtttttgcag ggttatgctg atgaattgca gatgctggta caacaatatg
     1861 ccgatgacaa agagaaagtg gcgctgttaa aagcactttg gcagtacgcg gaagagatcg
     1921 tctaatttga agccgccgcc gctggtacgg cggaggacaa tgttgagtca ggctttttga
     1981 acggtgatgc tccacgctgc atcgccaatc tgctgataat cggtgatggc atgtccttct
     2041 tctgccgccc actgtgggat tgcttcagtt gcctgggtgc aatcaaactc aattaccaac
     2101 tcatcgccgc tggccatttc tgccagtgct gcttttgctt ctatgagtgg aaacggacag
     2161 acctgcgtca ccacatccag ctttttaatc gccatcttta caccttactt aatttgccgc
     2221 agcagttgcc agtcgcgcct tacgctgcgg tcgaacatac acaagccagg acgcagtcca
     2281 gactccgaga atcataaata caaggccaat ccagccctgc caggtcatca ttgcagtcat
     2341 aaccagtcca ttaccgatag agcaaccacc tgcaatactg gcaccgaagc ccatcagtac
     2401 accacctaac ccgctacgta atgttgtttg tgcatcagct gcgcgaacgc ggaactcacg
     2461 gctcgctttg gctgcaataa atgcctccac gaagatccct aacaccagga aaacgcccca
     2521 gttaatgtat ttcacgtcac ccgcgaccag aaattgaaga atattggccg ttggggaagt
     2581 gactcctaac ccaaacatgc gtccggttgc ttcactcagg ggccaggcta aaagcgcaat
     2641 caaaccgatg agtacagcag tgacaaaggg atgccagcgt ttttcaaaca gaatatgagc
     2701 gatcccggtt cggcgcggcg gtaaggtcgc gactttgagt tttggcttct tcaactcttt
     2761 catcaccacc cagagcgtta tcaccagcaa aacggtaacc aacggccaca cagacaaatt
     2821 aaaagtctca gcaatagagt tatgttcagt agtgtagtgc tgcaaggttt gatttaaacc
     2881 actggcgtgt ggagaacgca tcaccgcact catcaccata taagtgaaaa gcgcgatcca
     2941 actgccgatc aatccctcac ccgcgcgata ccaggtcccg gtggcacatc cacccgccag
     3001 aacaattccc agcccgaaaa gatacccacc tataacagta ccaagccacg ggaacgcccc
     3061 ggcttcgtaa gtcagtaggc ccgcctgaat taacgcaaaa acccccacgc tttgtgccga
     3121 aattgcaatc agcaaggcgt aaaacatgcg attatttttc gcgatataca tatcgcgaaa
     3181 accaccagtc aggcaaaaac gcccacgctg catgacaaat cccagcagag caccacaaat
     3241 tagcccgctt aatatcattg aaaacataaa ttaattaacc agatgaatgt taatggggaa
     3301 atttttcatg actggtggaa tgcagaccaa taaccaaatt ctctaaatta gaacaaatgg
     3361 ttattaatga ggatttcgat aaaaaagaat aaaaaaaccg gagccaaatg ttcattcgac
     3421 tccggtttat tattagaaaa gatgattatg ctacgtcttc gtactgcgga accggattac
     3481 ggaagctctt ggtgacgcaa gccaggtaaa tcagaccgat agcagcccag atcagaccca
     3541 gaaccattga gctttcttcc aggttaaccc acagcgcacc aacggtcagc gcaccacaca
     3601 tcggcaggaa cagatactgg aagtgatctt tcagcgtctt gttacgcttc tcacggatcc
     3661 agaactgcga gatgaccgac aggttaacaa aggtgaacgc caccagcgca ccaaagttaa
     3721 tcagcgccgt tgccattacc aggtcgaagt tgattgccag cagggcaatc gcgccaacca
     3781 ggatgatgtt catcgccgga gtacgccatt tcgggtgtac ataaccgaag aagcttttcg
     3841 ggaacacgcc gtcacgaccc attacgtaca tcagacgcgc tacgcctgcg tgcgccgcca
     3901 taccggacgc cagtacggta atggtggaga agatcagcgc gccaacctgg aacgctttac
     3961 ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg atctttaaag cgagagatat
     4021 ccgggaagta cagctgcagg aagtaagttg caaagatgaa gatcatgccg ccaatcagcg
     4081 cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc tttggtttct tccgacagat
     4141 tgctgatgcc gtcaaagccg gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg
     4201 ggataacatg cgcatcgcca gaccagaacg gacgcgtgct cgccagcgta ccagcgcctt
     4261 caccttcaaa tacgccataa acaaccatgc ccagaatcac cgcgatcagc actacctgca
     4321 acacgacgat tacagtgttg acgttcgcta cggatttcag actacgcagg ttaaaggcgg
     4381 tcataaaggc caccagcgcc accacaaaca tccacgatgg aatggaaggc accagagctt
     4441 caaaatagat tttcgccagc agaatgttga tcatcggcgc gaacagatag tcgagcagag
     4501 aagaccaacc caccataaag ccgacagtcg ggctaatgga tttctgggcg taagtgtatg
     4561 cagagccagc agaaggatag cggcgaacca gcttcccgta gctcagagcc gtaaacagga
     4621 tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc atccgtgagg cctgaaacga
     4681 taccaaatgt atcaaacagc gtcatcggct gcatataggc tagacccatc attacaaccg
     4741 gaactaacgt aagcgtttta cgcaattcca cgcgagaggt gtttggagta acgttatgcg
     4801 acatggttat tctcctttac ggtgataacc gtcgcgtaag caaaaaattg ccccattttt
     4861 ttggattcct cagcgacaac aactgtcgat ttttagtaaa tatctatccg gtacgaagcc
     4921 cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata accgacgcta attaaacgtc
     4981 ggtttttaat cattttttga cagcggcgca ttgtgcctaa atggggggga aatgacaaga
     5041 gaatgagagg cttgtcataa taatttttct ttgaatggct gattttccgt catcagattt
     5101 tgcataaaca ccggcgatcg cactatttgc taaaatttca tctcgccacc agccgctggc
     5161 aagccctgcg gtctgttcat tccagccaat ccagacaggt tcaaagagag tttgggcgac
     5221 cactttcttc tcgattaacg cgccactatc gagaaaacgt tgcgccagat accgtggtaa
     5281 atagccgcag ccaaggccgc taatttgcag ctccagtttg gttttaaaat cgaaaacggt
     5341 aatggcttct tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc
     5401 ccccaccaca atcgcccgat atcgtttaat gatacgacgg tttaacggtt cttcttcctg
     5461 ggctaacgga tgatgaggcg cgaccgcaaa gacttgttca agatcgccca accgcgagaa
     5521 accaaactca ctggaagatg gtggctcatg catcgcgcca acgataatat ccgctcgccc
     5581 ctgagtcagg gcatcccagg aaccaccgag tacgccattg ataaacttca ggcgcgtcac
     5641 gctatgatgt tgataaaaag cttcaatgag aggcgcaagc agagaaaaag gaaacgtgtc
     5701 gtccacgcca atcaccagct cgttttccca gccttcatgc agtttgatcg cctgcttttc
     5761 cagttctcgc acggtatgca gaacttcccg ccctttctct aataacattt ttccggtgcg
     5821 ggtgaattta gcgcggtgtc cgctacgatc aagcaattga atatttaggt cgctttccag
     5881 cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag agtttggctg acgccgccgc
     5941 aaaactgccc tctttttcta tggcatcgag gatcatcaac acatccagca gtggtttcat
     6001 acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact ccaacggcat
     6061 taccagcgga tcgggatact ggtattcaaa tcccagttca ttacaaatcc gactgccatc
     6121 aataatctta cctttgccgc tgtccagact atttctgaac tgcggaggtt gcagccccag
     6181 taaacgggcc atctgcggat agaaaacatt acgcgcaggg tgagctggcg cacatatatt
     6241 atagatgtgt ccgcctttag gtgcctgtaa caacagagtg atagcgccaa taacatcttc
     6301 taaatggact aaattaacac catgttcacc atcaggggcg gtttttccgg caaagaagcg
     6361 tcccggatga cgtcccggtc cgaccaggcc cgcaagacgc agaatatcga ccgaagtacc
     6421 gggtaaattg tgcagccagt cttcgagttc ttctaatact cgtccactgt tggttaccgg
     6481 attacgcggg gtagtttctt taaccgtgcc ttgcgcgtcg ccatagacag atgttgagct
     6541 ggtaaaaata attcggggaa tacgatgggc cagcgcgcta tccactaact cttgtaccgc
     6601 ttgtaaatag aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc
     6661 atcggcatcc ataagggcat ccagatcgtc agaatcgcaa actaactcag gctccatgcg
     6721 aagcagatag ctatcaatgc cactcattcg ggccgcttcg acaccatctt gtgtggtttt
     6781 actcccggtg acttgccagc ctcgcgctga aagtgacatc gccagcggca tacctaacca
     6841 ccctaacccg acaattgcga cctttttcat ccgttatctc ctgatacacc ttagatctat
     6901 aaggctacgc tagcgtatca aaactgacaa ttcattctat gaatgaatct gttcaataat
     6961 gataacgaca tgctgcaatg agcagtgaat catcagtaac cgcgtatacc agacggtgct
     7021 cctctgtaat gcgtcgggac cagaaacctg acaaattatg tttcaggggt tctggtttcc
     7081 ccttaccttc aaatggtgtt ctgcgggtat ctttgataag ttcattgatc tttttaacaa
     7141 ttcgcttatc tgtttcctgc cagtacagat aatcatccca tgattcctca ga
//
LOCUS       DAJTNV010000144         6979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTNV010000144 DAJTNV010000000
VERSION     DAJTNV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(102..1004)
                     /locus_tag="OO199_003643"
     CDS             complement(102..1004)
                     /locus_tag="OO199_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5537531.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1083..2036)
                     /gene="lsrR"
                     /locus_tag="OO199_003644"
     CDS             complement(1083..2036)
                     /gene="lsrR"
                     /locus_tag="OO199_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5537532.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2285..3820
                     /gene="lsrA"
                     /locus_tag="OO199_003645"
     CDS             2285..3820
                     /gene="lsrA"
                     /locus_tag="OO199_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5537533.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3814..4842
                     /gene="lsrC"
                     /locus_tag="OO199_003646"
     CDS             3814..4842
                     /gene="lsrC"
                     /locus_tag="OO199_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5537534.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4842..5834
                     /gene="lsrD"
                     /locus_tag="OO199_003647"
     CDS             4842..5834
                     /gene="lsrD"
                     /locus_tag="OO199_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5537535.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5846..6869
                     /gene="lsrB"
                     /locus_tag="OO199_003648"
                     /pseudo
     CDS             5846..6869
                     /gene="lsrB"
                     /locus_tag="OO199_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1611 a         1765 c         1832 g         1771 t
ORIGIN      
        1 gtaatattgt agtagctaaa ttgagatgtc cgtctctgac taaatagaca tgtccgcgct
       61 acagtctcac cacagcgatc actgctttca aaggatggga ctcatgacgg catacggaac
      121 ggagtaatcg gcaactgatg ccgggcctcg ctgagcgtgc actgcttatt atccgtgaac
      181 gtgccgccaa gaaccgcggt ttgtgccgga cgcacaacgc ctaacccaag gcaaccaagc
      241 tgcacgtcgc ctcctccaac gaccaccgga gtgcccgcct tcagaccgca gagttccgcc
      301 gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt tgacaggaga aagaatatcg
      361 gcacgcaggc cagccatatc cagcaatgca ggtttccagg cacgggtggt tagatcaaga
      421 agtcccgtgg tgccagcgtt agagggatcc accgccagtt cgccgctgag catataggcc
      481 agccagtcgc tgatcatggt gatggttgat gcctgacggt aaatatcgga acgatggtgc
      541 gccagccaaa gtaatctggg gatggcactt aaagccagtg tttgtccggt cgctcgataa
      601 acttcgtttt caaaggtatt gttgtgcagt tctttaagtt cgctaacttc gcgtgccgct
      661 ctggcatcca cattggcgca ggcccagatc ggtgttcctt cattgttata taaaacaatg
      721 ccttcacgca tagaacatgc cgaaacggca gcgatatact ccgggtctat gccggcgttg
      781 tgcagcgcct ggcgcataca ctcacacgcc agttgccagt ttttgttgag atcaaattcc
      841 atagaaccag gaacgtccgg tactgccaga tgccgccact ccgcctgtcc cactgctatt
      901 tgattgcctt ccaggtcgaa tatcacagcc cgaatacttc cggtgcctgc atccagcgcc
      961 atcaggtagt actttgattc tgaaggggta aagagtcgag ccattagatt atcctcggct
     1021 tatgcgttct gtctgttcct ctatacgttc tccatcattc ccggtaataa ggtcatgcaa
     1081 atttaactac gtaaaatcgc cgctgctgtg tcctgatcgg taaccagtgc gttgatataa
     1141 ccgcctttca ttgcagcggc aattgcttcg gctttatttt ctccccctgc cacgccaacc
     1201 cggacgggta tggtcttcag cgcgcttaaa ggtaagccaa tcagttcgtt atgtattttg
     1261 atatccgtga caacgtcacc ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc
     1321 ccttttcggc caatcattaa ctgttcgccc tggctgatat aaccggagcg aatgattgtc
     1381 gcgtcgtctt gttgactcac ggctccaatg ccgacaatcg ccacatccgc tgcttgcgcg
     1441 gctagcagaa catctttgac gcaattttca ttttttagcg tacgggcaat gtcagcggag
     1501 gatgcccgca acggagccgg aataatattc acactgcacg ccgcgttaag ctgcccgatg
     1561 cccgtcatat aagaaccgac gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa
     1621 ataaaaccac ttaagcgttg cagcgtattc atggttgcct cgccaaaacc aatcgccagc
     1681 atctgttgtg gttgaagtaa actcatcaac atatgcgccg cgcctatccc tagtcgcccg
     1741 ccgacatcag catccgcaag cccagggatc acccggacat gttgcagcga aaactgacga
     1801 cgtaattgag tttcatattc cagacagcct tcaaagcgag aattaatctg tacgcgaata
     1861 atgccggact gatgcccttt ctccagcaag cgcgacactt tcaaacgggt caggccgaga
     1921 cgatcgctga tctcgctctg ggtcagcccg tcgtgatagt aaaaccacgc gatccgcgcg
     1981 acctgttctt cttcacacat tccctgttct gaaattgccg aatcgttgat tgtcataatt
     2041 cattcttcac tttgaacata tttaaatctt taatgcaatt gttcagttct tgcgcattta
     2101 tatctgtgat ggcaaccaca gtttgactct acgagcatga acaaacgcaa ccgtgaaaat
     2161 caaaatagca taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta
     2221 tgaacaaatt aaaagtagaa atacatttgt tcaaaactca cctgcaaaac tgaacggggg
     2281 aaatatgcaa acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa
     2341 acagtattca ggggtcaatg tcctgaaagg catcgatttt acgttgcatc agggggaggt
     2401 ccacgccctg ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg
     2461 tattacccct gctgatagcg gtacgctgga gattgggggc aacaactacg ccagattaac
     2521 gccagttcat gctcatcagc tgggcattta tctcgttccc caggaaccgc tgcttttccc
     2581 aagcctgtcg ataaaagaaa acatcctgtt tgggctggca aaaaaacagc tctccatgca
     2641 gaaaatgaag aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg
     2701 atcgctggat gtcgccgatc gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc
     2761 gcggattctg atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt
     2821 gtttagtcgc ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa
     2881 gctgccggaa attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcga
     2941 cttaagcggc aaaaccagcg aactgtctac cgacgacatt attcaggcca tcaccccagc
     3001 ggtacgggaa aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggtaaccg
     3061 cccacaacat gccgtcggaa cgccggtgct gacactggaa aatctgactg gcgaaggctt
     3121 caggaatgtc agcctgacgc tcaatgccgg agaaattctg ggcctggctg ggctggtggg
     3181 agccggacgc acagaactgg ccgagacgct ctatggtctg cgtactttgc gtggcggacg
     3241 cattatgctg aatggtaaag agatcaataa attatccact ggagaacgtt tactgcgcgg
     3301 tctggtttat ctgccggaag atcgccagtc atccggactg aatctcgatg cttcgctggc
     3361 ctggaacgtc tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga
     3421 taatgccact ctggaacgtt atcgtcgggc gctgaatatt aaatttaacc aaccggaaca
     3481 agctgcacgg acattatccg gtagcaacca gcaaaaaatc ctcattgcca aatgcctgga
     3541 agcttcgccg caagcattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg
     3601 taatgatatc taccagctgt tgcgcagcat cgccgcacag aatgtggctg tgctgtttat
     3661 ctcctctgat atggaagaga tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg
     3721 cgaaattacc cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt
     3781 tgccttcggc gatagtcagc gtcaggaggc atcatgctga agtttattca gaacaaccgt
     3841 gaaatcacgg cattgctggc ggtggtgctg ctgtttgtat tacccggttt tctcgaccgc
     3901 cagtatttaa gtgtgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg
     3961 ctggcaatgg gcgcgacgct ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg
     4021 attaccggaa tgtgcgcggt gctgttgggg atgttactga acgcaggata ttcactacct
     4081 gttgcctgtg tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgccctg
     4141 gtcgcgtggc taaagatccc tgccattgtt gccacccttg gcacgttagg gttgtacaga
     4201 ggcatcatgt tgctgtggac tggcggtaaa tggattgaag ggttacccgc cgaactgaaa
     4261 cagctctccg ccccgctgct gcttggcatt tcagcaattg gttggttgac gataattctg
     4321 gtggcattta tggcctggct gctggcgaag acggcgtttg gacgcagttt ttatgccacg
     4381 ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg
     4441 gcattttcgt tgaacggctg catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt
     4501 ggttttatcc tcaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg
     4561 ctgggcggca ttagtttgct cggtggttcc ggtgcgttca tcggtgcggt actcggcgca
     4621 tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc atggtggaat
     4681 gattttatcg cgggtctggt tctgctggcg gtgctggtgt ttgatggacg cctgcgttgt
     4741 gctctggaac gtaatctacg gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc
     4801 gttaaacccg cttcgtcagg taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt
     4861 gggaactggc tcttgccgca ctgctcgtta ttgagattgt cgcatttggt gcaattaacc
     4921 cgcgaatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca
     4981 ttgtcgctct accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt
     5041 cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc
     5101 cgctggcgat actcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat
     5161 taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg
     5221 ccggaagcgc tctgctgctt tccggtatgg ccggagcgac ggggtacgaa ggtattggtg
     5281 gattcccgat ggcgtttaca gatttcgcta acctggatgt gctgggactc cccgttccgc
     5341 tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg
     5401 gacgtaatgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc gcgattccag
     5461 ttaaccgcac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg
     5521 tgctgctggt atcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc
     5581 ccgccatcac cgccgtggtg cttggcgggg ccaatattta tggtggttcc ggttccatta
     5641 tcggcaccgc cattgcggtt ttattagtgg gatatttgca gcaaggtttg caaatggcag
     5701 gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct gcttatcgtc gttgtcgtag
     5761 gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata
     5821 acccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact
     5881 tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt
     5941 tattcccaaa ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc
     6001 gggtaaagag ctgagcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg
     6061 tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta ttcgtttctg
     6121 cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc catgcaacgt ggtgtgagag
     6181 tgctgacctg ggactctgat actaaaccgg agtgccgctc ttactacatt aatcagggaa
     6241 cgcccgccca gttgggaggt atgttggtgg atatggcggc gcgtcaggtg aataaagaca
     6301 aagccaaagt cgcgtttttc tactcaagcc ccaccgttac ggaccaaaac cagtgggtga
     6361 aagaagcgaa agcgaaaatc gccaaagagc atccaggctg ggaaattgtc actacgcagt
     6421 ttggctataa cgatgccact aaatcgttac aaaccgcaga aggaatatta aaagcgtata
     6481 gcgatctcga cgccattatc gcccccgatg ccaacgccct gcccgctgcc gcacaagccg
     6541 cagaaaacct gaaaaatgac aaagtagcga ttgtcggatt cagtacgcca aatgtgatgc
     6601 gcccgtatgt agagcgcggc acggtgaaag aatttggact gtgggatgtg gttcagcaag
     6661 gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa aggatcaatg aaaacgggcg
     6721 acaagctgga tatccagggc gtaggtcagg ttgaagtctc gccaaacagc gttcagggct
     6781 atgactacga agcggatggt aatggcatcg tactgttacc ggagcgcgtg atattcaaca
     6841 aagagaatat cggcaaatac gatttctgat gtgcattact taaccggagt aagttatggc
     6901 agatttagac gatattaaag atggtaaaga ttttcgtacc gatcaaccgc aacaaaatat
     6961 cccttttacc ctgaaaggt
//
LOCUS       DAJTNV010000145         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNV010000145 DAJTNV010000000
VERSION     DAJTNV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO199_003649"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO199_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5537536.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO199_003650"
     CDS             2089..2238
                     /locus_tag="OO199_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537537.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OO199_003651"
     CDS             complement(3005..3445)
                     /locus_tag="OO199_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5537538.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OO199_003652"
     CDS             3663..4052
                     /locus_tag="OO199_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537539.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OO199_003653"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OO199_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OO199_003654"
     CDS             complement(4607..5443)
                     /locus_tag="OO199_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5537540.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="OO199_003655"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="OO199_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /locus_tag="OO199_003656"
     CDS             complement(5793..6266)
                     /locus_tag="OO199_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5537541.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="OO199_003657"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="OO199_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1759 c         1771 g         1675 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt ctcatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAJTNV010000146         6611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNV010000146 DAJTNV010000000
VERSION     DAJTNV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(94..2097)
                     /locus_tag="OO199_003658"
     CDS             complement(94..2097)
                     /locus_tag="OO199_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537542.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2094..3230)
                     /locus_tag="OO199_003659"
     CDS             complement(2094..3230)
                     /locus_tag="OO199_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5537543.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3223..5502)
                     /locus_tag="OO199_003660"
     CDS             complement(3223..5502)
                     /locus_tag="OO199_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537544.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5513..6601)
                     /locus_tag="OO199_003661"
     CDS             complement(5513..6601)
                     /locus_tag="OO199_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5537545.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1510 a         1821 c         1777 g         1503 t
ORIGIN      
        1 ggtgactctc accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga
       61 actggagtag aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa
      121 taattgttcg caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt
      181 attacgaaat tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt
      241 cgccagcaac tctgcctgat taatagcgct ctgttcctga tagcgccagc cgttatggag
      301 cagttgaata agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg
      361 cagaacgctg gcagaagata gtgataccac cacaggcgca gtttctgcgt ccagcgccca
      421 ggcacgagtc tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact
      481 gacaaagcca gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg
      541 ttcaatggca taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc
      601 accttcgccg ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga
      661 aatacactca gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca
      721 ggcgggggga cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg
      781 cgcgctcaac atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg
      841 ggtagctaac agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc
      901 acccgccagt gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc
      961 ctgttcactt cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt
     1021 aaaccagatg cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac
     1081 cagccgcaga tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca
     1141 cggcacagga ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa
     1201 tcgactgttt aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg
     1261 ggcgtggaag gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca
     1321 gctgcagcgt tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg
     1381 ctggctgatg ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt
     1441 tttgccttct tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg
     1501 ccagattaaa tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc
     1561 cagtacgaca tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact
     1621 gctgcgggaa taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg
     1681 cgcacagaac aactcaatgg tgatgctttt agcgaccagc gcctgtgcgc gtttgcgggt
     1741 ggcatcggga agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt
     1801 tcccgtaggc tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg
     1861 acggcacatg ccgctggccc cataggtgca ctgagcctct ttcagtgcct ggccgttcgc
     1921 cagctgggta cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat
     1981 ttccgggacg ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct
     2041 taacgccgtc agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac
     2101 tgaagattct ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg
     2161 ccgacattaa tcagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg
     2221 agcgctgcca gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc
     2281 agcaatgatg atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca
     2341 ggttgttcaa taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct
     2401 aattgcactt tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg
     2461 cttgtgtcaa acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc
     2521 atcaccgcag agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc
     2581 agttgccatt gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac
     2641 aacttgccgt gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca
     2701 aagtttcgcg ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc
     2761 tgacgaactt cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg
     2821 cgggcggcag ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc
     2881 aggctttcgg aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg
     2941 ccgtagcgca gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg
     3001 tgaatactgt tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt
     3061 tcaccacgct ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc
     3121 tgccgggcgt tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat
     3181 cgccagcgtt gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc
     3241 ccagtgttgc agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg
     3301 cggtggacaa tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg
     3361 ctcaagcacc tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc
     3421 gcgtaaatcg ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct
     3481 gaaaccggcg ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc
     3541 gtgcagcgcc tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc
     3601 ggcactagcg ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag
     3661 tgccaggaca tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga
     3721 aacgccgggg agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca
     3781 gctatgcaag tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca
     3841 cagcgtgcga tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc
     3901 gctaatttca tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc
     3961 aaattgattt tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga
     4021 cgccagaccc gctgacgccg cttggctaag acatgtggta atgctgccga ttccacccgc
     4081 gtccagcata tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca
     4141 ggcggcctca atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc
     4201 accgttgcct gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag
     4261 tattgccagc cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa
     4321 ggtaagctcc gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg
     4381 cagaggcggt tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc
     4441 tatcaacgtt agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg
     4501 caacggcgca ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt
     4561 gcgtaacggt agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc
     4621 agccatatcc gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt
     4681 tagcagttgt tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg
     4741 ggcaggcatt cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggt
     4801 gagataacaa cctgtaacgg catctgccag cgacggcaat tctggcgtgt taattacctg
     4861 cggacattcg cgccacattt ttgccagtgc cggagcgtgc cagccaccgc agacaactaa
     4921 cacgtcgcca ttattttgct gcatcgccca tccaatccag cgggccataa aggcttcgcg
     4981 ctgacgattt agagcatcgc cgggggagtc gccccgcagt tgggcaaaat agtgcgccag
     5041 tgcagagggt aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtgtc
     5101 gctgttatcc atgcgggtgg cacgcagcag caaggtctgg ctttcctctt gcgtatcagg
     5161 cgaatcgtcc tcttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc
     5221 ctgaatacga cgatcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt
     5281 ccaggcacca cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac
     5341 cggaagctgg tgggctaaaa acagttcgtc tacccggtca ttaaaatcag cagggccttc
     5401 aatcaacacg tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc
     5461 cggactatga tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca
     5521 ggcggtggcg ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgacacgct
     5581 gttcgaagta acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg
     5641 caatgcagtc caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga
     5701 cgcctacggc gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat
     5761 ccattgacgt tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgg accagtaact
     5821 ccagtaccgc gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag
     5881 cactggcgac aagctccagc tcctgggcga aatccataat cggaaagaca gtttcaaaat
     5941 cgaagcgacg ctttagcgca gcactcattt cattgacgcc gcgatcgcgg gtattggcag
     6001 tggcaataat attaaagcct tctcgcgcat agagctgact ggcttcacca gtgagttccg
     6061 gcaccgtcat caccctgtca gaaagcatac ctaacagaca gtcttgcact tccagcggtg
     6121 tacgcgttat ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg
     6181 cagggaccag cgcttccgtt gatggaccat gattgataag cagggcgtag ttccagccgt
     6241 acttgatttg atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc
     6301 taatagaggt tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca
     6361 acatcaaacc gcgtccggtg gcaagagtga ctaacatacg ttcgacagcg gagggactaa
     6421 tcaccacttt acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag
     6481 gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc attttgtttt aattttgcca
     6541 gttcatcggc gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca
     6601 tatattcatc c
//
LOCUS       DAJTNV010000147         6508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNV010000147 DAJTNV010000000
VERSION     DAJTNV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..183)
                     /locus_tag="OO199_003662"
     CDS             complement(<1..183)
                     /locus_tag="OO199_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR5537546.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAI"
     gene            complement(180..1058)
                     /gene="xdhB"
                     /locus_tag="OO199_003663"
     CDS             complement(180..1058)
                     /gene="xdhB"
                     /locus_tag="OO199_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5537547.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1069..3366)
                     /gene="xdhA"
                     /locus_tag="OO199_003664"
     CDS             complement(1069..3366)
                     /gene="xdhA"
                     /locus_tag="OO199_003664"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5537548.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            3781..4536
                     /locus_tag="OO199_003665"
     CDS             3781..4536
                     /locus_tag="OO199_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5537549.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4615..4688
                     /locus_tag="OO199_003666"
     tRNA            4615..4688
                     /locus_tag="OO199_003666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4647..4649,aa:Gly,seq:ccc)
     gene            4803..6407
                     /locus_tag="OO199_003667"
     CDS             4803..6407
                     /locus_tag="OO199_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5537550.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT         1627 a         1586 c         1714 g         1581 t
ORIGIN      
        1 tattgctgtg ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg
       61 tttgacactt agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc
      121 gtgaagctga aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt
      181 cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc
      241 tggatgagat gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct
      301 tgcaggacag attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca
      361 gtctgttcgg catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt
      421 aattcgctga aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata
      481 tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt
      541 ggcggaaaat gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg
      601 gtgtgaaagc cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc
      661 gcgtcataaa ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt
      721 ccaccgtagg tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat
      781 aacgccggga gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca
      841 gagccgatac gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta
      901 tgaatatcaa caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca
      961 gtgccaccgg cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca
     1021 agggttgctg cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc
     1081 tgccagatgg aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac
     1141 accggtagcc atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc
     1201 acccagtgac ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc
     1261 cagttgtggc agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac
     1321 cacgccgctt ttagcatcga tgatcatctc ttcaaatagc gcccagtcaa tgcccattcc
     1381 cattccgccg tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg
     1441 aacgttgagg atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac
     1501 aaaggtacag ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag
     1561 ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa
     1621 cggctcttcc ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc
     1681 tgactgatgt agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc
     1741 actgcgcagc gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa
     1801 cggcgtaacg tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac
     1861 ggtttctgcc accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc
     1921 gctttgcacg ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac
     1981 gccgacaggc caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa
     2041 attgccttgc tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc
     2101 tttttcaaga cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc
     2161 atctccttcg cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt
     2221 cgcggcgtca tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg
     2281 catcgcacca gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca
     2341 acgaggataa aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga
     2401 tgcataagcg ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg
     2461 gttcacgccc atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca
     2521 ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc
     2581 cgccattggc tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt
     2641 gatgactcgt acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat
     2701 gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag
     2761 cgatgttacg ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac
     2821 ctggtagtcg gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt
     2881 cagtaaattg ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt
     2941 ggtgataacg ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt
     3001 gagttcatcg cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt
     3061 tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc
     3121 tgtagcgaat ggaatatcag gcacatcctc ccaagtaaaa atcgccagta cgcccggtaa
     3181 acttctggct tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac
     3241 atatttcgcg taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt
     3301 gaccttagca atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc
     3361 ttccatagaa atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc
     3421 aggcaagatt tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg
     3481 ttaaaaaaac acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca
     3541 atatgagtgg cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa
     3601 tagggtaaaa agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc
     3661 cctttgccaa atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat
     3721 tgggtatcaa gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt
     3781 ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga
     3841 ctgctattgg cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc
     3901 gtttacaccg tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc
     3961 gtaaaagagc tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag
     4021 aaactaaaac tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca
     4081 acgactaaaa ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca
     4141 gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca
     4201 gcagatggcg gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc
     4261 gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc
     4321 atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta
     4381 aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg
     4441 gcatctgttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt
     4501 tacttgccgc cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca
     4561 gcgcggcact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc
     4621 gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgt
     4681 ccgctccatg acctttctta aagtactcca acctactgaa tcctaaggga tatggcttta
     4741 agcctccttg cagagttact tactgtaacc cactgtgtaa ccctctcagg gcatacagac
     4801 atatgattca gctatccagt agtcacaaac ttccagcagt ctactatctc taccagcgta
     4861 acggcgtcta ctacttccgt cttcgggtca gacaatcaaa caatgataga atgacctcag
     4921 tatcattacg gaccaaggat agacgcaccg ctatggctca ctcaaggcac atcaaggcag
     4981 cactcaaggc aatacacgca gacagaccca acgctaccta tgaggagatg agggagcacc
     5041 ttaaggacat cgctgagtgt gaactgtcta tgggccgttc agatctcttt gagcctgaca
     5101 tgagggatat ctaccgggac caatacggag aacttggtga gagccttacc gatgctctgg
     5161 ctagtgagcc gctaagtatt gaccagcatc gttacatcaa tgaggccctg aaggtgctaa
     5221 aagcctgtat gaggcgtatc gaagcgggag atagtcagcc tctgatagat tacgtcgatc
     5281 gttttaatga tattgatcgg caagatgacc aagcagactc tgtgtcccta tctgttagtg
     5341 ctccagaagt aaaaccagaa gttacgccta gcattaccat agcgagcctc tttgagcaat
     5401 acgaagcgga gaactctcag aactggaagc ctgcaacctt aagggaaaac aaagcgtcac
     5461 acgctgcgtt aatcgaaata ttcgaccatc tgggtctcaa tgcagacgct aacagggctg
     5521 acatgcttaa ggtccgtgat gtgctccagc agttacctaa gaaccgtaag cagcgcttta
     5581 aggatgcccc acttgctgac ctgttgagcc gtgaggataa gacagattgt ctggatgtcg
     5641 ttaccatcaa caacaagtat cttattaaga tggctgctgt ctttaggtgg gctgtgcgca
     5701 atgacttgat taaaaagaac atgactgagg gtctggagct taaagtccct cagcgtaaag
     5761 cctcaggggc ccgtaacgcc ttctctacgg agcaggtggg gcaactactg gtcgcagcta
     5821 aggcatactc tcagaagacc tctggtaagc cctaccacta ttacgttacg gctttggctg
     5881 ctatcactgg ggcgagactc aatgaaatag cccaactaca ggtcaaagac gtcaggacca
     5941 ctgaggcggg aaccgtttat attcatatca atgaagacga tagtagtcta cccggtaaga
     6001 gcatcaagaa cgctcatagt gaccgctgtg tgcctctggt cgatggggcc tatggtttta
     6061 tattggctga ctttatggcg cttgtagaga cccgtagagg cgctgacggt gatgacgcta
     6121 tggtgttcga tggtctgcgt ctcatgaaga acggttacgg tgagcaggtg agtaaatggt
     6181 ttaatcggac gttgttgcct aaagtcctcg tagaccgtag cggcttagcg tttcactcgt
     6241 ttagacatac agttgcgacc cagttaaaac aacatggggt cgagttggcg tatgcacagg
     6301 cgatcatggg gcatagttca gggtctatta cgtacgaccg ttatgctaaa gaggtcgagg
     6361 ttgataggct ggtcaatgtg atggctgatg tttataagga gacctagagg agaccatagt
     6421 ctaggatgac acttactaca gacctgtgtg gtcatgtttt tgactcatgg ttttctctac
     6481 ttgtcaaggg ataattaata cgactcac
//
LOCUS       DAJTNV010000148         6491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNV010000148 DAJTNV010000000
VERSION     DAJTNV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..528
                     /gene="glnH"
                     /locus_tag="OO199_003668"
     CDS             <1..528
                     /gene="glnH"
                     /locus_tag="OO199_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5537551.1"
                     /translation="GIIPALQTKNVDLALAGITITDERKKAIDFSDGYYKSGLLVMVK
                     ANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPNIDNAYMELGTNRADA
                     VLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDELRDKVNGALKTLRENG
                     TYNEIYKKWFSTEPK"
     gene            667..1326
                     /gene="glnP"
                     /locus_tag="OO199_003669"
     CDS             667..1326
                     /gene="glnP"
                     /locus_tag="OO199_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5537552.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            1323..2045
                     /gene="glnQ"
                     /locus_tag="OO199_003670"
     CDS             1323..2045
                     /gene="glnQ"
                     /locus_tag="OO199_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5537553.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            2306..4530
                     /gene="ybiO"
                     /locus_tag="OO199_003671"
                     /pseudo
     CDS             2306..4530
                     /gene="ybiO"
                     /locus_tag="OO199_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(4527..5453)
                     /gene="rlmF"
                     /locus_tag="OO199_003672"
     CDS             complement(4527..5453)
                     /gene="rlmF"
                     /locus_tag="OO199_003672"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5537554.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            5729..5989
                     /locus_tag="OO199_003673"
     CDS             5729..5989
                     /locus_tag="OO199_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5537555.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            6254..>6491
                     /locus_tag="OO199_003674"
     CDS             6254..>6491
                     /locus_tag="OO199_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537556.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVIS"
BASE COUNT         1528 a         1703 c         1669 g         1591 t
ORIGIN      
        1 gggatcattc cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc
       61 accgacgagc gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta
      121 gtgatggtga aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg
      181 gttgctgtga agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa
      241 gatctgcgtc agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca
      301 gacgccgttc tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt
      361 cagttcaaag cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa
      421 ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga
      481 acttacaacg aaatctacaa aaaatggttc agtactgaac cgaaataata acgctacacc
      541 tgtaaagcgc actggcagtt ccctctcccc cctggggaga ggattagggt gaggggcgca
      601 aacccgctcc ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa
      661 caacatatgc agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt
      721 gccaaaatga ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg
      781 ctggcaggtt ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt
      841 attgaagtga tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg
      901 ccgatggcgt ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg
      961 atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa
     1021 ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc
     1081 attttaccgc tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc
     1141 attaaagaca cctcgctgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa
     1201 gaaattattg ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat
     1261 ctgattatta ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc
     1321 ctgtgattga atttaaaaac gtctccaagc actttggccc aacccaggtg ctgcacaata
     1381 tcgatttgaa cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta
     1441 aatcgaccct gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg
     1501 tcgatggcct gaaggtcaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag
     1561 gtatggtgtt ccagcagttt tacctcttcc cgcatctgac ggcgctggga aacgtcatgt
     1621 ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc
     1681 tgctggcgaa agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg
     1741 gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt
     1801 ttgatgaacc gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc
     1861 aggatctggc tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg
     1921 agaaagtagc ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc
     1981 cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct
     2041 cttaataaga cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac
     2101 ttattaaaat tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa
     2161 agcccgcacg ttgtcagcaa cctgcttaat atcccttcct ccctttcacc cgaaagggag
     2221 gcacaccaga ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt
     2281 gctctgtttt ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct
     2341 gggcgcacct gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc
     2401 aacgactgaa ccggccccgg aaccggatat cgaacaaaaa aagcggccta tggcgcactg
     2461 gcggatgtgc tggataacga cacctcgcgt aaagagttga tcgaccagtt gcgtactgtc
     2521 gccgcgacgc cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag
     2581 caaaccgtgc tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc
     2641 cgcttcgggc aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa
     2701 accttcagca atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac
     2761 tggctgattc gcctgtgcgc actgccgcta tatcgcaaaa tgggtcagtg ggcgaggcaa
     2821 aaaaatcgtg agcgcagcaa ctggttgcag cttccggcga tgattattgg ggcgtttatt
     2881 atcgacctgc tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg
     2941 aatgcaggca gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg
     3001 attgaatttt tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc
     3061 ccgttcacga ttcaggacga gagcgcccgt tactggggtc gtcgcctgag ctggttaagc
     3121 agcctgattg gctatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta
     3181 cagataggtg cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac
     3241 ctgatcttcc gaaataaaaa agagattacc cagcatatgc tcaactttgc ggagcattcg
     3301 ctggcttttt tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc
     3361 gcctatttta tcatgctgtt tttcttttcg ttgttcgatc cgggcaacag tctgaaattt
     3421 atgatgggtg caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt
     3481 atgttttccc gctggctggc gaaaaccatc accctctcgc cacatactca gcgtaactat
     3541 ccggagctac aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt
     3601 ctgaccgtct gcgtggcagt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg
     3661 aactggctgc aaaacggcgc ggggcagaaa accgtagata tcctgatccg catcgcactc
     3721 attcttttct tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg
     3781 gcttcggata ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg
     3841 ttccgtaacg cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa
     3901 atcggcgtca atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg
     3961 tttggttcgc aaacgctggt aaaagatatt atcaccgggg tatttattca gtttgaaaac
     4021 ggcatgaaca ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg
     4081 tcgattcgct ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct
     4141 tcgataacca cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat
     4201 gttgatcgcc atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtaacggaa
     4261 ttgatggaaa acgaagaaat tcgcgggctg attattggtg aaccgaactt tgccgggatt
     4321 gtcggcttaa gcaataccgc gtttacactg catgtttcgt tcaccacgct gccactcaaa
     4381 cagtggacgg tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc
     4441 gttcgcgcgc cagtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa
     4501 ccgctgccgc cgggggagcc aacgctttaa cgttggcgat taacaaaacg gcggcgctgc
     4561 tcgtcgttca taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct
     4621 ttttttacca ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt
     4681 tcaccacgag ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct
     4741 tcaatcatct ttttgataaa gttgacttca ccgccttcac accacaactc ctgttgctga
     4801 ccaccaaagt tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta
     4861 cctgcccgcg ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat
     4921 tgctcgtttt tgtggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga
     4981 cgaatggcgc ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc
     5041 tggctgctgg tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga
     5101 taaatacagt tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg
     5161 cttgcttcag ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt
     5221 gggcagagaa aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac
     5281 gccttattga gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctcccctgcg
     5341 ggtgtaagcg tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg
     5401 tagcggctgt gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc
     5461 tcctttaagc agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt
     5521 ttatcattcc ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag
     5581 cattatgcat gtcattgatt tgtcttattt tgtaaaaata tatcgttcaa aaaataagca
     5641 tatctctgtc aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac
     5701 tacccgttgt atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac
     5761 cgtcctgagc ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag
     5821 cggtcagcta cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct
     5881 ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc
     5941 cgcaggcgga aatgaccaga tgttaggtac tgcaaccatc tacaaataac cccaccaacg
     6001 taactgcatt cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata
     6061 tgaacgcaat gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcatc
     6121 aagtgataat gcttatcaaa attactatca cttgcacgaa cactatcacg ggattaacag
     6181 tggcatcgca tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa
     6241 agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac
     6301 gttctttgcc ggtctttgta ttggtattac gcctgtggca caggcactcg ccgccgaagg
     6361 gcaagctaac gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc
     6421 acaacaatct gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac
     6481 ggtgatttct g
//
LOCUS       DAJTNV010000149         6402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNV010000149 DAJTNV010000000
VERSION     DAJTNV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO199_003675"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO199_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5537557.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO199_003676"
     CDS             complement(1433..1759)
                     /locus_tag="OO199_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5537558.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO199_003677"
     CDS             1894..2235
                     /locus_tag="OO199_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5537559.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO199_003678"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO199_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO199_003679"
     CDS             complement(2833..3543)
                     /locus_tag="OO199_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5537560.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO199_003680"
     CDS             3651..3956
                     /locus_tag="OO199_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5537561.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OO199_003681"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OO199_003681"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="OO199_003682"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="OO199_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1500 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tg
//
LOCUS       DAJTNV010000150         6375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNV010000150 DAJTNV010000000
VERSION     DAJTNV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="OO199_003683"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="OO199_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="OO199_003684"
                     /pseudo
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="OO199_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO199_003685"
     CDS             complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO199_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5537562.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO199_003686"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO199_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5537563.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="OO199_003687"
     CDS             complement(4517..5644)
                     /gene="efeO"
                     /locus_tag="OO199_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5537564.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5702..>6375)
                     /locus_tag="OO199_003688"
     CDS             complement(5702..>6375)
                     /locus_tag="OO199_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5537565.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1535 a         1680 c         1533 g         1627 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggaatgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggacgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtagacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttatatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca aacgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     5641 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     5701 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     5761 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
     5821 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
     5881 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     5941 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     6001 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
     6061 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     6121 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     6181 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
     6241 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     6301 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     6361 cgttaatgaa gatcc
//
LOCUS       DAJTNV010000151         6353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNV010000151 DAJTNV010000000
VERSION     DAJTNV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..369
                     /locus_tag="OO199_003689"
     CDS             <1..369
                     /locus_tag="OO199_003689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537566.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO199_003690"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO199_003690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5537567.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO199_003691"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO199_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5537568.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO199_003692"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO199_003692"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5537569.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO199_003693"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO199_003693"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5537570.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO199_003694"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO199_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5537571.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..>6353)
                     /locus_tag="OO199_003695"
     CDS             complement(5094..>6353)
                     /locus_tag="OO199_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HCR5537572.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1439 a         1836 c         1525 g         1553 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaggacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgcctggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agt
//
LOCUS       DAJTNV010000152         6327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNV010000152 DAJTNV010000000
VERSION     DAJTNV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..2202
                     /locus_tag="OO199_003696"
     CDS             <1..2202
                     /locus_tag="OO199_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5537573.1"
                     /translation="KANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEG
                     ECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAIN
                     KTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKN
                     NDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDEC
                     HRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDG
                     FLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVAR
                     TITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGK
                     AQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRID
                     ERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHD
                     DEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADG
                     KLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGK
                     DLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQE
                     IESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2269..3738
                     /locus_tag="OO199_003697"
     CDS             2269..3738
                     /locus_tag="OO199_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5537574.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3738..5399
                     /locus_tag="OO199_003698"
     CDS             3738..5399
                     /locus_tag="OO199_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5537575.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5620..5961
                     /gene="symE"
                     /locus_tag="OO199_003699"
     CDS             5620..5961
                     /gene="symE"
                     /locus_tag="OO199_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5537576.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6008..>6327)
                     /locus_tag="OO199_003700"
     CDS             complement(6008..>6327)
                     /locus_tag="OO199_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537577.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1859 a         1508 c         1457 g         1503 t
ORIGIN      
        1 aaagccaaca aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg
       61 ctggacgttc cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc
      121 gcagccgaag gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct
      181 gaactctggc aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt
      241 actcaggact attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca
      301 atcaacaaaa ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg
      361 accggaacag ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa
      421 aataaaaaac gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat
      481 aatgatttcc agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc
      541 gcttatgaaa ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa
      601 gcgtttaaac aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc
      661 ggcagcgcat ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc
      721 caaattggct taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc
      781 ggcgatccgg tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct
      841 tataaagttg tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg
      901 caaactgact taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat
      961 cgcacgatgg tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc
     1021 aagcgcacca atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa
     1081 cgtatgcgcc gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac
     1141 gtcatgaaaa tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac
     1201 ccgaaaaaac cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat
     1261 gctaaaacct gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag
     1321 caaattatcg gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc
     1381 ctcgacttta aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa
     1441 aaagtcatgg ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa
     1501 ctcgaagaaa tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg
     1561 ccaccatacc aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag
     1621 aaaatccgca agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag
     1681 tattacgacg ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca
     1741 ctgctcaaag aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc
     1801 aaagaagcga tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa
     1861 gaagtcggta aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg
     1921 ccgttaaccc gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac
     1981 tctgaagcag cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta
     2041 caggagatcg aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta
     2101 ccggagatta ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag
     2161 ctggaaaacg aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct
     2221 tctgcaacca tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc
     2281 tcagtaatca aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg
     2341 cagcgtctcg gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag
     2401 gcgctggaac tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc
     2461 agttgggccg ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat
     2521 gatctgtttc cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac
     2581 gtggtgaagc aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc
     2641 caggtgatca acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt
     2701 ggtgatattt acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc
     2761 tatactccac gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa
     2821 tccattatgg acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta
     2881 aagaacaaat acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt
     2941 gttgagaaaa aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt
     3001 gaagtaccgg tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat
     3061 gagcaactgg atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt
     3121 gagaagaact ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg
     3181 attgtggaag tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta
     3241 tttggcgaag gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat
     3301 accatcgtgc gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg
     3361 ttgttcttta ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg
     3421 gcgggcgtga aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag
     3481 atcgactggt ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg
     3541 tggaaagtca gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca
     3601 catcaggcgg aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag
     3661 caggcggaga tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct
     3721 ggcaaggagg ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga
     3781 cctcggcgtt gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt
     3841 atggtattaa gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc
     3901 cgcaggatcc gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag
     3961 cagagctggt gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg
     4021 aagagaagcc gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt
     4081 gctcaatttc tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat
     4141 atcctgttat cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt
     4201 taataaagtt aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta
     4261 ttgattttga ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt
     4321 gtgagcgttt ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga
     4381 gacattttaa agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc
     4441 gaatagttga aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc
     4501 tgaccagtct ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca
     4561 gccaaaacac cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc
     4621 tatttaccac cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa
     4681 tgggcaaact tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta
     4741 ttgcgcagga aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc
     4801 cgccaattag cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta
     4861 ttggtaatat agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat
     4921 atcaagtagc acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag
     4981 tgaaagaatc aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt
     5041 ctaaaatccg cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg
     5101 ctgatatgta tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt
     5161 ccattccatt tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta
     5221 attctgacag tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa
     5281 tatttgataa gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga
     5341 tttatcataa aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat
     5401 tccctcgccc tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa
     5461 atttatattt cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg
     5521 tatttactga tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc
     5581 accgctaatc acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt
     5641 gcacaaccgt tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat
     5701 gcgagtcgtt acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg
     5761 gaagccgccg gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt
     5821 gtcctcaccg cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag
     5881 gtgtgtaagc tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca
     5941 ggtaaacaga aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt
     6001 cgccgcgtta taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca
     6061 taatgtaagg aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc
     6121 gcccaacatt tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca
     6181 tattaagcga caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg
     6241 ctttaaacgc aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa
     6301 cttcatcgcg aagatgatcg aataaca
//
LOCUS       DAJTNV010000153         6321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNV010000153 DAJTNV010000000
VERSION     DAJTNV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            5..436
                     /locus_tag="OO199_003701"
     CDS             5..436
                     /locus_tag="OO199_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5537578.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO199_003702"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO199_003702"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5537579.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO199_003703"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO199_003703"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5537580.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO199_003704"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO199_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5537581.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO199_003705"
     CDS             complement(4438..5145)
                     /locus_tag="OO199_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5537582.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO199_003706"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO199_003706"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5537583.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO199_003707"
     CDS             complement(6003..6206)
                     /locus_tag="OO199_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5537584.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1402 a         1808 c         1647 g         1464 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga g
//
LOCUS       DAJTNV010000154         6268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNV010000154 DAJTNV010000000
VERSION     DAJTNV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..666)
                     /locus_tag="OO199_003708"
     CDS             complement(<1..666)
                     /locus_tag="OO199_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR5537585.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="OO199_003709"
     CDS             complement(656..2413)
                     /locus_tag="OO199_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5537586.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="OO199_003710"
     CDS             complement(2429..3313)
                     /locus_tag="OO199_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5537587.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="OO199_003711"
     CDS             complement(3324..3929)
                     /locus_tag="OO199_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5537588.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6268)
                     /locus_tag="OO199_003712"
     CDS             complement(4100..>6268)
                     /locus_tag="OO199_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5537589.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1835 a         1568 c         1316 g         1549 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgttt
//
LOCUS       DAJTNV010000155         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNV010000155 DAJTNV010000000
VERSION     DAJTNV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO199_003713"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO199_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5537590.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO199_003714"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO199_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5537591.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO199_003715"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO199_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO199_003716"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO199_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1456 c         1477 g         1665 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgat
//
LOCUS       DAJTNV010000156         6213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNV010000156 DAJTNV010000000
VERSION     DAJTNV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..378
                     /locus_tag="OO199_003717"
     CDS             <1..378
                     /locus_tag="OO199_003717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537592.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO199_003718"
     CDS             complement(454..918)
                     /locus_tag="OO199_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5537593.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO199_003719"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO199_003719"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5537594.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO199_003720"
     CDS             complement(1787..2476)
                     /locus_tag="OO199_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5537595.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO199_003721"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO199_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5537596.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO199_003722"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO199_003722"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5537597.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO199_003723"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO199_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5537598.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1576 a         1688 c         1547 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg aca
//
LOCUS       DAJTNV010000157         6018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNV010000157 DAJTNV010000000
VERSION     DAJTNV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.157"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO199_003724"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO199_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5537599.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO199_003725"
     CDS             1207..1869
                     /locus_tag="OO199_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5537600.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO199_003726"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO199_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5537601.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO199_003727"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO199_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO199_003728"
     CDS             2795..3100
                     /locus_tag="OO199_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5537602.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO199_003729"
     CDS             3103..3507
                     /locus_tag="OO199_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5537603.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO199_003730"
     CDS             3497..3796
                     /locus_tag="OO199_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5537604.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO199_003731"
     CDS             3982..4278
                     /locus_tag="OO199_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5537605.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO199_003732"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO199_003732"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5537606.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO199_003733"
     CDS             5627..5995
                     /locus_tag="OO199_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5537607.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1522 a         1468 c         1517 g         1511 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaaaca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggt
//
LOCUS       DAJTNV010000158         5995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNV010000158 DAJTNV010000000
VERSION     DAJTNV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..263)
                     /locus_tag="OO199_003734"
     CDS             complement(<1..263)
                     /locus_tag="OO199_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5537608.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            484..912
                     /gene="uspG"
                     /locus_tag="OO199_003735"
     CDS             484..912
                     /gene="uspG"
                     /locus_tag="OO199_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5537609.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO199_003736"
     CDS             complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO199_003736"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5537610.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO199_003737"
     CDS             complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO199_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5537611.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3778..4524
                     /gene="dsbG"
                     /locus_tag="OO199_003738"
     CDS             3778..4524
                     /gene="dsbG"
                     /locus_tag="OO199_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5537612.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4734..5636
                     /locus_tag="OO199_003739"
     CDS             4734..5636
                     /locus_tag="OO199_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5537613.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5783..5986
                     /locus_tag="OO199_003740"
                     /pseudo
     CDS             5783..5986
                     /locus_tag="OO199_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1610 a         1444 c         1350 g         1591 t
ORIGIN      
        1 atgacgaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt
       61 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
      121 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata
      181 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
      241 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
      301 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag
      361 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
      421 atcagggata aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg
      481 gttatgtata aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
      541 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
      601 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
      661 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
      721 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
      781 ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg
      841 acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg
      901 gtcgttcgct aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct
      961 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
     1021 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
     1081 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
     1141 cgctgcgaac acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat
     1201 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
     1261 aaaaataccg gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag
     1321 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
     1381 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
     1441 aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
     1501 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcggtc cgtcgcagtg
     1561 cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg
     1621 ccattttgca cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
     1681 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
     1741 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg
     1801 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
     1861 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
     1921 cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
     1981 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
     2041 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
     2101 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
     2161 gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
     2221 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
     2281 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
     2341 ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat
     2401 caggaaagac ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga
     2461 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
     2521 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
     2581 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
     2641 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
     2701 cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg
     2761 aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta
     2821 tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag
     2881 ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact
     2941 gagctgcttt gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa
     3001 cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac
     3061 cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca
     3121 tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt
     3181 gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt
     3241 cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga
     3301 agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga
     3361 atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg
     3421 tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta
     3481 tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc
     3541 cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc
     3601 tttggtatct tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga
     3661 catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg
     3721 acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg
     3781 ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct
     3841 tccccagtaa aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc
     3901 ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact
     3961 cccgatggta agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt
     4021 aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg
     4081 gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc
     4141 tccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat
     4201 tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg
     4261 acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc
     4321 tctggttgca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg
     4381 ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat
     4441 tacatgagta aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt
     4501 aatatcatta tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct
     4561 tttcaaccat cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt
     4621 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa
     4681 aacattgact aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca
     4741 atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc
     4801 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
     4861 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
     4921 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
     4981 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct
     5041 acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
     5101 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
     5161 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg
     5221 tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc
     5281 gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag
     5341 ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca
     5401 aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt
     5461 tactgggaat agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag
     5521 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
     5581 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
     5641 taactataga cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa
     5701 acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg ttcaccctgt
     5761 aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct
     5821 ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc
     5881 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
     5941 aatgggcaaa aaaatctgcg ttttgtttat caactgggag gcgcaatgaa tcgcc
//
LOCUS       DAJTNV010000159         5890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNV010000159 DAJTNV010000000
VERSION     DAJTNV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1196
                     /gene="narU"
                     /locus_tag="OO199_003741"
     CDS             <1..1196
                     /gene="narU"
                     /locus_tag="OO199_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5537614.1"
                     /translation="NFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWPVFSTAI
                     LIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGI
                     NGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIAT
                     IAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQF
                     PDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTG
                     SGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTE
                     TAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRR
                     KFSQK"
     gene            1278..5018
                     /gene="narZ"
                     /locus_tag="OO199_003742"
                     /pseudo
     CDS             1278..5018
                     /gene="narZ"
                     /locus_tag="OO199_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5015..>5890
                     /gene="narH"
                     /locus_tag="OO199_003743"
     CDS             5015..>5890
                     /gene="narH"
                     /locus_tag="OO199_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5537615.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1368 a         1586 c         1587 g         1349 t
ORIGIN      
        1 ttaattttac taccgatcaa ctctttttat taaccgcatt accctccgtt tctggcgcat
       61 tattgcgtgt tccctactcc tttatggtgc ctatattcgg tggacgccga tggccggttt
      121 ttagtactgc aatcctgatt attccttgcg tctggctcgg aattgccgtg caaaatccga
      181 atactccttt tgggatattt atcgttatcg ctttgctatg cggttttgca ggtgcaaact
      241 ttgcttcgag catgggcaat atcagtttct tctttccaaa agccaaacaa gggagcgctc
      301 ttgggattaa tggcggatta ggaaacttag gtgtaagtgt aatgcagctg gttgcaccgc
      361 tggtcatttt tgtacctgta tttgcctttc tcggcgtcaa tggcgtaccg caggccgacg
      421 gttcggtgat gtcgctggcg aatgccgcat ggatttgggt accgctactg gcgattgcca
      481 cgatcgccgc ctggtcaggg atgaatgata tcgccagttc acgcgcctca attgccgacc
      541 agctccctgt cttacaacgc ctgcatctct ggctgctgag cctgctttac cttgccacct
      601 tcggttcgtt tatcggtttt tctgcgggtt ttgccatgct ggcaaaaacc cagttcccgg
      661 atgtgaatat tctgcgcctg gcgttctttg gcccatttat tggtgccatc gcgcggtcgg
      721 ttggtggtgc tatttccgat aaattcggcg gcgtgcgggt gacgttgatc aactttattt
      781 ttatggcgat tttcagtgcc ctgctgttcc ttaccttacc gggcacaggc tccggtaatt
      841 tcatcgcctt ttacgccgta tttatggggc tgtttctgac cgcgggtctg ggaagtggtt
      901 ctactttcca gatgatcgcc gtcatctttc gccagataac catttatcgg gtgaagatga
      961 aaggcggtag tgatgagcaa gctcagagag aagccgtcac cgaaacggcg gcaacgctgg
     1021 gctttatctc agccattggc gcagtgggcg gcttttttat tccgcaggca tttggcatgt
     1081 cgctcaatat gaccggctct ccggtcggcg cgatgaaagt gtttttaatc ttctacatcg
     1141 tttgtgtgct gctgacctgg ctggtttatg gtcggcggaa gttcagccaa aaataagaga
     1201 tcgatgcctg atgtacaacc atgtcgcatc cgacaaggca tcagatccca attcattcct
     1261 ggagcaggag tcatgtcatg agtaaacttt tggatcgctt tcgctacttc aaacaaaagg
     1321 gcgaaacctt tgccgatggt cacggacagg tgatgcatag caaccgcgac tgggaggaca
     1381 gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc cacccacggc gttaactgta
     1441 caggctcctg tagctggaaa atctacgtta aaaatggtct ggtgacctgg gaaatccaac
     1501 agaccgacta cccgcgcact cgccctgacc tgcccaatca tgaacctcgc ggctgcccgc
     1561 gtggcgcaag ttactcctgg tatctttaca gcgctaaccg cctgaaatac ccgctcattc
     1621 gtaaacgact gattgaactg tggcgcgaag ccctcaaaca acacagcgat ccggtactgg
     1681 cgtgggcatc gattatgaac gatccgcaaa agtgcctgag ctacaaacaa gtgcgtgggc
     1741 gcggcgggtt tatccgctcc aactggcagg aactaaacca gctgattgcc gccgctaacg
     1801 tctggaccat caaaacctac ggcccggatc gcgttgccgg tttctcgccg atcccggcga
     1861 tgtcgatggt ttcttacgcc gccggaacgc gttatctgtc gctgcttggc ggcacctgtt
     1921 aaagtttcta cgactggtat tgcgacctgc cgcccgcctc gccgatgacc tggggcgagc
     1981 aaaccgacgt accggaatct gccgactggt ataactccag ctacatcatc gcttgggggt
     2041 ctaacgtacc gcagacacgt acgccggacg cccacttctt taccgaagta cgctacaaag
     2101 gcactaaaac catcgccatt acccctgact actctgaagt ggccaaattg tgcgaccagt
     2161 ggctggcacc gaaacaaggc actgatagcg ccctggcgat ggcaatgggg catgtgattt
     2221 taaaagagtt tcatctcgat aatcccagcg actactttat caactactgc cgccgctaca
     2281 gcgacatgcc gatgctggta atgctggagc ctcgcgacga tggtagctac gttcccgggc
     2341 gcatgatccg cgcatctgac ctggtggatg gactgggcga aagcaacaat ccgcagtgga
     2401 aaaccgtagc agttaatacc gcaggtgaat tggtagtgcc gaacggttcg attggtttcc
     2461 gctggggaga aaaaggcaaa tggaatctgg aatccattgc cgccggtacg gaaaccgaat
     2521 tgtcgttaac cctgctcggt caacatgacg ctgttgcagg cgtggccttc ccctactttg
     2581 gcggcattga aaatccgcat tttcgcagcg taaaacacaa tccggtgctg gtgcgccaat
     2641 tgcccgttaa aaacctgacg ttagtcgatg gcaacacctg tccggtggtc agcgtttatg
     2701 atttggtact ggcgaattac ggcctcgatc gcgggctgga agatgaaaac agcgcgaaag
     2761 attacgctga aatcaaaccg tacaccccag cctggggtga gcaaattacc ggcgtaccgc
     2821 gccagtatat tgaaaccatc gcccgcgaat ttgccgatac cgcccataaa acgcatgggc
     2881 gctcaatgat tatcctcggc gcaggtgtta accactggta tcacatggac atgaactacc
     2941 gcgggatgat caatatgctg atcttctgcg gttgtgtcgg gcaaagcggt ggcggctggg
     3001 cacactatgt cggccaggaa aaactgcgcc cacaaaccgg ctggttgcca ctggcctttg
     3061 cgctcgactg gaaccgacca ccgcgccaga tgaacagcac ctcgtttttc tacaatcatt
     3121 ccagccaatg gcgctatgaa aaagtctctg ctcaggagtt actttcaccg ctcgccgatg
     3181 ccagtaagta cagcggtcat ctgattgatt tcaacgttcg cgccgaacgt atgggctggc
     3241 taccttctgc gccgcagctg gggcgtaacc cgctcgggat taaagctgaa gccgacaagg
     3301 ccggattatc ccccacagaa tttaccgccc aggcgctgaa atcgggcgat ttacgtatgg
     3361 cctgcgaaca accagatagc ggcagcaatc atccgcgtaa tttgtttgtc tggcgctcta
     3421 acctgcttgg ctcctccggc aaaggccacg agtatatgca gaagtatctg ctggggaccg
     3481 aaagcgggat tcagggcgag gaactcggtg ccagcgacgg gatcaaaccg gaagaagtcg
     3541 agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct ggtgacgctc gacttccgca
     3601 tgtccagtac ctgcctgttc tccgatatcg ttctgcccac cgccacctgg tatgaaaaag
     3661 acgatatgaa cacctcggat atgcatccat ttattcatcc gctttctgcg gcggtcgatc
     3721 cggcctggga atcacgcagc gactgggaaa tctacaaagg tattgccaaa gcattttcgc
     3781 aagtgtgcgt gggccatctt ggcaaagaaa ccgacgtggt attacaacca ctgctgcatg
     3841 actctccggc agagctctca cagccgtgtg aagtgctcga ctggcgcaaa ggcgaatgcg
     3901 atctgatccc gggtaaaacc gcgccgaata ttgtggcggt ggagcgcgac taccctgcta
     3961 cgtatgaacg ctttacctcg ctcgggccat tgatggacaa acttggcaat ggcggtaaag
     4021 ggatttcgtg gaatacccag gatgaaatcg atttcctcgg caaactcaat tacaccaagc
     4081 gtgatggccc ggcacagggg cgtccgctga ttgacaccgc cattgacgct tcagaagtga
     4141 ttctggcact ggcaccggaa accaacggtc atgttgcagt caaagcgtgg caggcgctgg
     4201 gcgagatcac cgggcgcgaa catacccatc tggcgctgca caaagaggac gagaagattc
     4261 gctttcgcga tattcaggcg cagccgcgta aaattatctc cagccccaca tggtccggtc
     4321 tggaaagcga tcacgtatcc tataacgcgg gatacaccaa cgttcatgag ttaattccgt
     4381 ggcgcacgct gtcgggacgc cagcagctct atcaggatca tccgtggatg cgtgcttttg
     4441 gtgaaagcct ggtggcttat cgcccgccta tcgacacccg tagcgtcagt gagatgcgcc
     4501 agataccgcc aaacggcttc caggaaaaag cacttaactt cctgacgccg caccagaaat
     4561 ggggcattca ctcaacctac agtgaaaacc tgctaatgct gacgctctct cgcggtggac
     4621 cgattgtctg gatcagcgaa acagatgccc gagaactgac cattgtcgat aacgactggg
     4681 tggaagtatt taacgccaac ggcgcgctga ctgcccgcgc ggtggtcagc caacgtgtac
     4741 cgccgggcat gaccatgatg tatcacgccc aggaacgcat tatgaatatt cctggttcgg
     4801 aagtaactgg catgcgcggc ggcattcata actcggtcac ccgcgtttgc ccgaaaccga
     4861 cgcatatgat tggcggttac gcgcagctgg cctggggctt taactactac ggcaccgtcg
     4921 gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa gaacgttaac tggctggatg
     4981 atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa atacgttcgc aagtcggcat
     5041 ggtgcttaac ctcgataaat gtatcggctg ccatacctgt tcggtgacct gtaacaacgt
     5101 ctggaccgga cgcgaaggca tggagtacgc atggtttaac aacgtcgaaa ccaaaccggg
     5161 cattggttat ccgaaaaact gggaagatca ggaagagtgg caaggcggct gggtccgcga
     5221 tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg ggcgtaataa ccaaaatctt
     5281 cgccaacccg gtggtgccgc agcagattga tgattactac gaacctttca ccttcgacta
     5341 cgaacatttg catagcacac cggaaggcaa acatatccct actgctcgcc cgcgttcact
     5401 gattgacggc aaacggatgg acaaagtgat ctggggccca aactgggaag aactgctggg
     5461 cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag gccatgcaaa aggagatgta
     5521 cgggcagttt gaaaacacct tcatgatgta cctgccgcgc ctgtgcgaac actgcctcaa
     5581 tcccagctgc gtggcgacct gcccaagcgg cgctatctac aaacgcgaag aagacggcat
     5641 tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta
     5701 caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa aaatgtatct tctgttatcc
     5761 gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc tgcgtgggtc gcatccggta
     5821 tctgggcgtg ctgctttacg acgccgaccg catcgaggaa gcgtcgagca ccgagcgcga
     5881 agttgacctc
//
LOCUS       DAJTNV010000160         5839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNV010000160 DAJTNV010000000
VERSION     DAJTNV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            430..1338
                     /locus_tag="OO199_003744"
     CDS             430..1338
                     /locus_tag="OO199_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5537616.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO199_003745"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO199_003745"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5537617.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO199_003746"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO199_003746"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5537618.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO199_003747"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO199_003747"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5537619.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>5839)
                     /locus_tag="OO199_003748"
     CDS             complement(5673..>5839)
                     /locus_tag="OO199_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537620.1"
                     /translation="SYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCSGQAFPADN
                     NGYAPPPCRK"
BASE COUNT         1433 a         1523 c         1520 g         1363 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaat
//
LOCUS       DAJTNV010000161         5807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTNV010000161 DAJTNV010000000
VERSION     DAJTNV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO199_003749"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO199_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5537621.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="OO199_003750"
     CDS             580..1029
                     /locus_tag="OO199_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5537622.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /locus_tag="OO199_003751"
     CDS             complement(1030..1263)
                     /locus_tag="OO199_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5537623.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO199_003752"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO199_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5537624.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="OO199_003753"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="OO199_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5537625.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2214..3023)
                     /locus_tag="OO199_003754"
     CDS             complement(2214..3023)
                     /locus_tag="OO199_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5537626.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO199_003755"
     CDS             complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO199_003755"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5537627.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4679..5473)
                     /locus_tag="OO199_003756"
     CDS             complement(4679..5473)
                     /locus_tag="OO199_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5537628.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5463..>5807)
                     /locus_tag="OO199_003757"
     CDS             complement(5463..>5807)
                     /locus_tag="OO199_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5537629.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1524 a         1473 c         1286 g         1524 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttatatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggca acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagagac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacact gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt
     2281 gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt attattatcc
     2341 ggaagatctg agtagttata attccaccat tttattctca acagttcatc aataacattt
     2401 gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
     2461 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
     2521 cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
     2581 ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca
     2641 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc
     2701 attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa
     2761 tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa
     2821 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
     2881 aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
     2941 caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg
     3001 aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt agtttaataa
     3061 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
     3121 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
     3181 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
     3241 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     3301 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     3361 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
     3421 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
     3481 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
     3541 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     3601 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
     3661 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     3721 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
     3781 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
     3841 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     3901 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
     3961 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
     4021 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     4081 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     4141 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     4201 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     4261 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     4321 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     4381 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     4441 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     4501 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     4561 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     4621 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     4681 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     4741 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     4801 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     4861 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     4921 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     4981 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     5041 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     5101 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     5161 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     5221 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     5281 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     5341 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     5401 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     5461 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ctaggtacag tgcgatgaga
     5521 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     5581 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     5641 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     5701 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     5761 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggat
//
LOCUS       DAJTNV010000162         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTNV010000162 DAJTNV010000000
VERSION     DAJTNV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..219)
                     /locus_tag="OO199_003758"
     CDS             complement(<1..219)
                     /locus_tag="OO199_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5537630.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="OO199_003759"
     CDS             complement(216..1124)
                     /locus_tag="OO199_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5537631.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="OO199_003760"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="OO199_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO199_003761"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OO199_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO199_003762"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OO199_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5537632.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="OO199_003763"
     CDS             5533..>5702
                     /locus_tag="OO199_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537633.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1338 a         1480 c         1471 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtaggccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAJTNV010000163         5684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNV010000163 DAJTNV010000000
VERSION     DAJTNV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            873..3158
                     /gene="nrdA"
                     /locus_tag="OO199_003764"
     CDS             873..3158
                     /gene="nrdA"
                     /locus_tag="OO199_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5537634.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3392..4522
                     /gene="nrdB"
                     /locus_tag="OO199_003765"
     CDS             3392..4522
                     /gene="nrdB"
                     /locus_tag="OO199_003765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5537635.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4522..4776
                     /gene="yfaE"
                     /locus_tag="OO199_003766"
     CDS             4522..4776
                     /gene="yfaE"
                     /locus_tag="OO199_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5537636.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4830..5480)
                     /gene="inaA"
                     /locus_tag="OO199_003767"
     CDS             complement(4830..5480)
                     /gene="inaA"
                     /locus_tag="OO199_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5537637.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1488 c         1350 g         1390 t
ORIGIN      
        1 gatactccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
       61 gatatcatat ccctggcata aatcaatggc cgccgcgaca ccgttagcag agaaaagaga
      121 tgcaatcatt gacgggagta gagataaata ctccttgcat agaaagataa tccgcatatt
      181 aatctgacca tcattttata taagacggca tatttaaacg cattatacaa tatgggttat
      241 ctttcttttg taaattgtcc aacccgatgc aatggctatt ttgttattat ttgatgaata
      301 atatcagtgc gtcataattc aagttaataa ccttaaggga taccagttat atttaaacta
      361 aattaaagta atgaataatt ttcttataat ataaggtaaa ttaacaaaat ggcttcgcat
      421 ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
      481 tatattgacc acaactgata catcagatta tgtgatgact cgtgcttaga tcaatttttg
      541 caatcattag caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
      601 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
      661 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
      721 agttatgcac ttgcaagagg gtcattttca cactatcttg cagtgaatcc caaacatacc
      781 ccctatatat agtgttctaa gcagcttccc ttactacagg tagtctgcat gaaactattg
      841 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
      901 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
      961 cagaaggact gcataacgtt tcgatttccc aggtcgagct gcgctcccac attcagtttt
     1021 atgacggtat caagacctct gacatccacg aaactattat caaggctgcc gcagacctga
     1081 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcgatc ttccacctgc
     1141 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
     1201 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
     1261 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
     1321 agctggaagg caaatatcta gtacagaacc gcgtgaccgg cgaaatctac gaaagcgccc
     1381 agttccttta tattcttgtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
     1441 tgcaatacgt gaagcgtttt tatgacgcgg tttccacatt taaaatttcg ctgccgacgc
     1501 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
     1561 gcggtgacag cctggattcc atcaacgcca cctccagcgc gattgtgaaa tatgtttccc
     1621 agcgtgccgg gatcggcatc aacgccggac gtattcgtgc gctgggcagc ccgattcgcg
     1681 gtggcgaagc gttccatacc ggctgtatcc cgttctacaa acatttccag acagcggtga
     1741 aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac gctgttctac ccgatgtggc
     1801 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggggttgaa ggcaaccgcg
     1861 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgc ctgctgaaag
     1921 gtgaagatat caccctgttc agcccgtccg acgtaccggg gctgtacgac gcgttcttcg
     1981 ccgatcagga agagtttgaa cgcctgtata ccaaatatga gaaagacgac agcatccgca
     2041 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
     2101 gtcgtatcta tattcagaac gttgaccact gtaataccca tagtccgttt gatccgacca
     2161 tcgcgccagt tcgccagtct aacctgtgcc tggaaattgc cctgccgacc aaaccgctga
     2221 acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
     2281 gcgcaattaa tagcctggat gaactggaag agctggcgat tctggcggtt cgtgcacttg
     2341 acgcgctact ggattatcag gattacccga tcccggccgc caaacgtgga gcgatggggc
     2401 gtcgtacgct gggtattggt gtgattaact tcgcttacta tctggcgaag cacggtaaac
     2461 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcg attcagtatt
     2521 acctgctgaa agcgtctaat gagctggcta aagagcaagg cgcgtgcccg tggtttaacg
     2581 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatgcca
     2641 tcgctaatga gccgctgcat tacgactggg aagcattgcg tgaatcaatc aaaacgcacg
     2701 gcctgcgtaa ctccacgctt tctgccctga tgccgtccga gacctcttcg cagatctcta
     2761 acgccactaa cggtattgaa ccgccgcgcg gttacgtcag catcaaagcg tcgaaagacg
     2821 gtattttgcg ccaggtggtg ccggactacg agcacctgca cgacgcctat gagctgctgt
     2881 gggaaatgcc gggtaacgat ggctacctgc aactggtagg tatcatgcag aaatttatcg
     2941 atcagtcgat ctctgctaac accaactacg atccgtcacg tttcccgtca ggaaaagtgc
     3001 cgatgcagca gttgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
     3061 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
     3121 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
     3181 taaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
     3241 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     3301 taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     3361 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     3421 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     3481 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     3541 tccggaagaa gttgacgtct cccgcgaccg tatagattac caggcgctgc cggagcacga
     3601 aaaacacatc tttatcagca acctgaaata tcagacgctg ctggattcca ttcagggtcg
     3661 tagcccgaac gtggcgctat tgccgcttat ttctattccg gaactggaaa cctgggtcga
     3721 aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     3781 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     3841 tgcggaaggg atctccagct attacgatga actgatcgaa atgaccagct actggcacct
     3901 tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta accgttagcc tgcgcgagct
     3961 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga tccgcttcta
     4021 cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag gtaacgccaa
     4081 gattattcgc ctgattgccc gcgatgaagc cctgcacctg accggtactc agcatatgct
     4141 gaatctgctg cgcagcggcg cggatgatcc ggagatggcg gaaattgccg aagaatgtaa
     4201 gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact gggcggatta
     4261 tctgttccgt gacggttcga tgattggtct gaataaagat attctctgcc agtacgttga
     4321 atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     4381 caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     4441 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcagaagtgg acaccgacga
     4501 tttgagtaac ttccagctct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     4561 ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgtggcggtt
     4621 gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cccgactggt cgcaggtcag
     4681 gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     4741 tgccgggcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc cagatacgac
     4801 gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     4861 tcccagtccg ctttagggat cggctccaga tatttttcca gctggcggaa gtcatgatta
     4921 atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa accagcttct
     4981 gccttacctt ctgttttcac atagatatgg cgaacatagc aacagccatg ctgacgatta
     5041 acgctatgca ttttcttaaa cgccagtgcc actgctttca acatggcttg ccgtacttcg
     5101 tcagaataag gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     5161 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     5221 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     5281 acaatcgttg gtcggccgaa cggataacgt actgaatgaa acagatgatg cgtcatgcgc
     5341 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     5401 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5461 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     5521 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccgc
     5581 tacaggtgtg acaccgcttt tgcccttcat ttgcagcgca tagattccac cagccactac
     5641 cactaatagt gcgcccagca gataaaatgt gaatttaatc agtc
//
LOCUS       DAJTNV010000164         5642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNV010000164 DAJTNV010000000
VERSION     DAJTNV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            263..1189
                     /gene="tauA"
                     /locus_tag="OO199_003768"
     CDS             263..1189
                     /gene="tauA"
                     /locus_tag="OO199_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5537638.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1202..1969
                     /gene="tauB"
                     /locus_tag="OO199_003769"
     CDS             1202..1969
                     /gene="tauB"
                     /locus_tag="OO199_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5537639.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1966..2793
                     /gene="tauC"
                     /locus_tag="OO199_003770"
     CDS             1966..2793
                     /gene="tauC"
                     /locus_tag="OO199_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5537640.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2790..3641
                     /gene="tauD"
                     /locus_tag="OO199_003771"
     CDS             2790..3641
                     /gene="tauD"
                     /locus_tag="OO199_003771"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5537641.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="OO199_003772"
     CDS             complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="OO199_003772"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5537642.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5245..5613
                     /locus_tag="OO199_003773"
                     /pseudo
     CDS             5245..5613
                     /locus_tag="OO199_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1325 a         1410 c         1529 g         1378 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 atataacaca cttcttgccg cactggcatt catcgctttt caggcacagg cggtgaacgt
      301 caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg gctcaggccg acaacacctt
      361 tgctaaagag agcggggcga gcgtggactg gcgtaagttt gacagcggag ccagcatcgt
      421 gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc ggttccagcc cattagcggt
      481 tgcagccagc caacaggtgc cgattgaagt cttcttgctg gcgtcaaaac tgggtaactc
      541 cgaagcgctg gtggtaaaga aaactatcag caaacccgaa gatttgattg gtaaacgcat
      601 tgccgtaccg tttatctcca ccacccacta cagcctgctg gcggcgctga aacactgggg
      661 gattaaacca gggctagtgg agattgtgaa cctgcagccg cccgcgatta tcgccgcatg
      721 gcagcggggt gatattgatg gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa
      781 agacggcaag gtgctgaccg attctgaaca ggtcgggcag tggggtgcgc caacgctgga
      841 cgtctgggtg gtgcgcaaag attttgccga gaaacatcct gaggtcgtga aagcgttcgc
      901 caaaagcgcc atcgatgcac agcaaccgta cattgctaac cctgacgcgt ggctgaaaca
      961 gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg cctgaaggtg acgttccggg
     1021 gctggtgaag ggaaatacct atctgacgcc gcagcaacaa acggcagaac tgaccggacc
     1081 ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa gagcagggca aggtcccggc
     1141 tgtagcgaat gattacagcc agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca
     1201 gatgctgcaa atctctcatc tctatgccga ttatggcggc aaaccggcac tggaagatat
     1261 caacctgacg ctggaaagcg gcgagctact ggtggtgctg gggccgtccg gctgcggtaa
     1321 aaccaccctg ctgaatctga ttgccggttt tgtgccttat cagcatggca gcattcaact
     1381 ggcgggtaag cgtattgagg gaccgggagc agagcgtggc gtagtttttc agaatgaagg
     1441 gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat
     1501 agagaaaatg cagcgactgg aaatcgcgca ccagatgctg aaaaaagtgg ggctggaagg
     1561 cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa cgtcagcggg tggggattgc
     1621 ccgcgcgctg gcggcgaatc cccagctgtt attactcgac gaaccgtttg gtgcgctgga
     1681 cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa ctctggcagg agacgggcaa
     1741 gcaggtgctg ttgattaccc acgatataga agaagcggtg tttatggcga ctgaactggt
     1801 tctgctttca tccggccctg gccgtgtgct ggagcggctg cggctcaact ttgctcgccg
     1861 ctttgttgcg ggagagtcga gccgcagcat caagtccgat ccacaattca tcgccatgcg
     1921 cgaatatgtt ttaagccgcg tatttgagca acgggaggcg ttctcatgag tgtgctcatt
     1981 aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc
     2041 ttaagcattg gcacgttagc ggttttactc actgtatggt ggacggtggc ggcgctgcaa
     2101 ctgattagcc cgctgttttt gccgccgccg caacaagtac tggcaaaact gctcaccatt
     2161 gccggaccgc agggatttat ggacgccacg ctgtggcagc atctggcagc cagcctgacg
     2221 cgcattgtgc tggcgctact cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg
     2281 gggctaagcc cgacggtgcg cggcattctg gatccggtaa tcgaacttta tcgtccggtg
     2341 ccaccgctgg cttatttgcc gctgatggtg atctggtttg gtattggtga aacctcgaag
     2401 atcttactga tctatttagc gatttttgcg ccggtggcga tgtcggcgct ggcaggggtg
     2461 aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag
     2521 gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt
     2581 ggtctgggtg tgggctggtc tacgctggta gcggcggagc tgattgccgc gacacgcggt
     2641 ttaggattta tggttcagtc agcgggtgaa tttctcgcga ctgacgtggt actggcgggg
     2701 atcgcggtga ttgcgattat cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc
     2761 cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg tctgagcatt accccgctgg
     2821 ggccgtatat cggcgcacaa atttcgggtg ccgacctgac gcgcccgtta agcgataatc
     2881 agtttgaaca gctttaccat gcggtgctgc gccatcaggt ggtatttctg cgcgatcaag
     2941 ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt tggcgaactg catattcacc
     3001 ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt gctggatacc cataacgata
     3061 atccgccgga taacgacaac tggcataccg atgtgacatt tattgaaacg ccacccgcag
     3121 gggcgattct ggcagctaaa gagttacctt cgaccggcgg tgatacgctc tggaccagcg
     3181 gtattgcggc ctatgaggcg ctctctgttc ccttccgcca gctgctgagc tggctgcgtg
     3241 cggagcatga tttccgtaaa tcattcccgg aatacaaata ccgcaaaacc gaggaggaac
     3301 atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa
     3361 cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga aggctttact acgcgaattg
     3421 tcgatgtcag cgagaaagag agcgaagcct tgttaggctt tttattcgcc catatcacta
     3481 aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga tattgcgatt tgggataacc
     3541 gcgtgactca gcactatgcc aatgccgatt acctgccaca gcggcggatt atgcatcggg
     3601 cgacgatcct tggggataaa ccgttttatc gggcggggtg atacgagtgc ggacggttcc
     3661 ctcgccccct tggggagagg gttagggtga gggggcgttc actgtacttt caacaggtta
     3721 actccccctt tctgagagga aaaaaattaa cgcagaatct tcttctcagc caaatccagc
     3781 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc
     3841 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3901 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3961 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     4021 agtgactcac gaatcgcctc gcgacggttc attgggttca tctgatagct tttgcggtcg
     4081 ccttttaatg cgcttcctgc agcttcacgg aacgggccat aaaaggaaga ggcgaacttg
     4141 gtcgaatacg acataatcgc cgtatcttta aagcctgcag cgtccagcgc ctgacgaatc
     4201 gcctgtacct ggccgtccat cgcggcggaa ggggcgatga agtctgcgcc tgcagcagct
     4261 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4321 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4381 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4441 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccaaaagt cattacggaa
     4501 cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4561 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4621 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4681 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
     4741 gaattaaggt gcacagtata cctgaagcgg ggcaggagtg ttttacgaaa gttgtcgcga
     4801 tgttgacaag aagagaatga aagagagacc aggacatctt tgtaatagaa atagcaataa
     4861 tttcatattc actgaaaata tttttaatct ttatttatag ctcttggtta ttattttttg
     4921 gcgtttggtt gtgctgctat aaattgaata attcaaactt tgttgcatat tgataagata
     4981 tttatctggt ttgtgttttt gatatttata acgcgtacgc atcaaaaata acattatctg
     5041 caacatactg aaatatcgtc cttttatcaa tttgatgcgt gtttttctcc ataaattata
     5101 tatgtccaca ttcggactta ggggaaagaa taattgaacc attcgtctgt aacgcagcat
     5161 aatcgttagc gcgaaacata atatgttttc tatgcattga taattgatgg atcaacttat
     5221 tacgtccctg aggagggatg acaaatgcac tcctggaaaa agaaacttgt agtatcacaa
     5281 ttagcattgg cttgcactct ggctatcacc tctcaggcta atgcagcgac caacgatatt
     5341 tctggtcaaa cttacaatac tttccatcac tacaacgacg ccacctatgc tgatgacgtt
     5401 tactatgatg gttatgtagg ctggaacaac tatgccgctg atagctatta caacggcgat
     5461 atctacccgg tcattaataa cgctaccgtt aacggcgtga tttctaccta ctatctggac
     5521 gacggtattt ctaccaatac caacgccaat agtctgacaa tcaaaaacag cactattcac
     5581 ggtatgattt attccgagtg catgacaact gattagactg gccccctgaa tctccagaca
     5641 ac
//
LOCUS       DAJTNV010000165         5612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNV010000165 DAJTNV010000000
VERSION     DAJTNV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            382..1410
                     /gene="torT"
                     /locus_tag="OO199_003774"
     CDS             382..1410
                     /gene="torT"
                     /locus_tag="OO199_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5537643.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1383..2075)
                     /gene="torR"
                     /locus_tag="OO199_003775"
     CDS             complement(1383..2075)
                     /gene="torR"
                     /locus_tag="OO199_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5537644.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2162..3334
                     /gene="torC"
                     /locus_tag="OO199_003776"
     CDS             2162..3334
                     /gene="torC"
                     /locus_tag="OO199_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5537645.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3334..>5612
                     /gene="torA"
                     /locus_tag="OO199_003777"
     CDS             3334..>5612
                     /gene="torA"
                     /locus_tag="OO199_003777"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR5537646.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAP"
BASE COUNT         1365 a         1403 c         1537 g         1307 t
ORIGIN      
        1 tccatttcgg tcccagttag gacgctttct catcaagcca gtccgcccac cactgcatca
       61 tctccctacg cttatcgaga tactgagcat ggttgtaaat tcaattaacc cagtgcgttg
      121 acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct aatgctctgt tacaggtcac
      181 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg tcgcgttttt
      241 cagtctatta tttaatcatt aagtaatcta atgctatgat ttttaaatta tttatgtgat
      301 tttgtcatcc tttaggtgaa aaaggttgag tcgcaaagtg gaatgcatct agcataaagc
      361 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
      421 ttgtcggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
      481 caagcctcta tgccgcttaa agccaaacgc gtatggaaac tgtgcgcgct ttatcccagc
      541 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
      601 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
      661 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
      721 tcatttcccg acctgcaaaa gcaggtagca aatctgccgg tgatcgaact ggtaaatgct
      781 cttgatgatc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
      841 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gctgatgccc
      901 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
      961 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgc
     1021 aacctgttgc aggagatgct ggaacgccac ccagaaatcg acgtcgttgc cggaacggcc
     1081 attgcggcag aggcggcaat gggtgaaggg cgtaacctga aaacgccgct taccgtggtg
     1141 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
     1201 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
     1261 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
     1321 gccgaccgag aacatatccg ccgctcgctg tcaccggggg gatttcgtcc ggtctatttt
     1381 tatcagcaca catcagcggc taagaaataa ccttcaccat gttgcgtcac cagtaaatcc
     1441 gcgctgagtt tatgacgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
     1501 tccacccgac gcgcagaaag catacgcagc agacgctcac ggctgagaat ttcgcccgga
     1561 ttagtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcgcca
     1621 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagtag
     1681 tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc gccagagcag atttttcacc
     1741 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
     1801 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
     1861 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggtaa gttgatatcc
     1921 agcagaatta aatctaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
     1981 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
     2041 ggcttatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
     2101 tatctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta caggggttat
     2161 tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg tactggcgct
     2221 ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg ttgggatcaa
     2281 agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga
     2341 atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat gccatgactg
     2401 tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga gcaacgacat
     2461 ttaccagacc tttattgccc actccattga tacacctgaa aaattcgaag ccaaacgcgc
     2521 ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa cctgtcgctc
     2581 ctgccataac tacgatgcga tggatcatgc caggcagcat cctgaagcgg cacgtcagat
     2641 gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta ttgcccacca
     2701 gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa
     2761 tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg cgaaaggcga
     2821 taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg
     2881 cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc agcgtgtact
     2941 cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca
     3001 ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga gcaagttgca
     3061 ggccaccgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga tctgggccta
     3121 tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg aaatcgccca
     3181 ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta ccagtctcga
     3241 taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg acaccgcagg
     3301 taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct ctttcaggca
     3361 tcacgtcggc gttttctggc acaacttggc ggcttaaccg tcgccgggat gctggggccg
     3421 tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc tgtcatctcg
     3481 aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgatggt gaaggatggt
     3541 cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat gatcgccgga
     3601 ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg cgtggactgg
     3661 ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt tgtgcgcgtg
     3721 agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtaca gaaaactcac
     3781 gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct
     3841 tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat
     3901 tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcaat ggaagtgtat
     3961 gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt gctgtggggc
     4021 tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa
     4081 tattacgagc agctaaaagc gaaagtcgcc gccggtgaag ttgaggtcat cagcatcgat
     4141 ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt
     4201 aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcac atacgctgta cagtgaaaac
     4261 ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt cctgccgtat
     4321 ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc
     4381 attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt
     4441 attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg
     4501 gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ctggcactac
     4561 aacggcgcag gcacgccggg gcgtaaaggc gttattctga gtggtttctc cggctctacg
     4621 tcgattccgc ctgttcacga caacagtgac tacaaaggct acagcagcac tattccgatt
     4681 gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga tcaactggaa cggtaaatcg
     4741 gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa ctaacccatt ccatcgccat
     4801 cagcagatca accgcattat tgaaggctgg cgcaagctgg aaacggttat cgccatagat
     4861 aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc tgcctgcgac cacgcagttt
     4921 gagcgtaacg atctcgacca gtacggcaac cactccaacc gtggcattat cgccatgaaa
     4981 caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg atattttccg cgagctgtgc
     5041 cgtcgcttta atcgtgaaga agcctttacc gaagggctgg acgaaatggg ctggctgaaa
     5101 cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg gcgttcatct gccagcgttt
     5161 gatgacttct ggaataacaa agagtacgtc gagtttgacc atccgcagat gtttgttcgc
     5221 caccaggcat tccgcgaaga tccggatctc gaaccgctgg gcacgccgag tggcctgatt
     5281 gagatctact cgaaaaccat cgccgatatg aactacgacg attgtcaggg gcatccgatg
     5341 tggtttgaga aaatcgaacg ctcccacggt gggcccggct cgcagaagta tccgttgcat
     5401 ctgcaatctg tgcatccgga tttccgactt cactcgcagc tatgtgagtc ggaaacgctg
     5461 cgtcagcaat atacggtagc gggtaaagag ccagtgttca ttaacccgca ggatgccagc
     5521 gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta acgctcgcgg tcaggtgttg
     5581 gcaggagcag tagtttctga ccgctatgca cc
//
LOCUS       DAJTNV010000166         5582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNV010000166 DAJTNV010000000
VERSION     DAJTNV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(1..279)
                     /locus_tag="OO199_003778"
     CDS             complement(1..279)
                     /locus_tag="OO199_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537647.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO199_003779"
     CDS             220..405
                     /locus_tag="OO199_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537648.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO199_003780"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO199_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5537649.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO199_003781"
     CDS             complement(1254..1670)
                     /locus_tag="OO199_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537650.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO199_003782"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO199_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="OO199_003783"
     CDS             1837..2877
                     /locus_tag="OO199_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5537651.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="OO199_003784"
     tRNA            complement(2905..2994)
                     /locus_tag="OO199_003784"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="OO199_003785"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="OO199_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5537652.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="OO199_003786"
     tRNA            3986..4061
                     /locus_tag="OO199_003786"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="OO199_003787"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="OO199_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1505 a         1322 c         1292 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
     5581 at
//
LOCUS       DAJTNV010000167         5559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNV010000167 DAJTNV010000000
VERSION     DAJTNV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.168"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..641
                     /locus_tag="OO199_003788"
     CDS             <1..641
                     /locus_tag="OO199_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5537653.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO199_003789"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO199_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5537654.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO199_003790"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO199_003790"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5537655.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DAJTNV010000168         5530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNV010000168 DAJTNV010000000
VERSION     DAJTNV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(22..588)
                     /gene="satP"
                     /locus_tag="OO199_003791"
     CDS             complement(22..588)
                     /gene="satP"
                     /locus_tag="OO199_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5537656.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(737..1450)
                     /gene="msyB"
                     /locus_tag="OO199_003792"
     CDS             complement(737..1450)
                     /gene="msyB"
                     /locus_tag="OO199_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5537657.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1476..1880)
                     /locus_tag="OO199_003793"
     CDS             complement(1476..1880)
                     /locus_tag="OO199_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5537658.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2257..4173
                     /gene="dnaK"
                     /locus_tag="OO199_003794"
     CDS             2257..4173
                     /gene="dnaK"
                     /locus_tag="OO199_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5537659.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4262..5392
                     /gene="dnaJ"
                     /locus_tag="OO199_003795"
     CDS             4262..5392
                     /gene="dnaJ"
                     /locus_tag="OO199_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5537660.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         1465 a         1363 c         1456 g         1246 t
ORIGIN      
        1 agcgggggga tctcaagaca attagtggga ttcaccaatc ggcagaacgg tgcgaccaaa
       61 ctgctcgttc agcacttcac ccatcgccag atagattgcg ctggcaccgc agatcagacc
      121 aatccagccg gcaaagtgga tgatcgcggc attaccggca atgttaccga tcgccagcag
      181 ggcaaacagc acggtcaggc taaagaaaac gaattgcagg acgcgcgcgc ctttcagagt
      241 gccgaagaac ataaacagcg taaatacgcc ccacagaccc aggtagacac caaggaactg
      301 tgcatttggc gcatcggtca gacccagttt cggcatcagc agaatcgcaa ccagcgtcag
      361 ccagaaagaa ccgtaagagg tgaatgcggt taaaccgaaa gtgttgcctt ttttgtactc
      421 cagcagacca gcaaaaattt gcgcgatgcc gccgtagaaa atgcccatgg caagaataat
      481 accgtccaga gcgaaataac ccacgttgtg caggttaagc agaatggtgg tcatgccgaa
      541 gcccatcagg cccagcggtg ccggattagc caacttagtg ttgcccataa ttcctcaaaa
      601 atcatcatcg aatgaatggt gaaataattt ccctgaataa ctgtagtgtt ttcagggcgc
      661 ggcataataa tcagccagtg gggcagtgtc tacgatcttt tgaggggaaa atgaaaattt
      721 tccccggttt ccggtatcag acctgagtgg cgctaaccat ccggcgcagg caggcgattt
      781 gcagtacggc tggaatcgtc acgcgatagg tgctgccgct gaccgcttta accccattta
      841 atgctgcgcc cacagggcct cccagccccg cgccgcgcag caaaccatgc ccaagtacgc
      901 tcattgctgc gtgggtgcgt aaaatgcggg tcagttggct ggaaagcaaa tgcgacacac
      961 cttttgccaa taatttgtct ttcatcagca gcggcagcag ctcttccagc tcattcaccc
     1021 tggcatcgac cgcgtgcaga aactcctgct tatgttcctc gtccattttc ttccaggtat
     1081 tacgcagaaa ttgttccagt aactgttgct caatttcaaa cgtagacatc tctttgtcgg
     1141 ctttcagctt caatcgcttt gaaacatcga gcaaaatggc ccgatacaat ttaccgtgtc
     1201 cgcgcagttt gttggcgata ctatcgccac caaaatgctg taattctccg gcaatcagct
     1261 gccagttgcg gcgatgttgc tcgggatgcc cttccatcga tttaaacagt tcgttgcgca
     1321 tcagtatgct ggagaggcga gttttgcctt tttcattatg ggtgagcaat cgggcgaaat
     1381 ttgccaactg ttcctcacta caatgctgaa gaaaatccag atctgaatca ttcaggtaat
     1441 taacattcat tttttgtggc ttctatattc tggcgttagt cgtcgccgat aattttcagc
     1501 gtggccatat ccgatgagtt caccgtatga ccggaaaagg tgatttttga gacgcagcgt
     1561 ttattgtcgt tatcgctgtt aatgttgatc cagtcagtgg tttgcccttc ttttatttct
     1621 gaaggaatat tcaggctctg actggcgcta cgggcggctt tgaaataaac cgatgcacca
     1681 cttaactgta aatcgccatg gtcggcagag agttgtatgc gtttcacaat gcgacaaaca
     1741 ggaagtttca gcgccagatc gttggtttcg ttacgcggca ttgcaatgac gccgaggagt
     1801 ttatggtcgt ttgcctgcgc cgtgcagcac agcatcaggc taatcgccag gctggcggaa
     1861 atcgtaaaaa cggatttcat aaggattctc ttagtgggaa gaggtagggg gatgaatacc
     1921 cactagttta ctgctgataa agagaagatt caggcacgta atcttttctt tttattacaa
     1981 ttttttgatg aacgccttgg ctgcgattca ttctttatat gaataaaatt gctgtcaatt
     2041 ttacgtcttg tcctgccata tcgcgaaatt tctgcgcaaa agcacaaaaa atttttgcat
     2101 ctcccccttg atgacgtggg ttacgacccc atttagtagt caaccgcagt gagtgagtct
     2161 gcaaaaaaat gaaattgggc agttgaaacc agacgtttcg cccctattac agactcacaa
     2221 ccacatgatg accgaatata tagtggagac gtttagatgg gtaaaataat tggtatcgac
     2281 ctgggtacta ccaactcttg tgtagcgatt atggatggca ccattcctcg cgtgctggag
     2341 aacgccgaag gcgatcgcac cacgccttct atcattgcct atacccagga tggtgaaact
     2401 ctggttggtc agccggctaa acgtcaggca gtgacgaacc cgcaaaacac cctgtttgcg
     2461 attaaacgcc tgattggccg ccgcttccag gacgaagaag tacagcgtga tgtttccatc
     2521 atgccgttca aaattattgc tgctgataac ggcgacgcat gggtcgaagt taaaggccag
     2581 aaaatggcac cgccgcagat ttctgctgaa gtgctgaaaa aaatgaagaa aaccgctgaa
     2641 gattacctgg gtgaaccggt aactgaagct gttatcaccg taccggcata ctttaacgat
     2701 gctcagcgtc aggcaaccaa agacgcaggc cgtatcgctg gtctggaagt aaaacgtatc
     2761 atcaacgaac cgaccgcagc tgcgttggct tacggtctgg acaaaggcac tggcaaccgt
     2821 actatcgcgg tttatgacct ggggggtggt actttcgata tttctattat cgaaatcgac
     2881 gaagttgacg gcgaaaaaac cttcgaagtt ctggcaacca acggtgatac ccacctgggt
     2941 ggtgaagact tcgacagccg tctgatcaac tatctggttg aagaattcaa gaaagatcag
     3001 ggcattgacc tgcgcaacga tccgctggca atgcagcgcc tgaaagaagc ggcagaaaaa
     3061 gcgaaaatcg aactgtcttc cgctcagcag accgacgtta acctgccata catcactgca
     3121 gacgcgaccg gtccgaaaca catgaacatc aaagtgactc gtgcgaaact ggaaagcctg
     3181 gttgaagatc tggtaaaccg ttccattgag ccgctgaaag ttgcactgca ggatgctggc
     3241 ctgtccgtat ctgatatcga cgacgttatc ctcgttggtg gtcagactcg tatgccaatg
     3301 gttcagaaga aagttgctga gttctttggt aaagagccgc gtaaagacgt taacccggac
     3361 gaagctgtag caatcggtgc tgctgttcag ggtggtgttc tgactggcga cgtaaaagac
     3421 gtactgctgc tggacgttac cccgctgtct ctgggtatcg aaaccatggg cggtgtgatg
     3481 acgacgctga tcgcgaaaaa caccactatc ccgaccaagc acagccaggt gttctctacc
     3541 gctgaagaca accagtctgc ggtaaccatc catgtgctgc agggtgaacg taaacgtgcg
     3601 gctgataaca aatctctggg tcagttcaac ctagatggta tcaacccggc accgcgcggc
     3661 atgccgcaga tcgaagttac cttcgatatc gatgctgacg gtatcctgca cgtttccgcg
     3721 aaagataaaa acagcggtaa agagcagaag atcaccatca aggcttcttc tggtctgaac
     3781 gaagatgaaa tccagaaaat ggtacgcgac gcagaagcta acgccgaagc tgaccgtaag
     3841 tttgaagagc tggtacagac tcgcaaccag ggcgaccatc tgctgcacag cacccgtaag
     3901 caggttgaag aagcaggcga caaactgccg gctgacgaca aaactgctat cgagtctgcg
     3961 ctgactgcac tggaaactgc tctgaaaggt gaagacaaag ccgctatcga agcgaaaatg
     4021 caggaactgg cacaggtttc ccagaaactg atggaaatcg cccagcagca acatgcccag
     4081 cagcagactg ccggtgctga tgcttctgca aacaacgcga aagatgacga tgttgtcgac
     4141 gctgaatttg aagaagtcaa agacaaaaaa taatcgccct ataaacgggt aattatactg
     4201 acacgggcga aggggaattt cctctccgcc cgtgcattca tctaggggca atttaaaaaa
     4261 gatggctaag caagattatt acgagatttt aggcgtttcc aaaacagcgg aagagcgtga
     4321 aatcagaaag gcctacaaac gcctggccat gaaataccac ccggaccgta accagggtga
     4381 caaagaggcc gaggcgaaat ttaaagagat caaggaagct tatgaagttc tgaccgactc
     4441 gcaaaaacgt gcggcatacg atcagtatgg tcatgctgcg tttgagcaag gaggcatggg
     4501 cggcggtggt tttggcggcg gcgcagactt cagcgatatt tttggtgacg ttttcggcga
     4561 tatttttggc ggcggacgtg gtcgtcaacg tgcggcgcgc ggtgctgatt tacgctataa
     4621 catggagctc accctcgaag aagctgtacg tggcgtgacc aaagagatcc gcattccgac
     4681 tctggaagag tgtgacgttt gccacggtag cggtgcaaaa ccaggtacac agccgcagac
     4741 ctgtccgacc tgtcatggtt ctggtcaggt gcagatgcgc cagggattct tcgctgtaca
     4801 gcagacctgt ccacactgtc agggccgcgg tacgctgatc aaagatccgt gcaacaaatg
     4861 tcatggtcat ggtcgtgttg agcgcagcaa aacgctgtcc gttaaaatcc cggcaggggt
     4921 ggacactgga gaccgcatcc gtcttgcggg cgaaggtgaa gcgggcgagc atggcgcacc
     4981 ggcaggcgat ctgtacgttc aggttcaggt taaacagcac ccgattttcg agcgtgaagg
     5041 caacaacctg tattgcgaag tcccgatcaa cttcgctatg gcggcgctgg gtggcgaaat
     5101 cgaagtaccg acccttgatg gtcgcgtcaa actgaaagtg cctggcgaaa cccagaccgg
     5161 taagctattc cgtatgcgcg gtaaaggcgt caagtctgtc cgcggtggcg cacagggtga
     5221 tttgctgtgc cgcgttgtcg tcgaaacacc ggtaggcctg aacgaaaagc agaaacagct
     5281 gctgcaagag ctgcaagaaa gcttcggtgg cccaaccggc gagcacaaca gcccgcgctc
     5341 aaagagcttc tttgatggtg tgaagaagtt ttttgacgac ctgacccgct aacctcccca
     5401 aaagcctgcc cgtgggcagg cctgggtaaa aatagggtgc gttgaagata tgcgagcacc
     5461 tgtaaagtgg cggggatcac tccccgccgt tgctcttact cggactcgta agccgtgaaa
     5521 acagcaacct
//
LOCUS       DAJTNV010000169         5401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNV010000169 DAJTNV010000000
VERSION     DAJTNV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO199_003796"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO199_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5537661.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO199_003797"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO199_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5537662.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="OO199_003798"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="OO199_003798"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5537663.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="OO199_003799"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="OO199_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="OO199_003800"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="OO199_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5537664.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="OO199_003801"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="OO199_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5537665.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1525 a         1233 c         1114 g         1529 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 c
//
LOCUS       DAJTNV010000170         5399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNV010000170 DAJTNV010000000
VERSION     DAJTNV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..497
                     /locus_tag="OO199_003802"
     CDS             <1..497
                     /locus_tag="OO199_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5537666.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO199_003803"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO199_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO199_003804"
     CDS             2500..3114
                     /locus_tag="OO199_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5537667.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO199_003805"
     CDS             3114..3443
                     /locus_tag="OO199_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5537668.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO199_003806"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO199_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5537669.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="OO199_003807"
     CDS             4530..4841
                     /locus_tag="OO199_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5537670.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4976..5374
                     /locus_tag="OO199_003808"
     CDS             4976..5374
                     /locus_tag="OO199_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5537671.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1180 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggca
//
LOCUS       DAJTNV010000171         5347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNV010000171 DAJTNV010000000
VERSION     DAJTNV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(71..553)
                     /locus_tag="OO199_003809"
     CDS             complement(71..553)
                     /locus_tag="OO199_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537672.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(621..2570)
                     /locus_tag="OO199_003810"
     CDS             complement(621..2570)
                     /locus_tag="OO199_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5537673.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2655..2990)
                     /locus_tag="OO199_003811"
     CDS             complement(2655..2990)
                     /locus_tag="OO199_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5537674.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2990..3346)
                     /locus_tag="OO199_003812"
     CDS             complement(2990..3346)
                     /locus_tag="OO199_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5537675.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3346..4842)
                     /locus_tag="OO199_003813"
     CDS             complement(3346..4842)
                     /locus_tag="OO199_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5537676.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4839..5000)
                     /locus_tag="OO199_003814"
     CDS             complement(4839..5000)
                     /locus_tag="OO199_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5537677.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5010..>5347)
                     /locus_tag="OO199_003815"
     CDS             complement(5010..>5347)
                     /locus_tag="OO199_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537678.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1273 a         1561 c         1149 g         1364 t
ORIGIN      
        1 agaaggtaaa gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc
       61 ggcttatata ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca
      121 ctcccgactc gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta
      181 caaaaaactg gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa
      241 aatcatcatc tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg
      301 caaaatcaaa tacacctata cttttgccga attcataaga agcttttctc aatataatcg
      361 caccgctggc agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca
      421 agtcaacgtc attatctgca gatgttttaa cgttactaat aaatcgtgaa acaatctcct
      481 catttgttag tattttcgcc tgagcacagc aagaggttaa tattgcaacc gtcattaaaa
      541 atggctttat cacaaaacaa actccattgt tagcatgtta cgcgataatt ttgcctcatc
      601 tttcaccaca aaaaaagaac tcacctttta aatggagaat acccaacatc atgggtgatt
      661 ttcataaagg gatcgcctgt tttcggcaaa tcgataacgc gcattccagg cggcgcgttg
      721 tcaaacgtca ccttaagttc gctgcgggtt gccggtgaag atgcaaggct taacagctga
      781 ttcctttcca gtgatacatt cggggtgtag cgtttatcgg gagataaata cccatcacca
      841 cgtggcatct gccatccggt catgtcatag acaaaatcat ggaactgttt gccccactcg
      901 tcgaacttat cattcagacc aaatccactg ttcagtgcat cagccacaaa atttttaaca
      961 agccagggat gttctttttc aaactcctgc gccatcatgc ccagttcaag caatccacca
     1021 atcagactga tctttccgag gccctttaat ttcagccctg tttttcctgc cgccgttgtc
     1081 cattgattct gggcgactgt tgccgcacga atacttgcca ccgccttcac accaatatac
     1141 agggcagaaa ccgttgctat tgtttttact gcctgttccc atccccccat tgctctgaca
     1201 acctgatcaa cctcctgcca gacttttttc acaaccggac cgaccgtttc ccagttatca
     1261 attatgagat atgcaccacc gacgagaagc gcaattaatc cctttgccgg cgtcatattc
     1321 atcacgctac ccatgatttt tgttatgcgt gtcaatgtgc caatcgccac gcccatcgtc
     1381 agtaatgccg caccagtttt cgcgatggtt tttacgacct caggattttc tctgacaaac
     1441 gtgcgcactt cttccagaaa aggcttcatc tcttttatgc cttcattgag tgaaggcagg
     1501 aaggtttccc ccagcgtgga agaaatcgca ttgatctggt tctgcagcag caacaactga
     1561 ttttctgtcg tcgcggcacg ggcggcatat tctttctgca ttgagccgcc atactgctgc
     1621 gcatccgcca cacgattaaa gttggttcgc aacaaatcca gattcgtgag cagcggcgca
     1681 atggccccca gggattcctt cccgaacagc gctttcagca cagcagcctg tttttctttc
     1741 ggcacttttg ccagagaatc cagtacgtgc agcatggccc cgcgggcgtc tttctgcata
     1801 tccgccgcca gttttttcgg gctaatgcgc aacgagcgga gcactttttt ctgcgatttg
     1861 gtggcagaat ccctcgctgt cagcgacaac ataaaattct ttatccccgt cgcagcaatt
     1921 tctgactcca cccccattcc ggcaatggtt gcccccattg cggcaatctc tccggaggcc
     1981 acacccgcaa cactgcctaa agggccaata cgggtcacaa catcagaaat ctttttcgca
     2041 cttgcaggac cggtattgcc aagatagtta atcttatccg caagccctgc cacctctccc
     2101 tgtgtcagtt taaaggcagt gcgccactgc gccatcatct gaccggattc ttctgccgtg
     2161 gtgtcaaagg ccacgcccat cttcacggca tcgtcagtaa actgcatcag ctcgtcacgg
     2221 gcgataccag cctgaccacc cgccgccaca atttcggcga taccttccgc cgacatgggc
     2281 agttctgttg acaggtcgcg cacctgctcc gtcattgcct taaacgcttc cggcgtatcc
     2341 agaccatcca ccactttccg gacatccgcc atttttgatt caagggcaat ggcagatttg
     2401 accgggagcg ccagcgcccc cagtactgcg gttccggcac ccgctgcgcc cagtgacagg
     2461 ctggcaaact cctttttaaa acctttcagc tggcgctgca ttcccttcag cggtgccgat
     2521 accttatcca cggcagtgat gatcgccttc agctgaaaac tgtcagccat gcttcatctc
     2581 ctcgttaatg cggacggcct cggcttccag ttctgtaaac tgcgaaacag ccacccgtcg
     2641 cagctccagt ggattcagtt tccagaacca ggcaacattg tagagtcgtt tccggaggtg
     2701 cttcccgtct ccgactgggt aaaaaaacgc aggatctgca tgctggtctt aaaaatatcc
     2761 agttttgcca tctgcgccgc cgatgaacac gggatccccg ccagcaacgg gatatatttc
     2821 agtgccacct ggctgtccag tttaatactg ccttcaccgg aaataatgaa aggaaaaccc
     2881 agcgcctcga tttcgtcata cgtgggctcg cgcaactcca gcacatgcag catttcgtta
     2941 tgcgctgtca ccggtttttt taaaactatt tctgtaacgt tcattgccat tactgatacc
     3001 ctccctcttc gccgtggaat tcaagatctg ccgtgccttc ttcggcatta tgattagcct
     3061 ctccgtgcag ccatgccgcc gaaagcacat acaccatgcc gtttgccagc tcagcggtaa
     3121 tcgtcatctg gtctgaggtg gtaattttgt cgaccggaaa gtttttgggc actttaaacg
     3181 tgcccttaac gtaaggtgac cgccaggtct ccttgtaatc cacatcccct gccatgccga
     3241 cgatgtcatc ccggacattg gtgttcatcg gcacttcaat gccacctgtc agcgataact
     3301 gctgaccatc cactttaaaa aaacaggttc cggcgatctt tgccattatg ctgactcctc
     3361 tgaatactga agacggaact ggttaaccac cgcaaagaca cgtaactgat taacataatc
     3421 cggcgggaac agcgtattca gtcggttcgg gttatctgca tcacgctcaa ctatcagata
     3481 ctgtttaaac agatcgtaat tttccacaat accggcacgc tccatctgac gatatgtcgc
     3541 cagaagttcc cctttgataa cggcaggagt gacaatcgcc tgccccggac caaaacgggt
     3601 accatcattt gccagcttgt gacgtccgta cttgcttgtg atgaccgatt tcagtttgcg
     3661 aagaacatat gcactggtat gcagagtttc actgtccaga tagctgttgt ctgccacgcc
     3721 atacgcattc tttttgtagg tggttacgga acgctggatc tgcaacgtgc cgccttccac
     3781 ataagccgtc gccacgccgt gagataaaag ggtctgctgc tctgtcatga tgaatcgctt
     3841 acctttcggt gccggaagca tccccaccag ctcccccgtc tgtgtcggac gggcaggatc
     3901 attccggata aataccgctt cacgggcaag gcgactggca accagttcat cgacaggcga
     3961 ctgggtttct ttttcgtaac cggcaagcgt gatatgttgc tgattatgca tatctccggc
     4021 accaaccagc tctgacagcg ttcccagttt tgcggtatag acatgcccgt ataactggcg
     4081 cgcataactc cagcgaccgc tgctgtcatt catttcggtc atcatcatat tgatggaggc
     4141 ggcatcgttg aacggcagac cgataaagtc gaatgcctca tcgcccatag cagcaacagc
     4201 ggcggtaaga tcaggcgctc cgctaccggc agcaccgact tccgtcacca cccgaagccc
     4261 tgctggcaaa atctcaccac caccagaacc ataataattc agacagaccg gtaactcatt
     4321 accatacagc cccttatgac gggcagtcag cgtcaccacg cctgcatcag atgacgccgt
     4381 aaacggcagg gtgataaccc cgtttaccgc ttccttaatc gcggtggcaa ccgcagtggc
     4441 atcatcgcca ttcaccacag gcacctgtac acgggagcgc ccgacataca gactcagggt
     4501 gccgctttcc tctgcttctc cggcaaccgt caccctgacc gtcgccgccg cccctctggc
     4561 ttccggtact gcaataacat acagttcacc gaaaggatct gtctgacggt agacttccac
     4621 catacgcgcc agctggctcc ccgccccaca aatctggcgg gcataatctg ccgatggcat
     4681 cagcaccagg ctgttaacct caatggctgc atcgttgctg gcatgcccga tcagtaatgc
     4741 aggcgcgctg gtcaccgctg tatttgccgc agaattgttc atctcggcgt agaacaacgg
     4801 caccagcgta ttcgacggaa cagtattaaa acttatcgtc atgatttttt agccttatcc
     4861 tgtttaacac gtaccacatc acccgccgct atacggcgga accagtagct gctttcttcc
     4921 acatttcgcc cttcggaagg caaaaggtct ccacgggcag ggtcaggaac cgaccgccct
     4981 ttcaggggtt tcacaaacat gagggactct taattctgag ggaaacgcat ttccagatgg
     5041 tgctcaatgt caccatccgg gccagtacca ggatcgatgt aatccacatc aatactcagc
     5101 agggataaat ccggcagggc attcacgtcc tctgcctgtc gcgtgtcttc ttccgtgatt
     5161 tcatacttcg ccgtaaaatc aaactggtaa tacagttcgt aacggttcag atccagcagg
     5221 gtgccaccga cataaacaat ctcgccccct tgcgggtccg gcatccatcc aagcagggct
     5281 ttccagagtt ctctccggac atcatgaacg gcgtcatagg ctgcccactg ccctttttca
     5341 tcacgct
//
LOCUS       DAJTNV010000172         5328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNV010000172 DAJTNV010000000
VERSION     DAJTNV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO199_003816"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO199_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5537679.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="OO199_003817"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="OO199_003817"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5537680.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="pgeF"
                     /locus_tag="OO199_003818"
     CDS             1872..2603
                     /gene="pgeF"
                     /locus_tag="OO199_003818"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5537681.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..5306
                     /gene="clpB"
                     /locus_tag="OO199_003819"
     CDS             2733..5306
                     /gene="clpB"
                     /locus_tag="OO199_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5537682.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1309 a         1286 c         1452 g         1281 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcaacag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggttacgatc
     5161 cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa aacccactgg
     5221 cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg gaagttaatg
     5281 aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAJTNV010000173         5280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTNV010000173 DAJTNV010000000
VERSION     DAJTNV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.174"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..682)
                     /locus_tag="OO199_003820"
     CDS             complement(<1..682)
                     /locus_tag="OO199_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5537683.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLP"
     gene            complement(697..1434)
                     /locus_tag="OO199_003821"
     CDS             complement(697..1434)
                     /locus_tag="OO199_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5537684.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(1322..2788)
                     /locus_tag="OO199_003822"
     CDS             complement(1322..2788)
                     /locus_tag="OO199_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5537685.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(2788..4410)
                     /locus_tag="OO199_003823"
     CDS             complement(2788..4410)
                     /locus_tag="OO199_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCR5537686.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(4413..4985)
                     /locus_tag="OO199_003824"
     CDS             complement(4413..4985)
                     /locus_tag="OO199_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5537687.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(4969..>5280)
                     /locus_tag="OO199_003825"
     CDS             complement(4969..>5280)
                     /locus_tag="OO199_003825"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5537688.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         1114 a         1513 c         1333 g         1320 t
ORIGIN      
        1 gcggcaaatc cccctcatct cctgtaaccg atgcgggagc cgactccacc agttcgttca
       61 tggtgtcagc gtcacccgtt ttgatggccg tgcgcatgcg ggtccaccag cttttctttt
      121 gatttgccat tgtgtctctg tctccaattg cacaacgatt tccggctctg cctttgggga
      181 caagagccac atggtttccg gtaatatcga cctgctcggc tttacctggc tcggtctgct
      241 cgtactccgc gtcatagccg cacgacactt cacgcaggcc atcttcgata agctgaatgg
      301 cgttttcgtc tttgacgata aggtcagcca gcatcaaatc agagtggtca cccgtcccgc
      361 gccgcacatt ctgaagatgc ccgacagcaa gctctttcca gttctcggga tttaccagcc
      421 gcacattccc gttttcatct tcaggatgca ggatcgtgat gctcatccct tcgaatgagg
      481 cgagcgtggc cggatggaat acctgctcag gagagcgcgt tacgactatc tcaccgagct
      541 tgtcgggttt gaggtttggc agatcggcag cgccgtagag ctgtttaccc gttcgaccta
      601 tcggcacgtc tttgcacagc agcgagccgt cagccagctg atagcgggtt tcccccagcc
      661 gggtattgaa aaaatatttc atggtttacc tgcgattcag gcgagataag aatgagggtt
      721 gggaaaaacg atttctttat aacagcgaca attcgggagc tcgccagcgt gaccggtcat
      781 gccatcaagc gttggaggtt tgccccattc gacaaactta ccttccatct cccgatgaga
      841 atgccggacg tcgccatctt cggctgtacg ccagatataa ccattcgagc cgattgacag
      901 cgcacgcgcc tgatcgagcg cgccggttgc acgtccaagc tcggtacggg cgataaggtt
      961 cgctcgtgag cgtgacacgt caccggacgc tgctatctct ttcgcgaatg gttcagcgcg
     1021 gccaccagcc acaacggcct cgatggcctt gttctgaatg tcatacaccc gatcggcggc
     1081 ctcaagaggc agtgacttga tatacttaat ttgctcggcg acgatggatt tcatcacctg
     1141 gcctaccggg gcgcggtcga ccatgttgcg cagctctgcg ctgatgttcc ggctgtgctg
     1201 acgccactgc ttttcattct ggcgtgcaat gtcggcggta aagttctcag caaccttcgt
     1261 cgcccagggg gtgatgattt cgctgtagcg ctccagcgca tccattattt cggtaacgct
     1321 atcatttgaa ccatcgtagc gcccatttac gatatccccg accgcccgcg ctatctgccg
     1381 taggctcgtt cgatatcgga tctccgcctg gcggctctgg cggtttgtcg ccaagttcgc
     1441 cgatgcctgg cggcgcttcg tcttcggcat tctcgatatc ctcgtcggta atggatgccc
     1501 cgatgccggt gacgtcagag ttttcgcgca ggtcggtcat cgccgcctta cgcgtcatca
     1561 atccgtcgcc cagcgcggta ctaatcgcgt tggtggtgtt tacggccacc gttgatcggt
     1621 caacgtctga catttgccat agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     1681 ttccgagttc cgagcggtgc ataatgtcca gtatccggcg catcggcagc cgtaagcggc
     1741 gctcctgcaa tgagctcacc cggtcgtaat agttggcgag gtctgcatca ccagtagaga
     1801 agcctttcgg ggattgaccg aacaaccgca ccagcgggat accaacggca ccgctaatct
     1861 gctcagcaaa ctgcgaaaga atgtcatcca gaccactaaa gctgtactgg tgggtttcga
     1921 acttatcccg cgagtccatg agcgtcatac cttcattgct ctggaactgg cggatcaggt
     1981 cgatgttctt cagcaacgcc tcgaacgccg ggcctccaag cgcgataagc tcgcgcaact
     2041 tctccacgct ataggtacgc agatgtgctt tgtagaccag ctgcgccgcg ccgacagtgg
     2101 cgctgtcgaa cgcagtaagc cgatcccaga tacgctctac aaccgacatt ccccattcgt
     2161 tttcggtcat cttctgctgg aatggcagcg tcaccccgtc gaagcgaatc aggcggctgt
     2221 gatggatgcg ccaggccggg atgcccgttg cagtggtcac cacgtcgtaa aactcaggtt
     2281 tgccgagatc tggccccatc tctttaatgc ggcgggtcag gaccgggtta atcatccagc
     2341 ggtcgagcgg gagaatgccc ttaaacttgc cttctccaat ggtttcgagc cgcagcgggg
     2401 tcattggtgc ctgcccctcg atcatgatga agccgaccgc gccgccgtag aggcgagacc
     2461 atttcagcac gtcgttcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     2521 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     2581 ccgggatagc atcgaccgct tcgccgataa tccaggacga acgataggac cattccacca
     2641 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     2701 tctgcatccc cacgcgggca acaaagttct catagccatc agcggtggcc tgtgccgttc
     2761 gccgcgtggc ttgtttgttt cgtgccatca ggcctgtctc cctagcagct cccagatgtt
     2821 cagggctgaa ttcattggcg cgtagctgat catcaccgag tcggcaaggt ttggcgaccg
     2881 ggttccatca ggctgtttat caataacgat ttttcccaca ccattaatgg aataggtcgg
     2941 ctgcgaaagc tcgatgatga gtttatcttt gagtgccatg ctactgctga ttgagatgat
     3001 ttcgtccggg ttgtaatcca taccttcaac cacggcgcgc caggtattct gaaaaagttt
     3061 acgtaaccgc caccagctct gggctttggc gttagcgaag aagtccttgt tcaggcgggc
     3121 ggcctgtccg ttgtcaccgc gcaccgcttc gtcgtccgga tcaaataccg cgccactacc
     3181 tcgaaacggt gtggcgagta ttgacggtcg gcgcgcagcg ttacgcagtt cgttgatagc
     3241 gcgtgcatcg ccgcgaacgc cagcgcccaa cccgtcctcg tcaaagcgaa actcttcgag
     3301 gttgtcctgt tcgcaaaagc cgaaaacctt ctcgacggac tgataaatgt cgctgcccac
     3361 accggaccat tcccgcacat tctccaggag gaagccatga cgggtggaaa aggaattttt
     3421 gtccctgcct tcgtcggcga cgtccatcgc gcccagtcgc ttgcctgttg gctggatgcc
     3481 cagcttgata tgcgcgtcga cagcagcctg tacccattcg gatggaatca ggacgccttc
     3541 cgctgatgca ctgtagttca ggtcaagttc ctgtgccacc accaccggat tgtcgatttt
     3601 ctcgcattcc ctgcgatacc actcttcatc cttgcgagga tcatcccgcc agtggaatgt
     3661 gaataccggt atcttcccgc catgacgctt ctgagcgaac gggttcgcca tgccgttaac
     3721 tgaactcagg tcaatacggc aacgcgtcgt ttgtgacaac gccgcatcaa tcagcagagg
     3781 acgctgaagg aatgcagact catcaaccag ataaagcgtg gtacggtcac cacggccaat
     3841 attatcgcca gcctcacctt tgataacggc accagtttca ggaaactcaa cgcgcatgta
     3901 tggtgcgtgc ttcttttcgt cccacgaacc tcgaaactct acaggtagtg tttccacgaa
     3961 cttgcgcgcc ttccagaaca atgctttcgg gtcaccggtg ctgtcgacgt attcctcttt
     4021 acgggagccg aaaccgataa ccatttcttt gttgaagaga caaagcgagc atgccagccc
     4081 gatcgcggtc caactgagcc ccatttcacg ggatttttcg gtaataccat tctcccgatt
     4141 accccagcgt tccataatcc agtggatcca ctcctcctgc ttagggaaga gtaaaaacgg
     4201 aatggtcacc ggcaggccat aatcaatatt acgcgggtcc gttgtcatgc cccagtcgat
     4261 gatgaactga gccggattgg ttcggtaaaa ctgttttagt gcaggcaata tttcaggatt
     4321 ctggcgaatg cgctgtaggc gttccatccg ccattcaaaa accatctggt aatcaggatg
     4381 tttaaaatcg aaggggaatg gtaacggcat acttagccca tcatttttct atacgcctct
     4441 gcagcctgct ccggcgttaa gttggtaatt tctgttctga ctggtcctcc gtcagcacca
     4501 gtcacttcat ttttgacatt gtctttaaac gcctgaacag aaacatgacg cccgagcaac
     4561 tcaaggtttt taaccttatc aggccatttg attttcttca ggagagcagc actatctgct
     4621 gatgccatct cgacgacatc cattcctgat agcgttgtac gccacacctt aggccagtct
     4681 ttaatctgct tcagttcgcc attggcaagc agaatgtcaa gcacgtccat ctgatcgatt
     4741 tcagtcagcc gtcgcaatac ataggcggcg tctataccta cctcttcatt gcgttcagcc
     4801 tttagctcag caatgcgatc tgctatgtca ggttttgaca ggttctcgct accagtggcg
     4861 cgagcagttt tctcgctgta acccgccctg atagctgcct gagtggcgtt taaatctttt
     4921 aaatattccc tgcagaacag ttcctgctta ggtgtgagct gtttcaaatc acacctccga
     4981 aacattgctc caatttctat gattaacgat gtcagcaatc atggtagtgg atacgccata
     5041 tctctttgct aaagacctaa gcccattacc tacactcccg cgcacatact cgcggcgaat
     5101 gcagatgaca tcgcactctt ttaatttcgc cattccgttc ttttcacctg aacggtcagg
     5161 gtatgagttt ctgcctcgcc ttgacctgtc acgattattg tcagcgacag tacctataat
     5221 cagatggtca ggattcacgc atgatggatt atcgcattta tgcctaacaa caaggtcctt
//
LOCUS       DAJTNV010000174         5161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTNV010000174 DAJTNV010000000
VERSION     DAJTNV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.175"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            9..218
                     /locus_tag="OO199_003826"
     CDS             9..218
                     /locus_tag="OO199_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5537689.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(390..719)
                     /locus_tag="OO199_003827"
     CDS             complement(390..719)
                     /locus_tag="OO199_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5537690.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(724..909)
                     /locus_tag="OO199_003828"
     CDS             complement(724..909)
                     /locus_tag="OO199_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5537691.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(906..3545)
                     /locus_tag="OO199_003829"
     CDS             complement(906..3545)
                     /locus_tag="OO199_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5537692.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3753..4742
                     /gene="ldhA"
                     /locus_tag="OO199_003830"
     CDS             3753..4742
                     /gene="ldhA"
                     /locus_tag="OO199_003830"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5537693.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4853..>5161
                     /gene="hslJ"
                     /locus_tag="OO199_003831"
     CDS             4853..>5161
                     /gene="hslJ"
                     /locus_tag="OO199_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5537694.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1305 a         1323 c         1204 g         1329 t
ORIGIN      
        1 tgtaccgaat gtttgccgat aaaggtttgg tagtcgcgca atatattcgt aaccgtcgtc
       61 tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg gcaggcatcg
      121 gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag caacgctttg
      181 ggatgacgcc aggcgagtat cgacgtaaat tccgctaact aattggcgtt gcatgtactg
      241 caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcga aaggcaattt
      301 gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc tttctcctgt
      361 agcgttatcc cgatattgcg gttttgctat taaaactttc gcacccattt accgtttatc
      421 ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc gagtttcgcg
      481 atatcatcta cagacacatt attttgcttt gcccgttgtt ggtaactcgc tttacgttcg
      541 gcattaattt cggttactaa tttctctgtt tcagcatccg ttttcagcgc aaccagataa
      601 ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt caatgccatt
      661 acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt cttcatcatc
      721 gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt ctgccttgat
      781 gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct taggtgcagc
      841 gacttcaatg cgaggcgtac agccaaccag cataaaagat gacgtcaacg cagccagtaa
      901 aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc cggcagacgt
      961 gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg ccacagcgta
     1021 aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc atcaacgcga
     1081 ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa gttaattttt
     1141 gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc cgcagtcata
     1201 ttgtctttca ccaccgcatc tgcggtatct ttatccagcc gcaaggtcat tggcccgcta
     1261 tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa cggcaatgca
     1321 ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac cagttcgctg
     1381 gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg taattgtttc
     1441 atcagcacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa gggttcttct
     1501 tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt caccagatta
     1561 atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg
     1621 gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg catccaggca
     1681 ctaccgcctt tcaacacgcc gtgccctccc gcgcggaatc cttgttcagg cgcagcagaa
     1741 aatgcaacct gagcatacag ttcaccatcg cgtaagttca tcttccagtc gggtggcacc
     1801 agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg cagacgaata
     1861 ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa atcgccttta
     1921 aagaggaaat aggtcggatt gcgcccatcg acgctaaatt ttaaggttga tggcggtaac
     1981 acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag
     2041 ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg cggcttttct
     2101 accgtcatcg taccgtattg taactggtcg aaaccggtgg agagatcggt cagcgtaatc
     2161 gtgctgtcat gccactcacc ttttcctgcg acatcccagg tggcattcat cggtgtaaaa
     2221 ctcccttttc cccagtagcg ccactgccag cggccagcgt cagggagaaa atcattcgcc
     2281 agcccatcca tatgcagcac gaaatcgccc agttcatttt catgcgcctg caaaatggcc
     2341 tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg ccagcggatt
     2401 tcatcgatgt ccagcgaatc gatgactctt ccctttgaac gaagtaacgc gcctggctca
     2461 aaggtcagcg ttgggtcaga cagacttcca cttagctgcg caggtaaacg ggcatataaa
     2521 atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagttgact gttatccatg
     2581 cttaattttc ctgggccaaa attaagcacc gcgttgccct tacccgcttg cccctgggtc
     2641 agcacattca gtcgtccgct gaccagagcg ttctcaagcc ctgcctgcca gttgtcgact
     2701 ttgacaccca gtcggccact caaaggaaaa cctgcataca gccagctcca gcgaccatcg
     2761 cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg atcgccgtta
     2821 tcccgtgcca gcacgatcaa ttgcccgcta ttttcctgcc agtccagctc ggcatccacc
     2881 agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac aggaagtcca
     2941 tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg attttcaaac
     3001 gcgacgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc ttgaaattta
     3061 actttttcgc cctgataacg cagttgctgg atatcagagg ttaatgcgag agagagtttt
     3121 ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca ggtgttaggc
     3181 agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc cgtctgcggc
     3241 aatttcgcca gacaagcaga atcaagttct accgtgccga cgttcaataa ccagcggctg
     3301 ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac cagataacgg
     3361 agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt
     3421 gtcccgagcg gtagccagat gtccgccagc gtgggaaccc acagcccgag cgtcatcagc
     3481 agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt atatttaccc
     3541 agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac gggtattgtg
     3601 gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcatagttgt tgatgaattt
     3661 ttcaatatcg ccatggcttt caattatatt tgaaattttg taaaatattt ttagtagctt
     3721 aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat agcacaaaac
     3781 agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag ctggaatttt
     3841 ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta
     3901 ttttcgtaaa cgatgacggc agccacccgg tgctggaaga gctgaaaaag cacggcgtta
     3961 aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgatgcg gcaaaagaac
     4021 tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct gaacacgcca
     4081 tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt acccgtgacg
     4141 ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg gcaggcgtta
     4201 tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc
     4261 tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg gagtatgtcg
     4321 atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg ctgacaccgg
     4381 aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg
     4441 tcaataccag tcgcggtgca ttgattgatt ctcaggcggc aattgaagcg ctgaaaaatc
     4501 agaaaattgg ttcgttgggt atggacgtct atgagaacga acgcgatctg ttctttgaag
     4561 ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc tgccacaacg
     4621 tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt atttctcaga
     4681 ctacgctgca aaacttaagc aatctggaaa aaggtgaaac ctgcccgaac gaactggttt
     4741 aatcttgcag ctcccctgca atccagggga gctgattcag ataatcccca atgacctttc
     4801 attctctatt cttaaaatag tcctgagtca gaaactgtaa ttgagaacca caatgaagaa
     4861 agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta atgacaaaat
     4921 tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg taaacggtaa
     4981 gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa tgatgatttc
     5041 cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg aactgacagc
     5101 caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg aactcgataa
     5161 c
//
LOCUS       DAJTNV010000175         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNV010000175 DAJTNV010000000
VERSION     DAJTNV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.176"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO199_003832"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO199_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5537695.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO199_003833"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO199_003833"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5537696.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO199_003834"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO199_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5537697.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO199_003835"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO199_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5537698.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO199_003836"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO199_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5537699.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1415 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtaaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTNV010000176         4963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTNV010000176 DAJTNV010000000
VERSION     DAJTNV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            9..695
                     /locus_tag="OO199_003837"
     CDS             9..695
                     /locus_tag="OO199_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5537700.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(673..1296)
                     /gene="tetR(B)"
                     /locus_tag="OO199_003838"
     CDS             complement(673..1296)
                     /gene="tetR(B)"
                     /locus_tag="OO199_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5537701.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1378..2583
                     /gene="tet(B)"
                     /locus_tag="OO199_003839"
     CDS             1378..2583
                     /gene="tet(B)"
                     /locus_tag="OO199_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5537702.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2696..3289)
                     /gene="tetC"
                     /locus_tag="OO199_003840"
     CDS             complement(2696..3289)
                     /gene="tetC"
                     /locus_tag="OO199_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5537703.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3422..3793
                     /locus_tag="OO199_003841"
     CDS             3422..3793
                     /locus_tag="OO199_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5537704.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3803..>4963)
                     /locus_tag="OO199_003842"
     CDS             complement(3803..>4963)
                     /locus_tag="OO199_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5537705.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1329 a          940 c         1075 g         1619 t
ORIGIN      
        1 aacttgcgat ggctaattta atacgaaaag aggttacctt tgagtcctca atagccgcga
       61 taggggcggc tatgtctgac atttcacgag ttaaaatact cagtgctttg atggatgggc
      121 gagcttggac ggccactgag ctaagttctg tggcgaatat atcagcttca acggcgagca
      181 gtcatttatc taaattatta gattgccagc taatcacagt agtagctcaa ggcaagcatc
      241 gttattttcg gctagcagga aaagatattg ctgaattgat ggaaagtatg atggggatct
      301 ccttaaacca tggcgtacat gccaaagttt ccacgccagt gcatttacga aaagcacgta
      361 cttgctatga tcatttagct ggcgaagttg ccgttaagat ctatgattcc ctttgtcaac
      421 agcaatggat cactgaaaat ggttcaatga tcacattaag tggtattcaa tattttcatg
      481 aaatgggaat tgacgttcct tccaaacatt cacgtaaaat ctgttgtgcg tgtttagatt
      541 ggagtgaacg ccgtttccat ttaggtgggt acgttggagc cgcattattt tcgctttatg
      601 aatctaaagg gtggttaact cgacatcttg gttaccgtga agttaccatc acggaaaaag
      661 gttatgctgc ttttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
      721 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      781 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      841 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
      901 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
      961 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1021 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1081 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1141 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1201 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1261 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1321 actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg
     1381 aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt
     1441 atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac
     1501 cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt
     1561 ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca
     1621 tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt
     1681 ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc
     1741 acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt
     1801 ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt
     1861 tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa
     1921 accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg
     1981 gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa
     2041 ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg
     2101 aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa
     2161 gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga
     2221 tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt
     2281 ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg
     2341 atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt
     2401 accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta
     2461 ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg
     2521 ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct
     2581 tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc
     2641 aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt
     2701 tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg
     2761 caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt
     2821 ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa
     2881 tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa
     2941 tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa
     3001 gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg
     3061 tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt aattgttttt
     3121 tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc gagatctcat
     3181 ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag ttaaccagtg
     3241 attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg atagatttaa
     3301 aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac aatcttaaga
     3361 acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata aagctaataa
     3421 catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag atgttctatt
     3481 atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga ataaagcggg
     3541 ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca cactggctag
     3601 ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac
     3661 tatccttgag atcgcattaa aatatcaatt tgattcccaa caatctttta cacgtcgatt
     3721 taagtacatt tttaaggtta caccaagtta ttatcggcgt aataaattat gggaattgga
     3781 ggcaatgcac tgagagatcc cctcataatt tccccaaagc gtaaccatgt gtgaataaat
     3841 tttgagctag tagggttgca gccacgagta agtcttccct tgttattgtg tagccagaat
     3901 gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt ctgactgtgt
     3961 tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca agccaacatg
     4021 ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag ctgctcgttc
     4081 ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag gtttcttcaa
     4141 tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt tcaacaggta
     4201 agttagttgc tagaacccat ggctcctttg ccgacgctga gtagatttta ggtgacgggt
     4261 ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga gatttataca
     4321 atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct aaagtctttg
     4381 agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc gctcctaggt
     4441 ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc ttctcaacgg
     4501 atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg gtgttactcg
     4561 gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa cattgctctg
     4621 aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg actgaagctc
     4681 gcaataccat aagtcgtttt tgctcacgaa tatcagacca gtcaacaagt acaatgggca
     4741 tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt cgctctttgt
     4801 ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt gttctcgctt
     4861 tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca agtaatgcgt
     4921 ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttc
//
LOCUS       DAJTNV010000177         4963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTNV010000177 DAJTNV010000000
VERSION     DAJTNV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.178"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO199_003843"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO199_003843"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5537706.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO199_003844"
     CDS             1020..1703
                     /locus_tag="OO199_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5537707.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO199_003845"
     CDS             1700..2161
                     /locus_tag="OO199_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5537708.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO199_003846"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO199_003846"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5537709.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO199_003847"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO199_003847"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5537710.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO199_003848"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO199_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5537711.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO199_003849"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO199_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5537712.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1171 a         1302 c         1238 g         1252 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tcc
//
LOCUS       DAJTNV010000178         4867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTNV010000178 DAJTNV010000000
VERSION     DAJTNV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.179"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..969
                     /locus_tag="OO199_003850"
     CDS             <1..969
                     /locus_tag="OO199_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5537713.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="OO199_003851"
     CDS             1550..2773
                     /locus_tag="OO199_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537714.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="OO199_003852"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="OO199_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5537715.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="OO199_003853"
     CDS             3742..4272
                     /locus_tag="OO199_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5537716.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4867)
                     /locus_tag="OO199_003854"
                     /pseudo
     CDS             complement(4314..4867)
                     /locus_tag="OO199_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1557 a          998 c          905 g         1407 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aatacatgtg
     4861 tgcgtaa
//
LOCUS       DAJTNV010000179         4846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNV010000179 DAJTNV010000000
VERSION     DAJTNV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..433)
                     /locus_tag="OO199_003855"
     CDS             complement(<1..433)
                     /locus_tag="OO199_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR5537717.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYN"
     gene            798..1913
                     /gene="malK"
                     /locus_tag="OO199_003856"
     CDS             798..1913
                     /gene="malK"
                     /locus_tag="OO199_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5537718.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            1985..3325
                     /gene="lamB"
                     /locus_tag="OO199_003857"
     CDS             1985..3325
                     /gene="lamB"
                     /locus_tag="OO199_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5537719.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            3477..4397
                     /gene="malM"
                     /locus_tag="OO199_003858"
     CDS             3477..4397
                     /gene="malM"
                     /locus_tag="OO199_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5537720.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            4626..>4846
                     /locus_tag="OO199_003859"
     CDS             4626..>4846
                     /locus_tag="OO199_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537721.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDY
                     FIYISDIFLNIKQRNEKNHAINMLREVSI"
BASE COUNT         1223 a         1222 c         1231 g         1170 t
ORIGIN      
        1 tgttataaat cagcgataac gcttcaacag cgattgggta agcaatcagc ttgccgttgt
       61 aacgtacggc atcccatgta aacggataca gcttgtcctg gaacgctttg tccggggtga
      121 tttcagccaa caggccagat tgagcgtagc caccaaagcg gtcgtgcgcc cagaagataa
      181 tgtcagggcc atcgccagtt gccgcaacct gagggaattt ctcttccagt ttatccggat
      241 gctcaacggt gactttaatt ccggtatctt tctcgaattt cttaccgact tcagcgagac
      301 cgttatagcc tttatcgccg ttaatccaga ttaccagttt accttcttcg attttggcga
      361 gagccgaggc ggaaaacatc atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg
      421 tttttatttt cataatctat ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg
      481 actctagttt ctttatacgg caacctcttt ccatcctcct tgcccctacg ccccaccgtt
      541 gctttgtgtg atctctgtta cagaattggc ggtaatgtgg ggatgcgcac ataaaatcgc
      601 cacgattttt gcaagcaaca tcacgaaatt ccttacatga cctcggttta gttcacagaa
      661 gccgcgttct catcctcccg cctcctcccc cataaaaaag ccagggggtg gaggatttaa
      721 gccatctcct gatgacgcat agtcagccca tcatgaatgt tgctgtcgat gacaggttgt
      781 tacaaagaga gaagggcatg gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg
      841 aggtcgtggt atcgaaagat atcaatctcg atatccatga aggtgaattc gtggtgtttg
      901 tcggaccgtc tggctgcggt aaatcgactt tactgcgcat gattgccggg cttgagacga
      961 tcaccagcgg cgacctgttc atcggtgaga aacggatgaa tgacactccg ccagcagaac
     1021 gcggcgttgg tatggtgttt cagtcttacg cgctctatcc ccacctgtca gtagcagaaa
     1081 acatgtcatt tggcctgaaa ctggctggcg caaaaaaaga ggtgattaac caacgcgtca
     1141 accaggtggc ggaagtgctg caactggcgc atttgctgga tcgcaaaccg aaagcgctct
     1201 ccggtggtca gcgtcagcgc gtggcgattg gccgtacgct ggtggccgag ccaagcgtat
     1261 ttttgctcga tgaaccgctc tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg
     1321 aaatctcccg tctgcataaa cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg
     1381 tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga cgccggtcgc gtggcacagg
     1441 ttgggaaacc gctggaactg taccactatc cggcagaccg ttttgtcgcc ggatttatcg
     1501 gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc
     1561 aggtggagct gccgatgcca aatcgtcaac aagtctggct gcctgttgaa agccgtgatg
     1621 tccaggttgg agccaatatg tcgctgggta ttcgcccgga acatctactg ccgagtgata
     1681 tcgctgacgt catccttgag ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc
     1741 aaatctatat ccagatccct tccattcgtc aaaacctggt gtaccgccag aacgacgtgg
     1801 tgttggtaga agaaggtgcc acattcgcta tcggcctgcc gccagagcgt tgccatctgt
     1861 tccgtgagga tggcactgca tgtcgtcgac tgcataagga gccgggcgtt taagcacccc
     1921 acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga
     1981 tagaatgatg attactctgc gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat
     2041 gtctgctcag gcaatggctg ttgatttcca cggctatgca cgttccggta ttggctggac
     2101 aggtagcggc ggtgaacaac agtgtttcca gactaccggt gctcaaagta aataccgtct
     2161 tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt caggaagtgt ggaaagaggg
     2221 cgataagagc ttctatttcg acactaacgt ggcctattcc gtcgcgcaac agaatgactg
     2281 ggaagctacc gatccggcct tccgtgaagc aaacgtgcag ggaaaaaacc tgatcgaatg
     2341 gctgccaggt tccaccatct gggcaggtaa gcgcttctac caacgtcatg acgttcatat
     2401 gatcgacttc tactactggg atatttctgg tcctggtgcc ggtctggaaa acatcgatgt
     2461 tggcttcggt aaactctctc tggcagcaac ccgctcctct gaagctggtg gttcttcctc
     2521 ttttgccagc aacaatattt atgactatac caacgaaacc gcgaacgacg ttttcgatgt
     2581 gcgtttagcg cagatggaaa tcaacccggg cggcacatta gaactgggtg tcgactacgg
     2641 tcgtgccaac ctgcgtgata actatcgtct ggttgatggc gcatcgaaag acggctggtt
     2701 attcactgct gaacatactc agagtgtcct gaagggcttt aacaagtttg ttgttcagta
     2761 cgctactgac tcgatgacct cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt
     2821 tgataacgaa aaatttgcct acaatatcaa caacaacggt cacatgctgc gtatcctcga
     2881 ccacggtgcg atctccatgg gcgacaactg ggacatgatg tacgtgggta tgtaccagga
     2941 tatcaactgg gataacgaca acggcaccaa gtggtggacc gttggtattc gcccgatgta
     3001 caagtggacg ccaatcatga gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca
     3061 gcgcaccggc gacaagaaca atcagtacaa aattaccctc gcacaacaat ggcaggctgg
     3121 cgacagcatc tggtcacgcc cggctattcg tgtcttcgca acctacgcca agtgggatga
     3181 gaaatggggt tacgactaca acggcgatag caaggttaac ccgaactacg gcaaagccgt
     3241 tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg
     3301 tgcccagatg gaaatctggt ggtaatagca aaacctgggc cggataaggc gtttacgccg
     3361 catccggcat aaaaacaggt tgtcattatc tgaaaggggc gaaagcccct ctgattatcg
     3421 ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga
     3481 aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttactggca agcgcgcctg
     3541 gaattagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc
     3601 catctgccgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa acccagacca
     3661 cccaactggc gaccggcggc caacaactga acgttcccgg catcagtggt ccggttgctg
     3721 cgtacagcgt cccggcaaac attggcgaac tgcccctgac gctgaccagc gaagtgaaca
     3781 aacaaaccag cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg accccatcag
     3841 ccttcttccc cagcagttat ttcacctacc aggaactagg cgtgatgagt gcagagcggc
     3901 tggaaggcgt tatgcgcctg acaccggcgt tggggcagca aaaactttat gttctggtct
     3961 ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct aaagcctatg
     4021 ccaaaggcgt cggtaactcg atcccggata ttcccgatcc ggttgctcgt cataccagcg
     4081 atggcttact gaaactgaaa gtcaaaacga actccagctc cagcgtgttg gtaggaccac
     4141 tatttggttc ttccgctcca gctccggtta cggtaggcaa cacggcggca ccagctgtgg
     4201 ctgcacctgc tccggtgccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt
     4261 attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac
     4321 tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt atcagcagtg
     4381 taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg gtgcgtctcc
     4441 tggcgcacct ttttttatgc ttccttcctg ggatatgagc gattttttat agtacttcac
     4501 ttcttcttca ctaagaatgt ccattatttc aatgcattat cagagattct tttcctttcg
     4561 ccggtagtgt ctggacgctc aggctacttt tccaggttat tttatttctg tattacagag
     4621 gttttatgat aggtcatatc acaaattctg gcggcaataa ctctttgatg aaacatgatg
     4681 tggtgcaagg aaataatata gtagatcttg atttactacg taatttcaat ggggtgacag
     4741 gtttaaacag agattacttt atttatatca gtgatatatt tttaaatata aaacaacgga
     4801 acgaaaaaaa tcatgcaata aatatgttac gtgaagtctc aattag
//
LOCUS       DAJTNV010000180         4829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNV010000180 DAJTNV010000000
VERSION     DAJTNV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..118
                     /locus_tag="OO199_003860"
                     /pseudo
     CDS             1..118
                     /locus_tag="OO199_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(168..1118)
                     /gene="uspE"
                     /locus_tag="OO199_003861"
     CDS             complement(168..1118)
                     /gene="uspE"
                     /locus_tag="OO199_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5537722.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1270..2022)
                     /gene="fnr"
                     /locus_tag="OO199_003862"
     CDS             complement(1270..2022)
                     /gene="fnr"
                     /locus_tag="OO199_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5537723.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2217..2732)
                     /gene="ogt"
                     /locus_tag="OO199_003863"
     CDS             complement(2217..2732)
                     /gene="ogt"
                     /locus_tag="OO199_003863"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5537724.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2743..2886)
                     /locus_tag="OO199_003864"
                     /pseudo
     CDS             complement(2743..2886)
                     /locus_tag="OO199_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3276..4091)
                     /locus_tag="OO199_003865"
     CDS             complement(3276..4091)
                     /locus_tag="OO199_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5537725.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4188..>4829
                     /locus_tag="OO199_003866"
     CDS             4188..>4829
                     /locus_tag="OO199_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5537726.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1280 a         1118 c         1145 g         1286 t
ORIGIN      
        1 gtggacgaca tcaatgtatg tgatttagtc atcgatgatt tttgtttata gcctggtctt
       61 tactgtgttg ctggcaatcc cgttgggaat ttatttcctt ggcggcgaag agcagtaagt
      121 aaaaaatagg cccgataact cgggccttgt cagttattga agagtcgtta atcgtcttct
      181 tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt taatgaccag caggtcgcag
      241 cgaagatgat caatcacctg ttccgccgtg ttgccgagga atgctgctga aataccggtg
      301 cgtccgaccg tgcccagaac cacaattccc gcctgtaaat gctccgccaa atcaggaatc
      361 acctcttcgg gcagaccttt ttctacgtgc gtcatgtttt cattaatgcc gaatttctgc
      421 cgcagggctt tcattgccag caaatgttgc ccacgaatgg catcgttata aacgctcggg
      481 tcaaattccg gcagttcaat cgcgatatta attggcgtta ccggataagc gccaaccaga
      541 tgaacttcgg tatggttgac ttgttctgcc agttcgatcg tctctttgac cagtttttca
      601 ttgagcgcat tatgatacgg ctcttcactg gcgagattca ccgccaccag cgccttgcct
      661 ccttccggcc acggctggtc tttcaccatc cacaccgggc ttgggcattt gcgtaacaga
      721 tgccagtccg ttggcgtaaa aatcaccgct tccagacggt catgttggtg cgccattttt
      781 agcaccaaat cgtgtccgcc gctgatcact tcctgaatga tggcttcgaa aggacggtta
      841 tgccagacca ctttaatttc aatgggaacg ccagcattga gataatattt tgcctgctcg
      901 tggatccagg ctgtacgctg gctgatgacg ccctgacgca tagtggtacg ttcgtccggg
      961 gagagcaggg tggtcatttc gtatgagaag tcatagatcg gcaaaaaggc tttaattttg
     1021 ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg ctggttggtc gtcctggtta
     1081 ggatcgataa caacgagcat gttctgatac atagccatac agggtctcct tacaacaact
     1141 gtcaacgcag tttgtaatta aaagattaac ccatatctgg tgaatgaaac agtgatgaac
     1201 cttctgccag atcaataaat cagaaaaatt taatgatatg acagaaggat agtgagttat
     1261 gcggaaaaat caggcaacgt tacgcgtatg accagcaagc tgggccagcg catcgttatt
     1321 ttcgatggtg atgtatttac ctttgactgc cagcataccg cttttctgga agcgacccag
     1381 cagacggctg atggtttcta ccgtcaggcc cagatagtta ccaatatcgc cacgagtcat
     1441 cgtcaggcgg aattcacgag gggagaagcc gcgttgggca aaacgacggg acaggttgta
     1501 gatgaatgca gccagacgtt cctcggcatt tttcttcgac aacagcaaga tcatgtcctg
     1561 atcgcctttg atttcaccgc tcatcagacg catcatctgc tgacgcagat tcggcatttt
     1621 accggacaaa tcgtccagcg tttcgaacgg aatttcacat accatcgagg tttccagcgc
     1681 ctgagcgaag ctcgggtgat ggccgctgcc gatggcgtca aatcccacta ggtcgcctgc
     1741 taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg attgtataac ttttaatcgt
     1801 accggagcgg atggcataaa gcgatttaag ttcatcacca gccttaaaca gcgtctggcc
     1861 tttctgaata ggcttcttcc gctcaatgat attatcaagc tgatcaagct catgttcgtt
     1921 gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc tggcaatgga tagcacaacc
     1981 gccagactga atgcgccgta taattcgctt ttccgggatc ataggtctgc tcaagccgta
     2041 attgatattt gtcaatttta acatcttttt agggagcaag taagtctaag caaaccttaa
     2101 cagcagagaa ttccgatatt agatgtaaat atatgtctat ctatttgaaa ctccttaagt
     2161 tgtcaagggt aactttacat aaaagtgtga acaagctggc acaaattgtt taatgtttac
     2221 agcaaaagat aaccttcgtg gcgcaataac cactcttttc gctgaactcc gcctgcatat
     2281 ccggtcatgg tgccgtttcg gccaataacc cgatggcaag gtacgacgat gctgatggga
     2341 ttcgatccgt ttgccgcacc aacggcacgc gccgcgccag ggcggcccaa ttgttcagcc
     2401 agttggccgt aatgcattac ctgcccgcag gggatagtgc gtagtgtttt ccagacttcg
     2461 cgctgaaatg gcgtcccccc cgtggcagtg ggaagcgtat caataatgct aagattaccg
     2521 gcaaaatatt cacgaagctt gtcgcttaaa ccacctgggt tggtggcaga aatgcgctca
     2581 tagccttctt tgcgataatg gatgtccagc agctgcacca tgcgttcgct gtactcttcc
     2641 cattcaaccg cccgcaggcg aaattgctca tcgcaaatca cccacagtgg acccagtggc
     2701 gtggcaattt tttcttcaag taatctcagc atccgttctc tcttaagaca aacgtgggta
     2761 aatacccgga cctatcggca gaccgacaag ataccacgcc agcaacatca gcagccatac
     2821 caccaaaaag ataagtggat agggcaagac taacgaatag taagtaccca gtttcgcgtc
     2881 tggtttgtcg cagtctgata gcataaagcg attttttaat ttttctgcct gaagaaatga
     2941 gggcaagcca tcacatggat cgagggtggg ataattccag tcagtgaaat tttaccgtag
     3001 gctatctgtt atttgattct gtggcgtgat agccgccaga caccactgaa ctcgtcactc
     3061 attccccaat cccggcgaga atgtggttat ttacagagct aaaaaatgaa taaaaaaata
     3121 gagcctgtcg acatccgcca gactctacag tacacacagc agtgcatcct cgtcttaaat
     3181 ccggcctggg gtcctggtga ccggtgacag tgttataacg gttttaccca tccgttgcca
     3241 attttcttca gtgacagatt tcacgaaaat ataagttaaa tcatttgtct tgtgattgca
     3301 taattatgca tcgttaatcg acgagtaaat ttaccgggcg agtagcccgt tcgacgctta
     3361 aacatattgc cgaacgattc agctgaggca tagcccagac gatgggcaat tttggtcaac
     3421 gaatatccat catcaagaag cgtcactgca gaaataacgt gcatttgctg aacccagtgg
     3481 cgaaatgtta atcccgtctc tttaacaaat aagcgcatga gtgtacggct actgacgtta
     3541 attttatccg ctagattatc cagtgttggc agataccctt ggttgtttag caaattctca
     3601 caaagctgct gtagccgctc atcatttggc caagggagtt caagtgacat tttcaccccc
     3661 atttttattt cctgaagtat cagtgtctcg agggctgaaa aacgtaactg ttgtttatct
     3721 gtggtacttt gcgacggtgg aagttggtta attgcaatta gtaattccct taacaggggt
     3781 gaaatacccg tcatgaagca aactgttccc agcgtttcac tttccgcttc tgaaaccagt
     3841 acggttttta aaagtacgtt gcctgtcgca acaaacttat gcggcatttg tgggggcagc
     3901 caaagcgcgc tgctggcatg gactagccag acgctttctc cagcccatac acgaacgact
     3961 ccttcagccg ggtaaagcaa ctgggcatag agatgtttat gccagggaac tgtacctcca
     4021 ttcagatgtt ccgaatccag tccgataatg tgatgccctg gtgatgcttt gggttgcagt
     4081 gtaattgtca tttacagtag gtattttgtc agaaatcatc aaataagcca tgttaaattt
     4141 taaatatgac gtatgcaagt ttccggatat atacggtgta aagagtgatg ataatttcta
     4201 aaaaacagct tattggggtg gtagcgatcg gtattctttt gactggcgtt gtctttttta
     4261 tctggagggt aagcaaagga agatttatac agacgacgga tgatgcttac attggtggta
     4321 atatcaccac ggtggcaagt aaagtttctg gttatatttc tgctatagag gtgcgcgata
     4381 atcagagcgt aaaaaaaggc gatattatcc tgcgtctcga tgatcgggat tatcgtgcaa
     4441 atgtcgccag gcttgaggca aaaataaaaa gtagcaaagc caatctcgaa ggcattcaag
     4501 ccactattac gatgcagcag tcgataattc aaagtgccag tgaaacatgg caggcagtaa
     4561 aacatgagga gcaaaagcgc ctgcgagata ctgaacgtta tgaaaaactc gcacaatcag
     4621 cggcaatatc gcaacaaatt attgataacg ccaggtttga ctaccagcag gtcgcagcaa
     4681 aggagcgcaa agccgcgaac gatttcctgg tggagaagca acggctggca gttttatctg
     4741 ctcaggaaga aaacgtacgt gcgagtatcg aagaagttca ggccgctttg actcaggcac
     4801 tactggatct ggaatatacc cttgttcac
//
LOCUS       DAJTNV010000181         4782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNV010000181 DAJTNV010000000
VERSION     DAJTNV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..347
                     /locus_tag="OO199_003867"
     CDS             <1..347
                     /locus_tag="OO199_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5537727.1"
                     /translation="NVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFA
                     NTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNEVSAGT
                     VQSVIGVTFIYE"
     gene            681..2960
                     /gene="ydeP"
                     /locus_tag="OO199_003868"
     CDS             681..2960
                     /gene="ydeP"
                     /locus_tag="OO199_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5537728.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3208..3411
                     /gene="safA"
                     /locus_tag="OO199_003869"
     CDS             3208..3411
                     /gene="safA"
                     /locus_tag="OO199_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5537729.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3479..4240
                     /gene="ydeO"
                     /locus_tag="OO199_003870"
     CDS             3479..4240
                     /gene="ydeO"
                     /locus_tag="OO199_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5537730.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4642..>4782
                     /locus_tag="OO199_003871"
     CDS             4642..>4782
                     /locus_tag="OO199_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5537731.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1366 a         1022 c         1063 g         1331 t
ORIGIN      
        1 gtaatgtcac cgtggatctc ccggactttc ctggaagcgc agaaataccc cttggcgtat
       61 attgctcaag cgaacaaaag ctctcatttt atctttcggg tgccaccact gacagtagcc
      121 gacaagtgtt tgctaacaca gcacctgatg caacaaaagc atcgggagtg ggtgtcactc
      181 taatgcgtaa tggaaaaata ctggctacgg gagaaaatgt ctccctgggg acagtaaata
      241 aaagcaaagt acctctgggt ttaagcgcaa cctatggaca aacaggaaac gaggtttcag
      301 ccggaaccgt ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgac
      361 cttagtctat gcgctccatt cacgaaaatt gcgcggataa ttattttgtg aaggctatta
      421 tcctacacct gtaagaaaat ccgcgcacaa ttgaagccag agtcttattt tttatattga
      481 aaaatatgtt aatttaaaca ctatgagaga tgatgcttaa cttatcgtat ttaatctatt
      541 gtttaacgat aatagattaa aaatagaaag agagtagtcc cttactctta tatatccatg
      601 ttggcgataa tccctttgta tgtactgtgc atcatcgcta ttacaaatcc taataattca
      661 tttccacaca ggataagtag atgaagaaaa aaattgaatc ctaccagggt gctgcaggtg
      721 gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccatg
      781 atgttattgc catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat
      841 ggccagatcc taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg
      901 cctgggaagt cacggataag caggtaaatg cctctttctt tgctgagaat acggttcaat
      961 cattacttac ctggggagat cacgagcttg aggctgcggg gcgtctcact cagcctttga
     1021 aatatgatgc cgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgacgaaa
     1081 ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc
     1141 gcacttccaa tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagcaata
     1201 actttcccga ctgctccaac atgtgccatg aaccgacaag cgtgggtttg gcagcgagta
     1261 tcggtgtagg taaagggacc gtgttgctgg aagactttga gaagtgcgat ttagtcattt
     1321 gcattgggca taaccctggt acaaaccacc ctcgcatgct gacttcgttg cgcgctttag
     1381 tgaaacgggg agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat
     1441 ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggccagtg
     1501 cctactacaa cgtgcgcatt ggtggcgata tggcgttgct caaggggatg atgcgcctgt
     1561 taattgagcg cgatgatgct gcaagtgccg caggtcggcc ctcattgctt gatgacgaat
     1621 ttattcaaac gcataccgtc ggctttgacg agctacgccg tgacgttctc aattccgagt
     1681 ggaaagatat cgaacgtatt tctggactaa gtcagacaca aatcgccgaa ctggctgacg
     1741 catatgccgc tgccgaacgc accattatct gttacggaat ggggatcact cagcacgaac
     1801 atggtaccca gaacgtgcag caactggtca atttgctgtt gatgaaaggt aacattggca
     1861 agcctggtgc gggtatctgc ccactacgtg gtcactctaa tgtacagggc gaccgaaccg
     1921 tcggtatcac cgagaaaccg tctgcagagt ttctggctcg cctgggtgag cgctatggct
     1981 tccccccacc tcatgcacct ggacatgctg caattgccag catgcaagca atatgtacgg
     2041 ggcaggctcg agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg
     2101 aagcgagcgc tgtaccgtta acgcaattag atttggcggt acacgtagcc actaagctta
     2161 accgctctca tctgctgacc gcacggcata gctatattct gccggttctg gggcgtagcg
     2221 agattgatat gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga
     2281 ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag
     2341 tggtcgcggg aatcgcgcag gcagcactac cccagagcgt ggtagcctgg gagtatctgg
     2401 tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgact
     2461 ataaccagcg catccgtcat cccggtggtt ttcacctgat aaatgcagct gctgaaaggc
     2521 gctggatgac gccgtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc
     2581 cctcttcagc gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca
     2641 acacgacgat ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg
     2701 tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc
     2761 ttattgcgct tacgccagac ggtaagcgca gctcacgccg catggataga ttaaaagtgg
     2821 tcatttaccc tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc
     2881 taacacttga taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg
     2941 aattagaacc atcaaattaa tgcctcttct catttcttct gctgtcatcc gcacagcaga
     3001 agaattcctc attgactatt attgcgcaat ttgctcacat ggattaaatt aaactacata
     3061 ctataagata taaacttctg cctacagctg taagaaactc cgctcagtac tgaagcacca
     3121 gtcctatttc ttcttttctc cagcctgtta tattaagcat actgattaac gacttttaac
     3181 gttatccgct aaataaacat atttgaaatg catgcgacca cagtgaaaaa caaaatcacg
     3241 caaagagata actataaaga aatcatgtct gcaattgtgg ttgtcttatt actgacactt
     3301 acgttgatag ccattttttc ggcaattgat cagctgagta tttcagaaat gggtcgcatt
     3361 gcaagagatc ttacacattt cattatcaat agtttgcaag actggaaata aacagcaaat
     3421 tataaatgtg aaatgtttaa aaggtatcga taaaacttta ttgttttaag gagataaaat
     3481 gtcgctcgtt tgttctgtta tatttattca tcatgctttc aacgctaaca ttttagataa
     3541 agattacgcc ttctccgacg gcgagatcct gatggtagat aacgctgttc gtacacattt
     3601 tgaaccttat gagcggcatt ttaaagaaat cggatttaat gaaaatacca ttaaaaaata
     3661 tctacaatgc actaacatcc agacagtgac ggtgcctgtt cctgcgaagt ttttacgtgc
     3721 ttcaaatgta ccgactggat tgcttaatga aatgattgct tatctcaact cggaagaacg
     3781 caatcatcat aatttttcag aacttttgct tttttcttgc ctgtctattt ttgccgcatg
     3841 caaaggtttc attacactat taactaacgg tgtgctatcc gtttccggga aagtgagaaa
     3901 tattgtcaac atgaagctgg cgcacccatg gaagctgaaa gatatttgtg actgcctgta
     3961 catcagtgaa agcctgttga agaaaaaact aaagcaagag caaacgacat tctcacagat
     4021 tctattagat gccagaatgc agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa
     4081 taaaattgcc gaacaatgtg gttatgccag tacatcttat tttatttatg cgttccgcaa
     4141 acatttcggc aacagtccga agagagtttc taaggagtac cgttgtcaaa ggcacacggg
     4201 tatgaatacg ggcaacacga tgagtgcttt agctatttga ttatttgcta acgagtagtc
     4261 aaccacgcac gctacgtaag aattatatgg ggcagccatt ccctgccccg cgttgttttt
     4321 aggcgatata tttattgaaa taaataagtg acatccatca catatttatg cacttgcata
     4381 acctgttgca tgattattta tgatctcaat tcggcatttt gtcagtaaaa tgcaataatt
     4441 tattaaatat caataaatta gttgtttatc ggcgagaaat tacttaatag aacagaaagt
     4501 aatgtcaacg ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttta
     4561 tgtgactaac ttcacttaca tccacttatt tctcttcgta aaattacttc ggaattaagt
     4621 acaataagaa gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg
     4681 atatctttga tactggcatc tggtatggct gcatttgctg ctcatgcggc agatgatgta
     4741 aagctgaaag caaccaaaac aaacgttgct ttctcagact tt
//
LOCUS       DAJTNV010000182         4735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNV010000182 DAJTNV010000000
VERSION     DAJTNV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.183"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..239
                     /locus_tag="OO199_003872"
     CDS             <1..239
                     /locus_tag="OO199_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5537732.1"
                     /translation="LSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQA
                     QVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(440..1297)
                     /gene="sitD"
                     /locus_tag="OO199_003873"
     CDS             complement(440..1297)
                     /gene="sitD"
                     /locus_tag="OO199_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5537733.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="OO199_003874"
     CDS             complement(1294..2151)
                     /gene="sitC"
                     /locus_tag="OO199_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5537734.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="OO199_003875"
     CDS             complement(2148..2975)
                     /gene="sitB"
                     /locus_tag="OO199_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5537735.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="OO199_003876"
     CDS             complement(2975..3889)
                     /gene="sitA"
                     /locus_tag="OO199_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5537736.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4362..>4735)
                     /locus_tag="OO199_003877"
     CDS             complement(4362..>4735)
                     /locus_tag="OO199_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5537737.1"
                     /translation="KLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYS
                     VSSLGTRWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEI
                     YGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1274 a         1146 c         1144 g         1171 t
ORIGIN      
        1 gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat atggcgtgga
       61 gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa ctggcggcag
      121 caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag cgggagctga
      181 aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc gtggaataac
      241 agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa ctttcgatag
      301 tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc atgatagaat
      361 tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct ggcggacgat
      421 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gcgtttccct
      481 ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc gcgaataaga
      541 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca ccagtcattg
      601 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
      661 gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata cctactgctt
      721 tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc agccatctcg
      781 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
      841 attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg attatatcgc
      901 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttaa
      961 ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccataactg
     1021 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg cagaatactc
     1081 cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc acgatgccag
     1141 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
     1201 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
     1261 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg
     1321 cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg ctaaaagaaa
     1381 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
     1441 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga gcaggcgggg
     1501 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
     1561 aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
     1621 gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt cgaaaaaggt
     1681 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
     1741 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
     1801 gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
     1861 gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
     1921 gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
     1981 tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc
     2041 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg ccgagaccca
     2101 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tcttgcttcc
     2161 ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt
     2221 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc
     2281 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
     2341 gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag catcgttttg
     2401 ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg
     2461 ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc tctcgccaga
     2521 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg aaaatccacc
     2581 atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt ggctatacgc
     2641 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
     2701 gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt
     2761 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
     2821 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
     2881 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
     2941 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccggctttaa
     3001 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta ggtaccgggc
     3061 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
     3121 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
     3181 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
     3241 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
     3301 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
     3361 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt tggtaggttt
     3421 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
     3481 cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt ccttcggtga
     3541 tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
     3601 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
     3661 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
     3721 ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc gcgatgatgg
     3781 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
     3841 tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata atttagtacc
     3901 tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca taacctatac
     3961 aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta ggttgtttcc
     4021 tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg aaatgacttt
     4081 atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg caaaaaaggg
     4141 gcaaaaaagg ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt
     4201 aacatattga aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac
     4261 ttgttcaggt cgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt
     4321 ctctggtact aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc
     4381 aacatggcta tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc
     4441 atttgcgcag tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc
     4501 aacagccagc aggcaaaagt atgtcgcgtg tggtacggat tacggcggcg aataccagca
     4561 cgttttactg ctgcattcca tcttgtaccc aaactgctta ccgagtaata aggtttctgt
     4621 ttttcgttac acaccctggg catgaaaaca aaatgcagtt tttgtttttc agttctgccg
     4681 tactcccgat gataaaaagt gatttcgctt ttgcgatgat gcccggtcag tttgt
//
LOCUS       DAJTNV010000183         4671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNV010000183 DAJTNV010000000
VERSION     DAJTNV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..528
                     /locus_tag="OO199_003878"
     CDS             <1..528
                     /locus_tag="OO199_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5537738.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            725..2164
                     /gene="aldA"
                     /locus_tag="OO199_003879"
     CDS             725..2164
                     /gene="aldA"
                     /locus_tag="OO199_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5537739.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2206..3207)
                     /gene="gap"
                     /locus_tag="OO199_003880"
     CDS             complement(2206..3207)
                     /gene="gap"
                     /locus_tag="OO199_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5537740.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3396..3926
                     /gene="cybB"
                     /locus_tag="OO199_003881"
     CDS             3396..3926
                     /gene="cybB"
                     /locus_tag="OO199_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5537741.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4171..4344
                     /locus_tag="OO199_003882"
     CDS             4171..4344
                     /locus_tag="OO199_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537742.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="OO199_003883"
     CDS             complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="OO199_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5537743.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1193 a         1078 c         1176 g         1224 t
ORIGIN      
        1 cgcaccactg gcagagcaga agcgaccatc ctggtggata tcgctcatca gttctggcat
       61 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      121 tttagcatca cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgtacag
      181 gaccccacca tgcagcggcg cacgatggca acgttccgcc gtatgaccgg ggacaatccc
      241 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      301 agtgtaatct ttattaatca gttacaagga ttatggccgg ttgagcgtta tctctcacta
      361 ctcactggcg agctgccgcg tttacacgat gatagcgatg gctacggtcc ccgcgggcga
      421 gattttatcg ttcacgttga ttttccggca gaagtcatcc atgcatggca aacgctgaaa
      481 catgatgcgg tgctcatcga ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt
      541 tgtgaaccac ttgtttgcaa acgggcatga ctcctgactt ttatttctgc cttttattcc
      601 ttttacactt gtttttatga agccctccac agacttgtcc tttcccgatg ccgtctctct
      661 gatgattgat gttaattaac aatatattca ccgaaaacaa acatataaat cacaagagtc
      721 gcccatgtca gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg
      781 tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt cccgcatacc
      841 cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga
      901 atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg tgcaaaatct ccgccgggat
      961 ccgcgaacgc gccagtgaaa tcagtgcgct gattgttgaa gaagggggca agatccagca
     1021 gctagctgaa gtcgaagtgg cttttactgc cgactatatc gattacatgg cggagtgggc
     1081 acggcgttac gagggcgaga ttattcaaag cgatcgtccg ggagaaaata ttcttctgtt
     1141 taaacgtgcg cttggtgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat
     1201 tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga
     1261 atttacgcca aacaatgcga ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg
     1321 cggcgtgttt aaccttgtac tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa
     1381 cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaga agatcatggc
     1441 gactgcggcg aaaaacatca ccaaagtgtg tctggaattg ggtggtaaag caccggctat
     1501 cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat
     1561 taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaag gcatttatga
     1621 tcagttcgtc aatctgctgg gtgaagcgtt gcaggcggtt caatttggta accccgctga
     1681 acgcaacgac attgcgatgg ggccgttgat taacgccgcg gcgctggaaa gggttgagca
     1741 aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga
     1801 ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat
     1861 tatgcatgag gaaacctttg gcccggtgct gccagttgtc gcatttgaca cgctggaaga
     1921 tgctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa
     1981 tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg
     2041 tgaaaacttc gaagctatgc aaggtttcca cgccggatgg cgtaaatccg gtattggcgg
     2101 cgcagatggt aaacatggcc tgcatgaata tctgcagacc caggtggttt atttacagtc
     2161 ttaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa
     2221 tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac
     2281 cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac
     2341 tgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcgg tataaccaaa
     2401 tgactcgtta ttggtggttg cttgtttaag tgcgttattc acctcttcgg cagtcacttt
     2461 ttttccgaga atggacacca gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc
     2521 atgacctttc agtttgccgc tcagttccgg gatcaccaga ccaatggctt ttgccgcccc
     2581 cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg
     2641 cgggccatcc accagtgact gggtgccagt ataggcatga atggtcgtca tcgtgccgac
     2701 ttctatcccg aaactgtcat gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca
     2761 tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg tcgtcattaa cgttataaac
     2821 gatggttttc atttcaccgg caggagcgga aatcaacacc ttcttcgcac cagcatcaag
     2881 atgcgcctgc gatttatcgg cggaggtata aaagccagta cattcgacaa tgatttctgc
     2941 ccctttcgct ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt
     3001 cccatcaacg ataagtgaat cttccgtaaa atcaacgctc caggggaacg gaccatagtt
     3061 tgaatcatgt ttcagcagat aggcgagaat ttttggggaa gtaagatcat taatagcgac
     3121 aacgtctatg ttgcttttga cttcaagtaa acgacgcaac accagtcgac cgatacgacc
     3181 aaaaccgtta ataccaactt tactcatggt tttctcctgt caggaacgtt cggatgaaaa
     3241 ttgatccttt ccaggcttag accaggattg tgggatgggc aatctccatt cttacagtga
     3301 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta
     3361 tgttaacgga aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac
     3421 aaatcagcat tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt
     3481 ttcgtggttt cttcccacgt agcgatcggc cgctcatcaa catgattcat gtttcctgtg
     3541 gcatctcaat tctcgtgctg atggtggttc gtcttttgtt aagactgaaa tacccaaccc
     3601 cgccgattat acctaagccg aaaccgatga tgacgggact ggcgcatttg ggacatttgg
     3661 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg
     3721 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
     3781 gggttgatag cttaaagtcg tggcatgaga cgctggcgaa tctgggatat tttgtcatcg
     3841 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
     3901 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     3961 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
     4021 atgacaaata aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc
     4081 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcgc
     4141 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg
     4201 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     4261 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
     4321 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
     4381 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     4441 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     4501 agtgtgaatg ccagaatcgt aatgcagata atgagcagac acaccaccag agggttgcgc
     4561 ttcatagcct ttctccttgc cggatggcgg gtaagaggct aacaatctga attgcttggt
     4621 tcattcgttg gcctcggttt gtactgaccc caaaaagttg gacagttaaa c
//
LOCUS       DAJTNV010000184         4642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNV010000184 DAJTNV010000000
VERSION     DAJTNV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..312
                     /locus_tag="OO199_003884"
     CDS             <1..312
                     /locus_tag="OO199_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5537744.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="OO199_003885"
     CDS             469..1218
                     /locus_tag="OO199_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5537745.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="OO199_003886"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="OO199_003886"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5537746.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OO199_003887"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OO199_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5537747.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..4018
                     /gene="yieH"
                     /locus_tag="OO199_003888"
     CDS             3362..4018
                     /gene="yieH"
                     /locus_tag="OO199_003888"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5537748.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4085..4558
                     /locus_tag="OO199_003889"
     CDS             4085..4558
                     /locus_tag="OO199_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5537749.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1241 a         1163 c         1139 g         1099 t
ORIGIN      
        1 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcatcagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata ttgtttccct
     3541 tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc
     3601 tcggctgttc gattcagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc
     3661 aatgtgtgtg gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa
     3721 tatgttgcac tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc
     3781 agacccggcg ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat
     3841 tctggttgat gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt
     3901 ctacttctgc gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac
     3961 ccatctttcg cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt
     4021 cttcacactc ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt
     4081 tgatatgtct gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc
     4141 gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct
     4201 tttggtaata atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat
     4261 tcctgcattg ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa
     4321 aaattatcgt tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc
     4381 agttattgta catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg
     4441 tgacagtctc gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat
     4501 cattacccgt ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc
     4561 gggatacttt atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat
     4621 caggccgtta ccccaattca aa
//
LOCUS       DAJTNV010000185         4625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNV010000185 DAJTNV010000000
VERSION     DAJTNV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.186"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            294..854
                     /locus_tag="OO199_003890"
     CDS             294..854
                     /locus_tag="OO199_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5537750.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            797..1186
                     /locus_tag="OO199_003891"
                     /pseudo
     CDS             797..1186
                     /locus_tag="OO199_003891"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1166..1723
                     /locus_tag="OO199_003892"
                     /pseudo
     CDS             1166..1723
                     /locus_tag="OO199_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1821..3430
                     /locus_tag="OO199_003893"
                     /pseudo
     CDS             1821..3430
                     /locus_tag="OO199_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3531..4022)
                     /locus_tag="OO199_003894"
     CDS             complement(3531..4022)
                     /locus_tag="OO199_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCR5537751.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4027..4203)
                     /locus_tag="OO199_003895"
     CDS             complement(4027..4203)
                     /locus_tag="OO199_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5537752.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1417 a          744 c          916 g         1548 t
ORIGIN      
        1 aatcccaatg cctttttcaa aataaattag gattaaaata attaaatcag taaattccga
       61 tgcatgattt cggatttttc gaaagtcctg atgaaaggct gcgctacggc actcacggat
      121 ttaattgtta ttcctaatgt atctatgaat taatgtttta taaggatttt ttataaccat
      181 ttataagtat tttcaagcct ggcttgttgc aaacaatgta taaagcactt aggcaataat
      241 aattacattc agcaactatc atcatcggta ttgtttgtgg gcggaatttc atattgacaa
      301 cagtacagtt cttatatcta ttaataatag aaagagatct acaacctaca gattggtgta
      361 gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc ttattgtttg
      421 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      481 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      541 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      601 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatta
      661 ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat tatttgtttc
      721 gtcagatgag tttgtgcata ttaatatttc tggcattgat gaataaagtt agtgaaaaca
      781 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg tttttgtttt
      841 tggggggccg atagttgctc acatactttc tagtcattat gagagctaca atttacacat
      901 tgccgagtta accaatgaaa atggtcaggt ggtttggaaa gcctcatatg ttaccataat
      961 gattttcatg tggttaacct tgctttcggt taatctatac tttaatggtt tacgctatga
     1021 tatctggaat ggagtgacgg tgattgcttt ttgtgccgta ctgtacaata tctctctgtt
     1081 atttatgagc aggtatagcg tgtccacttg gtatattagt cgcactattg aagtagtcag
     1141 taagttaacc gttatggtaa tatttatgtg ccatatattc agtgcgctac gagtaacaaa
     1201 gaacattgca catcgcgatc ccttaaccaa tatatttaac agaaattatt tttttaatga
     1261 actgacagtt caatcagcat cagccaaaaa aacgccttat tgtgtcatga ttatggatat
     1321 cgaccacttc aaagaagtca acgacacctg agggcatccg gttggcgatc aggtgataaa
     1381 aacagtggtg aatatcattg ggaaaagcat acgaccagat gaccttttag cgcgcgtcgg
     1441 cggcgaagag tttggcgtct tgctgacgga catcgatact gaacgtgcga aagctttagc
     1501 ggaaaggatt cgggaaaatg ttgagcgttt aactggcgat aatcctgaat acgctatacc
     1561 acaaaaagtg acgattagta ttggcgctgt tgtgactcag gaaaatgcgt taaacccgaa
     1621 tgagatctat cgactggctg ataacgcact atacgaggcg aaagagaccg ggcgtaataa
     1681 ggtggttgtg agggatgtgg tgagtttttg tgagtcacca taaagcggca ttttagcggg
     1741 tgattttggt aagcgctaaa tagctattcg cagacttgcg taacaatttt cagaaatatc
     1801 atagtactat atgatcttaa atgcttatca ttgatcaatc tgctatcacc cgcttttact
     1861 ggcaattgta ctataatttc ttaggttgca tcaccctgat gcgtggggaa acatgtatgg
     1921 tattcaacaa aaaaatgttt gtactcatta taataccagg cattctgggg gtgttactat
     1981 cgtttgctat gtcaatattt caaatgaata gagatacgac aattaccgcc ggtattctgt
     2041 taaaacagct tgataatgtt acgcaaattg tccagcatac gacaaagctt acctctattc
     2101 tggtgatgaa gccctgcaaa gacattcttg aacagttgat tgctaacgga gcattgactc
     2161 cctatgttcg aacaacgggt cttatagaaa ataatttcca aatatgttct tcagttagcg
     2221 gtttcaaaaa aatgaatgtt aacgatgttt acggtacgag ctttcacaat aaaaataaag
     2281 aatcacgtat agtatcaatt agcggtacaa gttttgtgcc aggtaaaaca gcaatagtgt
     2341 tccttatgcc aatagggaat gatatgacag cttttagtat tgtagagtca cgatatatct
     2401 atgatctaat ggatgttctt gatgatgaga atgatgattc attttctctt cgttttactg
     2461 aagggcctgc aattattagc ggtgtaaata ataatgacag gttatatatg ttaaaaagag
     2521 attttaattc tgcgatcagt caggctcgtc ttacagttac tacgccgatg atatcgttat
     2581 atccctatat cattagcaat gtgctttata ttttgccgtt atctattatt ctctctttta
     2641 ttctctattt cttatggcag cactggatgt cacggaaaat gtcgctggca gaagaaatta
     2701 aaaaaggaat gtcatccgga gaattttctg tgcattatca accagtgtgc gacacgacaa
     2761 ccaaagcgtg tctgggggtt gaagcgttaa tgcgctggca acgcgaagat ggtaaaaata
     2821 tatctccggt agtttttatc cgcgctgcag aagaggaaaa tttaataata ccgttaacta
     2881 aacatctctt tgaactgatt attcaggatg tacaaagctg gaaagtgaaa aagccgtttc
     2941 accttgggat aaatattgcc gcggaacatc ttgcacatcc tgactttgtt ggtgatgttt
     3001 tacatattaa aaatgcaata tctgataaat ttaatattgt ccttgagatt accgaacgca
     3061 atctggttga agacactgat cttgcattac agaaaataaa tgaattaagg atccatggct
     3121 gtgaatttgc agtcgatgat tttggcacgg gatattgttc ccttggcctt ctgcaaaagt
     3181 tgtctgttga ttatcttaaa atagataaaa gctttataga cacacttact acagcggaag
     3241 acgaaacgcc agttttagat attattatta agctgagtaa tcggctcaat ttgataacga
     3301 ttgcagaagg tgtcagtact cctcaccagc agaatatctt attcataata acgtgacatt
     3361 agttcaggga tacttatatg aaaaaccaat gaaagcgaca gagttttatc agtggtatgt
     3421 aaacagataa ataattaaaa atattggaat ggttttgtat tgtcttaaaa tataaacaaa
     3481 gcaatcagaa tgttattaac tgatgacaaa cgccaagatc aacaatgctg ttagtgagta
     3541 atacatatga atcgtggtgt cctgcctgta gagaatatgt agttataatc attttgacgc
     3601 gtaacataat aattaattga agtgtctgct tttatgaaga aatcagaagt gatattttca
     3661 aggatttcat cgctgatgtt atcatctttt tgtatttttt ggattttaac tgtggtgaga
     3721 taaacggtat cacggaattt cttgtatgta aaactcacat atcggcggat gaagcccatt
     3781 ttttttccat ctaccgtgta tgcgcccgtg agatacatgt ttcctgtgtt attttcagaa
     3841 atcatattta acgaaaaata ttgatccatt tttgtatgat cggtgaaatt aataacatgg
     3901 gcattacatg atatgttttg ctctgctggt tcattggaat gatgaaacgc aatatggctc
     3961 agaattgctg caataaaagc acataaaatt attcccgaaa acagaaaata tctttgagtc
     4021 atatgattac tccgctttat aatagagaga gttgcaatta tttggtgatt cagagtttaa
     4081 catttgacta caggtgaaag cataaaaccc gctcatgcta tcatttgttg caatgtaaat
     4141 gtaaggatgt attttgcagt tgacttccat tttctgcaat ttttcaataa ttggtgatag
     4201 catgtctacg gaattaaagt aattattatc atagaaataa ctgcatcctt cctgagtagg
     4261 gtgttgagca taatttgaga aagcatctat tttttttata gtgtgagatt tgctttgaaa
     4321 agccatatcg aaaaaataag aagacatata aactatcccc catgccgcaa aaaaaaacta
     4381 taagtaatgt attctttgtg gtaaatatac cttttgcaac gtaattttta tttatatttt
     4441 cagtgtgggt gccattctct tcactctcaa cactttcatt ctcattgtga ttattgtcga
     4501 gaacagttat gttgatttct tcattgattt tgaagccttt ccgtggcacc gttctgataa
     4561 ctgatcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     4621 ccgga
//
LOCUS       DAJTNV010000186         4610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNV010000186 DAJTNV010000000
VERSION     DAJTNV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="OO199_003896"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="OO199_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="OO199_003897"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="OO199_003897"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR5537753.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4610
                     /gene="pgaB"
                     /locus_tag="OO199_003898"
                     /pseudo
     CDS             4153..4610
                     /gene="pgaB"
                     /locus_tag="OO199_003898"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1317 a         1033 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa
//
LOCUS       DAJTNV010000187         4560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNV010000187 DAJTNV010000000
VERSION     DAJTNV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..1836)
                     /gene="cheA"
                     /locus_tag="OO199_003899"
     CDS             complement(<1..1836)
                     /gene="cheA"
                     /locus_tag="OO199_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5537754.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLES"
     gene            complement(1841..2767)
                     /gene="motB"
                     /locus_tag="OO199_003900"
     CDS             complement(1841..2767)
                     /gene="motB"
                     /locus_tag="OO199_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5537755.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(2764..3651)
                     /gene="motA"
                     /locus_tag="OO199_003901"
     CDS             complement(2764..3651)
                     /gene="motA"
                     /locus_tag="OO199_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5537756.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(3778..4356)
                     /gene="flhC"
                     /locus_tag="OO199_003902"
     CDS             complement(3778..4356)
                     /gene="flhC"
                     /locus_tag="OO199_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5537757.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(4359..>4560)
                     /locus_tag="OO199_003903"
     CDS             complement(4359..>4560)
                     /locus_tag="OO199_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5537758.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1038 a         1241 c         1132 g         1149 t
ORIGIN      
        1 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
       61 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc
      121 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
      181 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
      241 cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
      301 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga
      361 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
      421 gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag
      481 cattgcaact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
      541 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
      601 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
      661 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
      721 gtcgataatg cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag
      781 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
      841 gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
      901 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct
      961 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
     1021 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
     1081 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc
     1141 tatttttggc gaaacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
     1201 gagtaccgct gtgatgtcat cttcggcgat atcgcccggt aatattgccg agagagaatc
     1261 cgcccctttc accacgtcat ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
     1321 gacttcgccg gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactttg
     1381 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
     1441 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcgt agctggcggc
     1501 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     1561 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     1621 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     1681 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     1741 cggctgcaaa accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa
     1801 aaatgtctga taaaaatcgc ttatatcgat gctcacgctg tcacctcggt tcggctgatg
     1861 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttctagggcg cttactggct
     1921 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     1981 acaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     2041 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     2101 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc
     2161 tggcgtaggg aaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
     2221 gcacaggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     2281 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     2341 gatcgatttt gagatggaga cgtaacgccc gcagtttcgg atcggactct atcaactggt
     2401 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
     2461 tgttcggctg cttattcact tccccctggc tctgggtgta atcatcacca ccgccgggaa
     2521 ttgggctttc actattggaa atgcgatcgc cgcccgtaac cgctgtcgcc agtggagtcc
     2581 ggaagtactc cgcaatctga atcaactctt ttgggctgga gatggagatc agccacatca
     2641 ccaaaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
     2701 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
     2761 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
     2821 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
     2881 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
     2941 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctagtgggg aaataaatcc
     3001 gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcacccccag
     3061 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac
     3121 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
     3181 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
     3241 gtgaccgctg ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc
     3301 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
     3361 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
     3421 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgctttcag
     3481 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
     3541 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
     3601 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
     3661 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa
     3721 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta
     3781 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
     3841 aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc
     3901 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
     3961 cagtaatcca ctttcaacaa accgcaccaa tgtcctggca cgggttaatg ccagcagtgg
     4021 tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
     4081 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc
     4141 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
     4201 cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt
     4261 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
     4321 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
     4381 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
     4441 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg
     4501 tcaaaacgga agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaaga
//
LOCUS       DAJTNV010000188         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNV010000188 DAJTNV010000000
VERSION     DAJTNV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1224
                     /locus_tag="OO199_003904"
     CDS             <1..1224
                     /locus_tag="OO199_003904"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5537759.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="OO199_003905"
     CDS             1224..1898
                     /locus_tag="OO199_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5537760.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="OO199_003906"
     CDS             2426..3187
                     /locus_tag="OO199_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5537761.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OO199_003907"
     CDS             3231..4310
                     /locus_tag="OO199_003907"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5537762.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="OO199_003908"
     CDS             4307..>4550
                     /locus_tag="OO199_003908"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5537763.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAJTNV010000189         4531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNV010000189 DAJTNV010000000
VERSION     DAJTNV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.190"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..253
                     /locus_tag="OO199_003909"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO199_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1489
                     /locus_tag="OO199_003910"
     CDS             746..1489
                     /locus_tag="OO199_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5537764.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVM
                     DRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1504..1926
                     /locus_tag="OO199_003911"
     CDS             1504..1926
                     /locus_tag="OO199_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5537765.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1984..2340
                     /locus_tag="OO199_003912"
     CDS             1984..2340
                     /locus_tag="OO199_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537766.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2433..2651
                     /locus_tag="OO199_003913"
     CDS             2433..2651
                     /locus_tag="OO199_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5537767.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2653..3018
                     /locus_tag="OO199_003914"
     CDS             2653..3018
                     /locus_tag="OO199_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5537768.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            3015..3680
                     /locus_tag="OO199_003915"
     CDS             3015..3680
                     /locus_tag="OO199_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537769.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3680..4045
                     /locus_tag="OO199_003916"
     CDS             3680..4045
                     /locus_tag="OO199_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5537770.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1213 a          967 c         1278 g         1073 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
     1381 tctcggcgag cgggtgatgg atcgcatggt catgaacggc gggcgctggg ttaattttaa
     1441 ctgggagagc tggcgtccga atgttagcca ttcgagggtt gttaagtagt ttcaggagga
     1501 tttatggcga aaccttttac tcccgaacag cgggaagaac tgaagacgcg aattgtggaa
     1561 ctcgtgcatc aggacggtcg ggtcacgatt cggcagttgt ccgatgaaac aggtatcagt
     1621 cgtgcgtctg tcggtcgctt atgcatagaa ctggtcgcaa gtggtgatgt atataattct
     1681 ggctacggct tattcccgtc tgaacaggct cgcaaggact ggcaaagcgc ccgcaaaaaa
     1741 ctctcgagag taaaggtgag gaaaccggtt gttgttgatc cggaccttat ctggtcatta
     1801 cctgacggag aaatacgccg ctacgacagg cgcctgaata taatctgtcg cgagtgccgg
     1861 aagagcgaag ctatgcagcg tgtactggct ttctatcagg gtaattttca ggaggcgata
     1921 ctgtgaatga aattagctat caggcttcaa ttaccgctgg cattcgcatc aaaggagagg
     1981 agcatggaaa taaaaccaga agatgagtta agcaatatcg ttttatttcc gataaaagag
     2041 gatgaccctc gtaatcaggt taattttctt tataagccat cggaaagacc atattgccat
     2101 cacgcctctg tacgggttga cgaaaaagag cgtcaggtcc gctgtaaaat ctgcggtgcg
     2161 gttgtggagc cgtttgactg gatgctctct gtggcaaaaa gagaaaccag actggcagat
     2221 gatgtaaggc tattgcgcca ggaggaacag gaaaggcgga gaaatataga aaagctgata
     2281 cagattgagc gtaacgcgaa agcgcggata cgcagggcga caaaatccag aactgaataa
     2341 ttaaatttag cactgttaaa aatttaatcc ttaaccggag ggatttctgc accctcaaat
     2401 catcaggaga ccgcccgaaa gggcggtaat gaatggttac attatttaga aaaaaatatc
     2461 cgcgaaagag tagaacaaca gaattcctgt ttctcattct gtttatcgtg ttgatgacac
     2521 cgatatcccc gcaaattttt gtctggataa tcggaaaaat aattgagcca gttattgaat
     2581 tgtataacga cgtggtatgg gcgtcattca acacactgca caataaaatt aatccgtata
     2641 aggaaagctg atatggcact gacgaaaaaa caacgtgcag aactgcgcat gaagttcggt
     2701 ggtcgctgcg cttattgcgg ctgcgaactt ggcgaaaagt ggcatgcaga ccatgtaaaa
     2761 ccggtcattc gttttgatgg aaatatgctt caccaggaac gtgacgatat atccaacatg
     2821 gtaccagaat gccacccatg caatctgcac aagcattgca gtagcctgga agattatcga
     2881 cgaattatca gtgatggtcg tcgtgaattc cttgcgtccg ggaaaggcaa agcgctggtt
     2941 cgtatgggat tggttgaaat gaaatctgac ccggttgtgt tctggtttga aaaatatcaa
     3001 gaaggggcta cggcatgact actattacca aagagcgact gcaatggctg gctaacattt
     3061 ctggccgcga tgatattgac gatatagacg gcggtgaaat tcgtgaactg gcgcttatcg
     3121 ctctggcatc actggaagca gagcctgtag cggaatgcat tgttgaagat gggggcatgt
     3181 gtattgacgg gttcggtgag tatgtgggtc actcgctgcc tgatggaatg catgagcttt
     3241 atgctgcccc gcgaatgcga caacctgatg gatggaaagc cgtagctgta gcgtggaagg
     3301 tgacgtttac tcaagttgac caggaatcta atacgttcac tgctatatat ttttacaaag
     3361 cggaagttga gcgttgggta cgactgcata aagcatgtga ttttcgggca gatataacac
     3421 cgctttacgc agcgccggca gtgccggttg cagtaaacga cgacatggct tacgcattcc
     3481 atcacgcact gtcagattca tcgcttggct ctgatgaaat cgaagaaatt aaaaccggtt
     3541 tgcgtgctgc ctttgccaac gtcactatcc aaccagagcc gatagtgccg gatgaaatcg
     3601 ggccaaacga tagtaatacg tttgattatg ttgatggctg gaacgcctgc cgcgctgcca
     3661 tgcttaaggg agataaataa tgattaatcg aaccaaactg gagcacattc tcgaatatgc
     3721 caggcagcag aaatgtattg ggcaactttg taaaattcca ccaggagata tggttgaaat
     3781 cgtggaaatg gccatgcgta aggctggcaa ctctccggta actctggctg gctggataag
     3841 ctgtagtgat gcagttcctg ctgaatattg cgatgtgatt cttctcgatg atctcgggaa
     3901 tgtattcccc ggttcctggg ataaggtttt ttgccccact cgtggcggga ataagatggc
     3961 ttttgtggac aaagacggcg ttgaagtaga gagctcaact cactggatgc cgctaccgga
     4021 accaccgcag gaggtgaatc aatgacctgg cctgaggcat tcaccacggt aggaattgcg
     4081 atggcggtgg cgctggtggt gtattcgatt tgccgttggg gttaacaaac aaaaaccccg
     4141 gattgatggt ccggggtttt tgaaggaaac aaaacagaaa cagcaattgc cgttacctgt
     4201 tgttaccatg gcaagtaaac gtatctcagg cgagcgcatt gcgccgttct gacgcagata
     4261 aattagcctg gatagaaggt gctggcaata aaaaatagcg ttttcttatc ggtatcggta
     4321 agattgctgc gggtgcttga ggctgtctgc ctcgggcatg ccactgtaag gcagacagag
     4381 aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct gaggcccaat
     4441 ctatgtctca caaatgtagg ttagcctctt acgtgccgaa aggcaaggag aagcaggcta
     4501 tgaagcagca aaaggcgatg ttaatcgccc t
//
LOCUS       DAJTNV010000190         4391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNV010000190 DAJTNV010000000
VERSION     DAJTNV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.191"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..268
                     /locus_tag="OO199_003917"
     CDS             <1..268
                     /locus_tag="OO199_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537771.1"
                     /translation="APFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQLTFS
                     GSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            268..723
                     /locus_tag="OO199_003918"
     CDS             268..723
                     /locus_tag="OO199_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5537772.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            726..1334
                     /locus_tag="OO199_003919"
     CDS             726..1334
                     /locus_tag="OO199_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5537773.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            1344..2675
                     /locus_tag="OO199_003920"
     CDS             1344..2675
                     /locus_tag="OO199_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5537774.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            2676..>4391
                     /locus_tag="OO199_003921"
     CDS             2676..>4391
                     /locus_tag="OO199_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5537775.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKR"
BASE COUNT         1201 a         1080 c         1156 g          954 t
ORIGIN      
        1 ggcaccattc ttcgacaccg ttaacaacaa gatggttgtg ttcaacgaga acaaaaccct
       61 gctcttcaag ttgtcgattg tcggcacgtg gcccagcgga accgccaaca ggtcaatgca
      121 gctaaccttt tccggctctg ttcctgacac actggtcagc agtcgtaatg cggcgacaac
      181 aaccgacaac atcctgttag ctacgttctt cagcgtggat aaagacggct ttcttgccac
      241 aaccatcaag ataatcgcgg agcagtgatg attacattca aaccaacgcg aaacatcgac
      301 ctgatagaag cagtcggaaa tcaccctgac attatcgccg ggagcaacaa cggtgatgga
      361 tacgactaca aacctgaatg ccgttacttt gaggtgaacg tgcacggtca gtttggcggc
      421 attgtttact atcaggagat tcagccgctg acattagatt gccacgccat gtacctgcca
      481 gagattcgcg gcttcagcaa ggaaattggg ctggcgttct ggcgatacat tctgactaac
      541 accaccgttc agtgcgtaac atcgttcgct gcacgcaaat tccgacacgg tcagatgtac
      601 tgcgcaatga ttggccttaa gcgtgtagga accatcaaga aatacttcaa aggcgtggat
      661 gacgtgactt tttacagcgc cacacgcgaa gaactaatcg acttcctgaa tcacgggaga
      721 tagccatgtt atatgcattt aagctgggca gaaaactgtg cggcgaggaa ccttattgcc
      781 ctgaaaaagg cgggaaaggt ggcagttctg ataaaagcgc aaagtatgca gcagaagccc
      841 agaagtatgc tgcagacctg caaaatcagc agtggcagac gatcatgaaa aaccttgctc
      901 cgttcacgcc tcttgcggag cagtatgtta accagcttca gaacctttcc agtttagaag
      961 gtcaggggca ggcacttaat cagtattaca actctaagca gtataaagac cttgcagggc
     1021 aggcgcgtta ccagagtctt gctgctgcgg aggcgacggg tggacttggt tcgacagcca
     1081 caagcaatca actggctacg atcgcgccga cactcggtca gtcttggtta tcaaatcaga
     1141 tgagcaatta caacaatctg gcaaacgttg ggcttggtgc gctgcaaggt caggcaaacg
     1201 ccgggcagac gtacgccaac aacatgagca gcattgcaca gcaaagcgca gctcttgcca
     1261 gccttttggg aacatcaacg ccttggggcg ctggtattgg tgctggtatc ggattgcttg
     1321 gctcgttgtt ttaaggggta atcatggcta cttggcaagg aacaaacggc ggattgttgg
     1381 ctggtatcgg cggcgtcaac tcaaacgctc cgagcgtaaa tgacatcggc aatacgcttc
     1441 agcttatcag gcagaacaat gatattgagc gttcaggcgc taacaatgtt gggctgactg
     1501 ctttgcaagg tctttcaggt attgcgggtg tttttcagca ggaaaagcag gctcagcggc
     1561 agaaagaatt tcagcaggcg tacgctaatg cttatgcgtc tggtgatcgc ggtgctttgc
     1621 gtcagttggc tactcaatat ccagaccaga ttgaatccgt tcgtaaaggc atgggattca
     1681 ttgatgaaga gcagcgtaat tctatcggca ccttagcggc tggcgcacgc cttgcgtcat
     1741 cgtctccaga agcaatgcaa tcatggctgc aaaacaacgc cggtgagtta gctcgtgttg
     1801 gcgttaatcc tcatgacgtc gctcagatgt accaacagaa cccgcggcag ttcggcgaat
     1861 ttgtcgatca cctggggatg aacagtctcg ggcccgaaaa atactttgac ctacaggata
     1921 aaatgcaggg tcgccaggtt accatgcgcg gtcaggatct ggattcgcaa accgccgctc
     1981 ggaatcaggc aatcacaatg cgcggacaag atatccaggc gaatttaggt cagcagcgca
     2041 ttaatctgga cgcagaaaca aaccgcatta acaacgaaaa taagcgcctt gaccggatgc
     2101 tatcagcaga aactaacgac ctgaagcgcc aggaaataca gagccgcata gcagccaaca
     2161 accagcagtt gcagcagaag cagcaagcgc taaatgatgg ctacaaagac ggcatcaaca
     2221 ccctcacaac cagcatgttc actctgaacg atatcgttag ttctccttca cttaagagca
     2281 ttacaggctt acgtggagta atccccaacg ttccaggctc acaggctgca gacactcagg
     2341 cacgacttga tacctttaaa tcacaagcat acctgacagc ggttcaggcc atgcgaggca
     2401 tgggcgcact ttctgatgcc gagggcaaaa agctcgacca ggctgttggt tcgctgcaga
     2461 actcgcagag cgaggagtcc tttcgtcgca acgctggcgt catcctgaac acgctcaacc
     2521 agaagcgtaa tgaggcggtt ggtaagtacg ttcagcaaaa cggtatcaag cgagtggaag
     2581 cgcctcaggc ttctatagat tacctgaagc agcaccccga gctgtcaatc gacttcatta
     2641 atcgctacgg atatcttcca tctttggggc agtaaatggc taattaccgt gatttgttag
     2701 agcaggctgg cgcacgttac ggtgtgccag aagggttgat gactgcactg ggtgccaagg
     2761 agtcttctta caaccctgcc gcagtaagct ccgccggggc tgtaggattg actcaggtca
     2821 tgcctgggac atggcgtgat atgggttata ccgatgagca aatgcaaaac cccgaatatc
     2881 aggctgacgc tggcgcgcgc tatctggcaa agatgtacca gcagtttggt aactggcgtg
     2941 acgctcttca ggcttatcac gacggtcccg gcaacgttat gaaggcaaag cgtggtgaat
     3001 atacgccagg acctgaaggc cgcggttacg ttgatgatcg ctttgctcaa tgggctggtg
     3061 acccggtgac agactcaaca gtcgaacagc gcgccacctc tgcaaaggta catcctcagc
     3121 aagaccctaa caacccgttt gcacaactgg aagtacagtc atccgaacaa gtatcggcat
     3181 caggtgtgca gtcagaccca aataatccat ttgctcagat tgagcagcag gcagccagtc
     3241 agcagccacc tcaacccgta agttctgtcg caccgaaacc tgttcagcaa caaacagtta
     3301 atcaggccaa taatgcacca acacgggaag aaccatcatt gatgcaacaa gctggcgatt
     3361 ggctcactgg tggtcaaagt gcagggcaaa ttgcagaaca ggctggtcgt ggtctggtaa
     3421 acataccatt tgacgtattg cagggtggcg caagtctgat taatgcaatc agtcaggggc
     3481 ttggggggcc aaaagtattg gatgatgttt atcgtccagt agacagaccg acagacccct
     3541 acgcgcaagc tggagagtca ataggcggtt atcttgttcc tggagcaggt gtagctggaa
     3601 acatggtcat tggttctctc gctgacgcgg cgaatcaacg gggtgatttt gccgaaaatg
     3661 ccgctattaa tgccggactt agcattgcta cgcatggcct gataaatggc gttacccgtg
     3721 gtgttcgtgg tgcatcaaat ataattagtg gcaataaaac atctgcacag agagcgacca
     3781 ctgcgccaac agaaacatca ccattctccg gtgatgccgc tgcagcaaca aatcctgcgg
     3841 ttcatgccgc agaggcaaga gtagcacaag gtgtaccaat gacgcctgcg acgaagaacc
     3901 cagaggaagt cgttcgcaca gtagcagcac aaaaaaggcc aaatctcgct tcatcgcttg
     3961 atgaactaga tatcaatcct caggctgaag ttctggagtc tgctgaaagg cttaatgttg
     4021 attcattact cccttcacac ttttccggga acgagcaata caaggcagtt gagcaagcaa
     4081 tcaagtcccg tgcgggttct gctctacagg tgcaggaaaa tgaagcaatc aggcagctag
     4141 cacagggcgc gggtgagata attgatcgcg tttccggtgc aaaagatgct cttggtatga
     4201 gcgacaagtt tattgatacg gtcaatggaa gaatgtctgc gctgatgaaa cgaagcgacc
     4261 agctttatcg caatgttgaa aaggcgatgc ctgcaggtgc aaaaattgat gcgccatcaa
     4321 caaggtcaat gctcaaacag gtggcagaag atcttggcgg gatgaaaaac cttgacccta
     4381 ttgaaaagag a
//
LOCUS       DAJTNV010000191         4328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNV010000191 DAJTNV010000000
VERSION     DAJTNV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..904)
                     /locus_tag="OO199_003922"
     CDS             complement(<1..904)
                     /locus_tag="OO199_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5537776.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO199_003923"
     CDS             964..1242
                     /locus_tag="OO199_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537777.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO199_003924"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO199_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5537778.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO199_003925"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO199_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5537779.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO199_003926"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO199_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5537780.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO199_003927"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO199_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5537781.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..>4328
                     /locus_tag="OO199_003928"
     CDS             4234..>4328
                     /locus_tag="OO199_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR5537782.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1274 a          924 c          926 g         1204 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggtt
//
LOCUS       DAJTNV010000192         4232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNV010000192 DAJTNV010000000
VERSION     DAJTNV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            19..900
                     /gene="yddG"
                     /locus_tag="OO199_003929"
     CDS             19..900
                     /gene="yddG"
                     /locus_tag="OO199_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5537783.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1155..2255
                     /gene="ompC"
                     /locus_tag="OO199_003930"
     CDS             1155..2255
                     /gene="ompC"
                     /locus_tag="OO199_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5537784.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2419..2904
                     /locus_tag="OO199_003931"
     CDS             2419..2904
                     /locus_tag="OO199_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5537785.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3014..4003
                     /locus_tag="OO199_003932"
     CDS             3014..4003
                     /locus_tag="OO199_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5537786.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1312 a          873 c          802 g         1245 t
ORIGIN      
        1 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
       61 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggcccggtc
      121 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
      181 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
      241 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
      301 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
      361 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
      421 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
      481 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
      541 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
      601 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
      661 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac tgcatggaat
      721 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
      781 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
      841 ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
      901 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
      961 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     1021 aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt aaaaacagta
     1081 tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa
     1141 aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg
     1201 cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg
     1261 gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt
     1321 atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc
     1381 actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga
     1441 cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta
     1501 actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg
     1561 atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc
     1621 gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca
     1681 aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg
     1741 acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata
     1801 ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc aatgcctccg
     1861 gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg
     1921 cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca
     1981 aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac
     2041 tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag gacatgattg
     2101 agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt
     2161 ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata
     2221 acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc
     2281 tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaattct
     2341 tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac
     2401 tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt aaaaaataat
     2461 gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag caatttttgt
     2521 gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag agcaattgcc
     2581 gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt gtcatatctt
     2641 gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct ggaaaacaat
     2701 tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa cctcagcgtt
     2761 aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat ttatattgat
     2821 cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa tctggaagcg
     2881 caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat ttcttaatct
     2941 tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga ttgagttaag
     3001 tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga tgaaaacaat
     3061 cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa aactggaata
     3121 tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac taatatcatc
     3181 aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg cacaaatgca
     3241 gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca ttgatttatc
     3301 aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta tcaatcttga
     3361 taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa agctaagtct
     3421 gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa gtattacaca
     3481 tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt catcagttac
     3541 ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt gcgaatggct
     3601 gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta ataaaaatga
     3661 actcttactg ctggatctat cccataataa actaacaagt ttacacaatg tcttatttcc
     3721 caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa tattctatag
     3781 caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat atataaatct
     3841 tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata ataaaataac
     3901 ccacattgat actaataata catccgatat tggaacttta ttccccataa taaaacagag
     3961 caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta tgcagctcat
     4021 gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa ttctttcgcc
     4081 tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt ttcactatac
     4141 tattaaaaaa gcatataccc catataatat acacacttac aagacagagg aagttgtaaa
     4201 ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTNV010000193         4197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNV010000193 DAJTNV010000000
VERSION     DAJTNV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..157)
                     /locus_tag="OO199_003933"
     CDS             complement(<1..157)
                     /locus_tag="OO199_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5537787.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="OO199_003934"
     CDS             complement(307..468)
                     /locus_tag="OO199_003934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537788.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="OO199_003935"
     CDS             complement(465..653)
                     /locus_tag="OO199_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5537789.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="OO199_003936"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="OO199_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5537790.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="OO199_003937"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="OO199_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="OO199_003938"
     tRNA            complement(2160..2236)
                     /locus_tag="OO199_003938"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="OO199_003939"
     tRNA            complement(2338..2413)
                     /locus_tag="OO199_003939"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="OO199_003940"
     CDS             complement(2558..3379)
                     /locus_tag="OO199_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5537791.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="OO199_003941"
     CDS             complement(3394..3750)
                     /locus_tag="OO199_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5537792.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4197)
                     /locus_tag="OO199_003942"
     CDS             complement(3763..>4197)
                     /locus_tag="OO199_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5537793.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1160 a         1136 c          826 g         1075 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccgg
//
LOCUS       DAJTNV010000194         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNV010000194 DAJTNV010000000
VERSION     DAJTNV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO199_003943"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO199_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5537794.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO199_003944"
     tRNA            complement(324..399)
                     /locus_tag="OO199_003944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO199_003945"
     tRNA            complement(406..480)
                     /locus_tag="OO199_003945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO199_003946"
     tRNA            complement(597..681)
                     /locus_tag="OO199_003946"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO199_003947"
     tRNA            complement(690..765)
                     /locus_tag="OO199_003947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO199_003948"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO199_003948"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5537795.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO199_003949"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO199_003949"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5537796.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO199_003950"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO199_003950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5537797.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1039 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJTNV010000195         4129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNV010000195 DAJTNV010000000
VERSION     DAJTNV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..440
                     /gene="tnpA"
                     /locus_tag="OO199_003951"
                     /pseudo
     CDS             1..440
                     /gene="tnpA"
                     /locus_tag="OO199_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO199_003952"
                     /gene_synonym="qseD"
     CDS             complement(558..1502)
                     /gene="hypT"
                     /locus_tag="OO199_003952"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5537798.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1847..2566
                     /locus_tag="OO199_003953"
     CDS             1847..2566
                     /locus_tag="OO199_003953"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5537799.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2632..3930
                     /locus_tag="OO199_003954"
     CDS             2632..3930
                     /locus_tag="OO199_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5537800.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1043 a          896 c         1018 g         1172 t
ORIGIN      
        1 agcgcacacc cgatggaact gtaaatatca catagttttt tgcgccaaaa taccgaagac
       61 aggtgttcta cagagagaag cgtagagcaa caggcagtat tttgagaaag ctgtgtgagt
      121 ggaaaagcgt acggattctg gaagctgaat gctgtgcaga tcatatccat atgcttgtgg
      181 agatcccgcc caaaatgagc gtatccggct ttatgggata tctgaaaggg aaaagcagtc
      241 tgatgcttta cgagcagttt ggtgatttga aattcaaata caggaacagg gagttctggt
      301 gcagggggga ctacgtcgat acagtgggta agaacacggc gaagatacag gattacataa
      361 agcaccagct tgaagaggat aaaatgggag agcagttatc gattccctgt ccgggcagcc
      421 cgtttacgga ccgtaagtaa cgaagttgga tgcaaatgtc agatcgtgtg cgcctgttag
      481 ggcgcggctg gtaagagagc cttataggcg catttgaaaa acctccggct atgccggagg
      541 atatttattg cacgtcgtca attgcggcgt gctggctctt ccattgatat ctgctggatt
      601 aaatcgtctg gcatatgttc ctgtaaaata cgccagaggt tctcggcggt tctattcagt
      661 cgggtgttca ttcggtaaat ataagcctga atggggactg tcaatttagc agcatcaagg
      721 catatcagac gtttggtctg tagttcatcg acgatcgacc agatcggtaa ccaggcaatg
      781 ccgtatccgt tgagcgccat attctttaat aactcgctca tcgaagagat aaatatcgtg
      841 cgcccggtaa tacctccaac ctctgccaga tgacgattga ccaacctgcc catataagat
      901 gtggcggtat agttgagcaa aggaacctgg ggctgagtga tatcgaatac agggtgcccc
      961 tgtgcgtcgg cggcacatac cgggtacaac tcagattcaa acagtttcag gcagcaaaaa
     1021 ggcgattgca taagatcttc atcgcgaaac gaaataataa agtcactctt gccttcgcgt
     1081 aacgtattta cggcctggac aacatcaatg gcttcaacat gatagacaaa ttctccgcca
     1141 taggcggtaa gtgaatgcac cagtttgggt aatacgctca gggatagtga gtgcgcggct
     1201 gcaattttaa tgttgggaac gccgagtaaa ctctggccgt ttaactcccc caaattgcat
     1261 tcaagctgct gtaataagct gcgagtctgg gaatgaaaga gcttgccctc ttcggttaat
     1321 tgtaaagggg tggtcgtgcg atcgaataaa acaacgccga tcgctgcctc cagcgcctta
     1381 attcgtcggc tgaatgcggg ttgcgaaaga ttacgctctt ttgccgcctg agaaaaatgg
     1441 cgacaggctt ccagtgtcag gaaatcgtat aaccattttg tctcgatatt cttcattacc
     1501 atggcacctg tattttcaat ccaaagacat aataaaacat cccggcaata aaaataatgt
     1561 tctcaattag cacaacctgg cctttatcat tcgttttgtg cataacccct tcatttccgg
     1621 tattgacgtt gatatcacat ttcgccaaaa aaacgaaatt tttccgtcag ataaccagaa
     1681 tacatcatca ctatggtgaa taagcggaat gttccatagc tgcatcatgc gtgtcttgca
     1741 tcgccttgta caaccttgca tttctcatcg cccatttcga ctgttaaaaa agagagcagc
     1801 agttatttac cgcgtattgt cattaacgtt ttgagaggca ggtattgtga aaggtttaat
     1861 tggtgtactc ggtggaatgg ggccggcagc taccgtcgac ctgtttaata aatttgtaac
     1921 ttttaccgct gcacaatgtg atcaggaaca tattccttta attatttctt cgatcccgga
     1981 tattcctgac cgtaccgatg cattaatgca tcatgggcgt tcaccattac ccgttatgcg
     2041 tgattatatg cataagctgg aagatgccgg ggcagaatgt attgttattc cctgtaatac
     2101 cgcgcattac tggttcaaag aattaaaaga tgcttgtcat attgatatat taagtatcgt
     2161 agaaactacg atcaatgaag ttcgcgcctg tggtaaaacc cgaattggct tgttagccac
     2221 caatgccacg ctatatatgg gtttatacca aaaaggaatt gagtcgctgg gatttacctg
     2281 tgtcagcccc gatgccgacg gtcagaaaaa agtgatggaa agtatttatt ccttgaaagc
     2341 tggcaatata gcgcacgcac aaaagttgat gaatgagcag gctgaaatat tattttcccg
     2401 cggtgcagaa atccttgttc ttggttgtac agaaattccg gttattctgg cgcaagccgt
     2461 taaagagcag ccttcccgct atattgactc aacggcgtca ctcgttcgtg ccggaataaa
     2521 atggtacgaa aatcgtgtcg gtaaacatta tcttttaacc caataatcat ttaaatcgca
     2581 gccagaaagt tattcgcttt taactgaatt atatttataa cggagaacat tatggtttgg
     2641 ctggaaatta tcgtagtact tggtgcaata ttttttggta ttcgccaggg gggaatcggt
     2701 attggtttat gtggcgggct tgggcttgcc attctgactc tgggatttgg tctgcctatg
     2761 gggtcgccgc cagtcgacgt gatcctgatt attatgaccg ttgttgtggc ggcctcagcc
     2821 ttacaggccg caggcggaat ggattatctg gtacgcgtcg ctggtaattt tatgcggcgt
     2881 aacccgaaat atatcaacat aatcgcgccg attatcacct ggtctatgac cattatggcc
     2941 ggtactgggt ttattgtttt ttccacgctg ccggtgattg ctgaagtggc gaaagagtcc
     3001 ggtatccgtc cttcccgcac gcttgccggt tcggttgtcg cttcgcaggt tgcgatttcc
     3061 ggttcgccga tcagcgccgc aatggcggct atgctcacta ttatggaggt aaacggtgtc
     3121 agttttattc aagtgatgtc ggtatgtctt cccacctctt ttgctgccgc gatggtcgcc
     3181 gcttttattg cttcccgaca gggatgtgag ttgcaggatg acgaggtcta ccttgagcgc
     3241 ctgcaaaaag gactggtgca aaaatacgag aataatagca gcatcaagcc aggtgcgacg
     3301 ctctccgtcg gattgttcct gctggcgacg gtcgctattg ttattcttgc tgccttcccc
     3361 caactgcgtc cgggttttga tatcggtaaa ccgatggaaa cacgcgatat cattattatc
     3421 tgtatgctct ctgccgcctg cctgatggtg gtgctgtgta agacggcgac cgatgccatt
     3481 attctcacct ccacattccg tgcggggatg agttcgctgg cggtaattct cggcattgta
     3541 accttaggca ccacttttat tgatgcgcat ttaactgaga ttaaagatat tgctggtgat
     3601 attttgcagg cgtatccgat gttgttggcg ctggttcttt ttggcacttg cgcgctgctc
     3661 tactcgcaag gtgcgaccac gccgttaatt atcccgcttg ccgtcgcgct tgacgtgccc
     3721 acctgggcaa ttctggcgtc ctatgtggcc gtcaccggag tatttgtact gccaacgtat
     3781 ccaacatcac tggctgccat ggaatttgat actaccggca caactcgtgt aggcaaatat
     3841 gtgctgaatc atccatttat gctgcctggg ttggggggaa ttattgctgg agtggcatta
     3901 ggctttgtga ttgcgccaat gattatttag cactatcaaa catcagtatc gatggtttat
     3961 acacaataaa gacagatatg gcggaatata gtctgttgta agcgccaaca aagaataggg
     4021 gaattccgcc atgtctgaac atcttcacaa tatggtatta agtgttgtcg tttaatgatt
     4081 tatttcttgg ttatgttgtg tattagctgc atctatcgct ttttgatac
//
LOCUS       DAJTNV010000196         4110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNV010000196 DAJTNV010000000
VERSION     DAJTNV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(954..1145)
                     /locus_tag="OO199_003955"
     CDS             complement(954..1145)
                     /locus_tag="OO199_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5537801.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="OO199_003956"
     CDS             1209..1532
                     /locus_tag="OO199_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5537802.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="OO199_003957"
     CDS             1522..1833
                     /locus_tag="OO199_003957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537803.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="OO199_003958"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="OO199_003958"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="OO199_003959"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="OO199_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="OO199_003960"
     CDS             2748..2981
                     /locus_tag="OO199_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5537804.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="OO199_003961"
     CDS             complement(3527..3994)
                     /locus_tag="OO199_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537805.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          938 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAJTNV010000197         4024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNV010000197 DAJTNV010000000
VERSION     DAJTNV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..356
                     /locus_tag="OO199_003962"
     CDS             <1..356
                     /locus_tag="OO199_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5537806.1"
                     /translation="FVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRFH
                     DLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNG
                     TNTAQEENVVYLNAR"
     gene            618..980
                     /locus_tag="OO199_003963"
     CDS             618..980
                     /locus_tag="OO199_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5537807.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            977..1897
                     /locus_tag="OO199_003964"
     CDS             977..1897
                     /locus_tag="OO199_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5537808.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1900..2851
                     /locus_tag="OO199_003965"
                     /pseudo
     CDS             1900..2851
                     /locus_tag="OO199_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2878..3118
                     /locus_tag="OO199_003966"
                     /pseudo
     CDS             2878..3118
                     /locus_tag="OO199_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3115..3420
                     /locus_tag="OO199_003967"
     CDS             3115..3420
                     /locus_tag="OO199_003967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537809.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3654..3998)
                     /locus_tag="OO199_003968"
     CDS             complement(3654..3998)
                     /locus_tag="OO199_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5537810.1"
                     /translation="MPDDAVEISESEWKTLINSQSVTKMITCGENGHPVIVDRPSPTP
                     EQLALINNEKKSALIAEATNVIAPLQDAVDLDMATDDETKLLLAWKKYRALLMRVDIK
                     NTEWPKKPESNL"
BASE COUNT         1164 a          789 c          779 g         1292 t
ORIGIN      
        1 tgtttgtcca caccaaacct gcctaccgaa gcgacggaat aaaaacagca gcggtaagga
       61 agatgagaac cgacagcaac aaggcatgga agggagcgtt aaagcgggca ggcattagca
      121 acttccgctt ccatgacctg aggcatacct gggcaagctg gctggttcag tccggtgtct
      181 ctcttcttgc gcttaaagag atgggaggat gggaaactct cgaaatggtt caaagatacg
      241 cccacctttc agccgggcat ctcaccgagc acgcgagcaa aatcgatgcg attataagtc
      301 gcaatggcac aaatacggca caagaggaga acgtagttta cttaaatgcg aggtaactta
      361 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaattata
      421 agaatccgct tgtaattcaa aggattatcc catcaacact gcgctcacac gtcccaccac
      481 atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtctc
      541 atcagacata gcaagtatcg atcaattgag acttggatga tagacttcat gcctttcaga
      601 gctcattgat taaataaatg ttaaagttat ttgtaaagta cacatctata ggtgtgctga
      661 acacacttat acactgggtg gtttttggtg tatgtatcta taccgcacat accagtcagg
      721 ctctggcaaa cttcacaggt ttcgtagtgg ctgtgagctt tagcttcttc gcgaatgcaa
      781 gattcacatt caaagcatcg actacagcga tgcgctacat gttatacgtg ggatttatgg
      841 gaatattgag tgtgattgtt ggttgggcag ctgataaatg ctcacttcct ccaatagtca
      901 ctcttatcac cttctccgcc atcagtctgg tgtgcggttt cgtctattca aagttcattg
      961 tctttaggga tgcgaaatga aaatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1021 gatacctgtt ttctataaaa cggtacgtga attccaagag ttgaagccat atgaagtaga
     1081 aattgtattc ataaatgacg gaagtaaaga tgccacagag tcaattatta acgcgctggc
     1141 tgtttcagac ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1201 cttatttgca gggttagacc atgcaagcgg cgatgctgta attcctattg atgtcgacct
     1261 gcaagaccca attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgagat
     1321 ggtgcttgct aaacgttcag accgctcaac tgatggacga ctgaaacgta agacagctga
     1381 gtggttctat aaattacaca acaaaataag caccccaaag atcgaggaaa atgtcggaga
     1441 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctgttgcctg agcgcaatct
     1501 tttcatgaaa ggcatactga gctgggtggg tggtcagacg gatgtcgttg aatatgtacg
     1561 cgcagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1621 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1681 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacaccc ttgtctttgg
     1741 taacccagta cgcgggtatc cctccctgct tgtatcaata cttttcttgg gtggagtgca
     1801 actgatcggg attggtgttc tcggtgaata tataggtaga atttacttag aaacaaaatc
     1861 aagacctaag tatattttga agaaaggtag aaaataacca tgagcatatg tattaaacaa
     1921 tcagcattaa aaatattatt ggctttatca gcactcctaa taacttggtt gactaccaga
     1981 tactttccag ttgaaccgga tgttgctaac agcccaattg tatggaggca tattttagag
     2041 aatggtatat catcaatcca cgactggaaa ccaaccgtgg ataattggta tttcaccgtt
     2101 tacccaatta actttttatt ttatatgctg ttaggtgatg atggcttagt agcattaaga
     2161 ttatcaactg cagttttctc tattgctata gtaatagcag cgatgttaac gctacgtaag
     2221 gcattcgggt ttacccctgc tttattttct attatacttc tatctctaat accatatttt
     2281 tcatacactt atggttttgt atcacatcct ttttctcata attcaacaaa cgctttcggc
     2341 tttttatgct tgcttatatc tgttttcaac atacagtata aaaatatatt tatcactctc
     2401 ctcttaagtt tgacagcatt attttccagc gtatccgatc cgtggtttac agctgcattt
     2461 tttattccac tattaatatc ttacttttta ttctctgtat gggataaaaa attatttaag
     2521 catactgcat taatactatt tgcatgttta atctctcttt ccaatgtatt gcaaaatcta
     2581 cttaacatac ctccccacca atttgaaatt gtatcactca atgatatgat tttaaatgca
     2641 aaatggtgca ttctgttaat aggaaaaagt cttaatttat tagttgtcga caataacgca
     2701 acatcttacg cctcatttgt catttggttc attgccatta ttacatctgc gtggtttgtt
     2761 ttatcggaca ataaaaaaaa cacataccgt atttatattg tgttattttc attattatct
     2821 atcgcaggta ttgtctcatc attcatactg agctataaat cccctgatta tattagcatg
     2881 cgtttcttta tgaatgttac ctgtttcgca ctgatattgt gctgtatcgg tacatcaaca
     2941 aaagcaaaga tattatttta tctaatcgca tttttatttt cgatcagttc aattaaatct
     3001 tacacaaata atgcttcacc attacacgat caggagaaga ttgttaaatc atacattgat
     3061 tttttaaaaa aaaataatct ccattatggg tacggctctt tttgggattt gtcaatgact
     3121 gtaaattggt tatctggtgg ggacatacag ataactcctg ttttctttaa tgctgattcg
     3181 gggaaaatta actttactgg cgtgagacaa cagacactgg cttcttggca tagcaaggag
     3241 gcgttcaatt ctgctcctga gcgacagttt atagcagttt ccatagctaa cgaacctgaa
     3301 cgatgtaaag aaatgacatc ctgccttgca ggtattcaag aacaattagg taagcctgac
     3361 gaagttttga actttgaagg aagagtaata ttagtattta acaaaaaact taacctatag
     3421 taaattgccc cctaccatat aaaccacact aacgaatgaa taacccgtct ttttcgacgg
     3481 gttatatccc tatcaggaat aaaaatggag tggttgccgt atacaacaaa ccctcattta
     3541 gagctatatt ctagagtata tgagatacta taaagttgaa ggagctatca ttagaaaaac
     3601 catatgctgt tataattaga tgtctcgctt ttcatccaat aacgaatttt ctcttataga
     3661 ttgctctctg gttttttcgg ccactctgta ttttttatat caacacgcat caatagtgcg
     3721 cgatattttt tccatgccag taacagtttc gtttcatcat ctgttgccat atctaaatca
     3781 accgcatcct gaagcggcgc tattacattc gttgcctctg ctatcagtgc agatttcttt
     3841 tcattattta ttaaggctaa ttgttctggt gttggagaag gacggtcaac aatgacaggg
     3901 tgaccattct caccacaggt aatcattttt gttacactct gcgaattaat cagtgttttc
     3961 cactctgatt ccgagatttc cactgcatca tcaggaatat tggtgccgtg aaattcagtc
     4021 gaat
//
LOCUS       DAJTNV010000198         3847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTNV010000198 DAJTNV010000000
VERSION     DAJTNV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.199"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1153
                     /locus_tag="OO199_003969"
     CDS             <1..1153
                     /locus_tag="OO199_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5537811.1"
                     /translation="MPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRAD
                     DLRDERSYRRRIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVS
                     DAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAG
                     FDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTT
                     GFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKE
                     EKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSF
                     SIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            1198..1422
                     /locus_tag="OO199_003970"
     CDS             1198..1422
                     /locus_tag="OO199_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537812.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1400..1900
                     /locus_tag="OO199_003971"
     CDS             1400..1900
                     /locus_tag="OO199_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5537813.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1900..3318
                     /locus_tag="OO199_003972"
     CDS             1900..3318
                     /locus_tag="OO199_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5537814.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3318..>3847
                     /locus_tag="OO199_003973"
     CDS             3318..>3847
                     /locus_tag="OO199_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537815.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGS"
BASE COUNT          970 a          948 c         1001 g          928 t
ORIGIN      
        1 gatgcctgtt gagcaggaag cgccaaccca gactggctgg gatttaactg gcaacgcaac
       61 cgggattctg gaactctccg tgaagtgcaa catgggcgat ccggataacg atttcttcga
      121 gcttcgtgca gatgacctgc gtgatgagcg ttcttaccgt cgccgcatcc aggcatccgc
      181 caaaaaactg gcgaataaca ttgagtcagc gattgccaaa caggcaactg aaatgggctc
      241 gcttgttgtt cacgataccc gcgcaattgg tccatcgact ggcctgcctg gctgggattt
      301 tgtgtctgat gcagagcgac tgatgttctc ccgtgagcta aaccgcgaca tgggcatcag
      361 ttacttcctg aaccctgacg attaccgcaa agcaggccgc aacctggtag atggtgacat
      421 cttcgggcgc gttcctgaag aagcatatcg aaacggtact attcagcgtc agattgctgg
      481 ctttgatgaa attcttcgct caccgaaact tccggcagtt accaagtcaa ccgcgactgg
      541 tgtaactgtt tctggtgcgc agaagtttaa gccgcaggca tacacccttg ataccgatgg
      601 taacaaagag aacgtcgaca accgtgttgc aacggtgacc gtatcctcca ccaccggatt
      661 taagcgcggc gacaaaatca gcttcattgg tgtgaagttc ctgtctcaga tggcgaagaa
      721 tgtgctgact gatgacgcga ctttctcaat cacccgtgtg atcgatggta ctcacatcga
      781 aatcacgccg aagccgattg cgctggatga cgcgtcactg acaaaagaag agaaggctta
      841 cgctaacgta aacacctctc ttgctgatac cactccggta aacgttctga acgtggcaac
      901 aaccaccgct aacgtgttct gggctgatga ctcaatccgc ctgctgtctc agccgatccc
      961 ggtaacccat gaactgtttg ctggcatgaa aacgtcttcc ttcagcattc caggtattgg
     1021 tgttaacggc atcttcgcaa cgcagggtga tatcaacact ctgtctggta agtgccgtat
     1081 tgctgtgtgg tattcagcat gtgctgtacg accagaggca attggtgttg gtctgcctaa
     1141 ccagaccgcg tgataaccag agggagcttc ggctcccgtt tttatctgga gacaagcatg
     1201 acacacatga tctttcgtca tggcgacatg aagaagtgga aaggcgttgg ctacgacttt
     1261 gaaatcgtga aagccgaaga gcttcaggaa tatctggatg ctggctggtt ttcacatcct
     1321 gatgaccttt tgaaggatgt tgcagagcca gaagaaaaac agtgtaaaaa gcctggtcga
     1381 aaacctaagg cggcagcaga tgaacctgac aacgaagggt gatttagttc ttgcggcatt
     1441 acgtaagctc ggtgttgcat caaatgccac gttaaccgat gtcgaaccgc agtctatgga
     1501 agacggcgtc aacgaccttg aaatgatgat ggctgaatgg cttggcggtg atgcgtcacc
     1561 tggtatcaac gttggctaca tttttgctga tgcagatgtt gctccggatc cgggcgatga
     1621 gcacggttta tcaaataacg ctatcaatgc cgtcattttc aaccttgcct gccgaattgc
     1681 tccagattat gcgctggaag cgtctgcaaa acttataacc actgccagat acgggaaaga
     1741 gcgactcgtc aaactgtctg caatggacag ggcaaaagca gctaaatgta agtccggtta
     1801 tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg aagtggaatg gttggaatta
     1861 cttccaccga aaggaacctt gcgataacgg gagcgaataa tgcctattca gcaacttccg
     1921 cttatgaaag gtgtcggcaa agacttccga aacgccgact atatcgacta tctgccagtg
     1981 aatatgctgg ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg
     2041 ggcattgcca aacgttctga cgtgaacggt gtatctcgcg gcgtcgagta caacatggcg
     2101 cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca aaggagaaag tgaagtcggt
     2161 gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc ggacatcaca ggcggtaggc
     2221 gttaatggtc aactggtcga gtatcgctat gatggcacgg tcaaaaccgt ctcaaactgg
     2281 cctacagaca gtggattcac tcagtatgag ttaggttcag ttcgcgacat tacgcgctta
     2341 cgtgggcgtt atgcgtggtc aaaagacgga actgattcat ggtttatcac tgaccttgaa
     2401 gacgaatctc atcctgaccg ttacagcgca caatatcgtg ccgagtctca gcctgacggc
     2461 atcatcggta tcgggacatg gcgagacttc atcgtctgct ttggttcatc gactattgaa
     2521 tatttctccc tgactggcgc aaccaccgtt ggtgccgctt tgtatgttgc acaaccatcg
     2581 ctgatggtgc agaaaggcat tgccggaact tactgcaaaa cgccattcgc tgattcgtat
     2641 gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt atatcatcga ctccggtcag
     2701 gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc gctcctacac tgctgatgaa
     2761 ctggctgatg gtgtgatgga atcgctgcga tttgatgctc atgagttgct gattatccac
     2821 cttccgcgtc acgtcctcgt gtacgacgca tcttcaagcg ccaatggccc acaatggtgt
     2881 gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta tcgacttcgt ttacgaaggc
     2941 aatcagataa cgtgcggcga taagctggaa tctgttaccg ggaaactgca attcgatatc
     3001 agcagccagt acgacaagca acaggaacac ctgctgttta ctccactctt caaagcggat
     3061 aacgccagag tgttcgacct tgaggttgaa tcgtcaactg gcgttgcgca gtacgccgac
     3121 cgcctttttc tctctgcaac cactgactgc atcaattacg ggcgtgagca gatgattgag
     3181 cagaatgaac cgttcgttta cgacaagcgt gttttgtgga agcgagtagg gcgcatcagg
     3241 aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac ctgtcactct gtctggcgct
     3301 cagataagga ttgagtaatg gctgattcga atctcaatgt gccggtaatc atccaagcta
     3361 cgcggctcga tacatcagtc cttccacgca atatcttctc gcagtcgtat ctgctttacg
     3421 ttatcgcaca gggcactgat gttggtaacg tggctaacaa agccaacgag gccggacagg
     3481 gcgcttatga tgcacaggtc aggaacgatg agcaggatgt gattctcgct gaccatgagc
     3541 agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga ggagcgaatc agacaggcag
     3601 aatcaactct tcaggaccat gaaacacgaa tagctcagaa tgaaagcgat atttcgtcgc
     3661 ttgacacaag agttcagtcg ctggagtcgc aggtttcaga ccatgaaacg cgcatcgatg
     3721 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
     3781 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
     3841 gctcgtt
//
LOCUS       DAJTNV010000199         3702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTNV010000199 DAJTNV010000000
VERSION     DAJTNV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.200"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            172..1275
                     /gene="proB"
                     /locus_tag="OO199_003974"
     CDS             172..1275
                     /gene="proB"
                     /locus_tag="OO199_003974"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5537816.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1287..2540
                     /gene="proA"
                     /locus_tag="OO199_003975"
     CDS             1287..2540
                     /gene="proA"
                     /locus_tag="OO199_003975"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5537817.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2655..2730
                     /locus_tag="OO199_003976"
     tRNA            2655..2730
                     /locus_tag="OO199_003976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Thr,seq:cgt)
     gene            complement(2745..>3702)
                     /locus_tag="OO199_003977"
     CDS             complement(2745..>3702)
                     /locus_tag="OO199_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5537818.1"
                     /translation="KAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNR
                     RHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAP
                     IIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDM
                     QRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          850 a          930 c          991 g          931 t
ORIGIN      
        1 aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca actgccgcta cgcttgctga
       61 tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt ggcaaccgac gacagtcctg
      121 ctaaaacgtt cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac
      181 agccagacgc tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgtctg
      241 aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat
      301 cggattgtta ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg
      361 gaactgccag cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg
      421 attcaactgt gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg
      481 acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg
      541 ttgctcgata acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag
      601 attaaggtcg gcgataacga taacctttct gcgctggcgg cgattctggc gggtgccgat
      661 aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg ctgatccgcg cagaaatccg
      721 caggcagaac tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgccggt
      781 gacagcgttt caggcctcgg aactggcggc atgagtacta aattgcaggc cgctgacgtg
      841 gcttgccgtg cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt
      901 gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa
      961 aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg
     1021 gcaactgccg ccattctgga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg
     1081 actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc
     1141 gcccacggtg tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg
     1201 caagaaattg atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac
     1261 atgattaccc gttaaggagc tggccgatgc tggaacaaat gggcattgcc gcgaagcaag
     1321 cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg
     1381 ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg
     1441 acgcgcgagc caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac
     1501 ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc
     1561 aggtaatcga tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc
     1621 tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc
     1681 tgtgcctgaa aaccggtaat gcggtgatcc tgcgtggtgg taaagaaacc tgtcgcacta
     1741 acgcggcaac ggtggcggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg
     1801 ccgtgcaggc gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata
     1861 aatacatcga catgctgatc ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac
     1921 agtcgacaat cccggtgatc acaggtggta taggcgtatg ccatatttac gttgatgaaa
     1981 gtgcagagat cgctgaagca ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca
     2041 catgtaatac ggttgaaacg ttgctggtga ataaaaacat cgccgatagc ttcctgcccg
     2101 cattaagcaa acaaatggcg gaaagcggcg tgacattaca cgcagatgca gctgcgctga
     2161 cacagttgca ggcaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg
     2221 agtttctgtc attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc
     2281 atattcgtga acacggcaca caacactccg atgcgatcct gacccgcgat atgcgcaacg
     2341 cccagcgttt tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt
     2401 ttaccgacgg cggccagttt ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc
     2461 acgcgcgtgg cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg
     2521 attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa
     2581 ggccattgac gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa
     2641 catgtccttt cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg
     2701 tcgtaggttc gactcctatt atcggcacca ttaaaatcaa taagttacac atcattagta
     2761 ccttccttat tttttgactg ggacaaattt gggaccgatg ggttcaggat cgagtctatt
     2821 tgccgtgcgt gttcggtaag gtgattaggt gcaaggtgag catatcgacg aaccatttcg
     2881 atagactccc agcctcccat ttcctgtaac actgacaacg ggactccggc ttgaaccagc
     2941 caacttgccc aggtgtgtct caagtcgtga aatctgaaat catcaatacc agcccgtctc
     3001 agcgccgctt tccaggctgt gtttgcgtca taccgcatct tccttactgt tggcgctttc
     3061 gttccgtctg gtttggtaca gctttccttg tacacaaata cccaacggtg atgattcccg
     3121 atttgttttt tcaatacgcg acatgcagta tcattcagcg caacgccaat tgcgcggttt
     3181 gatttactct cttccgggtt tatccatgcc acccggcgct gcatatctat ttgttgccat
     3241 tcaaggttga tgatgttcga gcgtcttaag cctgttgcca gtgcaaattc aacaacagac
     3301 tttaatggct ccggacattc atcaatcagc ctttgtgctt catggggctc cagccagcgg
     3361 atccgtttat tctttggttg aggcacttta ataattggtg ccttatccag cattttccat
     3421 tcacgctctg cggctcttag tagggccttt ataaatgaaa gatgcgtagc cttcgttgca
     3481 acggacgctg gttttggcgt gtattctgga acaggtttcc ctttttttct gcatgcttct
     3541 gccctgagtt tccagttttc ctcatgacgc cggttcgtca ttttctgcat tgctgaataa
     3601 atttttgatt cagtgatgtc tcttagttgc atccctgcga aatgttgaag ccagaatccg
     3661 atccggcttt tgtcatcgtc cagtgatttc ttatgtgctt tc
//
LOCUS       DAJTNV010000200         3682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNV010000200 DAJTNV010000000
VERSION     DAJTNV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            30..509
                     /locus_tag="OO199_003978"
     CDS             30..509
                     /locus_tag="OO199_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537819.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            497..730
                     /locus_tag="OO199_003979"
     CDS             497..730
                     /locus_tag="OO199_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5537820.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            730..2196
                     /locus_tag="OO199_003980"
     CDS             730..2196
                     /locus_tag="OO199_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5537821.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2087..2830
                     /locus_tag="OO199_003981"
     CDS             2087..2830
                     /locus_tag="OO199_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5537822.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2834..>3682
                     /locus_tag="OO199_003982"
     CDS             2834..>3682
                     /locus_tag="OO199_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5537823.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNK"
BASE COUNT          949 a          906 c         1046 g          781 t
ORIGIN      
        1 tcatcaaact gtcttggata gaagccgcag tggatgcgca caaaacgtta aatttcgaac
       61 ccagtggaag aaagcgtatt ggctttgacg tggctgacag tggtacagat aagtgcgcta
      121 acgtttaccg tcacggatcc gttgttttct gggccgacga atggaaggcc aaagaagatg
      181 aattactgaa gagctgccag cgtacttatc aggcggcgct ggagcgtgaa gcagatattg
      241 tttacgactc tatcggtgtt ggtgcgtctg ccggtgctaa attctctgaa attaacgctg
      301 accggaagag cgagaacgca tacgcgcgac gtgtgaatta ccagaggttt aacgccggtg
      361 ctggtgtgca tgagccagat gacgagtaca acggcatccc caacaaagac tttttcgcaa
      421 atcttaaggc tcaggcatgg tggctgaccg tttcagaaat acgtttaacg ccattaacaa
      481 cggagaacag tatcctgtgg atgagctgat cagcatagat tctcgttgtc cgttgcttga
      541 aaagctgaaa ctggaactga caacacctca tcgtgatttc gaccgtaacg gacgtgtgat
      601 ggtcgaaagt aaaaaagacc tcgcaaaacg cgagataccg tcaccaaacg ttgctgatgc
      661 attcattatg gccttcgcgc caatcgatac atcgctggat atctgggaac agctggggag
      721 acaggcctga tggcacgaaa caaacaagcc ctgcggcgaa ctgtgcaggc cacagctgat
      781 ggttatgaga attttattgc ccgagtaggg atgcagacac ctaaccagca ctcagcatcc
      841 acctaccggg ctaatttcac cagtcgtaac cgcatgctgg tggaatggtc ctatcgttca
      901 tcctggatca tcggcgaagc agtcgatgct atcccggatg atatgacccg caaaggcatt
      961 cgcatcactt cggaaattga tgcaaaagat cgtggcattc tcgaatcaca actggatgag
     1021 ttgcaaatct gggatgcgct gaatgacgtg ctgaaatggt cgcgcctcta cggcggcgcg
     1081 gtgggtttca tcatgattga ggggcaggca ccaatgaccc cgctgcgacc cgaaaccatc
     1141 ggtaagggca agtttaaggg gattctcccg ctcgaccgct ggatggtcga cccggcactg
     1201 acccgccgca ttaaagatat ggggccggac ctgggtaaac ctgagtttta cgatgtggtg
     1261 accacagcaa cgggaattcc tgcctggcgc attcatcaca gtcgcctgat tcgctttgat
     1321 ggcgtcacgt tgccatttca gcagaagatg accgagaacg aatggggaat gtcggttgta
     1381 gagcgtatct gggatcgtct taccgcgttc gacagcgcta ctgtcggcgc ggcgcagctg
     1441 gtctacaagg cgcatttgcg tacctacagc gtggagaagc tacgcgagct tatcgcactt
     1501 ggtggtcctg cgtatgaagc gttgctgaag aatatcgacc tgattcgaca gttccagagt
     1561 aatgaaggca tgacgctcat ggactcgcgg gataagtttg aaacccatca gtacagcttc
     1621 agtggtctgg atgacatcct ttcgcagttt gcagaacaga ttagtggcgc tgttggtatc
     1681 ccactggtgc ggttgttcgg acagtccccg aaaggatttt ctaccggtga tgcagacctt
     1741 gccaactatt acgatcgcat cagttcgttg caggagaggc gtttacgtct tccggtgcgg
     1801 cggatactgg acatcatgca tcgttcggaa cttggcaagc cgctgccgga cgatttcacg
     1861 tttgagttta acccgctctg gcaaatgtct gatgtcgatc gctcaacggt ggcgttaaac
     1921 accaccaacg caatcagtac ggcgctgggt gatggtctga tgacactgaa agccgctatg
     1981 actgatttgc gagaaaattc tgacgtaacc ggcatagggg catccattac caacgaggac
     2041 atcgagaatg ccgaagatga agcgccgccc ggcatcggcg aacctgatga cgaaccgcag
     2101 gagccgtcag gcggaaatcc ggtatcgaac cagcctacgc aggatagcga gggcggtcgg
     2161 agacatcgta aatggtcgct acgatggttc aaatgacagt atcacggaaa ttattgcggc
     2221 gctggagcgt tacagtgaaa tcatcacccc ctgggcgaca aaggtcgcgg aaaactttac
     2281 tgcggaccta acccggcaga acgagaaagt ttggcggcaa cacagcaaga acatcagtcg
     2341 cgagctccgc aatcttgtgg aaagcgctcc tgtgggccag gtgatgcaat ccatcatcgc
     2401 cgaacaggtc aagtacatca aatctctgcc tctcgaggcc gcagacaggg tgtacgacat
     2461 ccagaacaaa gcgatagagg ctgttgtcac cggtggtcgg gcggagcagt ttgctaaaga
     2521 gattgcatct accggcgatg ttgctaagtc tagggccgat ctgattgccc gaacggaact
     2581 gggaagagca acgggcgcgc tggatatggc ccgagcgata gctattggct cggatggtta
     2641 tatttggcgt acagccgatg atggcgatgt ccgagattcc cacgatcaca tgaaaggtaa
     2701 attcgtccgc tgggattcac ctccaacttt ggacggcatg accggccacg cgggcgagct
     2761 gccaaactgc cgctgctata aagagatcgt gtttgttcgc gttccattcg caatgaaaag
     2821 ggcagcataa cccatgaaat acttttttga gaccaggctc ggggaaaccc gataccgcct
     2881 ggctgacggc tcgttgctgt gcaaagacgt gccgatagga cgaacaggta agcagctcta
     2941 tggtgctgat gacctgccaa aactgaaacc cgataagttc gttgaaatag tcgtcacgcg
     3001 ttctcctgag caggtattcc atccggccac gcttgcctca ttcgaaggga tgagcatcac
     3061 gatcctgcat cctgaagatg aaaacgggaa tgtgcggctg gtaaatcccg agaactggaa
     3121 agtgcttgct gtcgggcacc tccagaatgt ccggcgcggg acgggtgagc agtctgattt
     3181 gatgctggct gaccttatcg tcaaagacga aagcgccatt cagcttatcg aagatggcct
     3241 gcgcgaagtg tcgtgcggct atgacgcgga gtacgagcag accgagccat gtaaagccga
     3301 gcaggtcgat attaccggaa accatgtggc tcttgtccct aaaggcagag ccggaaatcg
     3361 ttgtgcaatt ggagacagag acacaatggc aaatcaaaag aaaaactggt ggaaccgcat
     3421 gcgtgcggcc atcaagacag gagatgccga caccatgaac gaactggtgg agtcggctcc
     3481 cgcatcggtt acaggagatg agggggattt gccgcagggc gttaatctca acatcaacct
     3541 gtccccgcag caaccactac cggacaaagc accagagatg ggcggagatc caaccggcga
     3601 cagtgatgat gacctcaaaa cattactgaa agccctgctg gctaagctgg aaagaaatgc
     3661 cacgggcgat aacgataata ag
//
LOCUS       DAJTNV010000201         3550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTNV010000201 DAJTNV010000000
VERSION     DAJTNV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.202"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO199_003983"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO199_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5537824.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO199_003984"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO199_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5537825.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO199_003985"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO199_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5537826.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          821 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggcgg
//
LOCUS       DAJTNV010000202         3534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNV010000202 DAJTNV010000000
VERSION     DAJTNV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.203"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..437
                     /locus_tag="OO199_003986"
     CDS             <1..437
                     /locus_tag="OO199_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5537827.1"
                     /translation="LVVASVLVFSLAGCGDKEESKKFSANLNGTEIAITYVYKGDKVL
                     KQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYAQENVTIDME
                     KVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(478..948)
                     /locus_tag="OO199_003987"
     CDS             complement(478..948)
                     /locus_tag="OO199_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5537828.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(995..1714)
                     /gene="yehT"
                     /locus_tag="OO199_003988"
     CDS             complement(995..1714)
                     /gene="yehT"
                     /locus_tag="OO199_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5537829.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1711..3396)
                     /gene="btsS"
                     /locus_tag="OO199_003989"
     CDS             complement(1711..3396)
                     /gene="btsS"
                     /locus_tag="OO199_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5537830.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          935 a          921 c          807 g          871 t
ORIGIN      
        1 ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac aaagaagaat
       61 cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat gtctacaaag
      121 gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc attggtgcaa
      181 ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac aaaaacatcg
      241 cgggtgttga agaaaaatca acctataccg atacctacgc gcaggaaaac gtgactatcg
      301 atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac gtttctgctg
      361 aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa gccgctggtt
      421 ttaaagaagt gaaataatct gtcggcggcc atgtttcgca tggccgccat acccgtctta
      481 gcttttcttc acatgctgac gcgctgccag tccacgcaga aaataacgta aaaattgatc
      541 gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat
      601 cgaaacgcgg aactgctgtt cggtgaggat agccagaatg tcatcggttt tcagcgaaaa
      661 cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg gctccagtgc
      721 cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga atgacgacaa
      781 aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat
      841 ctgttccgcg gtggcttcga cattatccag cgccagaata cgcaccaggt cattattatt
      901 ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttatcatga ggccttcagg
      961 tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc
     1021 ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct
     1081 ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat
     1141 ggcagcgcag tagtggtgta cgactttcca aggtacgtaa tgtcaattcg gtaaagccct
     1201 ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat
     1261 ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct
     1321 gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca
     1381 gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt
     1441 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
     1501 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
     1561 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
     1621 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta
     1681 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa
     1741 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa
     1801 ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg
     1861 ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc
     1921 gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc
     1981 cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc
     2041 aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac
     2101 atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa
     2161 aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc
     2221 tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt
     2281 gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc
     2341 cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaccggtt
     2401 tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac gcaacggaat
     2461 taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt
     2521 gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat
     2581 cggtttgccg ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg
     2641 atcggtaatc gcgactgcac caatatccag ctcctgatac agtacctgag ccactttcat
     2701 gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag
     2761 cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat
     2821 acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag caatattact
     2881 caccagacgc accgcatctt cataaggtcg ggcgatcgca aggatgatca gcatttgcac
     2941 catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg
     3001 cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggttga
     3061 gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc cgccggtcag
     3121 accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc
     3181 acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca tgatgcagaa
     3241 gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa
     3301 cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa cgcacatctg
     3361 ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca cttcccttta
     3421 aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag aaaaatgaga
     3481 attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct atag
//
LOCUS       DAJTNV010000203         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNV010000203 DAJTNV010000000
VERSION     DAJTNV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            102..3005
                     /locus_tag="OO199_003990"
     rRNA            102..3005
                     /locus_tag="OO199_003990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO199_003991"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO199_003991"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          701 c          992 g          657 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DAJTNV010000204         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNV010000204 DAJTNV010000000
VERSION     DAJTNV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.205"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO199_003992"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO199_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5537831.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO199_003993"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO199_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaac atcatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctctttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgatggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataattc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTNV010000205         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNV010000205 DAJTNV010000000
VERSION     DAJTNV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.206"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..262)
                     /locus_tag="OO199_003994"
     CDS             complement(<1..262)
                     /locus_tag="OO199_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5537832.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNND"
     gene            complement(356..918)
                     /locus_tag="OO199_003995"
                     /pseudo
     CDS             complement(356..918)
                     /locus_tag="OO199_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1122..1328)
                     /locus_tag="OO199_003996"
     CDS             complement(1122..1328)
                     /locus_tag="OO199_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5537833.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1435..1998)
                     /locus_tag="OO199_003997"
     CDS             complement(1435..1998)
                     /locus_tag="OO199_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5537834.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT          972 a          672 c          593 g          832 t
ORIGIN      
        1 catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc acagcttccc
       61 tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt cgcttacact
      121 gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc agagaaggaa
      181 ttgccttatt cataccaggg caaggtatta atttaaactg taataattta attttaggat
      241 gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg
      301 aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca ataggtcaaa
      361 aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc gtcaaataat
      421 ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta atatccagga
      481 tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt tccaacccta
      541 aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc tcatagtttc
      601 ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac atttaaagga
      661 ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc gtttgccttg
      721 ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac ggaaacgaga
      781 tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt caatcacagg
      841 agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa tattcgtatt
      901 gatagttctc agagatatat cacactctag atattttaat taagtatcag ttcgatatcg
      961 gcatattgta cactacctca ggtagatgat gagatcagat acagggatca actgacggtg
     1021 agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc attcgttagc
     1081 caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag ggcccataca
     1141 tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt ggataatacg
     1201 aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg agcttcaagt
     1261 aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct gtcagattta
     1321 taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact gaaattcgtg
     1381 tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca taattcagtt
     1441 atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca gaataatacc
     1501 gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt cgtctaccgc
     1561 agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt ctttagcccg
     1621 gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc ccagttgcct
     1681 ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa aatgaatatg
     1741 aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca tcgtctgggt
     1801 ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat gcccgaaact
     1861 gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag aaaaacgttg
     1921 aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca ttttgtaatt
     1981 gggattggca ttatacattt tatagtcctt actttataaa actcacagga agagagaatt
     2041 acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga agtaaaccag
     2101 cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat gaggtaatac
     2161 ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa tggaaaaaca
     2221 cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa gtaatctgac
     2281 attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat tgtcgaacca
     2341 ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga ataatggtat
     2401 acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc atatttacct
     2461 atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat acgtaaccag
     2521 tgttaagtac gtacaggaca acattacaca cataattcac catacagctt cccccatatg
     2581 cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac ttagcataaa
     2641 gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa acaatacggc
     2701 acagacacac aataccataa ctccaactaa taatactaat taaccttaat tatttatggt
     2761 atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga tgatcactgt
     2821 ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg agtgaccagt
     2881 aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata agccactaac
     2941 ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat catgatttgg
     3001 cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca agagcttaca
     3061 ccgaattac
//
LOCUS       DAJTNV010000206         3066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTNV010000206 DAJTNV010000000
VERSION     DAJTNV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.207"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="OO199_003998"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="OO199_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5537835.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="OO199_003999"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="OO199_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5537836.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3066)
                     /locus_tag="OO199_004000"
     CDS             complement(2711..>3066)
                     /locus_tag="OO199_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5537837.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          721 a          677 c          753 g          915 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa atgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataat
//
LOCUS       DAJTNV010000207         2926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNV010000207 DAJTNV010000000
VERSION     DAJTNV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OO199_004001"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OO199_004001"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5537838.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVCIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OO199_004002"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OO199_004002"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5537839.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="OO199_004003"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="OO199_004003"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5537840.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="OO199_004004"
     CDS             complement(1896..2810)
                     /locus_tag="OO199_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5537841.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          770 a          678 c          782 g          696 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttgtatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct agaaccacgg cagcctgacg gctgcaaatt gaaaac
//
LOCUS       DAJTNV010000208         2876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNV010000208 DAJTNV010000000
VERSION     DAJTNV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.209"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..473
                     /locus_tag="OO199_004005"
                     /pseudo
     CDS             1..473
                     /locus_tag="OO199_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            466..2543
                     /gene="flhA"
                     /locus_tag="OO199_004006"
                     /pseudo
     CDS             466..2543
                     /gene="flhA"
                     /locus_tag="OO199_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2543..>2876
                     /gene="flhe"
                     /locus_tag="OO199_004007"
     CDS             2543..>2876
                     /gene="flhe"
                     /locus_tag="OO199_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5537842.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
BASE COUNT          604 a          712 c          878 g          682 t
ORIGIN      
        1 attcgtgatg agttcaaaca aggcgaaggc gacccccatg ttaaaggacg gatctgtcag
       61 atgcagcgag ctgccgcacg gcgtcggatg atggccgatg tgccgaaagc ggatgtcatt
      121 gtcaataacc cgacccacta ttcggtagcg ttgcagtatg acgaaaacaa aatgagcgca
      181 ccgaaagtgg tcgctaaagg tgcagggctg gtcgcgctgc gcattcgtga aattggcgct
      241 gaaaataacg tcccgacgct tgaagcgccg ccgctggcgc gagcgctgta tcgacatgcg
      301 gagattggtc aacaaatccc gggtcaactg tacgccgcgg tggcggaagt gctggcctgg
      361 gtctggcaac tgaaacgctg gcgtctggct ggtggacagc gccctgtaca cctactcatc
      421 ttccggtgcc ggcagccctg gattttatta acgagaaacc gacccatgag taatctggcc
      481 gcgatgctgc gcctgcccgc aaacctgaaa tcgacataat ggcagatcct tgccggaccg
      541 attttgatcc tgttgatctt gtcgatgatg gtgctgccac tgcccgcatt catactcgac
      601 ctgttgttta ccttcaatat tgccttgtcg atcatggtgt tgctggtggc gatgtttacc
      661 cagcgcacgc ttgagtttgc tgcgtttccg accattctgt tgtttactac gttgttgcgt
      721 ctggcactta acgtggcttc aacccgtatc attttgatgg aagggcatac cggcgcggcg
      781 gcggctggga aggtggtcga agcgttcggt cacttcctcg ttggtggcaa tttcgctatc
      841 ggtatcgtgg tgtttgtcat tctcgtgatc atcaacttta tggtcattac caaaggtgcc
      901 gggcgtatag cagaagtggg tgcgcgcttt gttctcgatg gtatgccggg taagcagatg
      961 gcgattgacg ccgaccttaa cgctggattg attggtgaag atgaggcgaa aaaacgccgc
     1021 tccgaagtga ctcaggaagc cgatttttac ggctcaatgg acggggcaag taagtttgtt
     1081 cgcggcgatg ccattgccgg gatcctcatc atggtcatta acgttgtcgg cgggttgctg
     1141 gtcggcgtgc tgcaactggc atgagcatgg gacacgcggc ggaaagttat acgctattga
     1201 ccattggcga cggtctggtg gcacaaattc cggcgctggt gatttctacc gccgcggggg
     1261 gcatcgttac gcgtgtcagc accgatcagg atgttggcga gcagatggtg aatcagcttt
     1321 tcagtaaccc aagcgttatg ttgttaagcg ccgccgtgct cggtttactc ggcctggtgc
     1381 ctggaatgcc gaacctggta tttttgctgt tcactgccgg attgctgggg ctggcctggt
     1441 ggatacgcgg acgcgaacaa aaagcgcctg ccgaacccaa accggtaaaa atggcagaga
     1501 ataataccgt tgtcgaagcg acgtggaacg atgtacaact ggaagattct ctgggaatgg
     1561 aagtgggtta tcgactgatc ccgatggtcg atttccagca ggatggtgag ttgttgggcc
     1621 gtatacgcag tatccgcaag aaatttgccc aggagatggg atttctgccg ccagtggtgc
     1681 acattcgcga caatatggat ctgcaacctg cccgctatcg cattttgatg aaaggcgtgg
     1741 agattggcag tggtgatgct tatccggggc gctggctggc gattaaccct ggaaccgctg
     1801 ccgggacgtt acctggtgag gcgaccgtcg atccggcatt tggcctgaat gctatctgga
     1861 ttgaaagtgc gctaaaagaa caggcgcaga ttcaggggta cacagtggtt gaggccagca
     1921 cggtggtagc aacgcatctt aaccacctca ttagccagca tgccgcagag ctgtttggtc
     1981 gtcaggaggc gcaacagctg ttggatcgcg tcgcccagga gatgccaaag ctgacggaag
     2041 atctcgttcc tggcgtcgtc acgctcacca cactgcataa agtgctgcaa aatctcctcg
     2101 atgaaaaagt accgattcgc gatatgcgca ccattctcga aacgctggcg gaacatgcgc
     2161 ccatccaaag cgatccacat gaattaaccg ccgtcgtgcg cgtggcgttg ggacgggcga
     2221 ttacccagca gtggtttcct ggcaaagatg aagtccatgt tattggcctc gatacaccgc
     2281 tggaacgttt gttactacag gcgctgcagg gcgggggagg actggagcca gggctggcgg
     2341 atcgtttact ggcgcaaact caggaagctc tatcccgtca ggagatgctg ggtgcgccgc
     2401 cagtattgtt ggtgaaccac gcgctgcgac cattattgtc tcgcttcctg cgccgcagct
     2461 tgccgcagtt agtggtcctg tcgaatctgg aactgtctga taaccgacat atccgcatga
     2521 cggcgacaat tggcggtaaa tgatgagaac cttattagcg atattattgt ttccgctgct
     2581 ggtgcaagcc gccggggagg ggatgtggca ggcaagtagt gtcggaatta cgctaaatca
     2641 tcgcggtgag tcgatgtcgt ctgcacctct ttctgcgcga caacctgctt caggattgat
     2701 gacgctggtg gcgtggcgtt atcagcttat cggcccgaca cctgcaggac tgcgggttcg
     2761 cttgtgttcg caatctcgtt gtgtcgaatt agaggggcag agcggtacca ccgtggcctt
     2821 ttccggtata gcggcagcag aaccgttgcg atttatctgg gaagtgccag gcggtg
//
LOCUS       DAJTNV010000209         2844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTNV010000209 DAJTNV010000000
VERSION     DAJTNV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..670
                     /locus_tag="OO199_004008"
     CDS             <1..670
                     /locus_tag="OO199_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5537843.1"
                     /translation="STVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRI
                     NARLEDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRW
                     ANTSDWGWKEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDA
                     FFCTFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREEL
                     ETML"
     gene            667..1572
                     /locus_tag="OO199_004009"
     CDS             667..1572
                     /locus_tag="OO199_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5537844.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            1569..2639
                     /locus_tag="OO199_004010"
     CDS             1569..2639
                     /locus_tag="OO199_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5537845.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT          695 a          662 c          831 g          656 t
ORIGIN      
        1 atcgaccgtg accagcctgc accgtgaggc tgaagcacag ttccgtcctg aactggaaaa
       61 aatcgtcagg ggtattgaaa caggttttcg tggtacggcc ctgtacgcca cagaaaatat
      121 tgccggacgc atcaatgccc gtctggagga tgaaggtttc actgtaaaaa tcagttttcc
      181 ggcagtcagc cagttacaga cccggctcgc agtaaaaaca aatctgagcg cgcttatgga
      241 ggaaagaacg gagacagtaa cccgtcgctg tcgacaggat ggtgtctggg gtacgctttg
      301 tcgatgggca aacaccagtg actggggctg gaaagagtac agtgtggatg tgagccgcag
      361 cgtgatcaat atcaacaagg tcagagagga agtgatgtca ctgacccggg catatttcgg
      421 ggagctgcag gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc
      481 ctttttctgt acattcaggg agaaagttga acagctgcgt aacacgctga ttcagagctc
      541 tgaagatcat aaacgcgatc agcaggcgca ggaacggctg accgggcgtc ttcaggcgtt
      601 aaatgaaagg gttcctgagc tcattactga cagtaaggcg ttaagggaag aactggagac
      661 aatgctgtga cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc
      721 acctgtcttc ctgaaaaatt tgtggttgat ttcgcaaacg gtattgatgt tgcacaggag
      781 catatccgta cggccggaga gcgcacgttc ttccggcggt taaaagaggg gctgagcggc
      841 gaaggtactg cacggcagaa tgccatcaat gcttcgcttg cacagggcgt tgaggcgtcc
      901 ctgcgctggc tgacggagct gacaacgtca cttgccacca caaactacgc gattacccgg
      961 gtaaacgaca gggtcagttc actggtcagt gatactgcca ggctggcgca ttattccgca
     1021 gatacgcggg agcagttact caccctggcc gatcaggttc accagaaact gaatcatctt
     1081 gaagaaaaac tccaccgtgt tgacctggtt cagcgggcgc agttacatct tgagcagata
     1141 ttctcctggt ggagcgccgg gcgatatgcg tcattttccc ctgccggacg ttgttatgtg
     1201 gcacttgaag agcttcgctg gggcgcgttt ggtgatgtga tacgtcaggg cgaaacaggc
     1261 caggttaacc agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag
     1321 agtggcggta gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg
     1381 gagcaggcag ataacgagtg gcatgacgcg attaactggc tgggggactg gtgcagtgaa
     1441 gagcagcatc cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg
     1501 ccccgtctct gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag
     1561 ggggctgcat gagtatagaa ataaaaacag ggctggtgct gtccggcggt ggggcggtgg
     1621 gcgcttatca ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg
     1681 tttcaggggc cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt
     1741 cagaagctgc cgtacgcctg gaggcgctct gggatcatct tgggaataat caggtgctgt
     1801 cggtaaaccg atcggtttac ttttcattgc tgaaaaaatt gttccaggca atgaacctct
     1861 gccagatccc cggacgtgca ggagcactgc ttacgacact tcttcgccat atatcgataa
     1921 tcaacggctt cgacaatccg atgactcagc cgttgttgtc agatgggccc ctgacagcgt
     1981 tgatggatca ttatcttgat acagatgctc tggcaggcgg gctaccgctg tatgtgtcgc
     2041 tgtaccccac agaagggggc atgcaggata ttattgactg cattcgtgct gaactgggtt
     2101 ccggaaccac gaaaaacgcc gtttttcagc atatccagag tctgccccgc ggacagcaga
     2161 aagaggctct gcttgcgtca gccgcactgc ccctgttgtt ccgtccccgt gaggttcagg
     2221 ggacaatgtt cggtgatggt ggtatgggag gatggcgaaa tatgcaggga aatacccctg
     2281 taacgcctct ggtcgatgcc ggatgcaata tggtgattgt gtcgcatctg agtgacggtt
     2341 ctttatggga tcgccaggct tttccggaca ccacaatcct tgagatccgt ccccggaaaa
     2401 ggctgaaata tgcaggtgat ggtggcaaca gtggcggtct gctcagtttc acgtcggcac
     2461 ataccgacgc ctggcgtcag cagggctacg aggacacgat gctgacgatg gagcatatcc
     2521 ggaaaccgct ggcagcacgt caggcactgt cccggtcaga aacggtgttg cagaaaagtc
     2581 tggagataac ggaagaggca gatttggcac tgagaagcgc gatggcccgg attaaataaa
     2641 gacgccccaa agaaacgcca ccgcctgtgg tggtgttttt cctgtctggt gttgttgaaa
     2701 atattttcag atatatatta cctgggtgag atttaagatt aacgcattta ttaatgtcat
     2761 aaggagagtt atcatgaaat tcatcgtaaa agcacttagg tgatgctgcc aacttactga
     2821 tttagtgtat gatggtgttt ttga
//
LOCUS       DAJTNV010000210         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNV010000210 DAJTNV010000000
VERSION     DAJTNV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            6..81
                     /locus_tag="OO199_004011"
     tRNA            6..81
                     /locus_tag="OO199_004011"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO199_004012"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO199_004012"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO199_004013"
     CDS             complement(299..1057)
                     /locus_tag="OO199_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5537846.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO199_004014"
     CDS             complement(1065..2168)
                     /locus_tag="OO199_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5537847.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="OO199_004015"
     CDS             complement(2178..>2807)
                     /locus_tag="OO199_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5537848.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAJTNV010000211         2759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNV010000211 DAJTNV010000000
VERSION     DAJTNV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.212"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            78..143
                     /locus_tag="OO199_004016"
                     /pseudo
     CDS             78..143
                     /locus_tag="OO199_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            371..619
                     /locus_tag="OO199_004017"
     CDS             371..619
                     /locus_tag="OO199_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5537849.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(735..1004)
                     /locus_tag="OO199_004018"
     CDS             complement(735..1004)
                     /locus_tag="OO199_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5537850.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1061..1729
                     /locus_tag="OO199_004019"
     CDS             1061..1729
                     /locus_tag="OO199_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5537851.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2045..>2759
                     /locus_tag="OO199_004020"
     CDS             2045..>2759
                     /locus_tag="OO199_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5537852.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGP"
BASE COUNT          843 a          604 c          541 g          771 t
ORIGIN      
        1 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac tgggacctcg
       61 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
      121 ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca
      181 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      241 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      301 ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
      361 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
      421 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
      481 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga
      541 taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg agtctgctga
      601 cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat cattcaatac
      661 tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg
      721 tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc aggtgcagtt
      781 gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag caacgagttt
      841 tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat atgctcactg
      901 aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
      961 gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt
     1021 catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat taatgaaaaa
     1081 aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg
     1141 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc
     1201 acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta
     1261 ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct
     1321 gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt
     1381 ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct
     1441 tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca
     1501 ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca
     1561 caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac
     1621 aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac
     1681 caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc
     1741 agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt gcgactttac
     1801 ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata tttaactaac
     1861 cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag tatcgttttt
     1921 ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca
     1981 ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct tctgcgaggt
     2041 tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt tttacagaac
     2101 tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat ggactaaaaa
     2161 cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat gtctggagaa
     2221 cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac gattgcctga
     2281 caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg agctaccaag
     2341 ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg aattaccagt
     2401 gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg aattacctgc
     2461 gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga ggttaccaat
     2521 gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag attttccaga
     2581 aagagatgat catattataa gaatattctg gcttaatcag aaccggatca cggcaattcc
     2641 ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa atcaactatc
     2701 tcccagaata atgcaaactt tgttacaaca aaccgcccaa ccggactacc acggcccac
//
LOCUS       DAJTNV010000212         2691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTNV010000212 DAJTNV010000000
VERSION     DAJTNV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            230..769
                     /locus_tag="OO199_004021"
     CDS             230..769
                     /locus_tag="OO199_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5537853.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1319)
                     /locus_tag="OO199_004022"
                     /pseudo
     CDS             complement(995..1319)
                     /locus_tag="OO199_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
     gene            1605..1799
                     /locus_tag="OO199_004023"
     CDS             1605..1799
                     /locus_tag="OO199_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5537854.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          666 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccca gtctcataac ttctttcagc
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaacca
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga cacggggtgt cggggcgaag ccctgaccag ggtaattgta
     1381 atagcgtgca tgtatgcgcg gtataacaat tacacatcct gtcctgtcag caagttcgaa
     1441 ttaccgacaa aggcacggca gtttttttgg cagtgacagt attgtcaggc agaaataccc
     1501 gtcaatgcgt cagacggcag aataccggcg gtaaaacagt cgaaaagtgg tacgcgatat
     1561 aatcacacaa cgcagatgac agacagaacg gagaaaacag aaagatgaac aagcagcagc
     1621 aaaccgtact caacatggcg ggattcataa aaagccagag cctgacgctg ctcgaaaagc
     1681 tggacgcact cgatgccgac gaacaggccg ccatgtgtga gaagctgcac gaaatcgcgg
     1741 aagaactcca gaacagcata cagacacgct ttgaagcgga aagtggcacc ggggtataac
     1801 agtgtatcct gctccagaca ggatgcagaa cggtataccg gtgcgtaagg agaaaatgtc
     1861 acataaggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     1921 ggcgagcggt gtcagctcac tcaaaggcgg taatgcggtt atccacagaa tcaggggata
     1981 acgccggaaa gaacatgtga gcaaaaagcc gaaggcaggg gcaaaggccg cgcaggtggc
     2041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2161 gcgctctcct gttccgaccc tgccgtttac cggatacctg tccgcctttc tcccttcggg
     2221 aagcgtggcg ctttctcata gctcacgctg ttggtatctc ggcccggtgt aggtcgttcg
     2281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2341 taactatcgt cttgagtcca acccggtaag acacgaatta tcgccactgg cagcagccac
     2401 tgataaccga attagaggag taagacttga agtcatgcgc ctgttaaggc taaactgaaa
     2461 gaacaagttt tggtgactgc gctcctccaa gccagttacc tcggctcaaa gagttgatag
     2521 ctctgagaac cttcgaaaaa ccgccttgca gggcggtttt ttcgtttaca ggcagcagat
     2581 tacgcgcaga aaaaaaggat ctcaagaaga acctttaatt ttttttcaca cttggtatcc
     2641 tactttaacc cataaaataa acacttacac cgacacaccc ttaaacaaaa g
//
LOCUS       DAJTNV010000213         2681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTNV010000213 DAJTNV010000000
VERSION     DAJTNV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.215"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1829
                     /gene="kup"
                     /locus_tag="OO199_004024"
     CDS             <1..1829
                     /gene="kup"
                     /locus_tag="OO199_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5537855.1"
                     /translation="AAIGVVYGDIGTSPLYTLRECLSGQFGFGVERDAVFGFLSLIFW
                     LLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGE
                     VVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIML
                     TWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGGEVLY
                     ADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLL
                     IIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYV
                     AVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLC
                     VDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIAS
                     LEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVR
                     RVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGK
                     RPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1996..2415
                     /gene="rbsD"
                     /locus_tag="OO199_004025"
     CDS             1996..2415
                     /gene="rbsD"
                     /locus_tag="OO199_004025"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5537856.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2423..>2681
                     /locus_tag="OO199_004026"
     CDS             2423..>2681
                     /locus_tag="OO199_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5537857.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQ"
BASE COUNT          588 a          645 c          716 g          732 t
ORIGIN      
        1 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
       61 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      121 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      181 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      241 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      301 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      361 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      421 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      481 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      541 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      601 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      661 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      721 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      781 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
      841 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
      901 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
      961 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1021 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1081 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1141 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1201 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1261 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1321 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1381 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1441 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1501 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1561 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1621 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1681 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1741 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1801 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     1861 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     1921 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     1981 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2041 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2101 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2161 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2221 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2281 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2341 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2401 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2461 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2521 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2581 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2641 gaaatcttcc caggaagccg ggattgggat tatccatcag g
//
LOCUS       DAJTNV010000214         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNV010000214 DAJTNV010000000
VERSION     DAJTNV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            63..1541
                     /locus_tag="OO199_004027"
                     /pseudo
     CDS             63..1541
                     /locus_tag="OO199_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1538..>2420
                     /locus_tag="OO199_004028"
     CDS             1538..>2420
                     /locus_tag="OO199_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5537858.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGI"
BASE COUNT          623 a          575 c          602 g          620 t
ORIGIN      
        1 ctcgtgagga tttatgttct gctggcagtt tcgtgatcaa cacctcgcgc caggatggct
       61 ttatgcacta tcgcaatgtg gtttgtttgt tgtcttgttc attgttttta tcgtcggcat
      121 ggggatgtcg gctttccgca cctgaacata atatttacca gaaacaggga aaaggcgtgg
      181 tgtacctgcg cccttataaa gaaacaaatc tttctctacc tgacgtgaat tacaagagtt
      241 tgcgtcggtt acccaattta ttaatagacc cgacaacact agacgaatgg gataaagaac
      301 cgcctctgac tgatttgaca accgactacc tctatgaggg cgcacaggcc tggtatcctc
      361 attattcatg gcatagcgat ggtcgcaata tcctctacgc gggtgaagtt gtgcaaaacc
      421 ctcccggcac acctcccgtc gatgtcgcct cgttcaaggc atggggtgat tttgccgcag
      481 ataaacacag tctctatttt gaaggcaagc gcaccgatga caatggtggt ggaaatagct
      541 tggatatcaa aacgctgcat caggtggaat tccggcctcc ctgggatcct gacttactgg
      601 ggctgatatt acgtgacgct aatttcttgt atatcaatgg gcatcgcctc gctgaccccg
      661 agagtttccg tgtgctggcg cagaagtcct gggatcagcg aggaaagttt tccaccacgt
      721 ttaacccctg cattgccgta ccatttggcc cctgggatac cctggctcgt acacggacca
      781 aaatcctgct caacggcgaa cagcttgatg ccgacccgga caccttttcc gtcgtgcgct
      841 ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg gctgcagcgt aaagtcctcg
      901 acaaggaaaa tctggcgtgg gatgaagatt taacaaagca ctgtctggat ttttctctgc
      961 tggaaaagaa agtgttctgg cgtaaaggac ctgcttgtaa acaggaagaa ttacccggac
     1021 tcgatccgga gcagtttcaa cccatcagtg atgctgtcgc ccagcatcag gactcgcttt
     1081 ataccatcat cgaaacagag tctggtgacc gtaagctgga gatcgtgaaa cttgatgatc
     1141 ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg ccacggctat ttacttacgc
     1201 gtgccgaagg gtagccatac cattccggtt tacacgtgtt tgaatctgac ggaccgctga
     1261 tcttactgga taaccgttct ccggatgaac gcgaagccca tcttaatgac catccctttt
     1321 tgcgcagatg gtatgcccgc gataaccgct acatttacag ctttgatggc gcgcagctct
     1381 ggcgataccg caccgctgat ccgaaacaag ttcgcttaat ctggaaggaa caacattcgg
     1441 gatatgtcta tggcgtaaat tacaaaacgg gatatctgga cggaaaaatt actgatgacg
     1501 gcgaatttat tcctgccccg cgcaatgagg cgacaaaatg aagggtcaaa taatcacagt
     1561 tgcactcatc ctgttgggac acacatttct gacgcctgct gtacaggcca tagggttcga
     1621 ctattacaat gatcacggtg ttatgtctta cggtaaaggt tattgggggg ataagaaaat
     1681 cattgcccag tttccggaga tgagcagggc cggtttgcgc cttgtgaaaa atatctctgg
     1741 gaaaacgggt tatatacctt ccgatgaaat aagcatagaa aatgaggaat accactgggt
     1801 gacagacggc cgcgttattc tctggcgtgg caaaatcgtt agcaatccac cgggaacact
     1861 cactgtcgat attgccagct ttcaggctat gggccgcttc gcggtcgata aatatagcct
     1921 ctattttgac ggacagcgca ccgaaagtaa tagcggcgcg tcacgtgttg agctggcgac
     1981 actaaaagct atcgaaggta actctaccac gctggtggat agtaaaaatc tctacttgtc
     2041 cggtcgacgt caggggagca gcagtgatgt tactgtatta gaaaaaagat ggtggggtat
     2101 taatccacgt cttatgagtg taaacagaaa ttcatattcc aatgatctgc ttatccgcag
     2161 tgggcagaat atttatttaa atggcgttca ccttacggcg aatgcagact catttgagat
     2221 aattcgctgg atacctcatt cactgctggt ttttcgcgac aataagggtc tgcatcgtta
     2281 tccctttggt caattatcag gcaaagcgat acccgtagat gatgacgtct cttttgaagt
     2341 aggggaaagt cgcgttcgct ggcgtaaaca gctcacgccc gaccgtcagt ggagcaagtg
     2401 gatagaccta ccaggtattg
//
LOCUS       DAJTNV010000215         2417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNV010000215 DAJTNV010000000
VERSION     DAJTNV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            122..745
                     /locus_tag="OO199_004029"
     CDS             122..745
                     /locus_tag="OO199_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537859.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(799..1971)
                     /locus_tag="OO199_004030"
     CDS             complement(799..1971)
                     /locus_tag="OO199_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5537860.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          735 a          437 c          497 g          748 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaact tgtgaaggaa atggtactca
       61 gggggattcc agtacaggtg gtgcgcaatt agctataaat ggccttaacg gttcgtgcgc
      121 tttggtatta aacaacccat actttgaggc taatgccggg ggggcagacc ttgctataga
      181 taatactggg acgcgccctg taactgtggt tataaacggt ggaaattttc atcgcgtgag
      241 cagtgcaaga tacacgcaca cgaatattca agtaacaagc tcaggtgggg gaaaagttac
      301 agtcatactc aacggcacaa cgttccagag cgcaggggat tatcagccaa gtgcagaacg
      361 accatactgg ataacaggag ctaattgcga ggtagttgac attgggtgtg tttttacaga
      421 aacaaccagt aaggtaacat cggcttctgc attgtcagtc acacgctcgg gaaaaattaa
      481 cgccaatggt agtatcgacg ttgctactgg tgtttcatcg gttaacgtgg tttctacagg
      541 tgtgtatgat gttagctttt ctcatccgct cgcagcagtt gctagcggtt atattgtgca
      601 gataacacct atatctgcgc ctgattcagt tagttgtgat gttacttata ttggcgtaga
      661 tacatttagg gtgaccctgc gcaatacgct tagcggggca ggaatctcca gttcgtttgc
      721 atttagcatt accagactaa tataaataca ttcgccgccc catttggaca tatatgtagg
      781 gcggcttatg tttattgttt attgattgtt gtttgttaat gtgggtatta attcttttac
      841 taaagttgtg gtttgttttg ttaatttgat gaatggcgtc tctattaatt taaaagttag
      901 gcatgatgtg aacgtaataa gaaatgcagt tgtgcttacc agtatgataa atgtattttc
      961 agtgtaatta ttaggaataa tccacgtcat taggcaataa aggaatatcc catgcagaag
     1021 gtaaacgctg taggtagttt ctcctagctt tctggttata tttaatctta atattccata
     1081 caaatcacaa cctgatgcga taatgataaa tgttattcca caaagaatca gtggtagcgg
     1141 tgcgtaagtt gttttgaaat acgtcatttc aaaaaccatt atcgcagtta ttataattgg
     1201 tgttactttt gctttggcaa ttccattgac tatctttgat ttatttagca aaaatgccag
     1261 gagcccaaaa agaaatgatg ctatgtgtat tttactgaaa aataaaatga aaacaaatag
     1321 cgatattgcg ctaatcataa ccatgtaaat actgactttt ctttttatga gcgcggaaat
     1381 taccggaaga gaaaaataaa agaaccattc atatacaagt gtccatgtta cagcggcatt
     1441 gatagtaaat gaatctttta cgtcattaat gttcggcata cctagcgcag tgaatggcaa
     1501 ccacttcatt attgacacaa aaagctcttc tgtggatacc tgcattctcc atccagactt
     1561 aaaacctaca atgataaaaa tgagacatag gctaactatg aacattgggg ttaatcgtag
     1621 taatcttgat acataaagcc ttgtccagtc ctgatctcca gagataatct ttgaaaagaa
     1681 cagataacca gtaatcataa aaaagaatga cacaccaact tggcctaaat ttgctaacag
     1741 atttgatgaa ggtgcttccc atactccaga tgacaagtat ccattccaaa ttgctgcgtg
     1801 atgaataagc acgaaaattg ctaagaaccc ccgcattcca tctatttgat tattcctgcc
     1861 gtgctcaaca agatttattg gggaaatttt atcaaataac cccacagcta ataacgacat
     1921 caatatcacg gatgttatta ataataaact gttaatttca atcatatgca tgctctatta
     1981 cgcaattaaa atttctccaa gtatactgac ttttagttaa tgatcatata gttatgtgta
     2041 actgatgaaa caaaacggag acacaccaag ctttgcactg gattgcaagg ctttgtgctc
     2101 ttctgtgaat gtgtggctat atgtttgaag atcgttgtgc cgtatttgtg acatatatat
     2161 ggcaacatca tgcatcaact ttctgtttgt gtcatcaact atagcttagt gaatgcggtt
     2221 aatgcttgct aaaacagata gttatgattg gtgctacaga ttcgtaatgc gaaggtcgta
     2281 ggttcgactg ctattatcgg caccatttaa atcaataagt tacacatcat tagtaccttc
     2341 cttatttctt gactgggaca aatttgggac cgatgggttc agaatcaagt ctatttgccg
     2401 tgcgtgttcg gtaaggt
//
LOCUS       DAJTNV010000216         2347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTNV010000216 DAJTNV010000000
VERSION     DAJTNV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.219"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..797)
                     /locus_tag="OO199_004031"
     CDS             complement(<1..797)
                     /locus_tag="OO199_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5537861.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1398..1646)
                     /locus_tag="OO199_004032"
     CDS             complement(1398..1646)
                     /locus_tag="OO199_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5537862.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2001..>2347)
                     /locus_tag="OO199_004033"
     CDS             complement(2001..>2347)
                     /locus_tag="OO199_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5537863.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          606 a          431 c          565 g          745 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggtaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag gtaattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggcg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggggcaatt tttccttaag gtatgaaaaa aggagctgaa gctatataat agctattaat
     1081 gccactggta tcagtgaatc atgtatataa aaaactgtgt taaggaggct gtccattagc
     1141 ggttttttat tcttctggaa ccgcggcatc tttgcgtatc tttttgtctt ctcaccgtct
     1201 ggtcggtgtt ttgctgagac tgctaatttc ctgtctttgc tagtgctgta ctggtactgc
     1261 ccaatcatga ttggcggggg cgggcggagt tgagaccgca gacacgtcgt atgcaagaac
     1321 gtgctgcggc tggctggtga acttccgata gtgcgagtat tgaatgattt ccagccgtta
     1381 ctgattttac gtgctaatta gtgaacaaac cactcgtcag cagactccca ggtatctttc
     1441 agagtttcct gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg
     1501 ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt
     1561 cgggttaatt cttcctttaa cgcatccaca gcaccggttg gcattttagt cattttctct
     1621 ttggctatgc agatttcaaa acgcataaaa atccctctgc actgtgtttt tatacagtat
     1681 tatttttaac tgtatggata aacagtgtca agagtgaggg aaagtgattt tttggggcat
     1741 gcatggggca aaaatatttg ttgtggggca ttttggggca tgattgggac actttgttac
     1801 atatgaactt tgccgatttt catatgacat ggcaatccaa cgctttgatt attctaaaga
     1861 aaacacatgc tcttgggcgt tctttagtga tttttaaaaa tcccgtatca cgcagttaaa
     1921 gtggcgggca tactcttcaa ggctggtgat ccccaaccgc acccatttag gatgtgacca
     1981 ttgcggtaga ccgatgtaaa tcataacgcg tgaaggatct cttccacgct acgcacacgg
     2041 gcgatgcgcg ggtagatatg attaatgctg ttattgtgtt gctcggcgct ggcggcacta
     2101 caggcgtctt cggcaatcac cagattaaag ccaagttccc aggcattgcg ggcggtggat
     2161 tcaacaccga tattggtcga gatcccacat aacactattg tatcgatacc tcggcggcgc
     2221 agttgcaact ccagatccgt accgtaaaac gcaccccact gacgtttgat gatttcgata
     2281 tcactgtcag ttgcacctaa tgcagctgga tgttgccacc agttttcggg caacacttta
     2341 gcggggg
//
LOCUS       DAJTNV010000217         2283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTNV010000217 DAJTNV010000000
VERSION     DAJTNV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.220"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(485..877)
                     /locus_tag="OO199_004034"
     CDS             complement(485..877)
                     /locus_tag="OO199_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537864.1"
                     /translation="MARPVQSHDNTEVRQYLIDKLQMDYGFRYELPGWLDDVLTGAGR
                     LNGSLGSNTKPVSKYVILTQLMSHDVITVEAVRTSISRKKMALEGKMISERYARHVAS
                     VVAAASKSIEFYKLRSKTHWKDEIALAA"
     gene            complement(1355..1918)
                     /locus_tag="OO199_004035"
     CDS             complement(1355..1918)
                     /locus_tag="OO199_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5537865.1"
                     /translation="MRFGYVRVSSQDQNTERQEAALKPHCDEILIEMKSGKDIKRNVL
                     ESLLARLRAGDELVVKSIDRLARNTKDLLTLMDELVGRGVTVRFLDNAMSFDNSPTSR
                     FMLTMMGAVAELERGFINQRQREGIAIAKEKGVYQGKPKNAELRKTLAKLFARGMSAE
                     EAAKVAKCGRATAFRIKKELSEASSGK"
BASE COUNT          560 a          577 c          567 g          579 t
ORIGIN      
        1 aaaaagggcg aacccctgat gcatctattc gacacgtcag gggttctata gtaagttgct
       61 tagcgttacg gctaggcttc tcaccgattg gcgtcattga gaataaccta ggttccgcgc
      121 cgagtccaac gggaacagaa gaaccagcca ttcggactat aaattaccgt aaccagtgag
      181 cgcccttgat gcggggtcct tagtgataaa caggcaagaa gtggtgacgt aacttggccg
      241 cgaaagagtc aagctctcac cgggaaccag accgcccgag ttaaagggtt tacctgataa
      301 gctccagatt aagacgccaa ccacaaggat gcctgacggc ctgtctctag gtcaccacta
      361 cggcatccaa ggacccttat tgccgtaaat tcactctccg tatgcttgct ggtggccccc
      421 tctggtgaat cccgaggggc aatctccttg ctggtcatcc agatttcaca cagcgcgttc
      481 atacctaagc agccagtgct atttcatcct tccaatgtgt cttacttctg agcttgtaga
      541 actcgatact cttgcttgcc gctgcgacca ccgaagctac gtgtcgggca tacctttctg
      601 aaatcatctt gccttcaagg gccatcttct tgcggcttat ggaagtcctg acggcttcca
      661 ctgtgatgac atcatgggac atcaactggg tcaggataac gtacttggat accggcttag
      721 tgttgctgcc aaggcttcca ttgaggcgtc cagcgcctgt gagtacatca tcgagccagc
      781 ccggaagctc atagcggaag ccgtagtcca tttggagttt atcgattagg tactgtcgta
      841 cttcggtatt gtcatgtgat tgaactggtc gtgccatcag gaacctcctt gaatatgctg
      901 tacgttgctg aatagggtgg cgaaatggca gacccagcaa gcaggcgcag ggagaccagt
      961 gattacggaa gtagatataa gggacctgta ggaacaggag aacctggagt tacggaagta
     1021 gatataagag aactcattga gcaactttgg ggggatggat gttgatgaca attacccatc
     1081 caactttgag aacaacagta ggccaatacg gttctcttaa agttattatt taagtagtaa
     1141 tatcaataat taatacaata ataagaacta actttaagtt tatctttaag tatggtcgct
     1201 tcgctccctg ctcgtcacgc tctccctata gtgagttgaa ttgcttgacc tgccgattta
     1261 aaagtaaaac ctgagttttc tcaaggtcct gaaaacggtc aacagataag caaacacaga
     1321 tgctcccagc ccgatggtaa ttggctaccg gtcatcactt cccgctggaa gcctcactga
     1381 gttccttctt gatgcggaaa gctgtcgccc ggccacactt ggcaacctta gctgcttcct
     1441 cagcggacat cccacgagca aacagcttag ccagcgtctt gcgcagctca gcgttcttcg
     1501 gtttcccttg gtagacaccc ttttctttgg caatggcaat gccttctctc tgtcgctgat
     1561 tgatgaagcc acgctccagt tcagccactg cacccatcat ggtcagcata aagcgactgg
     1621 tgggcgaatt gtcgaagctc atggcgttat cgaggaatct cacggttacc ccacggccta
     1681 ccagttcatc catgagggtc agcaggtcct tcgtgttcct ggctaacctg tcgatacttt
     1741 tgaccaccag ctcatcacca gccctcagac gcgctaggag cgattctaag acgttcctct
     1801 tgatatcctt accagacttc atctcaatca atatctcgtc acagtgtggc tttaaggccg
     1861 cttcctgacg ctctgtgttc tggtcctgcg aggacacgcg gacataaccg aatcgcatga
     1921 ggtattttcc tgcttacttg tatcattaag gtggctctca agatacagtc tcaataggct
     1981 cagtgtcaac gtcataatga tactcataaa cgtggagcct ccttggtatc tttggagtgt
     2041 actcaatgga tactgagctg gtgggccaat ggcaggccgg atgccgtacg cacagaaacg
     2101 cagtcagatg ttctctgcga ggctgagggg tatcaataat agggatgtac gggacccgcc
     2161 aagataacgc gacaggggcc ttctggtgaa gcctcagagg gtcgctgggg gaacctgagc
     2221 ggagttaatt agtgatatga ggtcagacaa atttgtggtg gatttctaaa ggctccggga
     2281 ccc
//
LOCUS       DAJTNV010000218         2233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTNV010000218 DAJTNV010000000
VERSION     DAJTNV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.221"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            53..2233
                     /locus_tag="OO199_004036"
                     /pseudo
     CDS             53..2233
                     /locus_tag="OO199_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          598 a          497 c          562 g          576 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccgg gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaa
//
LOCUS       DAJTNV010000219         2189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNV010000219 DAJTNV010000000
VERSION     DAJTNV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..700
                     /locus_tag="OO199_004037"
     CDS             <1..700
                     /locus_tag="OO199_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537866.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            702..1307
                     /locus_tag="OO199_004038"
     CDS             702..1307
                     /locus_tag="OO199_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5537867.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1294..>2189
                     /locus_tag="OO199_004039"
     CDS             1294..>2189
                     /locus_tag="OO199_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537868.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          508 c          611 g          538 t
ORIGIN      
        1 cgcccgtgcc ggtgtgccgg gcagcgatga ggaaacccgt gcggactttg aaaaccgccg
       61 acgtaattca gttgcccgta atgcccggaa tattctggaa gccatccggg gtgaaatact
      121 ctccacggta gaaaacgtgg tggatgttta cgtcacccat aatccgaaaa aaacagaaca
      181 aaaagccgga gtcagtcagt atccattaac acccggttcg ctttatgttg gcgtgtacgg
      241 cggcagcccg gcagatatcg cggcggccat ctggcgtaag gctccgccgg gtattgatat
      301 gaatggcaac acaacgttca ctgttgcaga taaggagtac gatccgccgt atcctgaata
      361 cgtgatcagc tggcagacac tcaaacccgt cagtctgcat gtcagtgtga cgctgaaaaa
      421 aagtgactac ctgccttcag atattacccg acaggtacag caatccgtgc tggacgcatt
      481 taatggtaca gatggtggtc tgcgggcaag agttgcctct gttgtctctg ccgggcgcta
      541 ctatgccggc atttacaaaa ccgatccgga acatattgat attctgggcc ttactgtgag
      601 ccgtgatggt tcgtcatgga caacggctgt cactttcggg atagatgaga ttccggttct
      661 ggatgtgtcg gatatcagtg tgaaactcca ggaggcataa cgtgcagaat gtggctgcca
      721 ctgtgcttgc gcagtatgct gccagccccc gactcaatgc cctcattaac agctttaacg
      781 cagcgctttc ccccgacagt tttatcagtg atttttatgg tcttatctgg aacatcgata
      841 ccgcagaaaa gtatggtctt gatgtctggg gaaagattgt gggtgtcagt tgctggctga
      901 cggtaaagga cgattttaat tacctgggct tcagcgagtc caggatggac accccggtaa
      961 tggatgatcc ctgtccgttt aatcaggcac cgttttacaa cggaaaatcg gatacccgga
     1021 ctgttgacct gtctgatgct gtataccggc ggctgatact gatgaaagcc atgtcgaaca
     1081 ttactgactg ttccgttccg gatattaacc tgatgctgag atttatgttc ggaaaaaaac
     1141 gccgggctta tgttctgaat aatggcgggc tgaggatgag ttacgtcttt gagtccgcgc
     1201 tctcgttggc agaactggcg attatccagt cgtcgggtgc actgccatcc ccgccgggtg
     1261 tttatgtttc agtagtttta aaggagtccc gtaatgaagg ccagtgataa accccgccag
     1321 ctggcggtcc cctttgcgag taccggagat aaaaaccgta tcccggacaa ggcgacacag
     1381 cagaccagag agagcggtaa tgctgcgtat gattcaggtt ttcctccggt gaccatgaca
     1441 gcggtctcag cgggaggtat accgccacac ggcaaggatt ttaacggtct gatgtacgat
     1501 attaccgcag caatacggtt cgcccaggct ggcggtttgt acacgtataa tgccggtttt
     1561 gcgggggcca ttggtggata tgcaaaagga gccattctcg ccggagtcgc aacaacagcg
     1621 gtctggctga ataccacgga cgataacctg accgatcctg aaggctccga cagtgcgggc
     1681 tgggtaaatc ttcttgagga tccgaaaagg atattcctgc ggcagaagaa caatctgtca
     1741 gaccttcaga ataaagggac ggcacgggat aatcttcagg tttacagtaa agagcagtca
     1801 gatcaacgct atgttcatcg ggaaggcgat aaaataaccg gagagctgaa aatccgtggt
     1861 gttaatgcgc tgaggatttt caacgaagct tttggcctga tttttcgtcg ttcggaagag
     1921 tgcctgcacc ttattcccac cagtgaaggt cagggggaaa atggcgatat tggtcccctg
     1981 cgcccgttca ccattaattt gcggacgggt gaaatatcca tgtcgcataa agtgtctgtt
     2041 ggcggtggtt cgcaggtcaa tggtgcgctg ggtatcggcg ttcagaacgc cctggggggg
     2101 aattcaattg ttcttggtga taatgacacc ggatttaaac agaatggaga cggtattctg
     2161 gatgtttatg ctaatagtca gcgtgtatt
//
LOCUS       DAJTNV010000220         2037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNV010000220 DAJTNV010000000
VERSION     DAJTNV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.223"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>2037
                     /gene="sat"
                     /locus_tag="OO199_004040"
     CDS             <1..>2037
                     /gene="sat"
                     /locus_tag="OO199_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5537869.1"
                     /translation="SYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHG
                     IASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQ
                     EKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKES
                     IVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGK
                     VILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVL
                     AINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQG
                     HATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDW
                     KTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGF
                     DFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVI
                     AQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASV
                     TGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNN
                     AIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          747 a          359 c          401 g          530 t
ORIGIN      
        1 tcatacggca tgatgattta taaaaatgat aaaacatttc gtaatcttga aatattcgga
       61 gacagcggct ctggagcata cttatatgat aacaaactag aaaaatgggt attagtcgga
      121 acaacccatg gtattgccag cgttaatggt gaccaactga catggataac aaaatacaat
      181 gataaactgg ttagtgagtt aaaagatacc tatagtcata aaataaatct gaatggcaat
      241 aatgtaacca ttaaaaacac agatataaca ttacaccaaa acaatgcaga taccactggt
      301 actcaagaaa aaataactaa agacaaagat attgtgttca caaatggggg aaatgttctg
      361 tttaaggata atttggattt tggtagcggt ggtattatct ttgacgaagg ccatgaatat
      421 aacataaacg gtcagggatt tacatttaaa ggagcaggaa ttgatatcgg aaaagaaagc
      481 attgtaaact ggaatgcatt gtattccagt gatgatgttt tacacaaaat aggccccggt
      541 actctgaatg ttcaaaaaaa acagggggca aatataaaga taggtgaagg aaatgttatt
      601 cttaatgaag aaggaacatt taacaatata taccttgcaa gcggaaatgg taaggtaata
      661 ctaaataaag ataattccct tggcaatgat caatatgcgg ggatattttt tactaaacgt
      721 ggtggcacgc tagatttaaa tggacacaat cagactttta ctagaattgc cgccactgac
      781 gatggaacaa caataactaa ctcagataca acgaaagaag ccgttctggc aatcaataac
      841 gaagactcct acatatatca tgggaacata aatggcaata taaaactaac gcacaatatt
      901 aattctcagg ataagaaaac taatgcaaaa ttaattctgg atggtagtgt caacacaaaa
      961 aatgatgttg aagtcagtaa tgccagtctt accatgcaag gccatgcaac agagcatgca
     1021 atattcagaa gcacagcgag tcattgctcc ctggtatttc tttgtggaac ggactgggtc
     1081 accgttttga aagaaacaga gagttcatat aataaaaagt tcaattctga ttacaaaagt
     1141 aataatcagc agacctcatt tgatcagcct gactggaaaa ccggggtgtt taaatttgat
     1201 acattacacc tgaacaatgc tgacttttca atatcacgca atgccaatgt tgaaggaaat
     1261 atatcagcaa ataaatcagc tatcacaatc ggcgataaaa atgcttacat tgataatctt
     1321 gcagggaaaa atattactaa taatggtttt gacttcaaac aaactatcag tactaatcta
     1381 tccataggag aaactaaatt tacaggtggc atcactgcac ataacagcca aatagccata
     1441 ggtgatcaag ctgtagttac acttaatggt gcaacctttc tgaataatac tcctataagt
     1501 atagataaag gagcaaaagt tatagcacaa aattccatgt tcacaacaaa aggtattgat
     1561 atctccggtg aactgactat gatgggaatc cctgaacaga atagtaaaac tgtaacgccg
     1621 ggtctccact acgctgctga tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc
     1681 agaaatatgg catctgtcac cggaaatatt tatgctgatg atgcagcaac cattactctg
     1741 ggacagcctg aaactgaaac accgactata tcgtctgctt atcaggcatg ggcagagact
     1801 cttttgtatg gctttgatac cgcctatcga ggcgcaataa cagcccccaa agctacagtt
     1861 agcatgaata atgcgatctg gcatctaaat agccagtcat caattaatcg tctagaaaca
     1921 aaagacagta tggtgcgttt tactggtgac aatgggaagt ttacaaccct tacagtggac
     1981 aaccttacta tagatgacag tgcatttgtg ctgcgtgcaa atctggccca agcagat
//
LOCUS       DAJTNV010000221         2036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNV010000221 DAJTNV010000000
VERSION     DAJTNV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..363)
                     /locus_tag="OO199_004041"
     CDS             complement(<1..363)
                     /locus_tag="OO199_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537870.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OO199_004042"
     CDS             complement(430..1644)
                     /locus_tag="OO199_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5537871.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          414 g          495 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 ggggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaatgc
//
LOCUS       DAJTNV010000222         1989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNV010000222 DAJTNV010000000
VERSION     DAJTNV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..570
                     /locus_tag="OO199_004043"
     CDS             <1..570
                     /locus_tag="OO199_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5537872.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="OO199_004044"
     CDS             571..936
                     /locus_tag="OO199_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5537873.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="OO199_004045"
     CDS             933..1616
                     /locus_tag="OO199_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5537874.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="OO199_004046"
     tRNA            1815..1890
                     /locus_tag="OO199_004046"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="OO199_004047"
     tRNA            1892..1968
                     /locus_tag="OO199_004047"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
BASE COUNT          542 a          436 c          554 g          457 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtagg
//
LOCUS       DAJTNV010000223         1962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNV010000223 DAJTNV010000000
VERSION     DAJTNV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(1..834)
                     /locus_tag="OO199_004048"
                     /pseudo
     CDS             complement(1..834)
                     /locus_tag="OO199_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1311..1547
                     /locus_tag="OO199_004049"
     CDS             1311..1547
                     /locus_tag="OO199_004049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537875.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          528 a          460 c          454 g          520 t
ORIGIN      
        1 tgctctacgg tcatattttt aattgcgctc cggtttacaa gagcccaccc ctgtttttcc
       61 agataaaact ggaaagtatc cagggtacag accagtgcac cgtcaggaac agtttcggtg
      121 aatctgatat tgccgtgttc gtcgaagtgc acaaccagag tgcgaccgtt acccggaatc
      181 atcttgtcag cggacggggt attattctgg tgcagttctg cctccatgcg gtcgaacatc
      241 agcgatgtag gcctctttga atgcagcggc ttttttccca gtgaagccca tcaccaggaa
      301 aacgaagccg tttttggtga tttggtacat tggacgtttt tcgcctttgg tgtcggtgta
      361 ggtgacgtcc tcaaaattga gggcgttaaa ttcaggtgag cattcagtat ttgcgatggc
      421 acgtaacaca ttgtcgtgtc gtttgtggaa gaactctgca accgcaacag acgtagtgac
      481 agcgcgaccg ttttcgatgg ttacgtcagg gtgagaaagg gcagggatag tagccatgat
      541 ggcatcctcg agtgataagt taattaactc accaccgagg ttttccacga ccataagggt
      601 ggtgagacgt acaggggtgg aaataccggt cactcgagaa cccggccagt cttgcgactg
      661 ccctgcacat cccaccataa tttgaatgga gctgtgcatt acgcataaaa aaaccgcttc
      721 agcgcggcta tgcgctcgag taacgttcgg gtttccacgc ccggcccccg ttttatgagg
      781 tgcagatgca ctataattcc acccgttctg gttttcaata gctacattca acattttctc
      841 ttacctttca tcaccgaagt gaactttgtt gatgcggtgc ctggtgcctc caggtgacgt
      901 taaccagtta acaattaacg ccggatacag ggaaacccat aaccacccga tacgtgggaa
      961 ctaatcgctt tttaactgtt ccgcgtgcgc tcagccgcat tcaccgcatc acaaaattca
     1021 ctttaaaaag ggcggacatc agccagcaat gaaactgatg ccgccaaagg taccaatcaa
     1081 catggagtgt tgtggcgtgg ttgtcactta agcgtatggt caacctgaca acccggtgtc
     1141 ctcaacgggg aaggaataac tccgccatac ttaccgccgc gtcatttcgc gttgtgtgcc
     1201 tgcttttaac tacgtcaggc gaggtggttc ctgttattcc ccaacaacaa gaaatttgta
     1261 taatctgaat accccaacaa taagaagagt gagcaagtga tgtattagcg atgtcagctc
     1321 tcaaagagac tgcatcaatg ttaaaattat tcaatgaagc aaaaactcag gctgaggttg
     1381 atcgcgctgt gtctgaaata aactctaagt tgtcaagcat tcagcttgaa tatttgtctc
     1441 tcattgatga gctgacaatc tctaaaagca atgaggctgc gctcaaagca aaactcacgg
     1501 agatagaaaa gtttaagttc caaagtgaag actgcgaacc tcgctaattg attactggaa
     1561 cgattgtgta tgccagacag tgcaatacta gctctggcaa gattaccatg tatttttgtc
     1621 cagaatgttt tgcgaagaga gcaatatcta tattgcaacc tttccctatc gaggaaggcg
     1681 ataaatatac gaaaactcaa tgcccctcct gtaaaaatga gtatctcatg gactacaatt
     1741 tcaataatga tttttgagtc aaggtattca gatttttaaa gaacatgccg gatactcacc
     1801 cgtgtccggc gcacgcactc cacctcaccc gtggagaact ccttaattac caaccttggc
     1861 ttcgttggtt agccattaac gctggtatgt aatcattctg gcaatgctta atttgaagtg
     1921 gcacactgaa tttggccacc tgaacagagg tgatatgctc ac
//
LOCUS       DAJTNV010000224         1936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNV010000224 DAJTNV010000000
VERSION     DAJTNV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO199_004050"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO199_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5537876.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1936
                     /locus_tag="OO199_004051"
     CDS             1494..>1936
                     /locus_tag="OO199_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5537877.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          552 a          337 c          466 g          581 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatga
//
LOCUS       DAJTNV010000225         1906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNV010000225 DAJTNV010000000
VERSION     DAJTNV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(56..1705)
                     /locus_tag="OO199_004052"
     CDS             complement(56..1705)
                     /locus_tag="OO199_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5537878.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          449 a          504 c          490 g          463 t
ORIGIN      
        1 cctgaagagg ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg
       61 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
      121 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      181 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      241 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      301 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccaaaa
      361 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      421 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      481 cgcgtcgcca gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta
      541 ccgcatcgcg acgggcactg gccttctggc ggaacagcgc ttctgtctct ttctcgcctt
      601 tcagcgccga gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga
      661 tggggatgaa ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg
      721 aagagtaatc ccaaccggag gccgctcccg cgcgtaaatc gcggtacacc tcgttgggcg
      781 ggcgaccaga atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt
      841 cgcgatcgtc ccagtaacgg ttgagcagcg atccgtccgg catccgcaca acatggcgat
      901 aggcctgatt agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttaa
      961 ggtggtcgag atagcggcgc gcaccgcgta caccatcttc ttcaaacaac tccaccgtca
     1021 gcgcaaaaac cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt
     1081 gaccgtagtt ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc
     1141 gaccactttc cgccagtccc agcatggtga aataggaatc ccaatagtag gtttcgctaa
     1201 aacggccgcc cgggacaata tatgactgcg gtagcgccag cagagaagac cacggaatgt
     1261 gatcctgtgg ttcgcgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat
     1321 tttgcgggtc cgatacatac tcgctggagt agacctccgg cagccagaag tgattttcaa
     1381 caaacttgcg caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat
     1441 ccagcgggtc cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg
     1501 ccgactgaac atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg
     1561 cgggcagccc ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta
     1621 attcatacgg gtccagttca tagcagagat cgacctcgat catcagttcg tctggattag
     1681 ggttttgaat tttctgattg agcataagga tccaacctcc gaaattcgtc gctaattatc
     1741 gggcttttct ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga
     1801 tcacgtttat taacttgtca aaaaatataa cgcactaata tcaatggaat ttataaaaat
     1861 acggatttgg tgttgttttt cattttcaga acattcaatc ctgctc
//
LOCUS       DAJTNV010000226         1874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNV010000226 DAJTNV010000000
VERSION     DAJTNV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..188
                     /locus_tag="OO199_004053"
     CDS             <1..188
                     /locus_tag="OO199_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537879.1"
                     /translation="RVRTSEPVRGNVFLHNMTAFNKLAPRTVGKREHKRGMYVYYCII
                     FCHRVILNGPARGPCGC"
     gene            complement(185..1126)
                     /locus_tag="OO199_004054"
     CDS             complement(185..1126)
                     /locus_tag="OO199_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5537880.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1138..1627)
                     /locus_tag="OO199_004055"
                     /pseudo
     CDS             complement(1138..1627)
                     /locus_tag="OO199_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1631..>1874)
                     /locus_tag="OO199_004056"
     CDS             complement(1631..>1874)
                     /locus_tag="OO199_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537881.1"
                     /translation="FKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKG
                     RNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          416 a          519 c          484 g          455 t
ORIGIN      
        1 tccgcgtgcg cacgagtgaa ccagtgcgtg gcaacgtctt cctccacaac atgacggcct
       61 ttaacaaact cgcgccgaga accgtcggga agcgtgagca caaacggggt atgtacgtgt
      121 attactgcat cattttttgc catcgggtca tccttaatgg ccccgccagg gggccatgtg
      181 gctgttaaat gccatcaacg tacgaaatgg tttctttgta cactggctcg actgcaccca
      241 gcttgccgta gtaagtgacg atctgataca gaccgcgata ctgcaccggc acgctctgaa
      301 gcggaaccag cgggtagcgg acgtattttt tatcgttggt gtacgcaacc atgcgatcct
      361 tattccccac accacggcct ttcagccatt taaccgcgcg gatattcagc ggaacaccgt
      421 tctggtgata gctgatggtg ttggtctgaa ggttcgtcaa cagggactgg ttacctgcag
      481 atgaaacgat gatgctggac aacagagcaa actgctcagg cgggatcagc aaatcacgcg
      541 gaaccacaga gtaaccagaa gcggcccacg catcagacag cacctggtta atgcttgcgc
      601 ggatttcgtc cggtgttgag gttgcccacg ttttggcagc gttgttgaca ggcacgccgt
      661 ccagggtaac aaggcctttc aggtttaatg cggaatcgcc aacatatacc tgttcatcgt
      721 tatccatctg ccatttcagt tgcataccgt catacttctg cgtatcaatc gggcggccga
      781 cctgctgagc agcctgcaat tctatgaccg tccagccaag ttccatcccc cacaggttca
      841 gcgggttacc ggatttgccg atatccacgt tcacgccagc aatagcggtt gagtctttgc
      901 ctacccagtt ttttccattc ggatttgcgc cagtacccgc agcggcgaag ctggtattcg
      961 tccagctgga aatgtcatct gcgatggagg cgtcttcacg cagttgaata tcgcggctcc
     1021 aggtgtaccc caccagtggc aggttcagcg tctggtcgag tctctccagc tccccgatga
     1081 gaaaggcacc agagctgtca acggttgcct gatcaaaagt aatcattcgt ctgttcctta
     1141 aatcttccag gaaatttctg cattgccgtc agcatcaccg gcacctgtga attcagcgtt
     1201 ggtcagcacc acgtttttgc cactgactga cgtggacatg aatccaccca gaggcacttt
     1261 gatggattca tcagtggaga tcgccttttt tgatagtgct ggcatcaaaa tcagaaccga
     1321 gattaacggt cacgtagcca cgcttcatgg cgtcgcccgg gaagttcttg cctgttccca
     1381 cctggcgaac catgtccggc tgcgacgtgg tcggataagg gcgcacgtag atccccttca
     1441 ccttgtctgc ggtatcacca tctgccagcg gcacgaaaaa accgtcatca tcgtatttac
     1501 cagccaggcc ataggcagcg aaggcgttat cggatttaag gaccaccggt tcgacggtta
     1561 agtcctgcgg gcgagagaca gccccggcaa tgccaacagg catccggtac agaaatacat
     1621 tattcatttt ttacccttta cggtttgccc agaattcagc gttttgtttg ttcagggaag
     1681 cgatactggt catgcccatg tttaggcgct gtgcatcgcc ggtggtggcg cgggtgtttc
     1741 gccctttggc aacctcagac acggcattaa acgccatgtc gaccgattgt ttcggtaatt
     1801 tgcggatatc cgcatcaccg actatctggc gaaccagcgt tttgtcagca gaagccagaa
     1861 cctcgcgttt gaac
//
LOCUS       DAJTNV010000227         1842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNV010000227 DAJTNV010000000
VERSION     DAJTNV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..195
                     /locus_tag="OO199_004057"
     CDS             1..195
                     /locus_tag="OO199_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537882.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(994..1330)
                     /locus_tag="OO199_004058"
                     /pseudo
     CDS             complement(994..1330)
                     /locus_tag="OO199_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          590 a          370 c          374 g          508 t
ORIGIN      
        1 atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt
       61 gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa
      121 gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc
      181 aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat
      241 cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct ttgtttgccg
      301 gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat
      361 aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat
      421 atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg
      481 acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac
      541 aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt
      601 acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa
      661 aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca
      721 gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc aggctatagg
      781 caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac ggtctgaata
      841 acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc gagcgctgat
      901 tctggcgaac aactgaacta atacatcaga atatgcatta tgttaaataa atatgaaaag
      961 atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc gtttaatatt
     1021 cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt acgaccacag
     1081 tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata agcagttgct
     1141 tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg atttccacat
     1201 tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc tgaagttctt
     1261 tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa cccactgtac
     1321 tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact ccatacagga
     1381 taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat ttctgacgaa
     1441 atcaacatga aaataaccat atatgataat tattataata acggctttaa ttggaataca
     1501 tatattacaa cgtattatat ataattggta ttctgggaac tatattctca aaatatagta
     1561 gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga attgataaaa
     1621 aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa atgtgatgcc
     1681 tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt cacaaaaata
     1741 attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc tccgggtaga
     1801 ggttataaat aaaaattgaa tcacgacaaa cacaatattc at
//
LOCUS       DAJTNV010000228         1794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNV010000228 DAJTNV010000000
VERSION     DAJTNV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(110..577)
                     /locus_tag="OO199_004059"
     CDS             complement(110..577)
                     /locus_tag="OO199_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5537883.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO199_004060"
     CDS             complement(643..882)
                     /locus_tag="OO199_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5537884.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO199_004061"
     CDS             complement(1017..1622)
                     /locus_tag="OO199_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5537885.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(1737..>1794)
                     /locus_tag="OO199_004062"
     CDS             complement(1737..>1794)
                     /locus_tag="OO199_004062"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537886.1"
                     /translation="VELHDKVIGHYLNIKHYQ"
BASE COUNT          569 a          397 c          317 g          511 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaatt aggtaatgac tccaacttac
     1741 tgatagtgtt ttatgttcag ataatgcccg atgaccttgt catgcagctc cacc
//
LOCUS       DAJTNV010000229         1733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTNV010000229 DAJTNV010000000
VERSION     DAJTNV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.232"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            163..1188
                     /locus_tag="OO199_004063"
     CDS             163..1188
                     /locus_tag="OO199_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5537887.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1256..1733
                     /locus_tag="OO199_004064"
                     /pseudo
     CDS             1256..1733
                     /locus_tag="OO199_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          407 a          402 c          443 g          481 t
ORIGIN      
        1 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
       61 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      121 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      181 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      241 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      301 aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga tgtttgtcgt
      361 ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc actcacagca
      421 aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg taatacgacc
      481 tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac ttattacgac
      541 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
      601 gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga ctatttcaag
      661 gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc agcgaaggca
      721 ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta tgccttgcgc
      781 atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc taaagaaccg
      841 cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg ctggacgctt
      901 ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga tgaaaaagcg
      961 gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga ttacggcaag
     1021 gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg taactactcg
     1081 gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg tgggcaaaat
     1141 aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt cggtgtgagg
     1201 taacgagcgt cgctgcggcg gcgctctctt cagcgttatg ctttccgagg tttctatgtc
     1261 tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg ttcgtgcctg
     1321 gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt tatttcaaaa
     1381 cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc tggatcgcgg
     1441 cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca cctacggacg
     1501 cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta ttgtgttcgc
     1561 ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc tgctacgaaa
     1621 gctttccaca atttatattg agatcttccg taatattgca aatcttcttc tggtactttg
     1681 ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat ctg
//
LOCUS       DAJTNV010000230         1714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTNV010000230 DAJTNV010000000
VERSION     DAJTNV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.233"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..771
                     /gene="curA"
                     /locus_tag="OO199_004065"
     CDS             <1..771
                     /gene="curA"
                     /locus_tag="OO199_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5537888.1"
                     /translation="YQPGDWVLGYSGWQDYDISSGDDLVKLGDHPQNPSWSLGVLGMP
                     GFTAYMGLLDIGQPKEGETLVVAAATEPVGATVGQIGKLKGCRVVGVAGGAEKCRHAT
                     EVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKVFDAVLPLLNTSARIPVCGL
                     VSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK
                     IHYREDITDGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            969..1634
                     /gene="mcbR"
                     /locus_tag="OO199_004066"
     CDS             969..1634
                     /gene="mcbR"
                     /locus_tag="OO199_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5537889.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          489 a          357 c          445 g          423 t
ORIGIN      
        1 tatcagcctg gcgactgggt gctgggctac agtggatggc aggactatga catatccagt
       61 ggtgatgacc tggtgaaact tggcgatcat ccgcaaaatc catcgtggtc gctgggtgtg
      121 ctggggatgc caggctttac cgcttatatg gggctgctgg atatcggtca gcctaaagag
      181 ggcgaaacgt tggtggtagc tgcggcgaca gaaccagtgg gggcgacggt ggggcaaatc
      241 ggcaaactta aaggttgcag agtggtgggg gtagccggtg gcgcggaaaa atgccgccat
      301 gctaccgagg tgttaggctt cgatgtttgc cttgatcacc acgcggacga ttttgccgaa
      361 caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt gggcggtaag
      421 gtatttgatg cagtgctacc attacttaat acatctgcgc gcattcccgt ttgtggctta
      481 gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc cctgttgatg
      541 gcaacggtgc tgaaaaaacg tattcgcttg caagggttta ttatcgctca ggattatggt
      601 caccgcatcc atgagtttca gaaggagatg gggcaatggg tgaaagagga taaaatccac
      661 taccgcgaag atattactga tggtttagag aatgcgccac agacgtttat cggcctgctg
      721 aagggtaaaa acttcggcaa agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac
      781 ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt taattaacta
      841 accacgtcat tgttctgctg ctaataacaa caaaggtggg tggtttatga ttattcactt
      901 taatacacta ggtgaactcc ttctgccatg caggcagggt tggacagaaa acacgcgaag
      961 gaattaaaat gcctggaacg ggaaaaatga aacatgtcag tttgactctg caggttgaga
     1021 acgacctgaa acatcagctt agtattggcg cattaaaacc tggcgcacgc ctgattacta
     1081 aaaatctggc ggagcaatta ggtatgagta ttacacctgt gcgtgaagca ttattacgtc
     1141 tggtttcggt gaatgcgctt tctgtcgcac ctgcacaagc atttacagta ccggaagtga
     1201 ggcaaagtca gttagacgaa atcaatcgga tccgctacga actggaatta atggcagttg
     1261 ccctggctgt tgaaaacctc tcctcgcaag acatagcaga acttcaggaa ttgctcggga
     1321 aattacagca ggcgcaagaa aagggcgaca tggaacaaat cattaatgtg aacaggctat
     1381 ttcgcttagc gatttatcat cgctcaaata tgctcatcct gtgtgagatg attgagcaac
     1441 tgtggatcag gatgggacct gctttacatt atctttatga agcgattaac ccagcggaat
     1501 tacgggagcg catagaaaac tatcatctat tactcgctgc gttaaaagca aaagacaaag
     1561 agggatgcaa acattgtctt gctgaaatta tgcaacaaaa tattgctatt ttataccagc
     1621 aatacaatcg ttaaattaaa tgcccggcaa ataccgggca tcaagtaatt actcaaatcg
     1681 ccacgcaata ttcatgccga caccataatt tcgt
//
LOCUS       DAJTNV010000231         1645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTNV010000231 DAJTNV010000000
VERSION     DAJTNV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.234"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..545)
                     /locus_tag="OO199_004067"
     CDS             complement(<1..545)
                     /locus_tag="OO199_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537890.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1200..>1645)
                     /locus_tag="OO199_004068"
     CDS             complement(1200..>1645)
                     /locus_tag="OO199_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537891.1"
                     /translation="KPSDQAEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAA
                     QRAGHEGISELLGCTVCGYAGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTA
                     GYDLELISVVDVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          474 a          365 c          343 g          463 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggaacctagg tatcgagggt atcgaagata atcagccgct aaccatttgt ttccttaaag
      841 gatgatgaat ccttgtggtt acagttatgg ttacacatta atttatctct ggtcaagtcc
      901 cgaagtccta actttaagac agtggcccca atcgtccaga cttgaaaggg gcctatgggg
      961 gaagtcgctg acctttatct attaaatacc tacagagatt tttgtgagat tttttaatcc
     1021 aaggtcataa cctactgccc ccacaggccg caaagtgaag acctgtaagc accagagcaa
     1081 tagcgagtat ctatcactcc ctgtagagta tctggtagac caagaggaca cagaggacga
     1141 ccatatacaa ctcctgtaag ctcatggatg tactccttat gttcactcag ggtttatagt
     1201 tactctgcgg cagtaaaacg aataacatgg accaacggga aagaggcgtc ttcctcaacg
     1261 tcaaactcac ctgctttcac cttaacggaa ccagtagcca acacatcgac cacactgatt
     1321 aactcaaggt catacccagc agtgttgcta tttcgggtca ctttgacatc taccgtctta
     1381 gcgtctttag ttgacctgaa agagatagaa gcgaaccgct taggcaggag atagacacct
     1441 gtatcatcgc ccgcatagcc gcatacagtg cagcccaata gctcactaat accttcatga
     1501 cctgcgcgtt gagcggctcg gtccaagatt gcctcaagtt tctcagggac caatacagcg
     1561 taatcgctca gtgtatttcc caactcgacc ggaagattca acgctaatgt atccagaaga
     1621 tcttcagctt gatcagacgg tttac
//
LOCUS       DAJTNV010000232         1612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTNV010000232 DAJTNV010000000
VERSION     DAJTNV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.235"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..1193
                     /gene="malF"
                     /locus_tag="OO199_004069"
     CDS             <1..1193
                     /gene="malF"
                     /locus_tag="OO199_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5537892.1"
                     /translation="TYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLKET
                     TAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGTLT
                     NNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKPFL
                     AIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISILIF
                     KGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLLKA
                     IPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGP
                     DRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKATR
                     MKFD"
     gene            1208..>1612
                     /gene="malG"
                     /locus_tag="OO199_004070"
     CDS             1208..>1612
                     /gene="malG"
                     /locus_tag="OO199_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="with MalKFE is involved in the transport of maltose
                     into the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5537893.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVL"
BASE COUNT          350 a          437 c          431 g          394 t
ORIGIN      
        1 aaacctataa ctttggtctt tacccggcgg gcgatgagtg gcaactggcg ctcagcgacg
       61 gcgaaaccgg caaaaattac ctctccgacg cgtttaaatt tggcggcgag caaaaactgc
      121 aactgaaaga aacgaccgcc cagcccgaag gcgaacgcgc gaatctgcgc gtgattaccc
      181 agaatcgtca ggcgctgagt gacattaccg ccattctgcc ggatggcaac aaagtgatga
      241 tgagctccct gcgccagttt tctggcacgc agccgctcta cacactcgac ggtgacggca
      301 cgttgacgaa taatcagagt ggcgtgaaat atcgtccgaa taaccaaatt ggcttttacc
      361 agtccattac cgccgacggc aactgggggg atgaaaagct aagccccggt tacaccgtga
      421 ccaccggctg gaaaaacttt acccgcgtct ttaccgacga aggcattcag aaaccgttcc
      481 tcgccatttt cgtctggacc gtggtgttct cgctgatcac tgtcttttta acggtggcgg
      541 tcggcatggt tctggcatgt ctggtgcagt gggaagcgtt gcgcggcaaa gcggtctatc
      601 gcgtcctgct gattctgccc tacgcggtgc catcgttcat ttcaatcttg attttcaaag
      661 gattgtttaa ccagagcttc ggtgaaatca acatgatgtt gagcgcgctg tttggcgtga
      721 agcccgcctg gttcagcgat ccgaccaccg ctcgcacgat gctgattatc gtcaatacct
      781 ggctgggtta tccgtacatg atgatcctct gcatgggctt gctgaaagcg attccggacg
      841 atttgtatga agcctcagca atggatggcg caggtccgtt ccagaacttc tttaagatta
      901 cgctgccgct gctgattaaa ccgctgacgc cgctgatgat cgccagcttc gcctttaact
      961 ttaacaactt cgtgctgatt cagctgttaa ccaacggcgg cccggatcgt cttggcacga
     1021 ccacgccagc cggttatacc gacctgcttg ttaactacac ctaccgtatc gcttttgaag
     1081 gcggcggggg tcaggacttc ggtctggcgg cagcaattgc cacgctgatc ttcctgctgg
     1141 tgggtgcgct ggcgatagtg aacctgaaag ccacgcgaat gaagtttgat taagggagat
     1201 aacaaaaatg gcaatggtcc aaccgaaatc gcaaaaagca cgtttgttta ttactcacct
     1261 gctactgcta ctttttatcg cggcgattat gttcccgctg ctgatggtcg tcgctatctc
     1321 gctgcgtcag ggaaacttcg cgaccggtag cctgatcccg gagcaaatct cctgggatca
     1381 ctggaaactg gcgttaggtt ttagcgttga acaggctgat ggtcgcatta cgccaccgcc
     1441 attcccggta ctgctgtggc tgtggaactc ggtaaaggtc gctgggattt ccgcgattgg
     1501 cattgtggcg ctctccacca cctgcgccta cgctttcgcc cgtatgcgct ttccaggcaa
     1561 agcgacgctg ctgaaaggaa tgctgatttt ccagatgttc ccggcagtac tt
//
LOCUS       DAJTNV010000233         1599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNV010000233 DAJTNV010000000
VERSION     DAJTNV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            154..477
                     /locus_tag="OO199_004071"
     CDS             154..477
                     /locus_tag="OO199_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5537894.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="OO199_004072"
     CDS             474..890
                     /locus_tag="OO199_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5537895.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="OO199_004073"
     CDS             856..1380
                     /locus_tag="OO199_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537896.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1599
                     /locus_tag="OO199_004074"
     CDS             1377..>1599
                     /locus_tag="OO199_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537897.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          390 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagca
//
LOCUS       DAJTNV010000234         1593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNV010000234 DAJTNV010000000
VERSION     DAJTNV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(324..470)
                     /locus_tag="OO199_004075"
                     /pseudo
     CDS             complement(324..470)
                     /locus_tag="OO199_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            524..706
                     /locus_tag="OO199_004076"
     CDS             524..706
                     /locus_tag="OO199_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5537898.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(807..1136)
                     /locus_tag="OO199_004077"
     CDS             complement(807..1136)
                     /locus_tag="OO199_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5537899.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1308..>1593)
                     /locus_tag="OO199_004078"
     CDS             complement(1308..>1593)
                     /locus_tag="OO199_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537900.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          433 a          366 c          375 g          419 t
ORIGIN      
        1 tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt ccctgaaggc
       61 gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga acaaagctga
      121 ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg gtatccaacc
      181 cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca gggaattaaa
      241 cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt catatcagcg
      301 tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca tggcaggagt
      361 tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg cagatacgct
      421 tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag caggaatctc
      481 agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc acccggtcat
      541 tggagtagta accaaagcag atttagccag catggaacaa atatctttgg tgaaaagttg
      601 gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata acaatggagt
      661 tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc gattggttct
      721 cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga gggttgccgg
      781 gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat ttcgcccata
      841 gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt tttgatgatg
      901 taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc ctggattgct
      961 tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag gacgcgcttc
     1021 tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt gttcttacga
     1081 cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt ttccattttt
     1141 atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt tagggccaac
     1201 attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc atacccttta
     1261 tccttgctga atcgaagcag cagcaagatg attctgaagt tcagaaatta tttgcgcaag
     1321 gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat gttcagccac
     1381 acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc gagcaactga
     1441 cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg ctgcggatta
     1501 ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg ctgggtatca
     1561 caaagttttt gcgcgttcaa taacacgaaa tgg
//
LOCUS       DAJTNV010000235         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTNV010000235 DAJTNV010000000
VERSION     DAJTNV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..626)
                     /locus_tag="OO199_004079"
     CDS             complement(<1..626)
                     /locus_tag="OO199_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5537901.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO199_004080"
     CDS             complement(740..1312)
                     /locus_tag="OO199_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5537902.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          473 a          333 c          315 g          466 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtatttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTNV010000236         1538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNV010000236 DAJTNV010000000
VERSION     DAJTNV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.239"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(910..1386)
                     /locus_tag="OO199_004081"
     CDS             complement(910..1386)
                     /locus_tag="OO199_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5537903.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAJTNV010000237         1381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTNV010000237 DAJTNV010000000
VERSION     DAJTNV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.240"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..105)
                     /locus_tag="OO199_004082"
     CDS             complement(<1..105)
                     /locus_tag="OO199_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5537904.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(146..601)
                     /gene="csgB"
                     /locus_tag="OO199_004083"
     CDS             complement(146..601)
                     /gene="csgB"
                     /locus_tag="OO199_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5537905.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT          397 a          289 c          237 g          458 t
ORIGIN      
        1 gccaccaccg tggttaccgc cgccgccgta ctgaggaaca acacctgcca gagcgctacc
       61 ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg
      121 gattgattta aaagtcgaat ggtaattaac gttgtgtcac gcgaattgcc atttgcgact
      181 gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt gctttattac
      241 cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa atactggcat
      301 cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc tggtcaatct
      361 ttgcccgatt gctactacct tcttgcgcaa caaccgccaa aagttttgag cctccctgcc
      421 gtaactgagc actattatta gtcccagctt gaccaattat ggctgcctga ttaaatgaag
      481 acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca taacctgctg
      541 cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat ttgtttttca
      601 tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa cttggttgtt
      661 aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg cggaaatatt
      721 tttaataact cacccgcgcg ttgtattttt ctttttttgt tcataccaaa gtattaaaac
      781 ccgcgtaaat acgctgttat ctacgcaaaa aatattttgt tttctcttaa atctccgttt
      841 tccgctatca aaaatcacca gacagtcatt cttcttgccc gtcgctgatt gctgcgatat
      901 gtcttgcgca caagccgtga caaacctcgt ctttttgcag taaaaaaatt gtccacggag
      961 gtgtggagaa aaaacaaaaa cgttttacat gacgaaagga ctacaccgaa atatttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 g
//
LOCUS       DAJTNV010000238         1336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNV010000238 DAJTNV010000000
VERSION     DAJTNV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..199
                     /locus_tag="OO199_004084"
     CDS             <1..199
                     /locus_tag="OO199_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537906.1"
                     /translation="EMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYR
                     QLTDEVLALRLPENGSQLHHS"
     gene            complement(379..1002)
                     /locus_tag="OO199_004085"
     CDS             complement(379..1002)
                     /locus_tag="OO199_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5537907.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(953..>1336)
                     /locus_tag="OO199_004086"
     CDS             complement(953..>1336)
                     /locus_tag="OO199_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537908.1"
                     /translation="YWPISRLTDWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIA
                     AYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPG
                     SYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          293 a          391 c          332 g          320 t
ORIGIN      
        1 tgagatgctg gagaatgagt accctcagag ggtggctgac cggctgaaag catcaggtct
       61 gagcggtgat gcggatgcgg agagggaagc cggtgcacag gtgatgcgtg agactgaaca
      121 gcagatttac cgtcagctga ctgacgaggt actggccctg cgattgcctg aaaacggctc
      181 acaactgcac cattcataat cacatcgcat aaaccacaga ccggactgac tccggaaaaa
      241 cagaggcccg cccccgggcc tccccggatt catccgtttc tctgttcagc ctgaccgcac
      301 accccggcgg ccggatgaca gactccgcct cggtaagcaa agcggtcttc tgtgattccg
      361 ccagttgcgg cttattcatt acttaacgtc aaacgcccga attgaagcca aatcatccag
      421 actgctcagc tcctctttca tctcccgctg acggcgataa atctcatcgt tgcgatcgac
      481 ctgcgcctgc accattgctg ccgccagttc ttccagttcc ggcatcgaca gtttcacctg
      541 ctgattatcc gcatcactcc acaccatatg tgtttgtgcc gcgacagatt ttgccagcat
      601 gaccaccggg gacaggcggg caactgacga ggcgtcagcg ttccacgtgc ggccgttcca
      661 ttcaaacgtg aacggcttcg cctcctgttc tgtgcgccat gcttcaattt cctgacgtct
      721 ggcctctctg gccgcttcca gcatttctgg tgtcacagtg aatggggcta tctcacccca
      781 tttgccactt tgcagttcct gccagatttg ctgacccgtc ggtgcaacat catcagcggt
      841 ggctgtgtag ggaactgcct ggtccctgtc gtcaaaaaaa acgtcacagt ctactgcgcc
      901 actttcggta taacggggat taatgatttt tttaatttcc acggtgcatt cctcacgatg
      961 tgcgaataaa aagcccgggc attgcgccag agacatgagc atccggcacc ccggacaggg
     1021 cgcaatatga ccctggtaat gaatgctctg aacatcccgt aatgaaaaat tgtggggatg
     1081 ctatatacgt tccggtggga gtactgggca ctgaaatccc caccggtccc agtcgtgagc
     1141 ctctgtatga ctgccccctg acaagtctga tgactttatc accgtcagct tctccctggt
     1201 acgcagcaat aatcagcccg ccaatgtcag ggtctcccca tctgttgcgg acagagctcg
     1261 ccacgattct gtaaataata tcttctgtgg ttatatttat tttcacccag tcagtcagtc
     1321 tggatatggg ccagta
//
LOCUS       DAJTNV010000239         1280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNV010000239 DAJTNV010000000
VERSION     DAJTNV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(41..100)
                     /locus_tag="OO199_004087"
     CDS             complement(41..100)
                     /locus_tag="OO199_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5537909.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="OO199_004088"
     CDS             complement(339..1247)
                     /locus_tag="OO199_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537910.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          254 a          345 c          311 g          370 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac
//
LOCUS       DAJTNV010000240         1259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNV010000240 DAJTNV010000000
VERSION     DAJTNV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            34..435
                     /locus_tag="OO199_004089"
     CDS             34..435
                     /locus_tag="OO199_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5537911.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO199_004090"
     CDS             447..818
                     /locus_tag="OO199_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537912.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="OO199_004091"
     CDS             805..>1259
                     /locus_tag="OO199_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537913.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAJTNV010000241         1242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNV010000241 DAJTNV010000000
VERSION     DAJTNV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..758
                     /gene="malE"
                     /locus_tag="OO199_004092"
     CDS             <1..758
                     /gene="malE"
                     /locus_tag="OO199_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5537914.1"
                     /translation="DLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSTGINAASPNKELAKEF
                     LENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMS
                     AFWYAVRTAVINAASGRQTVDEALKDAQTRITK"
     gene            912..>1242
                     /gene="malF"
                     /locus_tag="OO199_004093"
     CDS             912..>1242
                     /gene="malF"
                     /locus_tag="OO199_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="with MalKGE is involved in maltose transport into
                     the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5537915.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLL"
BASE COUNT          332 a          308 c          335 g          267 t
ORIGIN      
        1 aagacctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
       61 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      121 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      181 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      241 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      301 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
      361 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatct aaaccgttcg
      421 ttggcgtgct gagcaccggt attaacgccg ccagtccgaa caaagagctg gcgaaagagt
      481 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
      541 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
      601 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
      661 tctggtatgc cgtacgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
      721 aagccctgaa agacgcgcag actcgtatca ccaagtaatg ctgtgaaatg ccggatgcgg
      781 cgtgaacgcc ttgtccggcc tacaaaactg aaacgcatgt aggcctgata agacgcatca
      841 gtgtcgcatc aggcagttgt tgtcggataa ggcgtgaaag ccttatccgt cctggaatga
      901 ggaagaaccc catggatgtc attaaaaaga aacattggtg gcaaagcgac gcgctgaaat
      961 ggtcagtgct aggtctgctc ggcctgctgg tgggttacct tgttgtttta atgtacgcac
     1021 aaggggaata cctgttcgcc attaccacgc tgatattgag ttcagcgggg ctgtatattt
     1081 tcgccaatcg taaagcctac gtctggcgct atgtttaccc gggaatggct ggaatgggat
     1141 tattcgtcct cttccctctg gtctgcacca tcgccattgc cttcaccaac tacagcagca
     1201 ctaaccagct gacttttgaa cgtgcgcagg aagtgttgtt ag
//
LOCUS       DAJTNV010000242         1238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNV010000242 DAJTNV010000000
VERSION     DAJTNV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            119..>1238
                     /locus_tag="OO199_004094"
     CDS             119..>1238
                     /locus_tag="OO199_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5537916.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQVLI"
BASE COUNT          267 a          389 c          323 g          259 t
ORIGIN      
        1 gcgctggtaa ccgctaatag gggtcaggtc aggcactttt gccgggaccg tctgtaacgt
       61 ggatgccggt acctgctccc cgtggttatc tggttaaccc atatacaagg gagacagaat
      121 gaccgaatcc agcgattacg aatccgtcca ggtctttatc ggcgttgatg tcggtaaaga
      181 tacgcatcac gctgttgcca ttaatcgttc aggtaaacgc ctgttcgata aagcattacc
      241 caacgacgaa aacaaactca ggtcgctaat atctgacctg aaacaacatg gtcagatact
      301 gctggttgtt gatcagccag ctaccatcgg tgcgttacct gtcgccgttg cccgctcaga
      361 aggagtcctt gtcggatacc tccctggact ggccatgcgc cgcatagccg acttacacgc
      421 cggtgaagct aaaactgatg ctcgtgacgc tgccatcatt gccgaagctg cccgtaccct
      481 gcctcacgcg ctacgcacgc tgaaactggc tgacgagcaa atcgccgaac tctccatgct
      541 ctgcggcttc gatgatgatc ttgccgcaca gacaacgcag gccagcaacc gtatccgcgg
      601 ccttctgacc cagatacatc cggcaccgga gcgcgttctc ggtccgagac ttgagcaccc
      661 ggcggtactc gatcttctcc agcgatatcc ctcaccagaa aaactcgctt cgctgggtga
      721 gaagaagctg gcagcccagc tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc
      781 agacatagct caggcactgg ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc
      841 cgtagtactg ccacgtctgg cactccagct catcacgctg cgtaagcaaa gagacgaggt
      901 ggcgcttgag gtagaacagc gagttcttgc tcaccctctt tacccggtcc tgaccagtat
      961 gcccggagtc ggtgtcagga ccgcagccag actcctcacc gaggtcgcct gccgcgcctt
     1021 cgcctctgcc gcacatctcg ctgcttatgc tggccttgcg ccggtaactc ggcgatccgg
     1081 ctcgtcaata cgcggtgagc atccctcgcg acggggtaat aaagctctca aacgggcgtt
     1141 gttcctgtcg gccttcgccg cgctcaggga tccgctctcc agggcttact acacccgcaa
     1201 aatgagtcag ggaaaacgac acaatcaggt gcttatcg
//
LOCUS       DAJTNV010000243         1228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNV010000243 DAJTNV010000000
VERSION     DAJTNV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            151..366
                     /locus_tag="OO199_004095"
     CDS             151..366
                     /locus_tag="OO199_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537917.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            469..747
                     /locus_tag="OO199_004096"
                     /pseudo
     CDS             469..747
                     /locus_tag="OO199_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            749..>1228
                     /locus_tag="OO199_004097"
     CDS             749..>1228
                     /locus_tag="OO199_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537918.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     "
BASE COUNT          305 a          299 c          316 g          308 t
ORIGIN      
        1 taacccgctt cggcgggttt ttgtttttat tttcaacgca tttgaagttc tggatggtgc
       61 cggaatagaa tcaaaaatac ttaagtagcg cgcagggata aaagggatgg accccgaaca
      121 ggggagtgct acttatctgg aaggattctg atgatgaaca tcgaagaact gcgtaaaatt
      181 ttttgtgaag atggccttta tgctgtgtgc gttgaaaatg gaaatattgt tagtcattac
      241 cgcattgtgt gtttgcaaaa aaatggggct gcgttaatta attttgtgga tgcccgagtg
      301 acggacggat ttatcttgcg cgacggtgag tttgtcactt cattacaggc attgaaagag
      361 atctgaataa aagctggctt ttctgctttt tcagaagaat aaactcatct acaatcttgc
      421 gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat ggcacgcaac
      481 gcaaaatatt acaatcatga taattcgacc gttcttgccc acacgcacga gcggtattct
      541 cacgcattta agtcagactg gtaccagcat ccctcatgca ctgaagaaca ggccgaatgg
      601 cttattcagt gttaccgcag gcgcggatgc gaggttaaaa aagcccttag cctcgactac
      661 cgtcactaga taatctccgt caggctccct tactccgaac agccaccgcg tccgtcccgc
      721 acattccagc aacggatctg gaggtaacgt gcgggtatta cttcgacctg ttctggttcc
      781 ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc atgccggtat tccacaatac
      841 ccgggtactg gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg gggtcgttcc
      901 tgccgttcgc cagccgctag tggaagacaa aacattgctg ccgtttttca gtaacgcacg
      961 ggtgattcgt gctgctggtg gtgctggcgc attgtctgac tggctgttgc gccatattaa
     1021 atcctgccag tggccacacg gcgattatca tcacagcgaa accgtcattc accgttatgg
     1081 taccggcgca atggtattgt gctggcactg cgacaaccag ctgcgtgacc agacatccga
     1141 atcactcgag caacttgctc atcaaaacct gtcagcatgg atgattgacg tcatcggtca
     1201 cgcaataagc ggtacgcagg agcgtgaa
//
LOCUS       DAJTNV010000244         1224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNV010000244 DAJTNV010000000
VERSION     DAJTNV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..597
                     /gene="ssuA"
                     /locus_tag="OO199_004098"
     CDS             <1..597
                     /gene="ssuA"
                     /locus_tag="OO199_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5537919.1"
                     /translation="KTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQPTYLTP
                     ADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPYAEKNG
                     AFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKPVNAEV
                     AALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            594..>1224
                     /locus_tag="OO199_004099"
     CDS             594..>1224
                     /locus_tag="OO199_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5537920.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQ"
BASE COUNT          283 a          345 c          321 g          275 t
ORIGIN      
        1 aaaaccgtag cagatcttaa aggtcacaaa gttgcctttc agaaaggttc cagttcacac
       61 aaccttttac tgcgtgcact gcgtcaggcc ggacttaaat tcaccgatat ccagcccact
      121 tacctgacgc ctgccgatgc ccgcgccgcg ttccagcaag gtaacgttga cgcctgggct
      181 atctgggatc cctactactc cgctgcgtta ttacagggcg gcgtgcgggt gctgaaagac
      241 ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtcccta tgcagaaaaa
      301 aacggcgctt ttattcaggg cgtactggca acctttagtg aggccgatgc gttaacccgc
      361 agccagcgcg aacaaagcat cgctttactg gcaaaaacga tgggcttacc ggcaccggtg
      421 attgcctctt acttagatca tcgccctcct accaccatca aaccggttaa cgccgaggtt
      481 gccgccttac agcagcaaac ggcagatctg ttttatgaaa accgtctggt gccgaaaaaa
      541 gtcgatattc gccagcgcat ctggcagccc actcaactgg aaggaaaaca attatgagtc
      601 tgaatatgtt ctggttttta ccgacccacg gtgacgggca ctatctggga acggaagaag
      661 gttcacgccc ggttgatcac ggttatctgc aacaaattgc gcaagcggcg gatcgtctgg
      721 gctataccgg tgtgctgatc ccaacggggc gatcgtgtga agatgcgtgg ctggttgcag
      781 cgtcgatgat cccggtgacg cagcggctga agtttcttgt cgccctgcgt cccagcgtaa
      841 cctcacctac tgttgccgcc cgccaggccg ccacgcttga ccgtctctca aatggtcgtg
      901 cgttgtttaa tctggtcaca ggcagcgatc cacaagagct ggcaggcgac ggcgtgttcc
      961 ttgatcatag cgagcgctac gaagcctcgg cggaatttac ccaggtctgg cggcgcttat
     1021 tgctggggga aaccgtcaat ttcaacggta aacatattca tgtgcgcgga gcaaaactgc
     1081 tcttcccgcc gattcaacag ccttatcctc cgctttactt tggcggatcg tcagatgtcg
     1141 cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa ccgccggaac
     1201 ttgttaaaga gaaaatcgaa caag
//
LOCUS       DAJTNV010000245         1205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNV010000245 DAJTNV010000000
VERSION     DAJTNV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            119..916
                     /locus_tag="OO199_004100"
                     /pseudo
     CDS             119..916
                     /locus_tag="OO199_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          359 a          213 c          288 g          345 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tgatggttta tctggtgggt taattgatgg
      901 tctgaagatg cttgctgtgg ctgttactgc tcaaaaggct gcaaatgtag caggaggctt
      961 gttaaacctt gcaaacgctg ttacttcatt cggtgacgct gttaataaag ctaaagctgg
     1021 caaggctggt tcttcaggtg gtgcgctaaa taaagtcggt agagttgtcc tttatgctga
     1081 gttggtaaat tttgctcttg ataatttaaa ccctgaagca gctaagaatc cgaacttaca
     1141 tcaatcaggt attggcacag aaacaaactt caaccctgaa tacaaaggtg attcatctaa
     1201 gccaa
//
LOCUS       DAJTNV010000246         1156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNV010000246 DAJTNV010000000
VERSION     DAJTNV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.249"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..402)
                     /locus_tag="OO199_004101"
     CDS             complement(<1..402)
                     /locus_tag="OO199_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCR5537921.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OO199_004102"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OO199_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5537922.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
BASE COUNT          292 a          304 c          261 g          299 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgc
//
LOCUS       DAJTNV010000247         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNV010000247 DAJTNV010000000
VERSION     DAJTNV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            105..>1148
                     /gene="cadB"
                     /locus_tag="OO199_004103"
     CDS             105..>1148
                     /gene="cadB"
                     /locus_tag="OO199_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR5537923.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          242 a          255 c          296 g          355 t
ORIGIN      
        1 atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat agttatacat
       61 catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga
      121 agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg
      181 cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct
      241 ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc
      301 aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt tttcagacag
      361 gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt accgctgtat
      421 cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta
      481 ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg gtaagccgtt
      541 taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct attgttggct
      601 ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg
      661 gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt gttgaatccg
      721 cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc
      781 tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt tccggtatgt
      841 atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct tcagctatcc
      901 tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc
      961 tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact
     1021 tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgctgctgg
     1081 ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct gccggtggta
     1141 aagcatct
//
LOCUS       DAJTNV010000248         1119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTNV010000248 DAJTNV010000000
VERSION     DAJTNV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.251"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..177
                     /gene="kil"
                     /locus_tag="OO199_004104"
     CDS             <1..177
                     /gene="kil"
                     /locus_tag="OO199_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5537924.1"
                     /translation="VTPGDYVLHEGRTYIASANNIKKRKLYIRSLTTKTCISDCMIKV
                     FLGRDGLPVKAESW"
     gene            171..347
                     /locus_tag="OO199_004105"
     CDS             171..347
                     /locus_tag="OO199_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537925.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            422..697
                     /locus_tag="OO199_004106"
     CDS             422..697
                     /locus_tag="OO199_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537926.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            797..>1119
                     /locus_tag="OO199_004107"
     CDS             797..>1119
                     /locus_tag="OO199_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5537927.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQD"
BASE COUNT          386 a          212 c          236 g          285 t
ORIGIN      
        1 gtgactcctg gcgattatgt tcttcatgaa ggtcggacat atatcgcctc ggcaaacaat
       61 attaaaaagc gaaaacttta tattcgtagc ctgactacaa aaacatgcat ttctgactgc
      121 atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa aggcggagtc atggtaatga
      181 ctaagaaaat aaaatgtgct tatcaccttt gcaataaaga aattgaagaa agcaaaatca
      241 ttacaagacc acttcatttc atgcgtggag ttataccaac gacggaaatg aaaaaatatt
      301 gtagtgaaat ctgtgccgaa aaagaccaga tggcacacga actttaatta actgactatc
      361 cgaaactgaa tttatgccag caatggcagg gattcgctca accttaatta aggagaaaaa
      421 catgattacc agttatgaag ccactgttgt aactactgat gacattgttc acgaggttaa
      481 tctggaagga aagcgtattg gctacgtgat taagacagaa aataaagaaa ccccattcac
      541 tgtggttgat atcgacggtc catcaggcaa cgttaaaaca cttaacgatg gtgtaaaaaa
      601 aatgtgtctg gtgcacatag gaaagaatct gcccgcagaa aaaaaagccg aatttctggc
      661 aactctgatt gcaatgaaat taaaaggtga aatctgaaaa aaagaaagcc tgcacactgt
      721 gcaggcctga atgaagaacc tgggacattt attcatcact cgcagtaatt ttaatctgag
      781 ttgaggttaa aaaacaatga acaccgataa acaagtttac ccactgtatt acgaagcaaa
      841 aaatgacaaa gtaagaaaac gtctcggtat taaaggcggt ttttactggg ctgaagcgaa
      901 aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgcag gctacgatga
      961 agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttgatgacc tcccgccaga
     1021 aggcgtattt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag atggcatcac
     1081 aatggtattt atcgcatctt ctccctctgt tcaggacaa
//
LOCUS       DAJTNV010000249         1108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNV010000249 DAJTNV010000000
VERSION     DAJTNV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            329..>1108
                     /gene="uacT"
                     /locus_tag="OO199_004108"
     CDS             329..>1108
                     /gene="uacT"
                     /locus_tag="OO199_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5537928.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTM"
BASE COUNT          250 a          258 c          250 g          350 t
ORIGIN      
        1 tcaaattatt gcccctcaat ctatgccgct ttcattattt caacattgcc gatcaaaaga
       61 gcgctatcca gataaaaacg attaaatgcg agagtgcgac atgccagaat gattaatgaa
      121 atatcaatat gagaaaatta cagataattc cattgtcagg ttctcttgtc attttcgtgg
      181 tttaagcacg attatgtgat tgcctccgca attcctgtct ctaactcccc ttcctcgcaa
      241 aaactggcac tccacgagca tgtgtttaga cagtttcatt aacgtaaacg gttgcttttt
      301 attctggcgg gcgaaaggag aaacactgat gagcgccata gattcccaac ttccctcatc
      361 ttctgggcaa gaccgcccaa ctgatgaggt tgaccgcata ttatcaccag gaaagctgat
      421 catactcggt ctgcaacacg tccttgtcat gtacgcaggt gcagtcgctg ttcctcttat
      481 gattggtgac cgactgggcc tctcaaaaga tgctattgcg atgctcatta gctcggatct
      541 cttttgctgc gggatcgtca cattattgca atgtatcggt atcggccgct ttatggggat
      601 ccgcctgccg gtgattatgt cggtgacctt tgctgctgta acaccaatga tagccattgg
      661 gatgaacccg gatatcggcc tgctggggat atttggtgcc actatcgccg cgggttttat
      721 caccacatta ttagcgccac ttattggtcg cttgatgcct ttattcccgc cactggttac
      781 cggtgtggtt attacttcta ttgggcttag catcattcag gtgggtattg actgggccgc
      841 tggaggtaaa gggaatccgc aatatggtaa tcccgtttat ttaggtatct cctttgccgt
      901 cttaattttt atcttgctca ttactcgcta tgcgaaagga ttaatgtcca acgtcgccgt
      961 attactgggg attgtatttg gctttttact ttcgtggatg atgaatgaag tcaatttatc
     1021 cgggctacat gatgcctcgt ggtttgcgat tgtcaccccg atgtcgtttg gtatgccgat
     1081 tttcgatccc gtttccattc tgaccatg
//
LOCUS       DAJTNV010000250         1101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNV010000250 DAJTNV010000000
VERSION     DAJTNV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..1019
                     /locus_tag="OO199_004109"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO199_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          310 a          270 c          256 g          265 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc a
//
LOCUS       DAJTNV010000251         1082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNV010000251 DAJTNV010000000
VERSION     DAJTNV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..395
                     /locus_tag="OO199_004110"
     CDS             <1..395
                     /locus_tag="OO199_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537929.1"
                     /translation="AAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVI
                     PLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGR
                     NSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            398..943
                     /locus_tag="OO199_004111"
     CDS             398..943
                     /locus_tag="OO199_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5537930.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            967..>1082
                     /locus_tag="OO199_004112"
     CDS             967..>1082
                     /locus_tag="OO199_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5537931.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVA"
BASE COUNT          305 a          233 c          282 g          262 t
ORIGIN      
        1 atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt tgttgcagag cttggtgatg
       61 attcaggctg gcattttttc agccagagaa acccggataa cagcatagtg ttcactgtta
      121 acggacaggt aattccgtta aattacggaa acttcgatgc ccgctataaa tatcgaacag
      181 agggggtaca ggatgtacgg tatggccatg aaatgtatta cagccccggc agtaacaccg
      241 tttcgtggag attttgcgca ccttcgggac acgggctgtc agggatggcg atatcggata
      301 ccggccgtaa ctcagcggat aacgttgacg gtgtgtatta ccgaccactg caaaaactga
      361 ttaatggcac ctggtataac gtagcgagta tttaacaatg ttgcatttaa aaaatattac
      421 tgcgggtaat ccgaaaacag cagaacaata tcagatgaca aaacgatatt cggtcacctg
      481 gcttttttca gaagacggaa aaaactggta tgaagagctg aagaatttcg ccagcgacac
      541 aataaaaata gcttacaccg gagatggtcg cgtggtgtgg gtcggtaagg atgtgacagg
      601 catcgagcca cgcaatgcca gcgttatcga agttccggat attaccacta accgacggat
      661 taccgcgccg ggttactggt tttaccgcaa tgatgaattt gtctttgact acagactcaa
      721 agcggaagat gagcgtgatg cccttctggc tcaggtcagt gcccggacag gggaatggga
      781 agaagacctg ctgctggggt taatcagcga cgaagataaa gaaaagctga aagcctgtcg
      841 tatttacgcg aaatcgctgc aggcgatgga tttcagcacc atcactgata aatcctcata
      901 caacgccatt gaatggcccg cctctccgga aggttcttcc tgatttaatt tatcgcgaga
      961 aaaacaatgt ctgtagtgat atcaggtgcg ctgactgatg gcgcaggtat ccccatgtcc
     1021 ggataccata ttattctgaa atcccgggta aacaccccgg aagtggtgat gaacactgtt
     1081 gc
//
LOCUS       DAJTNV010000252         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNV010000252 DAJTNV010000000
VERSION     DAJTNV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>1050
                     /locus_tag="OO199_004113"
     CDS             <1..>1050
                     /locus_tag="OO199_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537932.1"
                     /translation="EENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARDE
                     ELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDAD
                     YTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLA
                     RSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAIVVASVKEAP
                     IGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKT
                     QPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIP
                     PKNPVDADKLLAALR"
BASE COUNT          283 a          285 c          263 g          219 t
ORIGIN      
        1 tgaggagaac atgctcctgc cagtttccgg tcaggaactg cccattcgct ggcttgcgca
       61 acacggcagc gaaaaaccag taacgcacgt ttcacgcgac gaactccagg cattacacat
      121 tgctcgggat gaagaactac cagctgttac tgccctggct gtttcccaca aaaccagcct
      181 gctcgatccg ctggaaattc gcgatctcca caaactggtg cgtgatactg acagagtttt
      241 ccctaatcca ggcaattcaa gcctggggct gatgactgct tttttcgaag catacatgga
      301 cgcagactac accgatcgcg gtctgctgac aaaagagtgg atgaaaggaa atcgtgtttc
      361 acgcatcacg cgcacggctt ccggcgctaa tgctggcggc gggaacctca ccgatcgcgg
      421 cgaaggtttc gttcacgatc tgacgtcact ggcgcgcgac gtagccactg gcgtactggc
      481 tcgttcaatg gacgtggaca tttataacct tcatccggca cacgctaaac gtgtcgagga
      541 aattatcgct gaaaataaac cgcccttttc tgttttccgc gacaaattca tcaccatgcc
      601 tggcgggatg gattattccc gcgccatcgt ggttgcgtca gtgaaagaag caccgattgg
      661 tatcgaggtc atccccgctc acgtcactga atatctgaac aaggtactga ctgaaaccga
      721 tcatgccaac cctgatccgg aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat
      781 gccgcaacgt gtaacagaag aaggaaaaca ggatgatgaa gaaaaaacgc agccatctgg
      841 cgcaatggca gatgaacagg caacggctga aacaatggaa ccggatgcaa ctgaacatca
      901 tcagaacacg cagccgctgg atgctcagtc acaggtaaat tctgttgatg agaaatatca
      961 ggaactacgg gcagaactcc atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga
     1021 tgcagacaaa ttactggctg ccttgaggta
//
LOCUS       DAJTNV010000253          973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNV010000253 DAJTNV010000000
VERSION     DAJTNV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO199_004114"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO199_004114"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5537933.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          257 a          219 c          217 g          280 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ata
//
LOCUS       DAJTNV010000254          971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNV010000254 DAJTNV010000000
VERSION     DAJTNV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.258"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            67..357
                     /locus_tag="OO199_004115"
     CDS             67..357
                     /locus_tag="OO199_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5537934.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            354..908
                     /locus_tag="OO199_004116"
     CDS             354..908
                     /locus_tag="OO199_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5537935.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          263 a          204 c          281 g          223 t
ORIGIN      
        1 gttccgtaaa ttttcctctc cggaggaagt cgaaaatgtg gccgcgcagt tactggagtt
       61 tgcgtaatgg tggatttacg taaagcggcg cgggggcaga tgtgcaccgt cagaattcct
      121 ggctactgca atcacaatcc cgaaacttct gtgctggcgc attacaggct ggcggggacg
      181 tgcggaacag cgacaaaacc acacgatatg caggcagcga ttgcctgtag ctcatgccac
      241 gatttaatcg acgggcgggt aaaaaccagc gattacacca aagaagaatt acgcctgatg
      301 catgcagaag gtgtttttcg cacacaagaa atctggagaa aggaaggtta tttatgattt
      361 acccaacaaa tacaggcaaa agcggggaac accttcgtct caccacgctg gaaagtgtct
      421 ggattcaggg aaaactgcgc atgtgggggc gctggtcgta tattggcggc ggtaagacgg
      481 gaaatatgtt taaccagttg ctggcatcca aaaaattgac gaaaacagcc gtcaatgaag
      541 ccctgcgcag gatgaaaaaa gcgggaatag agaaacctga gctggaagcg tttttgcgag
      601 agatgatcaa tggcaagcaa aagacctggc tggcgcattg tactgatgca gaggcgttat
      661 gtattgatag agtcataagt gaggtgctgg cagagcatcc tggattgatt agtgtcctcc
      721 ggcaacggta tgaagggcgg gggatgacta agcgtaaaat ggctgaattg ctgaatgacg
      781 cacaccctga gtggtgtttc cggacgtgct gcagtcgggt agatgtatgg ctaaatcttg
      841 ctgaatatat gctctatcta ccgatgcgtg atgcattctc ttccggggat ctaaaaaccg
      901 tttgttgact caatctgtta tccggggcta tattcctcac gcgccagcaa aatctggcgt
      961 cgggattggc g
//
LOCUS       DAJTNV010000255          910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNV010000255 DAJTNV010000000
VERSION     DAJTNV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.259"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..910
                     /locus_tag="OO199_004117"
                     /pseudo
     CDS             1..910
                     /locus_tag="OO199_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          244 a          229 c          259 g          178 t
ORIGIN      
        1 gttgaccaga aatacacggt ggcagatgcc gccaaagcta tggatgttgg cctttccaca
       61 atgacaagat gggtcaaaca actgcgtgat gagcgtcagg gcaaaacacc aaaagcctcc
      121 cccattaccc cggaacaaat tgaaatccgt gagctcagga aaaagctaca acgcattgaa
      181 atggagaatg aaatattaaa aaaggctacc gcgctcttga tgtcagactc cctgaacagt
      241 tctcgataat cgggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg
      301 gggttcatcg cagcagctac agatactgga aaaaccgtcc tgaaaaacca gacggcagac
      361 gggctgtatt acgcagtcag gtacttgagc tacatggcat cagccacggt tcggccggag
      421 caagaagcat cgccacaatg gcaacccgga gaggctacca gatgggacgc tggcttgctg
      481 gcaggctcat gaaagagctg gggctggtca gctgtcagca gccgactcac cggtataaac
      541 gtggtggtca tgaacatgtt gctatcccta actaccttga aaggcagttc gccgtgaccg
      601 agccaaatca ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt
      661 acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct
      721 cgccggacag caggctcacc atgaaagcgc tggaaatggc atgggaaacc cgtggtaagc
      781 ccggcggggt gatgttccac agcgatcagg gcagtcatta tacgagcagg cagttccggc
      841 agttattgtg gcgataccag atcagacaga gtatgagccg gcgcggaaac tgctgggata
      901 acagcccaat
//
LOCUS       DAJTNV010000256          905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNV010000256 DAJTNV010000000
VERSION     DAJTNV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.260"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            226..>905
                     /locus_tag="OO199_004118"
     CDS             226..>905
                     /locus_tag="OO199_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537936.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDA"
BASE COUNT          262 a          208 c          237 g          198 t
ORIGIN      
        1 agttcatcaa agcatttatc cactccatca atgtctttcc ttctgtgaat ttttctttgc
       61 tatttaaccg tgatacgact taatttaggc ttaaacacct tacatttaca aggtactcct
      121 gtggggggcc ctgccacgga gcgtcgcagg agtgagtgat gaggaagaat taaagcaacc
      181 tattgtgaca ggcagcgata acagtaaatc acaggaagca taatcatggc aaaaccggac
      241 tgggaggcca tcgaatcggc ataccgggcc ggagtcctta gcctccgtga tataggcgag
      301 aaatacggcg ttacagaagg ggctatcagg aagagggcca aaaagcttga ttgggcacgc
      361 agtggcggta cgcaggtttg caaaaatggt acgcaaaaaa ggaaagtgcg taccagcaga
      421 aagcctgcca ttactggcct tacacaaaaa agtacgcaac taaaaacaga atctacaccg
      481 gatacgaaac cgatacgcgg aatgcgtacc gatcccccga ctaacccatt ccaacccggt
      541 aaccagcagg cattaaaaca cggtggttat gcccgtcgcc ttctgctcaa agatgaggtg
      601 atagaggacg ctaaagcgtt gacgctcgag gacgaattat ttcgccttcg tgctaacaac
      661 cttgtcgctg cagagaatat tggtcggtgg ttggtgtcgc tggaagatgc taatggggac
      721 caggaaagga agatgctgat tgaaaatatc agcgccgccg agaaagcaat gatgcgcaat
      781 acagttcgta ttgagtccat cgttggcacg cttgcgacgg taggaaaaat atttgctgat
      841 acagcctacc gcaaggccgc cactgataag gtgtctctgg aggctgatcg tcttcgccgt
      901 gatgc
//
LOCUS       DAJTNV010000257          889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNV010000257 DAJTNV010000000
VERSION     DAJTNV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..161)
                     /locus_tag="OO199_004119"
     CDS             complement(<1..161)
                     /locus_tag="OO199_004119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537937.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVP"
     gene            208..889
                     /locus_tag="OO199_004120"
                     /pseudo
     CDS             208..889
                     /locus_tag="OO199_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          229 a          242 c          266 g          152 t
ORIGIN      
        1 ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc agcacgcgca
       61 gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc ttcaaaagat
      121 gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc ctctaaggcg
      181 acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc agaatccgcg
      241 gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt cgtatctctg
      301 gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac caacagtgca
      361 tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga aacaaacaag
      421 aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac tgcgccaaag
      481 gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat tgctgccctc
      541 gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc gctgggcaac
      601 gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc gaaagatgcc
      661 actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta ttttacgggg
      721 aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct tgcgaaatcg
      781 accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa ggctgataac
      841 gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaa
//
LOCUS       DAJTNV010000258          859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNV010000258 DAJTNV010000000
VERSION     DAJTNV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(40..180)
                     /locus_tag="OO199_004121"
     CDS             complement(40..180)
                     /locus_tag="OO199_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537938.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            319..>859
                     /gene="queE"
                     /locus_tag="OO199_004122"
     CDS             319..>859
                     /gene="queE"
                     /locus_tag="OO199_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5537939.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTD"
BASE COUNT          242 a          174 c          206 g          237 t
ORIGIN      
        1 gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga taaataaatt
       61 gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag aaaaaataaa
      121 tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt cttctgacat
      181 aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta aatctgcatg
      241 taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt aacaattatc
      301 cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac cctgcaaggt
      361 gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg cccggttggc
      421 tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga agtctccctt
      481 ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag cagtgaagac
      541 ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat tacgggtggt
      601 gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa cggttttagc
      661 tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac ctgggttacc
      721 gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc actggagcga
      781 gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact ggatgaacta
      841 ctggcaacgc tgaccgatg
//
LOCUS       DAJTNV010000259          847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTNV010000259 DAJTNV010000000
VERSION     DAJTNV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..621)
                     /locus_tag="OO199_004123"
     CDS             complement(<1..621)
                     /locus_tag="OO199_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR5537940.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFD"
BASE COUNT          228 a          226 c          202 g          191 t
ORIGIN      
        1 gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa
       61 atccatcgca ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg
      121 ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc
      181 cttaagcaac tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt
      241 gagtttggaa aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac
      301 cggagaaatg agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc
      361 ttctctgatc agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg
      421 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
      481 aaaccagata acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg
      541 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      601 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc
      661 cagagcgcaa agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt
      721 tatgcaaaaa taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt
      781 agccatttag cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta
      841 ttacaaa
//
LOCUS       DAJTNV010000260          811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNV010000260 DAJTNV010000000
VERSION     DAJTNV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>811
                     /locus_tag="OO199_004124"
     CDS             <1..>811
                     /locus_tag="OO199_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5537941.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJTNV010000261          783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNV010000261 DAJTNV010000000
VERSION     DAJTNV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            237..740
                     /gene="dps"
                     /locus_tag="OO199_004125"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="OO199_004125"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5537942.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT          236 a          179 c          167 g          201 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcg
//
LOCUS       DAJTNV010000262          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNV010000262 DAJTNV010000000
VERSION     DAJTNV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..193)
                     /locus_tag="OO199_004126"
     CDS             complement(<1..193)
                     /locus_tag="OO199_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537943.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIY"
     gene            218..457
                     /gene="relB"
                     /locus_tag="OO199_004127"
     CDS             218..457
                     /gene="relB"
                     /locus_tag="OO199_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5537944.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            457..744
                     /gene="relE"
                     /locus_tag="OO199_004128"
     CDS             457..744
                     /gene="relE"
                     /locus_tag="OO199_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5537945.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          210 a          158 c          198 g          205 t
ORIGIN      
        1 agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaaag agctgacctg
       61 cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca gtgatcaccg
      121 ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg tagtgcgata
      181 cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta acctgcgtat
      241 tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg taactccttc
      301 tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc cgttcaaaca
      361 gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac ggcttcgtaa
      421 tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct ggattttgac
      481 gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca gttgaaaaag
      541 aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg tggtatgcct
      601 gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca ggttatagac
      661 gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga agtatatagc
      721 gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc a
//
LOCUS       DAJTNV010000263          690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNV010000263 DAJTNV010000000
VERSION     DAJTNV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..258)
                     /locus_tag="OO199_004129"
     CDS             complement(<1..258)
                     /locus_tag="OO199_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5537946.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQ"
     gene            complement(258..623)
                     /locus_tag="OO199_004130"
     CDS             complement(258..623)
                     /locus_tag="OO199_004130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537947.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
BASE COUNT          168 a          194 c          171 g          157 t
ORIGIN      
        1 ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg caggagcggc ttttacaccg
       61 gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa caacaccact gcgtgtagcc
      121 catcagtgtg gtcccgcagg ccatgatttt tgtcactgat tcaattacca ccggacggac
      181 tgccccttcc ggttgtttct ccagccagtt aagccagcgg tttccctgct gaaaaatatc
      241 ggcaaaacgg gggagcatca gaagggcggg gcgactccgt ccggccagtg aaccgtgcca
      301 cactccgggc agtacatacc gccggcgctg ataccggcaa gaatggtcgc aaattcccgc
      361 tccgtgcagc gggcgatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
      421 agctttttgt ttcccgcatc cacaaagaac ggaatattca ggtctgcgca gcattcaacg
      481 gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct
      541 tcgtgaagct tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta
      601 cggggtttag ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta
      661 taacggtaat tgcagaccat gacaagccgc
//
LOCUS       DAJTNV010000264          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNV010000264 DAJTNV010000000
VERSION     DAJTNV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
BASE COUNT          210 a          165 c          130 g          167 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga
      421 gtgtgttccc ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca
      481 ccagaatgat gcattatcag tcccctgcca gaggttcact acgtaacctg accgacaatg
      541 caccattctg gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag
      601 gagatggtaa ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc
      661 gtctcaaatc tc
//
LOCUS       DAJTNV010000265          652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNV010000265 DAJTNV010000000
VERSION     DAJTNV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>652
                     /locus_tag="OO199_004131"
     CDS             <1..>652
                     /locus_tag="OO199_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5537948.1"
                     /translation="TLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLE
                     FLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLS
                     KVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLAC
                     YQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDST"
BASE COUNT          225 a           97 c          141 g          189 t
ORIGIN      
        1 ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg tatgtgggaa
       61 tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt ctttctccta
      121 gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat tttagcaaac
      181 gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct tattctaaag
      241 atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg gagcgagcgg
      301 atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa gggattcgtg
      361 aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt tatgaaggga
      421 agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag gttctggcgt
      481 gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat cagaagttaa
      541 ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag ttaaataatt
      601 cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct ac
//
LOCUS       DAJTNV010000266          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTNV010000266 DAJTNV010000000
VERSION     DAJTNV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>647
                     /locus_tag="OO199_004132"
     CDS             <1..>647
                     /locus_tag="OO199_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5537949.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNV"
BASE COUNT          209 a          122 c          159 g          157 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtgg
//
LOCUS       DAJTNV010000267          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNV010000267 DAJTNV010000000
VERSION     DAJTNV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>626)
                     /locus_tag="OO199_004133"
     CDS             complement(<1..>626)
                     /locus_tag="OO199_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91-like element ISEc37 family transposase"
                     /protein_id="HCR5537950.1"
                     /translation="GRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRIT
                     RLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGS
                     YLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRM
                     VRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPL"
BASE COUNT          138 a          194 c          135 g          159 t
ORIGIN      
        1 tcagcgggtc aaccttcagt aacctctgat acatccctct ccaggtgatt tgcatcgccg
       61 tttttctcac tgtctccgtt atgatgtaca ccacttcttc cagtaaccgc cgtttcgccg
      121 gactcaaaaa accgtagtac ctcaccatac ggaacccctt atccgccaca tgccaggaga
      181 acctttccat gaactcatct ccactcatca acaggtattc ttcccgtttt gttcggtgac
      241 tgttgtaacg cagaccgatt tcatcctgac cggcataatg ctccagacga ctcatcggca
      301 ccggtggctt tttcaggtaa gagccaaagt acaccgccac atgggtggca ttatccatta
      361 cccgggatac gttgacattc cagccacggc ggtaatgcgt gtccaggaag cgattccatt
      421 cccgtttact gcttccttct gctgccagcg catccggcat caccaggtca gggtatttcc
      481 gtgacagcaa ccgtgttatc cggtagcgcc acatgctcat caccttacgg gcgtaaaaat
      541 gaagattttt ccaggtgtgg cccgacgtca caccaccggc agttgtcgat aaatggatat
      601 gcggatgcca ctgctggtca cgcccc
//
LOCUS       DAJTNV010000268          616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNV010000268 DAJTNV010000000
VERSION     DAJTNV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>616)
                     /locus_tag="OO199_004134"
     rRNA            complement(<1..>616)
                     /locus_tag="OO199_004134"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          115 a          204 c          141 g          156 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctact
      541 agctaatccc atctgggcac atccgatggc aagaggcccg aaggtccccc tctttggtct
      601 tgcgacgtta tgcggt
//
LOCUS       DAJTNV010000269          584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTNV010000269 DAJTNV010000000
VERSION     DAJTNV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..584
                     /locus_tag="OO199_004135"
                     /pseudo
     CDS             1..584
                     /locus_tag="OO199_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          135 a          144 c          180 g          125 t
ORIGIN      
        1 taaagaactc cagcgcctgc tcggcaagaa aacgatggaa aatgaactcc tcaaagaagc
       61 cgttgaatat ggacgggcaa aaaagtggat agcgcacgcg cccttattgc ccggggatgg
      121 ggagtaagct tagtcagccg ttgtctccgg gtgtcgcgtg cgcagttgca cgtcattctc
      181 agacgaaccg atgactggat ggatggccgc cgcagtcgtc acactgatga tacggatgtg
      241 cttctccgta tacaccatgt tatcggagag ctgcccacgt atggttatcg tcgggtatgg
      301 gcgctgcttc gcagacaggc agaacttgat ggtatgcctg cgatcaatgc caaacgtgtt
      361 taccggctca tgcgccagaa tgcgctgttg cttgagcgaa aacctgctgt accgccatcg
      421 aaacgggcac atacaggcag agtggccgtg aaagaaagca atcagcgatg gtgctctgac
      481 gggttcgagt tctgctgtga taacggagag agactgcgtg tcacgttcgc gctggactgc
      541 tgtgatcgtg aggcactgca ctgggcggtg actaccggcg gctt
//
LOCUS       DAJTNV010000270          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNV010000270 DAJTNV010000000
VERSION     DAJTNV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            28..>565
                     /locus_tag="OO199_004136"
     CDS             28..>565
                     /locus_tag="OO199_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537951.1"
                     /translation="MTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGS
                     RAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLL
                     DNVWTVPKEHSKMGNVIRR"
BASE COUNT          153 a          131 c          150 g          131 t
ORIGIN      
        1 tattttgaag agaaggtgga gaactccatg acggtcgccc agtgtctcga ctactggttc
       61 gacaactacg tctctacaac tctcagagaa aagacccagg cactttatcg atcaacggtt
      121 atgaagcgca tgcatgacgc cttccctaat cgtccggcat cttctatcac ggttaagcaa
      181 tgggttgacc tgcttaccga agaagaaaaa gataatccac gccgagcaag gcaggtgcta
      241 agtcaactaa gatcagcaat tagttggtgc atgcggcgtc agttgataga tagttgcgca
      301 attatgagca tccagccaag ggacttcggc tcccgcgctg aggtagggga tcgggtactg
      361 tcgtatcacg aactggctaa gatttggctt gctattgaaa gaagccgtgc gtctacgtca
      421 aataagctcc ttcatcagat gcttatgctg tggggggcga ggctctcaga gcttaggctg
      481 gcaacaaaga cagaatttga cctgctggac aacgtatgga ccgtaccgaa agagcatagc
      541 aagatgggta atgttatccg ccgtc
//
LOCUS       DAJTNV010000271          549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNV010000271 DAJTNV010000000
VERSION     DAJTNV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(96..>549)
                     /gene="malG"
                     /locus_tag="OO199_004137"
     CDS             complement(96..>549)
                     /gene="malG"
                     /locus_tag="OO199_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5537952.1"
                     /translation="LGEYIPFIGLNTHGGVIFAYLGGIALHVWTIKGYFETIDSSLEE
                     AAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDVNSYTLAVG
                     MQQYLNPQNYLWGDFAAAAVMSALPITIVFLLAQRWLVNGLTAGGVKG"
BASE COUNT          152 a          148 c          150 g           99 t
ORIGIN      
        1 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
       61 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
      121 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
      181 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
      241 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
      301 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
      361 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
      421 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
      481 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
      541 tcacccaga
//
LOCUS       DAJTNV010000272          542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNV010000272 DAJTNV010000000
VERSION     DAJTNV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(196..>542)
                     /locus_tag="OO199_004138"
     CDS             complement(196..>542)
                     /locus_tag="OO199_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5537953.1"
                     /translation="EYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKA
                     ELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQK
                     AAFSKQSIAKVD"
BASE COUNT          140 a          140 c           97 g          165 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tatcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc
      421 ctgcgccgcc agccagatta attttttcag gcgttcctgt gttttctctg caattttcct
      481 agtgccagaa tgagccttaa actcttccca cgcccagtgg gttatggtga tctgatactc
      541 cc
//
LOCUS       DAJTNV010000273          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNV010000273 DAJTNV010000000
VERSION     DAJTNV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(108..>538)
                     /locus_tag="OO199_004139"
     CDS             complement(108..>538)
                     /locus_tag="OO199_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5537954.1"
                     /translation="TKSTLLISAILPPASIMMATVITVSKTTLSPWEAGITVMSGAWC
                     GTIVNYHLGIIIGHIPQLACIISSRSNTIERVRLQLQNNSVSILFTSRFIAVLRYIAP
                     LVAGMLQLHPVKVYTVSLISAASWSALYVGSFSFVLPFFS"
BASE COUNT          165 a          108 c          127 g          138 t
ORIGIN      
        1 ccaatgcctt ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt
       61 cagaggaatg ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa
      121 ggcaacacga agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga
      181 ctcactgtat acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg
      241 taacgcagca cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac
      301 tgaaggcgaa cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg
      361 atatgcccaa ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc
      421 atgaccgtga ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg
      481 gccatcataa ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtga
//
LOCUS       DAJTNV010000274          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNV010000274 DAJTNV010000000
VERSION     DAJTNV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>516
                     /locus_tag="OO199_004140"
     CDS             <1..>516
                     /locus_tag="OO199_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5537955.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLT"
BASE COUNT          113 a          163 c          139 g          101 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgtt
//
LOCUS       DAJTNV010000275          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNV010000275 DAJTNV010000000
VERSION     DAJTNV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            134..>506
                     /locus_tag="OO199_004141"
     CDS             134..>506
                     /locus_tag="OO199_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5537956.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEI"
BASE COUNT          158 a           90 c          107 g          151 t
ORIGIN      
        1 atccatgaaa ctactatccg ctctttggat gcatatcctg tacactcaga acatcagaat
       61 ttaaattaag gtttgatgta ttctctgaat aaaacagtaa tgataatttt ggtagaggca
      121 ttcgcactaa ataatgaaaa aatataaatc tgagtttatt cctgaattta agaaaaatta
      181 cctttcccct gtttactggt ctacatggtt ccttttggga atgattgcag gtatttcaat
      241 gtttccccct tcattcagag atcctgtctt ggcaaaaata gggcgttggg cgggcagatt
      301 gagcaaaaaa gctcgtcgca gggcgacgat taatttatcg ctttgtttcc cagaaaagag
      361 tgatacagaa cgggaaataa ttgtcgacaa aatgtttgtc acagcattgc aatccatagt
      421 gatgatggca gaactggcaa ttcgtggtcc ggaaaagttc cagaaacgag tgttctggaa
      481 aggcctcgaa attcttgagg aaattc
//
LOCUS       DAJTNV010000276          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNV010000276 DAJTNV010000000
VERSION     DAJTNV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..305)
                     /locus_tag="OO199_004142"
     CDS             complement(<1..305)
                     /locus_tag="OO199_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5537957.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          158 a           98 c          101 g          149 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcga
//
LOCUS       DAJTNV010000277          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNV010000277 DAJTNV010000000
VERSION     DAJTNV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            200..496
                     /locus_tag="OO199_004143"
     CDS             200..496
                     /locus_tag="OO199_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5537958.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          137 a          111 c          111 g          138 t
ORIGIN      
        1 aaaactaggc ctcgatttta aatgcaaata gcaatcatat tcaataaggc aagacgatca
       61 ggatcagtga aaatgtcatt tttggcgtaa ttacccttac tttatgcatg accttaacaa
      121 agacatcatc tttccgttgc agatcttcgc cctttcaaat aacgtacttt acaactttcc
      181 cgaacaagga gttgtgcccg tgttgtatgt tatttacgct caagacaaag ctgatagcct
      241 cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt ttacagttac tgcatgatga
      301 aggtcgtttg ttgacagctg gtccaatgcc agcggtagac agtaacgatc caggggctgc
      361 gggatttact ggctcaaccg ttattgctga atttgaatcg ctggaagcag ctcaggcctg
      421 ggccgatgct gacccgtacg tagcggcagg agtctatgaa cacgtttcag tgaaaccatt
      481 taagaaagtg ttctgag
//
LOCUS       DAJTNV010000278          496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNV010000278 DAJTNV010000000
VERSION     DAJTNV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..156
                     /locus_tag="OO199_004144"
     CDS             <1..156
                     /locus_tag="OO199_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537959.1"
                     /translation="GEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMP
                     KAPEAGA"
     gene            156..461
                     /locus_tag="OO199_004145"
     CDS             156..461
                     /locus_tag="OO199_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537960.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
BASE COUNT          153 a          103 c          132 g          108 t
ORIGIN      
        1 ggagaaggct ggctaattgg ctggcgttct ctctggtaca cctggggaca aaaagaaacc
       61 ggagaatggc agtggacatt tcaggtcggg gaccttgaaa acgtcaatat cactcactgg
      121 gcagtaatgc caaaagcacc ggaggctgga gcataatgac cacttttacc gacaaagaac
      181 tgattaaaga aattaaagag cgtatcagca gccttgacgt gcgagacgat attgagcgcc
      241 gtgcttatga aatcgcactc ctatctctgg aagtagaacc agatgaacgc gaagcttatg
      301 aattattcat ggaaaagcgt tttggtgact tagtagatcg tcggagagca aaaaacggcg
      361 ataacgaata catggcatgg gatatgactc tcggttggat cgtctggcag caacgagctg
      421 gtatccattt ctcaacaatg tcacagcaag aggtgaaata atggagccat acagcctcac
      481 actcgatgag gcctgt
//
LOCUS       DAJTNV010000279          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNV010000279 DAJTNV010000000
VERSION     DAJTNV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            1..491
                     /locus_tag="OO199_004146"
                     /pseudo
     CDS             1..491
                     /locus_tag="OO199_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          128 a          137 c          130 g           96 t
ORIGIN      
        1 ccagtaaaaa accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg
       61 ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct
      121 acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt
      181 ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc
      241 tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      301 acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      361 ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca
      421 ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg
      481 cctgacggat g
//
LOCUS       DAJTNV010000280          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNV010000280 DAJTNV010000000
VERSION     DAJTNV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>484)
                     /locus_tag="OO199_004147"
     rRNA            complement(<1..>484)
                     /locus_tag="OO199_004147"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a          144 c          118 g          121 t
ORIGIN      
        1 ggtgtgtaca aggcccggga acgtattcac cgtggcattc tgatccacga ttactagcga
       61 ttccgacttc atggagtcga gttgcagact ccaatccgga ctacgacgca ctttatgagg
      121 tccgcttgct ctcgcgaggt cgcttctctt tgtatgcgcc attgtagcac gtgtgtagcc
      181 ctggtcgtaa gggccatgat gacttgacgt catccccacc ttcctccagt ttatcactgg
      241 cagtctcctt tgagttcccg gccggaccgc tggcaacaaa ggataagggt tgcgctcgtt
      301 gcgggactta acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc
      361 acggttcccg aaggcacatt ctcatctctg aaaacttccg tggatgtcaa gaccaggtaa
      421 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
      481 attc
//
LOCUS       DAJTNV010000281          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNV010000281 DAJTNV010000000
VERSION     DAJTNV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
BASE COUNT          132 a           96 c           85 g          156 t
ORIGIN      
        1 tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta ccctcatcgg
       61 taaaaaaatt gcctttcgac cattacgata cattattgat tttgggattt cagggaagta
      121 atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt tgctactaaa
      181 tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg ttatatccac
      241 atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt tgattgttta
      301 atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat atggttattt
      361 ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta gtgcgggtgc
      421 gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DAJTNV010000282          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNV010000282 DAJTNV010000000
VERSION     DAJTNV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..123)
                     /gene="glnH"
                     /locus_tag="OO199_004148"
     CDS             complement(<1..123)
                     /gene="glnH"
                     /locus_tag="OO199_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="similar to periplasmic-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5537961.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFK"
BASE COUNT          151 a           94 c          102 g          122 t
ORIGIN      
        1 tttaaattca aacggaacga aggcggtatc cgtcgcgaca actaattttt tatccgcggc
       61 atgagaagaa accgcaaaag ccagggtcag tgcagccagt gaaactttta atacagactt
      121 catagcattt ccttttttct atccacggga cgatcccctg cgtgacattc acatatatga
      181 aaaaatcgtg ccaattttgc aactcattga tttttcgaga tgcggcaaaa acgtgatgca
      241 cgatttatag ggcaataccc tgaagatgca ccattctggg gcaccaatct ggtgcgctaa
      301 aattgtgcac tcaacacagt gattatttag cgtaaaacct gatgaaaaaa caatctttat
      361 gtaacgattg tgtgatgatg tggatacaaa aaatttactt aatcagctgg ggatagcaga
      421 tggatgcact aaataagtgc gttgaggtgg gctgcaaaac aaaacggcc
//
LOCUS       DAJTNV010000283          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNV010000283 DAJTNV010000000
VERSION     DAJTNV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(119..>467)
                     /gene="csgA"
                     /locus_tag="OO199_004149"
     CDS             complement(119..>467)
                     /gene="csgA"
                     /locus_tag="OO199_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5537962.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          120 a          111 c          113 g          123 t
ORIGIN      
        1 ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata acgcattcat
       61 attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa atgatgtatt
      121 aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg acggaggagt
      181 tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta acagtcattt
      241 cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca cgttgggtca
      301 gatcgattga gctgtcatct gagccctgac caacatctgc accattacca ccgccatgct
      361 gggtaatagt caagtcagag ttacgggcat cagtttgcag agcaagtgca gagttaccgc
      421 caccgtactg gtaaatgttc agctctgaat ttgggccgct attatta
//
LOCUS       DAJTNV010000284          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNV010000284 DAJTNV010000000
VERSION     DAJTNV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..386)
                     /locus_tag="OO199_004150"
     CDS             complement(<1..386)
                     /locus_tag="OO199_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5537963.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVT"
BASE COUNT          114 a          114 c          114 g          119 t
ORIGIN      
        1 gttacccact cgcggaaagc gtcaaacgac tttagcaatg ccgtatcccg ggttctgcat
       61 gtcgcaactg tattaaggta ttgctctgcg gcatcactca gggctggagt gtgttcccga
      121 cctactgatt cgcacaggta atcaacgaag cctgatacca gttttcgttc gcgaggcgtg
      181 atcgccccac cgaccggagt ccagtaatca aatcccagtt gcaggagttt gaaaaaacgc
      241 ttgtggaatg cgtagttacg cacacgctta aagtctgcgt gtatccactc acctattttg
      301 atttgatgca aaaaatcgca actctccggc gtcgccggga gaagtaatcc ggaagaggtt
      361 tgtttgacca gttgtatatg cgccatcgta gttctccgct ggcgcagtag aatgggtgtt
      421 cagcccgtta tgtagtatac cagaattaat gccaatacta a
//
LOCUS       DAJTNV010000285          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTNV010000285 DAJTNV010000000
VERSION     DAJTNV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..97
                     /locus_tag="OO199_004151"
     CDS             <1..97
                     /locus_tag="OO199_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5537964.1"
                     /translation="LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            90..>453
                     /gene="ssuA"
                     /locus_tag="OO199_004152"
     CDS             90..>453
                     /gene="ssuA"
                     /locus_tag="OO199_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5537965.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPI"
BASE COUNT          103 a          114 c          125 g          111 t
ORIGIN      
        1 tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg ttcaggttcc
       61 tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg gcgctggcgg
      121 gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa gcgttacgta
      181 taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag ttactggaaa
      241 aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg caaatgttgg
      301 aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg ccaatctttg
      361 cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag cccaaagccg
      421 aagtgattct ggtggcagaa aacagcccga tca
//
LOCUS       DAJTNV010000286          449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNV010000286 DAJTNV010000000
VERSION     DAJTNV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            57..>449
                     /locus_tag="OO199_004153"
     CDS             57..>449
                     /locus_tag="OO199_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5537966.1"
                     /translation="MQNAENVSEYLSLRHQGLQQSIQSLQSHITSLLADIREIADCQE
                     QVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSGKRKEQQMLEEENSAQPRER
                     NAFAFFHDIFGTGNQHDRMRDFDPDSPEI"
BASE COUNT          127 a          110 c          128 g           84 t
ORIGIN      
        1 agagctccca ttccagagcg gcggcactgg cggttgattc tgccgccgca aaactgatgc
       61 agaatgcgga aaatgtcagc gaatatctgt cattacgcca tcaggggcta cagcaaagca
      121 ttcagtcact gcagtcgcat atcaccagcc tgcttgcgga tatccgggaa atcgcggact
      181 gtcaggagca ggtgaccgct gatgtcagaa tggccatgga ggagatcgat gccagaaccc
      241 gggaattact gacgggggtc tgcacctcac tggaagagga gctgaatgac tatttcagaa
      301 gcggtaaacg caaagaacag caaatgctgg aggaagaaaa ttcagcgcag ccccgggaga
      361 gaaacgcatt cgctttcttt cacgatattt tcggaaccgg aaatcagcat gacaggatgc
      421 gggactttga tccggacagc cccgaaata
//
LOCUS       DAJTNV010000287          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNV010000287 DAJTNV010000000
VERSION     DAJTNV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..134
                     /locus_tag="OO199_004154"
     CDS             <1..134
                     /locus_tag="OO199_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="NAD(P)-binding; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR5537967.1"
                     /translation="GEIIATADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            244..440
                     /locus_tag="OO199_004155"
                     /pseudo
     CDS             244..440
                     /locus_tag="OO199_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT          116 a           91 c          140 g           93 t
ORIGIN      
        1 aaggcgagat tatcgctacc gccgacgcgc ataaggcaac gcgtattgat gcggagctgt
       61 cgatggtggc attgcgggaa tatagagaaa agtttccggc atggcgggat gcggatgagt
      121 ttaggttgtg gtgagtgggg gttgtgttta gggccggggg ctgggctaga ttacgaaagc
      181 ccctaactta aacacaatat tacgccagaa gtcaccattt ccaaaacaca gcgtcgctgt
      241 accgtgggtt atctgcccaa ccgcagcgac acgccggtga tcaaaatcag cgccaaatgg
      301 ctgagaaaag cgggatttga gatcggcacg ggcgttaccg tgaagatctc ggagggatgt
      361 ttgatcctgc ttgggataac aacgaagtac aggaactgcg cagggaactt tatcaggtta
      421 aacagccagt taaggggatg
//
LOCUS       DAJTNV010000288          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNV010000288 DAJTNV010000000
VERSION     DAJTNV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>436)
                     /locus_tag="OO199_004156"
     CDS             complement(<1..>436)
                     /locus_tag="OO199_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR5537968.1"
                     /translation="NRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFN
                     YWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKL
                     TKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT           98 a          112 c           84 g          142 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggtt
//
LOCUS       DAJTNV010000289          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNV010000289 DAJTNV010000000
VERSION     DAJTNV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..159
                     /locus_tag="OO199_004157"
     CDS             <1..159
                     /locus_tag="OO199_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5537969.1"
                     /translation="PSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRLSESVP
                     CMPHKKPG"
     gene            180..>419
                     /locus_tag="OO199_004158"
     CDS             180..>419
                     /locus_tag="OO199_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR5537970.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIRETK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLT"
BASE COUNT          136 a           96 c           99 g           88 t
ORIGIN      
        1 cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt caatgtttac
       61 ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag aagactatct
      121 gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt gcaaaatcaa
      181 ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga
      241 aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt gaccggatcc
      301 gcgagaccaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa atactgctca
      361 gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca ttactgact
//
LOCUS       DAJTNV010000290          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNV010000290 DAJTNV010000000
VERSION     DAJTNV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..150)
                     /locus_tag="OO199_004159"
     CDS             complement(<1..150)
                     /locus_tag="OO199_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR5537971.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVW"
BASE COUNT          131 a           69 c           92 g          111 t
ORIGIN      
        1 ccagacacag aaggcaagaa gtagacaact gactgatatc cagaggtttc ttcgagcaat
       61 atgttttcct ttattttccc agaaggccgg attttctggt ttccagtcgc gtaaaagata
      121 acgactattt ttctcatttt gcagtgccat attgttcctc atatgcacac attggtaatg
      181 aaaaaagaca aaacaggagg taaggcgcaa tagccagtta ttagaattaa ggatgaattg
      241 ggtgaagtgc tgattgaaag aatagataag aaagcgtaac cgcgggggca gaatggggat
      301 taagtagcca gatatgtgtt actaaatgta actaacaagc taaccttcaa acgggggtta
      361 atctttgaac gtatgtcatg tttcaaccct tcagatcgtg aat
//
LOCUS       DAJTNV010000291          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNV010000291 DAJTNV010000000
VERSION     DAJTNV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>401
                     /locus_tag="OO199_004160"
     CDS             <1..>401
                     /locus_tag="OO199_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5537972.1"
                     /translation="LTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDID
                     LKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEV
                     KQREYGRSTVHKCTFVFSPQVIKQRQFSGPH"
BASE COUNT          124 a           87 c           91 g           99 t
ORIGIN      
        1 cctcacccgt gacgaattta ttcgttttat tgaggcttgc cgtcatcaac aaacaaaaaa
       61 cctgtggatt atcgctgtat acacgggtat tcgtcacggg gagctggtat cactggcctg
      121 ggaagatata gaccttaaag cgaggactat aaccatccgc cggaattata caaaactagg
      181 cgaattcact ccaccaaaaa ctgatgctgg taccggaaga acgattcatc tagttcaacc
      241 agctattgat gctcttaaaa gccaagcgga aatgaccatg cttggaaaac agcattctgt
      301 agaggtaaag cagagggaat atgggagaag tactgtgcat aaatgcactt ttgtttttag
      361 tcctcaggta ataaaacagc ggcagttttc cggaccgcac t
//
LOCUS       DAJTNV010000292          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNV010000292 DAJTNV010000000
VERSION     DAJTNV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(55..243)
                     /locus_tag="OO199_004161"
     CDS             complement(55..243)
                     /locus_tag="OO199_004161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537973.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT           97 a           79 c           96 g          127 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggta
//
LOCUS       DAJTNV010000293          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNV010000293 DAJTNV010000000
VERSION     DAJTNV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
BASE COUNT          108 a           94 c           70 g          114 t
ORIGIN      
        1 tctcactgtc taaaattatt gcttctgacc taactgcctc tgacgaccac gcatacgttt
       61 gaggctgaat cctgatgcca aagctttcat cagcagaata atttcctctg ctgaaatggc
      121 aggagctgac actaacactt acgcactggc ctaaaaagtg aagaccgact ctacaggttc
      181 cagcctgaca tgctataaat tttgagtgcc agagcccttg cattaaataa tgcggtttca
      241 acttcattca ccattacatt atcaaatacc cagccataaa cctgtctgat gacagagtct
      301 tctgctgtaa tgtttgtgtc aataccattg caggtgttat taccagttac tcgtatattt
      361 ctcagataac ggccgatagt gtctga
//
LOCUS       DAJTNV010000294          383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNV010000294 DAJTNV010000000
VERSION     DAJTNV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..92
                     /locus_tag="OO199_004162"
     CDS             <1..92
                     /locus_tag="OO199_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5537974.1"
                     /translation="HATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            89..>383
                     /locus_tag="OO199_004163"
     CDS             89..>383
                     /locus_tag="OO199_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5537975.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSP"
BASE COUNT          118 a           89 c          103 g           73 t
ORIGIN      
        1 tccatgctac tggaggggat cgcgtggcaa catcccaaac ggacagaacg gcctggcatc
       61 cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg atattgagct
      121 gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct gtcaggtcgc
      181 gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc gtcgtatgtt
      241 gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc gacaggcaga
      301 aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc tggaagatgc
      361 atcgtcagtc acagattcac ctg
//
LOCUS       DAJTNV010000295          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNV010000295 DAJTNV010000000
VERSION     DAJTNV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..269
                     /locus_tag="OO199_004164"
     CDS             <1..269
                     /locus_tag="OO199_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5537976.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>379
                     /locus_tag="OO199_004165"
     CDS             266..>379
                     /locus_tag="OO199_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5537977.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDP"
BASE COUNT           97 a          100 c          107 g           75 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccg
//
LOCUS       DAJTNV010000296          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNV010000296 DAJTNV010000000
VERSION     DAJTNV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>374
                     /locus_tag="OO199_004166"
     CDS             <1..>374
                     /locus_tag="OO199_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5537978.1"
                     /translation="VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLE
                     PKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSAL
                     GYRSPREYLRQRACNGLSDNRCL"
BASE COUNT           96 a           86 c          116 g           76 t
ORIGIN      
        1 gtcatgctgg gagcggtgga acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag
       61 tggctgacgg ataatggttc atgctaccgg gctaatgaaa cacgccagtt cgcccggatg
      121 ttgggacttg aaccgaagaa cacggcggtg cggagtccgg agagtaacgg aatagcagag
      181 agcttcgtga aaacgataaa gcgtgactac atcagtatca tgcccaaacc agacgggtta
      241 acggcagcaa agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt
      301 gcgctgggtt atcgctcgcc acgggaatat ctgcggcagc gggcttgtaa tgggttaagt
      361 gataacagat gtct
//
LOCUS       DAJTNV010000297          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNV010000297 DAJTNV010000000
VERSION     DAJTNV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
BASE COUNT          115 a           70 c           79 g          106 t
ORIGIN      
        1 ctaacccttc aagaatattc tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg
       61 gagataagtc attgataaaa tggcggagag agggggattt gaacccccgg tagagttgcc
      121 cctactccgg ttttcgagac cgatgataca gagtcgaaaa atcaaagagt taaaaatttt
      181 tcttggaaaa aatgtcttaa aatcgtacac gaaaaatcaa tgaattacat aacattaaag
      241 aagaatattc caagccttct tgagtccacc acctgttttc aggtgtcatt cagacgtgat
      301 ttgccccaga ttgggaaagc acgctttttt ctcagcttat tccgttttgc ctgtgtgttg
      361 aataattcca
//
LOCUS       DAJTNV010000298          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNV010000298 DAJTNV010000000
VERSION     DAJTNV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
BASE COUNT          120 a          103 c           52 g           95 t
ORIGIN      
        1 gcatttcatc tccatctgcg gcacacgcaa ttgccaaagc atatcgatac ggcacattcc
       61 cctgcccctg ctggcacaat aacatctacc agctactctt caacccaaca ccccaaatgc
      121 atttctttat aaatttaatc tgtaacaaaa ccacttccca tatagaaata tttgcaaaaa
      181 tagtgcatga aaaaagcttt tccctaccaa tccgggatat aaataatcat cagtaccaca
      241 attattccac agcaataaat ctcttcccgc cgctccggac ctctgataat ggtctaaaat
      301 cattgaggcc acttgcgacg acattgctca atgaggaaag tttggcgtga ataatattca
      361 acaccagtgc
//
LOCUS       DAJTNV010000299          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTNV010000299 DAJTNV010000000
VERSION     DAJTNV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO199_004167"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO199_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5537979.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTNV010000300          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTNV010000300 DAJTNV010000000
VERSION     DAJTNV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>343)
                     /locus_tag="OO199_004168"
     CDS             complement(<1..>343)
                     /locus_tag="OO199_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5537980.1"
                     /translation="QKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPE
                     DVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRY
                     RAKSLTFYGING"
BASE COUNT           76 a           97 c           89 g           81 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc tgt
//
LOCUS       DAJTNV010000301          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNV010000301 DAJTNV010000000
VERSION     DAJTNV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..189)
                     /gene="torD"
                     /locus_tag="OO199_004169"
     CDS             complement(<1..189)
                     /gene="torD"
                     /locus_tag="OO199_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5537981.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
     gene            complement(186..>341)
                     /locus_tag="OO199_004170"
     CDS             complement(186..>341)
                     /locus_tag="OO199_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537982.1"
                     /translation="GTSQLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYV
                     PASQVKS"
BASE COUNT           68 a          109 c           84 g           80 t
ORIGIN      
        1 cgtggcaata ctgttttcca gatcattcac cgccgcagtg agcggcggtt cgcttttcag
       61 caacgaaaac cattcagcca tctgcgcact ggcgatttgc gtcagttgtt catcgtccag
      121 ttcacgggag aacaactgcg ccagccaggc gtaaacacag gcaatctgtt gtgctgtcag
      181 cgtggtcatg atttcacctg cgacgcggga acatattcgc actgcgccac catctccacg
      241 gggccgttaa acgccgtcac ctgctccact gttccattgt acttctcaat ttccaccagc
      301 gtagtgtgcg cactggccgc ctgcgccagc tgcgaagtcc c
//
LOCUS       DAJTNV010000302          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTNV010000302 DAJTNV010000000
VERSION     DAJTNV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>336
                     /locus_tag="OO199_004171"
     CDS             <1..>336
                     /locus_tag="OO199_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5537983.1"
                     /translation="NVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQT
                     SQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGED
                     GIAYVSGLSA"
BASE COUNT           97 a           61 c           94 g           84 t
ORIGIN      
        1 aacgttaatg gctggccggg actgagtact gattttcgtg ggtatgcaat actgccagtg
       61 cagccatacc gaagagacga tgttattctg gatgaaaaaa cgataggtaa gaattacgac
      121 ttaccacaaa ctagtcaatt ggttgtaccg acagctggag ccgttgtgcc tgcgacattg
      181 gcagtgaaga gcggggataa aggattagta accttaaaac agaaagaggg taaacccgtc
      241 cccttcggcg ctgtgattag ttatagcaaa gacactgaaa atatggccgg cattgtaggt
      301 gaagatggta ttgcttatgt ttctggcttg agtgcg
//
LOCUS       DAJTNV010000303          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNV010000303 DAJTNV010000000
VERSION     DAJTNV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            36..>336
                     /locus_tag="OO199_004172"
     CDS             36..>336
                     /locus_tag="OO199_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5537984.1"
                     /translation="MEGGGIFTVGDECHAHFLVEQGKEAKIALEQAGLLVLAIRTPLI
                     RKLKQEKPGELGEIARVLAENNINILVQYSDHANQLILITDNDSMAASVTLPWAIK"
BASE COUNT          114 a           51 c           85 g           86 t
ORIGIN      
        1 tactaggaaa aacattaggt aataaaggta ttggattgga agggggaggg atatttacgg
       61 ttggtgatga atgccatgct cattttcttg ttgaacaagg aaaggaagct aaaatagcgc
      121 tagagcaagc tggactgtta gtacttgcga tccggacacc attaattcgt aagttaaaac
      181 aggaaaaacc gggggaactt ggcgaaatag cacgagtatt ggcggagaat aacattaata
      241 ttttagtgca atacagtgac catgctaacc aactgatatt aataacggac aatgatagta
      301 tggctgcatc tgttacgctc ccttgggcaa taaagt
//
LOCUS       DAJTNV010000304          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNV010000304 DAJTNV010000000
VERSION     DAJTNV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(128..>333)
                     /gene="tabA"
                     /locus_tag="OO199_004173"
     CDS             complement(128..>333)
                     /gene="tabA"
                     /locus_tag="OO199_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5537985.1"
                     /translation="TQPAGAPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEV
                     HKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT           96 a           94 c           60 g           83 t
ORIGIN      
        1 cccatgacga actccttgag ttaataagaa cgtctaagca ttacaaattt tactcacaaa
       61 aaaatatcaa aaaacgctct tttcacaaac tcgccgtaaa cctggagtta tttacggcga
      121 aaagtcatca cgccatcagc atcttcacta ctgctttgcg aacctgggct ggcgcgccca
      181 ctgcgcacag cggtttatgc acttcccccg gataaaacac aacaaaatca ccttcattta
      241 ggataactgt tttctcatca acgccttccg gcaaaaatgc gatgtcttta tcagctaacc
      301 agtcggtatc cggcgcgcct gcaggttgcg tgc
//
LOCUS       DAJTNV010000305          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNV010000305 DAJTNV010000000
VERSION     DAJTNV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..>326)
                     /locus_tag="OO199_004174"
     CDS             complement(<1..>326)
                     /locus_tag="OO199_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5537986.1"
                     /translation="GLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIA
                     PPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPR
                     PQLQFS"
BASE COUNT           82 a          101 c           79 g           64 t
ORIGIN      
        1 tcgctaaatt gcagctgtgg acgcggttta accgacgcgg agagatacgt ccgttcttcg
       61 tcagtcagga caaactgcgc ccggttgcga taccacgcgt accaacagat cccaaccaga
      121 atccctgcca cgccgatgat aacgaacatc gtccgccagc cccatgccag ctgcatcaat
      181 acaagagcag gaggtgcgat agcctgaccg ataacggtag atgagttaaa aatccccagc
      241 gccgtgccgc gttctttttg tgcataccag tcggtgatcg actttactcc cgcaggcata
      301 aatggcgctt cgccaatacc cagacc
//
LOCUS       DAJTNV010000306          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNV010000306 DAJTNV010000000
VERSION     DAJTNV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..>312
                     /locus_tag="OO199_004175"
     CDS             <1..>312
                     /locus_tag="OO199_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5537987.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     G"
BASE COUNT           92 a           65 c           78 g           77 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg ac
//
LOCUS       DAJTNV010000307          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNV010000307 DAJTNV010000000
VERSION     DAJTNV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..116)
                     /locus_tag="OO199_004176"
     CDS             complement(<1..116)
                     /locus_tag="OO199_004176"
                     /inference="COORDINATES: protein motif:HMM:NF021997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR5537988.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNY"
BASE COUNT           89 a           80 c           63 g           79 t
ORIGIN      
        1 ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt
       61 gttccagcca taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct
      121 ttcctctttt ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt
      181 ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac
      241 ctacctcaat agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta
      301 taaaattcag c
//
LOCUS       DAJTNV010000308         5818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.guided.161, whole genome shotgun sequence.
ACCESSION   DAJTNV010000308 DAJTNV010000000
VERSION     DAJTNV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.guided.161"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO199_004177"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO199_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5537989.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OO199_004178"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OO199_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OO199_004179"
     CDS             2144..2617
                     /locus_tag="OO199_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5537990.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OO199_004180"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OO199_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OO199_004181"
     CDS             2967..3803
                     /locus_tag="OO199_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5537991.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OO199_004182"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OO199_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OO199_004183"
     CDS             complement(4358..4747)
                     /locus_tag="OO199_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5537992.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OO199_004184"
     CDS             4965..5405
                     /locus_tag="OO199_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5537993.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1397 a         1539 c         1519 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggatgaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DAJTNV010000309         2737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.guided.213, whole genome shotgun sequence.
ACCESSION   DAJTNV010000309 DAJTNV010000000
VERSION     DAJTNV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.guided.213"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..283)
                     /gene="tetR(B)"
                     /locus_tag="OO199_004185"
     CDS             complement(<1..283)
                     /gene="tetR(B)"
                     /locus_tag="OO199_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5537994.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHR"
     gene            365..1570
                     /gene="tet(B)"
                     /locus_tag="OO199_004186"
     CDS             365..1570
                     /gene="tet(B)"
                     /locus_tag="OO199_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5537995.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1683..2276)
                     /gene="tetC"
                     /locus_tag="OO199_004187"
     CDS             complement(1683..2276)
                     /gene="tetC"
                     /locus_tag="OO199_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5537996.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2364..>2737
                     /locus_tag="OO199_004188"
     CDS             2364..>2737
                     /locus_tag="OO199_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5537997.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPS"
BASE COUNT          743 a          495 c          559 g          940 t
ORIGIN      
        1 cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc
       61 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
      121 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
      181 ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct
      241 ctaatgcgct gttaatcact ttacttttat ctaatctaga catcattaat tcctaatttt
      301 tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga tagagaaaag
      361 tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg ccatggggat
      421 tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt cggaagatat
      481 cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta tctttgctcc
      541 ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt tgtcattaat
      601 aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga tgctgtattt
      661 aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat cggtcattgc
      721 cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg caagttttgg
      781 gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt caccgcatag
      841 tccctttttt atcgctgcgt tgctaaatat tgtcgctttc cttgtggtta tgttttggtt
      901 ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg agacgcaatc
      961 gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta tttatttttc
     1021 agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt
     1081 tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt tacactcagt
     1141 attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact
     1201 gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat ctgaaggttg
     1261 gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac ctgcattaca
     1321 gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg gattattggt
     1381 gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta tttataatca
     1441 ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt actgtattat
     1501 tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta aacaggagac
     1561 aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat gaccctcttg
     1621 ataacccaag agggcatttt ttacgataaa gaagatttag cttcaaataa aacctatcta
     1681 ttttatttat ctttcaagct caataaaaag ccgcggtaaa tagcaataaa ttggcctttt
     1741 ttatcggcaa gctcttttag gtttttcgca tgtattgcga tatgcataaa ccagccattg
     1801 agtaagtttt taagcacatc atcatcataa gctttaagtt ggttctcttg gatcaatttg
     1861 ctgacaatgg cgtttacctt accagtaatg tattcaaggc taattttttc aagttcattc
     1921 caaccaatga taggcatcac ttcttggata gggataaggt ttttattatt atcaataata
     1981 taatcaagat aatgttcaaa tatactttct aaggcagacc aaccatttgt taaatcagtt
     2041 tttgttgtga tgtaggcatc aatcataatt aattgctgct tataacaggc actgagtaat
     2101 tgttttttat ttttaaagtg atgataaaag gcacctttgg tcaccaacgc ttttcccgag
     2161 atctcatcta ttgaaacagc ttgatagcct ttttcaacaa acaatattcg tgctgagtta
     2221 accagtgatt gataggtact cttaaaattt tcttgttgat gatttttatt ttccatgata
     2281 gatttaaaat aacataccgt cagtatgttt atggtatcat gatgatgtgg tcgtgacaat
     2341 cttaagaaca tttaggttat tttatgtata ttgaacagca ttctcgctat caaaataaag
     2401 ctaataacat ccaattaaga tatgatgata agcagtttca tacaacggtt atcaaagatg
     2461 ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat gtggcgaata
     2521 aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca ggggtcacac
     2581 tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg aggttgacga
     2641 aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa tcttttacac
     2701 gtcgatttaa gtacattttt aaggttacac caagtta
//
LOCUS       DAJTNV010000310         2416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.guided.218, whole genome shotgun sequence.
ACCESSION   DAJTNV010000310 DAJTNV010000000
VERSION     DAJTNV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.guided.218"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            <1..335
                     /gene="frdD"
                     /locus_tag="OO199_004189"
     CDS             <1..335
                     /gene="frdD"
                     /locus_tag="OO199_004189"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5537998.1"
                     /translation="DEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGLFPGDALSYE
                     RVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVV
                     TLIGVVTI"
     gene            398..1531
                     /locus_tag="OO199_004190"
     CDS             398..1531
                     /locus_tag="OO199_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5537999.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1620..2153
                     /gene="blc"
                     /locus_tag="OO199_004191"
     CDS             1620..2153
                     /gene="blc"
                     /locus_tag="OO199_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5538000.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2150..>2416)
                     /gene="sugE"
                     /locus_tag="OO199_004192"
     CDS             complement(2150..>2416)
                     /gene="sugE"
                     /locus_tag="OO199_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5538001.1"
                     /translation="VGLKYTHGFSRLTPSVITVTAMIVSMALLAWAMKSLPVGTAYAV
                     WTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKLSTH"
BASE COUNT          574 a          636 c          627 g          579 t
ORIGIN      
        1 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
       61 cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg
      121 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
      181 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
      241 atctgaaaat ccacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
      301 cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta aatccggccc
      361 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
      421 cttattaatt accgcctctt actccacatt tgctgcccct caacaaatca acgatattgt
      481 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
      541 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
      601 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
      661 gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata
      721 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta
      781 caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct
      841 gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa
      901 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
      961 ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
     1021 tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc
     1081 gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg
     1141 ctgggtgcaa agcaatttac ccccccttga tatcaatgag aaaacgcttc aacaagggat
     1201 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     1261 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     1321 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     1381 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     1441 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     1501 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     1561 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     1621 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt
     1681 ttcctacgcc gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa cgttatctcg
     1741 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     1801 ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc
     1861 ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc
     1921 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac
     1981 tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     2041 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     2101 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     2161 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccaga cgcatcgggt
     2221 tagcggactc accgagcagc acaatgccgg tgatggccgc gccgactgcg ccaatacccg
     2281 tccacacggc ataagccgtc cctaccggta acgatttcat cgcccaggca agtagcgcca
     2341 tactgactat catcgccgtc acggtaataa cactcggcgt caaacggcta aagccgtggg
     2401 tatatttcag gccaac
//
LOCUS       DAJTNV010000311          979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 103229
            SAMEA8899865-rid15821693.guided.256, whole genome shotgun sequence.
ACCESSION   DAJTNV010000311 DAJTNV010000000
VERSION     DAJTNV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899865
            Sequence Read Archive: ERR6005274
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478188.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478188.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:52:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,113
            Genes (coding)                    :: 3,794
            CDSs (with protein)               :: 3,794
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 70
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 148 of 319
            Pseudo Genes (incomplete)         :: 65 of 319
            Pseudo Genes (internal stop)      :: 135 of 319
            Pseudo Genes (multiple problems)  :: 28 of 319
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899865-rid15821693.guided.256"
                     /serovar="S. flexneri 1b"
                     /isolate="103229"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2010-03-24"
     gene            complement(<1..273)
                     /locus_tag="OO199_004193"
     CDS             complement(<1..273)
                     /locus_tag="OO199_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5538002.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDF"
     gene            535..>979
                     /gene="gpt"
                     /locus_tag="OO199_004194"
     CDS             535..>979
                     /gene="gpt"
                     /locus_tag="OO199_004194"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5538003.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPP"
BASE COUNT          252 a          228 c          250 g          249 t
ORIGIN      
        1 gaagtcatgc acggtgtcgt tatttttctg cggcaccata tcgaggtggg cctgtaagac
       61 gaccggttta cgattttcca tacctgcggt agcaggttta cgaatcagga tattacctac
      121 ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa ccaacaatgt attcagcgag
      181 ttgctcttca tgataggacg ggtgaggaat agaacagatt ttggcaaaaa tatcccacag
      241 cggctgtgga gataattgag acagttcaga cacgttaagt ctccttgtcg atcacccgca
      301 aaacagtatt gcaggtcaca gggttagcag aaaatgttgt caacacaaga caggcttgcg
      361 agatatgtat tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
      421 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
      481 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
      541 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
      601 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg
      661 ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
      721 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
      781 ggcttcatcg ttattgatga cctggtggat accggtggta ctgctgttgc gattcgtgaa
      841 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
      901 gatgactatg ttgttgatat cccgcaagat acctggatcg aacagccgtg ggatatgggc
      961 gtcgtattcg tcccgccaa
//
